製鋼スラグと浚渫土により造成した干潟・藻場生態系内の物質
Contents
1. 89 30 40 50 10002. 1 F 1102 18 Oxidation Reduction Potential 500 8 1 450 we 8 0 400 i 7 9 E 350 7 8 iS 300 1 7 250 4 a 7 6 200 r T 7 5 0 20 40 Incubation time days 50 NO2 100 NOB 4 re o 40 80 ay a 3 30 60 Eph Z 40 ro gt 10 d 20 e l 0 0 4e 2 0 20 40 60 0 20 40 60 0 20 40 60 Incubation time days Incubation time days Incubation tim
3. 1 L 700 ml pH 0 85 2 0 mm 3 5
4. I F 1102 3 3 1 1 a i pH 6
5. pH Win Win MS SE et De 6 7 1
6. LIS 1102 39 FAVS eRTICAFTROEOY VIBMADS HABA T EDERA CAET A nf peETE D H DIC pH Re LCMLEbDERSZLI ERM SDICLE p
7. MS 6 7 H25 1 gt gt 1 F 1102 40 gt 1 AARC 12 6 49 51 2013 2 1 24 2012
8. day71 5 cm 10 b 16S r RNA
9. CaO pH
10. 8 2 1 3 100 Ot Bl Sie SER lC REL 8 FR 2012 7 9 2013 6 28
11. 2 Win Win
12. L F L02 2 SUNDEL L Ok DER ER RMI 7 LIBRE LOIEG LECKE LEGAO NI IFZ CBRCOuc SNA RIC ko TRH S464 Vip RB BAE SCE SUP RMRI RRE MWAZA kos AYE 1 21 10
13. 1 2 2
14. INIT a a a pH a
15. 2 6 g em 3 6 g cm 1 F 1102 64 ee cmn Oe 25 1 cm 1500 7485 cn 5 cn a 10 cm 1 cm 1200 Bae de cm A AS 710 cm T 900 an 600 300 0 300 1 cm 1 cm 2F B75 cm 5 cm ee L10 cm 10 cm 200 150 in 100 R 50 t a 0 50 P 100 150 2000 ose cm 1 cm 20 5 cm 5 cm cm 210 cm 1500 eC T 15 D p 1000 H 10 a o Fa Br 500 D 0 012723456 7 8 9101112 Ol esd Be BOP eS A E
16. 2 gt gt 21 10 11
17. 2 KX I F 1102 1 1F 1102 1
18. Si0 Si pH pH 5 cm pH pH10 1 5 379
19. k ar Fi 1 1 2 2
20. 3 1 RR ALFRERBAOR MO 4 100 m 1 8 9 2 5 cm pH 0RP
21. 2 gt gt 6 21 LAL 10 BE 11
22. pH Win Win 1 gt 1 2 3 4 Satoshi Asaoka Tetsuji Okuda Satoshi Nakai Wataru Nishijima ISIJ International 53 10 1888 1893 2013 Determination method for maximum calcium releasing potential from steel slags marine sands alternatives in seawater B2 69 2 11276 11280 2013
23. 09 6 8 20 30 40 50 100 BHRIDA EL 3
24. pH UO Wale F
25. L102 29 AFTRBEOBIRED 6 OH BAD RW ODO REZIDI DRAEK ERORE amp FIT 0 5 cm day0O day71 0 5 cm 2 5 cm 111 0 5cm day71 0 5cm
26. 1 MER 0 25 g 25 mL 24 1 ii pH 1 1102 4 file SER 2 Sette LUT PED g Ch ARE SER OC yee 7K 2 50 L
27. LF 1102 16 FARE PAL Le FEVERS ClOMOBEDSOMERAZLIATTIZCIA L Mek eT LKROATT BRRIEC ORB RYO ORBBOWMHRSEM 1 12ICRT WWTAAL MVVAFTEBRELOES MD 5S VAMEKPICRH UTI SZ OICH UT M ERREOIRAD amp Ybk FIRE EBE IL HH RRE LIRE CUDNEY BFK BRREROBAFR CH BRED OCHEKAOWUT SVU Y ORL MEKEOY Vai LURK ANSZBRANTYR PO4 umol gr 4 days SiO2 Si 25 0 06 V 0 04 15 oh gt 0 03 Abe foe EVE 450 es A H HU 0 02 7A Q 5 0 01 0 00 S 0 0 2 4 6 8 days days 1 12
28. LF 1102 78 0 8 0 6 iva tal 15 0 4 Hi AU 1X L XU 0 2 tn 0 0 i a OO gt D 1 N wv lt H LO k KG 3 K 2 46 PU VAD DT TAD EeRREODREARI l OF FRE HIAR O KR 2 QE 2 47 2 48 Qy 20 30 20C 30 Qy 2 49 0 406
29. 1 2 1000 ml 700 ml pH 0 85 2 0 mm 3 5 13 7 1 4 pH pH 1
30. 2 11 i 8 9 1011 12 3 4 5 6 2 4 2 5 E Simplisetia erythraeensis Site M O Capitella sp Site NA Prionospio japonica O Pseudopolydora sp oor Musculista senhousia Site NC Laternula sp O Others 0 0 2 0 4 0 6 0 8 1 2 6 AL Fis Site M RUB AF WLS
31. 1F 1102 111 2 1 BME S BMA EV AY Ulva DEB FIB DIP DOP Det P 2 2 BMA Ulva DEB FIB DOP Det P DIP
32. 1F 1102 ii 3 1 6 RIX HEB Rigaku EDXL300 1 3 ICP MS ICP ICP OES ERI
33. 70X150X60 cm 1 2 3 RBA 2012 8 21 2012 11 6 2 4 CR PRE ee ee be CRE BARRIE8 15 25 48 8 15 30 75 BRC OWRA BO 56 mm 21 cm
34. 70 5 cm pH 10 0 1 cm pH 8 5 75 pH 10 12 5 cm pH8 5 pH 5 7 5 cm
35. Win Win
36. 1 F1102 22 1 20 c pH pH pH EET OLR ORE eb OMG gt Finke UCM CES Z Bae Le Be ERR ABR Cl BARA 2 10 30 63 121 2128 B
37. 1 pH UQ MK UK MK OPR 12 UQ MK 2 38 UQ 2 39 MK 2 U0 9 UQ 10 UQ 10 Sp 7 goo7gs 7 oo gc7er
38. x AAT 1 ORT EB IL 4 Ca NH4 oa ay oak einen oni 1 1 t te Y 1 1102 92 2 72 18 8 Ae 48 4 3 x m X m 2 73 2 71 5 1 WATT EERE OESCLEOTE 1 aa LRRMOMAD OMELET 3 100
39. 4 0 45 um Auto Analyzer POP 1 F 1102 48 POP gt lt qe diffusion excretion or Silica Sand 2 6 BMA dBMA DA VF BMA 477 gAZ4 hi 36 IF DEB HEA VF BMA mol Day VF BMA eDIP BMA x mn e BMA et BMA SC et
40. Det P 0 034 uM Mortaliy 0 010 uM day Mortaliy 0 0023 uM day Mortality Feces 3 0 X 107M day Mortality Feces 3 2 X 10 uM day Det P 0 0074 uM Decomposition 0 0016 uM day Decomposition 0 0068 uM day Mortaliy Ben 6 4 X 107uM 0 023 uM day Ingestion 2 5 X 107uM day BMA 0 036 uM Uptake 9 3 X 10 uM day Ingestion 0 024 uM day Uptake 0 052uM day 1 BOSC uM day Diffusion 2 4 X 10 uM day Excretion 0 0020 nM day Diffusion 5 6 X 10 7uM day 9 j y Excretion 0 0011 uM day A Silica Sand a b 2 35 a b 4 UQ MK UQ MK UQ MK UQ MK 2 36 2 37 UQ TMK UQ MK 0 36 mo1L 0 22 umol L
41. 1 F 1102 70 pee MO hen ON ON ae 2 4 VG pH Ca Mg NH N PO P Ri RAFU EY Evy 5 KRAN ie FI SE Hy Skeletonema costatum S Cymbella spp EE Naviculla spp Surirella sp H I RIBE ia 100 e f 8 1 25m 90 E 62 50m 9 0 3 75m 70 1 25m 60 2 50m bn bun O 3 75m so J 40 O 30 E 2IJ RREA Nat Y INS n PN D VATS 0 0 568 300 day 0 1 25m 2 5m 3 75m Day356 2013 06 28 2 31
42. 2 34 c d TAU LORE CH AERO HF Be BL CE fe 2 34 a b 12 a JL 4 5 c 4 0 ZAME 3 5 1 PAM P hmol 100cm 2 to PES umol 100cm Re N N Sa O Sa II 12 b P hmol 100cm WA EPEE umol 100cm 0 2 4 6 8 10 12 14 2 34 a b c d 2 356 U1va BM
43. 2 2 X 1079 0 0045 p 0 041 3 0 x 10 tp p 1 6 x 10 0 0076 0 0029 2
44. pH 3 100
45. pH 30 40 1 F 1102 43
46. 1 pH 1 10 c 1 7 4000 r 20 3500 E 3000 aan ge 2500 os g 3 2000 x 3 JE 3 r Aer ten oo IK O na 4 Zz _ 4000 ic 0 10 F 008 Sp a 0 06 5S E oe ee 2000 8 Ou R 0 02 l R p 1000 000 S N gt 0 02 gt 4 9 0 04 2 0 Fool mmea M oo 5 5 0 07 E 2150 TAAIE 11 R a3 a 2 2100 10 70 05 oe 42 Oe o T R 2050 9 73s 3 003 R ai CO 5 27M a lt 0 01 12 1950 7 0 2 4 6 8 10 0 09 0 1 10 K eM RADTT a dy AIZI ABETELE f KOpH SREY VAY EDRED E URR ED 6K O REO BO RAE b
47. XRF JXA 8200 JEOL LP 1102 9 1 2 ml s Ze RR FE mm s AS He TR FE mint 0 2 0 08 0 09 1 1 0 54 0 63 2 4 1 2 1 36 7 0 J 5 4 2 5 a pH pH
48. 6 2 25 PA ORE Dm Dork 7006 0 0 0 K 0000 PO0 001 0 066 0 26 0 068 bi ih 15 2 0 0 0 5 1 0 Es n 5 abali 5 n 3 dieg Hi 3 456 7 8 910 1112 3 456 7 8 9101112 2 25 Aw EIE
49. pH 2 FREER 2013 PSA DTT FRU TICK OF BRERAORIE 3 GAC 16 2018 1 4 sat BRIER AARC 16 2013 II 5 BRIER PHBH AARC WIS 16 2013 6 BARC WA 16 2013
50. 3 4 1 Fim BBERBAILSIIS4MR TO Of 1 1 29 Li Na Mg Al Si K Ca V Cr Mn Fe Co Ni Cu Zn Ga As Rb Sr Ag Cd Sn Sb Ba Hg Pb B Th U lF 1102 1v pH Kk pH
51. 2 3 2 m 5 mm 20C 2 1 v v Sep Pak plus silica Waters UQ MK PQ 1 F 1102 53 HPLC HPLC HPLC PU 980 CEA ee Cogeo s UVANI S her CHAS obs UVH910 Zee hey 7a 21 ODS Agilent Technologies Z0RBAX ODS 4 6 mmX250 mm 20 pt
52. 2 59 a b 66 7 85 7 2 60 a b 1 F 1102 84 b 20 10 RR 5 0 Po P S Se PS SD P P E L SS D FA ew if ys aN PO WO AN ff F a a ar ye a a a eS E eg e 3 2 59 1 a b a b N MU a W as Fg a a a a gt as as aS os P wa CF a ee Py e 2 60 a 4 X 2 61 a b c
53. 13 7 pH pH 1 E JXA 8200 JEOL BREE 2
54. MK 6 12 pH 6 12 8 71 9 05 1 F 1102 74 0 5 3 5 30 04 B 0 7 4 2 5 gy _ sh 2 0 s gt S 0 2 g 15 Oo 1 0 0 1 Re 0 5 ee 6 0 0 0 l i 0 0 j 7 0 n a 0 2 4 6 8 10 12 month month 2 36 UQ MK 2 37 UQ MK 100 100 80 80 tg 60 60 5 gt 40 40 20 20 0 Oo 0 ae ae S x 2 aN 30O PAS sO 9 Ao RY j Y I Y E MK 6 m MK 7 MK 8 Ea MK 6 H2 MK 7 H2 m MK 8 H2 amp MK 6 H6 m MK 6 H8 E MK 7 H8 amp MK others 2 38 UQ 2 39 MK m UQ 8 UQ 9 UQ 10 UQ 11 H2 E UQ others p gt P u
