The MFCS Manual
Contents
1. 23 5 93 N92 Vi EE 23 5 9 2 Labview examples nennen nennen nnn nnns nai nn nnns na arn n rns 24 2o ERE hc 26 5 4 1 Function description preliminary ss 26 5 4 2 VBA declarations examples sn 28 5 5 ABOUT OUTPUT PRESSURIZATION e 29 6 APPENDIGES nc ana nee en na ee oc as cs 30 6 1 TECHNICAL SPECIFICATIONS MEGA 30 6 2 ACCESSORIES REFERENCES AND SUPPLIERS eene meer ern 31 6 3 RESPONSE AND STABILIZATION TIME 32 6 4 UNIT CONVERSION TABLE 32 6 5 HYDRODYNAMIC RESISTANCE OF RECTANGULAR CHANNELS AND NETWORKS 33 6 6 MAINTENANCE e 35 6 7 TRACE AND LOG FILES FORMAT 36 VS BH N N R FL UIGENT MFCS User Manual 1 Introduction To your application Fluiwell J9 Reservoirs The MicroFluidic Control System MFCS is a High precision pneumatic pressure controller designed to handle fluid in microfluidics systems microchannels and nanochannels capillaries Lab on chips It allows a stable and pulsation free flow with short response time 100 ms and a stabilization time as low as 1s With the MFCS it is also possible to control several independent channels up to 16 cf Labiview VI at the same time The user friendly softwares allow you to create scripts for complex flow patterns or dynamic coupling for user controlled dependence between channels Pressure Range 0 to 25 0 to 69 0 to 345 0 to 1000 25 to 0 69 to 0 mBar mBar mBar mBar2. SUGGESTIONS DYSFUNCTIONS Name Urgent yes Suggestion Society Dysfunctions L Device Version Software Version Menu or concerned function Error code if available DESCRIPTION please cite error messages operation before error operation to quit error message add screen shots if possible Don t write in this field please 38 LK FLUIGENT MFCS User Manual FLUIGENT P pini re H pital COCHIN 29 rue du Faubourg St Jacques 75014 Paris France Phone 331 46 33 16 28 Fax 331 46 33 16 68 website http www fluigent com e mail supportmfcs fluigent com 39
3. 1L Bile Setup Help AN d FLUIGENT Valve output Valve or Pressure control MFCS Statue Manual auto Active config 2 Food back coetniciont 504 BEE Bie Setup Help Chan 1 Chan 2 Chan 3 Chan 4 ED E EU oo Be E FLUIGENT decreasing with the same variation Both parallel and antiparallel coupling are possible To set a parallel coupling between channel 1 and channel 2 choose for both channel in To achieve an antiparallel coupling just choose A for one of the two channels To couple more than one channel set the coupling field of each channels in consequence Chan 1 Chan 2 Chan 3 Chan 4 Valve output ai EX us as a0 Valve or Pressure control REH X 25 744 3 073 130 a b 50 k v Auto fv Auo ke A 2 lig 3 Conf lt oha 1 Ear Con 47 Ld C Doc uments and Setthigs cfiBureaviLintitied 0P TRACE OFF LOG OFF K Documents and Serasi sared m cs Coupling ae zl T us E a Maximum pressure 2 ses E Lea F peraling Run Requested pressure 10223 13073 582 0780 Iv perating Run ee mers Measured Ur e bad Ki L RENTE Exceeding input pressure L BD w Aula Requested pressure 1 2 3552 Measured pre Active config Config a os rms Re aa Foodbackcoemeiom Sal vaf ail Sa Manual auto v Auto fv Auo jv Aus v Aula Moto A Config 2 Config Zong 4 7 TRACE OFF Los Et Figure 8 Anti parallel coup
4. ab Q C 7 Ap abu b U 9 L a with channel dimensions a and b with a b gt gt 1 channel length and viscosity u 9 5 107 Pa s for H2O at 25 The relation between the friction C and the aspect ratio a b can be calculated Stone Stroock Ajdari Ann Rev Fluid Mech 2004 uc P gw 1 T es 2 1 au tanh BE p In with 2n 1 a D H 7 TU c Sp b L 12 0 08 0 06 0 04 0 02 0 1 10 100 Figure 6 This graph shows the friction coefficient as a function of the aspect ratio of the channel and can be used to estimate the flowrate due to a given pressure difference Typical values are 0 1 gt C 0 03512 1 1C20 05708 1210 Cz0 07688 Example A channel of 10x100um with a length of 10mm contains 0 01 ul Applying a pressure difference of 10 Pa 2100 ubar gives a volume flow rate of about Q 0 0005 ul min and an averaged flow velocity of u 5 um min A pressure of 100 000 Pa 21 bar is 10 000 times higher thus the flow rate and velocity are also 10 000 times increased Since Ohm s law also applies to flow of other particles than electrons e g water we can transfer the formulas For one single channel we have for the volume flow Q as a function of the externally applied pressure difference p and the resistance R Q p R Q abu The pressure pc at an arbitrary position in a single channel with partial resistances R1 and R2 of t
5. exe mMFCS Control 1 08 The software is ready to ES be used Chan 1 Chan 2 Chan Be Valeoupae 50 ro oo FLUIGENT wen 2 3 yt Pressure control Gd e WARNING PREHEATING PROCEDURE Peace switch onthe GREEN bution on the MECS to start procedure and want for 10 minutes Remsring ime 19 00 DS Coupling D D D Se Maximum pressure mbar 2 000 DU Operating Direct control Requested pressure mbar 200 mm Exceeding TGP pesen Purge pid dede Measured pressure mbar 3 000 0 090 Pur ge Kctpscamente and Settings r len TANIGA Suraat MFCS 4C nded mfzs MFCS 8C for a 8 channel device FLUIGENT MFCS User Manual For positive pressure models The pressure source should be CLEAN AND DRY and fixed to the input pressure value specified on the rear panel of your MFCS Pressure Range mBar Input Pressure value mBar Please find besides the value of the input oressure MFCS Preheating procedure Press the Green button and set the pressure source to the input pressure value of your MFCS You can monitor the pressure source value on the front display The preheating will start Unpack the Fluiwell optional accessory Screw the 2 mL reservoirs on the fluiwell 800 1300 500 500 A preheating procedure is necessary to warm up the MFCS 10min meanwhile you can continue with the next step Avoiding this step can decrease the pressure control stabi
