VP-DSC Manual（Japanese）
Contents
1. GE 2SSN M 169 0073 A 3 25 es SN TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com2. 60 9 4
3. 4 Feed gock Mode Gain mid Stor Experimental Parameters Number of Scans Post Cycle Thermostat C J Cell Concentration mM Scan Parameters Starting Temperature C J Final Temperature CJ ScanRate C Hr PreScan Thermostat min lt PostScan Thermostat min Filtering Period Sec 5 Origin DSC 6 Tools Pick Peaks Find Peaks 7 28 2 2 C 75 9 2 C 34 6 Syringe Hamilton 2 5 ml 4 375 20GA SYN161709 Filling Tube Acrylic Cellport Funnel Assembry O ring Viton 016 Pack of 10 I D x
4. AT1 2 AT1 AT1 DP Jacket Feedback Sample Cell Feedback MicroCal VP DSC 2 2 2 MicroCal VP DSC System MicroCal VP DSC
5. 1 130C 90C hr 0 30 psi 80 psi Origin T J AH ACJ 2 3 Raw Data Raw Data Origin Tm AH AC RNasedsc_cp bufferdsc_ cp 0 00014 0 00013 0 00012 0 00011 0 00010 0 00009 Cp cal C
6. 2 2 1 DSC DSC DSC 2 Tantaloy61 500 ul
7. 5 5 1 VPVjewer2000 Setup Maintenance Pre Run Data SC Cann T irra moat nlibi IE Td Caia File Path CWP Sian elup Flm Fialh IF WWFHISLtmEHump Im mlugFlm Ladanl son Cells BuglTmmm WE Hh Rmmp Time lser I mamln lng rrr st 5 Pranlts nemnl se eftt ll mm lm Idle ata 2
8. Tm AH 100 Iter NonLinear Curve Fitting Fitting Sessinn Category Function a amp ction Options Scripts Parameter Yalue Way Error Dependency Tm le1 54 iv In 358 In134 H 1 062E5 I 1871 In1134 Fress Esc key to stop fitting iterations Chi Sqr 1lteW TOD Tter Done Enter fitting session anpererfi nonlinear curve fitting Basic Mode Reduced Chi sgr Chi sgr js not reduced Done 25 NnnLinear Curve Fitting Fitting Session Category Function A amp ction Options Scripts Parameter Value Wary Error Tm l1 55 iv Dependency no 355 In 135 H 1 062E5 186 9 no135 Reduced Chi sqr 110025 89779 Total 4 rounds in this session 1 Chi sqr is not reduced Reduced Chi sqr 110025 89779 Total 4 rounds in this session Perform a specified number of Levenberg Marquardt Basic Mode terations Press ES to stop 6 ha HzEH
9. 7 Dpone init with fixed Tm s OK 100 rer Reduced Chi sqgr Chi sqgr is not reduceg pone 10 27 2 1 P 20 4 1 P 21 4 3 2 P 22 4 4 1 2 3 Adjust Move Segments by Cursor
10. NMR 110 240 VAC
11. 3 4 5 1 Orjg 7 0 Real Time Plot Display Mode Rescale To Show All Display Mode Completed Scans Rescale To Show ll 2 X lt Y Scg e From To OK Minar Tick Labels Title amp Farmat 16 3 pjsp gy Mode Comp ered Scans Aeg Time Plot Display Mode Display Mode Real Tirne Flot Completed Sa Rescals To Show Rescale To Show ll DF Scale Cortroals 3 4 6 1 Stop VPYiewer2000 Default User System DSC Help BB Load Run File Save Run File
