シカ雌雄判別キット
Contents
1. PCR LAMP Loop mediated Isothermal Amplification LAMP DNA2. Deer MSDS
3. 1 LAMP DNA Deer
4. A A 240 260 nm 350 370 nm UV 320 nm A B 15 6 1 Yamazaki S Motoi Y
5. 64 C 80 C 0 5 10 ul 10 100 ul 200 1 000 ul e e 1 5 ml 20 m e e 1 5 ml e 0 2 ml k A i UV 240 260 nm 350 370 nm e
6. DNA LAMP DNA Deer DNA Deer Deer Deer 64 C 30 Deer Deer Deer LAMP DNA DNA DNA
7. Deer Deer LAMP Deer
8. Deer Deer DNA DNA 1 MSDS 3
9. Deer 4 0 ul 4 0 ul Deer 1 1 1 x 3 64C 30 12 UV l UV 80 C 2
10. DNA Deer x Deer 10 50 0 ul Deer 64C 5 94C 5 DNA DNA DNA 20 C
11. Deer 14 4 ul 3600 ul 720 0 ul Deer 20 0 pl 40 0 ul 16 0 ul 400 0 ul 8000 ul 1 2 1 Deer 1 Deer Deer 10
12. 2 Deer Deer Deer Deer 20 C 1 x 3 1 5 ml 20 ml 1 x 3
13. NIPPON GENE e TEL 076 451 6548 TEL 03 597 5573 FAX 076 451 654 FAX 03 597 5574 E mail support nippongene analysis com E mail feast egenome cs jp fujltsu com URL http nippongene analysis com URL http genome e mp jp Copyright 2014 NIPPON GENE CO LTD All Rights Reserved
14. UV UV 240 260 nm 350 370 nm 320 nm 30 13
15. 1 1 gt i 10 000 ppm 1 ii 70 iv 1 v
16. NE0051 4 7 1 Deer 1 50 ul gt Deer 50 2 1 Deer 3 64C 5 DNA 4 94C 5 DNA 5 Deer 720 0 ul Deer ETT ma Ce I 5 s 1 2 jk EE3 1 Deer Deer
17. Deer B 1 C EDTA D A
18. gt 1 II gt ZZ 1 m J m w Deer Deer 1 I IV 14 5 lt A Deer Deer
19. 48 NE0141 s s 50 Z Jm Deer 20 C 20G GBE30 48 Deer Deer 50 48 48 20 C 6 20 C
20. http nippongene analysis com LAMP LAMP e Genome Order e Genome Order http genome e mpjp i ND NN NN ND Ne ND ND 1 LAMP Loop mediated Isothermal Amplification 2 sus s ua aS u a Sa u NERO OTRO saa NOR RET RR ss a u
21. 160 ul MA t fm L DNA DNA 40 ul 20 0 ul DNA
22. 6 1 160 ul 7 DNA 4 0 ul EUSPAEESEEYEI DBDTEEEE EE DNA 1 Deer i O il PP i 4 0 ul I z 8 20 0 ul 9 64C 30 10 80 CO 2 11 UV e s Deer 0 NT Deer Deer Deer Deer DNA DNA
23. DNA Deer DNA g
24. Nagai K Ishinazaka T Asano M Suzuki M 2011 Sex determination of sika deer Cervus nippon yesoensis using nested PCR from feces collected in the field J Vet Med Sci 13 1611 2 Notomi T Okayama H Masubuchi H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA Nucleic Acids Res 28 12 e63 3 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 7 Deer 4 0 ul 20 0 ul 1 DNA 64 C 30 Deer Deer 4 0 ul 1x 10 Deer Deer Deer 16 e e 2014 5
25. 1 Z ZJ EE TI pil Sika Deer Sexing Kit Version 1 0 0 g NEO0141 ip version 1 0 0 1 LAMP 2 6
26. DNA LAMP Eiken GENOME SITE http loopamp eiken co jp amp amp 1ISO 13485 2003 http wwwnippongenematerial com 2
27. s s us TTF 2 3 ERNE RR ENNIO ORME RR RRR A 3 4 Re RO RR RON eR RN Re RR Rr RR ORO RR ee GRR RNR RNR Re PO RRR RN RN NIG IO Roi RR DCR Meo ORC MRI RDI RR Pe RR Tai 5 5 CSS ms 7 SH ASE IE LCP YPPPIPPPPPPIPYPPPYPPYYU HPPPYP CSS 9 DNA 5 RR OO RO RO OO AO OA Ao A pop oa Roo NON PP 15 6 RI 16 7 NINEGEBEBEGEGREESG 16 Deer LAMP LAMP LAMP 1 LAMP LAMP
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