How do you increase your labs DNA reproducibility
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1. Unlike all other drop based cuvettes the MFC does not need to have two pieces of quartz pressed together to work Simply place a drop on the Micro Chamber and insert it into the Optical Body This provides a leak proof loading system that saves time and money Locked Lightpath Technology Get the consistent results you need with a lightpath that does not change The Locked lightpath does not rely on two plates being compressed to form a seal The MFC has a consistent 1mm lightpath so you know your results will be accurate test after test Try the MFC in your lab risk free For a limited time NSG is offering you the chance to try the MFC 100 free If you are not 100 satisfied with the performance of the MFC simply mail it back to NSG within 2 weeks NSG will even pay the return shipping so you don t have to invest any of your money Lifetime Warranty The MFC comes with a lifetime warranty so if the MFC is damaged in anyway NSG will replace the damaged part s with a brand new one The MFC is the only micro cuvette that is Certified by NRC Canada www microfocuscell com2. the world that has received a certification from NRC Canada for a micro cell Excerpts from NRC Report Wavelength Repeatability Tests We prepared three types of the said above protein solution 1 Concentration 100 2 Concentration 10 3 Concentration 1 Note lab quality distilled water was used as a solvent All solutions produced a very strong sharp wavelength pick in the long UV region of the spectrum Wavelength tolerances in all measurements were within quite tight tolerances of the spectrophotometer we used refer to the spectrophotometer s User Manual Photometric deviation caused by mechanical instability of the spectrophotometer s cell holder did not cause any issues with wavelength repeatability Cross contamination Tests We measured several times the said pick s absorbance with samples of all concentrations mentioned above Then just by touching the top of the elevated cavity of the MFC device with a swab supplied by the NSG Precisions Cells Inc s www precisioncells com we were able to easily remove liquids samples from the said cavity When distilled water was used following a swab s suction of the sample described above the photometric absorption of the residual sample s film was about 0 2 AU which is within the baseline noise discussed above Important any our attempts to further clean the MFC s cavity with the concentrated bleach did not result in any additional reduction of the phot
3. How do you increase your labs DNA reproducibility by in your experiments Here you go again Filling that old 10 mm cuvette with a giant amount of test solution D You carefully place the cuvette down and you turn to sip your coffee All of a sudden your arm grazes the cuvette and you spill that expensive DNA sample all over the work area Micro Focus Cell Design The Micro Focus Cell is the only ultra micro cuvette that utilizes a two part design The first part is the Optical Body OB The OB houses the highly polished micro focusing lenses These lenses send the spectrophotometer s beam directly through the sample while keeping the optical beam unaltered The OB has the same dimensions as a standard 10 mm cuvette 12 5 x 12 5 x 455 mm The second part of the Micro Focus Cell is the Micro Chamber MC The Micro Chamber is where the sample is placed The MC is removable from the OB so a sample can be loaded either in the spectrophotometer or on the workbench The design of the MC is very sophisticated You can knock it over and the sample will stay safely in place Using the Micro Focus Cell When using the MFC the only tools required are a spectrophotometer and a micro pipette Simply take a 1 9 ul drop of solution place it on the Micro Chamber and slide the MC into the Optical Body Run the test clean and you re ready for another sample The whole process takes less than one minute Hydrodynamic Cleaning
