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Agilent 218 Solvent Delivery Module

1. Stop Figure 16 57 218 Solvent Delivery User Manual Using 4 Sample Methods eee gp pg o ee ee eee LL LL LL EL LL ___ ee am N ed 2 2 w ox ex wu _____ NOLLISOdWO2 MO14 oun utis Mo9O1Nn 201 sse ov sessed JO ON sduing jo ON Blank Figure 17 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual 60 P ad a Choose the Appropriate Pump Head for the Application e 2 o eo 90 apdnism Performance e This chapter gives hints on how to optimize the performance or use additional devices Agilent Technologies 59 5 Optimizing Performance Choose the Appropriate Pump Head for the Application 60 Choose from standard pump heads providing flow rates up to 10 25 100 or 200 mL min Single Pump Operation Ideally for single pump operation the flow rate should be between 5 6 and 90 of the maximum pump head flow rate Examples 10 mL min pump head for applications requiring between 0 5 9 mL min 100 mL min pump head should be used for applications requiring between 5 90 mL min The pump head can be used at its fully specified range but a 10 mL min pump head will operate at 1 mL min better than a 100 mL min pump head Gradie
2. Aromatic hydrocarbons Figure 13 218 Solvent Delivery User Manual 54 4 Using Sample Methods O ed sishjeuyyy syuawwog _______ 0 mo 3 x _ wes ft ft ft fT S S es fs es IL LL LL ll ft petit ff foo f ee Es ee ee ee 2 2 w ox ex wu 00 81 xoown 6501 2 sauna o on nm medo ssl summ tics Figure 14 AA Analy 55 218 Solvent Delivery User Manual Using 4 Sample Methods eugnoi sis jeuv wv sjera jo no sun Jejduiesojne uueje punog uiu qu 0 dues sjuawwog T gp pg og Jp LL LL L tt CEs ae Fae eS Ee ee ee 2 2 w ox ex wu 00 2 86 62 11 ewg eomg 0 Kqpuejg pow Figure 15 218 Solvent Delivery User Manual 4 Using Sample Methods uwop inus pasn 12ejuo jsnonunuoo spunos wsejy uiuu qui 0 0 ejeipeuuuj
3. Figure 3 Pressure module The following pressure modules are available p n Description 393552501 Analytical pressure module titanium 8700 psi 10 mL 393552801 Analytical pressure module PEEK 4000 psi 10 mL 393552601 Semi prep pressure module titanium 6000 psi 393552901 Semi prep pressure module PEEK 4000 psi 393553001 Preparative pressure module PEEK 2000 psi 100 mL 393552701 Preparative pressure module titanium 1200 psi 100 mL 393650501 Pressure module titanium 4000 psi 200 mL Only one pump in the HPLC system the master pump needs to have a pressure module installed 218 Solvent Delivery User Manual 13 1 14 Introduction The pressure module dampens pump pulsations and supplies the current pressure value to the Agilent 218 pump software The pump needs this information to implement compressibility compensation and flow rate accuracy corrections and to ensure that system pressure is within the limits entered during setup Choose a pressure module that has a pressure and flow limit greater than the maximum pressure and flow you will be using 218 Solvent Delivery User Manual Introduction 1 Pump Head Pump Head Agilent 218 Pump Heads are easily changed self contained units including spring loaded pistons and check valve cartridges Simply loosen a finger tight clamp to rapidly change pump heads between analytical and preparative configurations Figure 4 Pump head installed on the pu
4. 1 Disconnect the module from the mains power supply 2 Locate the fuse box on the right side of the back panel TET Fuse box 3 Inspectthe fuse box to see what configuration your pump 4 Gently pry open the fuse box door To do this use a small is set to Look for the white plastic pip protruding through flat head screwdriver to carefully pry open the top and the hole next to the voltage setting bottom left side and then the top and bottom right side Open the fuse box door by pulling the right side away from the pump and swinging the door to the left Voltage set at 240 V 218 Solvent Delivery User Manual 99 7 Maintenance and Repair 5 Liftthe fuse holder out Remove the two fuses for the 240 V setup and replace them or the single 120 V fuse Figure 24 120 V fuse in holder The fuse holder has a small cutout on the side as shown in the previous figures that fits around a small guide post located in the left side of the fuse box 6 Slide the fuse holder back into the fuse box ensuring that the fuse is facing into the instrument Once inserted the fuse should not be visible For details see instruction sheet p n 8510249500 100 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual 8 659 Parts e o e e e e A Parts List 102 Liquid Heads 103 Pressure Modules 104 Mixer 105 Standard Accessory Package 106
5. 7 Maintenance and Repair Introduction to Maintenance 72 The pumps have been carefully designed with continuous unattended operation in mind Rugged construction and sophisticated electronics mean a minimum of routine maintenance and years of trouble free service if treated carefully and if replacement parts are changed when they show signs of wear This section of the manual describes a maintenance schedule service logs changing the piston seals changing the check valve cartridges and changing the mixer seals and frits You should take advantage of the Service Logs Software in the pump automatically tracks seal wear check valve use and pump drive wear The software also allows the user to enter and record seal and check valve changes Make it a point to check the Service Log area frequently and make sure to record seal changes and check valve replacements into the software 218 Solvent Delivery User Manual Maintenance and Repair 7 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice The volume of substances should be reduced t
6. 2 Use 100 6 methanol at 25 mL min for 5 10 min You can recycle the methanol back into the supply reservoir 90 218 Solvent Delivery User Manual Maintenance and Repair 7 Breaking In a New Seal Heads 200 mL min When After seal replacement To maximize the life of new seal 1 Run the pump without backpressure for 1 2 min at 20 of nominal flow rate with 100 methanol 2 Plumb a column or tubing or restrictor that causes approximately 13 8 MPa 2000 psi and 20 7 MPa 3000 psi pressure into the HPLC system and run the pump at normal operating pressure for 5 10 min checking for any leaks 3 Repeat Step 1 for 30 minutes The seal will now be ready for normal operation 218 Solvent Delivery User Manual 91 7 Maintenance and Repair Cleaning Check Valves When Occasionally especially in case of drop in backpressure A pressure drop may indicate that one of the check balls has become coated with gummy or particulate matter or that a small particle has become lodged on the seat in either case the check ball will not seat correctly and pressure will be lost It may be possible to rectify this problem using isopropanol to dissolve the foreign matter Cleaning Check Valves with Isopropanol 1 Disconnect the outlet tubing and connect a line to a waste bottle 2 Check miscibility of solvent in the pump head with isopropanol 3 Isopropanol and solvent in pump head miscible Pump isopropanol in the pump hea
7. Replacing Piston Seals Heads lt 200 mL min 86 Replacing Piston Seals 200 mL min Head 88 Breaking In a New Seal 200 mL min Head 90 Breaking In a New Seal Heads 200 mL min 91 Cleaning Check Valves 92 Replacing Check Valves 94 Checking and Replacing the Mixer Outlet Filter Frit Analytical and Narrowbore Mixers Only 96 Replacing the Mixer Seal 97 Checking and or Changing Power Fuses F1 98 Parts 101 Parts List 102 Liquid Heads 103 Pressure Modules 104 Mixer 105 Standard Accessory Package 106 218 Solvent Delivery User Manual Contents 9 Cables 107 Cable Overview 108 Cable Connections 109 10 Appendix 111 General Safety Information 112 Solvent Miscibility 118 Solvent Compressibility 119 The Waste Electrical and Electronic Equipment Directive 120 Batteries Information 121 Radio Interference 122 CECompliance 123 Electromagnetic Compatibility 124 Agilent Technologies on Internet 126 218 Solvent Delivery User Manual Contents 8 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual 1 659 Introduction e e e d Introduction to the System 10 Physical Layout 12 Pressure Module 13 PumpHead 15 Check Valves 17 Dual Chamber High Pressure Mixer 19 Control 22 This chapter gives an instrument overview n Agilent Technologies 1 Introduction Introduction to the System 10 A complete modular system includes the pump s tubing
8. This chapter provides information on parts for the instrument RE Agilent Technologies 101 8 Parts Parts List p n Description G9300A Agilent 218 Isocratic Solvent Delivery Module G9301A Agilent 218 Add on Solvent Delivery Module 102 218 Solvent Delivery User Manual Liquid Heads p n R007101061 R007101062 R007101063 R007101064 R007101073 R007101074 R007101076 R007101077 393650701 218 Solvent Delivery User Manual Description Standard head max flow 10 mL min Washing head max flow 10 mL min Washing head max flow 10 mL min Standard head max flow 2b mL min Washing head max flow 10 mL min Washing head max flow 25 mL min material SST material SST material Titanium material SST material PEEK material PEEK Washing head max flow 100 mL min material PEEK Washing head max flow 100 mL min material Titanium Washing head max flow 200 mL min material Titanium Parts 103 8 Parts Pressure Modules p n Description 393552501 Pressure module flow 10 mL min max pressure rating 8700 psi wetted material Titanium FEP 393552601 Pressure module flow 50 mL min max pressure rating 6000 psi wetted material Titanium FEP 393552701 Pressure module flow 100 mL min max pressure rating 1200 psi wetted material Titanium FEP 393552801 Pressure module flow 10 mL min max pressure rating 4000 psi wetted material PEEK FEP 393552901 Pres
9. c Loosen the two screws 17 at the front of the pump head and remove the bearing housing 6 and the housing spacer 7 d Remove washing section 8 o ring 12 and seal backups 9 13 6 Carefully remove the seals 10 14 If the seals are stuck pry them out carefully so that the liquid head 11 or washing section 8 is not scratched Do not reuse a seal after it has been removed 84 218 Solvent Delivery User Manual Maintenance and Repair Removing seals 200 mL min Head Removing seals 200 mL min Head Tools required Description 1 4 in hex wrench 3 mm wrench 1 Turn off power to the pump 6 Place the liquid head with both inlet and outlet check 2 Remove pump head clamp by loosening the hex screw valves plugged with 1 4 in hex wrench Lift off clamp and pull pump head out of the housing Unscrew the threaded cap see Figure 19 on page 81 and remove all parts possible from this side of the head 5 Before loosening the two hex head screws draw a line the length of the pump head body using a marker pen This line will make it easier to have all of the parts in the correct orientation during reassembly Loosen and remove the two 3 mm hex head screws and separate all of the parts 7 Fillthe cylinder cavity half full with HPLC grade water 8 Push down on the piston The piston seal will pop out Insert the ceramic piston into the cavity through the onto the piston piston seal 218 Solvent Del
10. 0 00 5 2 8 psd uru qu A 0 0 00 5 00 0 2 9 upu qu up A 00 2 eur3 5 A OA 0 A SNIT 440 4015 YIN N34SNVHL Nuva onnaa 3201 HLAW T 0 0 SdON AL3JVS H34SNVHL SS320V SSVd WIEN 40 AdOD u39eW MAN WA A 9ILVNAWVHOSVIG GOHLAW gos 0 0 unu Gp Meu 104 896001 1 o eu 20 896001 01 uiu 92 Meu J0j y d y 8 92 1e 02 Meu 104 8 5 0 dureg uiu Meu 104 05 1e pjoH 01 euijouun MAU JO 8950s 0 suonipuoo Meu pue Meu JO 5 55024 JORIUOD JOS uiu qu S M0J4 suonipuoo 195 00 0 euin 10 mouy UMOG SSeJd Suonipuoo erui 0 40j eum 195 Kex SSeJd se poule Ajayeg pue 1965 jeg MIN SSAA Kex GOHLAW 5924 Method editing diagrammatic form Figure 8 45 218 Solvent Delivery User Manual 4 Using Method Menu Display the Main Method display 1 Press the METHOD key then the NEW ke
11. 2 ElL S 5 o a L 00 S lt lt lt m m O 5 d e a 2 6 Ek Figure 26 Solvent miscibility of some common solvents 118 218 Solvent Delivery User Manual Appendix 10 Solvent Compressibility The values in Table 29 on page 119 should be used for the Agilent 218 Purification Solution compressibility factors when you are setting up the pumping system parameters For details on how to set up the pump system parameters refer to Adjusting the Flow Rate on the Pump on page 79 Table29 Compressibility factors Solvent x L Water 46 3231 Acetone 128 9 956 Acetonitrile 97 4 1212 Benzene 96 7 1046 Carbon tetrachloride 106 7 998 Chloroform 97 4 1227 Cyclohexane 114 800 Dichloroethane 111 9 1020 Diethyl ether 188 700 Dimethylformamide 80 1500 Dioxane 60 1500 Ethanol 115 1100 Ethyl acetate 100 1800 Methylene chloride 97 4 1212 Methanol 125 1200 n Heptane 144 760 n Hexane 167 2 644 Dichlorobenzene 95 1400 Propanol 98 1200 Tetrahydrofuran 95 1500 Toluene 93 1200 2 Methylformamide 80 1500 218 Solvent Delivery User Manual 119 10 Appendix The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13
