ATFocus-5 User's Manual 1.2.Book
Contents
1. WD FUNCTIONAL DESCRIPTION g Objective y Above focus focus Specimen Laser beam Pa Below focus Figure 2 1 PRINCIPLE OF OPERATION 2 1 3 ATF 5 Output Characteristics and Digital I O The sensor s output characteristic is internally linearized to match the objective lens easy to use calibration software is provided The distance to focus measurement is always available on the sensor s RS232 communication link Sensors optionally equipped with the analog output also present this information in the form of an analog voltage signal If the specimen is out of sensor range the sensor s output is set to 0 DU 5 volts on the analog output and the n Capture Range DI O signal is low Once the specimen is detected the n Capture Range DI O signal is asserted For as long as the specimen is far from focus the sensor s output remains at 512 DU and the sign of the output indicates the direction towards the In Focus position for sensors equipped with the analog output the voltage is set to either 8 V or 2 V depending on the direction to focus In the vicinity of focus the sensor s characteristic is linear i e the output is proportional to the distance to focus Once the microscope reaches the n Focus position the n Focus DI O signal is asserted The In Focus DI O signal remains high as long as the microscope objective is maintained within its depth of field DOF The width of the DOF can be con2. figured with the software ATFocus 5 User s Manual 2 3 WD FUNCTIONAL DESCRIPTION se A tre Rang eame Linear Fang e i H D1 k Ll L meh m ro a gi in Range D 72 E in Focus DO Depth of il focus Figure 2 2 Sensor s output characteristic and digital I O In addition to the status signals n Capture Range and In Focus the ATF 5 is equipped with two control digital inputs e Laser enable for deactivating the sensor s laser and suspending the auto focusing functions This signal is intended to be incorporated with the laser safety interlock e Camera sync for deactivating the sensor s laser in sync with the microscope s imaging system laser is turned off when the imaging system is exposing ATFocus 5 User s Manual FUNCTIONAL DESCRIPTION WDI 2 1 4 TYPICAL APPLICATION For installation the ATF 5 sensor requires an optical port in the infinity range of the microscope and a customized mounting bracket for attaching the sensor The sensor s output is sent to the auto focus servo controller for automatic adjustment the microscope objective Z position If application assistance is required please contact WEGU DEVICE Inc There are two options to interface a user supplied servo controller to the sensor e using a serial RS232 communication link e using the sensor s analog output and digital inputs and outputs Infinity Tube p tical Port Tube Lens Se
3. nsors Beam sphiter Focus Actuator Objective Figure 2 3 Auto Focus System Components ATFocus 5 User s Manual 2 5
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