55. 3 4 5 6 2 1 24 2012 8 1 2005 2 3 4 5 6 7 8 9 1 F 1102 41 2009 Futatsuka T Shitogiden K Miki T Nagasaka T Hino M ISIJ Int 44 753 761 2004 Dissolution behavior of nutrition elements from steelmaking slag into seawater M P Javellana I Jawed Cem Concr Res 12 399 403 1982 Extraction of free lime in Portland cement and clinker by ethylene glycol P Arjunan A Kumar Cem Concer Res 24 343 352 1994 Rapid techniques for determination of free lime and free magnesia in cement clinker and portlandite in hydrat
56. 2 45 3 2 3 1 3 LF 1102 77 2 44 DCS DS SS DS
57. REE 1 7 1 2 eg 722 je 1 0E 06 1 0E 05 1 0E 04 EES SES ESE ETSI 1 0E 03 1 0E 02 mg kg 1 1 0E 01 1__ 1 0E 00 1 0E 01 EF ee eee ee eee eee ee ee eee eee oe a II II I O a a oe oe ee oe ee ee ee ee ee ee ee ee ee Oar ee 0 _ F A eee PF F ee rc noon mr PF F F oe oe oe oe lt oe ee oe oe oe ae Ree ne AC CE eT eT DAC E ICCC ACG ICCC Rn TS Tenn TT Tee eT TD Pe a r r ee ee ee ee ee a r d penta mss DNDN DDD DNDN DNDN NDR RNR DDR RDN DDD DNDN DDD F F J F J A A parnm n n m m n u n m nm n m u m u mou mou munnu mm u n u uou n m nunn m u m u mou nou monnun mn uon u nou n m n E r r r r eo r r oe oe oe r oe oe r oe OF Il PETT e eT eS ee ee aa ee TaT ne a a ea ea DNDN a DE fs Er r r r r r r r ar a ar a J FF d 1 oe ee oe ee oe ae ad FE a ee ee ee a O r a A ee ee ee ee ee ee 1 ET F F a EE F I N F Er r oe oe
58. 13 1 5 3 1 133 cm 1 costatum HRE AS 1318 Pole AR aR BEL 097 cm 31 12 5 1 3 154 cm Ck BE ee A DM Aphanocapsa sp 1 133 TAA cm 31 14 13 1 5 1 Cymbella spp Navicula spp Surirellasp 3
59. 4 5 m 0 68 m 3 100 40 cm 10 mm 0 8 s Ca 2 42 1 Ca C 0 lt C lt 1 Ca 2 43 Pt 8 1
60. 70 1 4 U OSL Abt xz Abt xz Total A B C A B C SiO2 20 00 35 20 2810 17 90 13 00 19 60 AkO 367 485 3 03 447 241 180 Fe203 20 40 16 70 31 50 24 50 27 40 25 10 CaO 41 60 34 50 27 20 38 20 49 40 43 60 MeO 1533 13 136 463 266 170 TiO 100 130 097 123 028 0 57 MnO 123 435 541 333 IT 3 17 P205 4 14 1 23 2 08 2 56 1 58 2 413 LOI 0 11 0 46 n d 0 93 1 50 2 16 S 018 O15 0 07 008 003 0 12 mass LOI loss on ignition n d not detected 0 85 2 00 mo Ki 68 489 110 IZ BEDS 50 4 0 4 2 59 29 Li Na Mg Al Si K Ca V Cr Mn Fe Co Ni Cu Zn Ga As Rb Sr Ag Cd Sn Sb Ba Hg Pb B Th U o IO TCARZIZ MUYATFTFRBEIUY BIRLAOSMIRE ARREARS
61. 2 4 5 2 6 2 7 30 40 2 8 9 PP 2 10 1 1F 1102 vi
62. H PE PONa Mg Ca wet 300 2 5 300 2 5 10 25 wpe _ F F ca gt fe F9 GO GO GO GO GO GO 250 250 bp on uD bO un on 2 Cr Zn 2 8 2 oi Oe i oe 7 oH 200 200 4 H 4 A oA z F 15 amp as 6 15 R gt 150 i gt gt 150 4 D gt D5 gt u ho Ty U X al 1M aM 1 M M4 1 M 100 100 g cC ot g g g E 2 3 a g El fl El El E Fe El 50 Ca Fe_ i 0 36 50 Jx 2 i 0 5 S 2 Ser 0 5 S 0 r o 0 0 O EEE a 0 0 10 20 30 0 10 20 30 0 10 20 30 hrs hrs hrs 1 9 1 M A EROR HE ORRE ii pH pH
63. pH 0 85 4 75mnm 100 2 R PE 0 30 840 mL 24 L 1 5 E 1 5 b
64. 1 6 12 cm Ryzon 12 5 cm 40 cm 80 pH 2014 3 ER 4 2013 2 1 2013 10 11 cm 10 cm 0 8 mm Diatoms gt BER Cyanobacteria Green Algae
65. 1 7 PIN 1 1 1 7 1 10 d 1 1 1 7 1 10 e RATT
66. 2 7 Navier Stokes C
67. staan a emcee Oe 1 F 1102 65 f q ei 5 cm RIC Hoe b OB MYD LK TORDIR O HERH D HA pH 1 2012 6 21 4 2012 8 31 67 2012 10 10 107 q 0 0 0 E O O O Hr Oo oO Oo BK q Ag 2 O O O NM N CN Gt2 3 455 9TtTAS 45 6 O TZ 4 5 46 NO N uM NO N u M NO N u M O Oo oO E iO O ite ph oO oO oO BK q q q is wo ite wo H _ gt a O CA O CM N CM 2013 3 7 259 2013 6 6 350 O O O Oo En 0 o KR
68. 1 2012 4 9 BB LOT VEOReRMAAGA CRE LE COB pHRLOURBROTM AIC ERR 1 cm 0 2 um 16C 20C pH pH QuAAtro 2 HR BLTEC 16S rRNA DNA RNA DNA
69. 10 1 M c et ei 2 1 1102 5 2 AR
70. 8 1 23 day 0 8 0 2cm 486 1 cm 1 1 5 cm 0 1 cm 7 5 cm 0 1 cm 8 day0 8 day1 8 day0 8 day1 8 dayO 8 day1 8 day80 Ca mg L Mg mg L Fe ug L O 1000 2000 3000 0 500 1000 1500 0 5 10 15 20 bulk bulk
71. 41 BHRR HERE 19 1 F 1102 98 in 2014 42 UR RK RBA IR 2014 TI 43 EFA KU 48 2014 44 79 2014 45 RHA T The 11th Japan China Symposium on Water Environment 11 Xiamen China 2014 Benthic community emerging on the artificial tidal flat constructed using a c steelmaking slag 46 Tetsuji OKUDA First China Japan CAS JST Workshop on New Environmentally Sustainable 79 Syste
72. 90 x 110cm 67 cm 2 3 18 cm 12 5 cm 40 cm 80 2 2 2 3 2010 3 3 65 mm PVC 25x25x25cm 3
73. LVOEBREOBSMKSAWENMMSNS NOz NO N uM NHL N uM PO P uM SiO Si uM 0 10 20 0 400 800 0 20 40 60 0 400 800 1200 j 0 0 a Slag A A ie oF 2 S S a l l aa 15 15 pH NOz NO N uM NHL N uM PO P uM SiO Si uM 9 9 10 11 10 20 0 400 800 0 20 40 60 0 400 800 1200 0 1 0 a TELLES Depth cm X 1 22 379
74. pH 1 10 1 Ca00 pH 1 10 a b 2 1F 1102 14 pH amp EIS x CaC0 pH pH pH 1 7
75. tiw ER PE RE mite B lt gt 2 3 2 5 23 25 110 600 25 34 300 2
76. 4 8 pH LF 1102 27 Ca mg L Mg mg L1 Fe ug Lt 0 2000 4000 0 500 1000 1500 2000 0 5 10 15 20 bulk i bulk i i bulk seawater seawater seawater 0 rg 0 0 24 Oo 20 2 4 4 4 g 6 4 Og 6 2 LZ 2 8 c 8 8 a a l a 10 10 10 12 4 a 12 12 14 14 M 14 16 4 m 16 4 16 18 18 18 8 day0 8 day2 8 day212 Ca mg L Mg mg L Fe ug L 0 2000 4000 O 500 1000 1500 2000 O 5 10 15 20 bulk bulk bulk o seawater seawater seawater 0 0 9 P 2 2 a H lt T 4 4 a 4 og E E m lt lt a HI 10 10 10 12 1 14 14 16 16 d m 18 18 1 24
77. 40 60 pH 11 5 10 5 11 0 100 m 50 10 0 10 5 i 40 lt 30 i A A 10 0 20 10 9 5 9 5 9 0 Ga 9 0 fi Q 8 5 gt 8 5 lt 8 0 a 8 0 ae 75 7 0 7 0 6 5 65 4 6 0 6 0 T 11 5 400 11 0 4 100 60 40 20 300 a 10 5 O 7K Bie 8 isi 200 4 gt 100 Gi 100 200 300 400 T 0 20 40 60 80 100 12 E z 10 LS 4 R 8 12 we 6 4 2 A l 2 m 0 O 20 40 60 0 20 40 60 80 100 days 1 40 SHED KA LEO wHKRARICKITS p HEL 2 41 3 1 F 1102 38 pH
78. pH 9 64 6 22 7 60 8 17 Be pH pH Leo HE 2 pH 0 4 1 6 1 UY ATT RRA ER T HORA Y SID ERM eARL I7AM BRLIESGLE T 0 7 uM N
79. 1 2 1 6 LF 1102 10 VER DIR WD GRIC RIE TH BST OED RR SSH RD ORCI MEN ATi ZAYIF REALE 1 3 1 3 3 yw V z 7K 8 100 L gt 110 mm 1 6 CF LAK GARR TII HSM AD Te ve Ce LCR PAR C50 70 909I wR ELUTE EOAR 0 10 day 6 7L hr 39 Fit 26 7L hr 10
80. Cymbella spp p 0 023 0 cm 0 33 cm vs 33 cm 0 4800 cm Navicula spp p 0 021 33600 cm 96 52800 cm vs 50800 cm 22200 83067 cm Surirella sp p 0 00399 0 cm 0 67 cm vs 233 cm 0 367 cm H ToT Tes LF 1102 69 ag 28 1 25 3 75 m 90 95 ng g Hh 2 lt 9
81. xB ER 4A 7A EH 2 S E oO 1 8 6 z Z4 x 0 p ce ce eo el of sk so el 7 S E SF E F SF SF SF S g ie we e rs s we a A N J lt x X 0 0 1 1 N K K pa 2 10 1 2 11 5 1
82. 75 6 7 Tee 3 AFT gt RRCOREELBOLOOKHRRORBEAER a 8 1 25 EDR
83. 0 12 nmol g Di E E ERIH 316 3 1 4 umolP g 384 9 6 9 umolP g 7 80 1 umolP g 1 11 ii a ii a 1 10 d e f BUR AIL 1 HADITH DIC ROR bOD 77 WIRE 1 7 NO ITZLWA RZD B
84. pH 30 40 i a N x By E ZD S 9 LH 1102 93 Hb OMmRe HC FIs ROERICH SN SMA TTR bRMNT pA Oo hee AY
85. pH Q 40 7 4 8 5 8 1 9 0 pH Eh 100 10 100 50 10 30 20 40 100 10 pH Eh 3 51 HFR 55H KIA VW 58 pH Eh 1 41 pH
86. 2 E Simplisetia erythraeensis Site M O Capitella sp Site NA E Prionospio japonica O Pseudopolydora sp ene Musculista senhousia Site NC E Laternula sp O Others 0 0 2 0 4 0 6 0 8 1 2 21 ALFI Site M Site NA NC 1 F 1102 63 Simplisetia erythraeensis WN Pseudopolydorasp a Musculista senhousia Capitella sp Other Polychaetes Other Bivalves m Prionospio japonica E Laternulasp Crustaceans Dec 2012 Aug 2012 May 2012 Mar 2012 Dec 2011 Sep 2011 Jun 2011 Mar 2011 Dec 2010 Sep 2010 Jun 2010 0 200 400 600 800 ind M 2 22 ALFI Site M 2 1 1 2 23 pH 10 cm pH
