Biovac 15 Manual
Contents
1. The radius of the rotor and the shape of centrifugal contained is taken into account during the setting of The numerical value of the centrifugal force Setting of running time Setting range of running time of rotors 1min 9hr 59min Press up or down arrow no 2 on Time icon to adjust the preset numerical value The set numerical values cannot be changed while the centrifuge is running Press and hold the relevant arrow for more than one second to add or decrease preset running time Simultaneously the bottom line on main display shows up and down arrows on the left side and preset time on the right side This data will be stored into the system automatically and the interface will return to its original state after the data entry is complete Biovac 15 11 Running of device The Run Sequence will not start until the users data has been set the rotor has been installed the samples evenly distributed and the lid is closed Attention A lid lock icon on the main interface will indicate whether the lid of the device is closed correctly Commencement of Centrifugation Press the start key no 7 on the control panel and the rotor will begin running evenly at a speed of 300rpm while it will simultaneously run a rotor identification program After the rotor has been identified the rotor accelerates according to the preset elevating speed curve line of the preset rotary speed After pressing the start key no 7 on the co2. Rubber sheath of the electrical machine 5 Rotor supporting panel of the electrical machine axis 6 Rotor Figure 12 Sketch map of rotor installed Biovac 15 2 4 2 5 2 6 Maintenance of lock sleeve and shaft Steps to maintain lock sleeve and supporting shaft of rotor e Turn on the unit and wait until the self check has completed e Press button 4 to open the lid of the centrifuge Unscrew the fastening screw lock sleeve of the rotor with the 5mm allenkey supplied then clean it Attention turn clockwise to tighten and anti clockwise to loosen e Clear dust impurities and residue away from the centrifugal chamber e Clean the cone section see Fig 11 e Clean external surface of the lock sleeve of the rotor e Screw the fastening bolt into the screw of the cone section SLOWLY with the 5mm hexagon allenkey Attention moderate tightening is permissible The bolt must not touch the bottom as this could damage the fastening bolt and cone section and as a result you will be unable to mount the rotor The calculation of rotor load Calculation of maximum load capacity At high speed there is enormous centrifugal force each rotor is designed to safely withstand the force generated by the maximum rated rotary speed however the above safety factor is conditional upon the rotor load not exceeding the maximum rated load When separating samples the total weight must not exceed the maximum rated load of the rotor Altern
3. whether the four centrifugal cups are movable by hand The horizontal rotor is not designed to run for prolonged periods at the critical speed of 2000rpm as this may result in major vibration and the working life of the machine will be shortened When changing rotors please unscrew bolts anti clockwise to loosen the rotor Do not start the machine when fastening screws are loose When repeated running is required for the centrifuge re check the fastening screw after the rotor has run several times It is acceptable to load samples into the centrifugal tubes symmetrically at different times Asymmetrical loading is strictly forbidden Parameter setting Attention You should install the rotor in the rotary shaft before setting parameters Note The setting parameter buttons are touch sensitive and need only be touched lightly You may reset the parameters again if mistakes occur in the operation This machine provides users with a selection of 9 different kinds of elevated speed curve lines Switch on the machine When initialization has completed press the up and down arrows no 2 on control panel to set desired parameters The set numerical values cannot be changed while the centrifuge is running Press and hold an arrow button for more than 1 second to add or decrease the preset elevating speed curve line At the same time the lowest line on main interface shows an up and down arrow on the left side and preset elevating speed curve l
4. Biovac 15 Operating Instructions Biovac 15 IMPORTANT Remove all stabilizing bolts before use IMPORTANT Remove all stabilizing bolts before use Operating environment of instruments In order to extend the machine service life please pay attention to the following factors which may damage the machine e Environmental Conditions e Effects of environment including natural ultraviolet radiation ie Direct Sunlight e Erosion and abrasion of the protective cover and other safety parts e Indoor use e Altitude lt 2000m e Ambient temperature 5 C 35 C e Relative humidity lt 80 e Operating voltage 230V 50 Hz e Please ensure adequate ventilation in area of use e Ensure there is no vibration and or a strong draft to influence the performance e Ensure there is no dust or flammable and corrosive gas present in the ambient air Please read this manual carefully when using the instrument for the first time The maintenance of the instrument should be carried out by the agents authorized by Biovac The following materials should not be used e Flammable and explosive substances e Strong chemical materials e Toxic or radioactive substances e Pathogenic microorganisms The Biovac 15 High Speed Bench top Centrifuge can be maintained only by qualified service engineers using appropriate tools Should there be a problem not mentioned in this manual please contact a Biovac authori