12. Agilent 218 Purification Solution The current method will stop and transfer to the selected method If there is no STOP method selected the pump will abort the running method and stop HIGH P Used to set the method to be used in the event of a High Pressure condition High Pressure Limit or Max Pressure is set in the Pressure menu The current method will stop and transfer to the selected method If there is no HIGH P method selected the pump will transfer to the Stop Safety method if one is specified otherwise it will simply stop when the High Pressure Limit is reached 50 218 Solvent Delivery User Manual Using 4 Table 10 Safety methods Setting Use case LOW P Used to set the method to be used in the event of a Low Pressure condition Low Pressure Limit or Min Pressure is set in the Pressure menu The current method will stop and transfer to the selected method If there is no LOW P method selected the pump will transfer to the Stop Safety method if one is specified otherwise it will simply stop when the Low Pressure Limit is reached OFF LINE Used to set the method to be used in the event that one of the other pumps in the system goes OFF LINE The current method will stop and transfer to the selected method If there is no method selected the Agilent 218 will transfer to the Stop Safety method if one is specified otherwise it will simply stop if another pump goes OFF LINE If you do not wish the method to t
13. Agilent 218 either by entering a number between 0 and 63 or pressing the UP ARROW or DOWN ARROW key to scroll through a preset list of choices Available choices are 0 63 MC master controller or ID HdSz Pump head size Use the UP ARROW or DOWN ARROW to select between a preset list of choices Choices 5 10 25 50 100 and 200 mL min 10P 25P 50P 100P The P designation stands for PEEK The compressibility compensation for PEEK heads is different than for stainless steel or titanium heads x Compressibility Factor This is used to calculate the flow rate compensation necessary to correct for the solvent s compressibility Values can be set between 0 2000 Default of 46 is the setting for water The parameter can be adjusted to set the measured flow rate at exactly the set flow rate See Adjusting the Flow Rate on the Pump on page 79 for details on how to do this Other solvents will have different x parameters See Solvent Compressibility on page 119 for a list of values for other solvents L High Pressure Constant Range is from 1 9999 bar Default is 3231 bar the value for water Consult the available literature for high pressure constants for other solvents A partial list is given in Solvent Miscibility on page 118 REFILL Refill time in milliseconds Refill time is the time required for the piston return stroke Range is from 100 1000 ms CIM Control Interface Module ID Set the
14. August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex this product is classed as a Monitoring and Control Instrumentation product E Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 120 218 Solvent Delivery User Manual Appendix 10 Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning m kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandgren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterije
15. Guide p n G9300 90300 If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired 218 Solvent Delivery User Manual Using 4 Power On Power On When you switch on the power the Agilent 218 Purification Solution will perform a self check and display the screens below in the order shown Solvent Delivery System Version x x Version x x Self Test Self Test Ram OK Pressure Module 8700 psi Appropriate pressure rating will be shown 5 time mL min psi 0 00 0 00 0 gt 218 Solvent Delivery User Manual 41 4 Using Priming the Pump Heads Tools required Preparations 42 Pump heads are shipped dry IPA methanol or HPLC grade water is recommended for priming the pump heads and pre wetting the seals Description Syringe Pump head size entered see Setting the Pump ID and Pump Head Size in Agilent 218 Purification System Setup and Installation Guide p n G9300 90300 1 Remove the outlet tubing and fitting from the outlet check valve 2 Usethe syringe supplied to prime the pump head Attach the Luer fitting to the outlet check valve Attach the priming syringe to the outlet of the Luer fitting 3 Press the PRIME key This will run the pump at the maximum flow rate for that pump head 4 Using the syringe pull the solvent through the inlet tubing and the pump head 5 Once the pump is primed press the STOP k
16. ID for the CIM installed in the pump either by entering a number between 0 and 63 or pressing the UP ARROW or DOWN ARROW key to scroll through a preset list of choices Can be set between 0 and 63 or to no ID As Master Controller AOut Analog Out 0 10 V output signal with 8 options Output can be sent to a detector to control wavelength or to a recording device Options are Flow nm wavelength Pres sure A B C D or off PUMPS Select between A B C and D Selecting a pump opens a window to set Pump ID Head size compressibility factors and refill speed Detr Sets the ID number for any detector connected to the serial cable Bus Status Shows the status of serial devices The display shows ID numbers of devices and active devices at any of the IDs Identifiers are B C D or P for Pump M for Detector I for CIM and E for Error BUS IDs Identifies devices defined on the serial bus with their model number ID number and status online or offline device IDs of some remote devices may be changed in this window 26 218 Solvent Delivery User Manual Introduction 1 Table3 Method keys Key Function METHOD Displays method information Pressing the METHOD key opens a window where you can see the status of the running method and scroll through a list of existing methods Method Status codes are V View Indicates that the method in the display is not running Initialization The displayed meth
17. accessory kit The standard accessory kit contains the following parts p n Description 5140892700 External Contacts Panel 12 pin 2x R005400012 Double ended wrench 1 4 in and 5 16 in R007200141 Cable Recorder Integrator 6713543000 Fuse 2x 5550033300 Fuse 1 6 A 2x 5910007600 Cable 3 wire main C W plug SKT 8510249500 Voltage conversion instructions 106 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual 050 e Ove Cables e e e e d Cable Overview 108 Cable Connections 109 This chapter provides information on cables used with the instrument XE Agilent Technologies 107 9 Cables Cable Overview Necessary cables p n Description 392612901 Ethernet cable for use in a network 5023 0203 Ethernet cable cross over for standalone use 392607969 Inject marker cable 392607975 Next injection cable 393546291 Serial communication ribbon 393597601 Converter RS232 to 15422 7910046300 Serial cable Optional cables p n Description 110743800 Relay interface cable for relay interface board one relay contact per cable 110744200 Analog signal cable 108 218 Solvent Delivery User Manual Cables 9 Cable Connections Cable Connections Back connectors for 218 to internal CIM 9 Pin connectors Serial cable Converter RS 232 to RS422 GPIB Eth t cable Next t injection 1 218 Pump 7 410 Autosampler 3 325 Dete
18. as the mixer cap is hand tightened When pressurized mobile phase enters the mixer body and presses the seal against the mixer bore and the piston The increased force maintains sealing action at HPLC pressures The inlet ports on the analytical and narrowbore mixers accept standard 10 32 fittings The outlet port uses an extra long 10 32 fitting On the preparative scale both inlet and outlet ports accept 1 4 28 fittings for 0 318 cm 1 8 in tubing Effective sealing in the mixer is a function of the passive sealing mechanism only The sealing action cannot be improved by tightening the cap with more force than can be applied by hand 218 Solvent Delivery User Manual 21 1 Introduction Control Displays The left and right arrows at the sides of the display indicate that more information is off screen and can be accessed by pressing either the left arrow key or the right arrow key You can scroll left or right through the off screen information by repeatedly pressing the key Cursors In the Agilent 218 displays parameters which can be edited are indicated by one of four types of cursor All types of cursors flash both sides of the parameter to be edited Each type of cursor has a specific function Table 1 Specific cursor functions Cursor Name Function NUMERIC Used for numeric entry only Values entered or edited while the cursor is flashing are ENTRY temporary until accepted by pressing the ENTER key the RIGHT ARROW
19. disconnected Plug in power cord Power switched off Switch power on Fuse dead Replace fuse 64 218 Solvent Delivery User Manual Troubleshooting and Diagnostics 6 External Symptoms of Liquid Flow System Leaks Table 12 Leaks Probable cause Suggested actions Loose fitting s Tighten all plumbing connections no more than 1 4 turn past finger tight Worn ferrule or fitting Replace fitting and ferrule Damaged seal Replace seal Loose check valve Tighten 1 16 turn past the leak point Incorrect fitting s Reconnect with correct fittings No flow or pressure Table 13 flow or pressure Probable cause Suggested actions Pump is not operating 1 Plug in power cord 2 Switch pump on 3 Check fuses and replace if necessary Air in pump 1 Disconnect outlet fittings 2 Degas solvent 3 Divert flow to waste and pump at a high flow rate to prime pump Clogged solvent inlet filter Check and replace if necessary 218 Solvent Delivery User Manual 65 6 Troubleshooting and Diagnostics Low flow Table 14 Low flow Probable cause Suggested actions Pump is pressure limiting Reset MIN P setting to higher value Clogged solvent inlet filter Check and replace if necessary Drain valve leaking Repair leak in drain valve Excessive pressure restricted flow Table 15 Excessive pressure restricted flow Probable cause Suggested actions Tubing clogged partly clogged Crack all fittings o
20. key the LEFT CURSOR ARROW key or the RUN key If the edited value is not accepted by pressing one of the above keys or cancelled by pressing the CLEAR key the parameter reverts to its previous value after 60 seconds SCROLL Used when there is a preset list of choices Pressing the UP ARROW or the DOWN ARROW CURSOR with this type of cursor is displayed scrolls up or down through the preset choices T DUAL MODE Used when there is a preset list and numeric entry Used when the value can be set either CURSOR by numeric entry or by scrolling through a preset list of choices as described above Used when the selection is a menu Pressing the DOWN ARROW the ENTER key with CURSOR this type of cursor displays the next level of the menu The following are not cursors but are described here for clarity ASTERISK Used to show cursor position for accessing HELP when the parameter in question is a status indicator and cannot be edited lt LEFT RIGHT These indicators used at the right or left edge of the display to indicate that more ARROWS information can be seen by pressing either the RIGHT or LEFT ARROW key 22 218 Solvent Delivery User Manual Introduction 1 Keypad The keypad is functionally divided into four groups Function keys Method keys Control keys and Edit keys Table2 Function keys Key Function Flow This key opens the FLOW window where you can set flow rate and ramp time The