12. gnter Origin 7 C Origin7OWNHTITLED CE i aseline Cancel Window RFit LgFt segment Refit Right Segqment ylnwE 5E TBL5 by UF5 T Cp kcalmole C 4 Baseline Step at Peak rEn OriEn TEUNTITLED HormDAT EE dst WEE ahcEl Wndowm Froegress Hasgling Linear Kornnect Cbic Fone k Sten at Peak howe Baseline hy Dursnr 28 Native Denature Cp kcalfmolef C OK Subtract baseline No Data Reader OriEm 7 C Oriemn70 UNTITLED NormDATA OK Adjust Baseline Cancel Window 15 Data Reader Y Data Reader
13. 31 reed Back Mode Gain Mone VPVjewer2000 DSC Controls SIgr Experimental Parameters Number of Scans lt Post Cycle Thermostat J Cell Concentration mMl 0 Scan Parameters Starting Temperature C J Final Temperature J 110 ScanRate C Hr lt lo PreScan Thermostat min PostScan Thermostat minl WN Filtering Period sec 32 5 6 1 1 2 Feed Back Mode Gain None DSC Controls Experimental Parameters Number of Scans lt Post Cycle Thermostat J Cell Concentration mM 0 Scan Parameters Starting Temperature C J Final Temperature 110 ScanRate C Hr 0 PreScan Thermostat min PostScan Thermostat min 0
14. 1 ThermoVac 3 4 2 ThermoVac 3 10 4 vacuum 0 5 3 5 6 vacuum TEMPERAT lt ons 3 ThermoVac am 3 3 1
15. 2 5 5 lt lt 3 MicroCal VP DSC Test Kit DSC RNase 2 CMP KIT100010 1 2 RNose Tm AH 3 3 1 1 PC
16. a O O 4 CN
17. 9 00 17 30 TEL 03 5331 9315 FAX 03 5331 9349 TEL 03 5331 9336 MicroCoal FAX 03 5331 9370 e mail Tech JP ge com O amp A Science Program Web 6000 Www gelitesciences co jp 2012 GE
18. 2 30 3 20 Contrad 70 Decon90 4 VPVjewer2000 Main Window Thermostat Calib 5 Thermostat Confro 70C Set Point QQ 70 Set Jacket Temp DSsC Coatrols cr 7 Maintenamce Constants shat Conkie Set PF se 30 6 70C 1 7 1 Thermostat Control 25C Thermostat nnirnl et Pont CU 8 20 Contrad 70 Decon90 9 5 10 11 20 Contrad 70 Decon90 lt
19. 2 5 4 1 2 1 2 1 2 1 2 3
20. MicroCal VP DSC 1 130 C 10 110 2 5 3 MicroCal VP DSC 90C hr 60 90C h High High High Mid DNA RNA Low None
21. Contrad 70 Decon Laboratories 3 6 1 VPVjewer2000 Main Window Sysrem Quit Progrom 2 VP DSC Off 3 PC 4 UPS Off On On 19 4
22. AC Cp kealimole C 5 MicroCal VP DSC 8J9 5 1 P 18 3 5 5 2 5 3 lt 2 3 1 5 4 1 50C
23. 2 lt 2 3 7 8 DE 11 9 3 8
24. RNAdse 7 4 Tm 4H AHv 4rv 4H 2 2 Z4 9 DSC Aon 2 Srgre Cursor Init Dripin 7 C MOrigin7O0MUNTITLED RNASEDSC_CP File Edit View Graph Data Math Peak Tools Farmat Window Help Set IndenJ5enu Model Indep 2 5 ate Cursor Init 2 State No Init 2 State i Man 2 State From 2 State Man 2 State Simulae 2 State widCp Cursor Init 2 State widCp No Init 2 State widCp Simulate Dissociation wdCp Cursor Init 1 _cancel Cancel Number of peaks DD 7 Dpone init with fixed Tm s OK 9
25. 10 27 psi 50C ii 2 12 3 4 RNase 3 4 1 1 Apply Eomments to all VPVjewer2000 Main Window DSC Controls Scan Edit Mode dentical Scans
26. hm Pe OFF TEL 03 5331 9336 ED
