Stains All Gel Staining Kit for Acidic Proteins - B
Contents
1. B Bridge International ino Baa Stains All Gel Staining Kit for Acidic Proteins Catalog PMC AK02 COS For research use only Not for diagnostic use V120613 Table of Contents PETPOCIIGHIONES 8 css inset d ne nace ad ctabs cand aed nt ee Ache aw E Heenan ees oun eee On 3 COMPONGMISs scenes eeee tinct ect eiaee Biseuaiicnc us eevee chadab vee ona cats geud Wau cumeew ses wash Ea 3 Additional Materials ss 22c teens tay eee sa take 2 ed Baris ee es ele ete ela ee 3 Reagent Pr paration nic css eicises cascade ce bia nei wae A E e E AU 3 PROWOCOl ss dorsunccca ciate traks i i rahe tai cate bea a a aa E eCard En TETEE 3 Examples n 2cd2220sceeste oe et ace ene E dovesteaueutsegenurebeiaatoens seeside E ie oeaio eiroet 4 Notice to Purchaser This product is to be used for research purposes only It is not to be used for Drug or Diagnostic purposes not is it intended for Human use B Bridge products may not be resold modified for resale or used to manufacture commercial products without the express written consent of B Bridge International Inc EXCEPT AS OTHERWSIE EXPRESSLY SET FORTH IN THIS USER MANUAL B BRIDGE DOES NOT MAKE ANY REPRESENTATION OR WARRANTIES OR CONDITIONS OF ANY KIND EITHER EXPRESS OR IMPLIED WITH RESPECT TO THE PRODUCTS OR INFORMATION DISCLOSED HEREUNDER INCLUDING BUT NOT LIMTED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY FIT FOR A PARTICULAR PURPOSE OR NONINFRINGEMENT OF THE INTELLECTUAL PROPERTY RIGHTS OF THIR2. D PARTIES 2007 B Bridge International Inc All Rights Reserved B Bridge International Inc V120613 www b bridge com Introduction Proteins that regulate bone mineralization such as osteocalcin osteopontin and BSP Il are abundant in bones and teeth These acidic proteins are difficult to detect by conventional staining methods of SDS PAGE gels Our Stain All kit is specifically designed to stain strongly acidic proteins in SDS PAGE gels The color of the protein band varies depending on the protein s isoelectric point pl and chemical modifications like glycosylation and phosphorylation Components Store kit components at room temperature e Stain Stock 40 ml e Buffer 2 x 200 ml 1 kit can stain 20 mini slab gels Additional Materials May Be Required Pipet Glass dish for staining 25 isopropanol Deionized water Platform shaker Aluminum foil Reagent preparation 1 Stain Solution prepare immediately prior to use a Prepare Stain Solution for 1 gel by diluting 2 ml of Stain Stock with 18 ml of buffer b Protect the Stain Solution from light Protocol Steps to stain 1 mini slab gel 1 Fix the SDS PAGE gel in 20 30 ml of 25 isopropanol Shake for 20 minutes 2 Decant the isopropanol and repeat step 1 Step 1 should be repeated for a total of 4 times to completely remove SDS Alternatively soak the gel overnight in 25 isopropanol Note Any remaining SDS in the gel will react with the Stain Solut
3. ion and interfere with protein staining 3 Decant the isopropanol then soak the gel in deionized water for 10 minutes 3 B Bridge International Inc V120613 www b bridge com 4 Replace with fresh water and repeat step 3 for a total of 4 times 5 Decant water and add 20 ml of prepared Staining Solution 6 Cover dish with aluminum foil to protect from light Shake for 3 hours 7 Decant Stain Solution and wash the gel in deionized water at least 2 times 8 The red color in the background will fade if the gel is left under natural lighting sunlight for approximately 10 minutes The protein bands of various staining intensities and colors will become more visible Example De ad ie y ni a me 8 re ne _ at oan ne ne eo me nei a _ meum n ns a M Col Coll BSA HM OPN BSPII MGP M Col Col BSA HM OPN BSP II MGP CBB Stain All M Molecular weight marker HM Human milk total protein Co Type collagen OPN Human milk osteopontin Co Il Type II collagen BSP Il Bovine bone sialoprotein BSA Bovine serum albumin MGP Bovine matrix Gla protein For assistance and ordering please contact B Bridge International Inc 20813 Stevens Creek Blvd Suite 200 Cupertino CA 95014 USA Tel 408 252 6200 Fax 408 252 6220 Email customersupport b bridge com www b bridge com B Bridge International Inc V120613 www b bridge com
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