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タンパク質精製の溶離液の濃度勾配、切換えに

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1. 4 LPG 1000 4 0 1 3 0mL min 4
2. LPG 1000 FILE NO No 165050 STEP TIME 1 Wait Tn 3 1 U 0 1 lt 3 0mL min Al12 13 MP 1000 02 20 RE 0 5 5 0mL min VSP 2050 03 20 0 1 1 9 1 30 999 9min Step Ue ATMB TT C 1 Im 1 99 Wait 00 0 100 0 0 Link and Loo0p 1 9 5 15o 1 8601 26 Oo 1 1 9 1 99 0 Me
3. 1mL min 4 gt SE S a 700 2 4 0 1 3 0mL min HPLC 1mL min 4 FT LPG 1000 DN 0 5 5 0mL min 48mL min BG 34 10 LPG 1000 _ VSP 2050 VSP 2050 10
4. AC 2 400VA 0 START STOP 1 05 15 0 40 40 20 START END 4 06 25 0 20 50 30 1 5mL SUS 4 MEEB 100 1200rpm 4 x 10mm 08 30 0 0O 50 50 6 8 5 35 C 188WxX330Dx173H 4 8kg mm 122Wx250D X195H 2 5kg 1A 0 5A AC100V 50 60Hz 480 000 MP 2000 MP 3000 VSP 2050 30 Al 12 13 KP 22 13 min Link and Loop File Link and Loop File 1 1 1 Link and Loop File Mo 1l File No Repeat 2P422 EP419 EP 530
5. 48mL min 1mL min 4 an 500 L LPG 01M LPG 50M No 180800 180790 Al 12 13 KP 22 13 VSP 2050 MP 2000 3000 1mL min 10 48mL min 130L 10mL 99 000 150 000 0120 076 554 EYELA ki
6. For converting concentration gradients of eluents suitable for refining protein Low Pressure Gradientor LPG 1000 1 2 3 30 05 5 0mL min 10 9File Link and Loop File 9File Start Stop File 1

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