KrosFlo Research IIi タンジェンシャルフローろ過システム
Contents
1. 22. 8 9 KF Comm 4 1 1
3. 10 4 2
4. DONC NE A 8 2 J MasterFlex L S ACR2 H3S 01N ES 13 14 16 25 17 18 3 MasterFlex
5. 1
6. 20 9 2 9 2 1 KF COMM KrosFlo Research Li Windows MicrosoftY Windows 95 Windows Microsoft Excel 2003 Excel ActiveX control COM CD
7. Spectrum Laboratories Inc
8. 10 1 10 1 1 26 ttt Dead End Filtration TMP PTMp P 10 1 2 TFP
9. KrosFlo Research Hi NW WW x MIN MN ise Tubing US 13 14 16 25 17 1B 8 1 WN FDA H
10. Rejection 1 C BE C a U01 90 rejection 30 rejacton N 20 rejpcton 9 rejaclion 0 2 15 Web http www spectrumlabs com lit hfdial pdf 10 5 2
11. 4 1 KrosFlo Research Ii 2
12. 0 5psi mm 1 10 3 2 0 5psi min MC LC 37 10 4 KrosFlo Research Iii 10 4 1
13. TMP Shear Rate Shear Rate 8 Flow Rate 10 2 KrosEFlo Research Ii TFF 11
14. W v 6 FDA 0 1 600 rpm 180 oz in 13 kg cm 540 oz in Line 0 1 F S Load 0 1 F S Drift 0 1 F S 128X64 LCD LED 5 0 45 0 psi 34 5 310 3 kPa 90 260 Vrms 50 60 Hz Universal Input 2 2A 115 Vrms 1 1A 230 Vrms X
15. KF Comm EE 12000s 40005 KF Comm Trial O Flux VT Q ENA A EE NR M J
16. TMP Prup PA Pap 2 P TFFE WPM 27
17. LCD ooo 4 Tl ER 7 2 3 Spectrum Labs G0 TT 210 ml min psi mbar psii mbar psi mbar TMP
18. Flux Flux 0 ECS
19. Windows a b Research Hi Scale_ 2 1 c Computer USB USB OFF USB USB d USB USB KrosFlo Research Ti Windows
20. 10 3 10 000 2 Document 400 12058 000 KrosFlo Research Li
21. 10 3 E Irial Data O 25 10 0 H 03 Dagteg 732010 Location Specirur Labs App Fiber Court Moduke 5 5 HS EHU U2N Application Concesntrate D afilter veus Fiber 1D Serial 3237572 Objective 10 Concenfration 46 DY Wasn Her Lengith Surface Area 29 sq cm Attendees Feed Rata 4000s ubing Size 35B Notes Feed Rate 12000s t pumn Calibratinn Foz calc d from a manual value manual walug Ave Permeate Flpt 102 7639 LN a Ave Permeate Flow 40 672 ByWeight fMndulg Table Date Domperny Dur walued Starting Amount S50 _ 1 4 1 101 8 1 1L 4 2D 1D1 B 2 1 HH 8 Ole Me By Volume
22. CF VRF V V CF VRE V V 10 4 3 20 100 2
23. ROM HW 1 FIQ ram Aa CPU HW 2
24. 3 10 3 2 KF Comm Integrity Test psi min 5psi 0 3 bars 10psig 0 6bars 5psi 0 5 psi min 0 5psi min
25. KrosFlo Research Li Hf EN 61010 1
26. Silent Alarm C Tubing Calibration Tubing Calibration Volume 1 4 QO QO ml Exit lt lt e 210 mmin W 1 gt CO DO 10 2 1g 1m0 ON SETUP TUBING CALIBRATE
27. 1 Configure 22 Collection Configure Collection TFF Trial Template Excel 1
