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Removing the MP-520V Pump Unit

1. 2 D 8 MP 822V D UT 7158 d PRESSURE SENSOR E12 NS H 14 p er ee E E EEEE ama na aaa 1 a TRE a 10 13 ey TT T b WA
2. nen 2 8 Check Procedure edi n td rio Ree Lc ov eg de 2 8 CNW Data GME CK cm 2 9 Check Items Before Instrument 2 10 Check Items before Instrument 2 10 Sampled Whole Blood Handling 2 11 Blood Sample Handling for Counting 2 11 Blood Sample Handling for High 2 11 Blood Sample from a Patient with Specific Conditions 2 11 Incorrect Dates E 2 12 Background 2 12 How to Decrease the Background Noise the Instrument after Long Term Storage iconem 2 13 Laser Optical Unit Problems sssssssssesseeeeeeeeeeeeneeen nennen nnns 2 14 ProD Fassina EE 2 14 PODIO Zoossen S 2 14 Scattergram Problems 2 0 1000 msnada kie riitaisa nnne sens 2 15 Possible Causes and Countermeasures for Poor WBC Reproducibility 2 27 Treubleshooting am ALAM sa E 2 28 Reagent Alam ELE 2 28 Board Unit Description gt 4 3C 1 SUD Bath UNIU 2 3 1 CBC
3. 5 1 RS 2826 SOCKOL Tes duda 5 1 820 50 E 5 1 USB SOCK 5 1 Service Manual MEK 8222 56 1 5 SOCKET PIN ASSIGNMENT Socket Pin Assignment RS 232C Socket 6 000000000000 ZK 820V Socket 2 USB Socket om Service Manual MEK 8222 CAUTION Connect only the specified instruments to the connectors or sockets marked with by following the specified procedure Otherwise electrical leakage current may harm the operator D sub 25 pin female SDBB 25S SL LNK 02 Pin No Signal Pin No Signal Pin No Signal 1 NC 10 NC 19 NC 2 TXDI 11 NC 20 To pin 6 3 RXDI 12 NC 21 NC 4 RTSI 13 NC 22 NC 5 CTSI 14 NC 23 NC 6 To pin 20 15 NC 24 NC d SG 16 NC 25 NC 8 NC 17 NC 9 NC 18 NC Pin No Signal Pin No Signal Pin No Signal FG 4 To pin 6 7 CTSO 2 TXDO 5 SG 8 RTSO 3 RXDO 6 To pin 4 9 VCC Pin No Signal 1 NC 2 USB D 3 USB D 4 ED 5 1 Section 6 Adjustment Adjusting the Flow POoSIIOE 6 1 Adjusting the FS FL SD Gain and FS Threshold 6 4 Adjusting Sensor
4. 1 24 Connection and Wiring Diagram nennen nnne nnns 1 27 Hydraulic System Diagram iiia ce 1 28 Uc 1 29 Service Manual MEK 8222 1C 1 Introduction Service Manual MEK 8222 1 GENERAL CAUTION To maintain the instrument in normal condition the user must perform the periodic maintenance Refer to Maintenance of the operator s manual This tentative service manual consists of the General and Socket Pin Assignment sections only NOTE If the instrument has a problem and there has been no periodic maintenance the instrument will usually be normal again by cleaning the fluid paths or replacing a consumable with a new one 1 GENERAL Service Policy 1 2 CAUTION e Be careful not to directly touch any place where blood is or may spread to e Wear rubber gloves to protect yourself from infection before doing maintenance Nihon Kohden Corporation s basic policy for technical service is to replace faulty units printed circuit boards or parts We do not support component level repair of boards and units outside the factory NOTE e When ordering parts or accessories from your nearest Nihon Kohden Corporation s distributor please quote the NK code number and part name which is listed in this service manual and the name or model of the unit in which the r
5. BD AO AD Model Number Remarks YK CO AO eal a am ofa 532639 lt MF 820V gt 7 5 m tube AO Index Model Number Remarks lt ZY 820V gt AO 1 YK AC 470 532639 200 200 Index Model Number Remarks 1 2 3 532639 4 532612 Standard Accessory 30 7 5 m tube 7 5 m tube Silicon tube 0 5 x 2 3 AE CD Tube for HEMOLYNAC3 400 mm long AC AD Tube for HEMOLYNACS 400 mm long Note To use 7 5 m tube or 100 m tube cut it in the optimal length TWHANAD Section 2 Troubleshooting Check Pro edure e C 2 1 Background Noise Check 2 2 Measuring Background 04 4 2 2 M Lm 2 2 EN 2 3 Scalttergram CHECK dicente enter 2 4 Counting Blood Samples of a Healthy 2 4 Reproducibility Chek sarisi reirse 2 8 Check Procedure EENEI 2 8 CV CheCK ssis mpna E EAEE 2 9 Check Items Before Instrument Check sss enne nnns 2 10 Check Items before Instrument
6. 6 10 HOB SOMSON 6 11 HGB sensor adjustment n nnnc odii canine 6 11 Calibration 6 12 SENSO 6 13 4 channel liquid sensor unit 6 15 Pressure Sensor esent nennen seen nnns 6 19 Temperature Sensor cree Rue AER RR 6 20 Setting on the UT 7159 WARMER CONTROL board 6 21 Calibrating the Touch Screen nnne 6 22 Service Manual MEK 8222 RELATED CAUTION This equipment and or system complies with the International Standard EN61326 1 for electromagnetic compatibility for medical electrical equipment and or system However an electromagnetic environment that exceeds the limits or levels stipulated in the EN61326 1 can cause harmful interference to the equipment and or system or cause the equipment and or system to fail to perform its intended function or degrade its intended performance Therefore during the operation of the equipment and or system if there is any undesired deviation from its intended operational performance you must avoid identify and resolve the adverse electromagnetic effect before continuing to use the equipme
7. 8222J 8222 AUTOMATED HEMATOLOGY ANALYZER MEK 8222 0634 001851A Model MEK 8222J K Manual code 0634 001851A Reader Comment Card We welcome your comments about this manual Your comments and suggestions help us improve our manuals Please circle the number for each of the following statements corresponding to your evaluation and add comments in the space provided Fax or send your completed comment card to Fax 81 3 5996 8100 International Div Sales Promotion Section Nihon Kohden Corp 1 31 4 Nishiochiai Shinjuku ku Tokyo 161 8560 Japan Strongly Agree Neutral Disagree Strongly Agree Disagree This manual is organized 1 2 3 4 5 can find the information want 1 2 3 4 5 The information is accurate 1 2 3 4 5 can understand the instructions 1 2 3 4 5 The illustrations are appropriate and helpful 1 2 3 4 5 The manual length is appropriate 1 2 3 4 5 Comments Thank you for your cooperation We appreciate it very much Name Occupation Position Hospital Company Address Phone 5 Contents EMC uerum S i Conventions Used in this Manual and iii Warnings Cautions and 0 0 0 1 00
8. WA 460V Option Thermal Printer WA 820V Option VcVNO eVcvNO CNA217 CNA241 5 232 RS 232C DSUB 9 DSUB 25P 216 113 Sheath pump unit MP 822V Laser optical unit MO 820V CNA305 CNA304 CN201 HKP6P CNP306 4 Channel liquid sensor unit JQ 823V CN202 CN203 Inlet outlet unit JQ 820V Center piping unit 02 PHD16P cnoo1 VH5P HIF 60P Display unit PV 820V Boe PHD12P 0 211 D Ne CNA105 d 28 PHD28P CN101 conn CN202 CNP207 CNA103 MC 821V Flow cytometry piping unit Sample pump unit MP 821V UDO Upper Position Lower Position UD1 Service Manual MEK 8222 Pump unit MP 520V 5 part WBC Position Sensor SEN E vHsP HIF 60P PHD20P 3 CN102 VH6P HIF40P CNA205 204 CNP238 CNA202 0202 AMP8P CN201 MFC10P CNA240 auci ME CNA104 CN301 PHD10P CNA101 PHKGP cnoi VH10P HD10P CNA301 Closed sampler unit MS 820V BPOS SENSOR board UT 7185 Position Sensor POS Sampling nozzle Position Sens
9. s fL Granularity lt Fig 6 gt Since the blood has too many WBC and NE the NE is widely scattered on the Size Complexity scattergram 2 20 Service Manual MEK 8222 e Scattergram 4 2 TROUBLESHOOTING The following example shows that the 5 parts of WBC are quite differently scattered from the normal one or not displayed at all Possible Causes Countermeasure Specific blood characteristics When there are too many ghosts for the instrument to count the WBC the scattergram shows only the ghosts High membrane resistance of the red blood cells collected from the patient who has a disease such as liver disease or has red blood cells resistant to hemolyzation allows such a scattergram See Fig 13 The blood sample of a leukemia patient has no normal scattergram See Fig 7A 7B Perform measurement method such as the smear method instead of using the hematology analyzer Wrong gain setting for the scattered light pulse If the amplifier gain for scattered light is set excessively high or low most of the white blood cells are shown at the end of the scattergram See Fig 8 Adjust the amplifier gain optimally Refer to Section 6 Adjusting FS FL SD Gain and FS Threshold Wrong reagent use If a diluent other than ISOTONACe3 or hemolysing reagent other than 5 is used the scattergram has data reliability Use Nihon K
10. 2 10 Sampled Whole Blood Handling 2 11 Blood Sample Handling for Counting 2 11 Blood Sample Handling for High 2 11 Blood Sample from a Patient with Specific Conditions 2 11 Incorrect C E 2 12 Background oi ORUM 2 12 How to Decrease the Background Noise on the Instrument after Long Term Storage sssssssssssssesseeeeeneneennee enne nnne nnns nnns 2 13 Laser Optical Unit Problems o pne A EL dk a rd 2 14 9 6 e 2 14 wie E 2 14 Scatt rgram Problems B nem er te ten needa aed 2 15 Possible Causes and Countermeasures for Poor WBC Reproducibility 2 27 Troubleshooting eroe Ember tma cea 2 28 Reagen var addc enda E pest dv ER upra Ea YR 2 28 Service Manual MEK 8222 2C 1 Check Procedure Service Manual MEK 8222 Check the instrument according to the check procedure below Laser switch check s the laser switch turned on pa Environmental condition check s there enough reagent for measurement Are the correct or recommended reagents used s the operating temperature acceptable s the
11. 10 5 32 MCHC 33 LINO ABNORMAL 1233 182441 1123 185 uL L EE 5 g dL 183 ttL hrombocytosts WBC RBC HGB HCT MCU MCH MCHC PLT RDW PCT MPU PDW MEKSDN BC RBC 7 4 5 23 15 8 47 3 90 4 30 2 33 4 242 11 4 9 23 9 5 19 8 10 uL 185 uL g dL k fL g dL 109 uL L H 2 23 2 TROUBLESHOOTING Q7 FEB 01 15 18 W7 FEB 01 ERR 0005 188344 WBC 6 9 10 uL RBC 6 42H 10 4 HGB 16 0 g dL HCT 58 3 98 8 fL MCH 24 42 MCHC 27 4 g dL PLT 286 10 uL Comp lex ty Granular ity RDW 12 3 PCT 29 MPU 18 4H PDW 20 0H Granularity lt Fig 10 gt Failure of diluent or blood sample aspiration causes the overflow of the diluent or blood from the sampling cup to go in the RBC measurement bath It makes the RBC HCT and PLT higher and displays abnormal erythrocyte index message flag such as Hypochromia 9 MAR B 12 88 Q9 MAR IDNO NOSAMPLE 0001 18444 WBC 7 0 10 uL RBC 5 56H 108 01 HGB 15 9 g dL HCT 49 8 x MCU 88 7 fL 28 6 MCHC 32 3 g dL PLT 244 10 uL Comp lex ty Granularity RDW 12 0 PCT 24 PU 10 0 RB PDW 19 9 Granularity lt Fig 11 gt 2 24 Service Manual MEK 8222 Service Manual MEK 8222 2 TROUBLESHOOTING 7 FEB 1 13 45 Q7 FEB 1 IDNO LESEROFF 0001 4 WBC RBC HGB HCT MCU MCH PLT Com
12. 40H 02 298 5 MCHC 320 g dL x PLT 204 10 uL Comp lexi ty Granularity RDW 15 0 33 MPV 162 fL PDW 19 7H 5 RB 5 PLT L 5 5 1 Granularity 13a 289 239 97 2 Excessive low threshold gain 02 FEB 99 13 15 02 FEB 99 IDNO 000000000004 96 10 1 WBC 47 10 NE 46 8 22 RBC 499 109 LY 322 1 5 HGB 148 g dL MO 48 0 2 HCT 46 7 EO 129H 0 6 MCV 93 6 fL BA 33H 02 297 pg MCHC 31 7 g dL PLT 196 103 uL N V RDW 11 5 0 30 MPV 156H fL PDW 190H Comp lexi ty Granular ity NEM 7 5 RB 5 PLT 5 5 2 A Granularity 150 7200 2710720 2 6 8222 2 TROUBLESHOOTING The scattergram shown below results from one of the following reasons The flow cell unit position is wrong or the flow cell is clogged e The blood sample has a problem e Problem such as leakage is somewhere in the fluid path OXFEB 99 13 15 O2 FEB 99 000000000004 9 101 WBC 5 2 103 uL 49 7 25 5 17 1094 37 5 20 150 5 sc MO 50 0 3 HCT 461 i i EO 78H 04 MCV 892 fL 21 00 00 290 MCHC 325 g dL TCR PLT 196 103 uL Comp lexi ty Granularity RDW 11 9 PCT 028 MPV 14 6H fL S RB 5 PDW 178 5 5 2 6
13. value RDW is determined from RBC value Therefore the limitations for RBC also affect RDW value Agglutinated RBC Agglutinated RBC may decrease RBC count and erroneous RDW This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Nutritional deficiency or blood transfusion Iron and or cobalamin and or folate deficiency may increase RDW Very small fragments Very small RBC RBC fragments and WBC fragments may be the cause in increased PLT count Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting in decrease in PLT This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Very large PLT Large PLT may exceed the PLT threshold and may not be counted which results in low PLT count Chemotherapy Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT count In such a case measure manually Hemolysis Hemolyzed specimens contain red cell stroma which may increase PLT count Anticoagulated blood Blood anticoagulated with acid citrate dextrose may have clumped PLT which may cause decrease in PLT count Agglutinated PLT Clumped PLT may decrease PLT count and or increase WBC count For such sample collect the sample in sodium citrate anticoagulant and measure only PLT The PLT result must be corrected for the sodium citrate dilution effect Very large PLT Large PLT may exceed the PLT threshold and not be counted
14. CLOSED CAL ID 0001 WEASUREDGCALIBRATON ASSAY VALUE COEFFICIENT UE VALI eoo 389 1050 30 4 o v C AUTO CAL MODE y Enter the assay value AA 6 AUTO CAL SET Service Manual MEK 8222 1 19 1 GENERAL 5 Manual calibration procedure Measure the MEK 3DN in the closed mode at least 3 times Y The service maintenance menu is convenient for the manual calibration and reproducibility check v v CLOSED n paul 10 data X CV key displays the last ll mes 10 samples average and CV in closed mode MENU C MENU 3 v v C OTHER OPERATIONS C OTHER OPERATIONS v v 4 SERVICE MAINTENANCE SERVICE MAINTENANCE v v CONTINUOUS MEASUREMENT C 10 data X CV v CLOSED 10 SAMPLES y Calculate the new calibration coefficient For example N librati ffitient RBC previous calibration coefficient 998 ew calibra london itien assay value 462 peu ates 7 Mean measured value 440 Previous calibration coefficient 5 new calibration coeffitient 998 x 462 440 Assay value Mean measured value 1048 p p v CBC calibration is complete v The calibration of
15. 4 6 Removing the MS 821V Open Sampler Unit 4 7 Removing the MS 822V Table 0 0 2 00 00000000 00 00 nnne 4 8 Removing the MO 820V Laser Optical 4 9 Removing the MP 821V Sample Pump 4 10 Removing the MP 822V Sheath Pump 4 11 Removing the MC 821V Flow Cytometry Piping 2 2 44 4 6 4 12 Removing the MP 520V Pump 4 14 Service Manual MEK 8222 4C 1 4 DISASSEMBLY AND ASSEMBLY Before disassembling the instrument according to the following procedures remove the front top side and rear covers from the instrument Removing the PV 820V Display Unit The display unit consists of the following Color TFT LCD Touch screen RACK KEY board MANUAL KEY board LCD CONTROL board 1 Remove the 7 screws which secure the display unit to the chassis 2 Slightly push the display unit toward the rear and unhook its inside hook Slowly remove the display unit from the instrument until you can disconnect the 2 cables connected to the MASTER board at the LCD CONTROL board 3 Remove the 3 ferrite cores from the display unit by pressing both hooks of each ferrite core inward Disconnect the 2 cables from the display unit and completely remove it from the instrument Service Manual MEK 8222 Front view 4 1 4 DISASSEMBLY AND ASSEMBLY Removing the M
