Cycleave®PCR 呼吸器系感染症起因菌検出キット Ver.2
Contents
1. Epoch Biosciences Inc US20020155484 WO0142505 WO02099141 US10 113 445 US09 876 830 Epoch Biosciences Inc Epoch Biosciences Inc GE A B2. K2 2 L pneumophila th Legionella BOR S pyogenes L pneumophila Legionella FAX ROX ROX Loe S pyogenes ZOHO BREL VY TER B FAX ROX ROX http www takara bio co jp 3 CY214 ll 20 1 4 Positive Control 2 lt 8 9 16
3. 2 33 PCR Positive Control fay Probe Primer Mix 1 OO LytA 319 bp 205 bp eae Probe Primer Mix 2 OD MAJENA 16S rRNA 167 bp 110 bp ies a aa TEA Probe Primer Mix 3 Ueo SA Oneu monde 16S rRNA 225 bp 185 bp alD2 on Probe Primer Mix 4 PD Ge 16S rRNA 248 bp 165 bp Probe Primer Mix 5 Legionella pneumophila 16S rRNA 387 bp 274 bp A 16S rRNA 317 bp 230 bp Probe Primer Mix 6 Streptococcus pyogenes 0 264 bp 187 bp http www takara bio coJp 11 CY214 VI Thermal Cycler Dice Real Time System 5 pyogenes gt we eeedee oH db
4. PCR Ver 2 Positive Contrl a betes RNA DNA TTTT Q 2 1 F 3 RNase H TTTT 5 re 1 http www takara bio coJp 2 CY214 25 X 10 X 6 1 2X CycleavePCR Reaction Mixture 750 ul 60 2 Probe Primer Mix 1 2 20 ul 10 3 Probe Primer Mix 2 2 20 ul 10 4 Probe Primer Mix 3 2 20 ul 10 5 Probe Primer Mix 4 2 20 ul 10 6 Probe Primer Mix 5 2 20 ul 10 7 Probe Primer Mix 6 2 20
5. C 3 Tel 077 543 6116 Fax 077 543 1977 a http www takara bio co jp NANOGEN http www takara bio co jp 14 CY214 NOTICE TO PURCHASER LIMITED LICENSE L4 Quencher This product is for measurement of amplification detection for research use only in life sciences re search industrial and environmental testing including food industry but excluding bio terrorism and bio warfare non human animal diagnostic testing forensic testing and providing services to third parties who do not use the services for the purpose of a providing patient management or care and in which the results of such services are not included in patient records and b providing bio terrorism or bio warfare testing and specifically excluding without limitation I any human clinical therapeu tic or diagnostic uses and animal clinical and therapeutic uses including any use of the results of any testing performed with any product for patient management or care or the use of the results of the services for patient management or care and Il a
6. Ct SDM Primary curve Ct SDM 3 33 i 2 4 6 OP wna SY Po ad Ciela http www takara bio coJp 13 CY214 VII Morozumi M E Nakayama S Iwata Y Aoki K Hasegawa R Kobayashi N Chiba T Tajima and K Ubukata 2006 Simultaneous detection of pathogens in clinical samples from patients with community acquired pneumonia by real time PCR with pathogen specific molecular beacon probes J Clin Microbiol 44 1440 1446 IX Cycleave PCR Ver 2 CY216 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 0 2 ml 8 str
7. RNase free dH2O 3 YY 5 V 2 IE 1 1 6 DNA o 24 1 1 ul Probe Primer Mix 1 Probe Primer Mix 4 2 1 Probe Primer Mix 5 Probe Primer Mix 6 3 2 1 2 x CycleavePCR Reaction Mixture 12 5 ul 1 X Probe Primer Mixture 2 ul DNA 2 Positive Control 2 1 ul E FILMAK JZK 9 5 ul Total 25 ul x1
8. 20 I EXTRAGEN II 1 200 uwl20 u10 ul 2 PCR Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 1 0 2 ml 8 0 2 ml 8 strip tube individual Flat caps NJ600 Applied Biosystems StepOnePlus Real Time PCR System Applied Biosystems 7500 Fast Real Time PCR System Life Technologies 4 Baseline Auto Threshold Manual StepOnePlus 7500 Fast Real Time PCR System oes IV 1
9. CY21 4 IdRaRa Cycleave PCR Ver 2 Ver 2 v201401Da lt 6 A PCR Ho