28. SYR2 U20 01N MicroKros Midi Sampler 2 30 min 110V 220V Pharmapure 14 16 ACPC F16 01N 13 14 16 ACPC F17 01N 17 ACR2 SC4 01N 4kg 110V ACR2 SC2 01N 4kg 220V KrosFlo Research Li 1 1 1 1 USB 1 1 3 KF Comm 1 KrosFlo Research Ii 1 1 Pharmapure 14 7 62m Pharmapure 16 7 62m 1 IN 2 2
29. TUBING CALIBRATE 9 G 9 H 9 Feed
30. 30 5 8 5 0 1 VM 5 6 7 5 5
31. 8 4 A 1 4 1 2 1 2 3 30 30 4 3 4
32. 14 13 4 4 Luer 5 LuerXHB 07 6 7 8 9 10 2 2 KrosFlo RESEARCHTi 6 15m0 1 32 HB 2
33. 11B 4 2 28 11B 5 MiniKros Sampler MiniKros MidiKros MicroKros 12 13 14 3 13 14 MicroKros MidiKros 1 16 Hose Barb
34. 8 7 KrosFlo Research Li 70 9 KF Comm 9 1 VERSION 1 4 _ J 4 1 3 a FOR USE WITH THE KrosFlo Digital PreSSurellMomitoi LABS COM Copyright 2010 Spectrum Laboratories Inc Rancho Dominguez USA All rights reserved Spectrum Laboratories Inc Rancho Dominguez USA CD
35. 3 90V 4 PS 40 5 ZN 13
36. Pro Connex T H1 6 500m0 1 8 HB OO 0 NN Ol DO ACPM 799 01N Pro Connex TCXHB XLuer 16 17 500m0 4 ACBT 500 C1N
37. L S 189LS 1 9 16 L S 25 L S 17 L S 18 amn 2 2 2kg 2 6 6 E 2
38. KF Comm Module Characteristics NWP TMP Qomeae NWP 10 3 3 1 2
39. 4 Spectrum Laboratories KR lli System Pressure Calibrate Alarm Settings Tubing Calibrate Hardware Setup DTelalelsiilers Exit A Pressure Calibrate ACPM 499 03N or ACPM 799 01N Feed Permeate Retentate FEED Pf Set Clear
40. TMP Flux 1 2 Me TMP 100 Flux TN Flux TMP Flux KF Pe TMP 2
41. 40 14 10 5 2 2 3 10 2 1 10 3 TMP 6 30psi
42. Windows CD FTDI USB Drivers Web http www ftdichip com Drivers VCP htm FT2232 Windows FTDI USB Drivers 9 3 9 3 1 TFF Trial Template Excel KrosFlo Research ILi System Manual Override Bxcel KMP System Excel
43. RE OR 50 1mm 10 7 TMP Flux 2 TMP 2
44. TMP 10 8 FLUX 12000s 4000s
45. m0 min rpm 4 7 2 4 13 14 16 25 HP15 17 HP24 18 HP35 36 HP36
46. c d 6 e f By Weight PSI mbar i 40005 12000s
47. 500m0 ACBT 500 C1N 1 4 6 12 13 14 6 HB X HB 12 13 14 5 11A 29 11B 4 T
48. COM yes 1 Windows USB USB USB KrosFlo Research IIi KrosFlo Research Ii TFF KrosFlo TMP 49 11 2 1 1
49. AP QA IE TMP TMPF SP EP TMP TMP Flux 16 0 42 Flux Limhr TRANSMEMBRANE PRESSURE bars TMP bars 16 Flux TMP 1 TMP i TMP Flux TMP Flux TMP h
50. KrosFlo Research Il i 39 Batch Constant Volume 10 5 1 2 9 10 11 1 13 10 2 2 10 3
51. j Flux k C Module Characteristics NWP 24 Time H MM SS a lee Oe TMP DP Qinet rpm aaneate Temp TCF Water Flux 1m 1 m h Water Flux 20C Water Flux WP NWP