16. 1 8 Right Side 2 22 2 0 00 00 a Eae nnn sen nnns rennen nen 1 9 Rear E err rere ree 1 10 Inside PANGS inea i 111 RC 1 12 Installation Flow 1 15 How to Upgrade the SOftWare etoile tee are exa deux nose edd 1 21 Replacing the ROM E Rua pu 1 21 Upgrading the Software with the Memory 1 24 Connection and Wiring Diagram sssssssssesesseeeeeenn nennen nennen nnnn nennen nnns 1 27 Hydraulic Systeri DIagratn ue uertit rene au ee oh deua rex nba redux Dn SERERE RR RES 1 28 EI MECU 1 29 Service Manual MEK 8222 C1 5 Section 2 Section 3 C 2 Troubleshooting 2C 1 Check Procedure renee 2 1 Background Noise CHECK 2 2 Measuring Background 2 2 Measurement 2 2 TR 2 3 Scattergram tex tna ER ere bere 2 4 Counting Blood Samples of a Healthy Person 2 24 Reproducibility
17. WBC UP 0 85 V 214 V DETERGENT WBCLOW 046V 0 26 V HEMOLYNAC3 0 23 V RBC UP 0 91 V HEMOLYNAC5 0 21 V RBCLOW 0 23 V CALIBRATION SWITCH SHEATH DILUENT 0 20 V MP 820V LIQUID TANK 472 V CBC ELECTRODE MC 820V SHEATH TANK 415 V WBC WBC 17 9 V RBC SHEATH PUMP RBC 17 9 V 1st PRESSURE 155 kPa 2nd PRESSURE 101 kPa WARMER TEMP 29 C INTERNAL TEMP 33 C Output control 2 6 16 Service Manual MEK 8222 Service Manual MEK 8222 6 ADJUSTMENT 4 Adjust the HEMOLYNAC 5 sensor as follows When there is no HEMOLYNAC 5 in the HEMOLYNAC 5 sensor block adjust the HEMOLYNAC 5 sensor output control 3 until the value indicates 3 5 V or more When the HEMOLYNAC 5 sensor block is filled with HEMOLYNAC 5 adjust the HEMOLYNAC 5 sensor output control 3 until the value indicates 1 5 V or less a SENSOR MONITOR CBC MANOMETER WBC UP 0 85 V WBCLOW 046 RBC UP 0 91V RBC LOW 0 23 V CBC ELECTRODE WBC 17 9 V RBC 17 9 V HGB SENSOR 244 V 0 26 V CALIBRATION SWITCH MP 820V MC 820V WBC RBC ss CBG Diff LIQUID SENSOR DETERGENT HEMOLYNAC 3 HEMOLYNAC 5 SHEATH DILUENT LIQUID TANK SHEATH TANK SHEATH PUMP 1st PRESSURE 2nd PRESSURE WARMER TEMP INTERNAL TEMP HEMOLYNAC 5 sensor 0 21 V 0 20 472V 415 V 155 101 29 33 C Output control 3 6 17 6 ADJUSTMENT 5 Adjust the SHEATH DILUENT sensor as follows a When there is
18. only for part of the equipment HEMO 5 HEMOLYNAC 5 hemolysing reagent Off only for part of the equipment WASTE Laser ON m Hand positions for carrying the e Reset IOIOI RS 232C socket Manual USB socket y Clean IVD conformity AA Eject m lt p Start Disconnection from the mains V xiergeney AC power on Connection to the mains Do not touch the sample rack during measurement Alternating current Inlet Equipotential terminal Outlet Year of manufacture Attention consult operator s manual el e ol tgis Serial number ISOTONACe3 diluent CLEANAC detergent CLEANACe3 detergent oN The mark is protected conformity mark of the European Community The products herewith comply with the requirements of the IVD Directive 98 79 Service Manual MEK 8222 On screen and recorded data Symbol Description Symbol Description Beside WBC or RBC measured value Sample error Beside HGB measured value Dirty measurement baths Beside HGB measured value HGB circuit error Beside WBC measured value Poor hemolyzation Beside HGB measured value HGB voltage adjustment error Beside WBC or PLT measured value Platelet coagulation Service Manual MEK 8222 Section 1 General prote P
19. 100 200 27 79 20 The above scattergram requires adjustment or component replacement Refer to Scattergram Problems section Service Manual MEK 8222 2 7 2 TROUBLESHOOTING Reproducibility Check Use blood sample of a healthy person for 10 measurements The blood sample must be within 8 hours after the blood sample is collected so that you can check the data reproducibility with CV values If the CV values are out of the specified range the instrument does not work normally Refer to Installation Flowchart CLOSED 10 SAMPLES and 10 data X CV on page 1 20 Check Procedure 1 Reduce the background noise so that the scattergram count is less than or equal to 100 Refer to Background Noise Check on page 2 2 2 Prepare blood sample of a healthy person The blood sample must be within 8 hours after the blood samples are collected 3 Turn the sample tube containing the blood sample upside down ten times or more so that the plasma and red blood cells are evenly mixed 4 Setthe sample tube containing the blood sample in tube stand No 1 5 Press the following keys on each screen to display the CLOSED 10 SAMPLES screen MENU key OTHER OPERATIONS key SERVICE MAINTENANCE key gt CONTINUOUS MEASUREMENT key CLOSED 10 SAMPLES key 6 When the READY screen is displayed press the following keys on each screen to display the 10 data X CV screen MENU key OTHER OPERATIONS key SERVICE MAINTENAN
20. Hex wrench thin type 4 Prepare the polymer microsphere suspensions in a sample tube and 10 blood samples of different healthy people within 12 hours after collection 5 Press the SETTINGS key on the MENU screen to display the SETTINGS screen 6 Press the SENSITIVITY THRESHOLD key on the SETTINGS screen to display the SENS amp THRESH screen SENS amp THRESH CBC Diff 04 AUG 02 14 53 SENSITIVITY amp THRESHOLD CBC SENS amp REPOL DIFF GAIN 7 Press the DIFF GAIN key to display the DIFF GAIN ROUGH screen uA 04 AUG 02 14 53 SCATTERGRAM HISTOGRAMS FS FL 50 5 FL PARTICLE COUNT cv 2935 IDEALPEAK 50 80 IDEALPEAK START t START START t t t CURRENT GAIN 80 CURRENT GAIN 71 CURRENT GAIN 35 TARGET GAIN TARGET GAIN TARGET GAIN 64 CLEAN FLOW Press manual mode 1 key The ideal peak and current gain values are displayed on the screen 6 2 Service Manual MEK 8222 6 ADJUSTMENT 8 Press the Manual mode key on the front panel to enter manual mode put SN the sampling nozzle into the bottom of the sample tube containing the polymer microsphere suspensions so that the tip of the sampling nozzle touches the bottom of the sample tube and press the Manual count switch The polymer microsphere suspensions is aspirated and measurement starts The result appears on the screen When the CV of FS
21. The instrument generates the following alarms as reagent alarms when the 4 channel liquid detection sensors in the Inlet Outlet unit cannot detect the presence of diluent detergent and hemolysing reagents NO DILUENT NO DETERGENT NO LYSING REAGENT 1 NO DILUENT Possible Causes Countermeasure When the diluent cannot be aspirated through the ISOTONACe3 diluent inlet Damage in the diluent aspiration fluid path or loose tube joint Check the fluid path Faulty mixing chamber Replace the mixing chamber The tube is disconnected or the tube has a leak Completely connect the tube between the instrument ISOTONACe3 container replace the tube with a new one The pump tube for MP 520V has a deterioration or leak Replace the pump tube with a new one When the diluent can be aspirated through Air bubbles stay on the liquid detector sensor Press the Clean key on the front panel ISOTONACe3 diluent Wrong setting of the sensor voltage Adjust the VR on the Liquid inlet Sensor board Faulty liquid detection sensor Replace the Liquid Sensor board 2 NO DETERGENT Possible Causes Countermeasure When the detergent Damage in the detergent aspiration fluid Check the fluid path cannot be aspirated path or loose tube joint through the CLEANAC Or CLEANACe3 detergent inlet Faulty mixing chamber Replace the mixing chamber
22. iii Explanations of the Symbols in this Manual and Instrument iv On E On screen and recorded data 20200220 40 V Ds c 1C 1 DUCTION mm 1 1 Sevice POM CY MM 1 2 5 c 1 3 Measured Parameters Ranges Reproducibility to Specimen from Venous 1 3 Detection Method ttr etm bb edna eue Pa 1 3 Standardization Analysis 1 3 Interference SUBSTANCES ro as E FEE deed 1 4 Dilin RAO isc m 1 6 COLIN MME e een 1 6 bin adele 1 6 Data 1 6 Environmental Conditions siisii ikainiai dinaan iiai 1 6 Power RD xad dun 1 6 Dimensions and 1 7 Electromagnetic Compatibility 1 7 ru LET 1 7 Panel Desci 1 8 Front PANG
23. 3 Disconnect the 2 cables from the table unit and remove the table unit from the instrument CAUTION in Assembling the Instrument Put the table unit back to the original position and move the unit to the left as much as possible Fasten the unit to the chassis with the black screws 4 8 Service Manual MEK 8222 4 DISASSEMBLY AND ASSEMBLY Removing the MO 820V Laser Optical Unit Service Manual MEK 8222 The laser optical unit consists of the following MO LD DRIVER board MO preamp boards Flow cell unit Laser diode 1 Remove the 2 nuts and 2 spacers from the laser optical unit as shown below Rear view 2 Disconnect the cable connected to the MASTER board at this board 3 Disconnect the 3 tubes from the flow cell unit Remove the tube joint No 4 from the MC 821V flow cytometry piping unit as shown below N 4 Remove the laser optical unit from the instrument 4 9 4 DISASSEMBLY AND ASSEMBLY Removing the MP 821V Sample Pump Unit The sample pump unit consists of the following Cylinder and piston 2 way valve XP 512V Stepping motor and lead screw Photo sensors 1 Remove the tube joint No 6 from the MC 821V flow cytometry piping unit 498009690 2 Remove the tube joint No 3 from the JQ 820V inlet outlet unit
24. One of the tubes is disconnected or the tube has a leak Completely connect the tube between the instrument and CLEANACe3 container replace the tube with a new one Completely connect the tube between the instrument and CLEANAC container or replace the tube with a new one The pump tube for MP 520V has a deterioration or leak Replace the pump tube with a new one When the detergent can be aspirated through the CLEANAC CLEANACe3 detergent inlet Air bubbles stay on the liquid detector sensor Press the Clean key on the front panel Wrong setting of the sensor voltage Adjust the VR on the Liquid Sensor board Faulty liquid detection sensor Replace the Liquid Sensor board Service Manual MEK 8222 3 NOLYSING REAGENT 2 TROUBLESHOOTING Possible Causes Countermeasure When the reagent hemolysing cannot be Faulty 3 way valve of the hemolysing pump Replace the 2 way valve aspirated through the Faulty cylinder block of the hemolysing Replace the cylinder block 5 inlet pump Faulty O ring of the hemolysing pump Replace the O ring Damage in the hemolysing reagent Check the fluid path aspiration fluid path or loose tube joint The tube is disconnected or the tube has Completely connect the tube a leak between the instrument and HEMOLYNACe5 container replace the tube with a new one
25. IDEAE PEAK START 80 e 7 102 61 IDEAL PEAK IDEAL PEAK START 101 START 114 147 52 CURRENT GAIN 80 CURRENT GAIN 71 CURRENT GAIN 35 TARGETGAIN 37 TARGET GAIN 50 TARGET GAIN 64 GAIN ADJUST Polymer microsphere suspensions measurement is complete Service Manual MEK 8222 1 GENERAL v Check the histogram PEAK FS approx 61 FS IDEAL PEAK FL PEAK approx 90 FL IDEAL PEAK Optimum Is PEAK OK NO Press AUTO GAIN key to change the current gain to the target gain Then measured PEAK changes to the IDEAL optimum PEAK C AUTO GAIN D y Press the OK key and the instrument starts CLEAN FLOWCELL procedure automatically C 2 Rough adjustment of FS size and FL complexity is complete v Check the background noise Measure a few empty sample tubes in the closed mode WBC 0 2 or less x10 uL Perform CLEAN RBC 0 05 or less x108 uL procedure HGB 0 1 or less g dL PLT 10 or less x10 uL TOC 100 or less count Is Data OK NO YES Service Manual MEK 8222 1 GENERAL 5 Perform fine adjustment of the gain with human blood samples Prepare 10 blood samples of different healty people within 12 hours after the blood collection v Measure the 10 human blood s
26. Open the window only when adjusting the gain Service Manual MEK 8222 1 GENERAL Refer to warnings and cautions in Connecting an External Instrument to the Hematology Analyzer in Section 2 2 4 5 6 Refer to warnings and cautions in Connecting the Power 7 Cord Grounding the Hematology Analyzer Section 2 No Name Description Connects to the optional WA 460V card printer WA 711V 1 5232 socket printer WA 820V printer or PC When is connected to this Socket histogram and scattergram data cannot be output 2 Bar code socket Connects to the optional ZK 820V handy bar code reader 3 USB socket Connects to a PC Histogram and scattergram data can be output 4 Main power swith Supplies the power to the hematology analyzer when it is turned on Under normal conditions keep this switch turned on Contains the time lag fuse To replace the fuse contact your Nihon Kohden distributor 6 AC SOURCE Connects the AC power cord to supply AC power to the AC source socket hematology analyzer 7 Equipotential ground Connects the ground lead to the equipotential ground terminal on terminal the wall for earth grounding Servic
27. When the hemolysing Wrong setting of the sensor voltage Adjust the VR on the Liquid reagent can be aspirated Sensor board through the Faulty liquid detection sensor Replace the Liquid Sensor board 5 inlet Service Manual MEK 8222 2 29 3 Doard Unit Description SUB EC 3 1 CBC MeasUtihg tp bac etna 3 1 Laser Optical Unit iiiter nr rr pen e Deb ausu pud os x Ue cad 3 1 Complex Pump 3 1 Sample Pump U nlt ee eei tene tat xx 3 2 Sheath PUMP U ofi RR REI I TT 3 2 Closed Sampler Unit sss eene nennen nnne nnne nnns enne nnns 3 2 3 2 Table Unit 3 2 e MM 3 2 MAS TER T 3 3 SLAVE CBC E 3 3 SLAVE 3 3 Service Manual MEK 8222 3C 1 3 BOARD UNIT DESCRIPTION The instrument consists of the following units and boards Sub Bath Unit The sub bath unit has two sub baths and motors which rotate the sub baths The two s
28. 0 291 pg MCHC 326 g dL Eosinophilia PLT 193 104 RDW 140 Comp lex ty Granalarity PCT 027 14 1H fL 18 3H Granular ity Therefore the room and fluid temperatures must be within the range of 15 to 30 C Service Manual MEK 8222 Sampled Whole Blood Handling Check Service Manual MEK 8222 2 TROUBLESHOOTING Blood Sample Handling for Counting To measure the white blood cells under stable conditions leave blood samples under the temperature range of 15 to 309 and count the blood samples within 8 hours after collecting them Blood Sample Handling for High Reliability To get highly reliable data do not count the blood samples which are left for more than 8 hours after the collection Counting the blood samples which are left for more than 8 hours may cause the 5 part ratios of the WBC to be out of the normal range even if the blood samples are collected from healthy persons Blood Sample from a Patient with Specific Conditions There is a possibility that WBC counts are inaccurately obtained from patients with specific conditions Especially the red blood cells from a patient who has hepatopathy liver disease certain special treatment or is neonatal have high resistance of their membranes against hemolysing reagent which causes insufficient hemolysing This will cause a lot of ghosts within the WBC count areas so that the ghosts can be counted as WBC It requires a measurement metho