6. from the MFCS outlets Use the provided spanner or any 4 tools Protection cap Free outlet Tightly screw the soft tube adapters on the outlets Soft tube adapters Connect the soft tubes to the MFCS outlets Connect the fluiwell to your microfluidic chip using the FEP tubes for example A list of adapters for smaller inner diameters tubes is available in the appendices 13 FLUIGENT MFCS User Manual Fill up the 2 mL reservoirs with the appropriate fluid corresponding to the inlets of your chip At the end of the preheating NS In order to guarantee countdown Setup Help the accuracy of your push the red button and measures we calibrate the MFCS by clicking Compensate off set recommend you to on the Setup Compensate I calibrate the device offset T Purge warning every time you move it Otherwise once a Offset compensation month is sufficient The MFCS is ready to oe be used value Click save to MFCS to load your calibration parameters into the MFCS and close the Chan 1 417 window Chan2 420 Chan 3 416 Chan 4 417 14 FLUIGENT MFCS User Manual 4 Basic Software Operations Here you will learn how to operate the MFCS using the MFCS control interface 1915 Fie Setup Help Preheating Chan 1 Chan 2 Chan 3 Chan 4 H Valve output 0 0 0 0 0 0 a Warning K Ri NEU E Valve or 5 Pressure control H R H ARNI
7. 32 i float ByVal Single pointer on float SGL by address ByRef Single NB 1 in Visual Basic all values are signed 2 After calling mfcs_initialisation a delay 0 5s must be set before calling other functions 3 A mfcs_close must be called before leaving the application to avoid Windows error 5 4 2 VBA declarations examples Public Declare Function mics initialisation Lib ds mics mics _vb dlli ByVal i As Integer As Long Public Declare Function mics close Lib d mfcs mics vb dl1i ByVal H As Long As Byte Public Declare Function mfcs read Lib d mfcs mfcs_vb dll ByVal H As Long ByVal S As String As Byte Public Declare Function mics write Lib d mfcs mfcs vb dll ByVal H As Long ByVal S As String As Byte Public Declare Function mrcs set purge on Lib ds XmfosWimfos vb dll ByVal H As Long As Byte Public Declare Function mics set purge off Lib d WnfosWimfcos vb dll ByVal H As Long As Byte Public Declare Function mics get purge Lib drXmfosXmfos vb dll ByVal H As Long ByRef C As Byte As Byte Public Declare Function mfcs get status Lib d mfcs mfcs vb dll 28 FLUIGENT MFCS User Manual ByVal H As Long ByRef C As Byte As Byte Public Declare Function mfcs read chan Lib d mfcs mfcs_vb dll ByVal H As Long ByVal Chan As Byte ByRef P As Single ByRef Chrono As Integer As Byte Public Declare Function mics data chan Lib ds mics mics vb dlr ByVal H As Long ByVal Chan As Byte ByRef
8. Depends on model 30 INE FLUIGENT MFCS User Manual 6 2 Accessories references and suppliers Designation Reference and Supplier Remark USB Cable CD Software User manual Blue tubing Upchurch Scientific 1581 OD 1 32 ID 250um FEP tubing Glass capillary Polymicro technologies TPS100375 OD 360um ID 100um Green Sleeves Upchurch Scientific F 242X 1 16 to 360um Green Sleeves Upchurch Scientific F 247X 1 16 1 327 10 32 Peek Fitting Nuts Upchurch Scientific F 120 soft tubing 1x3mm Fisher A31309 31 VS B R NR FL UIGENT MFCS User Manual 6 3 Response and Stabilization Time Here are the graphic definition of response time and stabilization time Pressure zm em em em em em mm em em em d em em em em em CC oo e e e s C O NEE ES 595 ilizationTime Resp nse Time 6 4 Unit Conversion Table value 1 factor kPa bar psi xunit 1 kPa 1 0 01 0 145 1 bar 100 1 14 5 1 psi 6 895 68 95x10 1 1 inch H2O 0 249 249x10 3 612x10 Example 10 kPa 10 kPa x 0 01 bar kPa 0 1 bar 100 Ti inch H 0 4 016 401 6 2 68 1 32 FLUIGENT MFCS User Manual 6 5 Hydrodynamic Resistance of Rectangular Channels and Networks Pressure difference Lip the volume flow rate Q or the averaged velocity u and the friction coefficient C are related through 3
9. EN 1 The manual control of the Error in electrovalve is not recommended and the output pressure is no longer regulated Disable purge feature channel 1 can be used normally mfcs set purge off vi Handle in Handle out Error in Error out mfcs set purge on vi Enable purge feature channel 1 is Handle in directly connected to the pressure Kos mangle Our supply Please use with care in order to Error in Error out avoid any damage to your microsystem Calibrate the zero pressure value of the Handl Si iow Handle out specified channel This value can be Channel igi Geer Error out estimated with mfcs data chan vi Error in mfcs set zero vi 5 3 2 Labview examples MFCS 8C control Serial Number Requested MFCS Serial Number reference This VI is a transposition of MFCS control It px y ll integrates MFCS control software s functions in E EE a Labview environment 5 80 0 under process qt Se Enter serial number 40 0 1 SECH O MFCS e reset MFCS needs to be manually rearmed green switch on the front panel C al i b rat e t h e M F C S if n e e d e d D 1 1 1 1 1 1 1 Normal 3768 3780 3790 3800 3810 3820 3827 ressure Supply Overpressure Gees SE EE S et H p ressure O rd e e an d N al D h a Channel 1 Channel 2 Channel 4 UN a G aa dene d feeback coefficient in the channels of Go M EE Interests Ze d d um Press purge channel 1 only to fill or Ic Au Gus Gu empty y