52. 1 2 3 4 5 1 4A 2 260V
53. Configure Scale 48 11 1 4 11 2 COM 1 COM Pressure Monitor Override Scale Override Excell CD COM COM
54. End Collection TFF Trial Template 3 CF and Fresures Ys VT FRat ys Tme Fux CF FesdFow Ratg ws Tme FrEssures ys EE Fornndas Integrity Test Module Characteristics FLUX Trial Data NWP Module Characteristics E LUX amp CF and Pressures vs VT Flux
55. 9 3 2 TFF Trial Template 9 3 3 KrosFlo Research Li 1 CNow 1 COM 4 Data Bxcel
56. TMP 0 5bar 2bars 30psig Flux TMP a Flux 3 4 Flux 43 TMP TMP TMP 2 3
57. CLEAR 5 10 4 4 1 vol rev 10 8 10 60 m6 min cal volume m6 13 0 006 36 0 m6 min 4 1m0 min 60 1 m0 min 6 m0 360 360 4
58. Override KrosFlo Research Ii USB COM TFF Trial Template COM KMP System KrosFlo MiniKros Pilot TFF Excel Excel 8 A 21
59. 8 KrosFlo Research Li 8 CS 7 2 IEC LCD EN 61010 1 A
60. A 1 5 95 99 1 5 4 4 11 Ni Potontate Retentate 0 EEE EE Hollow Fiber tht 1 oR thy 1 Tangential Flow Filtration Filtrate
61. Microsoft Windows Excel Microsoft Corporation C Flex Pharmapure Saint Gobain MasterFlex Thermo Fisher Scientific 1 J 2 3 3 4 4 5 4 1 KrosFlo Research Ii 5 4 2 KrosFlo Research IIi 5 4 3 KrosFlo Research Ii 5 4 4 4 5 6 5 6 6 7 KrosFlo Research Li 7 7 1 7 7 2 8 2 8 2 2
62. 3 EE about License Find Comm Period BU Secands per line en 17 COM OK COM COM Find Comm OK NOW IIHHUIB lable Uale Scale Setup Comm Port about License Find Comm Scale Accessory for KrosFlo Fiesearch ll TFF 5ustem Scout Fro Cancel 18 2 Microsoft Offce 2007 2 Add Ins Configure Pressure Monitor Find Comm A o
63. 60 1 3 10 000 Excel 65 000 1 18 USB COM COM Find Comm KrosFlo Research Ii USB COM 1
64. 30 lt 70 36 9 3 5psi 10 30 1cm 2m0 2m0 cm
65. NN 0 7 MiniKros Sampler MidiKros MicroKros 12 13 14 MiniKros Sampler 12 1 17 1 4 ACTU P17 25N 5 7 1 4 HBx 1 4 HB 500m0 ACBT 500 C1N 250m0 500m0 1 8 HB Xx 1 4 HB 7 Pro Conmex BX TCXLuer
66. 3 lt Flux 1 Start Collection Flux Qsmeae TMP NWP NWP TMP D AP Integrity Test psi min 0 5psi min
67. 7 8 6 2 1 KrosFlo Research Ii 2 8 9 10 2 1 8 4 2 3 1 8 1 2 1 1 8 4 2 2 9 10 2 1 9 2 A gt
68. KrosFlo Research Ii
69. TMP TMP Flux 10 8 Flux 3 3 10 8 1 1 10 8 2
70. 1 8 8 9 HB MicroKros 14 1 MicroKros Luer 3 1 1m0 1 16 T 1 16 HB XLuer 2 15m90 5 1 16 HBXHB LuerX1 16 HB 2 14 13 50m0 ACBT 050 C1N 14 3