21. of 5 minutes 12 Press the RIGHT ARROW to get to the FLOW field and press 5 13 Press the NEW key and then press 1 0 then ENTER 14 Press the RIGHT ARROW key and then 0 ENTER 218 Solvent Delivery User Manual 43 4 Using Check and Run the Method 1 Press the LEFT ARROW key until you are at the Time field then press the UP ARROW key a number of times to read through the method time lines Press the DOWN ARROW key to scroll down through the time lines 3 Press the RUN key to begin this method You will see the time field counting down and the flow rate counting up to reach 5 mL min at time zero Then you will hear the alarm at time 0 indicating there is an inject wait If you were performing an actual run the inject wait would be cancelled by a contact closure from either the manual injection valve or an autosampler In this case cancel it as follows Press the RUN key to clear the HOLD This will act as an injection signal in this demonstration method The method will continue to run its course ramping from 0 to 5 mL min over 2 minutes and then maintaining 5 mL min until 5 minutes finally ramping down to zero flow rate at 10 minutes 44 218 Solvent Delivery User Manual 4 Using Check and Run the Method 0 0 2 2 9 rsd 2 ae psd SL 0 2 9 psd A sL 0 00 SI a 2 a 15 uru qu 05 0 00 01 2 15 uru qu os
22. pressure hazard 117 internet 126 L layout 12 liquid heads 103 lithium batteries 121 M method menu 46 method check 44 creating 43 run 44 mixer seal replacement 97 mixer 105 P parts list 102 piston seal replacement 200 mL min head 88 replacement 86 Index power cords 31 power fuses change 98 check 98 poweron 41 pressure display functions 63 pressure limits pumphead 35 pressure module overview 13 pressure modules 104 pump head priming 42 radio interference 122 replacement mixer seal 97 5 safety information lithium batteries 121 safety general information 112 sample methods 52 schedule maintenance 75 seal 200 mL min 85 servicelogs 77 site requirements power cords 31 solution 127 Index air bubble in tubing 67 baseline spikes 70 erratic pressure 67 excessive backpressure 67 excessive pressure 66 flat top peaks 69 leaks 65 low flow 66 no flow 65 no pressure 65 noisy baseline 69 68 restricted flow 66 specifications performance 34 physical 33 standard head sealremoval 81 T temperature range 33 troubleshooting guide 64 introduction 62 V voltage 33 wW washing head seal removal 83 waste electrical and electronic equipment 120 WEEE directive 120 weight 33 128 218 Solvent Delivery User Manual www agilent com In This Book The manual describes the following Introduction Site requirements Installation Using Troubleshooti
23. solvent will ooze past the fitting At this point tighten the fitting 80 218 Solvent Delivery User Manual Maintenance and Repair 7 Removing Seals Standard Head OUTLET 5ml 10ml 25ml 5ml 10mi 25mi 90mi 100mi INLET Figure 19 Standard pump head exploded view 1 Threaded cap Piston cup Piston assembly Piston guide Return spring Screws c N Bearing housing 218 Solvent Delivery User Manual 81 7 Maintenance and Repair 8 Housing spacer 9 Seal backup 10 Piston seal 11 Liquid head 12 Outlet check valve cartridge 13 Inlet check valve cartridge 14 Outlet check valve housing 15 Inlet valve housing 16 Outlet check valve cartridge 17 Inlet check valve cartridge 18 Outlet check valve housing 19 Inlet valve housing Tools required Description 3 mm wrench oc N Make sure the pump is stopped and switch off the power switch Remove the fittings at the inlet and outlet check valves Loosen the head clamp nut and remove the head clamp Pull the pump head straight out from the pump body Disassemble the pump head a Unscrew the threaded cap 1 b Remove the piston cup 2 piston assembly 3 piston guide 4 and return spring 5 c Loosen the two screws 6 at the front of the pump head Remove the bearing housing 7 and the housing spacer 8 d Remove the seal backup 9 Carefully remove the seal 10 If the seal is stuck pry it out carefully so th
24. the screw threads onto the shoulder Thread the seal retainer onto the piston and finger tighten Do not use tools OR Preparative mixers Remove the seal by pulling it away from the piston Slide the seal over the piston so the side of the seal with the larger outside diameter is closer to the bottom of the piston 5 Press the piston with seal and seal retainer on analytical and narrowbore models all the way into the mixer body bore 6 Replace the mixer cap 218 Solvent Delivery User Manual 97 7 Maintenance and Repair Checking and or Changing Power Fuses F1 When Tools required Parts required OR Preparations If the Agilent 218 Purification Solution does not operate when the power cord is connected and the power switch is on the fuse s may need replacing Fuses are located in the power module on the back panel Description Flat hat screwdriver Description 1 15 AH 250 V 115 V option 1 13 15 AH 250 V 220 230 V option Check following probable causes for the power fail before starting exchanging the fuses Power cord properly connected Power at the wall receptacle Electrical shock Always disconnect power cord from the module before replacing a fuse CAUTION Wrong fuse Damage to the module and loss of warranty Set voltage properly Use only the correct fuses 98 218 Solvent Delivery User Manual Maintenance and Repair 7 Checking and or Changing Power Fuses F1
25. to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 218 Solvent Delivery User Manual In This Book In This Book This manual contains information on e G9300A Agilent 218 Isocratic Solvent Delivery Module e G9301A Agilent 218 Add on Solvent Delivery Module G9306A Agilent 218 Injection Pump 1 Introduction This chapter gives an instrument overview 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installation This chapter gives information about the installation of your instrument 4 Using This chapter explains the operational parameters of the instrument 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features 218 Solvent Delivery User Manual 3 In This Book 7 Maintenance and Repair This chapter describes the
26. 0 mL min Head Preparations The necessary seal tools come as part of the pump head kit Piston seal and seal location are clean undamaged and completely free from foreign matter 1 Before installing a new piston seal make sure you are installing the piston seal and not the washing section seal Piston seal 7 The piston seal has ridges on its edge Washing section seal 2 Putthe seal insertion tool with protruding ring down 3 Place the piston seal spring side down in the seal into liquid head and make sure it is flush against the liquid insertion tool with the spring side of the seal down head 88 218 Solvent Delivery User Manual Maintenance and Repair 7 Replacing Piston Seals 200 mL min Head 4 Place the seal plunger tool on top of the seal and press the seal into place 5 Remove seal insertion tools and reassemble the pump head following Figure 20 on page 83 and the ink mark which was placed on the pump head body before disassembly see Removing seals 200 mL min Head on page 85 8 Before use break in the seal using a 100 methanol 218 Solvent Delivery User Manual 7 Maintenance and Repair Breaking In a New Seal 200 mL min Head When After seal replacement 1 Install a 60 90 cm 2 3 ft length of 0 25 mm 0 010 in ID PEEK or stainless steel tubing downstream from the pump which will create backpressure
27. Agilent 218 Solvent Delivery Module User Manual Agilent Technologies Notices Agilent Technologies Inc 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G9300 90000 Edition 09 2012 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Ag
28. EEK FEP 28 28 4000 50 100 mL min PEEK FEP 28 28 2000 100 200 mL min Titanium FEP 28 28 400 200 1 Uses 0 318 cm 1 8 in ID tubing 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual s i 38 i e Installation ee e eo 92 90 e This chapter gives information about the installation of your instrument Agilent Technologies 37 3 Installation Installation For details on installation of the module refer to Agilent 218 Purification System Setup and Installation Guide p n G9300 90300 38 218 Solvent Delivery User Manual This chapter explains the operational parameters of the instrument 218 Solvent Delivery User Manual 4 Using System Overview 40 PowerOn 41 Priming the Pump Heads 42 Creating a Simple Method on an Agilent 218 Pump 43 Check and Run the Method 44 Method Menu 46 Sample Methods 52 RE Agilent Technologies 39 4 Using System Overview 40 The Agilent 218 Purification Solution can be used in several different modes of operation including operation as a master pump in an automated HPLC system This section describes priming the pump and method editing with an Agilent 218 Purification Solution as a master pump For operation information using the control software how to configure the driver and create a method see help of control software or Agilent 218 Purification System Setup and Installation
29. HOLD point HOLD Stops the method timer but does not change current flow or composition conditions Holds the ramp at its current position This key is cancelled by pressing RUN again or STOP PRIME Runs the pump at the maximum flow rate for the installed pump head Pressing STOP stops the pump when itis priming All other keys are locked out from operation while the pump is priming STOP Stops flow immediately and aborts the Run method Table5 Edit keys Key Function 0 9 and Numeric keys used to enter numeric values into parameters method number flow rate minimum and maximum pressure limits pump ID CIM ID etc CLEAR Used to cancel a user entered value or choice leaving the previously entered setting intact Also used when zeroing pressure answers No to prompts and clears messages U D Arrows Used to scroll through a list of options and select an item from the list ENTER Accepts a temporary value into a parameter Answers Yes to prompts 28 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual ee 2 Site Requirements and Specifications o o E e e e Site Requirements 30 Physical Specifications 33 Performance Specifications 34 This chapter provides information on environmental requirements physical and performance specifications XE Agilent Technologies 29 2 Site Requirements and Specifications Site Requirements Power Considerations The instrument power supply has wide ranging
30. age of the electronic components or personal injury Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 218 Solvent Delivery User Manual 31 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 6 on page 33 allow you to place the module on almost any desk or laboratory bench It needs an additional 5 2 in of space on either side and approximately 15 5 9 in at the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 32 218 Solvent Delivery User Manual Physical Specific
31. at the liquid head 11 is not scratched Do not reuse a seal after it has been removed 82 218 Solvent Delivery User Manual Maintenance and Repair 7 Removing Seals Washing Head 200 mL min Two identical piston seals are incorporated into each washing head one in the head body and one in the washing section Each piston seal consists of a seal ring made from high density polyethylene HDPE and a spring made of Hastelloy C276 Both piston seals should be replaced at the same time OUTLET Figure 20 Washing head exploded view 1 Threaded cap 2 Piston cup 3 Piston assembly 4 Piston guide 5 Return spring Bearing housing 7 Housing spacer 8 Washing section 9 Seal backup 10 Piston seal 218 Solvent Delivery User Manual 83 7 Maintenance and Repair 11 Liquid head 12 O ring 13 Seal backup 14 Piston seal 15 Inlet check valve cartridge 16 Inlet check valve support screw 17 Screw 18 Outlet check valve cartridge 19 Outlet check valve support screw Tools required Description 3 mm wrench Make sure the pump is stopped and switch off the power switch Remove the fittings at the inlet and outlet check valves Loosen the head clamp nut and remove the head clamp Pull the pump head straight out from the pump body A oc N Disassemble the pump head a Unscrew the threaded cap 1 b Remove the piston cup 2 piston assembly 3 piston guide 4 and return spring 5
32. atically at the end of the method if no transfer signal is received If you do not wish the method to transfer leave this entry blank 4 Use the UP ARROW or DOWN ARROW key to scroll between existing method numbers OR Enter the method number with the number keys 5 Under the MODE field use the UP ARROW or DOWN ARROW key to select between IMMED iate or DEFER ed fyou choose IMMED the transfer function will occur immediately on receipt of the transfer contact closure If you choose DEFER the transfer will take place at the end of the current method pass IF a transfer contact closure is received e fthere is no transfer contact closure received the method will transfer automatically at the end of all method passes 218 Solvent Delivery User Manual 49 4 Using 6 Press the Method key to leave this sub menu and return to the Method Menu Assign a SAFETY Meth Set the method to be transferred to in the event of an emergency condition or contact closure to the Agilent 218 Purification Solution 1 Use the LEFT ARROW or RIGHT ARROW key to move the cursor on SAFETY METH 2 Press the DOWN ARROW key Meth STOP HIGH P LOW P OFF LINE vo 3 Select between the available methods for each field with the UP ARROW or DOWN ARROW key OR Enter the method number with the number keys Table 10 Safety methods Setting Use case STOP Used to set the method to be used in the event of a STOP signal to the
