「KOD SYBR® qPCR Mix」を用いたプロモーター領域
Contents
1. 1 1 1 0 1 1 Olcopy Hf NM copy TEIETTYTTETTTETTTTT bE TTT TTTTTETETTTY ET ET tt TET CWCl Cycle Human actin CpG GC 73 131bp KOD SYBR qPCR Mix PCR Taq PCR Taq A PCR C PCR 4 Log 10 copy 6 Gyela GC Taq DNA polymerase PCR KOD SYBR qPCR _Mix no template control NTC PCR GC2. PCH KDD DNA polymerase PCHR Taq DNA polymerase PCH Taq DNA polymerase PCH KOD SYBR qPCR Mix GC KDD SYBR qPCR Mix GC Taq DNA polymerase PCH kb Taq DNA polymerase PCHRH KOD SYBR qPCR Mix
3. PCHR KOD SYBR qPCR Mix CpG PCR GC CpDG CDG 50 ChIP PCR Taq DNA polymerase PCH GC GC CpG PCR X KOD SYBR qPCR Mix KOD DNA polymerase KOD
4. KOD SYBR qPCR Mix 1mlX1 40 QKD 201T 9 800 KOD SYBR qPCR Mix 50xXROX reference dye 1 67mlx3 200 QKD 20 1 3 000 1 67mlX3 x5 1 000 QKD 20 1X 5 147 000 50xROX reference dye 50I KOD SYBR qPCR Mix 01JKe7 12 2013 101
5. SYBR Green 1 PCR 3 5 KOD exo DNA polymerase KOD 7096 GC PCR GC CDp 1 Homo sapiens telomerase RNA TR gene GC 64 19bp CCCGCCCGAGAGAGTGACTCTCACGAGAGCCGCGAGAGTCAGCTTGGCCAATCCGTGCGGTCGGCGGCCGCTCCCT TTATAAGCCGACTCGCCCGGCAGCGCACCGGGTTGCGGAGGGTGGGCCTGGGAGGGGTGGTGGCCATTTTTTGTCT AACCCTAACTGAGAAGGGCGTAGGCGCCGTGCTTTTGCTCCCCGCGCGCTGTTTTTCTCGCTGACTT Primer 1 CCCGCCCGAGAGAGTGAC 18bp Tm 68 6C Primer AAGTCAGCGAGAAAAACAGC 20bp Tm 61 1C Human 7 actin CpDG GC 73 131bp BCTTCCTI
6. TTGTCCCCAATCTGGGCGCGCGCCGGCGCCCCCTGGCGGCCTAAGGACTCGGCGCGCCGGAAGTGGCCA GGGCGGGGGCGACTTCGGCTCACAGCGCGCCCGGCTATTCTCGCAGCTCACCATG SST GS AGG Primer 1 GCTTCCTTTGTCCCCAATCT 20bp Tm 64 1C Primer 2 CATGGTGAGCTGCGAGAATA 20bp Tm 63 9C KOD SYBR qPCR Mix 50xROX 10pmol ul Primer 1 10pmol ul Primer 2 Human genomic DNA DW total Applied Biosystems StepOnePlus 104 0 4 0 4 1 0 4 1 Xl Yl Opnul PCR 98C 2 min 98C 10 sec 4 60C 10 sec 40 cycles 68C 30 sec Melting Data collection Taq PCR PCR MMKe7 2013 vor101 1 1 1 Homo sapiens telomerase RNA TR gene GC 6426 19bp KOD SYBR qPCR Mix PCR Taq PCR Taq i A PCHR B PCHR 1D
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