10. NG PREHEATING PROCEDURE Direct Please switch on the GREEN button on the MFCS to start procedure and wait for 10 minutes Button Remaining time 10 00 MFCS tf E ST Status US oen owe I A1 SI 97 2 Standby Maximum pressure mbar oo 000 oo 009 P urge id input pressure suisse Requested pressure mbar 0000 oo oo wo H Purge Dn Measured pressure mbar oam 000 om 0000 Button o Feed back coefficient S 52 Sai Purge Manual auto v Auto v Auto k IV to Active config Ve Config 20e sisi Documents and Settings v lan TANIGA BureauiMFCS 4C Unttled mfcs TRACE O 1sOFF LOG OFF Pressure Handle Pressure scroll Coupling field Requested Pressure field Config Tabs For negative pressure models the software displays the absolute value of the pressure 4 1 Purge Click on the Purge button and valid the warning Warning Do vau really want to purge on channel 1 Do not ask this question again Disable the purge feature by clicking again on Purge button SG USE open Standby Operating Exceeding input pressure Purge Un Purge A purge feature is available in channel 1 It applies maximum pressure on this channel It suits any filling or emptying purpose The Purge on green indicator will appear until you stop the purge 15 FLUIGENT MFCS User Manual 4 2 Pressure control in the channels For negative pressure mode
11. O A computer USB 1 1 port or faster screen resolution of 1024x768 Intel Pentium II 500 MHz or faster Windows2000 Windows XP 5 Mo of Free Hard disk space O O O O O A pneumatic pressure source o For positive pressure models dry and non corrosive use an air drier dust and oil free with a pressure regulator to tune the input pressure value 0 01 um filtered The pressure range of the pump should suit your MFCS input pressure see Figure 3 o For negative pressure models Usea pump in aspiration mode LJ U D DU U Norgren Norgren F07 200 A3TG F72C 2GD STO Particle filter 0 01um Gas Cylinder Pump of Local Pressure Network Pressure Regulator Watson Smith 100300R Only for positive pressure Figure 5 Suggested Pressure Source scheme FLUIGENT MFCS User Manual aa 0 to 25 0 to 69 0to345 Oto 1000 25 to 0 69 to 0 ange mBar mBar mBar mBar mBar mBar DES 500 mBar 500mBar 800mBar 1300 mBar 500 mBar 500 mBar Figure 6 Input pressure value according to MFCS pressure range Connection to the MFCS requires either The Fluiwell a microfluidic interface between the MFCS and your Microdevice that uses 1 16 OD tube or smaller see Figure 7 Your own connection system To microfluidic application Controlled pressurized gas from MFCS Figure 7 Fluiwell s working principle FLUIGENT MFCS User Manual 3 Installation guide 3 1
12. Su As Byte ByRef Sm As Integer ByRef Zr As Integer ByRef Ms As Integer ByRef Chrono As Integer As Byte Public Declare Function POS get serial Lib d mics mtcs vb dLl ByVal H As Long ByRef Serial As Integer As Byte Public Declare FuncEIOn mics set auto Lib ds mics mics vb dll ByVal H As Long ByVal Chan As Byte ByVal P As Single As Byte Public Declare Function mics set alpha Lib d mfcs mfcs_vb dl1 ByVal H As Long ByVal Chan As Byte ByVal Alpha As Byte As Byte Public Declare Function mics set manual Lib di mics mics vbo adl ByVal H As Long ByVal Chan As Byte ByVal Ev As Single As Byte Public Declare Function mics set zero Lib dr mics mics vb dll ByVal H As Long ByVal Chan As Byte ByVal Zero As Integer As Byte 5 5 About output pressurization Pout Pin Pout Atmosphere In order to operate in an optimized range of the pressure full scale it is recommended to work with differential pressure Indeed by pressurizing both input and output of a channel properly the pressure inside the channel would be equal to the differential of the output and the input pressures 29 KG B R N P FL UIGENT MFCS User Manual 6 APPENDICES 6 1 Technical Specifications MFCS Characteristics Input pressure range Output pressure precision Min output pressure step Max output pressure Operation temperature range Input voltage range Weight Power consumption Characteristics Input pressure ran
