TPMT＊3Cのジェノタイプ検出 −SNP Typing Kitの使用経験−
Contents
1. TPMT TPMT 3C kit DNA I DNA SNP Typing Kit Thiopurine methyltrans ferase 3C 719A G PCR Cycler BioRad _ sa PCR DNA PCH PCR UpIo3d Go 1 95C 30 sec 65C 930 sec 72C 30 sec 1 72C 5 min2. PCR F Hoffmann La Roche PCR 40 000 UpIo3d
3. A specific Primer G specific Primer M 1 2 3 4 5 6 7 8 9 10 603bp Lane M Haell digest Lane 1 6 negative control Water Lane 2 7 TPMT 1 1 Lane 3 8 TPMT 3C 3C Lane 4 5 9 10 Patient DNA 1 ASP PCR detection for TPMT GC MM GAAAAGI TATATCTACTTACAG Electropherogram sense strands for the TPMT gene at exon 10 Wild Wvild A719A Seqguence was determined using ABI PRISM 310 Genetic Analyzer 1 Krynetski EY et g Pharmacology 61 136 146 2000 2 Hiratsuka M et al Biol Pharm Bull 23 1090 1093 2000 3 Hiratsuka M et al Mutat Res 448 91 95 2000 4 Ando M er al Pharmacogenetics 1 1 269 273 2001 20C SNP Typing Kit Thiopurine methylItransferase 3C 719 ATG 200 STK 151 40 000 SNP Typing Kit N Acetyltransferase SNP Typing Kit N AcetylItransferase 2 6 SNP Typing Kit N Acetyltransferase 481 C T 590 GA 857 G A G0C CodeNo 2004 2005 S00 STK 131 40 000 S00 STK 132 40 000 SNP Typing Kit Glutathion S transferase A1 69 C T 00 STK 14 1 40 000 SNP Typing Kit Alcohol dehydrogenase 47Arg HiS STK 161 S00 STK 133 40 000 5 40 000 SNP Typing Kit Aldehyde dehydrogenase 487G A
4. STK 162 40 000 SNP Typing Kit Cytochrome P450 2850 C T STK 1 1 40 000 SNP Typing Kit Cytochrome P450 0D6 4 1846 GA ST K 1 40 000 SNP Typing Kit Cytochrome P450 6 whole deletion STK 123 40 000 SNP Typing Kit Cytochrome P450 6 7 100 C T 00 S1K 1 4 40 000 4 4 SNP Typing Kit Cytochrome P450 6 7 1758 GA 00 STK 1 5 40 000 4 47 SNP Typing Kit Cytochrome P450 7 6573 C insert 00 STK 126 40 000 4 47 SNP Typing Kit Cytochrome P450 0D6 S36 Exon9 2D6 2D7 SNP Typing Kit Cytochrome P450 0D6 Duplicate 2D6 x2 S00 ST K 1 STK 128 40 000 40 000 4 47 SNP Typing Kit Cytochrome P450 2 2C9 3 1075 A C STK 101 40 000 SNP Typing Kit Cytochrome P450 2 79 681 G A STK 111 40 000 SNP Typing Kit Cytochrome P450 2C19 3 636 G A Positive Control Set 12 STK 1 1 20 000 Code No Code No P PCR Polymerase Chain Reaction F Hoffmann La Roche 4 683 195 4 683 202
5. min a 4 1 1 TPMT GC PCR 608 bp Fig 1 A G G SNP Typing Kit TPMT 3C kit
6. ECHNICAL EVIEVV TPMT 3 SNP Typing Kit 6 6MP Thiopurine S methyltransferase TPMT PhaseI 10 TPMT SC TPMT 6 9 1 TPMT 3C 6MP FDA 6MP TPMT TPMT 6MP
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