科学研究費助成事業 研究成果報告書 - KAKEN
Contents
1. uU gt ES D ooooooo fe ff es ca rs ES L1 L1 C3 E3 E31 EJ SAS aunado Ol E a pd e sn Ee Y II B E E 1 L3 E E3 E3 E3 C3 EJ c4 se E E E E tc ctEBEHg m Drpmir a mimis ui des EET TS EET si NND me ar TE TESI ETE ESE Oh PODO070070 o e lr rng EX epee ES y wy DODI 20000 59 SSeeqo0o05o pjEJEJES S OBS E Esc Sele i A OOO Ono Op OOO 3 n oaoa 8 Hmna mg 28 oogooo E g LricDmuumBug O Ou Cc CL Aru Z E I uS EE nooo 050 E o lt c rp E l c OOgoo ga H rjr uv uumgulcmu jo mon o BOI Gogo E d ESTA E ac cgOoggeggsg oooorF ho Oo as oo g ga 5 DO DO oo Se Se ee gt ji ae E o o0000 soon Bg i E ooygoo Vo oof oooo Bu onooorts o o 200002000 oe E ES f 2 oo r L3 r3 ee SOOO y 80 ES anoa EEJESES wWHINUI ie EE S 200 ripis 0000570707 Hie ee E Ed JEU ENEE T eee 5 iB DOS 2700 Exc e re e HANH Es Oe gu EJ SC Se EJESEJ Ele gg gg Lt rrr Wo LO LJ c oe L m L 1 1 10 O Jo EI Cooo o o A r3 ooun 8 cn aia ear oer temm rem oO Goo pip Sooooe oo ogor eyenuud E fea 5050000000 EX Ea E es See cl e e r3 p Ln Eres H EXE ETES TES ETES E i m O mim ui oO pes para oca Sl o e e ol Ex e mI v oe ee L1r3 Boe Ow p pienmmumnul O Gada A A A A CI CO CICICICICICI LL d AN AP E EL ES EE ES eee d 3 5 8 58 8 8 SEES EL ogoogo Human PRR PRR 10His A xi bep es fe Ren ep EE DES L3 oO oO L3 D oO oO atje eleme e es a eo EX SES RE See Sees ee2. 31 ESF S 66 2 45 116 3 ooo 1 Ed Ed Ed Ed E E EJ ooo OOOO Oe noo Ooo y rr o o E Age me OO 0 Z amp poH N gaw ca CO 0549 oop OOOO SR e OO Peace a OR OOOO q OR i gaa rp L r3 iS AE ooo aa a Cc Pa Ou SA H nooo qe jala L3 Oooo Sie onago Sono oooogoy EJ ooo ooo8a le Ooo 2 r3rc 4n R Ooo um Ooo a 5 Ono Soo Tes O00053 200270 lt lt ogo L3 mcm r3 udi oN O 1 a 3 T Em EXE R 4l Oo cai Er L3 EE m CI 1 1 E E oOo O qum a hae C oo m O o io o 9ae4 4205 0 p EEI aerea cc mg ppm pbHHH HDmrm ao H oa ao ao O a A oy g I oOo ao a ag EZ r4 o L r3 L3 ooo o s N anna aana m naoa ooo Sooo g fy ee ep as er as anf a emf e Fej es EE a G E nooo ae E e ea DCownooo Ej FO ef oooga m EE ES EXP PSLEA ES TES oa fae ac RES pum fn f fun ge os A ODO E ES y aa EE EE 3 E FS Y es ps oe ooog o euot g fs E EST E oo nc oge rr p EFE ES oobo ooooooooon ook L1 L1 L3 E E23 E23 adi A A A Oo y POE OD EOORO L3 r3 Ggagagq qaagad ESTEE Oo 05938000000 E E El P5460 990 90500o0o Ecc cs ss CAREERS S 5p a OpeH Ed MS TEE ESSE EST Es ne e a Es festes rs es ri pp mpm pmpmmnmpmrmimrmintn H e e a EIE ESETEJEE E pri mEmuumHrmR poo a p bx mWBmuagsmmmuimumnuiurm co Lr RE ee nw e E O PQ POD OODODODO DoW OoooooooOoo a a a ea a Seis L3 Fl ooog 3 EXEC a 2 oooo a a E e L3 C ona a Je ad L3 Ld EE ETE mm O e om O o oand 2 mpnmmmm 3