5. ately reduce the weight of samples or calculate permissible running speed to ensure the motor load will not exceed its maximum rated load Calculation method of permissible running speed Nperm is as follows Nperm Nmax x maximum permissible load actual load 05 Nmax Maximum rated rotary speed Maximum permissible load Actual load No overloading The rotor may explode severely damaging the centrifuge Sample filling It is critical to ensure the centrifugal container s centrifugal tube etc specifications are within the maximum rated acceleration centrifugal force specifications if necessary reduce the acceleration speed to the recommended specifications of the containers During operation it is important to keep the centrifuge balanced Interference due to the vibration caused by incorrectly distributed samples should be avoided Therefore it is important to evenly fill the centrifugal container with samples All samples should be placed in appropriate containers Observe the recommended operational life of the centrifugal containers used especially those frequently run under maximum permissible load velocity temperature Replace the container plastic glass immediately if damaged Biovac 15 Safe use of rotor Before the rotor runs load samples and place tubes correctly When installing the centrifugal cup of the swing out rotor please check that the pins are in the slots on both sides of the centrifugal cup and
6. ectricity is switched off there is no brake therefore the rotor will continue to run for a long time Please wait for it to stop first Steps to open the lid in case of emergency are as follows e Ensure that the rotors are not running to confirm observe from the eye hole at the top of the lid e Turn off the electricity e Pull out the plastic plug at the bottom of the front wall of the chest with a knife or screwdriver Pull the line connected to the back of the plastic plug to open the lid and then remove the separated samples from the centrifuge Return the line into the device and replace the plug to its original place 16 P O Box 48488 Roosevelt Park 2129 South Africa Tel Jhb 011 475 1823 Dbn 031 301 7617 CT 021 461 9607 Fax 011 475 1819 email support vacutec co za Biovac 15
7. ervation hole Cover Foot Control panel and display window Ventilation holes Power jack Ventilation holes SO 0 AON GU 8 03 10 a LINK LH Jai I LUI Control panel and display interface Data display window Adjustable numeric value buttons Alarm indicator lamp Open button Quick run mode Stop button Start button Rotary speed Centrifugal force swap OOS Yt RO R Biovac 15 2 2 2 2 2 2 0000 00 0000 mat amp e 00000 rpm D Speed 00000 rcf 1 Stand By 6 FL eal 1 Curve line indicator is for rotor and elevating speed the four numbers in the top right corner are rotor numbers in the bottom right corner are the numbers for the elevating curve line Warning indicators for lid lock and running status Display area of time shows remaining running time Type of Current Rotor Displays Serial No Serial No 0000 when rotor is absent Current rotary speed centrifugal force displays current and actual rotary speed of the rotor rpm 00000 for stop displays current and actual centrifugal force g of the rotor rcf 00000 for stop 6 Set indication area of parameter and running status shows stand by when the machine is ready to work shows Running when the machine runs properly Shows relevant preset value when the parameter of temperature rotary speed and elevating speed curve line is present Shows failure reasons whe
8. he performance or even damage the centrifuge Rotors used must be approved by Biovac to avoid malfunction Various types and specification of rotors and centrifugal containers are available from Biovac Please use the provided allenkey when installing rotors Steps to install rotors are as follows see Fig 10 11 12 e Switch the unit on wait until the self check is complete e Press button 4 to open the lid of the centrifuge make sure the inner part of the chamber is clean and clear e Clean the external surface of the rotor lock sleeve e Prepare the desired rotor hold it in both hands insert the rotor centre into the lock sleeve position rotate it vertically on the bottom of the cone then gently press the rotor by hand to ensure the rotor base is in place as indicated on the display panel No 5 shown in figure 9 e Use the 5mm allenkey supplied and turn it clockwise to fasten the screws Do Not over tighten e Mounting of rotors is now complete You should periodically recheck that the mounting is secure before and after operation Tighten the fastening screws again if necessary to ensure the rotor is mounted firmly Figure 10 Sketch map of rotor lock sleeve and supporting axis Figure 11 Sketch map of rotor installation Description of figures 10 11 and 12 L__ jo 1 Fastening screw atl 16 2 Lock sleeve by lt 3 Electrical machine axis cone 4
