取扱説明書 - 東洋紡ライフサイエンス事業部
Contents
1. 2 PCR Namberg cyces 94C 2min 94 C 2 Denaturation 30sec Primer Tm 5 C 55 60 C 30sec 68C 72C 72 C 1min kb PCR target 1min 1min kb PCR target 3 Annealing 5 1 Denaturation time 94 25 30sec 98 C 510sec 2 6kb PCR Tm 707C 3 10kb dNTPs 0 3 0 4mM 4 Blend Taq PCR TAcloning vector 1kb PCR 2 gt TOYOBO af w 9 002. PCR PCR Blend Tad PCR Blend Tad KOD Dash PCR Blend Tag gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio 7 lt PCR gt 250Ux1 BTQ 201 Blend Taq Plus 250 Ux1 x 5 BTQ 201X5 Blend Tad Blend Taq Plus Buffer BTQ 1B cDNA gt lt ReverTra Ace KC gt 100 ESK 101 ReverTra Ace C lt TA gt 10 TAK 101 TArget Clone lt
3. Ta gt 100 lx1 TCP 101 anti Taq high 100 lx1 x5 TCP 101X5 lt PCR gt 200 NPK 601 MagExtractor PCR amp Gel Clean up ao MK dNTPs Mixture 2mM NTP 201 8 1 Barnes W M Proc Natl Acad SO USA 91 2216 2220 1994 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifesclence toyobO jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order lifescience toyobo jp gt TOYOBO TEL 06 6348 3888 FAX 06 6348 3833 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
4. Mg 2mM 2 gt gt 3 Blend Taq human genome globin 17 5kb gt TOYOBO af 8 9 00 lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobO jp A K URL http Www toyobo co jp bio ON 4 PCR 1 PCR Components Volume _ Final Concentration 10x PCR Buffer for Blend Iad 5 ul 1x Blend Tag 2 5U D 0 5 ul 125U reaction 1 50 ng Plasmd 10 1000 ng _ Genome 10M 1 0 2M each 2mM dNTPs 5 ul 0 2mM Autoclaved distlled water to 50 gl
5. lt 12 00 13 00 lt 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio 6 human globin 3 6kb Blend Tag A human globin 3 6Kb 1 Blend Taq A Blend Taq 5ng A Lane1 0nghuman genome DNA Lane2 5nghuman genome DNA Lane3 10ng human genome DNA Lane 4 20ng human genome DNA Lane5 40ng human genome DNA http www toyobo co jp bio Long PCR PC12 RT PCR PCR ES RT PCR PCR PCR Tail PCR Blend Taq
6. 14 03 Code No BTQ 101 Code No BI 101X5 Code No BIQ 101X10 20C 1ag Blend Taa 5 Proof reading Taq DNA polymerase pol I Proof reading ow Blend Long PCR Blend Taq PCR Taq DNA polymerase w Blend DNA PCR TaqDNApolymerase PCR Taq DNAPolymerase PCR 1 Blend Tad 3 BTQ 101 BTQ 101X5 BTQ 101X10 250U x 1 Blend ag 25U g 2 5U 100lx1 10x Buffer 1000 lx1 BTQ 101 x5 BTQ 101 x 10 2mM dNTPs 1000 lx1 PCR
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