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Recorder Rec 111 Recorder Rec 112 Recorder Rec 111 Recorder

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1. WARNING Do not block the rear panel of the system The mains power switch must always be easy to access Ensure that the protective mains earth is functioning correctly before you connect the equipment to the mains Connect the instrument to a protective earth conductor via the three core mains cable before making any other connection Insert the mains plug only into a socket outlet provided with a protective earth contact Do not use an extension cord without a protective conductor WARNING Any interruption of the protective conductor inside or outside the instrument or disconnection of the protective earth terminal is likely to make the instrument dangerous Intentional interruption is prohibited Make sure that only fuses of the required type and current rating 250 mA for 100 120 V operation and 125 mA for 220 240 operation are used for replacement The use of repaired fuses and or short circuiting of fuse holders is prohibited Fuses should only be replaced by qualified persons who are aware of the hazards involved Note Adapting the mains plug to local conditions should only be done by a qualified person WARNING Disconnect the instrument from all sources when replacing a fuse or when adapting the instrument to a different voltage 3 3 Electrical connections The instrument is supplied with mains cable and fuses for both 100 120 V and 220 240 V operation 1 Ensure that the mains p
2. 6 2 Recycling This symobl indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the 6 3 Chart drive Symptom decommissioning of equipment Cause Remedy No chart drive 1 Key rec off on is in off position Key ext is pressed and no generator is used Chart or chart roll blocks drive mechanism Set key to on position Release ext key Check 4 External generator defective or Check generator output amplitude too low output 5 External generator connected by Check using wrong cable or connection 6 The remote chart ON OFF signal Check is in the OFF level 6 4 Chart speed Symptom Cause Remedy Incorrect chart speed 1 Incorret setting of chart Correct setting speed unit 2 Incorrect pulse rate from Check generator external frequency generator 3 Extreme pulse rate Check and see section Control in external mode mode by external control pulses Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 25 Trouble shooting 6 5 Pen system Symptom Cause Remedy Pen does not touch chart 1 Pen up down key is in wrong Check position 2 Pen holder bent or pen wrongly Adjust gently assembled in holder 3 rec off on position Check 4 Ext pen lift signal is active
3. position the chart drive must be fed with pulses from an external source see Section 3 6 2 4 4 Paper transport The keys for this function are marked with two arrows With these keys the paper can be accurately positioned underneath the pens Each time one of these keys is pressed for less than one second the paper moves a distance of 0 05 mm forwards or backwards If the key is depressed for longer than one second the paper will move at a speed of 2 mm s The paper will continue to move at this speed for the next five seconds corresponding to a distance of 10 mm After depressing the key for six seconds the paper will start moving at a speed of 20 mm s These paper feed keys facilitate quick and accurate positioning of the paper under the pens The paper can only be transported in this manner when the rec off on key is in the off position u 2 4 5 Home and set Pressing the home key will return the pens to where the origin is located This is either the position of the pens during powering up or the position of the pens at the moment key set was last pressed This key is used to define a new origin at any position These keys can only be operated when the rec off on key is in the off position 2 4 6 Goto next grid mark This function is activated by pressing grid Each time this key is pressed the paper advances 10 mm which is the distance between the grid marks printed on the recorder paper When the ori
