Operator`s Manual The Bullet Blender™
Contents
1. polypropylene conical bottom Falcon or Krackelerbrand 50 mL tubes loaded with samples in the holes in the sample plate and close the cover Fifty mL tubes from other Cover Plexiglas Enclosure manufacturers may not work properly due to differences in length or toughness The snap caps on the microcentrifuge tubes must be on securely The threads on the 50 mL tubes must be dry and the caps must be screwed on very tightly The tubes do NOT need to be placed symmetrically The tissue placed n each tube should usually be cut into several smaller pieces Set the duration in minutes and the speed to the desired value Then push the start button As each minute passes another LED will light up On the standard model when the set time has passed the green light below the word DONE will On the BLUE models the air cooling will continue for half blink for a few seconds NEXT gt ADVANCE 3 OPERATOR S MANUAL FOR THE BULLET BLENDER a m nute Do not operate with the cover open There will be excess noise and your samples will not be properly processed and the tubes might be knocked entirely out of the instrument and could cause injury Close the cover gently with the lock knob aligned so that the pin in the cover fits through its slot Then rotate the knob to lock the cover closed If you turn the minutes knob to 0 the instrument will stop Example NEXT gt gt gt minutes Con2. DISCLAIMER Next Advance Inc makes no representations or warranties expressed statutory or implied regarding the fitness or merchantability of this product for any particular purpose In no event shall Next Advance be liable for incidental or consequential damages Next Advance Inc is not liable for any damages including but not limited to lost profits lost savings or other incidental or consequential damages arising from ownership or use of this product or for any delay in the performance of its obligations under the warranty due to causes beyond its control Next Advance Inc also reserves the right to make any improvements or modifications to the product described in this manual at any NEXT gt ADVANCE 6 OPERATOR S MANUAL FOR THE BULLET BLENDER time without notice of these changes Next Advance Inc products are not designed intended or authorized for use n applications or as system components intended to support or sustain human life as a clinical medical device for humans or for any application in which the failure of the product could create a situation where personal injury or death may occur All brand and product names used in this manual are the trademarks of their respective OWNETS CONTACT INFO Next Advance Inc Averill Park NY USA Telephone 518 674 3510 www nextadvance com Email info nextadvance com NEXT gt ADVANCE BBmanual_CE_v090622 NEXT gt gt gt HDVANCECE
3. Operator s Manual The Bullet Blender Models Bullet Blender Bullet Blender Blue and Bullet Blender Blue 50 BBmanual_CE_v090622 NEXT gt gt gt HDVANCEE OPERATOR S MANUAL FOR THE BULLET BLENDER Congratulations Congratulations on your purchase of a Bullet Blender by Next Advance Inc for mixing lysing disrupting and homogenizing your samples It comes with beads of several sizes and materials and a spatula Please read this operator s manual which explains proper operation of the instrument This manual is posted on our website www nextadvance com Click on SUPPORT in the left window and then on the appropriate link to the manual We re confident that your Bullet Blender will work out well for you and we wish you success with your work Warranty Contents Warnings and Cautions ccccccccnnnccncnnnnnnnnnnnno Disclaimer Contact Info NEXT gt ADVANCE OPERATOR S MANUAL FOR THE BULLET BLENDER Parts of the Bullet Blender Sample Tubes Sample Plate Control Box SETUP Place the Bullet BlenderiM on a stable level lab bench Plug the power supply into the back of the Bullet Blender and then plug it into a wall outlet It is now set up OPERATION To use your Bullet Blender lift open the cover place up to 24 of your 1 5 mL to 2 0 mL polypropylene high quality e g Eppendorf Safe Lock or microcentrifuge tubes or up to 9 Axygen
4. be is important and without an airs space you will not get effective homogenization You must leave at least 500 microliters of airspace in a microcentrifuge tube to get effective homogenization For similar reasons do not fill the 50 mL tubes with more than a total of 15 mL of buffer tissue and beads Protocols for many types of samples are posted on the wwww nextadvance com website and click on Support NEXT gt ADVANCE 4 OPERATOR S MANUAL FOR THE BULLET BLENDER Notes At speed settings of 9 and 10 there may be some flaking of the microcentrifuge tubes This s normal and intentional The higher speed enables homogenization of tougher tissue Use high quality polypropylene tubes such as Axygen microtubes and Falcon or Krackelerbrand 50 mL tubes When you first plug in the standard Bullet Blender the green light may blink for a In Bullet Blender M Blue models the fan will run for few seconds This s normal about a minute and the blue Air Cooling indicator will light up The cover may rattle while the Bullet Blender is operating This is normal and inherent in the design in which all tubes are allowed to oscillate independently CLEANING The light blue cylindrical Plexiglas enclosure is sensitive to many alcohols and solvents Clean the outside of the unit only with mild soap water and a soft cloth If liquid enters the instrument remove the sample tube plate with a hex wrenc