87. 2 5 pH pH Ca 2 10 1 pH pH Ca pH
88. 415 3 mg 100 cm 0 34 mg 100 cm 1 200 RI 2 28 2012 7 9 14 2013 5 17 326 13 4 36 CER AM S02 SA HE EROS SARE 2 1 4 30 CE N26 27 EE ea RW 25 BEE 1 1 3 30 EE e 6 3 EMI EE OPES MERU MK Eb MMIC BT Oa 87 4 Cymatopleura solea Odontella sp Skeletonema costatum Synedra spp i fj 1 Distephanus speculum 7 7 hAl Haptophyceae SNEDE EER AMEE Cyclotella spp Eucampia zodiacus Hyalodiscus stelligera Thalassionema nitzschioides BE weil Aphanocapsa sp EQ E MM1 Dictyocha Tibiila Cayo 1c bo 2 26 7 AU RARE OD ER Ze 2 FAIR dat AZZ iB I F 1102 68
89. gt gt TARI 70 uM Si T 7 16S rRNA 1 26 Proteobacteria DNA DNA RNA RNA 1 F 1102 30 1 6 As
90. 10 1 cm 2 86 75 w w dry 21 Li Na Mg A1 K Ca V Cr Mn Fe Co Ga As Rb Sr Cd Cs Ba Hg Pb U 0O 212 AFAR
91. ga 7 Bentho Torch bbe Moldaenke 2 17 5 2 2 e 4 1 1 n 35 pH 6 92 8 08 0 44 3 29 kNm 68 816
92. 1 24 8 75 pH 8 0 10 5 pH 8 3cm 3 cm 75 3 cm 3 cm 4
93. 14 14 24 10 24 w w 50 Mg A1 Si Ca V Cr Mn Fe Zn Sr Mg A1 Si Ca Mn Zn Sr 50 Na Mg 6a
94. T P Site M T P 0 8m Site NACNC T P 1 2m 2 22 Site M 2010 3 3 9 Shannon Wiener Simpson 9 26 1
95. 10 X 2012 10 1 98 2013 6 28 368 1 1 10 X 10 X 10 cm 3 1mm 70 2012 10 2013 6 6 CCRT PN 5 sa 2 3 1 25 2 5 3 75 m 3 1X1 cm 5 mm a 90
96. II 20 HAT SARC BEER 16 2013 21 7 2013 22 RWS in 2013 23 Akiyama Y Yano H Katayama T Koba K Nagao M Okuda T Nakai S Tanimoto T Yamamoto T Nishijima W ISWA World Congress Vienna 2013 Vienna Austria 2013 Impact of steel slag on benthic community under semi artificial environment 24 Kenji SUGIMOTO Yoichi NAKANO Tetsuji OKUDA Satoshi NAKAI Wataru NISHIJIMA Mitsumasa OKADA EMECS10 MEDCOAST2013 Joint Conference Marmaris Turkey 2013 Changes in dominant species of seagrass bed on eastern Yamaguchi Seto Inland Sea Japan 25 Akiyama Y Yano H Katayama T Okuda T Nakai S Nishijima W EMECS10 MEDCOAST2013 Joint Conference Marmaris Turkey 2013 Effect of steelmaking slag
97. 4 2 18 1 F 1102 60 pH ORP 2 p gt 0 05 p lt 0 05 2 3
98. p5H Ai GE HEA D BRE S EE Ik 8 15 ME 30 A 75 1F 1102 29 1 5 8 15 30 75 8 15 30 75 6 1292 1678 3031 560 1009 1446 2889 mmol m i Eee 0 1 27 7 78 62 9 8 95 21 2 40 3 154 mmol m py E 97 283 498 1234 210 260 418 1644 mmol m ADT BUR TE TBR D gt b 4 JB BSD DS TE BRT BRK NAH DS AS HT Se DIC ERE UR EBB EL U48 w w dry 0 80 0 18cm 21 Li Na Mg Al K Ca V Cr Mn Fe Co Ga As Rb Sr Cd Cs Ba Hg Pb
99. pH 0RP pH 200 m 2 19 1 mm 2 5 2 0 a b 2 18 4 F RA a b 2 3 MILA p 5
100. 100 2 4 2 14 1 2 86 7 66 7 4 100 7 85 7 9 5 66 7 11 5 1 86 7 66 7 2 4 85 7 7 66 7 10 1 2 56 a b 100 4 100 39 5 cm 1 10 cm
101. pH 21 07 1 35 1 1102 35 HS CHU VAT TOR Cd D SDB EVA DO EPMAD 7c Ba 1 34 amp ARRIBA RIC Lk SLT MR a meaty CPS oie Ce Cl VI PR E 1 38 oe FTC FAR AICHE EDS lt A 15
102. 2011 Wataru Nishijima 40 2012 Tottori 6 Satoshi Asaoka Koji Yoshitsugu Tetsuji Okuda Satoshi Nakai The 10th Expert Meeting on Solid Waste Management in Asia and Pacific Islands 7 Japan 2012 Evaluation of leaching potential of Ca in steel slag for its reuse in marine environment 8 Wataru Nishijima Hitomi Yano Satoshi Asaoka Tetsuji Okuda Satoshi Nakai and Mitsumasa Okada The 27th International Conference on Solid Waste Technology and Management Philadelphia USA 2012 Solidification behavior and control of steel slag in marine environment Tetsuji OKUDA Satoshi NAKAI Wataru NISHI JIMA Satoshi ASAOKA Hitomi YANO Mitsumasa OKADA The 27th International Conference on Solid Waste Technology and Philadelphia USA 2012 9 Long term physical and chemical behaviors of steel slags in sea water and sediment Management SN PHA a 14 10 2012
103. http www maff go jp j seisan kankyo hozen_type h_sehi_kizyun pdf gum23 pdf 2014 5 5 2004 5 Aberle N M Beutler C Moldaenke and K H Wiltshire Archiv ftir Hydrobiologie 167 575 592 2006 Spectral fingerprinting for specific algal groups on sediments in situ a new sensor Takai N Y Mishima A Yorozu and A Hoshika Limnol Oceanogr 47 3 730 741 2002 Carbon sources for demersal fish in the western Seto Inland Sea Japan examined by 13C and 15N analyses LF 1102 100 Ecological Evaluation and Material Flow Analysis of Tidal Flat and Eelgrass Bed Ecosystems Constructed with Steel Slag and Dredged Material Principal Investigator Wataru NISHIJIMA Institution Hiroshima University 1 5 3 Kagamiyama Higashi Hiroshima Hiroshima JAPAN Tel 81 82 424 6197 Fax 81 82 424 4351 E mail wataru hiroshima u ac jp Cooperated by Environmental Research amp Management Center Hiroshima University Abstract For restration and re establishment of tidal flats and eelgrass beds sea river and mountain sands have been used whereas mining of sea sand is being prohibited in some prefectures in Japan to avoid disturbance of the coastal ecosystem Therefore steel slags have been focused as a sand al
104. 92 85 120 70 25 25 2013 5 2 170 190 12 25 DIN POP 2 63 a b DIN GR S6 cm 800 uM 2013 6 6 cm 100 uM PO P GDIN DIN P0 P 1 F 1102 86 160 140 120 100 5 g PX wp 80 i 60 gt NM 0A ZERE NR A ju e5 BAS m mEt Ny R ASET 7
105. Wii A TF 7 50 BIR E50 0 WR AT 7T85 t RIRE15 O BU VAT 750 BER E50 A BU VAT 785 RRE O ACA RRR AT VICKUSZAFTHAWILRERELDSOKA MMH IC 10 13 a b 1 WER ACOAFPFRIORREREH DSO 1 2
106. 48 mm PVC 5 cm 2 140 mm PVC 5 cm 70 2010 3 3 1 F 1102 44 65 m PVC
107. DETERE MS mboy he HOOK 12 c d 4 92 8 92 8 85 15 70 30 25 75 LES 1102509 HOD PER GREE 100 2 17 2012 11 90xX110cm 67 cm 18 cm
108. 1 F 1102 67 10 3 3 2 26 10 2 27 KERHO 3 6 50 2
109. 2012 9 S Nakai Tetsuji Okuda Wataru NISHIJIMA Mitsumasa Okada 50th ECSA Estuarine Coastal and Shelf Science Conference O11 2 June 3 7 Venice Italy 2012 Settlement of clam larvae onto slags derived from steel manufacturing 10 Satoshi Asaoka Hitomi Yano Tetsuji Okuda Satoshi Nakai Wataru NISHIJIMA Mitsumasa Okada 50th ECSA Estuarine Coastal and Shelf Science Conference 011 2 June 3 7 Venice Italy 2012 Determination method for maximum calcium dissolving potential of steel slags 11 YANO Hitomi Satoshi ASAOKA Tetsuji Okuda Satoshi Nakai Wataru Nishijima Water and Environment Technology Conference 2012 WET2012 June 29 2B 12 Tokyo 2012 Physicochemical and biological properties of steel slag bed mixed with dredged material 12 Wataru Nishijima Hitomi Yano Satoshi Asaoka Tetsuji Okuda Satoshi Nakai and Mitsumasa Okada 2012 ISWA World Solid Waste P 8 September Florence Italy 2012 Evaluation of Steel Slag as an Alternative Material of Sand for Coastal Ecosystem Creation 13 15 2012 9 2012 lt gt 14 Hitomi Yano Y Akiyama Satoshi Asaoka T
110. 1F 1102 87 4 7 2 4 E 1 t ol ol of of ol ok ok ole clo S S S S X y K F K _ X x x x 7 y 4 K X 9 8 2 64 1 4 95 cmnw 2 65 a b 4 11 4 4 a b PP ci al Ss al cel x RY 5 KV a lt I y Fer gr E A SSNAN ORE ED ORAM mIRE SD X x N Y 9 4 a g 2 65 4