4. 3 4 Ln Switch on the mains power Set the zero key s to the down position Select a suitable low chart speed 0 5 2 mm min Set the pen s down and make a coarse zero adjustment to the right hand zero on the chart Set rec off on to the on position and make a final zero adjustment 6 Set rec off on to the off position Use the forward feed key to align the short nib 10 pen with a grid line and press the set key i e define your start position Set the zero euls in the up position Select the chart speed with the chart speed switch and mm s min key If an inbetween voltage range is required Select the nearest higher voltage range Set the cal var key to the var position and calibrate against a known standard or the peak voltage of your recording signal The recorder is ready to use by setting rec off on key to on 4 2 Operation in external mode by external pulses 1 2 3 4 Ln Switch on the mains power Set the zero key s to the down position Select a suitable low chart speed 0 5 2 mm min Set the pen s down and make a coarse zero adjustment to the right hand zero on the chart Set rec off on key to the on position and make a final zero adjustment 6 Set rec off on to the off position Use the forward feed key to align the short nib pen with a grid line and press the set key i e define your start position
5. Check 5 Friction with slide Clean slide bar and pen carriage DO NOT USE LUBRICANT 6 Pen lift mechanism obstrycted Consult GE Healthcare distorted representative Symptom Cause Remedy Pen is not adjustable over full scale 0 100 96 with zero adjustment wheel 1 Excessive input voltage Reduce input voltage or correct the range setting 2 Servo potentiometer pulley Consult GE Healthcare loose from shaft representative Dead zone 1 Slide bar dirty Clean with cloth DO NOT USE LUBRICANT 2 Teeth gearwheel damaged Consult GE Healthcare representative Pen moves irregularly 1 Supplied signal irregular Check 2 Slide bar dirty Clean with cloth DO NOT USE LUBRICANT 3 Teeth of gearwheel damaged Consult GE Healthcare representative Pen is not adjustable with zero knob motor running 1 Drive cord broken Consult GE Healthcare representative 2 Drive spring drive cord worn Consult GE Healtcare representative 26 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD Trouble shooting Symptom Cause Remedy Pen deflection does not correspond to chosen sensitivity 1 Input voltage not correct Check 2 Selector key var pressed Check Pen is creeping Pen overshoots Pen vibrates Home and grid function fails 3 Calibration attenuator incorrect 1 Slide bar dirty 2 Damping not correct 1 Damping not correct 1 Excessive AC voltage on input terminals 1 Mains voltage interrupt 2 Excessive level of electr
6. 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 Austria Tel 01 57606 1619 Fax 01 57606 1627 Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 800 463 5800 Fax 800 567 1008 Central East amp South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 e Denmark Tel 45 16 2400 Fax 45 16 2424 Finland amp Baltics Tel 358 0 9 512 39 40 Fax 358 0 9 512 39 439 France Tel 01 69 35 67 00 Fax 01 69 41 96 77 Germany Tel 0761 4903 490 Fax 0761 4903 405 Italy Tel 02 27322 1 Fax 02 27302 212 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 e Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 9600 687 Fax 30 210 9600 693 e Netherlands Tel 0165 580 410 Fax 0165 580 401 lt Norway Tel 815 65 555 Fax 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 e South East Asia Tel 60 3 8024 2080 Fax 60 3 8024 2090 Spain Tel 93 594 49 50 Fax 93 594 49 55 e Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 12 Fax 0848 8028 13 UK Tel 0800 616928 Fax 0800 616927 USA Tel 800 526 3593 Fax 877 295 8102 imagination at work E Elanders Tofters 2006 User M
7. 20 Edition AD 11 General Description 2 3 Left hand side view 25 24 Fig 3 Model REC 112 No Item Description 24 Mains power switch Switches the recorder ON OFF 25 Fuse holder 100 120 V fuses 250 mA 220 240 V fuses 125 mA 2 4 Chart drive description 2 4 1 Record off on The function of this key is to start and stop paper transport When rec off on key is in the off position it interrupts both the internal chart speed pulses and the external pulses This key must be set in the off position to operate any of the following functions feed arrows home grid and set When rec off on is in the on position the above keus will not operate 2 4 2 Chart speed The velocity at which the paper moves is controlled by a combination of two functions One is chart speed of which there are eight possible positions between 0 1 and 20 units The other is the applied unit which is either mm min or mm s Together these give a lowest speed of 0 1 mm min at which the paper roll has an expected lifetime of 4 100 hours and a highest speed of 20 mm s at which the paper roll lasts approx 20 minutes 12 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD General Description 2 4 3 Internal external The position of this key determines the source of the pulses driving the paper When the key is in the int position an internal counter is in operation When in the ext
