MPR 010 Mobile Phase Recycler
Contents
1. INTI MPR 010 Mobile Phase Recycler Version 1 00 User s Manual Revision A May 2000 Copyright 2000 LabAlliance All rights reserved This manual or any of its parts must not be electronically mechanically or photographically copied reproduced or stored otherwise without permission LabAlliance MPR 010 user s Manual TABLE OF CONTENTS TABLE OF CONTENTS sissies casccusessschnsessetanotesseswtiecsbenstssesonsntucesnsugucesssbendusieasnseseoanedesoneadebestensssoesekesesseesdeseso 1 LIST OF FIGURES ocn ssssencsesocstesoasncvessossecedesssnsscsssacessbasnavessescunessssacessaubdebesn nates eO EA E OEDS DE e OOS Ts 2 INTRODUCTION dueno icasnsuesensusstussonsnsnssen due cenduseesenstestasionosetecsstistenenssess 3 SPEGIFICA TION 5 5 4552555c ste bec tab e e e Ae eee 4 INSTALLATION ccc c itsesvssuatessssvsesssenesenestusateshehescssssaeasssadacsbsesssenssossaseesousaeuavonons E O E a S S 6 Liquid connection isiovseiciiaciincneinociieniei eiiiai i E E i 6 Electric l connection neee A A alee cts anes 6 Connection with computer optional ocicicicicuonnnonononnnaninanonanananananananonananononananononananononananonanananananns 8 HOW MPR 010 WORKS i wiccsccscccscsosessstosonessesuesicesssoonstosossncesesnsuencnsnobendasvsesusens ongtecessbentebupeensbeontenesenssedtensen 9 MOBILE PHASE RECYCLING cuide tele ais INPUT RANGE ccccscessessessessesscscesceseeseenecnecns AUTO ZERO VERSUS ACTIVEZERO DELAY AND TRANSPORT2. DELAY c0000 ees ees DIRECT CONTROL MODE sta EE EEEE E E A ace son setts EEES E Eea see ESEE EEE eh FATE SAFE BD D E dd A E EA ete SA A MPR 010 AND GLP ie EN a we MPR O10 FACTORY SETTING sendos MPR 010 STARE UP ssc sesscscasssccesenccsssaussceveosovscncovaceconsnnsesstnscesedeon SVOE EaR e ES O ESEE rO TESSE 11 LabAlliance MPR 010 LIST OF FIGURES user s Manual Figure 1 MPR 010 Mobile Phase RecyCclel ccccceeceeeeeeeees 3 Figure 2 MPR 010 Position in the HPLC System 60055 4 Figure 3 MPR 010 Operational Principle ccc cece cece ees 4 Figure 4 Flange Free FINO vc aa 6 Figure 5 MPR 010 Rear Panel ooooccocccccconcncnnocan cnn cnn rcnn nas 7 Figure 6 MPR 010 Front Panel ccccc cece cece ee eeeeeeeees 11 LabAlliance MPR 010 user s Manual INTRODUCTION MPR 010 Mobile Phase Recycler Figure 1 is a useful cost saving device for isocratic HPLC With efficient recycling of mobile phase one can save up to 90 of solvent Usually most of the mobile phase is free of contaminants and need not be wasted MPR 010 allows the user to redirect the uncontaminated mobile phase to the solvent reservoir saving solvent and waste disposal costs Figure 1 MPR 010 Mobile Phase Recycler MPR 010 continuously monitors the detector signal In response to signal level the 3 way valve is switched to waste or recycle position The switching valve is located ju
3. MPR 010 baseline signal ZERO also activates the ActiveZERO function At the moment of ZERO the ActiveZERO contact closure is closed for the pre defined period 3 seconds by default This ActiveZERO contact closure when connected to the auto zero input of detector sets the detector output to zero After this period when the detector signal is stabilized MPR O10 sets itself to zero The ActiveZERO function allows the user to synchronize the zero level of both detector and MPR 010 with a single button command Delay and Transport Delay The functions of Delay and Transport Delay are very similar The Delay is applied after the peak whereas Transport Delay prior to peak The Transport Delay factory setting is O and can be changed from MPR 010 Control Panel only LabAlliance MPR 010 user s Manual Direct Control Mode The basic method for switching valve position is signal dependent In special cases MPR 010 can be switched directly to WASTE RECYCLE positions They are two ways using front panel buttons WASTE and RECYCLE or external signal WASTE When the MPR 010 valve is under direct control the position LEDs are blinking The direct control mode can be escaped by pressing MODE button Fail Safe Design The MPR 010 was designed to recycle the mobile phase only if active and properly initialized by user e The normal position of the MPR 010 3 way valve is WASTE In the case of power fail the valve remains in the WASTE
