BIOSCIENCE & BIOTECHNOLOGY
Contents
1. 6 1 6 2 6 6 6 5 EE 1 1 lt 6 62. 5 2 09 PVDF 5 2 0 1 Towb in 100mM 25mM 192mM 5 20
3. A mr FN 1 2WLR 4M RI WSE 4020 WSE 4040 205x100mm 205x200mm HC 2I R 4M R
4. 2 4 6 3 6 3
5. 0 0 1 0 029 SDS 9 0 CB B CBB
6. 6 5 C 2 mA cm2 144mA 50V 30 40 6 2 6 1 6 3 Towb in 6
7. AE 1460 EzBlot A 0 3M 5 a B 25mM 5 C 25mM C 3 40mM 6 5 PVDF B 144mA 2 nmA cm2 B 1 25 35v 30 A 2 WSE 4020 2M R WSE 3500 HC A lt C 100 ml 1 2 1 PVDEF PVDF B 3 0
8. 1 10 lt 20 30 i 2 POD HRP X CCD CCD Aop
9. PVDF 3 10 209 6 83 3 PVDF PVDF 6 4 1 2mA cm2 30 lt 9 0
10. 6 3 2 0 2 5 1l Kyhse Andersen J 1984 J Biochem Biophys Methods 10 203 209 6 2 2 2 0 10 lt 5
11. Southern Towb in 1984 Kyhse Andersen Kyhse Andersen J 1984 Biochem Biophys Me thods 10 203 209
12. WSE 6200H _ a 8 InonrRI AE 6530P WSE 3200 E 520L SDS PAGE SDS PAGE 0 e AE 1430 EzApply AE 1410 EzRun ni Pe 13 800 6 800 4 800 WSE 4020 WSE 4040 WSE 3500 3 AE 1460 AE 1465 RE 2MHR 4MR pgzgp7 ExzFastBlot atto HC 12 000 9
13. AE 1476 ExzBlockBSA BSA B SA AE 1477 EzBlockCAS CAS WSE 7230 ETBS WSE 7235 EzTween 1 HRP lgG Xx1 1000 TBS AE 1490 EzWestBlue TMB AE 1495 EzWestLumipluse 1 Tween 20 2 Alp 3 3 7 5 2 lt 2 Pd 1 2
14. HIC WSE 3500 3A 150V PVDF polyvinyliden difluoride P PVDE PVDF 2 4 P
15. URL http ww atto to jp
16. 0 001 CBB 10 309 2 10 509 1 C B B 1 10 1 100 2 5 0 109 309 100mnL 45mL 52 CBB 1 5 10 1 _ lt a 0 5 2 5 AE 1475 EzBlockChemi
17. PVDF 1 2 10 0 05 Tween 20 Triton X E NN MN P PVDF Miki ET 0 9m
18. 000 98 000 BS HRP HRP AE 1475 ExzBlockChemi WSE 230 EzTBS AE 1490 EWestBlue WSE 120 WMestumipse WSE 6200H LuminoGraph I 9 000 5 000 12 000 22 000 I 2015 5 NNA 111 0041 3 2 2 h815897 4BH1 31589 6647 0315827 4873 03 582 4874 1 10 QU1B 58 H D8 518 BH D 5818 530 0044 2 8 1 0EJ8135 1421 0B 56 3625
19. 4 I BIOSCIENCE amp BIOTECHNOLOGY 51 SupportingLifeResearch 1 Western Blotting Ri 1 9 7 9 DNA
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