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1. TQ Prep Multi Q Prep CD4 T4 FITC T4 FITC T4 FITC HHR CD4 T CD4 gt CD4 NK CD8 NK CD8 CD8 T CD82. Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3 T T CD4 T CD8 2 T T CD4 CD8 T
3. T4 T8 CD4 T4 FITC T8 RD1 1096 Q T4 FITC T8 RD1 3 4 2 RE 2 3 CV T4 FITC 5 TB8 RD1 T4 FITC T4 CD4 y 1 01x 40 1 r 0 992 T8 CD8 y 0 99x 0 8 r 0 992 n 54
4. TA T8 Flow Count mt oce DxH800 mm mm x x 10 1 em 2 TQ PREP FS TQ PREP 0 5mL 3 FITC Log RD1 PE 3E Log 2
5. 10 L MSlgG1 RD1 MSlgG1 FITC 10L 4 45 5 PBS 2 3mL JA CHEHEL 400 450 xg 5 6 2 7 3 2 4 7 PBS 25 ERRE 1mL 30 2 8 260 LL IDA BELET 9 PBS 2mL 10 400 450 xg 5 10 12 9 11 13 PBS 500 u L 14 EPICS XL FC500 Navios
6. RD1 PE FITC Quadrant 1 T8 T4 Quadrant 2 T4 amp T8 Quadrant 3 T4 amp T8 Quadrant 4 T4 T8 A 1 T 1008 1888 T8 T4 CYTO TROL 6604248 Fc Fc 2
7. TQ Prep T4 CD4 DIGeorge SCID AIDS AIDS HIV HIV CD4 CD4 CD4 T8 CD8 T8 DiGeorge SCID B Epstein Barr CD8
8. CD4 T4 T8 SLE CD8 TA T8 SLE CD4 CD8 SLE CD4 T4 T8 SLE SLE CD4 CD8 SLE T4 T8 TA T8 T4 T8 CD4 CD8
9. TA T8 CD4 CD8 CD4 CD8 T4 T8 T T T4 T8 T4 T8 CD4 ARC AIDS related complex RU AIDS AIDS CD4 T4 T8 HA 0 CD8 T4 T8 CD4 CD8 MS SLE T4 T8 CD4 CD8 MS SLE
10. EDTA 20 25 6 909
11. Quadrant 1 2 4 29 19 65 n 59 T4 T8 EPICS S PLUS IV Min Max Mean 1SD T4 33 68 46 8 7 5 T8 17 47 28 8 6 8 TA mm 428 2 062 1 001 4 315 6 T8 nmm 259 1 089 627 0 189 7 T T
12. FS 90 SS FITC RD1 CYTO COMP CYTO COMP CELL T8 FITC T8 RD1 1 E T EE
13. o 1 12mm x 75mm 100L 3 10L MSlgG1 RD1 MSlgG1 FITC 10L 4 10 5 TQ Prep 6 EPICS XL FC500 Navios 2 2 2 8 C 24 2 1 2 100L
14. 1 10L 1 TQ Prep Immuno Prep TQ Prep 7546999 300 3 A Q B CHEKI 2 3 o c 2 1 6603152 300 1mL PBS 24mL 9 25 2 PBS PBS 6603369 1 500mL pH 7 2 0 2 3
15. 6604614 Wa COULTER T T8 RD1 T4 FITC 1 2 3 2012 12 1 20900AMY00164000 po E T T4 FITC T T8 RD1 FITC RD1 PE T4 FITC CD4 62kD CD4 8096 T 60 C
16. BUR MslgG1 RD1 MslgG1 FITC 6603796 50 0 5mL EDTA 3 10 x 103 mm 10x10 Emm 3 x 10 mm TQ PREP PBS 20 25 C 6 a gt 10 x 10 mm 10 20 x10 2 20 30 x10 3 30 40 x 10 5 40 60 x10 6 60 100 x10 104 100 200 x10 20 b lt 3x 107mm CD
17. T4 T8 T4 T8 FITC 468 509nm 504 541nm RD1 486 580nm 568 590nm 1 0 5mL BSA 0 2 0 01M NaCl 0 15M NaN3 0 196 T T 1 4 2 T CD4 T CD8 488nm FITC RD1 PMT 4
18. 1 0 19 2 AM RTA 2 8C T8 RD1 T4 FITC 6604614 50 0 5mL T8 RD1 5 ood o
19. 20 25 C 500 xg 5 2 3 4 DxH800 5 10 x 10 mm 1 100 L 1 Immuno Prep TQ Prep Coulter Immunology 3 A Q B C TQ Prep
20. NK T8 RD1 2 CD3 T T4 FITC T8 RD1 T CD1a T 20 25 C
21. 18 1 McMichael AJ ed Leukocyte Typing Ill White Cell Differentiation Antigens 1987 Oxford Oxford University Press p 202 206 2 Morimoto C Letvin NL Distaso JA Aldrich WR and Schlossman SF 1985 The isolation and characterization of the human suppressor inducer T cell subset J Immunol 134 1508 1515 3 Morimoto C Letvin NL Distaso JA Brown HM and Schlossman SF 1986 The cellular basis for the induction of antigen specific T8 suppressor cells Eur J Immunol 16 198 204 4 Reinherz EL Meuer SC and Schlossman SF 1983 The delineation of antigen receptors on human T lymphocytes Immunol Today 4 5 8 5 Reinherz EL Morimoto C Fitzgerald KA Hussey RE Daley JF and Schlossman SF 1982 Heterogeneity of human T4 inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations J Immunol 128 463 468 6 Meuer SC Schlossman SF and Reinherz EL 1982 Clonal analysis of human cytotoxic T lymphocytes T4 and T8 eftector T cells recognize products of difterent major histocompatibility regions Proc Natl Acad Sci USA 79 4395 4399 7 Reinherz EL Hussey RE Fitzgerald K Snow P Terhorst C and Schiossman SF 1981 Antibody directed at a surface structure inhibits cytolytic but not suppressor function of human T lymphocytes Nature 294 168 170 8 Reinherz EL Cooper MD and Schlossman SF 1981 Abnormalities of T cell maturation and regulation