111. 11 46 2012 niin ak ie 12 S Nakai Tetsuji Okuda Wataru NISHIJIMA Mitsumasa Okada 50th ECSA Estuarine Coastal and Shelf Science Conference 011 2 June 3 7 Venice Italy 2012 Settlement of clam larvae onto slags derived from steel manufacturing Satoshi Nakai Wataru NISHIJIMA 011 Tetsuji Okuda Satoshi Asaoka Hitomi Yano 50th ECSA Estuarine Coastal and Shelf Science Conference 13 Mitsumasa Okada 2 Sune 3 7 Venice Italy 2012 Determination method for maximum calcium dissolving potential of steel slags 14 Satoshi ASAOKA Tetsuji Okuda Hitomi YANO Kouji YOSHITSUGU Satoshi NAKAI Kenji SUGIMOTO Daijirou Matsunami Yorihide ASAOKA Mitsumasa Wataru NISHI JIMA The 6th International Conference on Environmental Science and Technology 2012 USA 2012 Texas OKADA p64 914 June 25 29 Houston Characteristics Changes of By products Origin Sand alternatives in Sea Water and Wataru Nishijima Water Satoshi Nakai Sediment Tetsuji Okuda YANO Hitomi Satoshi ASAOKA 15 16 17 18 19 20 21 22 23 25 26 27 1 F 1102 96 and Environment Technology Conference 2012 WET2012 June 29 2B 12 Tokyo 2012 Physicochemical and biological properties of steel slag bed mixed with dredged mater
112. gt 1 Satoshi Asaoka Tetsuji Okuda Satoshi Nakai Wataru Nishijima ISIJ International 53 10 1888 1893 2013 Determination method for maximum calcium releasing potential fromsteel slags marine sands alternatives in seawater 1 F 1102 94 2 B2 69 2 I_1276 I_1280 2013 CO2 3 WataruNishijima Yoichi Nakano Satoshi Nakai Tetsuji Okuda Tsuyoshi Imai Mitsumasa Okada Marine Pollution Bulletin 82 1 2 101 108 2014 Macrobenthic succession and characteristics of a man made intertidal sandflat constructed in the diversion channel of the Ohta River Estuary 4 Tetsuji Okuda Satoshi Asaoka Hitomi Yano Kouji Yoshitsugu Satoshi Nakai Wataru Nishijima Kenji Sugimoto DaijirouMatsunami Yorihide Asaoka Mitsumasa Okada Chemosphere 111 164 168 2014 Chemical Behavior of Sand Alternatives in the Marine Environment gt gt 1 Akiyama Y B Yano H Katayama T Takeda M Okuda T Nakai S Nishijima W Proceedings of EMECS10 MEDCOAST2013 Joint Conference 875 884 2013 Effects of steelmaking slag
113. 2 70 HAT P RIC MATT 86 48 2 5 m LF 1102 91 Ca Fe NH N PO0 P 1 4 1 2 08 N 0 6 0 4 0 2 100 200 300 400 x m 2 70 Be Ya O at oe TH tak XI 2 71 4 R E8 PEAR 48 OF 1 2 72 2 73 2 WH BHLIOGFSI EE
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116. PH Li H1102209 3 HR AORR SME LCRRATT eGR ULCER LEFRRELERA TI I 1 Ca0 Ca0 Ca0 10 kg CO0 1 L min CO 50 g kg 20 g kg RREA 5 2013 10 6
117. X Rigaku EDXL300 1 3 2011 9 8 2 0 2 um 4 ICP MS ICP ICP OES
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123. CO2 Wataru Nishijima Yoichi Nakano Satoshi Nakai Tetsuji Okuda Tsuyoshi Imai Mitsumasa Okada Marine Pollution Bulletin 82 1 2 101 108 2014 Macrobenthic succession and characteristics of a man made intertidal sandflat constructed in the diversion channel of the Ohta River Estuary Tetsuji Okuda Satoshi Asaoka Hitomi Yano Kouji Yoshitsugu Satoshi Nakai Wataru Nishijima Kenji Sugimoto Daijirou Matsunami Yorihide Asaoka Mitsumasa Okada Chemosphere 111 164 168 2014 Chemical Behavior of Sand Alternatives in the Marine Environment 2 1 23 1P 1102 1 x 2011 2 Satoshi Nakai Esteban R Mino Tomohiro Kose Tetsuji Okuda Wataru Nishijima and Mitsumasa Okada Dioxins 2011 Brussels Belgium 2011 PHOTODEGRADATION OF PERFLUOROOCTANOIC ACID USING Fe II III IN CONBINATION WITH CHELATING AGENTS 3 FHER
124. 1 10 Ca 1 8 4 9 4 1500 mg 1 1 Mg 10 cm 0 1 2 23 e 2 10 8 9 kN m 4 5 kN m 2
125. 2 1 Vmax 12 24 27 42 Ks 9 46 10 79 5 dC 2 1 symbols Definitions Value Lnit Reference te Coefficient of termperture effect HB KtOLDetP pap Decomposition rate nf DetP to DOP at oo 0 000008 day tuning KtO DetP pp Decomposition rate of DetP to DIP at OC 0 00002 day tuning KtOT DOP ye Decomposition rate of DOP to DIP at orc 0 0005 day tuning DLFP Diffusion coefficient of phosphorus 4 20E 05 mnlP day Wang et al 2003b OLSMAJ Growth rate nf benthic micro algae at OC 0 01 m day Wang et al 2003b Ks BMA Half saturation constant for phosphorus uptake of benthic micro algae 45 umalP day EEI 2004 MtOL BMA Mortality rate of benthic micro algae at OC 0 02 day IRF 2004 VinaxLUlva Maximum growth rate of Ulva 27 49 m day Luo et al 2017 KsLUlua Half saturatinn constant for phosphorus uptake of Ulva 9 46 umolP day Luo et al 2012 INtOL FB Maximum growth rate of filter feeder bgnthns at O C O01 day tuning MtOLFIB Mortality rate of filter feeder benthos at 0 C 0 001 day Pape et al 1999 W
126. 1 0 E Z 05 3 0 0 K N A 0 5 iN N 10 1 5 YP AY Rome 2 33 FREYI FOAADRMRRKRAIC TOR VOR WAR R LF 1102 72 NS ES a Lee 2014 1 80 75 DIZ EEDD ei A 2 34 a
127. 1 6 12 cm pH pH 85 92 12 cm pH 1 9 1 cm pH 8 5 1 92 8 1 F 1102 85 2012 11 pH 12cm e 0 Ete H 1cm re HEME 15 6 j ene Jat 1 m eH 30 gt AP x sO aa SESE 175 96 as s Ete A Et ESE A15 S o HE 30 4 AbD 7596 1009 6 6 6 2012 11 2013 2 2013 5 2013 8 2013 11 2014 2 2014 2012 11 2013 2 2013 5 2013 8 2013 11 2014 2 2014 2012 11 2013 2 2013 5 2013 8 2013 11 2014 2 2014 5 2 61 lcm a 6cm b 12cm c pH
128. 12000 12000 T T 10000 10000 5 O16 92H o3f 018 2 ao3f 8000 8000 W 5000 I ooo 8 H 4000 H 4000 H H 8 2000 18 2000 0 0 a b 2 45 3 a b 4 1 D amp TRS FRA ARO GR eX 2 46IC BT PREZO 8 50 100MM FIG 100 TRR EO A 0 3kg 20 40 6 FT CLE 120 SILO 569 kg 30 0 613 kg 40 0 587 kg 0 590 kg 0 347 kg
129. FO INS 379 1 15 379 379 5 cm Wb 25 ER E75 1 15 44 31 34 LF 1102 19 IKE IKE 0 10 20 20 40 4 amp 0 0 10 20 20 40 50 0 mh x 0 TES lt E x lt lt lt _ 6 x _ 6 x 5 5 5 lt T T a x a 15 12 bed A 18 18 1
130. ICP MS FI ICKY FRAME LU pH ARR TAY E 24 7 pH
131. 1 19 a b 1 2 cm 1 1 19 a 2cmn 10cm 80 19 c d 1 80 1 20 80 1
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136. ORAE RROME EY WR RE sy 5000 5000 yy RRL 15 7 A7 RRS sa 4h Rats RY 0 1cm apa w 5 7m 44 PAB BRLIS 00 Y OL em Buy Retin 10 TAPHR HS 10 SEEE 3 ue yt tt 3 By eaEta0n FAB BRt30 eR REE 2300 4p ae RE EN Hyatt a H EO 3 8 8 vi p N Z 8 1000 100 4 7 7 6 6 0 0 0 100 200 300 400 0 100 200 300 400 0 100 200 300 400 0 100 200 300 400 Days Davs Days Days 12 12 I7RB S 74H 5 75 cm 7 RBS BY Y 575cm 3000 5000 11 TRES roms ham HEr 10 A RL IS T o a oe q RY STScm ep ay T 74 RRE zo LT 5000 2 3000 Bi 84754 54th RBLISN 5 am a ee m SRS 3 ot Min ei 2200 en Yt RELI U 3000 gt Y Q RYY RRLI5 8 0 By i BRET e HELIN 1000 ae RY RETIN em By E Ea wT BHRR 74 RELISH MM 0 100 200 300 400 0 100 200 300 400 0 100 200 300 400 0 100 200 300 400 Days Days Days Das 12 12 AVRB Z 74H 10 12 5 cm 10 12 5 cm PV RB A TAB 10125m 4 378 4 Hy RRLen E RtI BIY 05m Ri RE t 15 ae RY RETIN S 10 RI 10 q 9 TAB RELI pn a ae P ia TAB RELIO 6 Am 3 Hh V RELIG FAH RRtIH 8 a Y 3000 gt 8 8 By Ratton 5 8 T BY RRtISN HR HR o LN i orth 0 BHA
137. 6 8 40 cm 2 62 a 1 70 cm 1 92 70 2013 6 70 100 cm 259 85 25 6 160 cmE TRR LZ FV ERIEM 7 11 25 2 62 b 80 1
138. 1400 x 650x 270 mm 2011 10 7 1cm 1 2 5 cm 50 cm 6 pH 2012 5 5 2013 2 23cmX10cm 0 8 mm 2011 10 31 l 2011 11 14 2 16 WY XAT LARAI Y HOTAR a
139. 2 9 6 EX 6 5 54m 8 8 3 FRR 3 21 2 1 1 0 UE Dec Jan FebMar AprMay Jun Jul Dec Jan FebMar AprMay Jun Jul 6 6 51 5 4 4 15 3 3 2 2 1 1 0 0 i Dec Jan FebMar Apr May Jun Jul Dec Jan FebMar Apr May Jun Jul 6 6 51 5 4 4 30 3 3 2 2 1 1 j Dec Jan FebMar Apr May Jurn Jul j Dec Jan FebMar Apr May Jun Jul 6 6 5 5 4 4 79 3 3 2 2 1 1 0 hr D Dec Jan FebMar AprMay Jun Jul Dec Jan FebMar AprMay Jun Jul 6 G Ji 4 4 100 3 3 2 2 11 1 kiat a aa aa E E a Dec Jan FebMar Apr May Jun Jul Dec Jan FebMar Apr May Jun Jul 2 69 ug em 5