71. GO 3 INC DEC 73 9 R PeTmrealc 9 MP Ccncentration acto 12 KrosFlo Research HI1 SYR2 U20 01N KrosFlo Research Ii KF Comm ACPM 201 01N KrosFlo 110V KF Comm 3 RS232 ACPM 202 01N KrosFlo 220V KF Comm 3 RS232 ACPM 499 03N 3 ACPM 799 01N 1 ACPM 799 01S 1 ACPX KT1 01N Pro Connex 3 4 TCX 1 4
72. Flux 90 6 m h 45 0 75 m 1 5 9 0 75 h 90 9 m h 0 022 m MiniKros Sampler Plus M52M 260 01N 460 cm 1 50 0 36 22 45 11 1 1 1 KrosFlo Research Ii 11 1 1 KrosFlo Research Li KrosFlo Research li Research Ti USB Bxcel KF Comm 11 1 2 Computer USB
73. Cal Quit B Spectrum Laboratories Pressure Alarms Pf Hi Stop 22 0 Psi Pf Hi Alarm 10 0 Ps Pf Lo Alarm EFRS Pp Lo Alarm OFF Psi Pp Lo Stop OFF Psi Silent Alarm Exit Alarm Settings OFF PfHiStop Pf Hi Alarm Pf Lo Alarm Pp Lo Alarm Pp Lo Stop
74. 10 8 3 1 i 0 7bars ii 4 9 10 9
75. LMH 1m 1 0 m h VT cm Pump rpm Temp Conc Factor Shear s LMH VT 9 3 4 KF Comm 21 CFR Part 11 077 578 0166
76. 10 4 10 2 1 12 13 14 MiniKros Sampler MidiKros MicroKros 1 KrosFlo Research Li 2 11A 11B 2 2 11B 3
77. PS mPBS ME 5 0 1050 cm 0 5 1 0mm 10kD 0 5 um 8 KrosFlo Research Li a BE 4 Research Ii
78. KrosFlo Research Li 1 Flux Flux KrosFlo 10
79. 38 lt H AH I 10 4 4 e 2 718 4 e 1 50 98
80. 38 10 4 2 38 10 4 3 38 10 4 4 39 10 5 40 10 5 1 40 10 5 2 41 10 6 42 10 7 42 10 8 44 10 8 1 44 09 2 44 10 8 3 44 10 9 45 11 46 11 1 46 11 2 49 12 51 13 52 KrosFlo Research Li THFF KrosFlo KrosFlo Research ii TFF KrosFlo Research Ii
81. 5 16 3 8 5 32 1 4 2 15m0 C Flex 3 2 1 1 50m0 C Flex 3 2 1 1 250m9 C Flex 3 2 1 1 KrosFlo RESEARCH Ti 6 KrosFlo Research Li C Flex Pharmapure 3 ME mPES PS 99202 7 KrosFlo Research Hi 7 1
82. RS232 RS232 KrosFlo Research Li Valve RS232 RS232 Computer USB A A CPC F16 01N 1 4 maxy 0 5A 12V CPC F17 01N 5 8 maxy 1 0A 12V 11 2 2 TMP 1
83. calibration volume Prime START CAL VOLUME CAL VOLUME 11 C m0 min m0 oz min oz 11
84. hose barb 1 4 HB Xx 1 4 HB XLuer T Luer 2 500m0 1 1 4 30 3 4 5 6 7 8 9 Luer HB Luer HB 1 4 HB 1 4 HB X Luer Luer TT 9
85. 600rpm 2 40 MM 4 7 2 4
86. C FLEX Viton ee os f a MP 8 3 KrosFlo Research Ii KrosFlo Research Li ACR2 H3S 01N 0 7 KrosFlo Research Li 1
87. 0 01 2300m0 mm KrosFlo ResearchTTi TFF KF Comm TMP MS Bxcel 1m9 KrosFlo Research Li
88. 3 13 17 MicroKros 1 m0 50 m0 MidiKros 5 m2 200 m0 MiniKros Sampler 50 m0 40 3 15 50 250m0 14 16 10 2 4 5 EE KrosFlo Research Li