4. T LabAlliance MPR 010 user s Manual INSTALLATION It is easy to install MPR 010 for the experienced HPLC user No special tools are required Once the tubings are connected to the valve and detector signal output is connected with MPR 010 the recycler is ready for use An optional computer connection can be made Liquid connections The recycler valve should be placed in the HPLC system according to Figure 2 page 4 The Detector liquid output should be connected with the valve port D the Waste bottle with port W and mobile phase Reservoir with R port Use the flange free fittings Figure 4 and capillaries from the start up kit for the connections A NEO Figure 4 Flange Free Fitting Electrical connections They are different ways to make electrical connections from MPR 010 to the chromatographic system The schematic depends on your specific requirements see following examples LabAlliance MPR 010 user s Manual DC Signal Zoro Waste AciivaZERO Pc ae IN iN IN OUT 9 12 Y _ e E a y Figure 5 MPR 010 Rear Panel Input from the integrator output of HPLC input yellow detector 1Volt Full Scale External autozero command e g injection input black valve autosampler etc input red ActiveZERO Allows the synchronized zeroing of detector and output green MPR 010 prior to sample injection When the ZERO button is pressed or external zero input is shor
5. hreshold setting THRESHOLD LED is lit e Using UP and DOWN arrows set the Threshold value If you are new with using MPR 010 the default value of 0 5 is good choice e Press MODE button again to access the Delay setting It can be calculated by formula Delay s Tubing volume mL Flow Rate mL min Delay Calculator in the MPR 010 Control Panel Software can be also used for the calculation e Press MODE button Display now shows the input signal E ee LabAlliance MPR 010 user s Manual CONTACT FOR TECHNICAL SUPPORT Should you have further questions comments or suggestions concerning the MPR O10 contact your distributor below LabAlliance 349 N Science Park Road State College PA 16803 USA Phone 800 345 5557 toll free within U S 814 234 7317 Fax 814 236 6072 Email sales laballiance com When contacting technical support please provide the following information MPR 010 Mobile Phase Recycler Accessible on the instrument bottom 13
6. position and the mobile phase remains uncontaminated e After MPR 010 is Reset Power On the detector signal is ignored and the ZERO button must be pressed for initialization MPR 010 and GLP MPR 010 GLP functions are available from MPR 010 Control Panel software The Installation Qualification Operational Qualification and Performance Qualification procedures and protocols are available upon request MPR 010 Factory Setting Threshold 0 5 Delay BS 35 Transport Dela ActiveZERO Dela or contact closure peaks 10 LabAlliance MPR 010 user s Manual MPR 010 START UP MPR 010 Controls amt SIGNAL THRESHOLD DELAY RECYCLE WASTE DeltaChrom MPR 010 Figure 6 MPR 010 Front Panel Display Display the current signal level in of full scale Threshold and Delay 000 indicates the zero state Display mode LED SIGNAL signal level THRESHOLD switching level DELAY WASTE to RECYCLE switching dela s Continuous light input signal level LED dependent position blinking direct control mode Activates the ActiveZERO output and performs the MPR 010 auto zero function UP and DOWN Allows the parrameter setting 11 LabAlliance MPR 010 user s Manual arrows ooo T O WASTE button Direct WASTE position RECYCLE button Direct RECYCLE position First steps with the MPR 010 e Connect the power adapter e Press the ZERO button e Press MODE button to access the T