9. ine on the right side This data will be stored into the system automatically and the interface will return to its original state after the data entry is complete Setting of rotary speed 10 e Rotary speed range of the Biovac 15 High Speed Bench top Centrifuge The minimum rotary speed 300rpm The maximum rotary speed 16 000 rpm e Set rotary speed of rotors by following these steps First see whether there is a dot in front of the O0000rpm as shown in figure 9 if there is no dot press the Speed F key to switch between the values and then press up or down arrows no2 to set the numerical value The set numerical values cannot be changed while the centrifuge is running Press and hold down the relevant arrow no2 on the Speed icon of the control panel as shown in figure 4 for more than one second to add or decrease preset rotary speed simultaneously the bottom line on the display window shows up and down arrows on the left side and the preset rotary speed on the right side This data will be stored into the system automatically and the interface will return to its original state after the data entry is complete Biovac 15 Setting the centrifugal force It is also possible to change the setting of the rotary speed by setting the numerical value of centrifugal force Only if you know the type of rotor may you convert the centrifugal force and rotary speed manually The value of the centrifugal force and rotar
10. l operation of the equipment to prolong the service life and for the accumulation of the operation data of the equipment to assist in diagnosing the problem or cause of an accident The recommended table of operation data is as follows Date of Name Weight of Rotor Rotary Time Temp Velocity Program Working Operator test of monotube No speed Condition sample including sample Cleaning Disinfecting and Maintainance Users have the responsibility to clean and disinfect the centrifuge when dangerous substance spill in the machine Users should clean and disinfect it according to the method recommended by the manufacturers to prevent damage to the machine Inappropriate detergents and incorrect cleaning methods may cause damage to the centrifuge or other internal parts Cleaning and sterilization should be carried out according to operating procedures described in the operating and technical instructions Cleaning Turn off the power switch and unplug from the power source before cleaning The centrifuge shell rotor chamber rotor centrifugal containers etc should be cleaned and 14 Biovac 15 maintained regularly or as determined by the service condition to avoid damage If it is unclear which method or process should be followed for cleaning the centrifuge please contact Biovac Organic solvents are forbidden as they can dissolve the grease in the bearings of the motor Liquid especially organic so
11. lvents should not come in contact with the supporting shaft and ball bearings when cleaning If there is frost or a thin layer of ice it should be wiped away before it has melted Disinfecting Rotors used by this centrifuge can withstand high pressure sterilization at a Temperature of 127 5 C and Absolute pressure of 250kPa maintained for at least 10 mins Please refer to the cleaning instructions of separating containers made by other manufacturers Confirm whether it is permissible to sterilize rotors and containers with high pressure before proceeding The temperature and duration of the high pressure sterilization should be strictly controlled according to the specifications of the different rotors and separating containers in use Dont use the rotor and separating container if they are corroded or damaged Maintainance Dont pierce the rotor with sharp objects When being moved and disassembled you should take care not to damage the rotor as scratches and scars may cause cracks during operation Regularly check whether there are pits grooves or minute cracks in the rotor especially at the base of the test tube If any of the above are found stop using them immediately The rotor should be cleaned once a week for normal usage Rinse the rotor immediately if it is used to separate saline solution or other corrosive samples If any of the sample liquids spill onto the rotor blot it up and rinse the part immediately Please clea
12. n After the parameters have been modified and the main interface menu re appears the revised parameters will come into effect 12 Biovac 15 Termination of the centrifuge operation There are two ways to terminate the centrifuge operation During normal operation the running time has been preset When the run time has completed the centrifuge will stop automatically When the rotary speed of the rotor falls to zero that is RPM 00000 and RCF 00000 you may press the opening button no 4 on the control panel then lift the lid to open it If the state of the lid lock indicates that the tapered end is open then you can take the separated samples out of the centrifuge During normal operation you may press Stop no 6 on the control panel any time to terminate the centrifuge operation and the centrifuge will stop immediately The countdown stops at the same time You can press the open button no 4 to open the lid only when the RPM and RFC centrifugal force displayed fall to zero When the state of the lid lock indicates the tapered end is open you can take the separated samples out of the centrifuge Attention Ifthe centrifuge is stopped mid operation the run time will be cancelled and needs to be reset before the next centrifuge operation Disassembling of rotors Attention When disassembling rotors you should hold and lift the rotors with both hands Vibration is forbidden Steps to disassemble ro
