『 Rabbit Apo B-48 ELISA「シバヤギ ®」 』取扱説明書
Contents
1. 1 2 0 1 12. 200 1 1 800 lt 2 000 rpm 10 3 ng m1 2001 200 7 1 1 250 1 250 ng ml 200 1 200 1 625 625 ng ml 200 1 200 7 1 313 313 ng ml 2001 200 7 1 156 156 ng ml 200 1 200z1 78 78 ng ml 200z1 200z1 39 39 ng ml 200 1 200 1 19 5 0 2001 0 D Apo B 48 100 1
3. 1 2 3 4 5 6 7 8 9 10 11 12 H Rabbit Apo B 48 ELISA AKRB48 Rabbit Apo B 48 ELISA KIT AKRB48 Shibayagi Co Ltd Gunma Japan 377 0007 1062 1 TEL 0279 25 0279 FAX 0279 23 0313 lt E mail gt syc info shibayagi co p lt URL gt http www shibayagn co Jp
4. ELISA 20 25 0 4m sec 80 9 0 4m sec Web 6 A 96 96 wells 8x12 1 B 1 000 ng 1 C 60 ml 1 D Apo B 48 100 1 1 E
5. Web Q amp A 20 25 C 2 10 4001 ng ml 1 250 19 5 0 1 200 200 u 1D 200 200 200 200 200 200 200 200 96 4 Apo B 48 50 1 20 25 C 1 Apo B 48
6. 0 4m sec 30 Web 10 Apo B 48 EDTA 2Na EDTA 2K 85
7. 4 lt 13 50 1 14 20 25 C 20 15 50 1 16 450nm 620nm 600 650nm 7 Strip 1 amp 2 Strip 3 amp 4 Strip 5 amp 6 Strip 7 amp 8 Strip 9 amp 10 Strip 11 amp 12 A 1 250ng ml 1 9 17 25 33 B 625ng ml 2 10 18 26 34 C 313ng ml 3 11 19 27 35 D 156ng ml 4 12 20 28 36 E 78ng ml 5 13
8. 2 PPPE 10 5 10 1 800 2 000rpm 10 3 160mg dL
9. 100 4 Apo B 48 50 1 20 25 C 1 SV ZZ 40 tale Sa aii 4 NN 50 1 20 25 80 4 TMB TMB 501 20 25 C 20 1M HzSO 501 we 3 gt ES 450nm 620nm 600 650nm OE
10. 6 3 plasma Hipoprotein B48 ApoB 48 264kDa VLDL LDL HDL B100 48 Chylomic
11. 5 20 25 C 1 6 4 lt 4 7 Apo B 48 50 1 8 20 25 C 1 9 4 lt 10 50 1 11 20 25C 30 12
12. 20 25 C 2 6 B ApoB 48 1 000 ng 400 1 10 1 800 2 000 rpm 10 3 24 85C
13. C 100 E 100 1 C 100 Q 10 x 10 x 10 100ml 10 x 900ml 96 A 96 2 8 C B C CTMB
14. 10 4 300 1 8 19 5 ng m1 OD OD 1 4 5 8 ei 5 25ml 7 600 1 200rpm 10 3
15. 160mg dL 160mg dL Apo B 48 100 500KIU KIU kallikrein inhibitor unit 35 11 1 4 lt 2 501 3 50 1 4
16. 4C8 4C8 Apo B 48 1 Apo B 48 Apo B 48 1 TMB 450nm 620nm Apo B 48 5 ELISA
17. Q 1 A 1 Q 2 A 2 Q amp A 16 1 Determination of apolipoprotein B 48 in serum by a sandwich ELISA Kinoshita M Kojima M Matsushima T and Teramoto T Clinica Chimica Acta 351 115 120 2005 2 Effects of intensive atorvastatin and rosuvastatin treatment on apolpoprorein B 48 and remnant lipoprotein cholesterol levels Otokozawa S AiM Van Himbereen T Asztalos BF Tanaka A Stein EA Jones PH Schaefer EJ Atherosclerosis 205 197 201 2009 3 Determination of Immuno reactive Rabbit Apolipoprotein B 48 in Serum by a sandwich ELISA Kinoshita M Kojima M Matsushima T Mashimo Y Kigure B and Teramoto T Exp Anim 59 4 July 2010
18. 100 1 1 F TMB 12 ml 1 H 1M HzSO4 12 m1 1 1 10 x 100 ml 1 4 1 7 50 1 200 1 Eppendorf multipette plus 50 1 Vortex 600 1 200 rpm 96 Web 96 450 10 nm 620nm 600 650nm 8
19. 18 4 15 Q amp A 1 2 3 4 5 6 7 19 5 ng ml OD OD 4 5 8 CV 1 2 3
20. 2 8 C D Apo B 48 RE 2 8 C HD IM HzSO 2 8 C 10 x 10 x 2 8 C 9 1 1 96
21. 21 29 37 F 39ng ml 6 14 22 30 38 G 19 5ng ml 7 15 23 31 39 H 0 8 16 24 32 40 12 1 X ng ml Y Web ELISA ng ml C 3 20 lt 25
22. 2015 4 20 Rabbit Apo B 48 ELISA Web Q amp Al 13 x 10cm 1 B 48 Apo B 48 2 50 Apo B 48 10 1 1 96 2 2 8 C
23. 3 4 SUNRISE RAINBOW TECAN 18 Apo B 48 19 5 1 250 ng ml ELISA Apo B 48 B 100 5 3 C V 5 2 2 4 C V 5 2 3 Apo B 48 93 4 105 2 3 R2 0 9987 0 9996 14 Apo B 48 1 12 gml SE 0 13 g ml N 6
24. ron CM ApoB 48 CM ApoB 48 LDL HDL B48 2 CM CM CETP Cholesterol ester transport protein 4 Apo B 48 4C8 ApoB 48 Apo B 48
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