140. D KEER L LT BEHE BMA Ulva DEB FIB Me DIP DOP Det P 2 6 BMA Ulva DEB FIB DOP Det P DIP 2013 6 28 7 3 cm 33 4 cm 1 25 2 50 3 75 m 1cm 1 12
141. 1 1 1I F 1102 33 pH A pH 9 Tris HC1 100 mM Tris HCl 100 mM 1 100 1 30 6 2 5 pH Ca wt pH Ca wt h a RZ FZ b 1 30 pH b 2
142. TER Na mg L Na mg L Ca mg L Ca mg L 0 5000 10000 15000 0 5000 10000 15000 0 1000 2000 bulk 0 1000 2000 bulk gt bulk gt bulk gt seawater nn SO ater seawater seawater 0 0 0 0 2 2 2 2 4 4 4 4 gt 6 6 6 6 10 10 lt 10 k 10 5 a 5 1 12 12 12 2 12 14 14 14 14 day0 l dayO 16 16 dayo 16 y 16 day0 18 day71 18 day71 18 day71 18 day71 20 day111 20 day111 p 20 day111 20 day111 22 22 22 22 EMA i HPR Mg mg L Mg mg L Fe ug L Fe ug L 0 1000 2000 puik 0 1000 2000 0 5 10 15 20 25 puk 0 200 400 bulk gt seawater bulk Et seawater lt seawater ea o ____ n 0 0 io 2 2 2 2 dayO 4 4 4 4 day71 day111 6 6 6 6 8 8 5 8 8 10 10 lt 10 10 amp 12 F 12 v 12 e day0 12 e dayO 14 day71 14 14 day71 14 16 day111 16 day0 16 day111 i 18 18 day71 18 i 18 20 20 day111 gt 20 20 22 22 1 25 KY ATAF RREBIOEW RRERAGL Be AO RKTBY salt OF REC
143. B TADIA D C uM N IM N uM P IM S SD MN SD MN SD MN SD A 2006 00 B 71 99 003 C 20 34 007 D 67 29 0 07 1 26 DNA Soil DIN A Root 100 E 100 80 80 60 60 40 40 0 0 A A ae A Po RIN A Root 100 100 80 80 60 60 40 40 20 a 20 A B A G m Other m ACI m Acidobacteria m Actinobacteria m AncK6 m Armatimonadetes m BHI80 139 E BRC1 m Bacteroidetes m CD12 m Caldiserica m Caldithrix m Chlamydiae m Cyanobacteria m FCPU426 Fusobacteria m GOUTA4 m Hyd24 12 LD1 NKB19 OP1 m OP8 Planctomycetes m Proteobacteria_Alphaproteobacteria Proteobacteria_Epsilonproteobacteria Proteobacteria_Zetaproteobacteria SC4 TA06 TPD 58 VHS B3 43 WS1 WWE1 Thermi DNA Soil m Chlorobi Deferribacteres Fibrobacteres m IN02 Gemmatimonadetes KSB3 Lentisphaerae m Nitrospirae mw OP11 m OP9 m Poribacteria m Proteobacteria_Betaproteobacteria m Proteobacteria Gammaproteobacteria SAR406 SR 1 TM6 Tenericutes Verrucomicrobia WS2 ZB3 BEDNAR KA DNA Root Chloroflexi m Elusimicrobia Firmicutes m GN04 m H 178 m LCP 89 m MVS 104 m OD1 m OP3 PAUC34f Proteobacteria_Other Proteobacteria_Deltaproteobacteria m Proteobacteria_TA18 SBR1093 Spirochaetes TM7 T
144. 0 508 0 503 0 540mm Site NCISPCALEAKS S FPRALEILE HCN 789mm CHO 2 Site NA NB 0 222 0 123 Site NA Site M 1 F 1102 62 100 FFFH FFFHF FFFH Ho 90 O Site NA A H 80 Site NB wf 70 Site NC 60 eSite M 2 A 50 40 ME 40 30 20 10 g 1 10 100 1000 10000 MIX Cum 2 20 ALF Site M KROBAFIBLE Site NA NC ORZI 2 21 Site M Simplisetia erythraeensis 70 Site M Site NA 10 Site NB NC 40 3 Capitella sp Site M Site M 3
145. 20 RNAI ISOIL for RNA 80C RNA SuperScript III First Strand Synthesis System for RT PCR Invitrogen cDNA DNA cDNA RNA Phusion High Fidelity DNA Polymerase New England Biolabs Ipswich i 16S rRNA V4 PCR 515F 5 GTG CCA GCM GCC GCG GTAA3 806R 5 GGA CTA CVS GGG TAT CTA AT 3 PCR 98C 30 1 98C 10 52C 30 72 15 25 72 5 1 PCR Agencourt AMPure XP Beckman Coulter QIAquick Gel Extrac
146. LF 1102 17 P 250 200 gt 150 gt 100 L 50 15 20 Incubation time hrs l l 10 20 30 Incubation time hrs Incubation time hrs BCL 13 IMER TOZZI ZRELEJ VB a b c UESAC MD BH A c 50 pH 1 14 4 pH
147. Win Win OF MAT 1 2
148. a LF 1102 71 N 2012 10F 12A 257 2013 1 3 2013 4 6 LO 7 71 al POM e i LO i i w AQA H H z ea 2013 1 3 2013 4 6 LO e e e H MM a LO 25 20 15 10 5 25 20 15 10 5 25 20 15 10 5 613C 2 32 3 2 33
149. 29 pH pH pH
150. ks Ulva DIP ellUlva a x exp 1 O 44 Topt Ulva Topt Ulva MF Ulval mol Day MF Ulva MtO Ulvalx exp tex Tis x Ulva x Av MtO U1va 0C Day Det P Der t H HFE RERSY BAICE SHR A SR DOP DIP JER AE SHH Be ORESE NU FADARE NY PZO NY bh AICE GER Eid KF DetP KF DetP op Kt0 DetP x exp te x75 x DetP xV x1 5 KF DetP p Kt0 DetP x exp te x Ts x DetP xV DIP dD _ HRANY hAICESHER ASAE DetP DOP BMA Ulva dt JK HAN DYE 1 DetP DOP BMA OC I 2 y ee p D m e s C mol z m p e o ORE kg m wo BAKE LF 1102 52 oh Fo F formation factor D C m s F D D d ad D 0 m e s7 a 0 048 0 040
151. 700 gL TMP 0 5 M Tris HCl pH 8 0 1 7 w v K 25 20 mM MgCl 2 000 rpm 2 16 100 G 4 5 2 mL 700 pL 6 vy v pH 8 0 5 mM Tris HCl pH8 0 5 mM EDTA pH 8 0 0 1 w v FF 2 000 rpm 2 16 100 G 4 C 5 2 mL 500 uL pH 8 0 16 100 G 4 5 2 mL 500 pL LF 1102 7 25 24 1 16 100 G 4 C 5 amp 2 mL 500 pL 24 1
152. 75 11cm pH 8 7 8 75 75 1 2 cm day 2 8 F1024 28 2 cm 2 cm 2 2cm pH
153. SEZ 0 47 0 35 0 21 0 43 pH 0 034 0 33 0 86 0 20 ORP 0 24 1 6 X10 0 46 43 X10 0 71 0 34 0 50 0 56 pH 0 67 0 24 0 53 0 67 ORP 0 62 0 88 0 51 0 93 0 68 0 015 0 84 0 70 Ti OK De pH 0 26 0 13 0 60 0 50 1 F 1102 61 ORP 0 028 0 15 0 22 0 38 20 16 12 10 n 2 19 10 pH 2 Site M Site NA NC 2 20 Site M Site NA NB
154. i 1 11 316 umol g 385 umol g LF 1102 15 89 30 40 50 1
155. PHS HE 16 2013 11 Mi 35 RURA PHS AARC 16 2013 36 Kenji SUGIMOTO Yoichi NAKANO Tetsuji OKUDA Satoshi NAKAI Wataru NISHIJIMA Mitsumasa OKADA EMECS10 MEDCOAST2013 Joint Conference Marmaris Turkey 2013 Changes in dominant species of seagrass bed on eastern Yamaguchi Seto Inland Sea Japan 37 Akiyama Y Yano H Katayama T Okuda T Nakai S Nishijima W EMECS10 MEDCOAST2013 Joint Conference Marmaris Turkey 2013 Effect of steelmaking slag on marinebenthos 38 ate fm SH At PH RD 2 4 2013 39 Satoshi NAKAI Tetsuji OKUDA Aquaculture2013 Las Palmas Gran Canaria 2013 Attraction of larval settlement of short necked clam by steel manufacturing slag 40 44 2014
156. Tr war 7 cm 0 8 s cycle oe NH va TEST aaa faint i cf es o AH HEFY KULGA D FE wi gt ps OR A a Lae how saree A AY ar nee 2 3 1 F 1102 46 2 4 100 20 40 60 80 0 mm 2 5 AWH 1 2012 7 9 14 pH ORP Ca MRE 2012 6 21 8 31 10 10 N0 N N0 N NH N P0 P Si0 Si 1 5 10 20 cm NH_ N 2013 6 28 1 F 1102 47 2 2012 10 2 99
157. 1 3 47 9 5 17 8 2 13 0 RS Rt t 0 8 8 20 EM A I L VED 20 20 30 20 a HH T TL 30 MRA L 40 ME IFL 50 x RRt W RE RRE 40 BEt MRIYADT 50 20 30 40 50 60 70 2 50 2 51 1 F 1102 80 2 52 3 1 pH 2 53 a b
158. 5 a pH 48 1 39 Sa ea a Wales Nis caine O A a A A 39 0 7 2 5 kN m CHS 4 4 3 mOh 48h ET Aam eae eo E 3 l Pz amp TR lt i
159. Ca Fe Mg NH N PO0 P pH Ca PHREEQC Parkhurst Appelol 1999 g g 2 2 pH WAM WIR wi PIA ROR CHER Ds CRE LE ATTIRAWORA CK SRHMATTROOBHWO BML 1 F 1102 54 cu _g ox 2 u Cui
160. RNA DNA 0 5 gs 0 2 g 2 mL DNA 20 RNA 80C DNA DNA 700 LOY VB RAK PBS 8 1 mM Na HPO 1 47 mM KH PO 137 mM NaCl 2 68 mM KCl 16 100 G 4 C 5 DNA 1 mL 8 w vyy PBS 16 100 G 4 C 5 DNA FO 1 um BioSpec Product Bartlesville lk 4E A Alc HAIR A
161. X 2 12 FluorPen FP100 2 13 QY 2 14 15 20 0 3 5 cm 100 LK Te BEB UK MIL cm 10 re Peete be i gt Gienia Bese te EUERE 20 PETE EWENNO AL ea APA COORD wC tHE MET 1 F 1102 58 2 14 2 15 3 NARRAR PRICIL FIV TPT ZO BAALABARCAWKEOLMUMUY APT L RREZAO BR 30 cm ME X23 cm X12 cm HS 2 16 3 2
162. 1 5 10 cm 5 cm 1 cm 5 cm 2 5 cm p ICP ICP 60mm x H200mm 70cmX150cmXH60cm 1 2 1 3 3 APT gt BRE ORERBO kK d ORR EM RER a 86
163. on off 100 umol m s1 20 0 5C 30 2 8 2 9 LF 1102 56 AFTADEUEDEE FIC 20 8 20 30 40 50 100 REA OIA e 4 50cm 3 2 2 10 3 2 cm 11 cm m
164. 1F 1102 20 A Bete BRK OD pHs LUERE DIC 1 17 18 RREN ORE EP ORKE E 1 1 ERRORI e mR 1 1 0 1 cm 5 7 5 cm 10 12 5 cm 1 10 cm pH i HORRAKO BROER 1 1 pH