8. FSD full scale deflection 2 position key selects between calibrated and user adjustable span Potentiometer to set the range from 40 to 100 FSD by means of a screwdriver 2 position key switches one channel amplifier input to zero used for zero adjustment of the pen The recorder zero may be checked at anytime during operation without changing any other settings The zero is as default set to the right hand side of the chart paper Note The recorder response will not be activated unless the zero key is in the up position Potentiometer with rotating knob used to position the pen on the paper 2 position key used to activate the electrical pen lift pen 1 and pen 2 are independent Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD Geneal Description No Item Description Chart drive left hand side of each channel 7 rec off on 2 position key selects record off mode keyboard control of chart drive forward reverse home zero set and go to major grid mark record on mode internal chart speed settings or external pulse input and remote control functions 8 chart speed 8 position rotary switch for fixed chart speed settings 0 1 0 2 0 5 1 2 5 10 and 20 9 mm s min 2 position key changes selected chart speed unit from mm s to mm min 10 int ext 2 position key changes between keyboard selected chart speed and external chart pulse input 11 feed v Transports the paper for
9. GE Healthcare Recorder Rec 111 Recorder Rec 112 User Manual Important user information All users must read this entire manual to fully understand the safe use of Recorder REC 111 and Recorder REC112 WARNING The WARNING sign highlights instructions that must be A followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected se
10. Set the zero key s to the up position Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 21 Operation 8 Select the appropriate chart speed by calculating Input frequency Hz x chart speed setting x 0 05 mm sec 20 x applied unit mm s min key in mm s applied unit 1 in min applied unit 60 Note Max input frequency is 8 kHz with a pulse duration of min 1 us chart speed setting must not give a chart feed in excess of 20 mm s 9 If an inbetween voltage range is required see point 10 in previous section 10 Set the int ext key to the ext position 11 The recorder is now ready to use by setting rec off on key to on position Note The external Chart ON OFF signal will control the paper feed on off in both panel mode and in external mode when key rec off on is in on position Electrical low signal gnd is Chart OFF as default by internal connection 4 5 Home and grid function 1 Finish your recording by setting the rec off on key to off position 2 To feed the chart to your start position just press the home key Note Make sure that the chart paper moves freely on the rear side of the recorder to avoid disturbing the home function 3 To feed the chart paper to the next grid line just press the grid key Note The functions above are valid provided no mains voltage interruption power off or reset has occurred since the start of your
11. This function allows relocation of the zero point anywhere between the left and right edge of the paper After the zero location has been chosen the recorder must be put back into operation Press the zero once again to release the key Note On delivery the recorder zero position is factory set to right hand zero 2 4 11 Pen up down This key controls vertical movement of the pen If there has been no chart drive pulse for a period of 30 seconds the penis will be automatically lifted off the paper This prevents ink stains on the paper For this function to operate the rec off on key must be in the off position and pen up down key in the down position It also operates when the paper transport is controlled externally and chart drive pulses have stopped Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 3 Installation Installation gt gt 3 1 Unpacking CAUTION The system should be installed on a stable laboratory bench providing a suitable working area Carefully unpack Recorder REC 111 or Recorder REC 112 Check the contents against the packing list supplied Inspect for any damage that may have occurred during transit Report any damage immediately to the local GE Healthcare representative concerned Save the packing material if future transport can be foreseen 3 2 Safety instructions for power connection WARNING Only use mains cables delivered or approved by GE Healthcare