13. n warning is displayed SID Starting Insert one end of the power cable into the supply socket at the bottom right of the back panel the other end is connected to the municipal power supply The voltage of the required power supply is AC230V at a frequency of 50Hz After connecting turn on the power switch at the bottom right of the back panel the buzzer will sound and the icon Heal Force is displayed and a self check is simultaneously carried out When the self check has been completed the main interface appears see Fig 9 The machine is now ready for operation Opening If the electricity is on and the rotor is not running press the Open button on the control panel to open the lid The data display window displays Lift lid By spring action the lid will open automatically and rise to a certain height It then needs to be manually lifted to rest on the frame The inner chamber will now be visible and accessible to the user The icon on the main interface will indicate that the lid is unlocked Closing Lower the lid and press the lid down until an audible click is heard The lid locks by use of a touch travel switch The icon on the main interface will indicate when the lid is properly closed and has been locked When closing the lid do not press forcefully as you could damage the lid lock Biovac 15 2 3 Installation of rotor Inappropriate types of rotors and centrifuge tubes may influence t
14. n with sponges or a cotton cloth dipped in a neutral cleaning solution then rinse the cleaning solution off with distilled water Don t sprinkle or spray water on the rotor because it may cause corrosion on the rotor Invert the rotor to dry it Dust the cooling plates of the condenser once every three months Ensure the protective cover is in place Inspect rotor parts for any unsafe developments Continuity of grounding protection Remove the ice or condensation within the Chamber when present Turn off the electricity and unplug the supply cable connected to the back of the device before disassembling the cover casing on the front of the device Operation is forbidden during maintenance to avoid an electric shock or damage to the device Attention This should be performed only by trained Biovac maintenance personnel Only spares provided by Biovac can be used in this device Turn off the electricity when the centrifuge isn t in use Transportation and storage This machine is a precision instrument Exposure to water vibration tilting and inverting the machine is forbidden in the process of transportation and storage Biovac 15 15 Opening the lid in case of emergency Under normal circumstances do not manually open the lid when the electricity is switched off or the operation to open the lid is non responsive Attention Only in the case of an emergency may you open the lid of the centrifuge by hand to remove samples When the el
15. ntrol panel the run time will begin to count down from preset time When it displays zero that is 00 00 the centrifuge stops and the work is complete The temperature in the chamber falls rapidly from 00 C When the temperature reaches the preset temperature the compressor stops In operation the rotary speed and the centrifugal force see Figure 9 rise independently from 00000 and 00000 until they reach the preset Rotary speed and Centrifugal force and the centrifugal force is converted automatically according to the detected rotor There will be warning prompts if The rotary speed and centrifugal force of the rotor exceed the maximum permissible value or preset value during a temperature protection operation on the electric machinery over voltage operation of the compressor and if an error occurs with the EEROM RAM and so on Warning parameters should be set for each operation Do not open the lid while the rotor is running Imbalance detection If the rotor is unbalanced and the vibration of the rotational shaft exceeds the set range during operation the device will stop at once and a warning prompt will appear The problem should be found and the speed reduced about 500rpm 800rpm The device may be started again once the problem has been resolved Modification of parameter during operation All parameters except the accelerating curve and braking curve can be modified during operatio
16. sportation underneath the unit gt This device should be installed on a rigid table or counter top which is free from vibration and direct sunlight gt There should be a space of 10 15cm around the device for heat emission from the condenser After mounting ensure all four feet are level and supported by the work surface gt Range of operational power supply AC230V 50Hz v You should not disconnect power during the rotor s operation as this will cause damage to the control circuit Configuration 1 2 Transportation and installation Biovac 15 High Speed Bench top Centrifuge is transported in a special purpose wooden case with protective materials inside to assist with cushioning After opening the cushioning should be removed The net weight of the device is about 75kg therefore it is important to lift the entire device left and right to maintain balance Do not hold onto the front cover when lifting Ensure that balance is maintained with minimal vibration Use the special packaging cases when transporting long distance Handle with care 1 3 Appropriate working environment 1 Biovac 15 High Speed Bench top Centrifuge is for indoor use only where conditions should meet the following specifications 2 A safety clearance of 10 15cm must be maintained around the centrifuge When running there should be no harmful substances nearby 3 The work bench should be on a solid floor to avoid vibration if mobile racks or