165. 8 4cm 4cm pH 9 5 10 5 75 8 cm pH 7 7 8 7 8 pH 8 75 pH 8 3 cm 3 cm 75
166. 896 47 TD E 15 20 Eh B90 gt i RELI w 3B 100 0 0 2012 11 2013 2 2013 2013 8 2013 11 2014 2 201445 2012 11 2013 2 20135 2013 8 2013 11 2014 2 20144 2 62 a b 1200 60 2012 11 DIN 6cm 2012 11 PO4 P 6cm 1000 89 1596 3096 800 759 gt 4t gt BHRt8 gt 74 BR t15 600 ms gt 441i 30 a AAR t 400 gt A 100 200 0 ee S s 2 63 DIN a amp P0 P b 2013 4 7 1 2 64 1 1 1 5 cm 2 1 cm 1 1 1 5 cm eninge RENA ELOEBCIOKS AIKMDOTIZERM CCH E1100 EDE VAD TEG ER Clk RIREKOIEAIBKEWIE EME No 70 Be CRIB 100 LH LIZIZ UME eRL DZ AP TIBG IED 25 EB CITB 100 E
167. bulk seawater seawater seawater 0 4 071 j 2 2 3 J 4 4 4 ps 6 6 lt 6 8 c 8 c 8 E BO l 8 10 10 4 5 104 12 12 12 14 14 14 16 16 2 16 18 18 18 1 23 CRESIEN EIR 1 F 1102 26 pH 0 2 cm 1 cm 1 0 5 cm 7 5cm day 0
168. 1 pH 85 7 pH 11 4 85 7 66 7 1 1 pH pH 9 0 9 5 2 pH 8 7 LE AR DF D hb Vv BURA TT EERE OIG EO2 57 H 74 2 54 100 3 lt 14 396 33 3 10 MRADT RRL14 3 m 33 396 pH 6 2011 10 2011 11 2011 12 b 1 11 A 143
169. 51 55 58 Eh 20 40 60 1006 90 0RP ORP 90 40 9 7 kN m pH 5 1
170. pH CE H pH NO N0 N NH N 1 F 1102 24 PoP 25
171. 7 8 9 10 11 12 LIS 1102 99 WM 1995 Pape 0 L Jean F Menesguen A Mar Ecol Prog Ser 189 135 147 1999 Pelagic amd benthic trophic chain coupling in a semi enclosed coastal system the Bay of Brest France A modeling approach Wang H Appan A Dulliver J S Water Research 37 3939 3953 2003 Modeling of phosphorus dynamics in aquatic sediment l examination of model performance Min Bo Luoa Feng Liub Zhao Li Xu Ulva prolifera and Ulva linza Aqua Bota 100 18724 2012 Growth and nutrient uptake capacity of two co occurring species FAI 2011 1 th Amelia B Hizon Fradejas Yoichi Nakano Satoshi Nakai Wataru Nishijima Mitsumasa Okada Journal of Water and Environment Technology 7 91 101 2009 Anchorage and resistance to uprooting forces of eelgrass Zostera marina L shoots planted in slag substrates IE 2000 2009
172. E ja 0 40 12 40 0 35 10 35 Z 0 30 Bog 304 0 25 2k 0 6 20 0 20 RR F 0 15 0 4 15A 9 4 10 0 10 20 2 p 5 0 05 0 0 OOO 0 0 00 0 2 4 6 month month 2597 AZT PQ 9 PQ 9 2 40 2 41 PO 9 2 40 PQ 9 2 41 3 1 F 1102 75 6 PQ 9 5 2 42 ERE
173. 3 100 2 2012 6 25 L TRIR 1 F 1102 6 86 ICP MS ICP 0ES b 16S r RNA 4 A KYAI PELEAR E1 3 TIWMOK TAM BRLIEACLE ZB TE E14 3 2012 3 1 70X150X60 cm
174. 333 1 6 2011 10 2011 11 2011 12 2 53 a 1 b pH Li 1102 81 25 74 kg cm 2 54 74 1 LOEAERENSCHICBIB LET Vv ERE 6 2 2 55 a b 100 1 5 2 2 1 66 7 85 7 2 6 2
175. O O 92 E 8 E 92 8 a f 10 gt AR RAST L E R i _ 92 M 8 trn 2 F fi H 5 92 8 HS 2 U _ Sty OO t o 0 5000 10000 15000 20000 25000 30000 35000 40000 15000 20000 25000 30000 35000 40000 1 35 pH 0 5000 10000 1 36 3 3 mm s mm s 1 37 BS THE ED NE Mg b Ca c si d 04 ym Fe 1 F 1102 36 1 38 RREZES LEM VAD THAD AN Fela Si b TIT AR AD 7 DO ARR FO 5 EK DO pHA be Sem E Z HO L EaR S oo Lin AD 792 86 FOC AB 7I vy VRICRRLO Me
176. 1 10 12 5 cm 70 5 cm pH 5 cm pH E 25 1 pH 1 3 10 28 50 80 1 19 1 20 BIRT CEA ULEBROMBRKIZ HUY ATT FAWEDSICRRELAAOKRSEDYRWAT FR 8
177. gt 1 w w Mg Al Si Ca Ti Mn Fe V Cr 1 000 mg kg 100 mg kg Na Al1 Si K Mg Mn Rb Ba 100 mg kg 1 1 2 90 170 40 2 200 23 Li BY Na Mg Al Si K Ca V Cr Mn Fe Co Ni Cu Zn As Rb Sr Cd I Ba U 1 8 0 01 mg kg PA V1 02 12
178. 11 2 Ca0 pH 2
179. 22 2011 4 BRIER 24 2012 pH 5 Tetsuji Okuda Satoshi Asaoka Koji Yoshitsugu Satoshi Nakai Wataru Nishijima The 10th Expert Meeting on Solid Waste Management in Asia and Pacific Islands Tottori Japan 2012 Evaluation of leaching potential of Ca in steel slag for its reuse in marine environment 6 Wataru Nishijima Hitomi Yano Satoshi Asaoka Tetsuji Okuda Satoshi Nakai and Mitsumasa Okada The 27th International Conference on Solid Waste Technology and Management Philadelphia USA 2012 Solidification behavior and control of steel slag in marine environment 7 Satoshi ASAOKA Hitomi YANO Tetsuji OKUDA Satoshi NAKAI Wataru NISHIJIMA Mitsumasa OKADA The 27th International Conference on Solid Waste Technology and Management Philadelphia USA 201 2 Long term physical and chemical behaviors of steel slags in sea water and sediment 8 46
180. 25X25X25cm 3 2 1 2 1 7 7503 13 20 m URIS 50cm 3 0 nS NI eee NG er ee ee PH COMPACT CT 1 Zz 2 1
181. 3 100 ee Sif Yi SER SG COA IL YR BNI A LEM VY AFTT ROTA X 2 3 4 adie 2 3 7 cm pH 0RP pH 0ORP p 0 05 5 86 2012 6 25 368
182. 40 cm 30 20 2011 10 3 100 14 39 JY REL3I3 3 100 Rie RRt14 3 A RRL3I3 3 100 2011 12 2012 2 b 1 2011 10 2011 12 2012 2 2012 4 2 56 a 1 b 1 F 1102 83 14 600 12 BS 500 m 10 400 8 T in 35 zx x 300 0 0 mo Ei a ss ok ok S Si a gt a am a a ww _ xe ak E4 ES Su a 4 4 s i 4 g gr oe Pr eg ye 7 g A 2 57 2 58 2 58 2 5 ps T Ati iR 33 3 BHRT143 100 E333 t143 100 100 1 0 0 4 2 1 2 0 4 0 4 8 3 4 5 4 6 7 7 0 2 6 9 7 5 9 9 1 528 172 388 212 276 252 328 6 9 1 5 4 0 4
183. E A i OPARE E daa M S 3 ond if aR Het g OO 19 o e i 15 2 a po c sr va S 0 0 0 z AARRE B i A 25 A BU Het F 43 m C c PO P uM PO P uM 0 20 40 60 0 20 40 60 35 oH Heer 30 E o p 25 OF m 20 15 E15 eet R Fin D D x 25 A FEDRE i a 43 S 9 a 2 g ry bo r gt 2 2 1 20 1 21 a b 1 c d 80 H a VE ED BRIBES Held F 8 MGR 15 b o A K 25 X 38 48 379 pH 1 22 pH 8 pH pH 11 pH
184. ROP ROR SIUCWR CED S MU YAPTERREOPE Fic CIR BR UY ORM LB AE Clostridiales Kiloniellaceae 20 50 20 r 50 40 H 40 15 4 15 10 a a 10 r a o 7 9 7 20 20 ae Bi 10 98 1 10 O E o 0 0 e D A B D 1 27 BEA OAPRNAIC BW CHU VRI RELER LETHON ROE Clostridiales Firmicutes Clostridia Clostridiales Kiloniellaceae Proteobacteria FH Alphaproteobacteria Kiloniellales Kiloniellaceae uM N 1 F 1102 32 A B WV vAPT BAR EBS LE C D 7 RRE Desulfobacter ales Chromatiales 20 12 10 15 8 I 10 6 4 5 4 0 ee EE gt 0 SS q h A B D A B E D X 1 28 ERF OMPRNAICB YY C74 BIRGER CHE ORDo 7M Desulfobacterales Proteobacteria De
185. U 2001 PAM Pulse Amplitude Modulation AP P100 Fm Fv 3 PAM 30 Ft Qy Fv Fm 3
186. lt 1 0 0 5 10 15 20 30 10 15 RELEASE RELEE a b 1 39 48 pH 8 6 8 3 8 5 pH MIRA TZ CLARK IC BIL lt 12 3 gt 12 7 pH 1 F 1102 37 pH pH b
187. 0379A HZ 1 21 a b c 1 3 11C 8 30 NH4 N uM N 500 1000 1500 oft Hae 8 BUD Hee 15 AR Hee 25 UL HR 48 Depth cm Depth cm NH N uM NH N uM 500 1000 1500 G 500 1000 1500 oo fap 3 me fae 15 as EDEL 25 oy AHL 48 Depth cm Depth cm 1 19 a b 1 c d 80 8 I AR 15 A GR 25 6 X 48 LEP 1102 23 PO4 P uM P uM i 5 OPO4 P a Si02 Si 7 0 0 20 40 60 8 4 0 6 0 ey 5 a ry 1 is z
188. A 3 mm s l a o a C PARRY CE AA OBE 316 3 1 4 umolP g 384 9 6 9 umolP g 7 8 01 umolP g 1 6 1 7 2 3 100
189. B Yh Hr Rik ar Rint oO iN days days i T T I at tre BY FE fir iR i ar 0 2 h 0 L i 1G 20 30 i 10 20 30 days days X 2 48 Ca 3 II 2 44 0 mm 28 mm
190. BMA eDIP BMA POP el BMA age et BMA eDIPLBMA eIIBMA et BMA exp te x Ts DIP DIP BMA ou ks BMA DIP 1 F 1102 49 I I I BMA 1 eN GMA Iopt BMA B MFIBMA mol Day MF BMA MiO BMA x exp te x Ts x BMA x Av MtO BMA 0C Day VFLDEB yy umol day VF DEB IN DEB x DEB x Av IN DEB INtO DEB xexp texTs IN DEB DEB FIB 2 Ye umol P g lt wet weight gt VF DEBAR MF FIB hi3C UF FIBRE EF FIB BEEN HER Det P OR VFLDEB umol day VF DEB IN DEB x SL DEB x DEB x Av IN DEB INIO DEB xexp texTs MF DEB umol day MF DEB MtO DEB x exp tex Ts x DEB x Av Rikt UF DEB umol day UF DEB UtO DEB x exp tex Ts x DEB x Av pkt EF DEB mol da