3. 6 20141 0 0 0 Uu A gt WB anti FLAG lt FLAG PRR al o t I B T B T B T BT B T B 45 0 31 0 4 ER PRREPRRE TAO TRE E FH OO fe HT F and Nakagawa T Extracellular domain of the pro renin American Heart Association High Tsukamoto T Aoyama S Ebihara receptor participates in dimerization 2 Suzuki Nakagawa C Nishimura M A Suzuki TER O g 3 E E3 E 0d r36 OOOO ro Soe acess oe ea ooo I SE Seater e e a ae aig GNE aie El pg ESL ie San ESEEST E53 ao Bee o a Se e Pelo o seas o e GB r e cs IS e PO E Es Y a cs JA ma o AE E a Uc ES EE Giotto ooo o e EE ooo oo Es oO a Pressure te fo pal Poe e E E ps Y T Dr Ca O Uu rr Blood EE EE Es Ez Ey I Nog pe oog 492 7 Ea Pay ae a pen Ea CI os eu p4 H a o a naO toa o cacacac c Homme 0000A uuu s ro ee e ARAS Sea ES ESENENEETIE SaaS ooo oM opoooooooooOo e CICICICICICICICICICICICICICICICI 9 Lp T ttp www abios gifu u ac jp hnakagawa HOU HOU h 9008001081010 NAKAGAWA TSUTOMU 0281049 UU 1
4. E je See ea Ex aS Sere dE r1 ppmpm ao ao amp gcpmpmmn oo rj eo zoo oo o jab ae Et EX SSS e cn IIA a a Exp base ooo exire EL EST GC d d a A ad aA A A ES A A A A A A A CJ O O O a A A A ge S ia ooooaoo aa ooonaoaoo nona nooWooo oo E ESL ele L EX e e eee EE perro og nm3dum poa Hrpa ooo oF OOF cc o a a lo e o o e e o o e o y 222000000 co y HHimuumuuug 7 DODODODOE ODO ondaa ees fem ef a a E e M Hattori Ebihara A Suzuki F and Nakagawa retention of the Iwata H Intracellular extracellular domain of the pro renin Noda M Suzuki Nakagawa C Nishimura M Ls HOUOUUWUOUU i A Y a Y ES Boo LI rs PL Y 2457200 Biotechnol Biochem 2014 in press cane HE 2 Suzuki Nakagawa C Nishimura M receptor in mammalian cells Biosci KE EC a ia fc a Y e O Me ie ES B ODO Eg de e EE EP EI Tr ES zu Er LcgdpJ LcmME O LJ LL LL L3 LL Tsukamoto T Aoyama S Ebihara Om L3 9 o Y e Y Boop ooo Poof oooO oO Bgenmnuuug peru Oooo Sl Egg iE e O d DODODOSO a E n Y pea L3EEEB E nno9 pipa pa c3 O 0 F and Nakagawa T A Suzuki Participation GST GST GST GST GST of the extracellular M8 9 ECDd4 _ KDEL GST ECD B T B T B T B T B T B FL U m 22 D D ui C gt C O Ke m D os c c aA Vv oc 28 C 6 c o c N mes c 5E 5 O WB anti GST S a Commun 444 461 46
5. ae ees UA EI EN LL ELB EL ELO LT ET D LU EU TL BLO E HU OU 0 UU unu aaa a ada OHOOOU opo00 0 00000 2010 2013 Aa aa AA ugumggug gtladenti fi cati on of a novel bi ndi ng protein for the pro reni n receptor IA OO HI Nakagava Tsut ono ao a en HI DRG TNR AE HE ETH REL E P HI EE E IRI P ls 3 400 0001 1L L1 D D LI L 1 020 000 L IL 1 L IL 1 L IL 1 Col Col L IL 1 O O O O OOO L JL E ugugagduugudg dut The pro reni n receptor PRR is a single transnenbrane receptor for the pressor e nzyne reni n and its i nacti ve precursor proreni n PRR induces the catalytic acti vati on of proreni n al ong w th intracellular si gna ing In this study the gl utathi one S transf erase f used extracel ul ar donai n of PRR expressed i n nanmal i an cel s was observed by i nmunof uorescence nh croscopy to be local i zed i n the endopl a smc reti cul uml i ke nenbranous structure and recovered i n the detergent phase i n detergent based tvo phas e separati on experi nents suggesting retenti on i nsi de the cell through stable nenbrane associ ation By co Mast pais of e PRR wth its interacting proteins i n hunan cultured cell s and nass spec tronetry 78 BP was identified as a novel binding protein for PRR ugaggul p8g0000000 00000000006 000000 dde eee VpD 400p0 00000 00000 00 UNUUUOUUOOE O 0 UYU O d000 d0000 D0000 00000L Uu r1 QUO mnooooo nooo mofo lose b
