BioRad Confocal Troubleshooting Why Can't I See An Image?????
Contents
1. B THE UNIVERSITY OF J WESTERN AUSTRALIA SZ Centre For Microscopy Characterisation and Analysis BioRad Confocal Troubleshooting 1 The Laser is not turned on 2 The doors on the orange safety box aren t ALL closed properly 3 You are in the wrong focal plane try refocusing your specimen 4 The microscope is not on C 5 The microscope knob is not properly clicked into the C position 6 An epifluorescence filter cube is in the laser light path 7 The wrong dichroic filter blocks are in 8 Gain Iris or laser power is not high enough 9 You are clicked on the wrong icon eg FITC instead of TRITC 10 DIC analyser A slider is not pulled out 11 For UV only you are using an objective that does not transmit the UV Use a Fluor objective 12 Objective nosepiece is properly located at a click stop http www cmca uwa edu au instrumentation optical confocal Page 1 of 1
Download Pdf Manuals
Related Search