27. 3 9 8 No 4 5 Origin 4 1 2State 2 Tm AH 2 Non2 State 2 Tm AH AHv 3 2State w dCp 2 AC Tm AH 4C 4 Dissoc w dCp Tm AH 4Cp n Tm AH AHv 4Cp n Ao 2 State
28. Origin 7 C Origin7OWHTITLED CE i Easeline Cancel Window 1 RFit LgFt segment RaFit Riqht Seqment ne Seqments hw ursnr Baseline Progress 8ose jne Linear Connect Cubic Connect 5Len at Pa tsp at Half rga oe Basgline by Cursnr Cp kcalmole C Tempergture C Baseline Move Baseline by Cursor Li Origin C MOrigin7OMUNTIILED RNAS OK adiust EE Cancel Window Progress Baseline a Linear Connect TP Cubic Connect Step at Peak amp Step at Half Area lt lt mm Mawe Baseline by Cursor gt OK Subtract baseline Yes AC 2 State w dCp Dissoc w dCp Ao
29. DSC Controls Conventional DSC Controls Experimental Parameters Scan Parameters Number of Scans 20 Starting Temperature C uaAUL oo ME mw PreScan Thermostat min Cell Refill Parameters EC EE 1 Use Audible Fill Indicator PostScan Thermostat min Filtering Period sec _ Fill Bet d Between N an N A Auto File Name scanmldsc DataFile Comments a None Low Mid Scan Edit Mode ldentical Scans Unique Scans Experimental Parameters Number of Scans Post Cycle Thermostat Q Ce Concentration mM 9 Scan Parameters Starting Temperature CJ Final Temperature CJ ronNee 9 0 Postscan Thermostat min 0 13 reed Back Mode Gain Low File Name Setup Maintenance Data File Path
30. MicroCal VP DSC 2 VPViewer2000 3 MjcroCo VP DSC 4 VPViewer2000 Main Window Origin7 0 Real Time Plot 2 3 2 ThermoVac ThermoVac
31. RNose DSC 1 Origin DSC Microcal LLC DSC LE i Himlmf 1 LLG SG 2 DSC Main Control Read Data dsc lt Agd rje s OK ReadData_ Head Data subitract HefErenre re EE pase Hormalize Concentration oy Ma m mm Po Fie pame RNase dsc Asariets Files of type pscoa al dscl lt Hemove Fiets Carcel Fle Name 3 jn Eripin POINTITLED Here non 1 mm018 1 Int1 1 Ly se a ONO oe i 1 Me_ ii naarT LIU 20 4 2
32. mn Model EE HImThoF 1T1 TT 1 I LH 1IE1E 1 L4H 1 1 BE Ep kcalmnlg E1 Th Temperatm rs File Export Page PDF TIF 26 4 6 2C 4C 2 1 2 1 2 1 P 20 4 1 P 21 4 3 P 22 4 4 1 8 Subtract Re7erence No DSC 2 Srgre w dCP Cursor Init
33. Unique Scgns Even Ogg Start VPViewer2000 Default User System DSC Help Ll gt I Load Run File Save Run File Start Compact Mode 1 Scan Parameters 2 Scgn Edit Mode Unique Scans Scan Edit Mode dentical Scans 3 Update Run Parameters VPViewer2000 Default User System DSsC Help Laad Run File Save Run File Display Run PararN Update Run Param Stop Compact Mode 4 Display Run Paramet
34. 1 DSC Main Control Subtract Re7erence Subtract HEfErErmrg _ Mnrmalize Concentration Subtract Reference Data Dogrg Re7erence OK Subtract ReferenceData Pa bataFelerence asENDSCE CC Reterence bufferdsc cp es Lancgl RMagedgc ch Eprca 1 EE BB Oi Om Th h Tem pe te 4 3 cal kcal mol 1 DSC Main Control Normalize Concentration Subtract Relerence l Mnrmalize Concentration BP Concentration mM mM OK