12. and grid fUNCHION emmener 22 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 5 Contents 5 Maintenance S G lt 212 Tare de ETE 23 6 Trouble shooting Bl cus Tc 25 COME e e a D 25 Bo E lt lt Te m E NENNEN PME NI e 25 64 e nisl eee eei eti tot i tie D uL Meu 25 6 5 e EE E Ea nan mare ee ee 26 7 Technical Specifications 8 Accessories and Spare Parts 9 Applications OEM MOM C SO P cR EORR 33 6 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD Introduction 1 Introduction GE Healthcare model REC 111 Code No 18 1132 32 single channel recorder and model REC 112 Code No 18 1132 33 dual channel recorder are compact recorders designed to meet the challenging demands of today s technology These instruments are high quality flat bed recorders from GE Healthcare which use a null balancing potentiometric system They can accommodate a range of input voltages offer a selection of chart speeds and allow many functions to be remotely controlled The recorders have sophisticated features such as electric pen lift grid and home functions forward and reversed paper feed Paper is transported at a constant velocity selected from the 14 different chart speeds in the range from 0 1 mm min to 20 mm s and can be synchronized with the pump to enable chromatogram comparision The positions of the two independent pens REC 112 represent the levels of the two corresponding inputs fro
13. anual 18 1132 20 AD 01 2006
14. d be carried by properly shielded leads to avoid the AC hum and noise Excessive hum and noise at the input may cause the pen to oscillate with high frequency Note When connecting UV Monitors UV 1 and UV M II Conductivity Monitor or pH Monitor from GE Healthcare connect the pin connectors supplied with the recorder to the monitor signal cable s Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 17 Installation 18 3 7 External control The recorders REC 111 and REC 112 can be remotely controlled in a GE Healthcare chromatography system as well as by instruments from other suppliers There is a 15 pin D sub connector on the rear of the recorder which enables remote control of the recorder The following functions are available from the 15 pin D sub connector Signal logic pin numbers Function 15 pin D sub Pin No x 0 Go to grid mark 4 x 0 Event mark channel 1 6 0 Event mark channel 2 7 x Pen lift channel 1 1 x Pen lift channel 2 2 x Chart drive pulse input 14 x 0 Home 8 x Chart ON OFF 12 x 0 Start of plot major grid mark 3 Signal GND 10 15 E only available in rec on mode default active O 1 selected internally by strap O active 0 or contact closure to ground only available when panel keys pen are in pen down position Note In order to supress unwanted interference from external origin all inputs except ECP External Chart drive Pulses and servomarker are fil
15. eed to have such a high frequency Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD Installation There is no maximum duration for the ECP but it is recommended to use pulses of at least 1 usec As soon as the positive edge is received the chart drive will make one step This is only true when the dividend equals 1 When the dividend has a value of N the step will not be made until the positive Nth edge has appeared 3 8 Event mark The recorder models REC 111 and REC 112 are equipped with event mark built into each channel Event mark is performed as a displacement of the servo pen approx 2 10 scale divisions to the right As soon as the function is de activated the pen moves back to the position that represents the channel input value Note This is true if the zero position is on the right hand side of the paper If it is on the left hand side of the paper then the pen will move to the left when the event mark function is activated Event mark is performed by pin 15 and pin 6 on the 15 pin D sub connector for channel 1 For channel 2 event mark is performed by between pin 7 and pin 10 on the 15 pin D sub connector When the recorder is used together with any other GE Healthcare instruments please refer to the User Manual for the instrument concerned or contact your local GE Healthcare representative for further information 3 9 Chart drive GE Healthcare pumps i e HiLoad Pump P 50 and Pump P 1 HPLC pu
