Wistar Fetal Rat Cortex Neuros
Contents
1. 5 Use a pipette to transfer 1mL Neurons Growth Medium to the vial drop by drop in side a biosafety cabinet mix by gently pipetting Subsequently transfer this 2mL of cell suspension into the conical tube containing Neurons Growth Medium drop by drop Be careful not to introduce any bubbles during the transfer process 6 Rinse the vial with 1 mL of medium to reduce cell loss Subsequently transfer this 1mL of cell suspension to the conical tube 7 Gently mix the cell suspension by slowly pipetting up and down Be careful not to introduce any bubbles 8 Take a small sample of the cells from the cells suspension Determine the viable cell density using vital staining assay with trypan blue 9 Plate around 1x 10 cm viable cells in poly L lysine Laminin coated plate Gently rock the culture plate to evenly distribute the cells A Note Do not centrifuge the cells as they are extremely fragile upon recovery 10 Incubate the cells at 37 C in a 5 CO humidified incubator 11 Six hours later change half of the medium with fresh OriCell Neuron Growth Medium pre warmed to 37 C Change half of the medium every three days or change the entire medium every three days if there is any cell death TM Fig 1 OriCell Wistar Fetal Rat Cortex Neurons are established IMPI0051A2 WCCFN 00001 Page 5 of 6 y cyagen APPENDIX Related products Product Catalog Number OriCell Wistar Fatal Rat Cortex Neurons WCCFN 00001 OriC2. Aspirate after each rinse 6 Use sterile 1X PBS dilute the Laminin stock Solution 1mg mL to a final concentration of 15ug mL 7 Add enough of Laminin Solution into the culture vessel to completely cover its base Incubate overnight at 4 C coated vessels can be stored in the Laminin Solution at 4 C for up to one week 8 Just before use aspirate the Laminin Solution in the coated vessels and wash the wells once with 1X PBS Aspirate after rinse THAWING AND ESTABLISHING OriCell WISTAR FATAL RAT CORTEX NEURONS Materials Required e OriCell Wistar Fatal Rat Cortex Neurons Cat No WCCFN 00001 e OriCell Neuron Growth Medium Cat No GXXNR 90011 Thawing and Establishing Wistar Fatal Rat Cortex Neurons 1 Pre warm the OriCell Wistar Fatal Rat cortex Neuron Growth Medium to 37 C 2 Remove the cryovial of OriCell nitrogen Wistar Fatal Rat Cortex Neurons from liquid 3 Quickly thaw the vial in a 37 C water bath until the last ice crystal disappears For IMPI0051A2 WCCFN 00001 Page 4 of 6 y cyagen optimal results be sure to finish the thawing procedure within 3 minutes Be careful not to submerge the entire vial Maximum cell viability is dependent on the rapid and complete thawing of frozen cells Note Thawing the cells for longer than 3 minutes results in less than optimal results 4 As soon as the cells are completely thawed disinfect the outside of the vial with 70 v v ethanol
3. AS C y a I 6 il We help you discover life oricel Wistar Fetal Rat Cortex Neurons Cat No WCCFN 00001 y cyagen Table of Contents Contents and StOrgg siii iaa 3 PFOGUCE Introduct N siinnniana a a ai 3 PFOGUCE ADDICACIONS rinitis 3 General Handling Principles sicario A 4 Preparing Growth Medium and Culture Vessels Poly L lysine Laminin Coating of Tissue Culture Plate sssssssssnssnnnnnnnnnnunnnnnnnnnnnnnnnnnn 4 Culturing OriCell WISTAR Fatal Rat Cortex Neurons Thawing and Establishing OriCell WISTAR Fatal Rat Cortex NeuroNS mmmmmmm 4 Velo cp a cere errr rt rer rer tr te er ttre rer cree errr ret tat rrr rrr 6 Related Products imss 6 ROTerences min as O TECHNICAL auna Y Ocvagen CONTENTS AND STORAGE product Name Wisrar Fatal Rat Cortex Neurons Catalog No WCCFN 00001 Amount per Vial 1x10 Cells Cryopreserved At Primary Passage Storage Condition Liquid Nitrogen CAUTION Please handle this product as a potentially biohazardous material This A product contains Dimethyl Sulfoxide DMSO a hazardous material in the freezing medium PRODUCT INTRODUCTION Neurons also known as nerve cells connect to each other to form networks They are the main components of the nervous system and are potent tools in studying function of the neural system Cyagen OriCell Wistar Fatal Rat Cortex Neurons are derived from the cortex of qualified Wistar rat embryos 18 5 dpc and cryopreserved a
4. ell Wistar Fetal Rat Hippocampus Neurons WHCFN 00001 OriCell Neuron Growth Medium GXXNR 90011 References Greory J Brewer and John R Torricelli 2007 Isolation and culture of adult neurons and neurospheres Nature 2 1490 1498 CHENGSONG XIE William R Markesbery and Mark A Lovell 2000 Survival of Hippocampal and Cortical Neurons in a Mixture of MEM and B27 Supplemented Neurobasal Medium Free Radical Biology amp Medicine 28 665 672 Cyagen Biosciences reserves all rights on the technical documents of its OriCell cell culture products No part of this document may be reproduced or adapted for other purposes without written permission from Cyagen Biosciences IMPI0051A2 WCCFN 00001 Page 6 of 6
5. s primary cells These cells express specific clusters of different proteins for neurons They have been tested negative for bacteria fungi and mycoplasma pu Viability of Cyagen OriCell Wistar Fatal Rat Cortex Neurons e Recovery Viability 50 Ts Purity of Cyagen OriCell Wistar Fatal Rat Cortex Neurons e MAP2 positive cells gt 70 e tubulin III positive cells gt 70 e GFAP positive cells lt 10 This product is intended for laboratory research use only It is not intended for diagnostic therapeutic clinical household or any other applications IMPI0051A2 WCCFN 00001 Page 3 of 6 y cyagen PRODUCT APPLICATIONS OriCell Wistar Fatal Rat Cortex Neurons can be used in neurobiology studies and drug discovery in areas such as Parkinson s Disease and Alzheimer s Disease GENERAL HANDLING PRINCIPLES Aseptic handling of the product is necessary throughout POLY L LYSIN LAMININ COATING OF TISSUE CULTURE PLATE 1 The day before plating cells for differentiation prior to prepare the coated plates with PLL laminin Dilute Poly L lysine stock Solution 1mg mL with water to yield 15ug mL solution 3 Add enough of Poly L lysine Solution into the culture vessel to completely cover the base 4 Swirl until Poly L lysine Solution coats the entire base of vessel Sit for at least 30 minutes at room temperature 5 Aspirate off all of the Poly L lysine Solution and rinse the vessel once with sterile water
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