10. Filter ROX OWTNICHW CH BEZERRA COBO EA 5 2 PC Selector C lt P gt Positive Control FAM Filter FAM Filter ROX B n i i bg Gr H Pas ia eae 40 E a Eo E Pe i a 10 10 E 72d E ig tt te hh Mw a eM ee i d a2 4 6 i if ii i ii J j H 6 th D e H i li Crea Crab Filter FAM cSUC s3 0g ROE D NEES EA COAT EY Filter ROX O Positive Control ROX Filter FAM Filter ROX ll he i T a gao mn sx E 31 a W 40 Oo 2 a bh i if Mw i ii H ee fb th we ee H Ob Ob g a2 4 6 i if WM i ii H ee tt th ee H Ob li Cobia Croke Filter ROX TEU CSIR HRD EO BB EBA TOS E Filter FAM 5 3 Selector U gt http www takara bio co jp 9 CY214 6 Text Plate Format Scatter Plot 6 1 Analysis Data lt Text Report gt 6 2 Column Result
11. Sample Type NC PC OK OUT Sample Type UNKN Posi Nega Error Filter FAM ample Type Target Hame Sarnple Haren Filter CHCP CSDM Detected F PM Result FY Oetecied CP Pid msi CP Dainese SDM PAA Result BOR HE bAips HE FAM OK a OR UNEN Hip hic PE F 3442 34 35 Posi Posi Posi PE Hlp Mig PE FAM Z207 21 99 DR JE OK PE Hli LIBS PU FAM OL OL OL Filter ROX Db Type Target Name Sample Mame Filter CEs CSD DesectedF Pii Pee tuh Fy Detected OP Fihi Result chy Detected SOM Patel Fte tuh GDM L 165 piny OK OK OK LIRR L ThS Plie FP RACO Mrz HG7 i Paji i Pos Paci FC Li6s hip FC Ax aT aT IT FC Li6s LISP ROX 1022 17 29 i OK i OK Qik Positive Control 5 FAM 4 Sample Name Mip PC Filter FAM Result OK Filter ROX Result OUT Positive Control 5 ROX Sample Name L 16S PC Filter FAM Result OUT
12. http www takara bio co jp 15 CY214 Takaka Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp STII 7A ARS 4 v201401Da
13. PCR 2 SE raat 3 3 1 2 3 http www takara bio coJp 4
14. CY214 V V 1 DNA 2 EXTRAGEN Il DNA EXTRAGEN Il 1 0 5 ml 5 000 rom 5 2 100 ul 8 ul 1 1 5 ml 10 12 000 rom 3 2 2 500 10 PTET 4 4 12 000 rpm 3 3 2 200 ul 10 fit IT So 6 12 000 rpm 3 7 RNase free dH20 40 DNA RNA
15. DNA Ik 1 5 uu 1 ul 25 ul 2 DNA Positive Control 2 http www takara bio co jp 5 CY214 2 DNA 3 DNA Positive Control 1 pl 0 2 ml PCR x Probe Primer Mix 5 6 2 Positive Control Positive Control FAM Positive Control ROX 2 1 3 PCR 3 Therm
16. http www takara bio co jp 7 CY214 Plate Image Filter FAM FAM Target Filter ROX ROX Target 1 Plate Image Plate Image Editor Target 2 Plate Image Plate Image Editor Sample Target amp smo Seting Plate image Prehadenmar E Pobune b Piebe re Poche rri PaebaPrinard a iain DS Perobal rira amp haFrre O p Some Un ot ancrer 2 Ines O tom OO Ji Ne ET d 3 URRH 4 FE 5 Po 6 Po 7 Re i ee Omit http www takara bio coJp 8 CY214 5 Result Analysis NC PC 5 1 NC Selector N gt Filter FAM Filter ROX oe Be ao 3 Ae Purra Em o Eheee Pamay Tuna eeSBBeseBaeS SE i ee ee ek ee re ee ee ee ee ee Crt RB PUR MRNM EMRE Thi Cech Filter FAM
17. Filter ROX Result OK Detected F Primary Curve Final Filter Detected CP Primary Curve Ct Filter Detected SDM 2nd Derivative Ct Filter P M Result F Primary Curve Final P M Result CP Primary Curve Ct P M Result SDM 2nd Derivative Ct http www takara bio coJp 10 CY214 V 3 40 PCR Thermal Cycler Dice Real Time System Result Analysis Analysis Data Text Report 2nd Derivative Ct Ct SDM Ct
18. Start Run 4 Plate Setup Target amp Sample Setting Target List Sample List 4 1 Target List Dye Dye of Internal Control none Target List Probe Primer Mix 1 Add Target List Delete 2 Dye 3 Name Target Name 4 Color 4 2 Sample List NC PC 1 Type Type UNKN Unknown PC Positive Control NC Negative Control 2 Name Sample Name 3 Color 4 3 Target List Sample List Update Target amp Barrel Efing Pat image CHi Mimar eer TE HC E z TETTEIT sinl TERETERE