7. st after the detector output Figure 2 If the signal level detected is greater than the set signal threshold the valve is switched to waste position The switching may be delayed slightly to account for transport time from the detector cell to 3 way valve Figure 3 LabAlliance MPR 010 user s Manual Figure 2 MPR 010 Position in the HPLC System MW WASTE EJ RECYCLE THRESHOLD Figure 3 MPR 010 Operational Principle MPR 010 can be connected to any HPLC detector with an integrator output 1 V Full Scale They are two major benefits of using the integrator output 1 better resistance against radio frequency interference and 2 constant signal to voltage ratio independent of the actual range setting The analytical fitting kit 1 16 is a standard part of delivery Optional semi prep 1 8 fitting kit is also available Sali m 0 1 99 of input range 1 990 mV Delay sd 1 99 s with 1 s increment Transport Dela O 99 s with 1 s increment 100 of input range Valve 3 way electrically actuated valve Ya 28 flat bottom fitting 0 2 MPa 30 psi maximum PTFE LabAlliance MPR 010 user s Manual Tous Cr Green LED RECYCLE po te RE aa ActiveZERO contact closure RS 232 control program included Power requirements 115 V 5 VA power adapter MPR 010 itself uses the safe voltage 10 35 C up to 60 relative non requirements condensing humidit 200 x 140 x 55 mm EA
8. ted the HPLC detector is zeroed immediately after ActiveZERO Delay MPR 010 is zeroed itself PC connector allows user to connect the MPR 010 to a computer with Control Panel Software Additional functions are available with PC control MPR 010 is powered by safe voltage from the power adapter Connection Examples 1 MPR 010 will be used independent of the other HPLC system components Using the Universal Cable P N DY2K012507 connect the detector 1V FS output with the yellow connector on the MPR 010 rear panel 2 MPR 010 Auto zero function should be activated at the moment of injection Connect the analog signal as described in example 1 The black MPR 010 connector should be 7 LabAlliance MPR 010 user s Manual connected to the contact closure of the manual injection valve or autosampler MPR 010 will be zeroed at the injection 3 Both detector and MPR 010 should be zeroed manually before an injection Connect the detector signal output to the yellow MPR 010 connector Green ActiveZERO output should be connected to the detector external zero input ZERO button of MPR 010 cause the auto zero function on both instruments Connection with computer optional If advanced functions of MPR 010 are required connect the recycler to a computer Advanced functions Transport Delay setting Using of Delay Calculator Setting of external ZERO mode Detect negative peak setting GLP functions PQ f
9. unctions for authorized technicians only RS232 cable should be connected to the free communication port of your computer COM1 COM 4 can be used Then install the MPR 010 Control Panel Software The program runs under Windows 95 98 NT LabAlliance MPR 010 user s Manual HOW DOES THE MPR 010 RECYCLER WORK Mobile phase recycling MPR 010 continuously watches the detector signal If the signal level exceeds the Threshold the 3 way valve switches the mobile phase flow to the waste bottle After the signal decreases to a value below the Threshold level the valve still holds in the WASTE position for Delay time Then it switches to the RECYCLE position and the mobile phase is recycled Depending on the chromatographic analysis the optimal Threshold setting usually is between 0 2 1 The Delay parameter should match the internal volume of tubing between detector and MPR 010 A useful tool for Delay determination is the Delay Calculator in the MPR 010 Control Panel software Input range MPR 010 Input Range is equal to the standard integrator output range of HPLC detectors 1V MPR 010 analog input accepts both positive and negative peaks However the negative peak detection can be suppressed using MPR 010 Control Panel Options Setting Auto Zero versus ActiveZERO The Auto zero function is activated by pressing the ZERO button or external ZERO signal The current voltage monitored by the analog IN is then used as the
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