4. amp Cross Contamination One of the areas where other drop based cuvettes fall short is in cleaning All other designs use two plates to compress the sample in the holder What happens is that when users accidentally use too much sample they end up having the sample leak out of the cuvette when the plates are assembled This causes many problems with cross contamination and cleaning The MFC s Micro Chamber uses one piece of high grade ES Quartz to hold the sample The sample rests directly in the quartz chamber and there are no other pieces to assemble Once you place the sample in the holder all you need to do is insert the MC into the Optical Body To clean you simply place a laboratory grade cleaning swab on top of the MC The sample is quickly cleaned within seconds Samples can also be retrieved by using a micro pipette www microfocuscell com One Touch Cleaning Illustrated below is how quick and easy it is to clean the quartz chamber of the MFC In three quick and easy steps you are ready to run your next sample with zero cross contamination Cross Contamination Cross contamination was tested by NRC Canada and they verified that the cross contamination spec for the Micro Focus Cell is 3 5 au In simple numbers this translates to 0 0001 or 1 10 000th of the samples transmittance value is effected by the cross contamination of the MFC All that remained of the previous sample was 0 0001 The Micro Fo
5. cus Cell is the only drop based micro cell that does not require concentrated bleach for cleaning Simply using distilled water reduces the cross contamination to zero Perfect Alignment Equals Perfect Reproducibility In the past the Hellma TrayCell was a great drop based micro cuvette The design used a series of mirrors to direct the optical beam through the sample which was at the top of the cell and then back down to the spectrophotometer s detector This design has some limitations If you did not align the cell 100 correctly with the beam the cell would not work The TrayCell was only recommended for a few machines and was not guaranteed to work in all spectrophotometers NSG s MFC eliminates all issues that are caused by misalignment The first use of the MFC does require a quick alignment procedure and since the Optical Body stays in the spectrophotometer no further adjustments are required The alignment procedure consists of inserting the OB and MC together into the spectrophotometer Then choose either Baseline 0 or Baseline Correction and you are done WWW PRECISIONCELLS COM Hydro Grip Technology Have you ever spilt a sample from a cuvette before Now with NSG s Hydro Grip Technology you will never spill another sample again Hydro Grip Technology is the system we designed that keeps the sample in place no matter what direction you turn the Micro Chamber By fine tuning
6. ive you increased productivity in your daily experiments or if you re not completely satisfied with it just let us know within 14 days and simply send the cell back to us We ll even pay the shipping cost Say yes to this offer and we will put the power of a Micro Focus Cell in your hands 10 11 Top 11 Reasons for You to Buy a Micro Focus Cell Now Best reproducibility for your experiments The MFC features a Locked Lightpath as well as perfect alignment eliminating all possible variables Easy for you to load your sample The MFC provides you with flexibility to load your sample in or outside your spectrophotometer You ll never spill or waste a sample The Hydro Grip Technology of the Micro Chamber allows you the ability of turning the MFC upside down or you can toss the Micro Chamber to a colleague across the room and the sample will stay safely in place Guaranteed to work in your spectrophotometer NSG guarantees that the MFC will work in any spectrophotometer you own ZD is adjusted using the black inserts that come with the MFC Perfect alignment for more control of your experiment The MFC Optical Body can stay inside the spectrophotometer giving you the same alignment for each test You can clean the cell in seconds with no carry over The One Touch Hydro dynamic cleaning system sucks the sample out of the cell with 3 seconds when using one of NSGs cleaning swabs The MFC is easy for you to use
7. ometric absorption which demonstrated no need of using bleach cleaning for the MFC device within the scope of our evaluation www microfocuscell com Other We never incurred any leakage of liquid samples from the MFC s cavity Technical Specifications NSG Part Number MEFC 1 Outer Dimensions mm 12 5 x 12 5 x45 Lightpath mm 1 0 Minimum Sample Volume ul 1 9 DNA Detection Limit ng ul 0 5 DNA Max Concentration ng ul 1 800 DNA Reproducibility at 100 ng ul 0 4 DNA Reproducibility at 1000 ng ul 1 8 Protein Detection Limit mg ml 0 025 Protein Reproducibility at 1 mg ml 0 004 Protein Reproducibility at 10 mg ml 0 001 Protein Maximum Concentration 110 mg ml Notes Technical performance determine using a biopharm grade double beam UV VIS spectrophotometer with a 2nm spectral bandwidth Xenon lamp BSA determined with A280 direct UV method Maximum concentration is limited by a specimen solubility Lifetime Warranty NSG Precision Cells has become well known for offering Lifetime Warranties on select exclusive items The Micro Focus Cell comes with a Lifetime Warranty so if the MFC is damaged in anyway NSG will replace the damaged cell with a brand new one Try One Risk Free NSG needs you to stop wasting your time and money on other cuvettes You too can increase the efficiency in DNA experiments in your lab by using a Micro Focus Cell Try a Micro Focus Cell now risk free If the MFC doesn t g
