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1. DNA PCR PCR 0 PCR DNA KAPA3G Plant DNA 2 5U Plant PCR xX Plant PER 1Q0 MgCl2 G5mM e Y gt gt DNA PCR 45 FastPCR AA 2 http www n genetics com 113 0033 6 17 9 3F Tel2. 96well 384well GPRITM 40 1 96 15ul 3G384 4C 6 COUDER 800 preps 68 000 5 000 preps 340 000 86 000 KAPABIOSYSTEAMS 2 Kiieemtemt5l 1 2 0 KK7150MG 2 KAPA IO 4500 KK7154 KAPA 500 112 500 2012 4 1 6 0 DNA F2XPCH 120 KAPA Express A TS EN TS KAPA2G Robust HotStart ReadyMix PCR 0C 6 1 75C 10min
3. FastGenerw 5 5 EY Midori Green Advance 50 50u 2 s 300mg POR 100l D R FE CR lt 300mg se FOR GP1 Midori Green Advance DNA Stain MR 1 a 6 Cat No NE MG03 3 50 1 500 NE MGO4 1ml 19 800 NE MGO4 5 5x1ml 70 000 M 0 a D T BM 30sec 6 30sec TF a e600ul GP e000ul GP 13 000rpm 13 000rpm 30secC 30seC ss TBE 2 s 2 5 2
4. 100ml 25 0 5cm g 1 4C Ce 13 000rpm min 20 50ul GPS 6min 13 000rpm min 13 000rpm Smin 0 50ul GP3 min 13 000rpm min BUUrms bms US MG Advance MG Advance UV light UV light LED illuminator 1 Midori Green DNA Stain dsDNA SSDNA HNA 490nm 530nm Secondary Peak ait 4 PCR DNA 10 PCR DNA ee s DNA HyperLadderV BIO 33031 10 e 20 10 e 496 TAE 100V 30 C ont DNAg RS
5. 50 1 0 796 TAE 100V 20 1 6 7 6 0kb 25 8 EUU 4 5K6 0 LU CU849 TARA DNA SNsNWeIeNlse 0 2Sa DNA e BigDye Terminator v3 1 130 140 150 CGCGCTTGGCGTAATCATGGTCATAGCTGTTT el 0 220 230 240 250 260 GCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAAT MW MM TGC es 22 DNCWMI Mo 1 s ABI3100 POP6 FastGene RW 3 FastGene CAGCTG E
6. 1 PCR PCR 62 3mm 95C 5min 68 000rpm 1min 2 PCR 100u 20C 3 KAPA Express Extract Buffer 10x 10 KAPA Express Extraot 1U 2 88 2 KAPAPGRobust HotStart HeadyMix x 9 10mM Tris pH8 0 8 5 TE 5 10 New PCR 0 KAPA3G Plant PCR Kits Kit size KK7251 250 50 I 50 000 KK7252 500 50 I 90 000 2012 4 1 6 30 PCR TR KAPA3G DNA 3 PCR
7. mP1 25ml 65ml AI mP2 25m 75m Ah 2 NAM 6 s mP3 40ml 100ml 26 36 1 mP4 50ml 130ml e 2 mP5 25ml 40ml s mP6 10mMTris Cl pH8 5 10ml 30ml 15k NO 2 LB 10 25 1 3ml gt 10 000rpm Tmin 500 mP1 500 mP min 300 mP3 13 000rpm min 13 000rpm 30sec 150ul mP4 300ul mP5 1 13 000rpm 3min 50ul mP6 Cmin 13 000rpm min 0 4 1 5ml gt 10 000rpm min 00 mP1 500 mP min 300 mP3 13 000rpm min 13 000rpm 30sec 13 000rpm 30sec e600ul mP5 13 000rpm jao9 pn 90secC 13 000rpm min 50ul mP6 min 13 000rpm 5 10ml gt 10 000rpm min NN 400gul mP1
8. 320 330 CE CN CC CRE je CRE CTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGC MM olly ul DNA GGCGAG lt os co D lt he FastG a DN A PCR a ee ene Gel PCR Cat No MI FG 91202 FastGene Gel POR Extraction 100preps 9 300 FG 91302 FastGene Gel POR Extraction 300preps 19 200 FG 830 2 Om lt BI 50 5 000 PCR DINA 5 FG 91202 FG 91302 20 RR e FastGene GP 100 300 10u8 gt 2 ml 100 300 NM SOD DN TDKNR GP1 80ml 200ml 2 GP2 25ml 40ml 207 s GP3 10mMTris Cl pH8 5 10ml 30ml
9. 100Dbp PCR 50Dp DNA 50bp 100bp DNA GP1 PCR DNA DNA GP1 GP1 DNA gt GP1 DNA 1 DNA 500 400 300 250 200 175 150 125 100 75 50 25bp 2 Binding buffer GP1 3 11 Binding buffer GP1