33. ations Site Requirements and Specifications 2 Table 6 Specifications Agilent 218 Purification Solution Type Specification Rated voltage 100 240 VAC 90 264 Absolute 50 60 Hz The Agilent 218 Purification Solution may be wired for either 115V 10 50 60 Hz single phase 230V 10 50 60 Hz single phase Weight 23 6 kg 52 0 Ib Dimensions height x 197 x 292 x 464 mm 7 8 x 11 5 x 18 3 in width x depth Line voltage 115 230 V 10 Line frequency Power consumption Ambient operating temperature Humidity Operating altitude 50 60 Hz 550 VA 3 40 20 80 up to 2000 m The 218 System Pump is suitable for indoor use only and is classified Pollution degree 2 and Installation Category Il EN 61010 1 1 All power supplies should be single phase AC 3 wire system active neutral ground and should be terminated at an appropriate connection receptacle that is within reach of the system power ca ble 218 Solvent Delivery User Manual 33 2 Site Requirements and Specifications Performance Specifications 34 Table7 Performance specifications Agilent 218 Purification Solution Type Specification Display Backlit LCD with 2 lines 48 characters Programs Up to 100 methods with unlimited timed events Interface Digital serial input output channel RS 422 Flow Accuracy Flow Reproducibility Connections Fluid Path 3 programmable contact closur
34. capability It accepts any line voltage in the range described in Physical Specifications Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified Connect your instrument to the specified line voltage only CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged Provide sufficient space behind the power socket of the instrument to unplug the cable 30 218 Solvent Delivery User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit Never operate your instrumentation from a power outlet that has no ground connection Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to dam
35. ch was last viewed Pressing the 1 0 key a second time returns to the default position with the cursor on nm 24 218 Solvent Delivery User Manual Introduction 1 Table 2 Function keys Key Function PRESSURE Opens the PRESSURE window containing several pressure related items ZERO The pressure can be zeroed using the up down arrow key Executing a Zero command displays a prompt When system pressure is more than 50 psi a second prompt with current pressure is displayed psi bar MP Current system pressure is shown in the selected units a MAX P Maximum system pressure limit in the units selected MIN Minimum system pressure limit in the units selected UNITS Select between psi bar and MPa mega Pascals OUTPUT Set the output contacts to be used for MAX P and MIN P signals CONTACTS The same contact can be set for both high limit and low limit 218 Solvent Delivery User Manual 25 1 Introduction Table2 Function keys Key Function SETUP Opens the Set Up and Service Log menus The Service Log is used to log piston seal changes check valve changes service intervals and show the pump drive status See the Maintenance section for details of this function The Set Up menu appears in one of two forms depending on whether the Agilent 218 is a master controller or a slave pump In both cases Set Up is used to set up various parameters listed below As Slave Pump ID Pump ID Set the ID for the
36. ctor Inject marker cable 440 Fraction o i 5 CPU Figure25 Cable connections for workstation control of Agilent 218 Pumps Agilent 325 Detector Agilent 410 Autosampler and Agilent 440 Fraction Collector 218 Solvent Delivery User Manual 109 9 Cables Cable Connections 110 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual 10 Appendix General Safety Information 112 Solvent Miscibility 118 Solvent Compressibility 119 The Waste Electrical and Electronic Equipment Directive 120 Batteries Information 121 Radio Interference 122 CE Compliance 123 Electromagnetic Compatibility 124 Agilent Technologies on Internet 126 This chapter provides addition information on safety legal and web RE Agilent Technologies 111 10 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired The operator of this instrument is advised to use the equipment i
37. d OR Isopropanol not miscible with solven in the pump head 1 Pump an intermediate solvent in the pump head see Solvent Miscibility on page 118 2 Pump isopropanol in the pump head 4 With the pump head filled with isopropanol stop the flow for approximately 15 min to dissolve deposits 5 Flush the isopropanol from the head and return to operating conditions Using an intermediate solvent if necessary 92 218 Solvent Delivery User Manual Maintenance and Repair 7 Cleaning Check Valves with 20 6 Nitric Acid If the above cleaning procedure does not restore normal performance you can try using 20 nitric acid to dissolve the deposits Chemical burns to eye skin and respiratory tract A 20 nitric acid solution is a strong acid Strong acids are extremely corrosive and pose severe risks gt Observe all standard laboratory safety precautions when using strong acids Always wear appropriate personal protective equipment 1 Soak check valve in 20 nitric acid for ten minutes 2 Carefully remove the check valve from the acid bath and rinse thoroughly with deionized water 3 Reinstall the check valve on the pump head If this procedure fails to correct the problem you should replace the check valve as described in Replacing Check Valves on page 94 218 Solvent Delivery User Manual 93 7 Maintenance and Repair Replacing Check Valves Check valve failure may be indicated by a severe loss in flow ra
38. e Check ball Figure 6 Outlet check valve sectional view 218 Solvent Delivery User Manual Introduction 1 17 1 18 Introduction Inlet check valves are similar to outlet check valves but are installed on the liquid head the other way up Both types of valve the check valve cartridge assembly is oriented as shown Principle of Operation The retracting piston creates a negative pressure in the piston chamber above the inlet check valve Mobile phase flows upward past the check ball into the inlet check valve then into the piston chamber As soon as the piston starts to move forward gravity causes the ball in the inlet check valve to seat preventing mobile phase from flowing back out the inlet check valve At the same time a positive pressure is created in the piston chamber which dislodges the outlet check valve check ball Mobile phase flows upward through the outlet check valve while the piston is moving forward When the piston retracts again gravity causes the ball in the outlet check valve to seat preventing mobile phase flowing back out the outlet check valve and the cycle is repeated During manufacture each check valve is closely inspected and then individually assembled in a clean room Check valves should be kept clean and in good condition for reliable reproducible flow 218 Solvent Delivery User Manual Introduction 1 Dual Chamber High Pressure Mixer Mixers are dual chamber high p
39. e relay outputs b5contact closure inputs 1 analog input 0 5 V to 2 5 V 18 bit A D converter 1 programmable analog output 0 10 V 1 of selected flow rate or 0 05 of maximum flow whichever is larger 0 1 96 for 5 mL min heads 0 1 of selected flow or 0 05 of maximum flow whichever is larger 0 01 96 for 5 mL min heads 1 4 28 inlet flanged or gripper type fitting 1 4 28 outlet for nut and ferrule 316 stainless steel titanium sapphire or ceramic ruby PCTFE PTFE or HDPE 218 Solvent Delivery User Manual Site Requirements and Specifications 2 Table8 Pressure limits pump heads Nominal flow Range SST and Titanium PEEK pressure mL min mL min pressure maximum maximum 10 0 01 10 8700 psi 4000 psi 600 bar 215 bar 60 0 MPa 27 6 MPa 25 0 025 25 6000 psi 4000 psi 414 bar 215 bar 41 4 MPa 27 6 MPa 100 0 1 100 4000 psi 2000 psi 215 bar 137 bar 27 6 MPa 13 8 MPa 200 0 2 200 3500 psi N A 241 bar 24 1 MPa 218 Solvent Delivery User Manual 35 2 36 Site Requirements and Specifications Table9 Performance specifications pressure modules Description wetted Inlet fittings Outlet fittings pressure flow rate materials in in psi mL min 10 mL min Titanium FEP 28 7 28 8700 10 50 mL min Titanium 7 28 7 28 6000 50 100 mL min Titanium FEP 7 28 7 28 1200 100 10 mL min PEEK 28 28 4000 10 50 mL min P
40. er controller or a slave pump The possible system configurations depending on the type of pumps and controller being used are the following socratic system Gradient system with one Agilent 218 Pump as the controller When several pumps are connected together the master Agilent 218 Pump can control the other pumps in the liquid delivery system A master Agilent 218 Pump can control up to three other slave units either three additional pumps in a quaternary system or two additional elution pumps and one injection pump The master Agilent 218 Pump can control other modules in the system using outputs and receive information through input contacts Gradient system with HPLC control software as a controller In this configuration all pumps are slaves and the computer is the system controller The HPLC control software controls the pumps via the serial interface cable and other devices through contact closures on the Control Interface module CIM built into the Agilent 218 Pump 218 Solvent Delivery User Manual 11 1 Introduction Physical Layout Physical Layout Figure 1 Agilent 218 Solvent Delivery Module front panel Figure2 Agilent 218 Solvent Delivery Module rear panel 12 218 Solvent Delivery User Manual Introduction 1 Pressure Module Each Agilent 218 Pump can have a pressure module installed in the panel on the right side of the pump PRESSURE MODULE 8700 psi 10 n
41. evision are on separate electrical circuits Make sure that all peripheral devices are also certified Make sure that appropriate cables are used to connect the device to peripheral equipment Consult your equipment dealer Agilent Technologies or an experienced technician for assistance Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment 218 Solvent Delivery User Manual Appendix 10 ICES NMB 001 This ISM device complies with Canadian ICES 001 Cet appareil ISM est conforme la norme NMB 001 du Canada 218 Solvent Delivery User Manual 125 10 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 126 218 Solvent Delivery User Manual Index 2 200 mL min head seal removal 85 A accessory package 106 Agilent oninternet 126 air bubbles clearing 80 battery safety information 121 bench space 32 C check valve overview 1 7 check valves cleaning 92 compliance 123 condensation 32 connections workstation control 109 D dimension 33 dual chamber 19 E electronic waste 120 218 Solvent Delivery User Manual F flow rate adjusting 79 high pressure mixer 19 high
42. ey to stop the pump Remove the Luer fitting from the outlet check valve and replace it with the outlet tubing 218 Solvent Delivery User Manual Using 4 Creating a Simple Method on an Agilent 218 Pump The following method will ramp an Agilent 218 Pump to 5 mL min in 2 minutes to time 0 00 At time 0 00 the pump will wait for inject and Alarm make the injection and maintain flow rate at 5 mL min for 5 minutes After 5 minutes the flow rate will ramp to 0 mL min over 5 minutes Use a pump head size of 10 mL or greater for this method 1 Press the METHOD key 2 Press the NEW key a new method number will be selected and displayed automatically The method number displayed will be the next available number from 0 to 99 3 Press the FLOW key 4 The starting time is shown in minutes default is 2 00 If this is not displayed press 2 ENTER This is the time for the linear ramp to initial conditions 5 Press the DOWN ARROW key to get to time 0 00 6 Press the RIGHT ARROW key to move to the FLOW field Press 5 flow rate of 5 mL min 7 Press the 1 0 key then the RIGHT ARROW key to get to the w field Press the UP ARROW key with the cursor on the w field The value changes to 1 ON 8 Press the RIGHT ARROW key to get to the A field Press the DOWN ARROW key The value changes to P Pulse on then off 9 Press the FLOW key then the LEFT ARROW key to get to the Time field 10 Press the NEW key 11 Press 5 to set a time