16. gin has been set precisely over a grid mark pressing grid will always advance the pen to the next grid mark Paper is transported at a velocity of 20 mm s This function only operates when rec off on key is in off position 2 4 7 Servo system The REC 111 112 is equipped with two fully independent servo systems Each servo system has an operator panel which controls its functions The following explanation of these functions holds equally well for both channels The signals that are to be recorded must be connected to the corresponding input terminals on the rear panel of the recorder The black terminal s are for the negative side s the red terminalls for the positive side s WARNING For personal safety the maximum input voltage across each of the channel terminals should not exceed 30 V AC or 42 V DC Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 13 General Description 14 2 4 8 Sensitivity The input sensitivity of each channel is selected with the range rotary switch which has 14 positions Turned fully counter clockwise a sensitivity of 20 V is attained This means that an input voltage of 20 V will move the pen all the way from the right hand edge to the left hand edge of the paper zero position set to the right side of the paper When the range switch is turned fully clockwise the sensitivity is 1 mV full scale The whole range between these sensitivities is covered
17. h part 15 of the FCC rules emission Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD Accessories and Spare Parts 8 Accessories and Spare Parts Please order accessories and spare parts according to the designation and code numbers given below Designation Code No Pieces Fibre tip pen short nib blue 18 1004 22 5 Fibre tip pen long nib red 18 1004 23 5 Chart paper 20 cm x 25m 18 1001 44 1 Pin connector banana type black 18 1004 24 1 Pin connector banana type red 18 1004 25 1 External control cable 15 pin D sub open wire 15 wires 18 1002 09 1 Mains cord US 19 2447 01 1 Mains cord EU 19 2448 01 T Fuse 250 mA 18 1000 68 5 Fuse 125 mA 19 2367 01 5 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 31 Accessories and Spare Parts 32 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 9 Applications Applications 9 1 Volume axis GE Healthcare pumps HiLoad Pump P 50 and Pump P 1 and HPLC pumps 2150 2249 2248 can be used as an external frequency source for the recorder In this configuration the frequency of the pump stepper motor which is directly proportional to the volume of liquid through the pump regardless of changes in f
18. ical disturbances 3 Electronic fault Consult GE Healthcare representative Clean with cloth DO NOT USE LUBRICANT Consult GE Healthcare representative Consult GE Healthcare representative Reduce AC input voltage Check your mains voltage supply Correct if possible Check your enviroment for electric noise generation Correct if possible Consult GE Healthcare representative Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 27 Trouble shooting 28 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 7 Technical Specifications Technical Specifications Recording channels Input ranges Variable ranges Accuracy of span Non linearity Dead zone Pen response time nput impedance nput nput terminals nput current aximum input voltage Common mode rejection Zero adjustment range Zero position Zero drift Pen lift Chart speed range Chart paper Chart paper life Pens Power supply voltage Power supply frequency Power consumption Environment Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD REC 111 1 channel REC 112 2 channels 1 2 5 10 20 50 100 200 500 mV and 1 2 5 10 20V FSD full scale deflection for each channel 40 10096 FSD 0 3 FSD lt 0 3 FSD lt 0 2 FSD 0 25 1 MO DC Floating asymetrical Low profile banana plugs 5nA Max 60 V between channels and ma
19. in 14 steps according to the sequence 1 2 5 10 20 50 mV and 0 1 0 2 0 5 1 2 5 10 20 V The input sensitivities have these calibrated values only when the cal var key is in the cal position 2 4 9 Calibrated variable span When the key cal var is in the cal position an input signal equal to the selected range will result in a full scale deflection FSD However it is sometimes desirable to have an input span which lies between the values chosen with the range switch In such cases the cal var key must be in the var position after which the variable span can be adjusted by turning the potentiometer with the fingertip or the aid of a small screwdriver Example Suppose a signal varying between 0 and 25 mV has to be recorded and one wants to use the full scale width 1 Select the 50 mV range 2 Put the cal var key in the var position 3 With a 25 mV signal connected to the analogue input adjust the potentiometer until the amplitude reaches 100 of scale Since the variable span allows 40 to 100 span adjustment any desired non calibrated span between 0 4 mV to 25 mV can be obtained 2 4 10 Zero and zero adjust When the zero key is in the down position the pen will remain at its zero location This is the electrical zero of the pen in the absence of an input voltage The location of this point can be shifted to the left or right by turning the zero knob