29. 10 Aleak in the air tank Faulty regulator Faulty electromagnetic valve Tube joint is loose at the electromagnetic valve No fluid is in the air filter Remove the cause No display on the scattergram See Fig 12 Remove the cause The LASER switch on the left side of the instrument is set to OFF head cover if open faulty PREAMP board especially FS PREAMP board ul Faulty 5 DIFF board Wrong position of the MF 820V Adjust the flow cell position Refer to Section 6 Adjusting the Flow Cell Position 11 AUG 99 16 58 1832 g E 1 4L 18 uL 3 181 108 01 10 2L g dL 38 4 5 95 6 32 1 Pg 3 C 33 6 g dL 5 126 10 uL Comp lexi ty Granular ity 13 7 x 8 80 7 2 fL 20 0H i Granularity 109 2200 Fig 7A 2 22 Service Manual MEK 8222 Service Manual MEK 8222 11 AUG 99 17 20 N V Granularity 7 01 13 54 POOR HEMCLYZAT IO 11 10 99 Ix LY 25 9 98 Fig 7B Comp lexi ty 4 iff Granularity 7 1 DNO SI ZEOUER 2001 1 1931 Granular tty 7 FEB 1 13 27 Fig Comp lexi ty Granular ity W7 FEB 01 43 5 34 8 5 1 17 2H 8 2 4 lar tty 100 200 Fig 9 gt IDNO 2 TROUBLESHOOTING RBC HGB HCT CU CH PLT PDW 37 9
30. 3 Remove the 2 black screws which secure the sample pump unit to the chassis Slowly pull down the top of this unit and pull it upward until you can disconnect the 2 cables connected to the DRIVERI board at this board Rear view 4 Disconnect the 2 cables from the sample pump unit and completely remove it from the instrument 4 10 Service Manual MEK 8222 4 DISASSEMBLY AND ASSEMBLY Removing the MP 822V Sheath Pump Unit Service Manual MEK 8222 The sheath pump unit consists of the following Diaphragm pump 2 way valve XP 512V Air tank Regulator Pressure sensor and SHEATH CONTROL board Sheath tank and LIQUID LEVEL SENSOR board 1 Remove the 3 tube joints from the sheath pump unit 2 Disconnect the cable connected to the MASTER board at the sheath pump unit 3 Remove the 2 black screws which secure the sheath pump unit to the chassis Remove the unit from the instrument 4 DISASSEMBLY AND ASSEMBLY Removing the MC 821V Flow Cytometry Piping Unit 1 Remove the 6 tube joints No 4 to No 9 from the flow cytometry piping unit as shown below Disconnect the 3 tubes from the laser optical unit if connected A 499009990 2 Remove the tube joint No 1 from the JQ 820V inlet outlet unit 3 Disconnect the following cable from the DRIVERI board 4 12 Cable connected to the pump unit Cable connecte
31. 5 part diff uses the same For example method as the manual calibration procedure of the CBC but it must be done with human LY previous calibration coefficient 1000 blood samples LY mean other method s value 35 8 LY mean measured value 32 6 New calibration coeffitient LY new calibration coefficient 1000 x 35 8 32 6 Previous calibration coefficient 1098 x Mean other method s value Mean measured value 1 20 Service Manual MEK 8222 1 GENERAL How to Upgrade the Software Service Manual MEK 8222 Right side cover Instruments with a serial number between 00001 and 000xx require the following ROM replacement before the software upgrade Therefore check the serial number on the rear panel of the instrument If the serial number is not in the above mentioned serial numbers go to Upgrading the Software with Memory Card Replacing the ROM Turn off the main power switch on the rear panel of the instrument and unplug the AC power cord CAUTION Before removing any parts from the instrument wait 10 minutes after turning off the instrument and disconnecting the power cord from the AC outlet 2 Remove the screws which secure the side covers and top cover to the instrument chassis Remove the covers from the instrument 3 Left side cover Unplug the power cord 1 21 1 GENERAL 3 Remove the screws which secure the rear cover to the instrument chassis Slowly
32. 820V CBC Measuring Unit UT 7143 PRE AMP Board UT 7180 CBC Measuring Board Set UT 7181 HGB AMP Board UT 7182 HGB LED Board UT 7183 MANOMETER PTR Board UT 7184 MANOMETER LED Board 1 XP 512V 2 way Valve XP 513V 3 way Valve MC 821V Flow Cytometry Piping Unit UT 7152 DRIVER1 Board XP 512V 2 way Valve XP 513V 3 way Valve Service Manual MEK 8222 Service Manual MEK 8222 1 GENERAL MO 820V Laser Optical Unit MF 820V Flow Cell Unit UT 7172 MO Preamp Board Set UT 7173 MO FS PREAMP Board UT 7174 MO FL PREAMP Board UT 7175 MO SD PREAMP Board UT 7176 MO LD DRIVER Board MP 520V Pump Unit MP 820V Complex Pump Unit UT 7177 MD MP Connection Board Set UT 7186 MD CONNECTION Board UT 7187 MP CONNECTION Board XP 503V 3 way Valve XP 513V 3 way Valve MP 821V Sample Pump Unit XP 512V 2 way Valve MP 822V Sheath Pump Unit UT 7158 SHEATH CONTROL Board UT 7171 LIQUID LEVEL SENSOR Board XP 512V 2 way Valve MS 820V Closed Sampler Unit UT 7154 DRIVERS Board UT 7185 5 SENSOR Board XP 513V 3 way Valve MS 821V Open Sampler Unit UT 7065 SENSOR Board 1 GENERA
33. 85 V 2 14 V DETERGENT WBCLOW 046V 0 26 V HEMOLYNAC 3 RBC UP 0 91 V HEMOLYNAC 5 RBCLOW 0 23 CALIBRATION SWITCH SHEATH DILUENT MP 820V LIQUID TANK CBC ELECTRODE MC 820V SHEATH TANK WBC WBC 17 9 V RBC SHEATH PUMP RBC 17 9 V 1st PRESSURE 155 kPa 2nd PRESSURE 101 kPa WARMER TEMP 29 C INTERNAL TEMP 33 C DETERGENT Voltage detected by the detergent CLEANAC and CLEANAC3 detection sensor in the JQ 823V 4 channel liquid sensor unit HEMOLYNAC 3 Voltage detected by the hemolysing reagent HEMOLYNAC 3 detection sensor in the 4 channel liquid sensor unit HEMOLYNAC 5 Voltage detected by the hemolysing reagent HEMOLYNAC 5 detection sensor in the 4 channel liquid sensor unit SHEATH DILUENT Voltage detected by the diluent ISOTONAC 3 detection sensor in the 4 channel liquid sensor unit Liquid Normal Output Voltage Present 1 5 V or less Absent 3 5 V or more Service Manual MEK 8222 6 13 6 ADJUSTMENT LIQUID TANK Voltage detected by the diluent ISOTONAC 3 detection sensor on the UT 7171 LIQUID LEVEL SENSOR board in the JQ 822V center piping unit 6 SHEATH TANK Voltage detected by the diluent ISOTONAC 3 detection sensor on the UT 7171 LIQUID LEVEL SENSOR board in the P 822V sheath pump unit MP 822V Liquid Normal Output Voltage Present 3 5 V or more Absent 1 5 V or less 6 14 Service Manual MEK 8222 Service M
34. Left UT 7156 Back and Forth SLAVE CBC board 50 2 H8S m dNp235 UT 7147 CPU 71852 52 PHD10 SENSORS board vie T 6 102 CN203 UT 7086 AMP6P Posiion sensor POS 227 cnoos Position sensor CODEO je 10 014 CNoo2 br Position sensor CODE1 4 CN204 Position CODE2 CN204 HIF64P VH6P sees PHD12 HIF 16P CN203 ll CNA233 iss PHD24P 108 64 CNA013 229 TABLE SENSOR board 7157 HIF 64P VH6P CN101 00 2222222223 reader ZK 822M DRIVER4 board Option 98 60020066 0 7155 CN202 CNA220 Sub bath unit MB 820V 219 PHD12P MFC10P CNP222 3 2 zt 012 measuring unit MC 820V CN005 22 AMP board 07 7143 2 VH8P AMP10P ee CN205 CNO003 CN102 CN004 VH6P HIFSOP VH2P Position sensor z 8 CN208 CNA106 jj PHD18P CNOO1 Ei CNO002 VH6P HIF50P VH 2P MFC16p ovo CNP225 201 2 27202 em MFC16P Left side piping unit JQ 821V DRIVER2 board CNA239 NIA UT 71 53 ae sma Pump unit MP 520V Pump unit MPS20V WBC Position sensor CNA237 94204 05208 CNA236 1 GENERAL 1 27 1 GENERAL Hydraulic System Diagram gt cncencdsseeccuceneemxcenp a aaa aan aaa e
35. MEK 8222 2 TROUBLESHOOTING The following two cases require gain adjustment Refer to Section 6 Adjusting FS FL SD Gain and FS Threshold 1 Excessive high gain OXFEB 99 13 15 O2 FEB 99 000000000004 7 I0 uL WBC 45 103 uL NE 498 23 496 10 uL LY 35 9 1 6 151 g dL MO 53 02 HCT 461 7 EO 84H 04 MCV 929 fL BA 06 00 304 pg MCHC 328 g dL m PLT 214 109 RDW 11 5 PCT 033 MPV 158 fL PDW 213H Comp lexTty Granular ity RB B PLT 5 5 Granular ity 180 200 27197 20 2 Excessive low gain 02 99 13 15 02 99 IDNO 000000000004 4 I0 yuL WBC 47 10 uL NE 50 8 24 504 109 LY 347 L6 152 g dL 5 91 5 983 03 470 i EO 86H 04 933 2 2 00 00 302 MCHC 323 g dL 1 PLT 214 10 Comp lexity Granularity RDW 117 PCT 034 MPV 15 9H fL 5 RB 5 PLT PDW 2L3H s 5 2 6 Granularity Too 200 _ Service Manual MEK 8222 2 5 2 TROUBLESHOOTING The following two cases require threshold gain adjustment Refer to Section 6 Adjusting FS FL SD Gain and FS Threshold 1 Excessive high threshold gain O2 FEB 99 13 15 02 99 IDNO 000000000004 0050 WBC 47 103 uL NE 536 25 500 10 Ly 312 15 149 g dL MO 91H 04 HCT 465 EO 21 01 930 fL
36. Measuring Unitarian ERE IURE REI 3 1 Laser Optical 2 22 0 aaia ea re Enh nennen innen nennen nennen nnn nnn 3 1 ere elatum 3 1 Sample PUMP cr EU Lie 3 2 Shieath 3 2 Closed Sampler Unit eine eti beige dert teta ec HERR CRUS 3 2 Open Sampler Units c 3 2 TADO UN mI 3 2 Display 3 2 MASTER Board EMEN 3 3 SLAVE CBC Boardi S 3 3 5 M 3 3 Service Manual MEK 8222 5 Section 4 Disassembly and Assembly 4C 1 Removing the PV 820V Display Unit eiecti 4 1 Removing the MC 820V CBC Measuring Unit essssseseeeeneeennne 4 2 Removing the MB 820V Sub Bath 4 4 Removing the MP 820V Complex Pump Unit 4 5 Removing the MS 820V Closed Sampler Unit 2 4 6 Removing the MS 821V Open Sampler Unit 4 7 Removing the MS 822V Table Unit 0 002400440 000 nnne nnne nnn 4 8 Removing the MO 820V Laser Optical Unit 4 9 Removing the MP
37. SHEATH DILUENT 0 20 V LIQUID TANK 4 72 V SHEATH TANK 415 SHEATH PUMP 1st PRESSURE 2nd PRESSURE 155 kPa 101 kPa WARMER TEMP INTERNAL TEMP 29 C 33 C Diluent or Sample Normal Output Voltage Present 1 5 V or less Absent 3 5 V or more RBC manometer WBC manometer Service Manual MEK 8222 6 ADJUSTMENT WBC manometer upper sensor adjustment e Adjust the sensor output control VR1 until the WBC UP value on the screen shows 1 5 V or less when there is liquid at the sensor position level in the manometer e Adjust the sensor output control 1 until the WBC UP value on the screen shows 3 5 V or more when there is no liquid at the sensor position level in the manometer WBC manometer lower sensor adjustment e Adjust the sensor output control VR2 until the WBC LOW value on the screen shows 1 5 V or less when there is liquid at the sensor position level in the manometer e Adjust the sensor output control VR2 until the WBC LOW value on the screen shows 3 5 V or more when there is no liquid at the sensor position level in the manometer RBC manometer upper sensor adjustment e Adjust the sensor output control VR3 until the RBC UP value on the screen shows 1 5 V or less when there is liquid at the sensor position level in the manometer e Adjust the sensor output control VR3 until the RBC UP value on the screen shows 3 5 V or more when there is no liquid at the sensor
38. e Capillary blood Sample volume WBC HGB RBC PLT Counting Time 55 uL 200 1 40 000 1 10 uL 20 uL 1200 1 600 1 240 000 120 000 1 Approximately 45 s sample 80 samples hr Display Display Resolution Screen size Display contents Data Storage 8 4 inch TFT type color LCD with touch screen keys 800 x 600 dots approx 170 4 x 127 8 mm Numerical data scattergrams histograms measuring conditions alarm message and other messages touch screen keys Numerical data for all counted parameters for up to 400 samples and histograms and scattergrams for up to 60 samples Environmental Conditions Storage temperature 20 to 60 Operating temperature 15 to 30 C Storage humidity 10 to 95 Non condensing Operating humidity 30 to 85 Non condensing Storage atmospheric pressure 70 to 106 kPa Operating atmospheric pressure 70 to 106 kPa Power Requirements Power requirements MEK 8222J 110 117 or 127 V 10 AC 50 60 Hz MEK 8222K 220 230 or 240 V 10 AC 50 60 Hz Power consumption 360 VA 1 6 Service Manual MEK 8222 Dimensions and Weight Dimensions 613 W x 550 D x 583 H mm Net weight approx 55 kg Electromagnetic Compatibility IEC 61326 1 1997 Amendment 1 1998 Amendment 2 2000 EN 61326 1 1997 Amendment 1 1998 CISPR11 1997 Group 1 Class EN 55011 1998 Amendment 1 1999 Group 1 Class B Safety Safety standards IEC 61010 1 2nd Edition 2001 EN 61010 1 199
39. no diluent in the SHEATH DILUENT sensor block adjust the SHEATH DILUENT sensor output control 4 until the value indicates 3 5 V or more b When the SHEATH DILUENT sensor block is filled with diluent adjust the SHEATH DILUENT sensor output control 4 until the value indicates 1 5 V or less SENSOR MONITOR CBC Diff SHEATH DILUENT sensor CBC MANOMETER HGB SENSOR LIQUID SENSOR WBC UP 0 85 V 244 V DETERGENT WBCLOW 046V 0 26 V HEMOLYNAC 3 RBC UP 0 91 V HEMOLYNAC 5 RBCLOW 0 23 CALIBRATION SWITCH SHEATH DILUENT 0 20 V MP 820V LIQUID TANK 472 CBC ELECTRODE MC 820V SHEATH TANK 415 V WBC WBC 17 9 V RBC SHEATH PUMP 9 V BER 1st PRESSURE 155 kPa 2nd PRESSURE 101 kPa WARMER TEMP 29 C INTERNAL TEMP 33 C SHEATH DILUENT sensor block Output control 4 6 18 Service Manual MEK 8222 Pressure Sensor Service Manual MEK 8222 6 ADJUSTMENT The pressure values detected by the pressure sensors in the sheath pump unit are displayed EE CBG Dif 07 JUL 02 14 53 CBC MANOMETER HGB SENSOR LIQUID SENSOR WBC UP 0 85 V 2 14 V DETERGENT 0 16 V WBCLOW 046 0 26 HEMOLYNAC 3 0 23 V RBC UP 0 91 HEMOLYNAC 5 0 21 V RBC LOW 0 23 V CALIBRATION SWITCH SHEATH DILUENT 0 20 V MP 820V LIQUID TANK 472V CBC ELECTRODE MC 820V SHEATH TANK 415 V WBC WBC 17 9 V RBC SHEATH PUMP nee 1st PRESSURE 155 kPa 2nd PRESSURE 101 kPa WARMER TEMP 29 C INTERNAL TEMP 33 C CD 1st PRESSURE Pressure detected
40. not completely lyse and these non lysed RBC may be detected as WBC and cause increase in WBC count Multiple myeloma The precipitation of proteins in multiple myeloma patients may increase the WBC count Leukemia WBC is fragile in leukemia patient and WBC may be destroyed during measurement These WBC fragments may also interfere with WBC differential measurement Chemotherapy Cytotoxic and immunosuppressive drugs cause low WBC count Cryoglobulins Cryoglobulin may be increased in patients who are or have myeloma cancer leukemia macroglobulinemia lymphoproliferative disorders metastatic tumors autoimmune disorders infections anerurism pregnant thromboembolic phenomena diabetes etc which cause increase in WBC RBC or PLT counts and HGB concentration In such cases warm the blood sample to 37 C in a water bath for 30 minutes and measure the sample immediately RBC Leukemia An increase in WBC in leukemia patient causes increase in RBC Agglutinated RBC Agglutinated RBC may decrease RBC count This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Cold agglutinins IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and increase MCV HGB Turbidity of the blood sample Any physiologic and or therapeutic factors may increase HGB In such a case determine the cause of turbidity and follow the appropriate method below 1 Increased WBC An extreme increase