43. f line RECALL Returns the pump to the current status timeline of the running method The fields displayed in this view are the same as were displayed the last time the FLOW or 1 0 key was pressed in the running method When on the current time line RECALL returns to the last display viewed 218 Solvent Delivery User Manual 27 1 Introduction Table3 Method keys Key Function HELP Opens a Help display for the parameter which is selected In most cases the help information is longer than will fit a two line display Scroll through the help message by pressing the HELP key the RIGHT ARROW key or the DOWN ARROW key Scroll back through the Help message by pressing the UP ARROW or the LEFT ARROW key Press the CLEAR key or the HELP key at the end of the message to exit the Help L R Arrows These keys are used to move right and left in the display to access adjacent menus and to set values U D Arrows These keys are used to scroll up or down through preset values or toggle between choices They are also used to select time lines in a method The Down Arrow key is also used to open menus ENTER The Enter key is used to open menus It also is used to accept a value Table4 Control Keys Key Function RUN Starts the method timer and begins a linear ramp from current conditions to the target conditions at the next time line in the method If used to clear a Hold see below RUN continues the run from the
44. grammed yet 2 Press the NEW key New Meth COPY OF Meth 55 TIMES ACCESS TRANSFER SAFETY MODE 0 2 UNLOCK Meth Meth NORMAL Creating a New Method The cursors around the method number 0 in this case will be flashing This means that you can select a method number for your new method 1 Press the desired number key s OR Press the UP ARROW or DOWN ARROW key to scroll through the available unused method numbers OR Access the default number The first time you do this all methods from 0 99 will be selectable However if you have methods already created you will not be able to select or enter allocated method numbers If you enter a number with the keys that is already used you will get the message INVALID METH xx EXISTS 218 Solvent Delivery User Manual 47 4 48 Using You can create up to 99 methods This may seem like overkill but is useful when there are multiple users in a lab Specialized methods such as Transfer methods or Safety methods could be allocated from 90 99 Examples on how to use this feature Analyst A could use methods from 20 29 and Analyst B from 40 49 Specialized methods such as Transfer methods or Safety methods could be allocated from 90 99 Copying Methods The Agilent 218 Purification Solution allows you to duplicate an existing method This is useful if you want to create a new method with the same or similar conditions as an existing method Perhap
45. ibrating parts Wrap tubing column Remove or cover heat or cooling source 68 218 Solvent Delivery User Manual Troubleshooting and Diagnostics 6 Drifting baseline Table 20 Drifting baseline Probable cause Suggested actions Contaminated flow cell Attach a syringe to the flow cell inlet and try to clear blockage by drawing on the syringe Or attach to outlet and back flush to clear blockage by gentle pressure on the syringe Do not apply pressure to the flow cell inlet Localized temperature effects Wrap tubing column Remove or cover heat or cooling source Contamination in column Wash or replace column Change mobile phase Leak in system Locate leak and repair Bubble trapped in flow cell Flush flow cell Degas solvent Add back pressure device to flow cell Column not equilibrated Flush system until column is equilibrated Mobile phase contamination Use fresh HPLC grade solvents Weak detector lamp Replace detector lamp Flat top peaks Table21 Flat top peaks Probable cause Suggested actions Saturated electronics Reduce sample volume Recorder adjusted incorrectly Set recorder correctly Bad grounding Check all grounding connections on pump and ensure grounded AC power is supplied to all devices in HPLC system 218 Solvent Delivery User Manual 69 6 70 Troubleshooting and Diagnostics Baseline spikes Table 22 Baseline spikes Probable cause Sugges
46. ilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered
47. ions Clogged mixer frit Stir bar spacer sticking Blocked tubing Damaged ferrule in compression fitting Replace frit Dismantle and clean mixer Filter solvents Check solvent miscibility Loosen fitting after each component to find blockage Replace affected tubing Replace ferrule Do not over tighten 1 218 Solvent Delivery User Manual Analytical and narrowbore mixers only 67 6 Troubleshooting and Diagnostics Detection Symptoms Noisy baseline Table 19 Noisy baseline Probable cause Suggested actions Air bubbles through flow cell Leak in system plumbing Contaminated flow cell Detector lamp failing Bad grounding Electronic interference Localized temperature effects Install backpressure regulator Divert flow to waste and pump at a moderately high flow rate to prime pump Check tubing fittings Degas solvent Check for deposits around fittings and check that all fittings are tight Attach a syringe to the flow cell inlet and try to clear blockage by drawing on the syringe Or attach to outlet and back flush to clear blockage by gentle pressure on the syringe Do not apply pressure to the flow cell inlet Check and replace if necessary Check all grounding connections on pump and ensure grounded AC power is supplied to all devices in HPLC system Check for loose connections Ensure instruments are not in direct contact with each other or with v
48. itable detector when the analog out signal has been set to the nm option in Setup or there is a detector online Range is 1 90 V 190 nm to 7 00 V 700 nm w Inject Wait When a method is running this is used to set an inject wait The method will hold at current conditions until the wait is cancelled when the method will continue The wait can be cancelled manually by pressing the RUN key or by contact closure on the Inject input A Alarm Can be set to 1 on 0 off or P Pulse on then off If Pulsed the alarm will sound three times then off If set to Pulse at the same time as an inject wait the alarm sounds four times then off Otherwise the alarm sounds continuously until turned off 1 12 Output contacts Can be set to 0 off P Pulse on then off 1 on or P Pulse off then on Pulses last for 0 5 s Contacts 1 3 are for Pump A or the Master Pump in a multi pump configuration The Master Pump can control the outputs as follows Contacts 1 2 are for Pump or the Master Pump Contacts 4 5 6 are for Pump B Contacts 7 8 9 are for Pump C Contacts 10 11 12 are for Pump D One or two contacts can be dedicated for High and Low Pressure signals defined in the pressure window below Pressure States are H for High L for Low or b for both if the same contact is used for high and low limits Pressing the 1 0 key when in another display returns the display to the part of the 1 0 field whi
49. ivery User Manual 7 Maintenance and Repair Replacing Piston Seals Heads 200 mL min Preparations The necessary seal tools come as part of the pump head kit Piston seal and seal location are clean undamaged and completely free from foreign matter 1 Place the guide on the head or rinse body with the beveled side facing out 2 Insert the new seal into the guide with spring down 3 10 and 25 mL min heads Press the seal into place using the seal tool SEAL TOOL SEAL BEVELLED EDGE OUT SPRING SIDE IN Figure 21 Seal replacement for 10 and 25 mL min heads OR 100 mL min head Press the seal into place using the seal tool SEAL GUIDE SEAL TOOL SPRING SIDE IN BEVELLED EDGE OUT Figure 22 Seal replacement for 100 mL min heads 86 218 Solvent Delivery User Manual Maintenance and Repair 7 4 Reassemble the head by reversing the directions for seal removal noting the following Align the ball housing assembly the housing spacer the rinse body for washing heads and the head body so the matching hole or notch is on the same side on each component Thread the ball housing assembly the housing spacer and the rinse body for washing heads to the head body After these parts are attached put the assembly on a flat surface to facilitate mounting the other parts 218 Solvent Delivery User Manual 87 7 Maintenance and Repair Replacing Piston Seals 200 mL min Head Replacing Piston Seals 20
50. ized open ended wrench CAUTION Damage to the liquid head and the check valve gt Do not overtighten the check valve into the liquid head 5 Run the pump and inspect for leaks around each check valve If leaks are present tighten the check valve only enough to stop the leak Check for leaks again tightening only if necessary 218 Solvent Delivery User Manual 95 7 Maintenance and Repair Checking and Replacing the Mixer Outlet Filter Frit Analytical and Narrowbore Mixers Only 96 The analytical and narrowbore mixers incorporate a 2 um frit pressed into the seal retainer to protect the downstream components of the HPLC system from particulate contamination With normal use this frit may become clogged resulting in restricted flow through the mixer and HPLC system To check for restricted flow you will need a pressure module in the HPLC system between the pumps and the mixer Check the flow 1 2 Open the prime purge valve so the flow is diverted to waste Run the pumps with methanol rinsing first with water if necessary at a moderate flow rate 1 mL min and check the pressure monitor If the frit and the fluid lines are in good condition the pressure reading on the monitor should be minimal A reading over 100 psi 6 7 bar 0 67 MPa indicates that the frit is probably clogged and needs to be replaced A clogged frit cannot be cleaned Replace the frit The frit can be replaced without fully disman
51. losion proof Certain solvents may cause weakening and leaks of tubings or fitthings with possible bursting Even small leaks in solvent supply systems can be dangerous Only use solvents compatible with the HPLC system tubings and fittings Employ static measuring and static discharge devices to safeguard against the buildup of static electricity n unattended operation do not use organic solvents having an ignition point below 70 C Do not bring a heat or flame source near the instrument The area in which solvents are stored and the area surrounding the instrument must be adequately ventilated to prevent accumulations of gas Always check the condition of the instrument leakage of solvent or waste solution leakage of solvent inside the instrument If an abnormality is found stop operation immediately When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste Use only approved regulator and hose connectors refer to the supplier s instructions Keep solvents cool and properly labeled Ensure that you have the correct solvent before connecting it to the instrument 218 Solvent Delivery User Manual 115 10 Appendix WARNING Inflammation or injury due to toxic corrosive or stimulative solvent Do not contact toxic corrosive or stimulative solvent For details of the
52. maintenance of the instrument 8 Parts This chapter provides information on parts for the instrument 9 Cables This chapter provides information on cables used with the instrument 10 Appendix This chapter provides addition information on safety legal and web 4 218 Solvent Delivery User Manual Contents Contents 1 Introduction 9 Introduction to the System 10 Physical Layout 12 Pressure Module 13 Pump Head 15 Dual Chamber High Pressure Mixer 19 Control 22 2 Site Requirements and Specifications 29 Site Requirements 30 Physical Specifications 33 Performance Specifications 34 3 Installation 37 Installation 38 4 Using 39 System Overview 40 PowerOn 41 Priming the Pump Heads 42 Creating a Simple Method on an Agilent 218 Pump 43 Check and Run the Method 44 Method Menu 46 Sample Methods 52 5 Optimizing Performance 59 Choose the Appropriate Pump Head for the Application 60 218 Solvent Delivery User Manual Contents 6 Troubleshooting and Diagnostics 61 Introduction to Troubleshooting and Diagnostics 62 Using the Pressure Display as Diagnostic Tool 63 Troubleshooting Guide 64 Maintenance and Repair 71 Introduction to Maintenance 72 Warnings and Cautions 73 Maintenance Schedule 75 Service Logs 77 Adjusting the Flow Rate onthe Pump 79 Clearing Air Bubbles from the Liquid Head 80 Removing Seals Standard Head 81 Removing Seals Washing Head 200 mL min 83 Removing seals 200 mL min Head 85