20. low rate viscosity etc will control the chart drive of the recorder Hence the X axis of the recorder will represent a volume axis It is therefore possible to compare chromatograms which were run at different flow rates and to obtain direct measures of void volumes peak volumes and retention volumes Calibration of the volume axis The signal frequency of the above mentioned pumps is proportional to the volume of liquid displaced by the pump The volume delivered per pulse is dependent upon e the size of tubing used e the condition of the tubing e the pressure applied on the tubing It is therefore necessary to calibrate the pump to determine the volume delivered in relation to the chart speed Proceed as follows 1 Set up the pump recorder and fraction collector 2 Immerse one end of the tubing in a beaker filled with water 3 Connect the other end of the tubing to a 1 ml pipette 4 Setthe chart speed selector to 10 mm min and off position 5 Start the pump and watch the water rise up the tubing 6 When the water level has reached the tip of the measuring pipette make a mark on the chart paper 7 As soon as the water level reaches the 1 ml graduation mark stop the chart drive 8 Measure the chart length in cm and calculate the volume cm as represented by the chart speed 1 ml measure chart length cm 9 The calculated value is valid for the 10 mm min set switch Other chart speeds can be obtained by
21. m which the time functions are drawn Owing to the nature of the dual channel recorder the pen of channel 1 is always 2 5 mm behind the pen of channel 2 Full sensitivity ranges from 1 mV to 20 V DC Recorder REC 112 is equipped with one chart drive control panel and two independent isolated servo systems with a control panel for each channel Recorders REC 111 and REC 112 have an attractive design with much attention having been paid to ergonomics control functions are well positioned keys and switches are very accessible and paper is easily changed The pens which are non smear and disposable give clear accurate traces for instance evaluation Chart paper is of a high quality and 200 mm wide Improved control capabilities permit the use of these recorders in all GE Healthcare chromatography systems Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 7 Introduction 8 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 2 General Description General Description 2 1 Top view Emm 8 16 3 0 TE 215 9 Lee I W ER 10 15 14 11 12 Fig 1 Model REC 112 No Item Description Servo system one for each channel 1 range selector 2 calvar span 3 var adjustment 4 zero 5 zero adjustment 6 pen up down Rotary switch with 14 positions selects span settings ranging from 1 mV to 20 V
22. means of the other chart speed settings For example 0 1 mm min will correspond to 10 mm min _ 100 times the volume cm chart speed 0 1 mm min previously calculated Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 33 Applications 34 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD www gehealthcare com lifescience GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden Drop Design HiLoad and FPLC are trademarks of GE Healthcare companies GE imagination at work and GE monogram are trademarks of General Electric Company All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 2006 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric company GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box
23. mps and LC controllers can be used as the external source for the recorder In this configuration the frequency of the stepper motor which is directly proportional to the flow of liquid through the pump regardless of changes in flow rate viscosity etc will control the chart drive on the recorder Hence the X axis of the recorder will represent a volume axis Therefore it is possible to compare chromatograms which were run at different flow rates and to obtain direct measures of void volumes peak volumes and retention times For further information on how to connect the recorder to the above instruments or GE Healthcare FPLC please refer to the instruction manual for the instrument system concerned or contact your local GE Healthcare representative 3 10 Remote chart ON OFF control of chart drive Remote control may be effected by an external switch which can be connected between pin 15 and 12 on the 15 pin D sub connector Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 19 Installation 20 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 4 Operation Operation When all the necessary input connections have been made the recorder is ready for operation Before switching on the mains all panel keys should be in the up position T WARNING In case of an emergency situation the mains switch must always be easy to access 4 1 Operation in panel mode 1 2