191. NHL N 1 3 6 gcm 2 6 g cm I 2 32 e OR
192. OZR CURA LU CHR aI K A 1 KE AELA Se WABI EP PUY Ht 7K HAO pH Bk amp BLS Ur He Ft hx 1 1 AIR LE RUBY OP AR SEER O LMS BI b i 1 2 PERS Y 0 2 3 4 5 1 2 ii E
193. w Eis ite Te Te iO EN p R O O O O NM CM NM NS 0 200 400 600 0 200 400 600 0 200 400 600 0 200 400 600 0 200 400 600 NH N b M N H N u M N H4 N u M N H4 N u M N H4 N u M 2012 6 21 4 2012 8 31 67 2012 10 10 107 oO oTe oa uo ww lo se HK o RK ae b a ts zo WO Fe H k nge oO oO N N N G123 456 4123 4856 PO P u M PO P u M ran oO E W WO O 8 hk Bk a a His wo wo lo O O N N N 0 10 20 30 40 50 0 1020 30 40 50 0 10 2030 40 50 SiO Si u M Si0 Si u M SiO Si u M 2 24 Fipvt 7EaAAADAITE NO N N0 N NH N P0 P Si0 Si 2012 6 25 I F 1102 66 2 3 6 3 5 3 7 3 6
194. 0 L 2013 12 3 53 ie 4 1 2 m 1m 1m 1 8 2 8 2 9 12
195. 1 15 16 C Pt 8 Pt 11 15 10 CC 1 1 1 2 42 3 1 1 F 1102 76 4 iB WHE
196. 15 AZ T RR BAMA 379A A ORK ETE ED He RIES OS E 8 15 A 30 x 75 b pH 8 0 8 3 63 pH 1 16 pH pH7 6 7 9 1 16 pH X 1 16 E g pH 7 9 11 0 i 6 8 8 M _ cy n dD A ig 18 1 16 63 pH FF 8 W 15 A 30 X 75
197. 1F 1102 1 1F 1102 23 25 180 437 25 55 519 FXE BRL ERR 1 2 1 21 BHORMLLINEV VES FB 10 11
198. 2 12 6 2 13 4 cm 10 36 cm 9Y 1 2 3 4 4 kF kP kD kQ F P Qy kg r Ch kp kp kg 1 F 1102 57 Fv Fm Kp Fm Fy F Fy E OO Fv Fm Q 2 12 F1luorPen FP100 Fyv Fm Qy QY 2 13
199. 30C12 30 22 30 24 30 50 70 0 50 60 70 80 90 pH pH PRN 41 FUJIWARA ORP ORP RM 20P DKK TOA DO01018 ORP Eh Eh E 206 0 7 t 25 Bh E WEWE t C TEA LF 1102 11 4 1 1 AFT gt BELO PEBBOLOO RMERERER a pH 6 X 1 4
200. 35 9 2 v v 1 mL min UQ 10 275 nm 270 nm PQ 9 258 nn 5 3
201. 7 5 cm 85 7 66 7 2 5 230cm 2 57 58 85 7 100 LERZ I 766 TIA EER CLRID LEO Capitella sp 1I F 1102 82 EE TCTHRIIT VEREADBSPoOKRCLEICEALHRBSNS 5 RHEFATZTERBRIOUMG LEC Bex 100 2011 10 2011 12 2012 2 b A 100 1 Ae SK ett t 14 3 i 14 133 3 xX is A fit 100 gt Fit e Rt E fitm B 333 10 M 8 6 4 2 0 2011 10 2011 12 2012 2 2012 4 2 55 RW a 1 b 80 70 60 50
202. A eee HRP Lal O2 13
203. CHNE AR NO 97 28 Akiyama Y YanoH KatayamaT KobaK NagaoM OkudaT NakaiS Tanimoto T Yamamoto T Nishijima W ISWA World Congress Vienna 2013 Vienna Austria 2013 Impact of steel slag on benthic community under semi artificial environment 29 60 2013 C02 30 25 2013 31 MAER VARS Bie A in WA 2013 32 Bue PAS in 2013 33 KURR KURA PHETT WIR 45 FERR 2013 EIR E ZARI L 34 ARIF Ea FE ee
204. H 2 5 Ca 29 2 gt gt Win Win PRCA SAI EDBRIAENS
205. I R bii i RRLI5 0 0 0 100 200 300 400 0 100 200 300 400 0 100 200 300 400 0 100 200 300 400 Days Days Days Days 1 17 pH 1 18 DIC eT O22 1 8 15 30 5 cm pH pH pH pH pH10 8
206. Site NA NC Det P 0 0074 uM Decomposition 0 0016 uM day Mortaliy 0 0023 uM day Mortality Feces 3 2 X 10 uM day Ben 6 4 X 107 uM Ingestion 2 5 107 uM day BMA 0 036 uM Uptake 9 3 X 107uM day Mortality Feces 3 0 X 107 uM day Mortaliy 0 010 uM day Mortaliy 0 023 uM day Decomposition 0 0068 uM day Ingestion 0 024 uM day Uptake 0 052uM day 1 0030nMGey Diffusion 2 4X10 uM day Excretion 0 0011 uM day ian Se Silica Sand a b 2 7 a b Excretion 0 0020 uM day Diffusion 5 6 X 107uM day F 1102 vii 25 BRIE RMIYADT 0 BRE IFLY 0 8 o He LV 8 EM ARIUY AST 20 lt t ta 20 20 30 20 a 30 40 At IE 40 BRL RMIYADT 50 50 i X Z Rt z ie Ee na ik iR aR 19 5 0 fs 20 30 40 50 60 70 20 30 40 50 60 70 2 8 2 9
207. a 150 100 50 eS se fs ss sor e S R a eo Ko ON X ee g 2 68 1 5 1 a b Takai d 1F 1102 90 2 69 2012 12 1 100
208. a b 1 F 1102 88 2 66 a b 1 95 cmw 10 22 4 2 4 1 2 6 a b 25 Dane ll 20 ia E a 15 10 ili 9 i in xP SS of Lo cf Jol of of el ol Se of o of ee of of of amp DO 6 RS S D O yo S ee at os a ie a SPS Sg SS POP Sa SP OT Pre Oe eS Ot SN AS SS a we Ss ys 4S ow wo E OP PK MR OR y a ge amp SQ 2 66 1 a 2 6 1
209. a International Symposium including Field Workshops Biodiversity in Changing Coastal Waters of Tropical and Subtropical Asia BCCWTSA Amakusa Japan 2012 Distribution of benthic fauna decided in association with sediment environments cc on tidal flat in Hiroshima Japan KUER 47 2013 AGA WAR 47 2013 UA FRR gt RGAE WIR 2013 7 2013 KUER RARE RURE 25 2013 TAM ADT CRUE OIG Ce Re AVY AADI
210. a ei eh YADZ2 1 0E 06 1 0E 05 1 0E 04 1 0E 03 Sr Mo Ag Cd In Sn Sb Cs Ba Hg Pb Th Bi Rb Se V Cr Si K Ca Ti Be Na Mg Al lt 2 lt 3E 2 lt 0 01 lt 0 02 lt 0 3 lt 2 Ti 2 1 7 a LU V Lu T Cu Zn As Rb Sr Cd Na Mg Al Si K Ca V Cr Mn Fe Co Ni B Li 9 800 11 000 mg kg IL GIA RPE 240 mg kg ETC hok Aj it H 1 000 mg kg e A CS SIL oe YO aes gt Leal Z 13 1 WM 1 9
211. e days SiO2 400 300 s 1 14 FANE KRI BUT 5 ARR KEE a pO SS oR ae gt gt aan a pH b WFARKRKRRE c 100 woes g d e f g 0 2 60 0 40 Incubation time days 2 APT RRETORHEROLOOPRERRORRER a Eh mV lk 747 WD BIRREBGLBODOSHRVYVATT R 20 458 mV
212. ee oe oe ee oe oe oe oe oes oe a Prete EEE ney AEE EXE AEE EKE XLE ELE XLE ere TEE ELE TEE TEE O oe oe oe ooo oe oe ml EET ee ONT O oe ee eee O O O oe 1 0E 02 r Mo Ag Cd In Sn Sb Cs Ba Hg Pb Bi Th Li Be Na Mg Al Ca Ti V Cr Mn Fe Co Ni 2 lt 0 3 lt 2 lt 0 02 lt 0 01 lt 2 lt 3E 2 1 1 A amp R ADT MUYAZDFTBLVUBRIODKREAS 1 2 er Me b Ca c Si d D4 Fe 1F 1102 y 1 3 Fe a Si b O 15 O 100 mp 0 92 RRt 8 10 gt s 1 R i o 92 389 8 2 I B 1 i i 9 gt fh RAST 92 8 3 ti gt L 4 a 5 F he 1 i pe E lt i OT poa i 0 oO 1 1 1 1 1 0 Qo 4 l 0 5000 10000 15000 20000 25000 30000 35000 40000 0 5000 10000 15000 20000 25000 30000 35000 40000 1 5 3 mm s 1 4 PRU VY ADO CERAM ACAE I
213. es R N Yong V R Ouhadi Appl Clay Sci 35 238 249 2007 Experimental study on instability of bases on natural and lime cement stabilized clayey soils C Shi R L Day Cem Concr Res 30 607 613 2000 Pozzolanic reaction in the presence of chemical activators Part II Reaction products and mechanism Japan Cement Association Japan Cement Association Standard JCAS I 01 1997 Tokyo 1997 37 59 67 1997 10 Hoch A R Reddy M M Aiken G R Geochimica et Cosmochimica Acta 64 61 72 2000 Calcite crystal growth inhibition by humic substances with emphasis on hydrophobic acids from the Florida Everglades 1 F 1102 42 2 FR RBERBAICKUZERAELBEORH fae R mT 23 24 24 25
214. etsuji Okuda Satoshi Nakai Wataru Nishijima SETAC Asia Pacific Kumamoto Japan 2012 The use of dredged soil as additives to leverage steel slags for restoring sea grass beds and tidal flats b September 15 Y Akiyama T Katayama H Yano T Okuda S Nakai W Nishijima International Symposium including Field Workshops Biodiversity in Changing Coastal Waters of Tropical and Subtropical Asia BCCWTSA Amakusa Japan 2012 Distribution of benthic fauna decided in association with sediment environments on tidal flat in Hiroshima Japan 16 rm 40 2012 17 ca Beek 2013 18 HECK 16 NO 2013 1 19 PEH t BRS RAS BEEK SARC 16 2013
215. h f 0 08 mm 80 gt Tg 60 1 2 mm 40 Ca mg 11 0 10000 20000 39009 0 10000 20000 30000 1 F 1102 34 a b 1 31 pH ag 8 15 S 100 lt 6 T 92 t 8 E RR 10 Ay pi et tS 2 3 2 A 5 _ A ___ 0 i 0 sowe 0 10 000 20 000 30 000 40 000 Di 0 5000 10000 15000 20000 25000 30000 35000 40000 1 32 3 1 33 GUY ATT 3 m s b 1 34 Mg a Ca b Fe c 0 d Mg 3 mm s ON NE eth ay Vat inne age lee eae 10 10 000 1 36