6. c 00 lc cc a 4 no 5000007000000 O ric c 9500007000000 070 os oa e a e o Ss a o Y e o e a gc e o E pa a fe a a E cart ps co 0 e cs a a gesna o IT 3 C3 C3 5 gag amp 9 ye 9 LA SO oP Cell lysate Culture supernatant N kDa Y S 49 c kDa YY 116 3 p 66 2 FL Ecp 45 KDEL GsT 21 5 A 116 3 66 2 45 31 21 5 I I A B BG ggouluiuu ermtubtutltutt IFT SGSTRRA 47 280 23h E 2 cos 7 Bh8l oo soo O0 HA CIO Uo 1 r3 4 4 r3 L 2 I3 EXP ces a e a oooooooo 3 poo00000 m m m m Fn m goong p EJ oaa adi ga Se Ei L L3 9 C3 C3 FI C3 E3 EJ Co oot ooo ertootooo oooooo 3 E3 EJ EJ E3 E DJ 3 E3 EJ E3 E3 E31 DJ 8 DODOO poorno 1 L3 L1 Oo CI ooo nonno GOHO a or EXETETE LIES ESEJIEXAE r3ccmmnrur gar A e e Y a noe Poo oo EE E ac noo See ee 1 EC C3 E3 EJ EJ EJ Ege EF ee ooooooo MAnnn0o00 B Cell lysate 9 d Da v P 49 116 3 SEIS c gt o a 5b vo o Ay a 3 9 kDa Y e 116 3 3 L1 E3 E3 ca E EJ 3 C3 E3 E3 EJ E EJ oooooo oOooooo u Oooo oOooooFf n SS See E 09900400 pe PA es e EIE C dQmBHHBmHHBH C o d L3 p3 L2 A r pmpmrirmnrriri c3 Hmprmmi ooo p Mg B hmH ooo L3 A oHa ooo 208 of oog gA D Oooo nOFoooooo 3 L3 E3 EJ E3 E E EJ C3r3E3E3EJE3E3 n FL f Ecp lt e o kDa Y F 116 3
7. e i aa a eae aa Cc cao l Eon cud 0 ES 3 F3 c3 H 3 C3 S ecu ooo2o0og Hoon pa mg Boo soooeo Hoog Ec E oroo o e a pz t EE rr ES eee ee neon Pe ei a 20m pj a e ES a EE ES ee a c HE ooog A H la nog uy sc a U ESI ES OP ce Eo L3 rc goo Oda E s RU CICICI pn Cc a ang Cobo poto go H rm rz Be S pu ooOonoonss 8 EUG EX EET ooo Y o 42 ooooro OOOO nooooog sotia o g ar Ey Ies EE ES PEERS E qeononoon o8ofo05 eae SSeS Se pEXEEI ost Exp E oocog L3 Siete AC WETIGDIESRES Cc CN LI ooog ond d UC d a A ad ad ad Ey ad E EST A EI a eea muimiimiumnmiuumiumunri so e e E o ooononocoooooog Ha tf moe e o cect o A e e we ct e o fmf ve ooo ooponnpn00 pe EE E ESTE rn oe ES Eit EI EET EIE Jr ce a ES ES ESLESHEEREST EHE ES SESS E35 Eder EVE ES S ESL ESL ESIESISE ESL ESL ESI ESTE ee Se Eder ao C E o o ESTEE EST ESEEST EST 00505900 EI e cc o mn Y e ea x E Ed fend E3165 3 riemagubdda m c 20102 SaaS St eS a a riccauteraumauadggmamiaumumugmumuuumuummumimd qe uuuumumuiduuigmi ouuuiudmnmum pguugpgoouog 507050 S COIEJE ELIBEU OD 5D000000g d Oo 3 rjHaIOgntgou usmuuunmuumuuumuuuuu Es Per ELE a ES Ed SO POS 0000 O anama AARG RA st ee ff ef tea 8 200000000 OOOO OOOO fi fl ey po E par ES E ET Ep nt Es ft Spree ES ne fl EST E EE A e E Sa e o o ES o e ESEES ES os e ES ps a ca e ca a mee ca Y e O ma ca E a Y pa ca a Y ns a AI a