22. 4 T8 T T SN No Wash No Wash TQ Prep
23. D4 CD4 T T T B T CD4 class ll MHC T8 RD1 CD8 76kD 32 34kD CD8 80 LRO T 30 3596 CD8 CD8 Em NES class I MHC T4 SFCI12T4D11 T4 ERR T BALB cJ x r2 AO REB IC NS 1 AG4 T8 SFCI21Thy2D3 T8 BALB cJ NS 1 AG4 Ig lgG1 H z L T4 T8
24. R Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantation for B cell non Hodgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 24 Reinherz EL Kung PC Goldstein G and Schlossman SF 1979 A monoclonal antibody with selective reactivity vvith functionally mature human thymocytes and all peripheral human T cells J Immunol 123 1312 1317 25 Reinherz EL Haynes BF Nadler LM and Bernstein ID eds Leukocyle Typing Il Human T Lymphocytes 1986 New York Springer Verlag p 8 9 26 Koepke JA and Landay AL Precision and accuracy of absolute lymphocyte counts Clin immunol Immunopathol 1989 52 19 27 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 7135 0063 5 7 TOC 4 4 6604614 1212
25. in human beings with immunodeficiency disorders J Clin Invest 68 699 705 9 Schmidt RE 1989 Monocional antibodies for diagnosis of immunodeficiencies Blut 59 200 206 10 de Martini RM and Parker JW 1989 Immunologic alterations in Human immunodeficiency virus infection A review J Clin Lab Anal 3 56 70 11 Fauci AS 1988 The human immunodeficiency virus infectivity and mechanism of pathogenesis Science 239 617 622 12 Collier AC Meyers JD Corey L Murphy VL Roberts PL and Handsfield HH 1987 Cytomegalovirus infection in homosexual men Relationship to sexual practices antibody to human immunodeficiency virus and cell mediated immunity Am J Med 82 593 601 13 Taylor MGJ Fahey JL Detels R and Giorgi JV 1989 CD4 percentage CD4 number and CD4 CD8 ratio in HIV infection How to choose and how to use J AIDS 2 114 124 14 Morimoto C Hafler DA Weiner HL Letvin NL Hagan M Daiey J and Schlossman SF 1987 Selective loss of the suppressor inducer T cell subset in progressive multiple sclerosis Analysis with anti 2H4 monoclonal antibody N Engl J Med 316 67 72 15 Reinherz EL Weiner HL Hauser SL Cohen JA Distaso JA and Schlossman SF 1980 Loss of suppressor T cells in active multiple sclerosis Analysis with monoclonal antibodies N Engl J Med 303 125 129 16 Weiner JL Hafler DA Fallis RJ Johnson D Ault KA and Hauser SL 1984 Altered blood T cell subsets in patients with multi
26. ple sclerosis J Neuroimmunol 6 115 121 17 Smolen JS Chused TM Leiserson WM Reeves JP Alling DW and Steinberg AD 1982 Heterogeneity of immunoregulatory T cell subsets in systemic lupus erythematosus Am J Med 727 783 790 18 Smolen JS Morimoto C Steinberg AD Wolf A Schlossman SF Steinberg RT Penne E Reinherz EL Rerchlin M and Chused TM 1985 Systemic lupus erythematosus delineation of subpopulations by clinical serologic and T cell marker analysis Am J Med Sci 289 139 147 19 Morimoto C Reinherz EL Schlossman SF Shur PH Mills JA and Steinberg SD 1980 Alterations in immunoregulatory T cell subsets in active systemic lupus erythematosus J Clin Invest 66 1171 1174 20 Raziuddin S Nur MA and Alwabel AA 1989 Selective loss of the CD4 inducers of suppressor T cell subsets 2H4 in active systemic lupus eryihematosus J Rheumatol 16 1315 1319 21 Sato K Miyasaka N Yamaoka K Okuda M Yata J and Nishioka K 1987 Quantitative detect of CD4 2H4 cells in systemic lupus erythematosus and Sjogren s syndrome Arthritis Rheum 30 1407 1411 22 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer cells T4 4B4 and increase in T suppressor effector calls T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 23 Pedrazzini AS Freedman AS Andersen J Heflin L Anderson K Takvorian
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