53. mast kit detector and optional fraction collector or autosampler This manual is set up to help guide you through an Agilent 218 Solvent Delivery Module installation comprising pressure module mixer if ordered mast kit and tubing The Agilent 218 Pump uses proven single piston rapid refill technology for economy reliability and virtually pulse free operation range of interchangeable pump heads allows operation at flow rates from 10 uL min to 200 mL min Biocompatible pump heads are available for those analysts requiring a completely inert flow path A single channel analog to digital converter built in to each Agilent 218 Pump can convert a detector signal to digital form and transmit the data to a computer system Five programmable analog inputs and three programmable relay outputs are available to further automate the HPLC system The Agilent 218 Pump is easy to use and very flexible in operation It can be used in several different modes of operation as a standalone isocratic pump as either a master pump or a slave pump in a high pressure gradient system as a sample inject pump in a preparative system or in a fully automated HPLC system controlled by an external computer In each case the Agilent 218 Pump provides outstanding accuracy over its entire range of pressures flow rates and solvents The Agilent 218 Pump operates very quietly because of minimal motor noise and resonance vibrations complete Agilent 218 Pump i
54. mp The clamp on the 218 pump with 200 mL min head requires a 1 4 in hex wrench that is included with the 200 mL min pump head kit 218 Solvent Delivery User Manual 15 1 16 Introduction The following pump heads are available p n R007101061 R007101062 R007101063 R007101073 393594291 R007101064 R007101074 R007101076 R007101077 393650701 Description Pump head stainless steel 10 mL min Pump head stainless steel 10 mL min with piston wash Pump head titanium 10 mL min with piston wash Pump head PEEK 10 mL min with piston wash Pump head titanium 25 mL min with piston wash Pump head stainless steel 25 mL min Pump head PEEK 25 mL min with piston wash Pump head PEEK 100 mL min with piston wash Pump head titanium 100 mL min with piston wash Pump head kit 218 ONLY titanium 200 mL min These heads incorporate a second chamber located behind the high pressure seal This chamber filled with water literally washes the piston with each stroke This prevents scale build up on the piston that can lead to premature seal failure 218 Solvent Delivery User Manual Check Valves The pump head in the Agilent 218 Pump has one inlet check valve and one outlet check valve Outlet check valve Pump body Piston Liquid head Inlet check valve Figure 5 Washing head cutaway view Check valve housing Washer and check ball retainer Check valve cartridg
55. n a manner as specified in this manual 112 218 Solvent Delivery User Manual Appendix 10 Information Symbols The following is a list of symbols that appear with warnings in this manual or on the liquid chromatograph The hazard they describe is also shown triangular symbol indicates a warning The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows Table 27 Warning symbols gt gt gt Broken glass Chemical Electrical hazard shock Explosion Eye hazard Fire hazard hazard gt gt gt Heavy weight Heavyweight Hot surface danger to danger to E D Xt D 5 a u Moving parts Respiratory Attention hazard 1 The symbol may be used on warning labels attached to the instrument When you see this symbol refer to the relevant operation or service manual for the correct procedure re ferred to by that warning label 218 Solvent Delivery User Manual 113 10 Appendix Table 28 Information symbols Symbol Description Mains power on Mains power off Fuse FU AAA Single phase alternating current Direct current When attached to the rear of the instrument indicates that the product complies with the requirements of one or more EU directives 114 218 Solvent Delivery User Manual Appendix 10 Solvent Hazards WARNING Explosion fire asphyxiation This instrument is not exp
56. n geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 218 Solvent Delivery User Manual 121 10 Appendix Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 122 218 Solvent Delivery User Manual Appendix 10 CE Compliance Your instrument has been designed to comply with the requirements of the Electromagnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Agilent has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with these directives is indicated by the CE Marking appearing on the rear of the product and the documentation package that accompanies the product containing a copy of the Declaration of Conformity The Declaration of Conformity is the legal declaration by Agilent that the product complies with the directives listed above and shows the EN standards to which the product was tested
57. n seals check valves piston assembly seal back up return spring Continuous unattended operation is common with HPLC components A general guideline for the replacement frequency of consumable components is indicated in the table below The table assumes the pump is working at half its maximum flow rate and pressure Replacement frequency is indicated for intensive regular and occasional pump use The solvent used also effects replacement frequency Mobile phases with buffers and high ionic strength will cause the seals to fail more frequently Table 23 Frequency of component replacement Component Use Intensive 168 hrs wk Regular 40 hrs wk Occasional 10 hrs wk Piston seal 4 6 months 1 year 2 years Check valves 6 12 months 2 years 4 years Piston assembly 1 2 years 4 6 years 8 10 years Seal back up 1 2 years 4 6 years 8 10 years Return spring 2 years 4 6 years 8 10 years This table is only a guideline pump head components may need to be replaced more or less often than indicated Changes in performance or visible leaks give more precise indication that a part should be replaced Seals need to be replaced every so often This is because friction from the moving pistons eventually abrades the seal and pressurized liquid in the piston chamber seeps past the seal Seal wear may be indicated by leaks from 218 Solvent Delivery User Manual 75 7 Maintenance and Repair the notch at the bottom of
58. ncludes a drive module a pump head and pressure module One of the Agilent 218 Pumps in the HPLC system needs to have a pressure module installed in its compartment in the pump side panel The pressure module dampens pulsations and supplies the current system pressure value to the drive module Software in the drive module ensures that the system pressure is within pre set maximum and minimum limits Flow rates are automatically corrected for solvent compression effects based on the system pressure value read from the pressure module and a compressibility factor entered by the user for each solvent 218 Solvent Delivery User Manual Introduction 1 The Agilent 218 Pump operates with a variety of 218 pump heads to maintain specified performance over designated flow and pressure ranges The easily replaceable pump heads are self contained units including a spring loaded piston and check valve cartridges Pump heads are not included with individual drive modules A complete HPLC system can be controlled either by an Agilent 218 Pump or PC based software When the computer controls pumps all pumps are slaves and programming is done on the computer On the pump rear panel there is a single RS 422 male connector This connector is used for bidirectional signals to and from the controller whether the controller is an external computer or another Agilent 218 Pump Internal software in the Agilent 218 Pump determines whether the pump is a mast
59. ne by one until the pressure reverts to normal Then replace the section of tubing immediately after the last cracked fitting Injection valve clogged Flush injection valve replace sample loop If this does not clear the blockage see the injection valve manual Injector between LOAD INJECT Reposition to LOAD or INJECT Frit filter in column clogged Replace the column frit Detector flow cell clogged Attach a syringe to the flow cell inlet and try to clear blockage by drawing on the syringe Or attach to outlet and back flush to clear blockage by gentle pressure on the syringe Do not apply pressure to the flow cell inlet 66 218 Solvent Delivery User Manual Erratic pressure Table 16 Erratic pressure Troubleshooting and Diagnostics 6 Probable cause Suggested actions Leak Check and repair leaks Air in pump 1 Disconnect outlet fittings 2 Degas solvent 3 Divert flow to waste and pump at a moderately high flow rate to prime pump Air bubble in tubing Table 17 Air bubble in tubing Probable cause Suggested actions Loose inlet tubing connection Worn flange in inlet tubing Loose inlet check valve Inlet filter partially clogged Loose outlet tubing connection Tighten inlet fittings Remake inlet tubing flange Tighten 1 16 turn past the leak point Clean or replace Tighten outlet fittings Excessive backpressure Table 18 Excessive backpressure Probable cause Suggested act
60. ng and error information Maintenance and repair Parts Safety Agilent Technologies 2012 Printed in Germany 09 2012 G9300 90000 AES Agilent Technologies
61. nt Applications Choosing the best pump heads for gradient applications is slightly different If possible choose the pump head so that the system operates at or above 10 of the minimum pump head flow rate Also try to use the smallest pump head possible for gradient operation Protein Purification Applications Biocompatible pump heads constructed entirely of titanium or chemically inert plastics are available These heads incorporate a second chamber located behind the high pressure seal This chamber filled with water literally washes the piston with each stroke This prevents scale build up on the piston that can lead to premature seal failure 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual 6 Troubleshooting and Diagnostics o o E e e a 7 Introduction to Troubleshooting and Diagnostics 62 Using the Pressure Display as Diagnostic Tool 63 Troubleshooting Guide 64 This chapter gives an overview about the troubleshooting and diagnostic features RE Agilent Technologies 61 6 Troubleshooting and Diagnostics Introduction to Troubleshooting and Diagnostics Troubleshooting an HPLC system requires a methodical approach to be effective To correct a given problem proceed step by step eliminating each variable in turn before moving to the next Some problems have more than one cause and can be difficult to locate and correct The troubleshooting guide lists some common pum
62. o the minimum required for the analysis Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened Do not remove the cover of the module Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations Use your Agilent products only in the manner described in the Agilent product user guides 218 Solvent Delivery User Manual 73 7 Maintenance and Repair Warnings and Cautions CAUTION Safety standards for external equipment 2 f you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 74 218 Solvent Delivery User Manual Maintenance and Repair 7 Maintenance Schedule User maintenance on the Agilent 218 Pump is generally limited to the pump head as follows Cleaning the check valves and filter Replacing components subject to wear and tear pisto
63. od is ramping to time 0 inject R Running The displayed method is running beyond time 0 S Stop The displayed method is stopped and flow rate 0 by the STOP key or external input or by the method finishing with 0 mL min flow rate H Hold The displayed method is holding Either the HOLD key was pressed an Inject Wait programmed or an external input was received F Finished The method has run and finished normally with flow rate 0 For non manual methods the Agilent 218 displays a list of method parameters which can be accessed by scrolling with the RIGHT ARROW and LEFT ARROW keys Meth This shows the Method name and status see above PASS Indicates the current method pass if there are more than one TIMES Indicates the total number of times up to 99 that the method will be executed before it finishes Access method will be executed before it finishes Access User can scroll between LOCK and UNLOCK Locked methods cannot be edited They are saved intact and when running Locked methods ignore the HOLD key Transfer Lets you set a method to be transferred to in one of three ways immediately on receipt of a Transfer contact closure input at the end of the current method pass on receipt of a Transfer input automatically on completion of all the method passes Safety Allows you to assign a safety method for any of the following conditions Stop input High Pressure Limit Low Pressure Limit Pump Of