17. tors are as follows e Stop the centrifuge e Open the lid e Take out the centrifugal container first if the horizontal rotor exists e Unscrew the fastening screw anti clockwise with the 5mm allenkey supplied for disassembling no need to completely remove the screw Hold the rotor with both hands and vertically lift it Vibration is forbidden e Remove the container and rotor from the fastening bolt lock sleeve of the rotor if the sample has been contaminated or to prevent it from being contaminated e Remove the sample container from the rotor in a suitably safe environment For example put the separated sample container and rotor in a chest which is safe for biological operations and open the rotor to remove the sample after being decontaminated Description of error messages The bottom line of the display screen shows all error messages with a X on the left side and the related error messages on the right side Simultaneously a buzzer will sound and a red warning light will flash The sounding alarm will not stop automatically but it can be silenced by the operator by Pressing the no 3 button on the control panel The red warning light will not stop flashing until the problem has been resolved Biovac 15 13 Imbalance warning Keyboard cpu self check register warning Keyboard cpu self check inner ram warning Operation records Please keep records of each operation one set for each device to ensure the norma
18. trolleys are used they should be fitted with locking devices to ensure the safe operation of the centrifuge 4 Should the centrifuge be placed beside a wall or in a corner there should be 10cm between the back of the device and the wall as well as 15cm between the left and right sides and the wall to ensure good ventilation and heat emission 5 The centrifuge should be placed away from windows to avoid the direct heat and radiation of sunlight 6 The centrifuge should always be placed in a clean environment with a constant ambient temperature between 5 C and 35 C and ambient humidity less than 80 Biovac 15 1 4 1 5 2 1 Fixed placement of equipment After the centrifuge has been placed it should not be moved frequently When moving ensure the centrifuge is kept upright balanced and vibration is limited To avoid damage to rotor axis remove the rotor when moving the centrifuge Proper connection of electric supply The supply socket connected must be earthed securely and be in good condition The power wires of the centrifuge should be in accordance with regional safety standards Voltage and frequency of the power supply should be in accordance with the requirements stated in the technical description or specifications labelled on the nameplate of the centrifuge The power jack switch is at the back of the machine indicating I for on and O for off Operating instructions Front cover casing Lid Obs
19. y speed are inversely proportionate The value of the centrifugal force and rotary speed is displayed synchronously after the centrifuge has run for several seconds and the model type of rotor is identified Set the centrifugal force as follows See whether there is a dot in front of 00000 rcf on the main interface as shown in figure 9 if there is no dot press the Speed F button to switch to 00000 rcf and then press the up or down arrow no 2 to set numerical value Press and hold the up or down arrow no 2 for more than 1 second to increase or decrease the value This data will be stored into the system automatically and the interface will return to its original state after the data entry is complete Attention A numerical value of centrifugal force set below the minimum rotary speed of 300rpm in some rotors may lead to the instability of the device Calculation on centrifugal force Generally speaking the relative centrifugal force is several thousand times more than G force g It is the unit to measure the efficiency of various instruments to separate or deposit things The calculation of the centrifugal force is related to the centrifugal rational speed and centrifugal radius The formula for calculation is as follows RCF 11 18 n 1000 2 r r Centrifugal radius unit cm n Centrifugal speed unit rpm Rpm Attention The numerical value of the maximum centrifugal force is related to the maximum radius
20. zed agent for the correct processing methods Please only use accessories provided by Biovac If other accessories are used Biovac will not be held responsible The Biovac 15R High Speed Bench top Centrifuge should be checked and maintained regularly Safety protection device Biovac 15 High Speed Bench top Centrifuge has a series of safety protection devices gt The machine frame is made of steel plate and the protection steel ring is made of steel casing The inner chamber is stainless The front frame is made of ABS gt The structure of the lid is explosion resistant There is a viewport in the center of the lid Only when the centrifuge is on and the rotor has stopped may the be pressed to open the lid Only when the centrifuge lid is locked may you start the centrifuge again gt Overspeed stop gt The warning alarm is sounded when the rotary speed exceeds the preset speed by 200rpm Wait until the rotor stops completely then open the lid Restart when the problem has been resolved Biovac 15 gt Do Not Open the lid while in operation If is pressed by accident while in operation the device wont respond gt Opening in case of emergency If there is something wrong with the rotor and the electricity is off you may open the lid by hand when there is no response when pressing amp see Trouble shooting Settling the centrifuge gt NOTE Remove the stabilizing bolts used during tran
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