B4-FITC
Contents
1. 7 Fc 10 15 y 1mg mL 8 9 102. gt PBS PBS 2 8 C NBS 2 BSA 0 1961 0 19 B1 FITC gt B4 FITC 1 500L 100 000 xg 10 gt 1 5L B4 FITC 54L 195L PBS
3. B B CD4 T CD8 T CD4 CD8 AIDS HIV B B B4 CD19 B4 B Pokeweed mitogen PWM
4. 2 Ficoll Paque 1 12mm x 75mm 2 Ficoll Paque 1 x 10 1 x 10 mm 1mL 3 2 8 C 400 lt 450 x g 4 4 200wL MSlgG1 FITC 200 L 5 2 8 C 30 6 PBS1mL 2 8 C 400 450 x g 4 7 5 2 8 0 5 1mL PBS 9 EPICS Cytomics
5. 3 Ficoll Paque 1 1 x10 200L Ficoll Paque 96 200L 2 2 lt 8 C 400 450 x g 5 3 4 5 200 L MSlgG1 FITC 200L 6 2 8 C 30 7 2 8 C 400 450 xg 5
6. 2 90 3 859 4 Ficol Paque Ficoll Paque 5 5 Ficoll Paque 6
7. 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 0 196 NN 1 2 1 6603152 300 PBS 24mL 1mL 9 259 6 2 1L 8 26g 1 0g EDTA4 Na 2Na 37mg PH 7 2 lt 7 4 1 1 2 30 2
8. 11 B4 FITC 12 13 B B IgG 14 15 16
9. CYTO TROL 6604248 MSlgG1 FITC 1 296 296 T T
10. 1 15z9 B 9096 596 CD19 B T B4 89B CD19 B BALB cJ NS 1 AG4 lg lgG1 zL B4 FITC FITC Fluorescein lsothyocyanate FITC 5 lt 10 468 509nm 504 541nm 1 0 5mL 0 296 0 01M 0 15M 0 196 B B4 CD19
11. 20 25 6 a gt 10 x10 mm 10 20 x103 2 20 30 x103 3 2 4 30 40 x103 5 40 60 x103 6 60 100 x103 10 100 200 x10 20 b 3 x 10 mm 1 25 C 500 x g 5 2 3 4 LH700 5 10x10 mm 1 100L 2 Ficoll Paque 1 4mL PBS 2
12. 4 5 6 7 B4 FITC B4 FITC 2 8 24 1 28 C 2 B4 FITC 2 8 C 6 0 20 C 6 70C 12
13. 10 15 20 30 CO MslgG1 FITC B4 FITC 6602928 100 0 5mL Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL a Acridine orange Baker A366 3 0 005mg mL Ficol Paque Pharmacia 17 0840 03 EDTA 1 1 100L 3 10 x 103 mm 10x10 mm 3x10 mm PBS
14. 6603012 2009 11 2007 2 16300EZY00802000 877429 B B4 FITC 4 IO ww B B4 B4 CD19 95kD CD19 pan B B B
15. 8 3 4 9 PBS 200L 2 8 C 400 lt 450 xg 5 10 3 4 3 4 11 PBS 200L EPICS Cytomics 1 2 Ficol Paque 3 FITC Log 1 CD19 al Bs AITC
16. TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC 6603012_0911
17. B B4 CD19 1 4 488nm FITC FS 907 SS 1 EDTA 6
18. Ficoll Paque 4mL 1 3 2 8 C 400 450 x g 30 4 Ficoll Paque 5 2 8 C PBS 2 8 C 400 lt 450 xg 8 6 PBS 7 2 lt 8 C 400 lt 450 x g 4 8 PBS 9 2 lt 8 C 400 lt 450 x g 3 10 PBS 1 x10 mm 1 x 10 mL 11 a b 90 a 12 1 x10
19. Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3 Pan T Pan B T B pan B CD19 B4 B B
20. 3 20 25 C B4 FITC 6603012 100 0 5mL 1 McMichael AJ ed 1987 Leukoyte Typing Ill Oxford Oxford Universily Press p 302 306 308 315 475 2 Reinherz EL and Schlossman SF 1980 The differentiation and function of human T lymphccytes Cell 821 827 3 Reinherz EL Haynes BF Nadler LM and Bernstein ID 1986 Leukoyte Typing Il New York Springer Verlag ol 2 p 8 15 25 37 4 Drexler HG Gignac SM and Minowada J 1988 immunophenotyping of acute leukemias Bult57 327 339 5 Nadler LM Anderson KC Marti G Bates M Park E Daley JF and Schlossman SF 1983 B4 a human B lymphocyte associated antigen expressed on normal mitogen activated and malignant B lymphocytes J Immunol 131 244 250 6 Benjamini E and Leskowitz S 1991 Disorders of the Immune Response In Immunology A Short Course Seond Edition New York Wiley Liss p 211 244 Routine 4 4 7 Reinherz EL 0 Brien C Rosenthal P and Schlossman SF 1980 The cellular basis for viral induced immunodeficiency Analysis by monoclonal antibodies J Immunol 125 1269 1274 8 Felsenstein D Carney WP lacoviello VR and Hirsch MS 1985 Phenotypic properties of atypical lymphocyies i
21. 100 859 6 gt PBS PBS PBS 2 lt 8 NBS 2 BSA 0 19 0 19 PBS PBS 0 196 2 lt 8 gt 1 1 2 100L 3 200L
22. B4 3 9 CV 7 1 0 196 2 3
23. 1 x10 mm 1mL 13 2 8 C PBS 2 8 C 400 450 x g 4 14 0 05mg mL Propidium lodide 3 1 15 2 8C PBS 2 8C 400450 xg 4 16 15 17 859 b 12 25 000 1x 10 L 25 L 13 Propidium loldide 0 01mg mL 10 kL 14 30 Acridine orange 0 005mg mL 10wL 3 15 16
24. MSlgG1 FITC 200L 4 45 5 a b a 2mL 5 15 b PBS 2 3mL 400 450 xg 5 PBS 25 1mL 30 2 250 kL 6 PBS 2mL 7 400 450 xg 5 8 6 7 9 0 5 1mL PBS 10 EPICSCytomics
25. n cytomegalovirus induced mononucleosis J Intect Dis 152 198 203 9 Rinaldo CR Ho M Hamoudi WH Gui X and DeBiasio RL 1983 Lymphocyte subsets and natural killer cell responses during ytomegalovirus mononucleosis Infect Immun 40 472 477 10 Schmidt RE 1989 Monoclonal antibodies for diagnosis of immunodeficiencies Blut 59 200 206 11 de Martini RM and Parker JW 1989 Immunologic alterations in human immunodetciency virus infection A review J Clin Lab Anal 3 56 70 12 Fauci AS 1988 The human deficiency virus Infectvity and mechanism of pathogenesis Science 239 617 622 13 Pedrazzini A Freedman AS Andersen J Heflin L Andersen K Tavorian T Canellos GP Whitman J Coral F Ritz J and Nadler LM 1989 Anti B cell monoclonal antibody purged autologous bone marrow transplantatton for B cell non Hodgkin s lymphoma Phenotypic reconstitution and B cell function Blood 74 2203 2211 14 Ramos EL Turka LA Leggat JE Wood IG Milford EL and Carpenter CB 1989 Decrease in phenotypically defined T helper inducer cells T4 4B4 and increase in T suppressor effeotor cells T8 2H4 in stable renal allograft recipients Transplantation 47 465 471 15 Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 52 19 27 135 0063 5 7 TOC
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