64. olvent Miscibility Solvents should mix with each other in all proportions This is important during elution and during solvent changeover Refer to Figure 26 on page 118 for miscibility of some common HPLC solvents Acetic acid Acetone immiscible Acetonitrile Benzene Miscible Butyl alcohol Carbon tetrachloride Chloroform Cyclohexane Cyclopentane Dichloroethane Dichloromethane Dimethylformamide Dimethylsulfoxide Dioxane Ethylacetate Ethyl alcohol Di ethylether Heptane Hexane Methyl alcohol Methylethyl ketone Iso octane Pentane Isopropyl alcohol Di Propylether Tetrachloroethane Tetrahydrofuran Toluene Trichloroethane Water Xylene o Z e 2 _ je o c EIS 9 9 6 lt lo x Im rs c 5 1915 6 o9 5 0 5 9 Sia 2 5 5 rim s 2 le E5 ESsSZ 8sia s55 S2 lalele pad Lc aak 91012 018 215 65 815 9 8 2 o yo loo 5 2 9 9 cic 5 5 0 gt lt 15 65 5101 00 5 161010515 5 9 9 gt gt 01915 02 lt 512120 S o o Sic ziz f c m m Io 210 65 515 l ozo el x Fe 4 2156 2 9129 255 gt SL L
65. p and HPLC system symptoms with possible causes and suggested corrective actions In most cases you will be able to correct the problem However sometimes the symptom will remain after you have tried the corrective action In these cases please contact your local Agilent office 62 218 Solvent Delivery User Manual Troubleshooting and Diagnostics 6 Using the Pressure Display as Diagnostic Tool The sensitivity of the pressure display is within 68 9 kPa 10 psi The pressure display can be used as a diagnostic tool Following issues cause characteristic pressure fluctuations in HPLC systems parameters valid for HPLC operating at normal pressure Zero to several hundred kPa Bubbles in the solvent 68 9 275 8 kPa Sticking check valve If fluctuations gt 68 9 kPa try to eliminate bubbles in the solvent see Clearing Air Bubbles from the Liquid Head on page 80 If this does not solve the problem a check valve is probably sticking It may be possible to rectify this problem by cleaning the check valve see Cleaning Check Valves on page 92 218 Solvent Delivery User Manual 63 6 Troubleshooting and Diagnostics Troubleshooting Guide Troubleshooting Guide The troubleshooting guide is divided into sections related to symptoms observed in the following units Electronics Liquid flow system Detector signals Electrical Symptoms Pump Dead Table 11 Pump dead Probable cause Solution Power cord
66. properties of each solvent and how to handle it refer to the relevant Material Safety Data Sheets MSDS Be sure to handle each solvent properly Wear proper personal protective clothes e g safety goggles so that a solvent will not come into direct contact with the skin Ventilate the laboratory room adequately to prevent accidental inhalation of harmful solvent vapor Cuts When working with glass or quartz parts take care to prevent breakage 116 Other Precautions Airflow to the cooling fans of the liquid chromatograph must be unobstructed Do not block the ventilation grills on the liquid chromatograph and accessories Consult the manuals supplied with your PC monitor and for their specific ventilation requirements 218 Solvent Delivery User Manual Appendix 10 High Pressure Hazards WARNING High velocity stream of volatile and or toxic liquids If a line ruptures a relief device opens or a valve opens accidentally under pressure potentially hazardous high liquid pressures can be generated by the pump Wear personal protective equipment when you inject samples or perform routine maintenance Never open a solvent line or valve under pressure Stop the pump first and let the pressure drop to zero Always keep the doors and covers closed during operation Read and adhere to all Notes Cautions and Warnings in the manual 218 Solvent Delivery User Manual 117 10 Appendix S
67. r lower p WP 10 32 Fitting standard 2 EA 7727777777277 A Figure 7 Section view of analytical mixer body Solvents from the pumps enter the mixer via the two inlet ports at the base of the mixer body They flow into the lower mixing chamber where they are mixed by the rotation of the lower stir bar The pressure from the pumps forces the mixed mobile phase upward through the spacer into the upper mixing chamber where additional mixing is performed by the opposite rotation of the upper stir bar The fully mixed mobile phase is then forced through a 2 um frit in analytical and narrowbore mixers through the piston and out to the rest of the HPLC system Since the spacer the stir bar cage on preparative mixers assures isolation between the two mixing chambers the mixer acts as a two stage filter for solvent composition noise It is more effective in averaging and reducing solvent composition noise than a single chamber mixer of equivalent volume The passive seal used in the mixer is a hollow molded plastic ring with a circular groove containing an energizing spring The side of the seal containing the spring faces into the mixer chambers When the mixer is unpressurized 218 Solvent Delivery User Manual Introduction 1 the small spring inside the seal maintains contact with the mixer bore and the piston with enough force to seal at low pressures but not excessive force to prevent the seal from sliding
68. ransfer leave this entry blank 4 Press the Method key to leave this sub menu and return to the Method Menu Assign a MODE In NORMAL mode the master pump will operate normally and respond to pressure flow and compositional information from the other pumps in the HPLC system DEMO mode is useful for method development This mode allows you to create and run methods which control other pumps without those pumps being physically present Also a single pump demo method can be run without the pump operating the display will indicate the changing conditions current time flow rate etc but the drive mechanism will not engage 1 Use the LEFT ARROW or RIGHT ARROW key to move to the MODE field 2 Select between NORMAL or DEMO 218 Solvent Delivery User Manual 51 4 Using Sample Methods The following pages contain sample methods and a blank method sheet 52 218 Solvent Delivery User Manual Sample Methods 00 0 eum uonoefui uo oefui uo uonisinboe pasn JORJUOD 1 WO 7 x gi uu 80 syueuiuo Proteins on C8 Figure 12 53 218 Solvent Delivery User Manual Using 4 Sample Methods senum g Q japioaer uni Z 1eduresojne YS 1 WOO x Qr gy gro 5
69. ressure dynamic mixers designed for binary and ternary gradient HPLC and preparative HPLC systems see Figure 7 on page 20 The unique design of the mixer employs a motor driven magnet oriented perpendicular to the mixing chambers As the magnet turns it causes two magnetic stir bars inside the chambers to rotate by radial drive rather than by axial drive as in other dynamic mixers The close proximity of both stir bars to the rotating drive magnet and the fact that the stir bars rotate in opposite directions ensures continuous and thorough mixing A unique piston type closure on the outlet of the mixer allows easy disassembly without tools for cleaning and maintenance On analytical and narrowbore mixers the piston incorporates a 2 um solvent filter to protect system components from contamination The mixer is designed to be plumbed into an HPLC system between the pumps and the injection valve Titanium and PEEK mixers are available for applications where 316 stainless steel may be inappropriate because of corrosion or release of metal ions into solution Titanium or PEEK plumbing components can be used together with PEEK tubing to provide a totally iron free fluid path 218 Solvent Delivery User Manual 19 1 Introduction Piston Drive magnet 20 10 32 Fitting extra long s J NS g 017 re Mixer ca 5 Seal retainer FA Frit Stir bar upper AZ Spacer inte Stir ba
70. s you wish to change only a single parameter or condition 1 Press the RIGHT ARROW key to reach the COPY OF Meth field and then use the UP ARROW or DOWN ARROW to scroll through a list of existing method numbers OR If you already know the method you wish to duplicate enter the number with the number keys Display PASS This is not editable but is a display of the current pass if more than one of the method 1 See ZTIMES step 1 on page 48 Set TIMES 1 Use the LEFT ARROW or RIGHT ARROW key to move to the TIMES field 2 Enter the number of times you wish the method to execute before stopping When the method is running the PASS field will increment for each time the method executes Set the ACCESS level This field lets you set the access level or status of the method 1 Use the LEFT ARROW or RIGHT ARROW key to move to the ACCESS field 2 Choose between UNLOCK and LOCK 218 Solvent Delivery User Manual Using 4 Unlocked methods can be edited and changed even while running Locked methods cannot be edited and the steps are protected from change The Hold key will be ignored when a Locked method is running Assign a TRANSFER Method 1 Use the LEFT ARROW or RIGHT ARROW key to move to the TRANSFER Meth field 2 Press the DOWN ARROW Meth TRANSFER MODE V 0 IMMED DEFER Figure 11 Sub Menu to set transfer method 3 Setthe method to be transferred in the event of a TRANSFER signal to the Agilent 218 or autom
71. strokes recorded by the pump software divided by 1000 HRS Cumulative operational hours recorded by the pump software 78 218 Solvent Delivery User Manual Maintenance and Repair 7 Adjusting the Flow Rate on the Pump When Tools required The flow rate can be adjusted using the compressibility compensation parameters One may achieve this by applying pressure to the pump using a column a long piece of small diameter analytical tubing or a restrictor valve Operational qualification Description Restriction column small diameter tubing or restrictor valve Flow meter volumetric flask or balance Flush the system with water If you want to adjust the flow rate using a different solvent flush the system with that solvent Enter the values for the compressibility compensation for water or whatever solvent you are using to adjust the flow rate Using the SETUP key and adjust the x value to 46 and the L value to 3231 For a different solvent use the compressibility values provided in Table 29 on page 119 Put a restriction into the flow path that provides between 2000 psi 13 8 MPa and 3000 psi 20 7 MPa pressure This can be a column a long piece of small diameter tubing or a restrictor valve Set the flow rate to 1 mL min for the 5 or 10 mL pump heads and whatever flow is normally used for larger pump heads and start the pump Allow the pressure to come to equilibrium Measure the flow rate accuratel
72. sure module flow 50 mL min max pressure rating 4000 psi wetted material PEEK FEP 393553001 Pressure module flow 100 mL min max pressure rating 2000 psi wetted material PEEK FEP 393650501 Pressure module flow 200 mL min max pressure rating 4000 psi wetted material Titanium FEP 104 218 Solvent Delivery User Manual Parts 8 Mixer p n Description 393554601 Mixer volume 0 6 mL material SST wetted materials 316 Stainless Steel Teflon Hastelloy Fluoroloy 12 type Narrowbore 393554701 Mixer volume 0 6 mL material Titanium wetted materials Titanium Teflon Fluorowhite type Narrowbore 393554801 Dual chamber mixer volume 1 2 mL material SST wetted materials 316 Stainless Steel Teflon Hastelloy Fluoroloy 12 type Analytical 393555001 Dual chamber mixer volume 1 2 mL material PEEK wetted materials Titanium Teflon PEEK UHMWPE Fluorowhite type Analytical 393554901 Dual chamber mixer volume 1 2 mL material Titanium wetted materials Titanium Teflon Fluorowhite type Analytical 393555201 Dual chamber mixer volume 10 mL material PEEK wetted materials PEEK Teflon Perfluoro Fluorowhite type Preparative 393555101 Dual chamber mixer volume 10 mL material Titanium wetted materials Titanium Teflon Perfluoro Fluorowhite type Preparative 218 Solvent Delivery User Manual 105 8 Parts Standard Accessory Package p n Description 393550991 Standard