13. VI for automated pattern setting To save a set of curves first Sall Setup Help choose the desired trace rate Open Save Save as Save log E N T Save trace 1s 10s Then start recording by giving a XE Setup Help name to the curves file in save tees trace dialog box of the file menu a tick will appear Save Save as Save lag Save lace Trace rate Exit 17 FLUIGENT MFCS User Manual End the record by clicking on SEN Setup Help save trace untick Open Save Save as Save lag The file is a text file that can be RL use txt imported in any spreadsheet Start Title line Curves File format d 03 09 2020 08 15 2 1 39 MFCS Automat V 1 05 MFCS S N 0005 V 000B see section 6 7 sall maea raoa moor al ul For negative sel masa aa L oor ol oe pressure models 152120 Ass ua Ana L p ol A ul displays the the software absolute value of 03 09 2020 08 15 2 2 05 E x the pressure Time of Time Pressure Pressure Pressure Pressure End Title experiment Step n Channel 1 Channel 2 Channel 3 Channel 4 line 18 KG B R NR FL UIGENT MFCS User Manual 5 Advanced Control of the MFCS 9 1 5 1 1 Coupling More about MFCS Control The coupling is a feature that creates software driven dependence between channels Thus it is possible to increase pressure in one channel while the pressure in a second one is the coupling field
14. Warnings O MECS must be used in a clean and dry environment O No liquid should enter into the device otherwise this would void the warranty O The Pressure source must be dry dust and oil free Use a 0 01 um filter and a pressure regulator to insure proper input pressure for positive pressure models 3 2 Unpacking and preparation of the MFCS with Fluiwell Please follow the next series of operation in order to set properly your MFCS Indication Photos Remarks Unpack the MFCS Please check that all is in order lf any damage please phone us or e mail us mfcssupport fluigent com Connect to the rear c our To avoid liquid back flow please panel e ESI wu use protection systems such as the usb cable k the FLUIWELL see Figure 7 the power supply EE L the pressure source For positive pressure models PLEASE USE CLEAN AND DRY GAS WITH A PRESSURE REGULATOR BEFORE THE MFCS see section 2 3 FLUIGENT MFCS User Manual Insert the MFCS softwares CD Copy the MFCS 4C folder to desired Labview drivers MECS 4c Pressure fli location on your hard v1 ni drive Open the MFCS 4C folder you just copied on your hard drive MACS user mes manual pdf Y1 08 4 exe Switch ON the MFCS rear panel HVH FC The red light should be bright If not check the power supply and the USB cable Double click on the MFCS control icon MACS user MFCS manual pdf Y1 08 4
15. ally set Comment define a comment of the current action LI U D U EI 20 FLUIGENT MFCS User Manual 5 2 3 Descriptions of the Actions Actions Parameters Description This value is linked to the feedback coefficient for the pressure regulation default value 5 Integer from 0 to 255 n to the ERE r GE channel Endprogamme Goto label Go to the line with the s string the label row The channel is control manualy Manuel Do be without feedback E X is equivalent to the 96 of alimentation of the Electrovalve Purge off Stop Purge on channel 1 Pugeon Start Purge on channel 1 secondes Wait 5 2 4 Script example Here is an example of a script with the corresponding pressure chart Step Label Action Channel Parameter Unit 1 alpha 1 5 2 alpha 2 5 L S smt ato 1 175 fmb wait ee S oe 1 210 mb 6 auo 2 175 mb H qm EE eg 8 Jato 2 140 mb CE label start This is an endless loop generating a 4 seconds period square pressure signal Pressure mBar Example of MFCS Mat script for channel 1 and 2 250 200 O1 eo Channel 1 Channel 2 wesch CH CH 50 0 50 100 150 200 250 Time s 21 VK BH R RR FL UIGENT MFCS User Manual 22 FLUIGENT MFCS User Manual 5 3 Labview The Labview drivers are in the provided CD The Lab
16. annels and on the time constant of the phenomena under investigation 5 2 MFCS mat scripting software 5 2 1 General description IB MFCS Automat 1 06 nmi xl File Program Help wan berg ees Ten ees oases Ta i Wait timer R Counter value BE Program stoppped Table here it is blank K r MFCS Status USE open Standby Operating Exceeding input pressure Purge On Chan 1 1 al i a Chan 2 Chan 3 Chan 4 EI imulation made TRACE O 1sOFF LOG OFF For negative pressure models the software displays the absolute value of the pressure MFCS mat is a script software that allows to create automated pattern of pressure In the main window you can find Achart showing the pressure value of all the channels here it is blank L Below the chart the MFCS status is shown with the buttons play pause stop et verify program allowing one to play pause stop and verify the programme Onthe right hand side the user can fill in different actions in the table script 5 2 2 Description of the table In this table there are 6 rows MFCS Stat s Label needs a string It is where the action Goto label looks for Action define the type of action Ch define the channel number where the action is applied Parameter define the parameter of the action if necessary Unit specify the unit of the parameter automatic