41. position level in the manometer RBC manometer lower sensor adjustment e Adjust the sensor output control VR4 until the RBC LOW value on the screen shows 1 5 V or less when there is liquid at the sensor position level in the manometer e Adjust the sensor output control VR4 until the RBC LOW value on the screen shows 3 5 V or more when there is no liquid at the sensor position level in the manometer Service Manual MEK 8222 6 9 6 ADJUSTMENT CBC Electrode 6 10 The differential voltages between the two electrodes are displayed The normal range of the differential voltage of these electrodes is 18 3 V 0 3 V SENSOR MONITOR CBC MANOMETER WBC UP WBC LOW RBC UP RBC LOW CBC ELECTRODE zi HGB SENSOR ON 2 14 V OFF 0 26 V CALIBRATION SWITCH MP 820V MC 820V WBC RBC 07 JUL 02 14 53 LIQUID SENSOR DETERGENT 0 16 V HEMOLYNAC 3 0 23 V HEMOLYNAC 5 0 21 V SHEATH DILUENT 0 20 V LIQUID TANK 4 72 V SHEATH TANK 435 V SHEATH PUMP 1st PRESSURE 2nd PRESSURE WBC electrode Service Manual MEK 8222 6 ADJUSTMENT HGB Sensor HGB sensor output voltage is displayed ON Output voltage from the sensor in the HGB measuring block of the CBC measuring unit when the LED is lit The HGB SENSOR ON voltage must be in the range of 3 0 V 0 3 V OFF Output voltage from the sensor in the HGB measuring block of the CBC measuring unit when the LED is not lit HGB measuring block Remove th
42. the 7 um polymer microsphere suspensions FS CV must be 7 or less Is CV OK YES FL CV must be 7 or less Adjust the flow cell position Remove the flow cell unit window cover and loosen the screw fastening the flow cell adjustment screw Y Turn the flow cell adjustment screw about 2 or 3 Perform CLEAN FLOW CELL procedure C MENU C OTHER OPERATIONS C CLEAN ERE CELLS D NO small oval shape as shown below is result is optimum the CV values of FS and FL are below 7 The histogram is narrow and scattergram is If the result is not optimum the CV values of FS and FL are over 7 The histogram is wide and scattergram is large as shown below DIFF GAIN ROUGH SCATTERGRAM PARTICLE COUNT 2935 HISTOGRAMS FEET t Ed E 57 IDEAL PEAK 61 START Fd ENDt CURRENT GAIN 75 TARGET GAIN 64 pa 4 ff 4 5 19 IDEALPEAK 90 ADU 94 19 CURRENT GAIN 79 TARGETGAIN 65 CBC Diff 07 JUL 02 14 53 80 949 PEAK 15 IDEAL START T o 54 CURRENT GAIN 103 TARGETGAIN 64 Polymer microsphere suspensions measurement is complete DIFF GAIN ROUGH SCATTERGRAM FL PARTICLE COUNT 2935 HISTOGRAMS CBC Diff 07 JUL 02 14 53 pm
43. the screen To install the LANGUAGE software press the LANGUAGE local language Vxx xx xxxx key on the screen To install the ANALYSYS PROGRAM software only press the ANALYSYS PROGRAM Vxx xx xxxx key on the screen eG 8 DOWNLOAD MEK 8222 PROGRAM V01 02 MEMORY CARD CHECK SUM MASTER BD 4649 C590 DOWNLOAD A2BA ALL PROGRAM 2 11 ANALYSIS PROGRAM 01 01 0130 DOWNLOAD SEPARATERY MASTER BD LANGUAGE ENGLISH V01 01 4649 V01 01 2F11 ANALYSIS PROGRAM 01 01 0130 SLAVE CBC BD V01 01 C53D SLAVE MS BD V01 01 A2BA 1 25 1 GENERAL After the software is installed the following screen appears DOWNLOAD MEK 8222 PROGRAM V01 02 MEMORY CARD M CHECK 5 MEK 8222 SYSTEM SUM MASTER BD 4649 4649 SLAVE CBC BD C53D C53D SLAVE MS BD A2BA A2BA LANGUAGE ENGLISH 2 11 2F11 ANALYSIS PROGRAM 0130 0130 Download complete Please press OK key after remove memory card 7 Press the OK key on the screen The instrument starts automatic priming 8 Press the card eject key and remove the memory card from the instrument 9 Reassemble the instrument completely 1 26 Service Manual MEK 8222 Connection and Wiring Diagram Impact Printer WA 711V Option or locally avialable printer LQ 300 Data Management Program for PC Qi 820V Option Handy Barcode Reader Unit ZY 820V Option 1 300 LX 300
44. to the ACTUAL PEAK LIST 7 Auto gain adjustment Press the AUTO GAIN key to change the current gain to the target gain Manual gain adjustment You can also change the gain by using the numeric keys on the screen Press the MANUAL GAIN key use the arrow keys to move the cursor to the desired item and enter the number in the EDIT GAIN column Service Manual MEK 8222 6 5 6 ADJUSTMENT To adjust size vertical axis of the scattergram adjust FS GAIN To adjust complexity horizontal axis adjust FL GAIN To adjust granularity horizontal axis adjust SD GAIN 8 Press the REFRESH key to draw scattergrams with the gain entered in the EDIT GAIN boxes 9 Press the ADD TO LIST key to register the data in the ACTUAL PEAK LIST 10 When the gain is adjusted go to the READY screen and measure human blood sample to check that the scattergrams are optimum and no flags are displayed 6 6 Service Manual MEK 8222 6 ADJUSTMENT Adjusting Sensors SENSOR MONITOR screen The SENSOR MONITOR screen displays the real time outputs of the sensors in the instrument The displayed output values tell you whether the sensor is functioning correctly out of adjustment or faulty How to call up the SENSOR MONITOR screen 1 READY screen 2 Press the MENU touch screen key MENU screen appears 3 Press the OTHER OPERATIONS touch screen key OTHER OPERATIONS screen appears 4 Press the SERVICE MAINTENANCE touch scre
45. which results in low MPV Very small fragments Very small RBC RBC fragments and WBC fragments may interfere with MPV measurement Agglutinated RBC PLT may be trapped in the agglutinated RBC resulting erroneous MPV This can be checked by abnormal MCH and MCHC values and examination of the stained blood film Chemotherapy Cytotoxic and immunosuppressive drugs may affect MPV In such a case measure manually NOTE Blood samples collected in EDTA does not maintain stable MPV because platelets swell depending on the interval after collection and storage temperature Service Manual MEK 8222 1 5 1 GENERAL WBC 5 part differential parameters derived from the WBC count therefore the limitations for WBC also affect these parameters LY and LY Erythroblasts certain parasites and RBC that are resistant to lysis may interfere with an accurate LY count MO and Large lymphocytes atypical lymphocytes blasts and excessive number of basophils may interfere with an accurate MO count NE and Excessive eosinophils metamyelocytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate NE count and NE EO and EO Abnormal granules may interfere with an accurate EO count BA and Immature cell metamyelocytes myelocytes promyelocytes blasts and plasma cells may interfere with an accurate BA count and Dilution Ratio e Venous blood Sample volume WBC HGB RBC PLT
46. 3 Amendment 2 1995 Laser IEC 60825 1 1993 Amendment 1 1997 EN 60825 1 1994 Amendment 11 1996 According to the type of protection against electrical shock CLASS I EQUIPMENT According to the degree of protection against harmful ingress of water IPXO Ordinary EQUIPMENT 1 GENERAL According to the degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH AIR OR WITH OXYGEN OR NITROUS OXIDE EQUIPMENT not suitable for use in the presence of FLAMMABLE ANAESTHETIC MIXTURE WITH AIR OR WITH OXYGEN OR NITROUS OXIDE According to the mode of operation CONTINUOUS OPERATION EQUIPMENT types classification Indoor stationary EQUIPMENT Installation Category II EQUIPMENT Pollution Degree 2 EQUIPMENT Requirements for marking of in vitro diagnostic instruments EN1658 1996 Service Manual MEK 8222 1 GENERAL Panel Descriptions Front Panel 4 5 CAUTION Do not put your hand in the rack table during measurement No Name Description 1 Main power lamp Lights when the main power switch on the rear panel is turned on Turns the hematology analyzer power on or off when the main power switch on the rear panel 2 Power key 15 turned on When the power is turned on priming and self check are automatical
47. 3 MS 820V Na RINSE SAMPLE CUP Jp DEG 27 150 12 JQ e22V L2 CLEANAC CLEANAC 3 T1 HEMOLYNACe3 HEMOLYNACe5 gt ISOTONAC e3 gt WASTE
48. 4321 Enter The status bar on the screen is displayed in orange Service Manual MEK 8222 1 GENERAL PRIME ON INSTALLATION is special procedure after storing and transporting the instrument OPERATORS amp PASSWORDS 30 NOV 01 14 53 CURRENT OPERATOR MEK F LAB TEQ OPERATOR TYPE LAB TECHNICIAN PASSWORD OPERATOR TYPE OF OPERATOR MANUFACTURER e LAB TECHNICIAN O 22 54418 OTHER CANCEL SELECT ADD OPERATOR OPERATOR MEK 8222 has 3 default operator types 1 MANUFACTURER password 4321 for servicing Allows you to enter any screen and change any settings 2 LAB TECHNICIAN password 8222 Allows you to enter any screen and change settings except for the SERVICE MAINTENANCE screen 3 OTHER password 0000 Allows you to only perform the measurement and view the data and settings Operator name MEK F Type of Operator LAB TECHNICIAN is selected as the default setting 1 15 1 GENERAL v suspensions Check the laser optical unit and roughly adjust the gain with 7 ium polymer microsphere Display the DIFF GAIN ROUGH screen C MENU 4 v SETTINGS y SENSITIVITY amp THRESHOLD v C DIFF GAIN Measure
49. 821V Sample Pump Unit 4 10 Removing the MP 822V Sheath Pump Unit 4 11 Removing the MC 821V Flow Cytometry Piping 4 12 Removing the MP 520V Pump 4 14 Section 5 Socket Pin Assignment eese eese esses eene nena nnn 5C 1 Socket Pin ASSIGHIMEME cba teal iet 5 1 AS23826 SOCKCE 5 1 ZK B20V SOCKET naana iaaa 5 1 idl eee ele A 5 1 cong Adjustment E 6C 1 Adjusting the Flow Cell 6 1 Adjusting the FS FL SD Gain and FS 6 4 Adjusting S6HsO s stone tte T ets te ebd a ead dues 6 7 SENSOR MONITOR screen sssssssssseseeeeeneeeneeeen nnne 6 7 How to call up the SENSOR MONITOR screen 6 7 6 8 WBC manometer upper sensor adjustment 6 9 WBC manometer lower sensor 0400 6 9 RBC manometer upper sensor 6 9 manometer lower sensor adjustment 6 9 CBC
50. ATTERGRAM key to draw scattergrams of the sequence number displayed for NEW SAMPLE DIFF GAIN FINE CBC Dift 04 AUG 02 14 53 SCATTERGRAM ACTUALPEAK 163 80 51 IDEALPEAK 185 53 25 CURRENT SAMPLE SEG 0001100 ADD TO LIST D NEW SAMPLE SEQH 0001123 qe L6 ENABLE SEQ 0001100 0001123 SCATTERGRAM ooo rn FSGAIN 80 93 FLGAIN 65 45 SDGAIN 64 21 FSTHR 5 5 o eo 6 When the scattergrams are oval in shape and not touching the division line press the ADD TO LIST key to register the data in the ACTUAL PEAK LIST It does not matter if the scattergrams are not within their allotted area Check Points Check that the WBC distribution 1 is in the square ABED and does not cover the lines AB BE ED and DA E Check that the WBC distribution 2 is in the square and does not cover the lines BC CH HG and GB Check that the WBC distribution 3 is in the square DEGF and does not cover the lines DE EG GF and FD Check that the WBC distribution 4 is in the square and does not cover the lines IJ JM ML and LI Check that the WBC distribution 5 is in the triangle OPR and does not cover the lines OP PR and RO M Granularity N When the scattergrams are not optimum do not register the data in the gain list Draw scattergrams of all samples you measured in step 14 You must have at least one data which has been added
51. C 820V CBC Measuring Unit The CBC measuring unit consists of the following Two apertures 80 um in diameter for RBC 100 um in diameter for WBC PRE AMP board Two manometers and MANOMETER boards for RBC PLT and WBC HGB measuring block HGB AMP board HGB LED board Two measurement baths Electromagnetic solenoid valves Air chamber Manual measurement switch Remove the 7 tube joints Index No 15 16 17 18 20 21 22 from the CBC measuring unit 19 410 ony 2 Remove the sample cup from the CBC measuring unit by pressing the hook of the sample cup and turning the sample cup down 4 2 Service Manual MEK 8222 Service Manual MEK 8222 4 DISASSEMBLY AND ASSEMBLY Remove the 2 black screws which secure the CBC measuring unit to the chassis as shown below Slowly remove the CBC measuring unit from the instrument while removing the 2 sub baths from the shafts Front view Disconnect the 2 cables connected to the SLAVE CBC board at the CBC measuring unit Disconnect the 2 cables connected to the DRIVER2 board at this board Completely remove the CBC measuring unit from the instrument 4 3 4 DISASSEMBLY AND ASSEMBLY Removing the MB 820V Sub Bath Unit 1 Remove the 2 black screws which secure the sub bath unit to the chassis 2 Disconnect the 2 cables connected to the DRIVER4 bo
52. CE key 10 data X CV key 7 Press the PRINT key on the screen to print the 10 data X CV table 8 Press the QUIT key on each screen until the MENU screen appears 9 Press the DATA EDITING key on the MENU screen to display the DATA EDITING screen 10 Delete the 10 sample data on tomorrow s date Refer to the MEK 8222J K operator s manual Section 6 DeletingData 2 8 Service Manual MEK 8222 CV Data Check Service Manual MEK 8222 2 TROUBLESHOOTING Since the following parameters show you the data reproducibility of the instrument check the printout data e CV of NE 90 5 or less at NE 50 e CV of LY 90 5 or less at LY 40 e CV of 12 or less at 5 CV of 20 or less at 4 e CV of BA 40 or less at BA 1 If the CV data of a parameter is higher than the above values it means poor reproducibility of the parameter The data reproducibility statistically depends on the number of cells If there are more of one type of cells the CV is lower for the more numerous type of cells Since MO EO and BA are much less than LY and NE the CV for 0 EO and BA is much higher than for LY and NE Especially BA is less than 1 in a healthy person s blood Therefore the CV of can reach up to 40 2 9 2 TROUBLESHOOTING Check Items Before Instrument Check Check Items before Instrument Check 2 10 Use the instrument and diluent under t
53. HOOTING The following example shows that the 5 parts of WBC are too widely scattered to group them 30 JUL 99 12 59 3 JUL 99 IDNO KOHDEN 2013 189 L 1 5 1 18 L RBC 5 1924 HGB HCT MCU MCH MCHC Granular ty RDW PCT MPU PDW Granular ity lt Fig 5 gt g L Possible Causes Countermeasure The blood sample is from a person who has a specific blood characteristic 1 The blood sample is from a person who has too many WBC to group normally See Fig 6 These causes do not result in incorrect data Wrong position of the flow cell unit If the blood cell which flows in the flow cell does not meet the center of 2 the laser beam the scattered light intensity varies according to the blood cell position in the flow so that each part of the WBC cannot be clearly grouped See Fig 5 Adjust the flow cell position optimally Refer to Section 6 Adjusting the Flow Cell Position Turbulent flow of the sheath diluent and diluted blood sample in the flow cell Adjust the flow cell position by counting 7 um polymer microsphere suspensions according to Section 6 Adjustment If one of the FS and FL coefficients of variation is still more than 7 0 after the flow cell position adjustment one of the following causes will make turbulent flow in the flow cell instrument is used where there is vibration T
54. L Not available MS 822V Table Unit UT 7086 SENSORS Board UT 7156 DRIVERS Board UT 7157 TABLE SENSOR Board PV 820V Display Unit UT 7178 Front Panel Board Set UT 7149 LCD CONTROL Board UT 7150 MANUAL KEY Board UT 7151 RACK KEY Board RK 820V Chassis SC 820VJ Power Supply Unit SC 820VK UT 7146 MASTER Board SLAVE CBC Board SLAVE MS Board Board Board INTERFACE Board nit WARMER CONTROL Board UT 7147 UT 7148 UT 7153 DRIVER2 UT 7155 DRIVER4 UT 7160 POWER Board UT 7161 ZY 820V Warmer U UT 7159 XP 513V 3 way Valve Service Manual MEK 8222 Installation Flow Chart Place the instrument in a suitable place Connect the power cord reagents and external equipment to the instrument Y Turn on the instrument Y Prime the instrument C MENU OTHER OPERATIONS v C PRIME ON INSTALLATION Y Change the OPERATOR to FACTORY to service the instrument Display the OPERATORS amp PASSWORDS screen a MENU C Senne gt Y OPERATORS amp PASSWORDS Y Select FACTORY FACTORY Y C SELECT OPERATOR y Enter the passwords