8. ee ee ES la ES 3 cs e a pr ES Cj DO 4 OOOO O SR OO a fc a e a 0 OOO 52 oooO ta E 55909509 0500002000 i e e Een a C a ES COPOS aes L3 r4 C3 EJ cp 2o00 OSO S ae ele ye NA OOO os poo oO Oeonoong ia ere oe yg o soo y 2099270 058075 pc JU mnoeoo Seen deca 58 oh ooo LES b GO goo of DoS oO 0590000 O 4k ooRoOooOoF oo gA nonn aS 3 Serer nono xe ie aA EXE ES Cc o a e o om e me e a gm e EE EST S ET ESTE ie 08 Soy Bug opos Ex m e aj SEES o e ET e e ol eee SaaS o00 570g Nooo Oo 52050092 Pay ai aie St ee eS ers Sei ar SS ee era Oo c o H oao ca Ei ASE okon Ogogo ooon oo oo Seas og gaq qa qaa ag atft nanoa e Y e a Y e e LEE Y ee us Y re Y pa O 3 3 par E oe Ege 66875009 500 se Ga CA O e n r e e E Soo cs o cs ES Es e o aon me eo Sy 7 22000 EX e a re E co gu metas SOREXENPUES IEEE EE O c HEELT eti mm ooo pa mg ODO oooO goo a e e e fs E EL ES Y es Sal ono oFooooYs ap ap 26000280 a cc ES Y e ce O rs o e Ts oopan ooo of 1050 ooo ooo mnmmniuuiniummmnmpnunmim HL EXE es eS Slee Lee fea Y oo ooo a e a CiM s e t O pnuumpuuuudgjg OZ gt oeo poh PODOADODOOOO DODODOOO AE i9 ooo poro Boog o ee et fe 9 E ps 0 ms a me ae em cm am ES Locos postas 0 pecas 9 Ormnmoagg rourbrbmurmrprrEmrpr a oe a ee a EST rs ES So ooo a oe e L es ce ef ET LriEdpECEIEIEJEIEIJEIEIJEIEIETJEdIEd r3 ric cs cs cs cs e cs cs cs cs g Soyo 09 oooO couuagunuuuibVrm5 rnuumuumunmimnug EIE EXISPERESMES RES ces e a E a JO cs s
9. ooo asf es e e ez Exc Eel Ei6nm7788 f 3 ooo oo Sa o re eo oOoofoo mh rina S Pop OOO E S n oe ee Y ODO duum o2 Ee 20000 Wooo 05208003850 ea a Sge ee E ES aa E L3 EX E EIE SO m Au a PE L3 Ex SURE EXE ae Ho a a ee ee a Sl e AE PFO a QuOoZO ga umm L3 EI aa omg Ao oc Pie ES SER Aia l a sE SUE ES L3 oP ooooo Z oE ty E oe E numus o ooMooooo m Eg oo oD na DO mnuuHmibnuugnu mm umntuguru CL AmnmpmumnHrpmg rmuumru Ex eter Seer A DODOON oO Siete eS a EJ es ee ee ee es C3 x OR OZOOY OOO o e Y a 0 Y en e e e e ooo dy a OH c m esc fa oe en m as OMA ce e yet y en ms ESL ESSI Agra o oof onooYt oosoooo a rc oooooo cce marta e GOmnumnumnuuunuuaug E a O L3 r3 dejo l gt l Ei ES SS Ege 2 ej ele n EE ase ae loe y Y DODODOODOO OOOO OOO DOS OOO HE E i r A G529550 DOOS00507 004007 Hogg a aS a oe a a E ele SE a a L3 r3 PE ooo Poor a oops Es A4 9 Po ogog0 PO 00 oOo og co E E L3 CI E L3 Bs m EIE r4 Sal O gt 50000 aes a p us um pd um O7 O Sono B c pe pel A a e e e e aa a cum m o e o a EE Eee See p EE a re pe E miei qe ESI ES pies es Ed ESL p a a Ec e noo Ej Sees L3 03 SOF cUm rnm zz 1 f ooog 0050000 o 0000 ooo QU r3 pb g H p os e a A a o as EX e a a A e os ES bes Ee cr Ei Di oooO L a oo o aoa a EE E ooa O aoa A a A aG a E 53 O auugagHngng S 2 no00000 0 3 88 ono oo oooe em Ee ME o te en Ms ES B2 00000507 DOCOODOOD ODO DAA As oniinn g ON cm a a oat hie a eis ea
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