8. ot 100 accurate the sample can leak out due to the compression Wavelength Stability Wavelength stability is the ability of a micro cell to measure peaks accurately test after test Wavelength peaks are dependent of the purity of the sample used for an experiment The Micro Focus Cell offers the following features to ensure there are no shifts in the wavelength peaks 1 The beam entering the cell does not get altered from the beam exiting the cell In other words the beam remains unchanged by the MFC 2 The angular inclination of the beam is unchanged when using the MFC Photometric Absorbance The Micro Focus Cell is the only drop based cell that uses a sequence of Fresnel lenses in the optics design The largest lens used has a diameter of 6 mm Using a lens with such a large diameter gives users the power of harnessing 50 of the light reaching the spectrophotometers detector This large amount of light reaching the detector is not changed by the angular position of the cell Applications for the Micro Focus Cell DNA Quantification RNA Measurement DNA Measurement Nucleic Acid Analysis Protein Concentration Linearity Data for what is the sample Sample Q06 a oog o Qos 0 c2 Q01 1 sol at 00 20 10 ol ste 09020 100 sol ett OD 2 0 275 280 www microfocuscell com Certified by NRC Canada NSG Precision Cells is proud to be the only company in
9. the dimensions of the quartz chamber we have made it virtually impossible for a user to spill a sample once it has been placed into the Micro Chamber You can knock the Micro Chamber over and the sample will stay in place What Z Dimension do you have There are a large amount of spectrophotometers on the market All people buying cuvettes will already own a spectrophotometer There is nothing more frustrating than buying a cuvette and then finding out that the Z Dimension is incorrect When purchasing a Micro Focus Cell you don t need to worry about dimensions NSG has ensured that the MFC is compatible with all spectrophotometers You can have a 8 5 15 or even 20 mm ZD One Micro Focus Cell will work in all of them NSG is the only company that guarantees that the Micro Focus Cell will work with any spectrophotometer with a 10 mm cell holder www microfocuscell com Advanced Optics System The Micro Focus Cell uses a sequence of highly polished Fresnel lenses These special lenses guide the optical beam directly through the sample The alignment of the MFC is very easy as long as the beam is in line with the 4 mm round window see left photo Once the optical beam comes into contact with the round window the beam is sent through the Micro Chamber and to the spectrophotometers detector We believe in keeping the optics as simply as possible The MFC does not use any mirrors prisms or other optics Multiple Loading Config
10. urations When designing the MFC NSG had one goal at the top of their list which was ease of use NSG needed to meet the needs of the most demanding conditions so we designed the MFC to provide you with maximum flexibility Samples can be loaded in by keeping the Micro Chamber in the Optical Body or by removing the MC If for some reason your sample is in the lab down the hall simply take the MC with you It cannot be any easier Locked Lightpath What guarantees consistent results That s easy keeping all variables the same test after test All other micro cells use a method of pressing two plates together to make a seal Using this method a user is able to easily insert the sample on the cell The problem with this is that the lightpath can be different for every measurement This results in inconsistent results and does not provide the accuracy that you need for DNA tests The MFC is the only drop based cuvette that has a Locked Lightpath of 1 mm This lightpath never changes because the quartz optics on the micro chamber is made of one solid piece The sample is www microfocuscell com placed directly into this chamber There is no need to assemble or disassemble the sample holder Once the sample has been placed in the Micro Chamber you only need to insert it into the Optical Body Another benefit of this design is that you will not waste one drop of your sample When using the pressing plates design if you are n
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