55. LIBRATION Press the exact center of the mark Last point 6 23
56. Manual MEK 8222 2 TROUBLESHOOTING UT FEB O1 14 41 QT FEB 01 IDNO NORMQL 0001 x 180 uL WBC 7 2 180 uL 6 2 4 9 5 17 105 uL 1 8 15 5 47 0 MCU 98 9 MCH 30 0 33 0 PLT 214 RDW 11 40 80 21 MPU 1 2 PDW 18 3 Fig 3 The edge of neutrophil distribution is in the MO amp BA area on the Size Complexity scattergram because of specific blood characteristics It is not caused by a wrong setting or malfunction of the instrument Service Manual MEK 8222 2 17 2 TROUBLESHOOTING e Scattergram 2 The following example shows that the 5 parts of WBC are not clearly separated because of too many ghosts Too many ghosts will decrease the WBC count and stay at the NE amp EO area so that the EO percentage may be excessively higher 02 FEB 99 13 15 02 FEB 99 IDNO 000000000004 96 10 uL WBC 48 107uL RBC 5 16 10 uL HGB 15 0 g dL HCT 46 0 891 fL 291 MCHC 326 g dL Eosinophilia PLT 193 103 uL RDW 140 Comp lexity Granularity en 4 PDW 18 3H Granularity Fig 4 Possible Causes Countermeasure Even if the blood sample is collected from a healthy Not required if the ghosts are scattered out of the person the scattergram may show that there are a lot of scattergrams ghosts because of specific blood characteristics There is a possibility t
57. STE 1 28 Service Manual MEK 8222 Jenue N coc8 MAW 6c lt MC 820V gt Index Model Number Remarks lt MP 820V gt 1 YK Index Model Number Remarks YK CO YK CO YK Tube with ground terminal 180 mm long CO CO YK CO YK BO YK DO YK YK SO CO NY D BY YK AD AC AD AC AD AC A RR EIS Tube with capacitor and ground terminal 240 mm long Model Number YK CO YK CO YK BD MC 821V Index Model Number Remarks YK DO DD AC AO DO 547953 7 5 m tube 1 2 3 4 5 6 7 8 9 0 1 2 3 4 3 6 7 8 9 547953 60 7 5 m tube AD Index Model Number Remarks 1 YK DO 305 2 YK CO 305 3 YK DO 270 4 YK CO 330 5 YK AC 400 6 YK BC 450 7 YK CD 170 Model Number Remarks YK 00003 Tube with grommet 615 mm long lt MP 822V gt Index Model Num
58. U Ooo ENABLE 5 0 0001100 0001123 SCATTERGRAM GAIN O IRR CURE ERES UEM FSGAIN 80 93 FLGAIN 65 45 co a re 5 s Coco CURRENT SAMPLE SEQ 0001100 AUTO GAIN Draw the scattergram with the changed GAIN 5 MANUAL Service Manual MEK 8222 1 GENERAL Measure the human blood samples once again and check the data Check the following points Scattergrams are oval in shape and are not touching the division line Flags do not appear especially Blasts Immature Granulocyte Left Perform the fine adujstment of the gain Shift Atypical Lymphocytes with human blood samples again 15 Data OK NO YES v Check the CBC calibration with the MEK 3DN Measure MEK 3DN in closed mode Data should be within the assay values Only CBC parameters Are all parameters OK CAL CLOSED CBC Diff 30 NOV 01 14 53 CLOSED CAL ID 0001 MEASURED CALIBRATION VALUE COEFFICIENT Rec Doo 7 Ces NO xol lo cp y Auto calibration procedure C MENU 7 v MPV 870 CALIBRATION C CLOSED AUTO CAL MODE Qu Measure the MEK 3DN in the closed mode CAL CLOSED 30 NOV 01 14 53
59. Up to 50 sample tubes can be accommodated 16 Rack table Holds the sample rack 1 8 Service Manual MEK 8222 Right Side Panel CAUTION Do not insert an object such a pencil into these holes Otherwise the liquid sensor unit may be damaged 1 GENERAL CAUTION Always carry the hematology analyzer by more than two people and by the left and right sides Never hold it by the front and rear Otherwise the front panel may come off and the hematology analyzer may be dropped causing injury to the operator and damage to the hematology analyzer No Name Description Turns the laser on or off with the laser key for WBC 5 part 1 Laser switch differential measurement ISO3 2 Inlets for the ISOTONAC 3 diluent Diluent inlets 3 Inlet for CLEANAC detergent Detergent inlet 4 Inlet for the CLEANAC 3 detergent Detergent inlet 5 HOM Inlet for HEMOLYNAC 3 hemolysing reagent Hemolysing reagent inlet 6 Inlet for the 5 hemolysing reagent Hemolysing reagent inlet 7 WASTE Outlets for waste such as used lyse detergent and aspirated Waste outlets samples 8 Flow cell unit window When two screws and the cover are removed you can see the cover flow cell unit
60. WBC 47 RBC HGB HCT MCV MCH MCHC PLT 238 RDW 1141 0 16 MPV 7 0 fL PDW 183H 103 uL 106 41 When the instrument works normally it displays a normal scattergram such as If a scattergram different from the above example is displayed there are a lot of possible causes as shown below e Scattergram 1 The following example shows that the 5 parts of WBC are grouped but they are not in the area allotted by the division lines Possible Causes Countermeasure Gain of the amplifier which amplifies the scattered light pulses is set incorrectly See Fig 1 Adjust the gain correctly Refer to Section 6 Adjusting FS FL SD Gain and FS Threshold The inside wall of the flow cell is dirty Albumin adhered to the inside wall of the flow cell can decrease the scattered light intensity See Fig 1 Press the Clean key to clean the wall inside the flow cell The blood sample collected from a person is left for more than 8 hours It can cause hemolyzed WBC See Fig 2 Use the blood sample within 8 hours after collecting the blood sample Even if the blood sample is collected from a healthy person the scattergram may show a pattern different from normal because of specific blood characteristics See Fig 3 Not required because there is no problem Laser beam intensity decreases without the above causes 1 and 2 The wrong setting of the laser beam focus or deterioration of t
61. amples in the closed mode Y Call up the DIFF GAIN FINE screen C MENU SETTINGS D SENSITIVITY THRESHOLD C DIFF GAIN C ADJUST GAIN FINE D v Load the scattergram and calculate the target gain Enter the measured sample s sequence number v LOAD NEW SCATTERG RAM v C ADD TO LIST e MO PEAK FS FL SD is displayed But the measured PEAK may be different from the IDEAL PEAK and the scattergram can touch the division line Press the AUTO GAIN key to change the current gain to the target gain Then the measured PEAK changes t the IDEAL PEAK Rough adjustment of FS size and FL complexity with 7um particle is already completed Adjustment of SD granularity is not yet completed DIFF GAIN FINE CBC Diff 07 JUL 02 14 53 SCATTERGRAM FL 50 50 0000000 ADD LIST NEW SAMPLE SEQ LOAD NEW ENABLE SEQ 0001700 0001123 SCATTERGRAM EDIT TARGET GAIN CURRENT SAMPLE SEQ CURRENT GAIN REFRESH Since the PEAK of Monocyte of human blood is the most stable parameter in WBC 5 part diff the optical fine adjustment is done with the MO PEAK DIFF GAIN FINE C CBC Diff 07 JUL 02 14 53 SCATTERGRAM ACTUALPEAK 163 80 51 IDEALPEAK 188 49 25 AoD To oo rcc Am U
62. and FL are below 796 go to step 10 DIFF GAIN CROUGH CBC Diff 04 AUG 02 14 53 n tf f t t PARTICLE COUNT P 10 7 2835 23 IDEAL PEAK IDEAL PEAK IDEAL PEAK START f START f 101 START f 2 ENDf ENDf 147 ENDf 52 CURRENT GAIN 80 CURRENT GAIN 71 CURRENT GAIN 35 TARGET GAIN 37 TARGET GAIN 50 TARGET GAIN 64 CLEAN FLOW SCATTERGRAM HISTOGRAMS FL microsphere suspensions measurement is complete When the result is not optimum press the CLEAN FLOW CELLS key to clean the flow cell unit After cleaning measure the polymer microsphere suspensions again When the result is still not optimum do the next step 9 Turn the flow cell adjustment screw about 2 or 3 and press the CLEAR MA key to redraw the histograms The polymer microsphere suspensions measurement lasts for about 25 about 2 to 3 seconds If adjusting the flow cell unit is not complete within this period measure polymer microsphere suspensions again If the histograms are narrower than before turn the flow cell adjustment screw in the same direction If the histograms are wider than before turn the flow cell adjustment screw in the opposite direction Repeat drawing histograms adjusting flow cell unit and redrawing histograms until you obtain the optimum result 10 Fasten the flow cell unit adjustment screw 11 Press t
63. anual MEK 8222 4 channel liquid sensor unit adjustment 1 Call up the SENSOR MONITOR screen 2 Adjust the DETERGENT sensor as follows a 6 ADJUSTMENT When there is no detergent in the detergent sensor block adjust the DETERGENT sensor output control 1 until the value indicates 3 5 V or more When the detergent sensor block is filled with detergent adjust the DETERGENT sensor output control 1 until the value indicates 1 5 V or less SENSOR MONITOR CBC MANOMETER WBC UP 0 85 V WBCLOW 046 RBC UP 0 91 V RBC LOW 0 23 V CBC ELECTRODE WBC 17 9 V RBC 17 9 V DETERGENT sensor block HGB SENSOR 244 V 0 26 V CALIBRATION SWITCH MP 820V MC 820V WBC RBC DETERGENT sensor LIQUID SENSOR DETERGENT HEMOLYNAC 3 HEMOLYNAC 5 SHEATH DILUENT LIQUID TANK SHEATH TANK SHEATH PUMP 1st PRESSURE 2nd PRESSURE WARMER TEMP INTERNAL TEMP 0 23 V 0 21V 0 20 V 472 V 4 15 V 155 kPa 101 kPa 29 C 33 C Output control 1 6 15 6 ADJUSTMENT 3 Adjust the HEMOLYNAC 3 sensor as follows a When there is no HEMOLYNAC 3 in the HEMOLYNAC 3 sensor block adjust the HEMOLYNAC 3 sensor output control 2 until the value indicates 3 5 V or more b When the detergent sensor block is filled with HEMOLYNAC 3 adjust the HEMOLYNAC 3 sensor output control 2 until the value indicates 1 5 V or less SENSOR MONITOR HEMOLYNAC 3 sensor CBC MANOMETER HGB SENSOR LIQUID SENSOR
64. ard at this board 3 Remove the sub bath unit from the instrument Front view 4 4 Service Manual MEK 8222 4 DISASSEMBLY AND ASSEMBLY Removing the MP 820V Complex Pump Unit The complex pump unit consists of the following Cylinder and piston 3 way valves XP 513V and XP 503V MP CONNECTION board MD CONNECTION board Stepping motors and ball screws 1 Disconnect the 2 cables connected to the DRIVER4 board at this board 2 Remove the 8 tube joints from the JQ 822V center piping unit ZY 820V warmer unit and MS 820V closed sampler unit 3 Remove the 2 black screws which secure the complex pump unit to the chassis 4 Remove the complex pump unit from the instrument Service Manual MEK 8222 PS4 x12 Front view 4 5 4 DISASSEMBLY AND ASSEMBLY Removing the MS 820V Closed Sampler Unit The closed sampler unit consists of the following Photo sensors Stepping motors and ball screws 3POS SENSOR board Rinse unit Mixing unit 1 Disconnect the 2 cables connected to the SLAVE MS board at the closed sampler unit Su ESA wA q at 55 524 E gd Ra 73 5 5 Front view 2 Remove the 5 tube joints from the closed sampler unit uw 3 Remove the 4 black screws which secure the closed sampler unit to the chassis 4 Remove the closed
65. ber Remarks 1 YK 130 2 BD 120 3 YK BO 065 4 YK CO 160 5 YK DO 150 6 YK BC 250 7 YK BC 325 8 YK CO 320 9 YK DO 110 10 YK DO 160 DO 210 12 559147 30 7 5 m tube 13 559147 55 7 5 m tube 14 559147 100 7 5 m tube 15 559147 230 7 5 m tube 16 534102 210 7 5 m tube 17 534102 230 7 5 m tube Note To use 7 5 m tube cut it in the optimal length 151 eqni TWHANAD 061 c668 MJIN lt IQ 820V gt Index Model Number Remarks 1 FO 130 2 YK AO 150 3 YK AO 120 4 YK FO 075 5 YK DF 110 6 YK CF 120 7 YK FO 160 8 YK FO 170 9 YK BF 140 0 YK BO 180 11 140 2 100 3 140 4 150 5 YK AO 160 6 YK AO 130 7 YK BO 230 8 YK CD 130 9 YK AC 120 20 YK AL 500 lt 10 821 Index Model Number Remarks YK CD YK YK AC DK BC DO DK NI D lt JQ 822V gt lt MEK 8222 gt Index Model Number Remarks Index Model Number Remarks AC AO AB BO AA BO AO BO AO DO CO x BD
66. blem 1 Scattergram and Histograms do not appear The TOC total optical count is 0 Possible Cause Countermeasure The LASER switch 15 set to off Remove the cause Failure of MP 822V No flow of the sheath fluid Replace the MP 822V with a new one Failure of MP 821V No flow of the sample Replace the MP 821V with a new one Wrong position of the MF 820V Adjust the flow cell position Problem 2 CV of FS and FL is above 7 0 Possible Cause Countermeasure Wrong position of the MF 820V Adjust the flow cell position Inside of the flow cell is dirty There are bubbles in the flow cell Perform the CLEAN FLOW CELL procedure 7 um polymer microsphere suspensions are wrong For example it is too old Change 7 polymer microsphere suspensions um Sheath pressure gives excessively high or low pressure 95 to 105 kPa is acceptable Replace the MP 822V with a new one The flow cell is clogged Remove the cause Dust is adhered to the flow cell outside wall Clean the outside wall of the flow cell with a cotton swab moistened with alcohol 2 14 Service Manual MEK 8222 Scattergram Problems 2 TROUBLESHOOTING the following typical one after aspirating the healthy person s blood 02 99 13 15 A Comp ty FE Granular ity Granular ty 02 FEB 99 IDNO 000000000004 109 1
67. by the first pressure sensor on the UT 7158 SHEATH CONTROL board in the MP 822V sheath pump unit 2 2nd PRESSURE Pressure detected by the second pressure sensor on the SHEATH CONTROL board in the sheath pump unit The normal range of the first pressure sensor 130 to 160 kPa The normal range of the second pressure sensor 80 to 120 kPa 6 19 6 ADJUSTMENT Temperature Sensor 6 20 unit are displayed SENSOR MONITOR CBC MANOMETER HGB SENSOR WBC UP WBC LOW RBC UP RBC LOW CALIBRATION SWITCH MP 820V CBC ELECTRODE MC 820V WBC RBC the heater of the warmer unit The temperature values from the temperature sensors in the ZY 820V warmer 07 JUL 02 14 53 LIQUID SENSOR DETERGENT 0 16 V HEMOLYNAC 3 0 23 V HEMOLYNAC 5 0 21 V SHEATH DILUENT 0 20 V LIQUID TANK 472V SHEATH TANK 415 V SHEATH PUMP 1st PRESSURE 155 kPa 2nd PRESSURE 101 kPa WARMER TEMP 29 INTERNAL TEMP 33 C WARMER TEMP Temperature detected by the temperature sensor on 2 INTERNAL TEMP Temperature detected by the temperature sensor on the WARMER CONTROL board in the warmer unit 8222 6 ADJUSTMENT Setting on the 7159 WARMER CONTROL board The WARMER CONTROL board controls the heater by turning it on or off according to the temperature set by resistance of the thermistor The temperature can be set to 30 C 35 C 40 C or OFF by the DIP switch setting as shown bel
68. d other than the hematology analyzer such as the smear method 2 TROUBLESHOOTING Incorrect Data Background Noise When the obtained diluent data just exceeds the tolerable value of the parameter shown in the table in Background Noise Check of this section there are five possible causes of error 1 Dirty diluent 2 Dirty fluid paths in the instrument NOTE Before storing the instrument for a long time clean all fluid paths in the instrument with distilled water Otherwise the diluent salt adheres to the baths and cannot be removed easily 3 Dust which adheres to the flow cell wall causes the laser beam to widely diverge and scatter 4 Electrical noise affecting the counting calculation circuit AC line peripheral equipment such as microwave treatment machine motors in the instrument 5 Mechanical noise affecting the counting calculation circuit Vibration from motors in or around the instrument such as a centrifuge As countermeasure to the above 5 causes take the following actions Cause 1 Replace the 18 L diluent container with a new one Cause 2 Press the Clean key 2 or 3 times Cause 3 Gently clean the flow cell wall with a cotton swab moistened with alcohol and leave it to dry CAUTION Never scratch the flow cell wall Cause 4 Securely ground the instrument including any optional units such as a card printer Cause 5 Keep the instrument away from the vibration sou