17. d to all channels mfcs set alpha Set alpha value default value 5 for the specified channel from 1 to 4 or 8 If the specified channel is 0 the same is applied to all channels Alpha is linked to the proportional value of the PID mfcs set manual Set the electrovalve alimentation 96 for the specified channel from 1 to 4 or 8 If the specified channel is 0 the same is applied to all channels The manual control of the electrovalve is not recommended and the output pressure is no longer regulated Save in the EEPROM the zero pressure value of the specified channel 26 FLUIGENT MFCS User Manual The following array gives an algorithmic description of the previous functions mfcs initialisation UL handle US serial number Initialise USB connection and look for a MFCS with the specified serial number handle 0 if no USB connection mie gess BOK UL handle Close USB connection C error UL handle Read string on the interface C error UL handle Write string on the interface mfcs set purge on C error UL handle Open purge on channel 1 mfcs set purge off C error UL handle Close purge on channel 1 mfcs get purge C error UL handle Get the purge state PB purge state C error UL handle Get MFCS status 0 if the MFCS is reset PC Status 1 if normal 2 if overpressure 3 if MFCS needs to be rearmed mfcs read chan C error UL handle Read the pressure value mBar of the specified channel with the timing tim
18. e unit 25ms PUS chrono mfcs data chan C error Head the sensor data sensor unit 1 H2O 2 4908 mBar 2 psi 68 946 mBar full scale in pressure unit zero value sensor U12 direct pressure measure U12 chrono time unit 25ms PUS chrono C error UL handle Get the serial number of the MFCS PUS Serial mfcs set auto C error US handle Regulate pressure mBar on the specified channel if 0 the same for all channel mfcs get status mfcs get serial 27 FLUIGENT MFCS User Manual mfcs set alpha C error UL handle Set alpha value U8 This value is linked to the PID performance The recommended value is 50 for MFCS device sold with MFCS Control C channel V1 05 For more recent version V1 06 and V1 07 the default value is 5 and a preheating of the electro valves is necessary 4596 alimentation for 10 min mfcs set manual C error UL handle Set electro valve voltage 96 on the specified channel if 0 the same for all channel F EV gam CCE mfcs set zero C error UL handle Save Zero sensor value on the firmware To get this value use mfcs data chan PUS mesure Definition of C error 0 OK 1 USB closed 2 Wrong channel bits C Labview VBA __ 8 unsigned char U ByValByte U8 by address ByRef Byte char for boolean result 1 true O U8 ByVal Byte 32 U8 by address ByRef Byte flot 0
19. ei d FLUIGENT MicroFluidics Control System User Manual MECS software 2009 Fluigent MECS User Manual version 2 0 www fluigent com VK BH R RR FL UIGENT MFCS User Manual Ls FLUIGENT MFCS User Manual Table of Contents 1 INTRODUCTION neue ENNEN NN ENNEN EN ENNEN enhn enun nnn d 2 PACKAGE CONTENT AND REQUIRED MATERIALS 5 2 1 PACKAGE CONTENT enne eminere mea emis sesseseaesease sa sessa ease esses sese eue 5 2 2 MFCS DESCRIPTION 6 2 3 REQUIRED MATERIALS e 6 3 INSTALLATION GUIDE e esee n nnn 8 32l WARNINGS RR 8 3 2 UNPACKING AND PREPARATION OF THE MECS WITH FLUIWELL 8 3 3 STARTING THE d OS vas N ENEAKO O EEY 13 4 BASIC SOFTWARE OPERATIONS ee EN ENNEN ENKEN ENKEN E 15 M EMEN POUR 1 REP 15 4 2 PRESSURE CONTROL IN THE CHANNELS eese eminens ie resea areas 16 4 3 TABS CONFIGURATIONS nee ene meses emissis sesea sess sesss ease essere agen 17 4 4 SAVE CURES 17 5 ADVANCED CONTROL OF THE MFCS 19 5 1 MORE ABOUT MFCS CONTROL 19 5 1 1 bem 19 5 1 2 ER e EE 19 5 1 3 Feedback Coefficient 19 5 2 MFCS MAT SCRIPTING SOFTWARE eee 20 5 2 1 Ee e Te EE 20 5 2 2 Wi teweiekeoimin Aiie m 20 5 2 3 Descriptions or tne ACIIOFIS EE 21 5 2 4 ITE PT 21 JOE Ulys
20. for a wide range of microfluidic applications in particular those involving extremely low pressures and or volume flow rates ul to pl min However when using large volumes an adjustment of the feed back 19 Chart FLUIGENT x FLUIGENT MFCS User Manual loop s reactivity can be necessary to achieve an optimal operation of the MFCS This can be achieved independently for each channel by changing the feed back coefficient The factory setting is 5 a smaller coefficient results in a slower reaction a higher value gives a faster response If the feedback coefficient is to high the regulation loop can become unstable and pressure control inside the channel is no longer possible The optimal response is obtained just below the critical damping situation between a monotonous return to equilibrium and oscillatory overshooting Please note that for high pressure versions of the MFCS at the factory setting initial value operation is oscillation free for small pressure changes but shows overshooting and transient oscillations for drastic pressure changes If this overshooting is a nuisance to your application it can be suppressed by decreasing the feedback coefficient at the expense of the regulation speed for small pressure changes A working compromise has to be found the optimal value depends on several features of the connected system volume and elasticity of tubing hydrodynamic resistance and geometry of the microfluidic ch