216. hermotogae WPS 2 WS3 Caldithrix Ca bA FIZI RT 16S rRNA Proteobacteria DNA RNA Soil H1102 31 RNA RNA Root A B MUYA2DT EB C D 77AM BEDRSLR RNA 1 27 Firmicutes Clostridia Clostridiales Proteobacteria Alphaproteobacteriami Kiloniellales A Kiloniellaceae 28 Proteobacteria FY Deltaproteobacteria Ail Des
217. ial Wataru Nishijima Hitomi Yano Satoshi Asaoka Tetsuji Okuda Satoshi Nakai and Mitsumasa Okada 2012 ISWA World Solid Waste P 8 September Florence Italy 2012 Evaluation of Steel Slag as an Alternative Material of Sand for Coastal Ecosystem 79 Creation HIR 15 2012 9 2012 lt gt PSS 44 2012 Hitomi Yano Y Akiyama Satoshi Asaoka Tetsuji Okuda Satoshi Nakai Wataru gt h Nishijima SETAC Asia Pacific Kumamoto Japan 2012 The use of dredged soil as additives to leverage steel slags for restoring sea grass beds and tidal flats September SKA RLR KUER 24 2012 Y Akiyama T Katayama H Yano T Okuda S Nakai W Nishijim
218. in 7 2 11 10 RELA TVEOR 2 10 2 11 2 1 20C 2 1C Qy Qy FluorPen FP100 X
219. loP y u Fy g ot a ex pex pP ex ax oer a 0 of he cf ct ox ac g a x aLa EG Sin Sar Re yer _V ue i Fei tq m Pe y rj T h pt Pty o gh p a p s p w 2 7 2 2 EB in 2D 3 xK FH FK N N An Ca Fe NH4 PO4 Mg
220. ltaproteobacteria Desulfobacterales Chromatiale Proteobacteria Gammaproteobacteria Chromatiale A ALB C D y ASIMELBROMMADI AL 1 29 16S rRNA 4 a WHR YY ew CafS tH DB KEW RATT amp AVC Hk e J CASEO E LOM WR amp 577 25 12007 J CAS 12 269 1 30 Ca Ca 28 f Ca
221. ms for Japan and China 2014 Scientific understanding and approach to the management of the Seto Inland Sea 1 Xiamen China 3 4 1 23 C206 2011 8 5 50 IFR RHO AIH 5 6 2 1 22 2011 2 1 46 2012 2 1 in 2013 2 1 24 2013 my 8 1 U87046 1 24 1987 2 3 4 5 6
222. on marinebenthos 2 42 7 398 403 2013 2 1 Satoshi NAKAI Masayuki OZAKI Tetsuji OKUDA Wataru NISHIJIMA and Mitsumasa OKADA The 6th Joint China Japan Chemical Engineering Symposium Wuhan China 2011 Use of waste gypsum for shear thickening of dredged soil by decarburization slag cc 2 PSPs 2 3 2011 3 Satoshi Nakai EstebanR Mino Tomohiro Kose Tetsuji Okuda Wataru Nishijima and Mitsumasa Okada Dioxins 2011 Brussels Belgium 2011 PHOTODEGRADATION OF PERFLUOROOCTANOIC ACID USING Fe II III IN CONBINATION WITH CHELATING AGENTS 4 PHAN 58 2011 Li 1102 95 FKE E amp Win AD D7 HOTERE E O Ha Hh ML BR RARR 22 5
223. on marinebenthos 26 SH PH Ba 24 2013 BRI 27 PHBA The 11th Japan China Symposium on Water Environment 11 Xiamen China 2014 Benthic community emerging on the artificial tidal flat constructed using a steelmaking slag 28 Tetsuji OKUDA First China Japan CAS JST Workshop on New Environmentally Sustainable Systems for Japan and China 1 Xiamen China 2014 7 Scientific understanding and approach to the management of the Seto Inland Sea 7 1 FRE
224. tOLFIB Excretion rate of filter feeder benthas at 0 0 003 day Pape et al 1999 S Max FIB petp Maximum prey limit for DetP in bottom water of filter feeder benthos 0 2 pmolP ig Pape et al 1999 SMin FIG pete Minimum prey limit for DetP in bottom water of filter feeder kenthos 0 005 mol L Pape et al 1999 S Max FE cata Maximum prey limit for benthic micro algae of filter feeder kenthos 100 umolP m lt wet sedimant gt Pape et al 1999 1999 1999 1999 1999 1000 umolP m wet sedimant Pape et al 1999 S Min LFIB aa Minimum prey limit for benthic micro algae of filter feeder kenthos 1999 1999 INtOL DFE Maximum growth rate of deposit feeder benthos at OC 0 05 day Pare et al 1999 MtOLOFE Mortality rate of filter degosit kenthos at 0 C 0 002 day Pare et al 1999 UtOLOFE Excretion rate of filter deposit benthos at 0 C 0 0075 day Pare et al 1999 ELDEB pep Egestion rate for DetP in bottom water of deposit feeder kenthos 0a SRT 2004 ELDEB casa Foestion rate for DetP in bottom water of deposit feeder kenthos 05 Ret 2004 4 PCR
225. tOS Ek ICP MS FI
226. ternative however the slags are known to relase alkaline and cause solidification In addition with regard to use of the slags alone lack of silt and clay as well as organic matters is concerned from the viewpoint of establishing benthic ecosystem On the other hand dredged soil DS is abundant in nutrients silt and clay and organic matters and therefore DS was expected to be a candidate of additives to sandy sediment media to supply these as well as to control physicochemical characteristics of sediment media In order to evaluate the mixture of slags and DS as the sediment media for restration and re establishment of tidal flats and eelgrass beds analyzed here were biological and physicochemical characteristics of the sediment media and material flow of the benthic ecosystems developwed in the artificial tidal flat and eelgrass beds constructed using the DS and slag mixtures We carried out rigorous chemical analyses of the dephosphorised and decarbulization slags and their immersion tests into sea fresh water with without tidal action In the control experiments silica sand was used A series of results showed the materials flow such as behaviors of alkaline carbon nutrients and redox agents caused by certain chemical interactions between the slags and DS and microbial activities In addition mechanisms of the slag solidification and its mitigation by the addition of DS were revealed Furthermore the ealgrass beds and
227. tidal flat simulator tests confirmed the usability of the slags and DS mixtures as the basal media where the nutrient release from the dephosphorised slag and DS mitigation of pH increase and solidification of the slags by the DS addition and resultant growth of eelgrass or migration of benthic algae and benthos were involved Finally we constructed the mathematical simulation models to expect behaviors of the slags eluting components and resultant affects on the ecosystems developwed in LF 1102 101 the artificial tidal flat constructed using the slags and DS
228. tion Kit QIAGEN PCR Quant iTW PicoGreen dsDNA Reagent and Kits Molecular Probe VersaFluor Fluorometer Bio Rad GoTaq qPCR Master Mix Promega RT PCR MyIQ2 Bio Rad qPCR qPCR P5 5 AAT GAT ACG GCG ACC ACC GAG AT 3 P7 5 CAA GCA GAA GAC GGC ATA CGA 3 2 nM Miseq Illumina 16S rRNA V4 PCR QIIMEB Green Genes 4 a Ca 7 pH 7 Ca Ff Ca J CAS 1 01 1997 W 1 f Ca
229. ulfobacterales Proteobacteria FY Gammaproteobacteria Wi Chromatiale 29 1 29 MAMIE MUYAITT BRRIEGELRC TIWMOSZOBRHICHAS LIM VY
230. y EF DEB DEB x Av x V DEB p x E DEB p V DEB xV DEB p 1 F 1102 50 V DEB IN DEB x SL DEB ELDEB HENE VF FIB S fe MF FIB 9 UF FIB JR VE EP FIB Be BE HR Det P VF DEB umol day VF FIB p IN FIB x SL FIB p x FIB x Av IN FIB INtO FIB x exp te x Ts MF FIB umol day MF FIB MtO FIB x exp tex Ts x FIB x Av RHEE UF FIB wu mol day UF FIB UtO FIB x exp te x Ts x FIB x Av GE yt EF FIB umol day EF FIB FIBx Avx V FIBI x EFIB p VIFIB py x V FIB p4 V FIB IN FIB x SL FIB E FIB 0 1 Ulva VF Ulva DE amp hk MF Ulva fE FE VF BMA mol Day VF Ulval VtO Ulva x et Ulva x minCeD P Ulva el Ulwa x Ulvax AV ee VtOlUlva 0 Day et Ulva eDIP Ulva PO P el Ulva l ae 1 F 1102 51 t Ulva eDIP Ulva ellUlva l lt NCNKNCHRDOLE et Ulva exp te x Ts DIP eDIP Ulva
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