69. d to the 4WAY LIQUID SENSOR board Cable connected to the JQ 822V center piping unit 2 Cables connected to the MP 821V sample pump unit 2 Cables connected to the MASTER board Front view Service Manual MEK 8222 4 DISASSEMBLY AND ASSEMBLY 4 Remove the 2 black screws which secure the flow cytometry piping unit to the chassis Remove the unit from the instrument Rear view Service Manual MEK 8222 4 13 4 DISASSEMBLY AND ASSEMBLY Removing the MP 520V Pump Unit Front view RBC pump unit 1 Remove the 2 tube joints from the pump unit 2 Disconnect the cable connected to the DRIVER2 board at this board 3 Remove the 3 black screws which secure the pump unit to the chassis through the shield cover 4 Remove the pump unit from the instrument WBC pump unit 1 Remove the 2 tube joints from the pump unit 2 Disconnect the cable connected to the DRIVER2 board at this board 3 Remove the 3 black screws which secure the pump unit to the chassis through the shield cover 4 Remove the pump unit from the instrument 5 part WBC pump unit 1 Remove the 2 tube joints from the pump unit 2 Disconnect the cable connected to the DRIVERI board at this board 3 Remove the 3 black screws which secure the pump unit to the chassis through the shield cover 4 Remove the pump unit from the instrument 4 14 Service Manual MEK 8222 Section 5 Socket Pin Assignment Socket Pit ASSIGRmOeDt orto
70. e Manual MEK 8222 1 GENERAL Inside Panels CAUTION e For WBC 5 part differential measurement the laser beam is used Do not open any part labeled CAUTION The laser can cause burns and blindness e Do not remove the caution labels XE IC BE 6 75 25510 9 L PHACED E LF BHA CAUTION LASER RADIATION WHEN OPEN AND INTERLOCKS DEFEATED AVOID EXPOSURE TO BEAM CLASS 1 LASER PRODUCT e When attaching the filter joint assembly be careful not to bend or damage the filter packing at the bottom of the measurement bath e When there is a leakage check that there is no scratch or damage to the circumference of the filter Service Manual MEK 8222 1 11 1 GENERAL Composition MEK 8222J MEK 8222K Not available JQ 820V Inlet Outlet Unit XP 513V 3 way Valve JQ 821V Left Side Piping Unit XP 513V 3 way Valve JQ 822V Center Piping Unit UT 7171 LIQUID LEVEL SENSOR Board XP 513V 3 way Valve JQ 823V 4 channel Liquid Sensor Unit UT 7179 4WAY LIQUID SENSOR Board MB 820V Sub Bath Unit MC
71. e front cover HGB sensor adjustment 2 Remove the HGB measuring block cover 3 Call up the SENSOR MONITOR screen 4 Adjust the HGB sensor output control VR until the HGB SENSOR ON value shows 3 0 0 1 V when the WBC measurement bath is filled with diluent SENSOR MONITOR CBC MANOMETER LIQUID SENSOR 0 85 V DETERGENT 0 16 V 0 46 V HEMOLYNAC 3 0 23 V 0 91 V HEMOLYNAC 5 0 21 V 0 23 V SHEATH DILUENT 0 20 V MP 820V LIQUID TANK 472V CBC ELECTRODE MC 820V SHEATHTANK 415 V WBC WBC 17 9 V RBC SHEATH PUMP RBC 17 9 V 1st PRESSURE 155 kPa 2nd PRESSURE 101 kPa WARMER TEMP 29 C INTERNAL TEMP 33 C Service Manual MEK 8222 6 11 6 ADJUSTMENT HGB measuring block cover HGB sensor output control VR Calibration Switch The correction factors of the CBC measuring unit and complex pump unit are displayed MP 820V Correction factor which is set by the rotary switches of the complex pump unit MC 820V WBC Correction factor which is set by the rotary switches of the WBC measuring block of the CBC measuring unit MC 820V RBC Correction factor which is set by the rotary switches of the RBC measuring block of the CBC measuring unit MP 820V Rotary switches MC 820V Rotary switches Rotary switches 6 12 Service Manual MEK 8222 6 ADJUSTMENT Liquid Sensor The output voltage from each liquid sensor is displayed a CBC Dit 07 JUL 02 14 53 CBC MANOMETER HGB SENSOR LIQUID SENSOR WBC UP 0
72. e is firmly attached e The measurement baths and sample cup are clean If the scattergram count is still over 100 refer to the Background Noise section 2 3 2 TROUBLESHOOTING Scattergram Check Counting Blood Samples 1 Reduce the background noise so that the scattergram count TOC is less of a Healthy Person than or equal to 100 2 Prepare a blood sample of a healthy person The blood sample must be within 8 hours after the blood sample is collected 3 Turn the sample tube containing the blood sample upside down ten times or more so that the plasma and red blood cells are evenly mixed 4 Setthe sample tube containing the blood sample in the rack position no 1 5 Press the Start key to count the blood sample 6 Check that the 5 parts of WBC i e neutrophil lymphocyte monocyte eosinophil and basophil are scattered on each allotted area as shown below Ifa part of the 5 part WBC is scattered on an area different from the allotted area refer to Section 6 Adjustment 02 99 13 15 O2 FEB 99 DNO 000000000004 9 WBC 80 10 uL PEDE NE 597 47 462 10 uL ae Es LY 3L5 25 145 g dL 59 0 5 432 2 0 02 MCV 93 5 fL em BA 09 01 314 pg ops MCHC 336 g dL y PLT 238 10 uL Comp lexi ty Granularity RDW 1141 RB sl PLT PDW 183H 5 pta larity 100 200 2 10 20 2 4 Service Manual
73. e pump unit See Fig 11 The following problems can cause the failure that the pump does not work The piston position sensor photo interrupter is broken The piston rod coupler screw is loose There is a poor contact between the connectors or the cable is disconnected The following problems can cause the failure that the pump works but the blood sample is not transferred The electromagnetic valve is broken leak wire disconnection poor contact The piston O ring has deterioration Tube joints at the electromagnetic valve are loose Leak at the end of the tube Remove the cause Service Manual MEK 8222 2 21 2 TROUBLESHOOTING Possible Causes Countermeasure Failure of MP 820V See Fig 13 The following problems can cause the display of too many ghosts on the scattergram Faulty piston position sensor does not allow the hemolysing pump to dispense the correct volume Remove the cause ar Faulty electromagnetic valve does not allow the hemolysing pump to aspirate or dispense the correct volume Faulty O ring faulty piston or faulty cylinder allows the hemolysing reagent to leak 822 failure No flow of the sheath fluid diluent See Fig 14 Remove the cause The following problems can cause such a scattergram Poor contact at the connector or disconnection of the cable Aleak in the fluid path or tube disconnection
74. emory Card 1 Turn off the main power switch on the rear of the instrument 2 Remove the right side cover as shown below Right side cover Main power switch 3 Insert the memory card into the slot as shown below 4 Turn on the main power switch on the rear of the instrument 1 24 Service Manual MEK 8222 Service Manual MEK 8222 5 6 1 GENERAL Turn on the Power key on the front panel of the instrument The DOWNLOAD MEK 8222 PROGRAM screen appears The MEK 8222 software has five parts MASTER BD board SLAVE CBC BD board SLAVE MD BD board LANGUAGE ENGLISH local language program ANALYSIS PROGRAM DOWNLOAD MEK 8222 PROGRAM V01 02 MEMORY CARD CHECK SUMI MASTER BD Vo1 01 4649 SLAVE CBC BD V01 01 DOWNLOAD SLAVE MS BD V01 01 ALL PROGRAM LANGUAGE ENGLISH 01 01 ANALYSIS PROGRAM V01 01 0130 DOWNLOAD SEPARATERY MASTER BD V01 01 4649 SLAVE CBC BD V01 01 C53D LANGUAGE ENGLISH V01 01 2F11 ANALYSIS PROGRAM V01 01 0130 SLAVE MS BD 01 01 2 Install the software as follows To install all the software press the DOWNLOAD ALL PROGRAM touch screen key To install the MASTER BD software only press the MASTER BD Vxx XX xxxx key on the screen To install the SLAVE CBC BD software only press the SLAVE CBC BD Vxx xx xxxx key on the screen To install the SLAVE MS BD software only press the SLAVE MS BD Vxx xx xxxx key on
75. en key SERVICE MAINTENANCE screen appears 5 Press the MONITOR touch screen key 6 Press the SENSOR MONITOR touch screen key The SENSOR MONITOR screen appears SENSOR MONITOR CBC Diff 07 JUL 02 14 53 CBC MANOMETER HGB SENSOR LIQUID SENSOR WBC UP 0 85 V 244 V DETERGENT 0 16 V WBCLOW 046V 0 26 V HEMOLYNAC 3 0 23 V RBC UP 0 91 V HEMOLYNAC 5 0 21 V RBCLOW 0 23 V CALIBRATION SWITCH SHEATH DILUENT 0 20 V MP 820V LIQUID TANK 472V CBC ELECTRODE MC 820V SHEATH TANK 415 V WBC WBC 17 9 V RBC SHEATH PUMP RBC 17 9 V 1st PRESSURE 155 kPa 2nd PRESSURE 101 kPa WARMER TEMP 29 C INTERNAL TEMP 33 C Service Manual MEK 8222 6 7 6 ADJUSTMENT CBC Manometer 6 8 The output voltages from the sensors attached to the two manometers in the CBC measuring unit are displayed 1 WBC UP Output voltage of the sensor located at the upper position of the WBC manometer 2 WBC LOW Output voltage of the sensor located at the lower position of the WBC manometer 3 RBC UP Output voltage of the sensor located at the upper position of the RBC manometer 4 RBC LOW Output voltage of the sensor located at the lower position of the RBC manometer SENSOR MONITOR CBC MANOMETER HGB SENSOR WBC UP 0 85 V WBCLOW 046V RBCUP 0 91 V RBC LOW 0 23 V CALIBRATION SWITCH MP 820V MC 820V WBC RBC CBC ELECTRODE 07 JUL 02 14 53 LIQUID SENSOR DETERGENT 0 16 V HEMOLYNAC 3 0 23 V HEMOLYNAC 5 0 21 V
76. equired part is located This will help us to promptly attend to your needs e Always use parts and accessories recommended or supplied by Nihon Kohden Corporation to assure maximum performance from your instrument Service Manual MEK 8222 1 GENERAL Specifications Measured Parameters Ranges and Reproducibility to Specimen from Venous Blood Specifications were determined using hematology control blood MEK 3DN counted 10 times consecutively Reproducibility to Specimen from Venous Measured Parameters Measuring Range Blood CV Coefficient of Variation WBC White blood cell count 0 to 300 x 10 uL within 2 0 CV 4 0 to 9 0 x 10 uL Neutrophil percent 0 to 99 9 BC 4010 9 0 x 107 LY Lymphocyte percent 0 to 99 9 BC 4 0 to 9 0 10 0 Monocyte Gin 9 9 Within 1209 WBC 4010 90 107 EO Eosinophil percent 0 to 99 9 5 RUN WBC 4 0 to 9 0 x 1041 E umm m t NE Neutrophil count 0 to 99 9 x 10 uL LY Lymphocyte count 0 to 99 9 x 10 uL MO Monocyte count 0 to 99 9 x 10 uL EO Eosinophil count 0 to 99 9 x 10 uL BA Basophil count 0 to 99 9 x 10 uL RBC Red blood cell count 0to14 9x 10 uL within 1 5 CV 5 0 x 1071 HGB Hemoglobin concentration 0 to 29 9 g dL within 1 5 CV 16 g dL HCT Hematocrit 0 to 99 9 Mean cell volume 20 to 199 fL within 1 0 CV 70 to 120 fL MCH Mean cell hemog
77. hat WBC counts are inaccurately obtained from a patient with specific conditions Especially the red blood cells from a 1 patient who has hepatopathy certain special treatment or is neonatal with high resistance of their membranes against hemolysing reagent will have insufficient hemolysing This will cause too many ghosts on the scattergrams It requires a measurement method other than the hematology analyzer such as the smear method 2 The operating temperature is out of 15 to 30 C Use the instrument within the range of 15 to 30 according to the specifications If aspirating the blood sample takes a longer time than Remove the cause the usual aspiration time or stirring the blood sample in the mixing chamber is insufficient hemolysing the RBC insufficiently can result in a lot of ghosts The possible causes are shown below 3 Clog in filter Deterioration of pump tube at rotary pump unit MP 520V Loose tube joint Clog at sampling cup Leak at mixing chamber especially at joint Leakin fluid path The lyse pump unit has one of the following problems Remove the cause The hemolysing reagent is not dispensed correctly because of abnormal position sensor 4 The hemolysing reagent is not aspirated dispensed correctly because of an abnormal valve The hemolysing reagent leaks because of an abnormal O ring piston or cylinder 2 18 Service Manual MEK 8222 e Scattergram 3 2 TROUBLES
78. he AUTO GAIN key to change the current gain to the target gain Service Manual MEK 8222 6 3 6 ADJUSTMENT Adjusting the FS FL SD Gain and FS Threshold Before performing this adjustment check that the CV values of FS and FL meet the following conditions by measuring the 7 um polymer microsphere suspensions FS lt 7 FL lt 7 Refer to Adjusting the Flow Cell Position 1 Press the ADJUST GAIN FINE key to display the DIFF GAIN FINE Screen The For fine adjustment human blood must be measured Go to READY screen measure blood and return to this screen Go to READY screen message is displayed 2 Press the YES key and go to the READY screen 3 Setthe 10 human blood samples which were prepared in step 4 in the rack and measure them Make sure that CBC Diff is selected in the PARAMETERS box on the READY screen The samples can also be measured manually 4 Display the DIFF GAIN FINE screen DIFF GAIN FINE CBC Dift 04 AUG 02 14 53 SCATTERGRAM F F F 5 5 5 50 50 CURRENT ACTUAL PEAK AVERAGE ACTUAL PEAK IDEALPEAK 185 53 25 CURRENT SAMPLE SEQH 0000000 ADD TO LIST NEW SAMPLE SEQ 0001123 LOAD NEW ENABLE SEQ 0001100 0001123 SCATTERGRAM GAIN 5 80 FLGAIN 65 SOGAIN 64 sm OOIB 5 AUTO GAIN PRINT 6 4 8222 6 ADJUSTMENT 5 Press the LOAD NEW SC
79. he following operating conditions Around instrument Diluent temperature 15 to 30 temperature 15 to 309 humidity 30 to 9096 atmospheric pressure 850 to 1060 hPa If the temperature is below 15 C the WBC count may increase because the RBCs are not sufficiently hemolyzed due to the lower temperature Insufficiently hemolyzed RBCs will be included in the WBC count as RBC ghosts When the sample diluent or hemolysing reagent temperature is lower than 15 C the scattergram has ghosts in the lymphocyte area 02 99 13 15 02 99 IDNO 000000000004 1 10 1 WBC 77 10 1 55 9 4 4 4 59 106 LY 328 25 140 g dL 52 0 4 3551 EO 32H 02 7731 fL BA 29H 02 MCH 30 5 MCHC 39 H g dL PLT 320 10 RDW 1091 Complexity granularity PCT 047 2 MPV 55 fL PDW 193H Granular ity When the sample diluent or hemolysing reagent temperature is higher than 30 C it will cause too many ghosts on the scattergrams Too many ghosts will decrease the count of white blood cells The 5 parts of the white blood cells are not clearly separated on the scattergrams The percent of eosinophil may be higher than the normal temperature range 02 99 13 15 02 FEB 99 DNO 000000000004 10 1 WBC 48 103 uL NE 374L 1 RBC 5 16 10 LY 332 16 150 g dL MO 53 0 3 HCT 460 240H 12 89 1 fL BA 01 0
