CrysCamTM User`s Guide
Contents
1. Q E D LIFE SCIENCE DISCOVERIES PRESS TM rs CrysCam User s Guide 4 Q NY s q e d life science discoveries inc 6487 Half Dome Court Livermore CA 94551 Phone 925 208 74297 Fax 801 880 3982 Table of Contents HASTA ATION rc nee 1 Post and base plate assembly 0 ceee cece eect eeeeeeeeeeeeeeeeeees 1 Initial camera assembly 2 Camera driver installation 3 J0IWareIBS1AlAtIGN mm 5 Imaging with CrysCam eee ol 7 Taking OCMC S n RS EAER nET EEEE 7 How to record a time lapse video 8 Using the DOlANZBF 2 ne 9 Using the base plate inserts 10 Base plate electronics wiccisccisiscteisdessicisessctetevs sherk 10 LED replacement Warranty and replacement parts 12 CRYSCAM MANUAL V 1 1 Chapter Installation Thank you for buying a CrysCam Please follow the unpacking instructions in the shipping carton and make sure you have found and identified all components Post and base plate assembly Post The 3 4 inch stainless steel post needs to be screwed tightly into the base plate The collar is already adjusted so that even at the lowest focus settings the optics will not crash into a Linbro type plate If you need to adjust the collar for some reason a 1 8 hex wrench is included in the spare parts bag Base Plate The microscope base plate is preassembled with the LED driv2. Capture file location The default capture file location is hidden deeply in the My Documents subfolders which is probably not practical To reset the capture file directory open File Album and right click the Capture Folder to select a new default directory Ej Album Capture Files to c data snaps motic EN Capture Folder Photo Folder C Biology C Industry C video C Quicken robohelp 5 Roxio 6 Sexie32 i snaps Ons Teaching TurboTax Visual Studio Pre xprog DELL Documents and Seti DRIVERS M rane 4 Gas gt Folder Amalgamation lysopol jpg lysopol2 jpg lysozyme_40_mg_ml jpg toplight jpa wholescreen jpa Capture directory selection in the File Album menu CRYSCAM MANUAL V 1 1 Chapter Imaging with CrysCam Imaging with the CrysCam is easy and intuitive After learning how to take and save pictures explore the other software options as described in the software manual and on the softvare CD Taking pictures Assuming that you have properly installed the camera as described above open the Motic Images software and make sure the proper camera type in File Setting is selected if not yet set to MC1001 2001 Next click the live capture icon the small movie camera and the live capture window appears Any error message would indicate that the drivers are not properly installed At this point a fuzzy image frame should be visible U
3. drag them into the storyboard You can select title images transitions etc from corresponding menus and remove uninteresting leading or trailing frames If growth slows you can also remove every other frame or more if necessary Preview the movie and save it Using the polarizer The polarizing adapter is attached to the optics assembly with 3 thumb screws NEVER OVERTIGHTEN ANY OF THE SMALL THUMB SCREWS The rotating polarizer itself is screwed into an adapter ring which connects to the threaded insert of the lens adapter secured by another thumb screw You must use the linear polarizing insert above the thin frosted acrylic insert in the base plate can be used with or without the frosted diffuser but the polarizer must be on top of the diffuser disk Adjust the 3 set screws so that the polarizer is slightly lower than the base to avid scratching the coating Turn the polarizer on the lens adapter until you achieve the desired effect The polarizers are high quality linear type and allow full extinction when crossed for great dark field pictures See sample images onthe qedlife website CRYSCAM MANUAL V 1 1 Using the base plate inserts Lighting Base Inserts The base plate has a 25 3 mm recess that can accept a variety of inserts The rim of the opening has 3 set screws that allow adjustment of the inserts so that they level with the surface of the plate A small hex 1 16 wrench and extra set screws are included in the spare p
4. arranty We ship cheap spare modules in that case e Camera 90 days A CMOS color camera can be destroyed by a variety of hostile actions like poking into the chip zapping a laser into it dropping it tapping on it with hard objects or using it as a missile All of the above and even remotely associated actions including detaching it from the optics void the warranty There is UV fluorescent Loktite on all optics threads A few weak pixels visible at extremely low lighting are normal for those CMOS chips e Base post and Z drive One year warranty These sturdy metal pieces can only be destroyed by willful abuse which voids the the warranty in any case e Image capture software 30 days original manufacturer warranty If any of these warranty claims apply to you please mail us sales qedlife com or call the US phone number 925 209 7429 9 00 to 17 00 PST Due to technological advances changes design details are possible without explicit notice q e d life science discoveries inc Attn CrysCam 6487 Half Dome Court Livermore CA 94551 USA 925 209 7429 Downloads http ruppweb dyndns org cryscam http www gedlife com