21. ge Output pressure precision Min output pressure step Max output pressure Operation temperature range Input voltage range Weight Power consumption Characteristics Input pressure range Output pressure precision Min output pressure step Max output pressure Operation temperature range Input voltage range Weight Power consumption Characteristics Input pressure range Output pressure precision Min output pressure step Max output pressure Operation temperature range Input voltage range Weight Power consumption MFCS 25 mBar Min Typ Max Unit 500 600 mbar 2 5 96 full scale 23 ubar 25 mbar 10 25 50 C 23 24 25 V 1 9 2 25 kg 16 W MFCS 70 mBar Min Typ Max Unit 500 600 mbar 2 5 96 full scale 63 ubar 69 mbar 10 25 50 C 23 24 25 V 1 9 2 25 kg 16 W MECS 350 mBar Min Typ Max Unit 800 900 mbar 2 5 96 full scale 0 32 mbar 343 mbar 10 25 50 C 23 24 25 V 1 9 2 25 kg 16 W MECS 1000 mBar Min Typ Max Unit 1 3 1 4 bar 2 5 96 full scale E 0 95 mbar g 1033 L mbar 10 25 50 C 23 24 25 V 1 9 2 25 kg 16 W Comment Better if calibrated see Quick start Depends on model Comment Better if calibrated see Quick start Depends on model Comment Better if calibrated see Quick start Depends on model Comment Better if calibrated see Quick start
22. he two halves of the channel is 33 Ls FLUIGENT MFCS User Manual Dr E DF Q Q2 S RE The total resistance is R R1 R2 the flow yields Q p1 pc R1 R2 is equivalent If the two parts are of equal resistance equal length and diameter we find the well known average value pc p1 p2 2 For three channels p1 p2 p3 and R1 R2 R3 joining in one point with pressure pc we find using the continuity equation the sum over all Qs going into one knot vanishes P3 P Po Ps R R R 93 R R R R R R Q Q PI P4 Pc Hz The corresponding flow in channel 1 as an example can be obtained with Ohm s law Q1z p1 pc R1 the other values are equivalent To obtain the corresponding formulas for n channels joining in one knot the latter formula can be generalized n Pi I gt Ri More complex geometries can be calculated using well known methods in analogy to electrical resistor networks pressure is equivalent to the electric potential flow is equivalent to the current and flow resistance of a channel plays the same role as the Ohm s resistance Pe 34 VS B R NR FL UIGENT MFCS User Manual 6 6 Maintenance The MFCS is designed as a very robust device for usage in laboratory and industrial environment and easy to maintain For optimal accuracy calibration should be performed regularly typically every month or after the MFCS has been displaced in order to maintain optimal accuracy of the res
23. ling allows changes in the pressure difference of two channels without changing the pressure sum Parallel coupling keeps the pressure difference constant Shortcut It is possible to disable temporally the coupling feature of a set of channels by pressing the shift key Then you are able to move the handles independently 5 1 2 Manual auto The manual mode provides a proportional control on the valves The handles directly control the valves opening and no regulation occurs This mode is only adapted for special use and it will be subject to intrinsic nonlinear characteristics hysteresis and limited reproducibility of the solenoid valves The auto mode provides a direct control on the pressure inside the channels The handles set the requested pressure and the embedded regulation program will compute the order to give to the valves in order to have a stable quick and reproducible response 5 1 3 Feedback coefficient 400 4 300 4 250 200 4 Feed back ee coefficient too high 50 4 0 T 50 9 5 10 15 20 300 p Optimised Feed 150 back coefficient 100 50 0 T 5o 9 S T n ES 300 250 4 200 150 100 50 400 350 0 Feed back coefficient too low 50 U Figure 9 effect of the Feed back coefficient value on the pressure stability and response time The MFCS Control software provides optimal pressure and flow regulation
24. lity and accuracy When performing series of experiments the preheating is only necessary once a day For negative pressure models LCD panel displays the absolute value of the pressure Please check that the 4 toric seals are in place before mounting the reservoirs 10 SEXENJFLUGENT MFCSUserManal 1 MFCS User Manual Prepare your experimental setup by placing all the devices and elements you might need The lengths of the pieces correspond to the distance between the fluiwell and the MFCS If you use different connectors a list of compatible adapters is available in the appendices 4 pieces of the soft tube 4 pieces of the FEP tube Connect the soft tubes to the fluiwell Connect the FEP tubes to the top outlets In order to avoid leaks the fitting should be tightly screwed 8 pieces for MFCS 8C with 2 fluiwells Microfluidic chip Microscope Soft tube FEP tube 1 16 provided connectors You can find the references of the tubings in the appendices The FEP tubes should be visible inside the 2 mL reservoirs 11 FLUIGENT MFCS User Manual Now your fluiwell is ready to be connected to your microfluidic chip and to the MFCS Don t forget to fill the reservoir with your sample 12 FLUIGENT MFCS User Manual 3 3 Starting the MFCS Please proceed as follow EE the protection caps