35. Cell Vol ml caldeq gt cali deg M for RNasedsc cp Cancel Concentration mM 0 06 Cell Vol ml 0 507 cal C kcal mol C RhHazedgc Ep rikcalrmnls Tempenatire 1 4 4 1 Peak Start Baseline Sessjon Drigin CMOrigin70WUNTITLED NormDATA File Edit View Graph Data Math EN D ae kl kl Ll _ 5 Cursnr Draw Baseline adjust Easeline Cursor Pick Baseline Integrate From 0 Integrate From Easeline Cp kcalmole C 40 Sn 5 TD gD dn Temperature C 2 Adjust Move Segments by Cursor
36. pH pH 2 4 2 DMSO 2 4 3 DTT B TCEP 2 DTT 2 5 VP DSC MicroCal VP DSC 1 700 700 ul 2 5 1 MicroCal VP DSC 1 mg ml 0 02 mg ml
37. Dgzg File Path ser Setup Data File Path ICE ywPDSFtdaka Setun File Palh CWPDS Csetup IniL Setup File lLastunl sen Cells Boot Temp FI Hitr Hamn Time 5 sec na naln Input range r altsl Current User Default User ae T Ans Scale 8 Plnt Idle Data mCal min Erpnrt Eurrent Data uCalfsec 1 Pre Run Data lt pfatt Data Fath X Ec Svstem Fnlder Hame Itesl Add Folder Feeg Back Moge Gog DP High Mid Low None 4 High High Feeg8ockMoge Filtering Perjod FeedBackMode 4 1 3 Filtering Period 14 3 4 2 3 4 3 FeedBackMode Mid Low Aone Sample Scgn Edit Mo9e ldentical Scgns
38. Filtering Period sec 3 Start 4 DP Thermostat Calib Pu se Control Setup pu se Sjze 5 Duration 300 Pulse On 5 1 1 33 5 7 1 1 1 VPViewer2000 2 3
39. 0 00008 Cp kcalimolerC 0 00007 0 00006 Temperature C Temperature C 2 4 MicroCal VP DSC DSC 2 4 1 pH lt Tris Good
40. Display Run Param Lndate Run Param Stop Compact Node 17 3 5 1 3 P 11 3 3 50C lt 2 20 Contrad 70 Decon90 600 ml 3
41. O D 15 9 x 19 1 mm Test Kit DSC RNase 2 CMP 1 ml 1 ml 0 35 MSP12021 MSA12030 ORGO0016V 010 KIT100010 GE Healthcare mW NN FO HH DR OD
42. 30 SCOMD FTF RI NN NU 30 LM Lurene 30 SFI 31 6 c 4 33 1 0 34 6 ee 35 1 MicroCal VP DSC lt p MicroCal VP DSC 100 cm PC MicroCal VP DSC 70 cm ThermoVac 25 cm
43. 4 O 1 VP DSC 2 ThermoVvac 3 4 gt x 4 amp 4 5 20 Contrad 70 ThermoVac 5 ThermoVvac 6 20 Contrad 70 Decon90 300 ml 300 ml 7 8 3 lt 7 18
44. GE Heqlthcare Life Sciences MicroCal VP DSC GE imadination at work 1 3548 31 2 4 LMM uucuuiiiisaateeeeeeeeeneeeeeeeeeeeeeeee 7 2002 EE 2 4 2 MMMM 2 5 VP DSC kL 3 2 5 1 2 5 2 2 5 3 NE 2 2 5 4 2 5 5 9 00 I 10 3 2 ThermoVac MMMM 10 3 3 11 RINGSS 2 EE Eo 13 13 SEE 15 3 4 3 ee 15 3 4 4 H A ao uaauaa saatssuuuussssseeususseuiseesseeneeseecereeseeseiesseicnes 16 3 4 5 Re Gi 16 3 4 6 17 255 Oo 18 NN TIN SA 19 a 20 4 1 ee 20 2 E18 Se 21 21 NO 22 EF TI A 24 4 6 ACp LL i 27 30 So 30 EE 30 53p oo 95883Re
45. ers 15 3 4 4 1 2 15 25C 3 P 11 3 3 4 50C lt
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