5. art bag The recess is originally adjusted for the frosted acrylic diffuser plate and the polarizer in standard and advanced systems and the frosted insert only in systems without polarizer Make sure the polarizer is placed ABOVE the frosted insert For use with top lighting only the black Delrin insert can be used You can use any combination of inserts to achieve optimal lighting for your purpose even a small mirror or any 25mm insert of your own design Use the set screws to adjust the inserts to be slightly lower and level with the base plate Base plate electronics The base plate circuit board provides the option of adjusting the maximal brightness of the LEDs This may be desirable in certain settings for limiting overexposure or to extend LED life Turn the lighting knob on the base plate to max intensity Then insert a non metallic small screw driver in the hole in the PCB and adjust the potentiometer until the desired max brightness is set Plug for AC e adapter Access to max brightness potentiometer CRYSCAM MANUAL V 1 1 The entire electronics assembly can be replaced if necessary by unplugging the brightness control connector the battery case and loosening the 4 pan head screws Then the LEDs can be replaced as follows LED replacement The LEDs can be exchanged by removing the PCB from the base plate and pulling the LEDs from their sockets The long or red painted leg of spare LEDs is opposite the flat sp
6. er board and the battery attached The sorbothane cushioning feet are attached with double sided tape which can be easily replaced should the feet ever come loose There is enough clearance of the cushioned PCB board and the battery case if the base is placed on a clean and level surface Make sure there are no protruding parts on the working surface that could damage the board when you set down the microscope base Battery and Power Supply The battery case located below the base houses 4 AA batteries and is attached with Velcro tape To replace the batteries carefully detach the lead connector from the battery case pull the battery case off and install new batteries The AC power adapter connector is located directly on the LED board and faces towards the steel post Both the AC adapter and batteries can be used at the same time the AC adapter then overrides the batteries Brightness Control The LED board has a variable lighting circuitry which is controlled by the knob on the top right of the camera The potentiometer also has a switch that turns the power off Make sure to turn the knob all the way to the left until it clicks and the LEDS switch completely off Should a LED fail the diodes can be replaced if necessary described later CRYSCAM MANUAL V 1 1 Lighting Base Inserts The base plate has a 25 3 mm recess that can accept any variety of inserts The rim of the opening has 3 set screws that allow adjustment of the inserts so tha
7. he driver installation will continue until finished Proceed to the software installation CRYSCAM MANUAL V 1 1 Software installation Software Installation Double click your CD drive icon and the software installation wizard will open Follow the instructions details are provided in the separate software installation manual If the install wizard does not open run setup from the CD The serial number of the software is printed on a label on the CD case IMPORTANT Select the correct camera type in File Settings or the and select Setting software will not work Optional Twain Driver Installation Twain compliant drivers are available from the q e d website and from Motic USA directly You need those drivers only if you wish to use the camera directly from other Twain compliant software such as Photoshop or Acrobat or if you wish to write your own Settings Capture Music InterFace File Name Capture Serial number Auto Capture Every 30 Seconds v Capture one image Maximum Capture images 30 Image Size Auto z F Using current date and time as file names r Module Setting Capture Source Select mMc1001 MC200 You must select the MC1001 2001 camera in the File Setting menu interface Camera selection Select the correct camera type or the software will not work Open the Motic Images software by double clicking the program icon Pull down the File menu CRYSCAM MANUAL V 1 1
8. ire camera and optics assembly can be used to fine adjust the optics so that they are perfectly centered above the base light source center of the light source using the live image In case you need to remove the camera from the rack and pinion drive it is necessary to first detach the polarizing adapter by loosening the 3 thumb screws which secure the polarizer adapter to the optics Then the whole optics assembly can be removed from the rack and pinion drive by loosening the small thumb screw on the rack and pinion drive to release the camera NEVER OVERTIGHTEN ANY OF THE SMALL THUMB SCREWS The smallest of the 3 hex wrenches 1 5 mm fits the set screws used to hold the components of the optical assembly together and to secure the large 50 mm adapter ring to the optical tube Exercise care and do not over tighten the fine threads Cleaning the optics Use can duster available at office supply stores to remove lint and dust and never rub the linear polarizer the film coating is sensitive Use only soft optical lens cloth CRYSCAM MANUAL V 1 1 Camera driver installation Computer Requirements Laptop or Desktop Windows XP SP2 Pentium 4 1 GHz or above processor or AMD Athlon 2600 XP 256 MB RAM 400 MB free disk space USB 2 0 32 MB video card and CD ROM drive For some desktop computers you will need to install the USB 2 0 card as your computer may not be able to supply sufficient power to drive the camera Desktop Comp