73. te However a leaking piston seal can also result in low flow rate and is more common than check valve failure Inspect for a leaking seal before replacing the check valve Tools required Description Appropriately sized open ended wrench delivered with the pump head Preparations Pump head is already removed CAUTION Damage to check valve cartridges Do not disassemble check valve cartridges Reassembly requires strict cleanroom conditions and great expertise Sub assemblies are not available for check valve cartridges 1 Remove the inlet and outlet check valves they are threaded into the liquid head 2 Remove the check valve cartridge If needed use compressed air to remove the cartridge Do not use a sharp tool that could damage the check valve or the support 94 218 Solvent Delivery User Manual Maintenance and Repair 3 Insert the new check valve cartridge into the support Outlet check valve Inlet check valve Figure 23 Orientation of check valve cartridges The outlet check valve housing is smaller than the inlet check valve housing and threads 7 into the top of the liquid head The cartridge must be inserted in the check valve housing as shown below The check ball must be above the seat in both types of check valve housing so that it will seat by gravity 4 Tighten the check valves into the liquid head by hand then tighten each liquid head a further 1 8th turn with an appropriately s
74. ted actions Air bubbles through flow cell Bad connections Electronic interference Electrical equipment in circuit cycling on and off Degas solvent Pump to waste at a moderately high flow rate to prime pump Check tubing fittings Check all grounding connections on pump and ensure grounded AC power is supplied to all devices in HPLC system Check for loose connections Ensure instruments are not in direct contact with each other or with vibrating parts Isolate equipment which cycles on and off to a different circuit 218 Solvent Delivery User Manual 218 Solvent Delivery User Manual 7 Maintenance and Repair Introduction to Maintenance 72 Warnings and Cautions 73 Maintenance Schedule 75 Service 005 77 Adjusting the Flow Rate on the Pump 79 Clearing Air Bubbles from the Liquid Head 80 Removing Seals Standard Head 81 Removing Seals Washing Head lt 200 mL min 83 Removing seals 200 mL min Head 85 Replacing Piston Seals Heads lt 200 mL min 86 Replacing Piston Seals 200 mL min Head 88 Breaking In a New Seal 200 mL min Head 90 Breaking In a New Seal Heads lt 200 mL min 91 Cleaning Check Valves 92 Replacing Check Valves 94 Checking and Replacing the Mixer Outlet Filter Frit Analytical and Narrowbore Mixers Only 96 Replacing the Mixer Seal 97 Checking and or Changing Power Fuses F1 98 This chapter describes the maintenance of the instrument te Agilent Technologies
75. the liquid head although this sign may not be present if the solvent leak evaporates quickly Seal wear is accelerated under adverse conditions such as pumping at high flow rates or pressures using aggressive or aqueous solutions or dirty or contaminated mobile phase Moderate operation low flow low pressure organic solutions and fresh clean HPLC grade mobile phases will result in longer seal life However every seal will eventually need replacing Software in the Agilent 218 Pump allows you to both check the seal wear and to log when the seals are changed Your pump uses either a standard liquid head or a washing liquid head 76 218 Solvent Delivery User Manual Maintenance and Repair 7 Service Logs Agilent 218 software automatically tracks seal wear check valve use and pump drive wear The software also allows the user to enter and record seal and check valve changes Use the Service Logs 1 Press the SETUP key to open the SETUP SERVICE log display 2 Press the RIGHT ARROW to reach Service Log and then press the DOWN ARROW to see the following display PISTON CHECK SEAL VALVE 3 Select the desired menu by pressing the RIGHT or LEFT ARROW key Table 24 Piston Seal Log Item Function SL 06 Seal Log Displays the sequential number of last seal change DATE Date of last seal change service This value is entered numerically by the user USE Use units since last seal change proportional to number of s
76. tling the mixer or disconnecting the outlet tubing 1 2 Turn off the Agilent 218 Purification Solution Unscrew the mixer mounting panel from the Agilent 218 Purification Solution and disconnect the power connection Unthread the mixer cap by turning it counterclockwise The outlet tubing fitting will still be connected 4 Loosen and remove the seal retainer includes frit Thread the replacement seal retainer and frit onto the piston Finger tighten the seal retainer Press the piston in the mixer body bore Replace the mixer cap 218 Solvent Delivery User Manual Maintenance and Repair 7 Replacing the Mixer Seal When If leaks are seen at the top of the mixer and the correct outlet fitting is swaged properly the seal may need to be replaced Replace the seal The seal can be replaced without fully dismantling the mixer or disconnecting the outlet tubing 1 Turn off the Agilent 218 Purification Solution 2 Unscrew the mixer mounting panel from the Agilent 218 Purification Solution and disconnect the power connection 3 Unthread the mixer cap by turning it counterclockwise The outlet tubing fitting will still be connected 4 Analytical and narrowbore mixers Loosen and remove the seal retainer and frit Remove the seal by pulling it away from the piston Place a replacement seal on the piston so the spring inside the seal is visible If the seal is put in upside down the seal will leak Push the seal over
77. to demonstrate compliance 218 Solvent Delivery User Manual 123 10 Appendix Electromagnetic Compatibility EN55011 CISPR11 124 Group 1 ISM equipment group 1 contains all ISM equipment in which there is intentionally generated and or used conductively coupled radio frequency energy which is necessary for the internal functioning of the equipment itself Class A equipment is equipment suitable for use in all establishments other than domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purposes This device complies with the requirements of CISPR11 Group 1 Class A as radiation professional equipment Therefore there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances Operation is subject to the following two conditions This device may not cause harmful interference This device must accept any interference received including interference that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures Relocate the radio or antenna Move the device away from the radio or television Plugthe device into a different electrical outlet so that the device and the radio or tel
78. trokes and pump pressure LIMIT Use units limit set by user depending on anticipated amount of use CHGD Changed Press the DOWN ARROW then the ENTER key to enter YES when seal change is performed Table25 Check Valve Log Item Function CK LOG Sequential number of the last check valve service check valve cartridge replacement DATE Date of last check valve service This value is entered numerically by the user 218 Solvent Delivery User Manual 71 7 Maintenance and Repair Table 25 Check Valve Log Item Function USE Use units since last check valve service LIMIT Use units set by user depending on anticipated amount of use IO Inlet Outlet Press the UP or DOWN ARROW keys to scroll between the choices to indicate the check valve The check valve is referenced in the next menu item CHGD Scrollable choices are 10 Serviced both check valves YY 10 Serviced the outlet check valve but NY not the inlet check valve 10 Serviced the inlet check valve but YN not the outlet check valve CHGD Changed Press the DOWN ARROW then the ENTER key to enter YES when check Serviced valve cartridge replacement the only check valve user service item is performed Table 26 Pump drive Item Function WEAR FACTOR This indicates wear in the drive Both the pump pressure and the flow rate are recorded by the Agilent 218 software and factored into this indicator KCYLS This is the number of piston
79. window also shows system pressure The left and right arrows at the sides indicate that you can access more information with the left right arrow keys Method status and name to the left Current values for composition for B C D to the right Also 1 0 parameters You can reach more information to the right in two ways Pressthe right arrow to scroll rightward through the entire line Press the 1 0 button below The entire line of information is divided between the FLOW and 1 0 buttons for convenience time is always displayed Pressing the FLOW key when in another display returns the display to the part of the Flow field which was last viewed Pressing the FLOW key a second time returns to the default position with the cursor on the flow rate value 218 Solvent Delivery User Manual 23 1 Introduction Table2 Function keys Key Function 1 0 This key opens the second part of the display Note that you can scroll leftward into the Flow section if you wish The 1 0 section contains the following items from left to right Analog Analog Input This is the current voltage read on the Analog input channel for this pump only Range from 0 5 V to 2 5 V ishtm Input Contact Status Contacts are Inject Stop Hold Transfer and Mark 1 indicates a closed contact 0 indicates an open contact Meth Method status and name repeat information from Flow display above nm This control is used to set wavelength on a su
80. y The Main Method display is visible New Meth COPY OF Meth PASS TIMES ACCESS TRANSFER SAFETY MODE 0 0 1 UNLOCK Meth Meth NORMAL LOCK DEMO Meth TRANSFER MODE v 0 IMMED DEFER Meth STOP HIGH P LOW P OFF LINE V 0 Figure 9 Main Method display You will not see all this information at once but can access the off screen portion of the display with the RIGHT ARROW key The DOWN ARROW is used to select menu choices such as ACCESS its choices are UNLOCK and LOCK The TRANSFER and SAFETY method sub menus are also shown These sub menus are used to specify which method is to be used when there is a Transfer or Stop signal received by the pump and are also opened by pressing the DOWN ARROW key The New status and COPY OF Meth appear only when you press the New key Once a method is created these items do not appear in the display Preparing Creating a Method 1 Press the METHOD Key The default method display is open 46 218 Solvent Delivery User Manual Using 4 Meth S MM Figure 10 Default method display This display shows that the Agilent 218 Purification Solution is in Manual Method status the default state which allows you to operate the pump manually by entering flow values and pressing the RUN key Cursors around MM are used to select between EXISTING methods The first time you open this display these cursors will be inoperable as there are no methods pro
81. y using either a calibrated flow meter a calibrated volumetric flask or by weighing solvent on a calibrated balance If the measured flow rate is higher than the set flow rate lower the value of the x parameter To raise the actual flow rate increase the value of the x parameter For water an increase or decrease of about 20 will lower the flow rate about 0 5 to 1 For other solvents the x parameter adjustment value will vary Continue to adjust the x parameter until the actual flow rate falls within the pump specification of 1 of the set flow rate 218 Solvent Delivery User Manual 79 7 Maintenance and Repair Clearing Air Bubbles from the Liquid Head If you notice pressure fluctuations from zero to several hundred kPa when the HPLC system is operating at normal pressure there is probably a bubble in the liquid head WARNING Chemical burns to eyes skin and or respiratory tract The mobile phases in the system may be hazardous gt Observe all standard laboratory safety precautions Always wear personal protective equipment 1 Operate the pump at moderate flow 10 of maximum with the system pressurized 2 Carefully loosen the outlet check valve fitting just enough to make it leak also known as crack the fitting Figure 18 Outlet check valve fitting You should see solvent sputtering at the fitting as the bubble leaks past the fitting 3 When the bubble is clear the sputtering will stop and the

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