10. 6 oy WAM 0 120 130 140 150 160 GGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCG TAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG1 750xg BD Mi 3min 50 580 399 409 4 229 OO fi reececrrsecerarrecececrcrmccec rrCCrGCcAGeACrCGCTeCGcwcGe TCPeCGGCTecGGCeAeCGGTAwCAGC CACTCAAAGGCGG TAM MM WevAVsa22 2 0 FastGene X s 20 2 5 BigDye Terminator v3 1 ABI3100 POP6 ne 1 2 288 2 00U000 i i es 1 96 384 PCR KM a BC A63880 Agencourt AMPure XP Kit 139 278
11. 400ul mP omin 600ul mP3 13 000rpm 3min 13 000rpm 750 X 30seC 400 mP4 13 000rpm 30seC 600 mP5 13 000rpm 30seC Cs 0C 0 13 000rpm min 4 5Oul mP6 70C Cmin cc min 13 000rpm min f DA J J 1 2 PBSK DHS5 FG Q s DNA e e e M P 42 7 40 2 26 4 12 2 SN DS LB Ampicillin 1 1 5ml 50 5 0 796 TAE 100V 20 2 a a M 1 So 3 4 5 6 7 8 C M PCR TOP10 1 1 5ml 8 TOP10
12. 10prep FastGene W DT 50 20l e FastGene G50 5 X525 mg 525mg 10prep 5 UE 2 50 1 4C 14 DT ON DNA FastGene CR TATAG GGCGAATTC GAGC TCGG TACCCGGGG ATCCTCTAGAGTCGACCTGCAGGCATGCAAGC CR RR id 8 0ml DT gt 30min AvWW0 My MM MM a 3min M 750 4 M 10 40 50 60 70 80 90 100 1 ITGTATC GAC N TAT GC TCGG CCCGGGG ATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGAGTATTCTATAGTGTCACCTAAATAGCT
13. DNA 30 AMPure XP Q E DNA ng ul 200bp 800bp DNA AMPure XP 00 800 10 000bp PCR AMPure XP DNA 10kp Clean SEQ PCR OOUDD DNA ng ul DNA DNA DNA DNA 00 800 10 000bp DNA DNA AMPure XP DNA Cat No BC A29151 Cat No AGO00121 8ml BC A29154 HCatINo _AGOO0136 50ml BC A32782 SPRI 96 Super Magnet Plate
14. 03 3813 0961 Fax 03 3813 0962 604 8277 565 3F Tel 075 257 5421 Fax 075 257 5422 10N1203D30K
15. 2 E5 24 FG FastGene Gel POR Q Q M M P P FastGene Gel PCR 25bp DNA 50bp DNA 25bp Q P 25bp DNA M F8 Et 100bp PCR 10 TBE se sh 3 M 1 2 3 4 5 6 s TBE 100D p PCR e 20 1 10 e 2 096 TAE 100V 20 FastGene PCR M
16. 496 80min DNA 960ng 5 1 10 1 Binding Duffer GP1 1 1 11 2G0 1 GP1 1 2 AU DNA 7 Agilent Bioanalyzer L Lower Marker 11 10uL U Upper Marker 173 FU 0 200 100 0 Lk kJ An 15 100 200 300 500 700 bp 15 100 200 300 500 700 bp 15 100 200 300 500 700 bp 4 Fu SE OS Cat No FG 9411 FastGene Dye Terminator Removal 50prepS 7 300 1
17. 5ml 24 000 BC A63881 Agencourt AMPure XP Kit 1 666 3 333 60ml 98 000 BC A63882 Agencourt AMPure XP Kit 12 500 25 000 450ml 498 000 BC A32782 SPRI 96 Super Magnet Plate 86 000 dNTP PCH 100 DNA Biomek ODNA 96wll 384well BC A32782 SGPRITM 2 UI OO OO SU KM 4C 18 AMPure XP 100bp DNA DNA
18. RAIA NIPPON GREE Geneticscoria Ft ene ab 4 a FastGene Plasmid Mini y LB preD ap a _ PCR FastGene Gel PCR Extraction v6 4 prep astGener JDye Terminator Removal NA ME NG DNA Cat No FG 90402 FastGene Plasmid Mini 100preps 10 500 FG 90502 FastGene Plasmid Mini 300preps 21 500 NE L2720 100 LB 100 7 100 Fast FG 90402 FG 90502 e S DE IS 5 0 3 a SS 0 e FastGene M mP 100 300 7 RN 2 ml 100 300 a ST
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