5. h 4 screws wipe out the spill using standard laboratory safety precautions and replace the sample tube plate TROUBLESHOOTING In addition to the tips given below a thorough list of troubleshooting tips is at http www nextadvance com FAQs FAQs Bullet_Blender htm If nothing happens the power supply plug might not be in a live wall outlet or the power supply connector plug might not be fully in the jack on the back of the Bullet Blender M If the unit stops working turn the system off for 15 minutes to allow the electronics to reset If the Bullet Blender does not turn on after this period contact customer service If the caps on the microcentrifuge tubes pop open or the caps on 50 mL tubes loosen up make sure that the interface regions or screw threads between the lids and the caps is dry when you close the caps or screw them on so that there is enough friction for the caps to remain tight SUPPORT A thorough list of FAQs and protocols are available on our website http www nextadvance com Click on support and then on the appropriate link If you cannot find an answer there please contact customer service by email at techsupport nextadvance com or by telephone 1 518 674 3510 NEXT gt ADVANCE 5 OPERATOR S MANUAL FOR THE BULLET BLENDER SPECIFICATIONS S ze 8 n 20 cm diameter by 6 0 n 15 cm or 9 in 23 cm high depending upon model Weight 2 6 to 3 4 kg 5 7 to 7 5 lbs dependi
6. ng upon model Power Requirement 24 VDC 0 75 Amp Capacity 24 of 1 5 2 0 mL poly propylene high quality e g Eppendorf sample tubes or 9 of 50 mL polypropylene Krackelerbrand or Falcon sample tubes depending upon model Relative Humidity 3 90 non condensing Operating Temperature 4 50 C Storage Temperature 40 to 50 C Meets CE requirements WARRANTY The Bullet Blender comes with a 30 day money back guarantee less shipping charges and a two year warranty There is a 3 year warranty on the motor Next Advance will replace free of charge any part which is defective due to workmanship or materials For further http www nextadvance com legal_terms ht information go to m Warranty is void if Product has defect or damage due to product acc dent alteration connection to an improper electrical supply fire flood lightning or other conditions beyond the control of Next Advance Product is improperly installed or used Operator s Responsibility Provide proof of purchase and provide normal care and maintenance WARNINGS AND CAUTIONS Read the user s manual No user serviceable parts are inside For indoor use only Pollution Degree 2 per EN 61010 1 Sound Pressure Level up to 90dBA Use hearing protective devices that reduce exposure to below 85 dBA during prolonged exposure Do not immerse in liquid Before touching the Bullet Blender discharge static by touching ground
7. trol panel set for a 4 minute duration at a speed of 8 The Bullet Blender has been running for 2 minutes The figure above shows the Bullet Blender set to run for 4 minutes at a speed of 8 after running for 2 minutes To operate press the START button After the first minute the red light by the number 1 will light up After the second minute the second red light by the number 2 will light up And so on After 4 minutes the Bullet Blender M will stop In the standard version the green word DONE will light up for half a minute In the Blue versions the blue words Air Cooling word will light up during operation and for about one half minute as the fan continues to operate The model that accommodates 50 mL tubes has time settings up to 15 minutes in 3 minute increments PROTOCOLS AND SAMPLE SETTINGS General Guideline 1 volume mass of tissue 1 volume or 2 mass of grinding media 2 volumes of buffer As the tissue amount becomes smaller that ratio will differ due to the limitations of handling of the small volumes Cutting the tissue into smaller pieces will generally yield better results With microtubes we recommend less than 300mg that is 0 3g of tissue per microtube in the Bullet Blender When the tissue is overloaded there is not enough airspace in the tube to allow for efficient mixing of the sample with the grinding agent The movement of the liquid around the walls of the tu
Download Pdf Manuals
Related Search