24. ower switch on the left side is in the OFF position Fig 3 27 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 15 Installation 16 2 Check that the mains voltage selector is set to mains voltage of the laboratory Fig 2 23 3 Select the correct fuses from the fuse kit supplied For 100 120 V operation use the 250 mA fuses supplied For 220 240 V operation use the 125 mA fuses supplied Insert the fuse into the fuse cap and then fit the fuse cap into the fuse holder socket on the left side of the instrument Fig 2 28 Discard the unwanted fuses immediately 4 Select and connect the mains cable corresponding to your mains supply Discard the unwanted mains cable immediately WARNING To comply with electrical safety standards the signal voltage should never exceed 30 Volt 3 4 Loading the chart paper roll Unpack the paper roll supplied with the recorder and put into place Fig 4a Ensure the paper roll is positioned so that the printed grid faces the user during normal operation Lift the plastic transparent ruler Fig 4 b pushing it gently upwards so that it rests over the top of the recorder Leave it in this position Pull the paper forward it should be parallel to the recorder housing With your fingertips fit the holes in the edge of the paper over the sprockets Do this on the right hand side first and then on the left hand side Fig 4 c This is important because the holes in the
25. parately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Contents Contents 1 Introduction 2 General Description Ll OR RO 9 2427 Rear Tente ee A ie 11 23 OO Side VEW ee E r en en REOR 12 24 Chart drive CES COT ONT nee een tance 12 2 4 1 Record OON aas oret nes nitas er teen 12 2 4 2 CRON SO COG PEE 12 2 4 3 nterna ekt ore aaea a en ee 13 2 4 4 Paper transpor c erene E E E R AER 13 2 4 5 El eee a E EEE E oe ates 13 2 4 6 GOTO NEXT GLO IER scettr dein ne inne N 13 2 4 7 Servo SUSEelTY s cst tiat etum eed tl ette c ial ee trita D edd 13 2 4 8 SEU 14 2 4 9 Calibrated variablesspam usu ait esce ten co eben 14 2410 2610 Ond zero Ql Sb atero tees datei desee cc ceteri E 14 2411 Sendp dOWLHlauussosause iacente eU eem ananassae 14 Installation 21 MORE ACER Sd CU UM 15 3 2 Safety instructions for power connection 15 mc BEBchical connections sree ie a ne 15 54 Judi die char pps roll aac de 15 ELE Ure cg e taba tt ranisa pisessi ts 17 55 Connecting tbe IUE SINS seccion eere Ent Rt 17 37 uo MCSD meer eee 18 2 9 Ta pne S d Edad quU Su d a CM ERES 19 ti NEU ace ce 19 3 10 Remote chart ON OFF control of chart drive 19 Operation 4l Operation in panel MOTS el 4 2 Operation in external mode by external pulses el 4 5 Home
26. recording 22 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD Maintenance 5 Maintenance GE Healthcare recorders require the minimum of maintenance No lubrication is necessary Never lubricate the guide axis of the pen carriage Clean the guide axis periodically with a soft cloth to remove dirt When changing chart paper remove any excessive dust All servicing should be entrusted to qualified personel only Please contact your local GE Healthcare representative for more service information The following checklist is meant to be a guide in trouble shooting for the most frequent problems If these checks are executed and the recorder still does not work properly consult your local GE Healthcare representative 5 1 Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 23 Maintenance 24 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 6 Trouble shooting Trouble shooting 6 1 General Symptom Cause Remedy Recorder does not work 1 No mains voltage Check 2 Mains switch off Check 3 Mains cord defective Check 4 Fuses blown Check mains voltage settings and fuses
27. right side guarantee exact paper positioning whereas the holes on the left side only support transport When the paper is correctly positioned over both sprocket wheels hold the paper in position and lower the ruler Then with both hands gently apply pressure to both the left and right edge of the ruler A double click indicates that the ruler is properly positioned Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 18 1004 23 18 1004 22 Fig 5a Installation 3 5 Writing system GE Healthcare Recorders REC 111 and REC 112 have a writing system that is very simple and convenient They use reliable fibre tip pens of the disposable cartridge type Figs 5 a and b which leave a very fine trace and do not bleed To install the pens remove the cap and gently push the pen into the plastic pen holder avoiding sidewards or upwards pressure Note To prevent the pens drying out when the recorder is not in use cover the tips of the pens with the caps provided Fig 5b 3 6 Connecting the input signals The input signal is connected to the analogue input terminals on the rear panel by banana plugs Fig 2 17 and or Fig 2 18 The input signals should be connected as follows Red terminal High lead with the highest impedance to ground positive Black terminal Low lead with the lowest impedance to ground negative Metal terminal Ground shield or ground lead The input signal shoul