89. L S lrpm 600rpm 600rpm 600rpm m0 m0 min psig bar psig bar in mm Hg ft m H 0 LZS 13 0 06 36 IO 40 2 7 26 660 29 8 8 L S 14 0 22 130 25 1 7 40 2 7 26 660 29 8 8 L S 16 0 8 480 25 7 40 2 7 26 660 29 8 8 L S 25 1 7 1000 20 1 4 35 2 4 26 660 29 8 8 L S 17 2 8 1700 15 1 0 20 1 4 20 510 22 6 7 L S 18 3 8 2300 10 0 7 15 1 0 20 510 22 6 7 3 rpm 600 400 oz in 29 kg cm 180 oz in 13 kg cm PP PPS PA FATA SY i NR 0 40 C 45 gt 65C 10 90 15 2000 m WXH XD 8 8 cm X12 1 cmX7 8 cm 8 8 cmX14 4 cm 7 8 cm 0 5 kg NORPRENE PHARMED TYGON
90. CD CD Setup exe Setup exe 9 1 C Program Files Spectrum Laboratories KF Comm control windows C Windows System 9 2 2 USB USB Computer USB
91. HB X Luer 1 mm ACPU 021 01N ACPU 022 01N ACKR2 H3S 01N ACPC F16 01N ACPC F17 01N ACPC F82 01N ACR2 SC4 01N ACR2 SC2 01N ACR2 SKT 0OI1N ACR2 UFP 01N ACBT 015 CIN ACB 050 C1N ACB1 250 1N ACB 500 C1N ACTO0 2PP 01N ACTO0 2PC 01N ACTU E13 25N ACTU P14 25N ACTU P16 25N ACTU P25 25N ACTU P17 25N ACTU P18 25N KrosFlo 2 36 min 110V KrosFlo 2 36 min 220V KrosFlo Research II Ne 3 KrosFlo 1 8 1 4 L S 13 14 16 110V KrosFlo 3 16 5 8 L S 25 17 18 15 24 35 36 IP 26 73 110V KrosFlo 0 75 1 0 IP 82 70 88 89 110V KrosFlo Research li USB 110V KrosFlo Research Ii USB 220V KrosFlo Research I i L S 13 L S 17 15m
92. 10 3 1 6 10 3 2 5psi 0 5 psi min 0 5 psi min
93. 267 X203 X203 mm 5 9 kg Enclosure Rating IP 33 per IEC 60529 4 0 40C 25 65C 10 90 2000m Pollution Degree 2 ABS ACPM 499 03N ACPM 799 01N BT UL 61010 1 CAN CSA C22 2 No 61010 1 CE EN61010 1 EU Low Voltage Directive EN61326 EU EMC Directive RoHS 4 1 KrosFlo ResearchTi KrosFlo Research Ii PLC 10 3 1 32 18 3 8 0 01m0 min 2 30 min 4 2 KrosFlo ResearchTi KrosFl
94. 8 2 0 9 7 2 4 9 A 10 B 10 C 11 2 2 12 2 0 NZ 13 8 KrosFlo Research Ii 14 8 1 14 8 2 15 8 3 16 8 4 17 8 5 17 8 6 2 18 8 7 19 9 KF Comm 20 91 20 9 2 21 9 3 21 9 3 1 41 0909 22 UE CR NE CE 29 0 2 26 10 26 10 1 26 10 1 1 26 10 12 IF 27 10 2 28 10 2 1 28 1022 36 10 3 36 091 36 10 3 2 36 10 3 3 37 10 4 38 10 4 1
95. ACR2 SC4 01N 12 MiniKros Sampler OO 0 NN OO Ol DO ACPM 799 01N Luer X1 8 Hose Barb 14 16 250m0 3 ACBT 250 C1N ACR2 SC4 01N 13 MidiKros 33 OO OO NN 1 RT ACPM 799 01N Luer 1 16 Hose Barb V TF 13 14 15m9 3 ACBT 015 C1N IIR ACR2 SC4 01N 13 MicroKros 34 MidiKros 13 1 Luer MidiKros 2X 3X OO 1 13 MidiKros 1 3X 2 3X 1X 1 8 1 i Ce
96. ESEARCH li FF SYSTEM KrosFlo Researchll7 IS CERTIFIED Spectrum KrosFlo Research ITi Spectrum KrosFlo MicroKros MidiKros MiniKros