19. ul 10 8 dH20 750 ul 9 Positive Control 1 FAM 10 ul 10 Positive Control 2 FAM 10 ul 11 Positive Control 3 FAM 10 ul 12 Positive Control 4 FAM 10 ul 13 Positive Control 5 FAM 10 ul 14 Positive Control 5 ROX 10 ul 15 Positive Control 6 FAM 10 ul 16 Positive Control 6 ROX 10 ul x1 dNTP Mixture vortex K2 FAM ROX RRT IPRA m ie pees Streptococcus pneumoniae ane l ves gt KJ m ie RU Haemophilus influenzae i z ON Mycoplasma pneumoniae 1 ONARIR ZIRT 7 ee ae Probe Primer Mix 4 Chiomudopniepheumonee 16S rRNA ao i fi Na ee hi 00 2 2 Probe Primer Mix 5 Segre Doamna 16S rRNA A ey ne e a KD Probe Primer Mix 6 SHED iotocc Is pyogenes 16S rRNA SLO 1 Haemophilus influenzae 16S rRNA
20. al Cycler Dice Real Time System Thermal Cycler Dice Real Time System Software Thermal Cycler Dice Real Time System 1 Run File New Experiment Options Experiment Type lt PM M Plus Minus Assay Multiplex gt gt Hew Experiment Options Experiment Type Select Experiment Select Single l Multiples sas OE E Single solute Quantitication C AG Multiples C Ras sida Guanine Single elate Ghantitication Re Multiples Singl Plus Minus Assay HEL Multiples SMP Genotyping Assay Multiples 2 Thermal Profile Setup PCR Probe Primer Mix 5 Probe Primer Mix 6 2 FAM ROX Collect Data FAM ROX Hold Cycle 1 95 C 10sec 3 step PCR Hold Cycle 40 95 C 5 sec 55 C 15 sec 72 C 20sec collect data al F F 7 i Terrace there deal http www takara bio co jp 6 CY214 Start Run
21. ip tube individual Flat Caps NJ600 X Cycleavee TaKaRa Ex Taq Thermal Cycler Dice Epoch Biosciences Inc
22. ny research or services where the results of any test or assay are used for patient management care or otherwise in making therapeutic or treatment deci sions for a patient A portion of this product is subject to proprietary rights of Epoch Biosciences Inc and are made and sold under license from Epoch Biosciences Inc under the patents and patent applications US20020155484 WO0142505 WO02099141 US10 113 445 US09 876 830 and corresponding pat ents issued in other countries There is no implied license for commercial use with respect to this product A license must be obtained directly from Epoch Biosciences Inc with respect to any proposed commercial use of this product and commercial use includes but is not limited to A the sale lease license or other transfer of this product or any material derived or produced from it B the sale lease license or other grant of rights to use this product or any material derived or produced from it and C the sale lease license or other transfer of kits which include this product 0 NANOGEN M40 Thermostable RNase H This product is covered by the claims of U S Patent No 7 422 888 and its foreign counterpart patent claims M46 PCR Cycleave This product is covered by the claims of Japanese Patent No 4022522 M57 LA Technology This product is covered by the claims 6 16 outside the U S corresponding to the expired U S Patent No 5 436 149
23. s hey idee kaoi a k Penne Wek Creel Sect beeen se T AT BY BY Cr Cr D7 Dr Er Spyo NTE Spyo NTE Spyo Spyo 4 Spyo B Spyo B Spro Spyo Spyo G Spyo G Piria Pray eel Se ote eeves goa g e g a ee a a i ee ee o arr nyn 165 Gnwn SLO Spyo 165 Spyvo SLO Spyo 165 Spyvo SLO Spyo 165 Spyvo GL Spyo 165 Spyvo SLO ITLESTEY LIST il Seats tevea alge e 2 A B C G http www takara bio co jp 12 CY214 Vil 1 1 DNA gt 2 PCR Positive Control GE PCR 2 Thermal Cycler Dice Real Time System primary curve
24. t Start PCR ASR TaKaRa Ex ee HS RNA DNA RNase H oe ee RNase H RNA 1
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