28. tered Any pulse that is shorter than 40 ms is rejected As a consequence these signals are always delayed by 40 msec When an external chart drive pulse is used to move the paper under the pens a so called dividend is applicable This is a number between 1 and 12 000 by which the number of received pulses is divided before one step is made i e 0 05 mm The value of this dividend depends on the positions of the key mm min or mm s and the rotary switch chart speed Switching from mm s to mm min increases the dividend by a factor of 60 and turning chart speed from 20 towards 0 1 accounts for a factor of 200 The combination of these two results in a maximum dividend of 60 x 200 which equals 12 000 The maximum frequency at which ECP may be sent to the REC 111 112 recorders is 8 kHz Pulse duration at least 1 usec Since the maximum stepping rate corresponding to a maximum chart speed of 20 mm s and a step size of 0 05 mm equals 400 Hz this maximum ECP frequency can only be handled properly by the recorder if a dividend value of at least 25 is applicable The user is responsible for maintaining this lowest value If the stepper motor of the chart drive receives stepping pulses at a frequency greater than 400 Hz it will stop turning regularly This special facility has been built into the REC 111 112 to meet the requirements needed for unusual laboratory applications Under normal circumstances ECP do not n
29. wards with increasing speed 12 feed A Transports the paper backwards with increasing speed 13 set Defines start of plot and major grid mark position 14 home Moves the paper to start of plot position with pen up 15 grid Moves the paper always forward to the next major grid mark lines with 10 mm distance When on grid mark the paper moves one grid mark further 16 power Green LED indicates power on Note The following functions are only available in record off mode 11 feed v 12 feed 4 13 set 14 home 15 grid 10 Record Rec 111 Recorder REC 112 User Manual 18 1132 20 Edition AD 2 2 Rear panel view General Description Fig 2 Model REC 112 No Item Description 17 18 19 20 21 22 23 Analogue input banana plug channel 1 short nib pen Analogue input banana plug channel 3 long nib pen External control Zero position selector Mains voltage selector Chassis ground Mains power inlet The input signal is connected to the terminals on the rear panel y banana plugs Same as above A 15 pin D sub connector for remote control of the recorder 2 position switch used to select left or right hand zero position 2 position switch used to select appropriate voltage range 100 120 V covers 93 5 to 132 V 220 240 V covers 187 to 264 V Earth point to other instruments Inlet for the mains cable Record Rec 111 Recorder REC 112 User Manual 18 1132
30. x 30 V between channel and ground 130 dB 50 to 150 96 of FSD Choice of left or right hand side of chart by a switch on rear panel Right hand zero point on delivery lt 1yuV C Electrical independent control of pen 1 and pen 2 automatic pen lift at power down or after 30 s stop of chart speed 0 1 0 2 0 5 1 2 5 10 20 mm s or mm min 25 m roll according to DIN 16 230 Max 4 167 hours at lowest chart speed Min 20 min at highest chart speed Disposable fibre tip cartridge 115 230 V 50 60 Hz 40W 4 to 40 C 20 95 relative humidity 85 106 kPa 840 1060 mbar atmospheric pressure 29 Technical Specifications 30 Compliance with standards Safety standards EMC standards The declaration of conformity is valid for the instrument only if it is used in laboratory locations used in the same state as it was delivered from GE Healthcare except for alterations described in the User Manual connected to other CE labelled GE Healthcare modules or other products as recommended This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the following harmonized standards EN61010 1 EC 61010 1 e CAN CSA C22 2 No 61010 1 UL61010 1 This device meets the requirements of the EMC Directive 89 336 EEC through the following harmonized standards EN 61326 emission and immunity e EN 55011 GR 2 Class A emission This device complies wit

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