97. TMP TMP OPEN TMP INC DEC GO TMP a GO GO GO 3 GO b KrosFlo Researc
98. 16 MidiKros 1 8 Hose Barb 17 MiniKros Sampler MiniKros 1 4 Hose Barb 15 50 250m0 3 3 15m0 1 50m9 13 MicroKros 2 50m90 5 1000m0 14 MidiKros 2 250m0 30m0 100 16 17 1 8 1 4 hose barb MiniKros 2
99. Luer 2 250m9 5 1 8 HB XHB LuerX1 8 HB 2 16 50m0 ACBT 050 C1N 14 3 16 4 4 Luer 5 LuerXHB 6 7 6 250m0
100. 30 Flux Flux 15 30 Flux n 8 h Flux 9 m h 1 1 50
101. Rate vs Time Trial Data Flux vs CF Feed Flow Rate vs Time Pressures vs Time 23 B Date 1520 Location Soectrum Labs App Fiber Count Module Xx3 5003 500 02N Application Concentrate Dlafliter VIrus Fiber 1D Senal 3237572 Objective 10X Carcentration 5 DV Wasn Fiber Length 1 c d frorm a rnarusl velue Surface Area gH A end eps Feed Rate 4000s 3 rmi manus l value Tublnq Slze 25 Notes Feed Rate im 12000s Pump Calibratioan 1 Membrane Type Company Our v Uste me Rating Starting Amount i 5 PsE Emb Ave Parmaate Flux 7E M Ave Permeate Flow mmin Byweight CO By Volume Modulse Taole Date a b KEF Comm
102. 0 10 3 2 6 10 6 AKI 50 Na
103. 46 CD FTDI USB Drivers http www ftdichip com Drivers VCP htm FT2232 Windows FTDI USB Drivers Windows Windows 2 Windows e USB 11 1 3 KF Comm VERSION 1 2 DATA COLLECTION SOFTWARE KrosFlo Research Illi
104. 9 3 50m0 3 250m9 3 500m6b 4 20 20 Pharmapure L S 13 1 32 7 6m Pharmapure L S 14 1 16 7 6m Pharmapure L S 16 1 8 7 6m Pharmapure L S 25 3 16 7 6m Pharmapure L S 17 1 4 7 6m Pharmapure L S 18 3 8 7 6m MBT Spectrum Labs Japan 520 3015 2 6 8 5F 077 552 7820 FAX 077 552 7826 emall Spectrum jD spectrumlabs com web www spectrumlabs jp 2
105. D Hardware Setup Spectrum Laboratories Setup Menu Auto Tare Pressure units Ps Time 19 17 10 Date 03 08 10 Key Beep off Display Constrast 12 Psi Decimals 1 Key Timeout Yes Runnning Change off Exit Auto Tare ON OFF Pressure Units psi mbar Time Date Key Beep ON OFF Display Contrast Psi Decimals psi Key Timeout Yes No Yes 10 1 2 Running Change ON OFF E Diagnostics 7 2 5 a
106. h Ti GO SLO 1 Permeate OPEN INC DEC S50 3 GO
107. iRermeateiSeale MM EABSCOM a KF Comm KrosFlo Research Hi Trial Data 9 b COM COM Exce 1 Microsoft Offce 97 2003 Data menu Configure Pressure Monitor Configure Scale options 47 COM Find Comm
108. o Research Li MicroKros MidiKros MiniKros Sampler Sampler Plus MiniKros 15m 9 20 4 3 KrosFlo ResearchHi 3 USB RS232 2 4 4
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