25. ls the software displays the absolute value of the pressure There are two different way to modify the pressure in a channel Press Direct Control button to Otherwise orders will activate the control of the not be transmitted MECS Direct control Change pressure in the channels using the corresponding handle Enter the numerical value of the pressure in the requested pressure field Maximum pressure mbar 3 290 Requested pressure mbar 40 417 Measured pressure mbar 40 459 EZ Feed back coefficient ier Shortcuts It is possible to modify the positions of the handle using keyboard Click on a handle then e Up arrow Move the handle up by 1 steps Down arrow Move the handle down by 1 steps Page up Move the handle up by 10 steps Page down Move the handle down by 10 steps Move to the top of the scale using home Move to the bottom of the scale using end 16 FLUIGENT MFCS User Manual 4 3 Tabs configurations For negative pressure models the software displays the absolute value of the pressure You can use the config tabs tO Lean 4 chan 2 chan 3 Chan 4 set different handle patterns in order to easily switch between several pressure sets by clicking on the corresponding tab xp si sg 75258 7619 75279 75258 40417 21187 63960 51147 51168 ss ss SA 53 Auto W Auto W Auto N Auto The curves will follow the pressure pattern See MFCS mat or labview
26. mBar mBar MFCS 4C MFCS 4C MFCS 4C MFCS 4C MFCS NEG MFCS NEG 25 70 345 1000 4C 25 4C 70 MFCS 8C MFCS 8C MFCS 8C MFCS 8C MFCS NEG MFCS NEG channels 25 70 345 1000 8C 25 8C 70 type Figure 1 Available MFCS series VK BH R R FL UIGENT MFCS User Manual 2 Package content and required materials 2 1 Package content The MFCS package comprises following items One MFCS unit L A power supply and a power line cable O A USB cable L A pneumatic Connection kit 4 soft tube adapters 4 x 50 cm of soft tube OD 3mm ID 1mm a 4 spanner L A quick user guide This user manual User manual Power supply Soft tube Power line cable Adapters MFCS softwares CD 2 m soft tube USB cable 1 4 Spanner MFCS Figure 2 Package content Optional Accessories A Fluiwell and a set of spare parts FEP Caps Fluiwell Reservoirs Figure 3 Fluiwell package If any parts are missing or damaged please contact your local dealer or Fluigent immediately For a 8 channel MFCS 8 adapters will be provided soon provided in the MFCS package VS B R NR FLUIGENT MFCS User Manual 2 2 MFCS Description Pressure Inlet USB Power Supply Rear View On Off Switch LCD Pannel Displays input pressure when grren button on Front View Pressure outlet Green Button Red Button Figure 4 Front and Back views 2 3 Required Materials Using the MFCS requires the following materials
27. nel The vi returns the handle and a boolean that confirms that the USB initialized correctly IIR EE Close the MFCS device and the allocated memory mfcs set purge on Connect the channel 1 output directly to the pressure supply This function should be used with care as it can cause damage to the microsystems connected mfcs set purge off Disconnect the channel 1 output from the pressure supply Channel 1 can be used normally mfcs get purge Get the status of the purge TRUE purge ON FALSE purge OFF mfcs get status Get the status of the MFCS 1 Trouble in the MFCS connections USB or electric alimentation 0 MECS is reset MECS needs to be manually rearmed green switch on the front panel 1 Normal 2 Pressure Supply Overpressure 3 MFCS needs to be manually rearmed after overpressure green switch on the front panel mfcs read chan Get the pressure value mBar and the measure time ms of the specified channel from 1 to 4 or 8 mfcs data chan Get the characteristics of the specified channel Pressure Unity 02 no captor 12 water inch 2 PSI Maximum Pressure range in the specified unity Zero pressure value of the captor unsigned 12 bits Pressure value unsigned 12 bits Chrono value unsigned 16 bits mfcs get serial Get the Serial Number of the MFCS mfcs set auto Set the regulated pressure mBar for the specified channel from 1 to 4 or 8 If the specified channel is 0 the same is applie
28. our device mm Press STOP to stop the pressure Channel 1 Channel 2 Jj 5 00 J Channel S Channel 6 7 Control 2 MFCS Thanks to this Vi it is possible to control several sen Ss MECS at the same time ze ape Ire CHE GT yere Theta O Enter the two serial numbers Z H 7 ees AE sri Eneas O Select the MFCS of interest with the MN 550 00 150 07 31581 em 1 2 switch of the INPUT box 5 Ka O Set channel number pressure order S js appropriate Alpha feedback SS Bn coefficient Press STOP button to stop pressure jo 7 24 FLUIGENT MFCS User Manual Calibration Set pressure and alpha analog Set pressure digital The Calibration process can also be in operated from Labview interface This example allows you to set an order pressure and feedback coefficient and then to read the result on an analog curve This example allows you to set a pressure order and monitor the result on a numerical display 25 FLUIGENT MFCS User Manual 5 4 C and VBA 5 4 1 Function description preliminary Here is a global description of the functions provided with the library mfcs initialisation Initialize the USB for the MFCS If the Serial Number is not specified or equal to 0 the first MFCS device found will be initialized If the Serial Number is specified the MFCS device with this Serial Number is initialized Each MFCS has a unique Serial Number written on the back pa