80. he requirements of the IVD Directive 98 79 EEC This equipment complies with International Standard EN55011 1999 Group 1 Class B Class B EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly connected to a low voltage power supply network which supplies buildings used for domestic purposes Service Manual MEK 8222 i This page is intentionally left blank Service Manual MEK 8222 Conventions Used this Manual and Instrument Warnings Cautions and Notes Warnings cautions and notes are used in this manual to alert or signal the reader to specific information WARNING A warning alerts the user to the possible injury or death associated with the use or misuse of the instrument CAUTION A caution alerts the user to possible injury or problems with the instrument associated with its use or misuse such as instrument malfunction instrument failure damage to the instrument or damage to other property NOTE A note provides specific information in the form of recommendations prerequirements alternative methods or supplemental information Service Manual MEK 8222 iii Explanations of the Symbols in this Manual and Instrument The following symbols found in this manual instrument bear the respective descriptions as given On panel Symbol Description Symbol Description Main power lamp HEMO 3 HEMOLYNACe3 hemolysing reagent
81. he semiconductor laser can cause each part of the WBC to not be in the allotted area and each cell size to be in a lower position than the actual WBC See Fig 1 Replace the MO 820V laser optical unit with a new one If the flow cell is set to a wrong position so that the center of the flow cell is slightly out of alignment with the laser beam axis the scattergram may show that each cell size is in a lower position than the actual WBC because the wrong position of the flow cell decreases the scattered light intensity See Fig 1 Adjust the flow cell position Refer to Section 6 Adjusting the Flow Cell Position Service Manual MEK 8222 2 15 2 TROUBLESHOOTING 2 16 25 JUL 93 15 33 tH 0 25 JUL 99 IDNO KOHDEN 8997 8551 WBC 4 7 10 uL 2 4 RBC 5 04 10 uL 1 6 HGB 15 2 g dL 20 3 js 4 MCU MCH pg g dL PLT 18 uL IO CJ Granular ty 08 01 09 31 Comp lexi ty E MCHC 3 ranularity RDM PCT MPU PDW G lt Fig 1 gt 08 FEB 1 IDNO OLDBLOOD 0001 x 105 01 WBC 7 0 103 uL 5 1 RBC 5 32H 10 uL HGB 15 9 g dL HCT 48 7 k MCU 91 5 fL MCH 29 9 pg MCHC 32 6 g dL PLT 223 10 uL Granularity RDW 11 5 PCT MPU PDW Granularity lt Fig 2 gt When the blood sample is left for more than 12 hours after the collection the 5 parts of white blood cells have not been clearly separated Service
82. here is a leak in the fluid path or a tube is folded There is foreign matter such as dust in the flow cell There are bubbles in the flow cell The straightening part of the flow cells is clogged by contamination Dustor contamination clogs somewhere in the fluid path Foreign matter such as dust is adhered to the flow cell outside wall Foreign matter such as dust is adhered to the lens Foreign matter such as dust is adhered to the detector MO 820V has one of the following problems Laser beam is thinner than the normal Laser beam axis is not aligned with the detector MP 822V has the following problem Since the sheath pump gives excessive high pressure the sheath flow is turbulent If the sheath pump gives excessive low pressure the WBC cannot be kept in single file at the flow cell See Fig 5 Remove the causes Refer to operator s manual Section 10 Cleaning the Flow Cell Refer to operator s manual Section 10 Cleaning the Flow Cell Replace the MO 820V with one Adjust the secondary pressure to 95 to 105 kPa using the regulator a new Service Manual MEK 8222 2 19 2 TROUBLESHOOTING 28 11 99 20 08 28 99 98JUL99 0130 ia x uL WBC 14 6 18 uL Em 87 2 RBC 4 20 10 1 LY 8 8 3 3 g dL 2 j CU 96 0 fL 0 2 j o prc g dL 10 uL 00 Comp ty Granular
83. in WBC will cause excessive light scatter In these cases measure manually Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer 2 Increased lipids The blood sample may be milky when there is excessive lipid This may occur with hyperlipidemia hyperproteinemia and hyperbilirubinemia Accurate HGB measurement can be achieved by manual methods and a plasma blank 3 Increased turbidity When RBC are resistant to lysing turbidity may increase causing increase in HGB Observe if MCH and MCHC values are abnormal HGB result affects MCH and MCHC result 4 Fetal bloods The mixing of fetal and maternal blood may increase HGB value HCT Agglutinated RBC RBC agglutination may cause erroneous HCT and MCV values Observe if MCH and MCHC values are abnormal In such a case measure manually 1 4 Service Manual MEK 8222 RDW PLT MPV 1 GENERAL Agglutinated RBC RBC agglutination may cause erroneous HCT and values Observe if MCH and MCHC values are abnormal In such a case measure manually Excessive number of large PLT Excessive number of large PLT and or excessively high WBC may affect the MCV value Check by careful examination of the stained blood film MCH is determined from HGB and RBC values Therefore the limitations for HGB and RBC also affect MCH value is determined from HGB and HCT values Therefore the limitations for HGB and HCT also affect
84. lay unit displays the numeric and graphic data and operation keys on the color LCD screen This unit controls the LED indicators for the status of the switches on the front panel Service Manual MEK 8222 MASTER Board 3 BOARD UNIT DESCRIPTION The MASTER board controls the operations for the measurement with flow cytometry method and performs the data processing for the user interface as shown below SLAVE CBC Board Control of the electromagnetic valves and motors Amplification and count of the pulses obtained from the scattered light LCD control USB control Memory card control The SLAVE CBC board controls the following operations for dilution and blood cell count with the electrical resistance detection method SLAVE MS Board Control of the electromagnetic valves and motors Amplification and count of the pulses obtained from the electrical resistance detection Dilution control The SLAVE MS board controls sample transportation stirring and dispensing such as control of the electromagnetic valves and motors Service Manual MEK 8222 3 3 Section 4 Disassembly and Assembly Removing the PV 820V Display 04 18 8 4 1 Removing the MC 820V CBC Measuring Unit essen 4 2 Removing the MB 820V Sub Bath nennen 4 4 Removing the MP 820V Complex Pump 2 4 5 Removing the MS 820V Closed Sampler Unit
85. lobin 10 to 50 pg MCHC Mean cell hemoglobin concentration 10 to 50 g dL RDW Red blood cell distribution width 0 to 50 PLT Platelet count 0 to 1490 10 uL within 4 0 CV 3 0 x 10 8 PCT Platelet crit 0 to 2 9 MPV Mean platelet volume 0 to 20 0 fL PDW Platelet distribution width 0 to 50 Detection Method Blood cell count Electrical resistance detection Hemoglobin Cyanmethemoglobin optical detection Hematocrit Histogram calculation WBC population Light scatter by laser Platelet crit Histogram calculation RBC distribution width Histogram calculation Platelet distribution width Histogram calculation Standardization Analysis Method WBC ICSHI988 ICSH The assignment of values to fresh blood used for calibrating automated blood cell counters Clin Lab Haematol 10 203 212 1988 RBC ICSH1988 ICSH The assignment of values to fresh blood used for calibrating automated blood cell counters Clin Lab Haematol 10 203 212 1988 Service Manual MEK 8222 1 3 1 GENERAL HGB 5 15 2 15 2 Reference and Selected Procedures for the Quantitative Determination of Hemoglobin in Blood Second Edition Approved Standard 1994 HCT NCCLS 7 2 H7 A2 Procedure for Determining Packed Cell Volume by the Microhematocrit Method Second Edition Approved Standard 1993 PLT Brecher amp Cronkite Interference Substances WBC Unlysed red cells In some rare occasions the RBC in the blood sample may
86. ly performed and the READY screen is displayed Stops operation when pressed during operation Returns to the READY screen when pressed 3 Reset key while changing settings Use this key only when an error occurs 4 Manual count For manual mode only switch Press to aspirate the sample and start counting 5 Manual sampling For manual mode only nozzle Aspirates the sample 6 Power lamp Lights when the main power switch on the rear panel and power key on the front panel are turned on 7 Laserlamp Lights when the laser switch on the right side panel is turned on For manual mode only 8 Operation indicator When blinking Aspirating the sample lamps When off Counting the sample When lit Ready for next counting 9 Manual mode key Sets the measurement mode in manual mode when pressed The sampling nozzle for manual mode is lowered 10 LCD screen Displays measurement data various messages and touch screen keys For rack mode only BY Press this key to slide the sample rack in or out For rack mode only He Press this key to start measurement 13 Emergency key Press this key to count an emergency sample during routine counting Cleans the fluid path aperture and manometer with detergent Priming is performed 14 Clean key automatically after cleaning the fluid path Press this key when clogging occurs the manometer becomes dirty or bubbles occur in the manometer 15 Sample rack Holds the sample tubes containing specimens
87. nt and or system The following describes some common interference sources and remedial actions 1 Strong electromagnetic interference from a nearby emitter source such as an authorized radio station or cellular phone Install the equipment and or system at another location if it is interfered with by an emitter source such as an authorized radio station Keep the emitter source such as cellular phone away from the equipment and or system 2 Radio frequency interference from other equipment through the AC power supply of the equipment and or system Identify the cause of this interference and if possible remove this interference source If this is not possible use a different power supply 3 Effect of direct or indirect electrostatic discharge Make sure all users and patients in contact with the equipment and or system are free from direct or indirect electrostatic energy before using it A humid room can help lessen this problem 4 Electromagnetic interference with any radio wave receiver such as radio or television If the equipment and or system interferes with any radio wave receiver locate the equipment and or system as far as possible from the radio wave receiver If the above suggested remedial actions do not solve the problem consult your Nihon Kohden Corporation subsidiary or distributor for additional suggestions The CE mark is a protected conformity mark of the European Community The products herewith comply with t
88. o CODEO Position Sensor CODE1 Depressure needle UD1 Tum UD3 Depressure needle 904 Sampling nozzle UpandDown Turn Warmer unit ZY 820V 004 VH2P PHD12P PHR5 Complex pump unit pw DC line eum line gt Serial communication line VH2 P 00 Power supply unit SC 820VJ VK Sto 511 swi sw2 RLO 1 2 3 MD CONNECTION board Open sampler unit MS 821V SENSOR board UT 7065 Position sensor Position sensor EOS POS CODEO CODE1 CN301 PHD10P 001 CN302 CNA110 CNAO06 005 VH10P CN 7 02 Ao e Pup 10P ove PHD10P 0501 CINCO VH10P Table unit 228 202 MS 822V PHD20P 60 00 H8S CNoo4 CNOO1 CPU CNAO1 DRIVERS board VH6P VH 6P CNA230 CN201 CN202 CNP231 UT 7154 SLAVE MS board AMP7P CN201 2271 26 red UT 71 48 a DRIVERS board CN101 CN102 Right and
89. ohden specified reagents Use of MEK 3DN Using the MEK 3DN does not allow the data reliability on the scattergram See Fig 9 Not required Failure of diluent or blood sample aspiration See Fig 10 The following problems can cause such a scattergram filter is clogged The pump tube at MP 520V is broken tube joint is loose sampling cup outlet is clogged There is a leak at the mixing chamber Atube in the fluid path has a leak Remove the cause Failure of sample transfer to the flow cell unit from the mixing chamber 11 The following problems can cause such a scattergram The waste fluid tube is collapsed or clogged by dust or contamination flow cell is clogged by contamination The sample needle is clogged at the straightening part in the flow cell The MP 821V does not work The silicon tube is not completely inserted into the bottom of the sample needle under the flow cell or the silicon tube is folded See Fig Remove the cause Failure of flow cell unit MF 820V See Fig 12 The following problems can cause such a scattergram The flow cell is broken flow cell is clogged by contamination or dust The straightening part of the flow cell has a leak or clog Foreign matter such as dust is adhered to the flow cell outside wall Remove the cause Replace the flow cell unit with a new one Failure of MP 821V sampl
90. onverted to electrical signals and amplified on the PRE AMP board Complex Pump Unit The complex pump unit aspirates a whole blood sample and dilutes it 200 times and 40 000 times This unit dispenses the constant volumes of the diluent and hemolysing reagent Service Manual MEK 8222 3 1 3 BOARD UNIT DESCRIPTION Sample Pump Unit Sheath Pump Unit The sample pump unit aspirates the diluent and dispenses it This unit sends the diluted and hemolysed sample to the flow cell at 3 to 6 ul second The sheath pump unit applies the constant pressure to the diluent and sends it to the flow cell Closed Sampler Unit Open Sampler Unit Table Unit Display Unit 3 2 The closed sampler unit releases a pressure inside the sample tube with the cap pierce nozzle The unit aspirates the sample in the tube and moves the sampling nozzle to the sub bath or sampling cup so that the aspirated sample is ready for the measurement After the sample is aspirated the rinse unit cleans blood from the outside of the sampling nozzle The open sampler unit moves the manual sampling nozzle to the sub bath or measurement bath so that the aspirated sample is ready for the measurement After the sample is aspirated the manual rinse unit cleans blood from the outside of the sampling nozzle The table unit moves a sample tube set in the rack to the stirring position or blood aspiration position using x axis and y axis drive motors The disp
91. ow DIP switch AAT WARMER CONTROL Sy 01 uT 7159 TEMPERAT ET ES 2o o o J 71536 979 00006 09118 switch setting Set Temperature BIT 1 BIT 2 BIT 3 BIT 4 30 OFF OFF 39 C OFF ON OFF OFF 40 C OFF OFF ON OFF Heater OFF OFF OFF OFF ON The DIP switch is set to 40 C at the factory Service Manual MEK 8222 6 21 6 ADJUSTMENT Calibrating the Touch Screen 1 Press the Manual mode key while pressing the reset key The TOUCH SCREEN CALIBRATION screen appears Manual mode key TOUCH SCREEN CALIBRATION Press the exact center of the mark 2 Touch the cross point of the mark at the upper left corner of the LCD Immediately after touching the touch point the instrument generates a pip tone and the mark moves to the upper center point of the LCD TOUCH SCREEN CALIBRATION Press the exact center of the mark 6 22 Service Manual MEK 8222 8222 6 ADJUSTMENT Touch the cross point of the mark of the LCD whenever the mark moves to a different position In the last touch the cross point of the mark at the lower right corner of the LCD The screen changes to the READY screen which shows you that the touch screen calibration is completed TOUCH SCREEN CA