9. o Compression Language Settings English Mi gt Il live Ki Note Note that the live video image destination directory but not the capture directory is set with the Video selection box short red arrow How to record a time lapse video See sample movies on the Using the CrysCam and the Windows MovieMaker comes with Windows XP you can actually make crystallization movies First get an estimate of how long it will take to qediiie website grow your crystals Then decide how long the movie should be The fastest setting in Movie Maker is 8 frames second Example You want to make a 20 second movie You need thus 160 frames Assume that the crystals grow in 12 hrs Thus we need about 13 frames per hr or one frame every 4 5 minutes At the minimum capture resolution 320x240 a frame is about 10 13 kB in WMV format Thus our movie would be 1 6 2 MB a reasonable size for web streaming Set the capture options accordingly in the File Settings window optimize the image and then click Autocapture in the capture window After 12 hrs the 160 images should be captured in the directory you selected in the Video box of the capture window CRYSCAM MANUAL V 1 1 Open Windows MovieMaker Go to Tools Options and select the image duration time to lowest available 0 125 sec Close with ok Then drag or load File all images in the image collection panel select all and
10. ot on the LED case The board clearly indicates the LED position for proper installation Cut the leads to about 3 mm length past the stop use the old LEDs as a sample Polarity is critical for function but inserting the LEDs the wrong way will not destroy the board The standard LEDs are warm white with tint type D Additional spare LEDs boards and parts are available from q e d labs LED sockets See text for proper replacement procedure CRYSCAM MANUAL V 1 1 Warranty and replacement parts There are no user serviceable components in this practically indestructible instrument Each instrument is run for 24 hrs and a set of calibration images is recorded with it during quality assurance testing If something breaks or you cannot install the software its is probably because you did not follow the instructions Please read the instructions all of it in the right sequence If you still hate it send the instrument back at your cost properly packed no we do not insist on original packaging for a full refund within 10 days No questions asked e Optics 90 days warranty If it breaks you must have dropped it stepped on it or thrown it at somebody all of which voids the warranty e Lighting 30 days That is about the time it takes until someone figures out a way to improve the circuit power it with solar cells replace the LEDs with light bulbs or put the microscope into an acid filled sink All of which voids the w
11. se any crystal trays or the calibration slide in the camera accessories box to find the focus and zoom settings Note the enhance click box and use the auto adjust feature to optimize exposure Adjust the lighting so that you get reasonable gain and exposure settings To work with Selecting capture and screen resolution blue maximum capture arrow For high quality images high capture settings are resolution when ok but be aware that live and time lapse video clips higher than the become huge if you select to high capture resolution For screen resolution capture resolutions exceeding the available screen center the slides resolution click Fit to Window or adjust the sliders as yellow arrows so described in the box that the image remains centered You can select the file names and image file formats when you zoom from the settings menu The File Settings menu figure above is also used to select the time lapse and total number of frames for the timed capture to be selected CRYSCAM MANUAL V 1 1 Size 1280x1024 M 640 x 480 v Play ne Gain g Fit To Window Keep Ratio amp Capture AutoCap Video Help White Balance Cancel ROI Software Gain Color R C G C B C 1 OFip Menhance Background Calibration Balance Enabled Calibration Video C data snaps motic V Use Vide
12. t they level with the surface of the plate A small hex wrench and extra set screws are included in the spare part bag The recess is originally adjusted for the thin frosted acrylic diffuser plate and the polarizer insert together in standard and advanced systems and the frosted insert only in systems without polarizer Make sure the polarizer is placed ABOVE the frosted insert For use with top lighting only the black Delrin insert can be used You can use any combination of inserts to achieve optimal lighting for your purpose even a small mirror or any 25mm insert of your own design Use the set screws to adjust the inserts to be slightly lower and level with the base plate Initial camera assembly Mounting the Camera and Optics The camera optics and polarizer are preassembled in the rack and pinion z drive focus mount which slides onto the steel post Note that the camera cable needs to extend towards the observer away from the post for proper orientation of the image on the computer screen After installation Adjusting the Rack and Pinion Drive The rack and of the software pinion mount needs to be secured so that the polarizer is fine adjust the approximately 50 mm above the drops 80mm on the camera abovethe advanced system with the large set screw in the back of the mount Align the optics above the lighting port Once the software has been installed the small set screw on the rack and pinion focus drive that secures the ent
13. uter If you have a desktop computer with low power USB ports that cannot drive the camera you may need the optional PCI USB 2 board Please follow the separate instructions included in the box with the board and drivers USB1 0 DOES NOT WORK Windows XP Driver installation Plug the camera in the USB2 port of your laptop The following window will appear Found New Hardware Wizard Welcome to the Found New Hardware Wizard This wizard helps you install software for Motic USB2 0 If your hardware came with an installation CD lt lt or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue Insert the CD before you click next The CD will load Ignore the following warning and click continue anyway CRYSCAM MANUAL V 1 1 Hardware Installation The software you are installing for this hardware Motic MC Camera 2 has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway STOP Installation T
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