29. rpressure switch on GREEN button Initialize the MFCS device O lf the serial number is not specified or equal to 0 the first MFCS device found will be initialized If the serial number is specified the corresponding MFCS device is initialized Each MFCS has a unique serial number written on the back panel The VI returns the handle and the serial number of the MFCS 0 if no MFCS found Get the pressure value mBar and the measure time ms of the specified channel from 1 to 4 or 8 23 FLUIGENT MFCS User Manual mfcs set alpha vi Set the alpha value default 5 for the specified channel from 1 to 4 or 8 O f the specified channel is 0 the Handle in Handle out same is applied to all channels Channel number O Alpha is linked to the proportional default all value of the PID ressure Alpha default Error out regulation Error in mfcs set auto vi Alpha default 5 Set the regulated pressure value mBar for the specified channel from 1 to 4 or Handle in Handle out Channel number Ifthe specified channel number is 0 all mme Bressiiretntan d Error out 0 the same pressure is applied to all the channels Error in It is also possible to set the alpha value mfcs set manual vi Set the electrovalve alimentation 96 for Handle in Handle out the specified channel from 1 to 4 or 8 Channel number 1 f the specified channel is 0 the O all Error out same is applied to all channels S
30. ults and compensate for offset drift of the pressure sensors The housing can be cleaned with a moisty soft tissue iso propanol or window cleaning products Avoid aggressive organic solvents as acetone or chloroform or abrasive cleaning products Please use the following form if you have problems or suggestions with your MFCS device or software and send it to us by fax or e mail 35 LA B R NR FL UIGENT MFCS User Manual 6 7 Trace and Log files format MFCScontrol allows a complete real time recording of all settings and measured pressure values during operation LILIUT 2002 17139703 MECS SJ S0903 X P0DO 01 15885 18 424 426 146 20 9100 15886 18 424 920 LU U 20 477 toga L94909 1999 LU U 20 454 1998998 Best 426 puso 202 909 15889 18 485 Mes LOU 20 583 15890 18 462 426 LU U 20 9500 15891 18 402 920 146 204500 199927 1912599 03998 Salz Figure 10 an example of a Trace file The trace file format consists of a header line and a data block The header line is composed of the starting date and time serial number of the connected unit and version number of the MFCScontrol software The data block is composed of lines carrying a time stamp absolute time and the internal step number and the 4 8 pressure values N A means information lost e g transient transmission failure When using this option avoid the creation of oversized data files Adjust the storage frequency according to the needs see pull do
31. view library provided is embedded with a set of functions that will allow you to control the MFCS in your own Labview program Here are a few examples of what you can achieve with this library 5 3 1 Labivew VI Labview VI Symbol mfcs close vi Handle Error in Error out mfcs data chan vi Handle in Handle out Channel data S sl data Error in Error out mfcs get purge vi Handle in Error out Purge ON Error in Handle out mfcs get serial vi Handle in Handle out E Serial Number SSL Error out Handle in Handle out MECS Status Error in Error out mfcs get status vi mfcs initialisation vi Handle in Handle out LS Serial number Error in Error out mfcs_read_chan vi Handle in Handle out Channel numbe t Error out s L Pressure mBar Error in e Measure Time ms Close the MFCS device and the allocated memory Get the characteristics of the specified channel O Pressure unity 02no sensor 1 water inch 2 PSI O Maximum pressure range in the specified unity O Zero pressure value of the sensor unsigned 12 bits O Pressure value unsigned 12 bits Get status of the purge in channel 1 O True Purge ON O False Purge OFF Get the serial number of the MFCS 0 if no MFCS connected Get the status of the MFCS O 0 MFCS is reset MFCS needs to be manually rearmed switch on GREEN button 12 Normal 2 Pressure Supply Overpressure 3 MFCS needs to be manually rearmed ater ove
32. wn menus JUTSADUS LTIZT7TS39 BEGIN OF LOG 07 2008 Lite E AUTO Char 07 200 L722 leas AUTO Chan 072005 17 27 43 AUTO Chan 072008 17527822 AUTO Chan 07 2005 17229723 AUTO Chan 2072009 7227822 AUTO Chan 07 2005 17 27 43 AUTO Chan 072200S 17327545 AUTO Chan 0742008 17527345 AUTO Chan 407 2005 17 27 46 AUTO Chan 07 2005 17 27 46 AUTO Chan 07 2005 17 27 46 AUTO Chan MO7e2000 1723291227 END OF LOG La LA LA A A A KA KA RR EE Figure 11 an example of a Log file 36 Ls FLUIGENT MFCS User Manual ALPHA Chan X YYYYY Order set O value to YYYYY in Channel X AUTO Chan X YYYYY Order set value YYYYY in Channel X in feed back mode BEGIN OF LOG Order start logging END OF LOG Order stop logging MANUAL Chan X YYYYY Order set value YYYYY in Channel X in manual mode RE EEE Table 2 commande sent from the computer to the MFCS mfcs exceeding input pressure Message input pressure exceeds threshold value mfcs operating Message system is operating normall essage comprising serial and version number mfcspurgeon Message purge is switched on m cs reset detected Message system has realized activation of reset signal button processor reset line Table 3 commands sent from the MFCS to the computer 37 VG B R NR FL UIGENT MFCS User Manual Fluigent tel 33 0 15373 1551 fax 1552 MFCS date e mail support fluigent com
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