92. owing problems require adjustment of the flow cell position e When a blood sample collected from a healthy person is counted the scattergram shows that the 5 parts of WBC are not clearly separated e When the 7 um polymer microsphere suspensions are counted in the Particle Measurement mode one of the CV values of FS and FL stays in the following percentage of FS Higher than 7 CV of FL Higher than 7 Adjust the flow cell position rough adjustment of the gain for WBC 5 part differential measurement by measuring 7 um polymer microsphere suspensions The DIFF GAIN screen can only be entered when the type of operator is lab technician or manufacturer Observe the instructions on the 7 Um polymer microsphere suspensions manual to achieve optimum performance and safety CAUTION e Do not swallow the polymer microsphere suspensions If swallowed contact your physician immediately e Avoid contact with the mouth and eyes If the polymer microsphere suspensions contacts the mouth or eyes wash thoroughly and immediately with water and contact your physician immediately 1 Remove the flow cell unit window cover on the right side panel by removing two screws 2 Loosen the screw fastening the flow cell unit adjustment screw with the thick hex wrench Turn the screw counterclockwise 6 1 6 ADJUSTMENT 3 Insert the thin hex wrench into the flow cell adjustment screw Flow cell unit adjustment screw
93. p lexity Granularity RDW EE EE PCT MPU 5 PDW Grant Lar tty Fig 12 WBC RBC HGB HCT MCU MCH PLT Granularity RDW MPU PDW 5 7 Fig 13A MCHC 7 3 10 uL 5 91 15 8 g dL 47 3 k 90 8 30 3 33 4 236 11 40 8 21 9 2 19 8H 29 MAR BO 12 32 adi oa IDNO No LYSE 0001 T 6 7 10 uL 5 42H 19 uL 15 9 g dL 48 5 89 5 fL 29 3 MCHC 32 8 gt 8 4 257 180 uL High membrane resistance of the red blood cells collected from the patient who has a disease such as liver disease or has red blood cells resistance to hemolyzation allows such a scattergram As another case since there is no hemolysing reagent in the mix chamber due to the failure of the hemolysing pump the data is scattered around the upper right corner on the scattergrams Then some message flags such as Immature GR will be displayed 2 25 2 TROUBLESHOOTING 9 MAR Q 13 12 Comp lexi ty Granularity RBI 9 MAR 28 IDNO LYSE ERR 2801 3 WBC 7 0 180 uL RBC 5 42H 18 uL HGB 15 9 g dL HCT 48 0 x MCU 90 2 MCH 29 3 MCHC 32 5 PLT 271 RDW 12 8 0 29 MPU 10 7 PDW 19 9H Fig 13B High membrane resistance of the red blood cells collected from the patient who has a disease such as liver disease or has red blood cells re
94. pull the rear cover toward you until the cable connected to the rear cover backside appears as shown below Disconnect the cable at the INTERFACE board which is attached to the rear cover Completely remove the rear cover from the instrument 4 Inserta small flat blade screwdriver between the ROM and its socket on the MASTER board as shown below Carefully lift the ROM with the screwdriver until the ROM slightly tilts Take out the screwdriver and insert it into the opposite side of the IC socket Continue lifting both sides of the ROM with the screwdriver until the ROM 15 removed MASTER board Flat blade screwdriver 1 22 Service Manual MEK 8222 Service Manual MEK 8222 1 GENERAL CAUTION When you remove the ROM from the IC socket with a sharp edge tool such as a flat blade screwdriver be sure that it does not damage the printed pattern under the IC socket mm set s n a a a a B m TTT Wet T m ape Am 1 M os 373 A 5 Putthe new ROM on the IC socket and carefully insert the pins of the new ROM into the IC socket CAUTION e Before putting the new ROM on the IC socket check that the ROM position is the same as the old ROM e Do not break or bend a pin of the ROM The pins must be correctly inserted into the socket Otherwise the instrument may not work 1 28 1 GENERAL Upgrading the Software with the M
95. rce 2 12 Service Manual MEK 8222 Service Manual MEK 8222 2 TROUBLESHOOTING How to Decrease the Background Noise on the Instrument after Long Term Storage If the instrument is left for a long time without diluent draining the diluent in the measurement bath evaporates The salt crystals remain and adhere to the mixing chamber sampling cup and other fluid paths The salt crystals cannot be removed with normal cleaning High background noise results from storing the instrument without cleaning it Even an instrument cleaned with distilled water has dust which is very difficult to remove after long term storage To decrease the background noise on the instrument perform the following procedure 1 Clean the sampling cup with the CLEANAC 3 detergent Refer to Section 10 Maintenance of the operator s manual 2 Check the filters Refer to Section 10 Maintenance of the operator s manual 3 Check the pump tube and pinch valve tubes Refer to Section 10 Maintenance of the operator s manual 4 Press the Clean key on the front panel to clean the inside of the instruments 5 Setafew empty sample tubes in the rack and perform the background noise check to check if the background noise on specified parameters are within the respective normal ranges NOTE If the background noise is still out of the range repeat the above procedure 2 13 2 TROUBLESHOOTING Laser Optical Unit Problems Pro
96. refer e 1 1 EE 1 2 Specifications x c 1 3 Measured Parameters Ranges and Reproducibility to Specimen from Venous Blood 1 3 DeTECHON 1 3 Standardization Analysis Method 1 3 5 nerit ete ostuce ege nica d Leda arua Das uda 1 4 Dilution Em 1 6 Co ntirig TIME buta ch decks pena 1 6 1 6 Data SORA GS 1 6 Environmental 1 6 Power Requirements eed tur d e gel Rui 1 6 Dimensions and Decet eda 1 7 Electromagnetic Compatibility svissneski 1 7 1 7 2 E 1 8 ee E 1 8 Right Side Panel ssie lE 1 9 Rear Panel 1 10 1 11 eese EINE 1 12 Installation Flow Chart iioi I ERR naue sc KT EET ay 1 15 How to Upgrade the 1 21 Replacing the ROM i bre Ern Run Lex 1 21 Upgrading the Software with the Memory
97. s oe e tere fien Rea E aise exa eva Doa EE dnce P eds 6 7 SENSOR MONITOR screen sssssssssssseeeeeee eene nennen nnne 6 7 How to call up the SENSOR MONITOR screen 6 7 CBG tcr oU ce Pede aano ds 6 8 WBC manometer upper sensor 2 6 9 WBC manometer lower sensor 2 6 9 RBC manometer upper sensor 6 9 RBC manometer lower sensor 6 9 CBCO Elect 6 10 SSMS OM M DP 6 11 sensor adjustment iste terere bo cepa cct bete ce 6 11 Calibration S WII coeunt 6 12 Misure 6 13 4 channel liquid sensor unit 6 15 Pressure Sensor ssssssssssssssesseseeenen nennen nnne nnns ener 6 19 Temperature Sensor 6 20 Setting on the UT 7159 WARMER CONTROL board 6 21 Calibrating the Touch Screen ssssssssssssssesssseseeeeeennene enne nnne nnne nns 6 22 Service Manual MEK 8222 6C 1 6 ADJUSTMENT Adjusting the Flow Cell Position Service Manual MEK 8222 The foll
98. sampler unit from the instrument CAUTION in Assembling the Instrument Put the closed sampler unit back to the original position and move the unit to the left as much as possible Fasten the unit to the chassis with the black screws 4 6 Service Manual MEK 8222 4 DISASSEMBLY AND ASSEMBLY Removing the MS 821V Open Sampler Unit The open sampler unit consists of the following Photo sensors Stepping motor and lead screw SENSOR board Rinse unit 1 Disconnect the 2 cables connected to the DRIVER4 board at this board Front view 2 Remove the black tube joint No 9 from the closed sampler unit 3 Remove the 3 black screws which secure the open sampler unit to the chassis 4 Remove the open sampler unit from the instrument CAUTION in Assembling the Instrument Put the open sampler unit back to the original position and move the unit to the left as much as possible Fasten the unit to the chassis with the black screws Service Manual MEK 8222 4 7 4 DISASSEMBLY AND ASSEMBLY Removing the MS 822V Table Unit The table unit consists of the following SENSOR3 board TABLE SENSOR board DRIVERS board Stepping motors Linear slide 1 Remove the 3 screws which secure the table unit to the chassis as shown below Front view 2 Slowly remove the table unit from the instrument until you can disconnect the 2 cables connected to the SLAVE MS board at the table unit
99. sistance to hemolyzation allows such a scattergram As another case since there is insufficient hemolysing reagent in the mix chamber due to the failure of the hemolysing pump the scattergram shows few data and many ghosts which have been counted as EO 1 14 14 Comp lexi ty Granularity 2 26 97 FEB 81 NE LY Ma EO BA roc 2 65 WWO RS T IDNO NOSHEATH 0001 189 uL WBC 7 4 183 uL 5 0 RBC 5 20 105 uL g dL eon 1 8 0 8 dc Goo Fig 14 Service Manual MEK 8222 Possible Causes and Countermeasures for Poor WBC Reproducibility Service Manual MEK 8222 2 TROUBLESHOOTING If you get a poor reproducibility result even though each scattergram has no problem after performing the Reproducibility Check section consider the following reasons and take the countermeasure to solve the reproducibility problem 1 2 3 4 5 6 Deterioration of blood sample Since the blood sample is left for more than 12 hours after being collected the 5 part WBC counts can cause a poor reproducibility Countermeasure Use the blood sample within 12 hours after collecting it Use of blood sample of which the WBC is less than normal If the blood sample collected from a healthy person has a lower than normal quantity of WBC it can cause poor reproducibility The data reproducibility sta
100. supplied main power voltage correct Turn on the instrument Check with alarm messages e Does the instrument generate any of the following alarms NO DILUENT NO DETERGENT NO LYSING REAGENT Check with 7 um polymer microsphere suspensions Do the CV values of FS and FL meet the following conditions FS 796 FL 796 Yes Background noise check s the result out of the normal range Check with blood samples of a healthy person s the obtained scattergram unacceptable s the data reproducibility poor for all measurement parameters e Is there no flag 2 TROUBLESHOOTING Turn the laser switch on Refer to Check Items before Instrument Check Refer to Troubleshooting an Alarm Adjust the flow cell unit position Refer to Section 6 Adjustment Refer to Background Noise Check and Background Noise Refer to Scattergram Check Refer to Reproducibility Check and Poor Reproducibility Refer to Troubleshooting an Alarm for alarm generation 2 1 2 TROUBLESHOOTING Background Noise Check Measuring Background Noise 2 2 Background noise increases in the following cases Problem Countermeasure Old diluent Germs begin to breed in the diluent 6 months after opening Replace diluent Dust in the diluent container Replace diluent Extremely high or low diluent temperature normal range is 15
101. tistically depends on the quantities of each cell If there are more of one type cells than other type cells the CV is lower than for the other type cells If the CV data of a parameter is higher than the CV value on the specifications it means poor reproducibility of the parameter Countermeasure Use a blood sample of healthy person Use CV compensated according to the WBC quantity Use of unspecified reagents Using a reagent different from the specified one can cause poor reproducibility Countermeasure Use only the specified reagents Faulty MP 821V If there is a leak or a bubble in the MP 821 V it can cause poor reproducibility because the diluted blood sample does not flow normally and the WBC quantity decreases Countermeasure Remove the leak or bubble from the MP 821 V Wrongly diluted blood sample If the diluent is left in the sampling cup or mixing chamber the blood sample is more diluted It can cause poor reproducibility If the waste fluid flows backward and is left in the mixing chamber during the aeration for mixing the blood sample with hemolysing reagent it can cause poor reproducibility Countermeasure Remove the leak or clog which causes the remaining diluent or backward waste flow Noise External noise comes in the noise can be counted as white blood cells and will cause poor reproducibility Countermeasure Remove the cause of the noise 2 27 2 TROUBLESHOOTING Troubleshooting an Alarm Reagent Alarm 2 28
102. to 30 C Adjust diluent temperature to 15 to 30 C The sampling nozzle is clogged and bubbles occur in the sub bath Replace the sampling nozzle with a new one Measurement Count the diluent in closed mode and manual mode In Closed Mode 1 Press the Eject key on the front panel The rack table slides out 2 Seta few empty sample tubes in the rack 3 Press the Start key on the front panel to count the empty samples In Manual Mode 1 Press the Manual mode key on the front panel The sampling nozzle is lowered 2 Press the Manual count switch to count the diluent There is no need to aspirate the diluent from the sampling nozzle Service Manual MEK 8222 8222 2 TROUBLESHOOTING Results The result is displayed on the screen after measurement Make sure that the values are less than or equal to the following values WBC 0 2 xl10 uL RBC 0 05 x10 UL HGB 0 1 g dL PLT 10 10 1 TOC 100 count TOC total optical count This parameter only appears in a background noise measurement Disregard the other parameter values because noise does not affect the other parameters If the values are greater than the above values check the following items press the Clean key to clean the fluid path and recount the diluent e The diluent is clean No bubbles in the diluent e The apertures and aperture tubes are clean e The apertur
103. ub baths are used for 200 times for WBC HGB and 40 000 times for RBC PLT diluted samples respectively The diluted sample in each sub bath is poured into the measurement bath of the CBC measuring unit when the sub bath is rotated CBC Measuring Unit The measuring unit has two measurement baths and manometers Each measurement bath has an aperture The aperture size is different for the two baths WBC and RBC PLT Two electrodes are positioned so that the aperture is in between them When a constant current flows between the two electrodes and blood cells are passing through the aperture the voltage between the two electrodes varies in proportion to the cell size electrical resistance The voltage variation signals such as pulses for passing cells are obtained until the constant volume of diluted sample is aspirated A voltage which is proportional to the hemoglobin concentration is also obtained These data are sent to the SLAVE CBC board Laser Optical Unit The laser optical unit emits a laser beam that is applied to each blood cell in the diluted and hemolysed sample which passes through the flow cell in a single file The cells reflect and scatter the laser according to their size and shape The scattered light is collected in three directions i e forward small angle scattered light component FS forward large angle scattered light component FL and orthogonal scattered light component SD These collected light intensities are c

Removing the MP-520V Pump Unit

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