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The MICROLAB® 500 Series:

1. T a S st me C vme o oC SE ee Hand Probe Connector 2 3 Receptacle i H m E L te Step Prime Ka D Switch a D Power Indicator Light PEN Power l A On Off 7 Switch 1 10 MICROLAB 501A 503A and 504A User s Manual Figure 1 7 shows the front view of the MICROLAB 504A dual syringe dispenser In this figure the controller unit rests on top of the drive unit and syringes are attached to the syringe drive arms Figure 1 7 Front View of the MICROLAB 504A Dual Syringe Dispenser 25u f NECE o Corer K eiere 2 DZ MICROLAB SOO rene d Hand Probe Connector Receptacle Step Prime Switch Power Indicator Light Power On Off Switch Chapter 1 Getting Started 1 11 Figure 1 8 shows the r
2. Controller with memory e Microlab 530B diluter dispenser e Microlab 540B dispenser Controller with memory e Microlab 510B diluter dispenser Computer controlled e Microlab 531C diluter dispenser e Microlab 541C dispenser Computer controlled e Microlab 511C diluter dispenser The MICROLAB 500 Series of instruments consists of three different lines of diluter dispensers e Controller without memory Use the controller unit to manually enter methods This controller unit does not have memory so methods cannot be stored PR 2 MICROLAB 501A 503A and 504A User s Manual Controller with memory This controller unit has memory allowing you to program and store your own custom methods Or use the controller unit to run manual methods Computer controlled No controller unit is included use a computer to operate the instrument The MICROLAB 501A 503A and 504A are described in this manual The MICROLAB 501A is a single syringe dispenser designed for single precision dispensing applications The MICROLAB 503A is a dual syringe diluter designed for repetitive dilution applications The MICROLAB 504A is a dual syringe dispenser designed for precision dispensing applications that require more than one liquid to be dispensed at a time Upgrading Your MICROLAB 500 System Both the MICROLAB 501A and 503A systems can be upgraded
3. Table 1 4 Tubing Kit 35887 For All Models 12 ga x 650 mm 18 ga x 650 mm 12 ga x 900 mm 18 ga x 900 mm Fill Tubing not Fill Tubing not Dispense Tubing Dispense Tubing tapered tapered tapered tapered Part Part Part Part 240000 240010 240360 240130 Figure 1 4 Tubing Kit items shown are not to scale 12 ga Fill Tubing Part 240000 12 ga Dispense Tubing Part 240360 18 ga Fill Tubing 18 ga Dispense Tubing Part 240010 Part 240130 M6 threaded hubs are used on all tubing For complete lists of syringes accessories and replacement parts for the MICROLAB 500 see Appendix C Ordering Parts and Accessories for the MICROLAB 500 Chapter 1 Getting Started 1 7 A Brief Introduction to the MICROLAB 500A The MICROLAB 501A 503A and 504A systems each consist of three basic units These units include e adrive unit e a controller unit e ahand probe This section briefly describes these units and the individual components that comprise each unit See Chapter 2 Installing the MICROLAB 500 for complete installation instructions see Chapter 3 Using the MICROLAB 500 for complete usage instructions Drive Unit The drive unit is the heart of each MICROLAB 500 system The drive unit contains a precision drive motor the syringe drive arms the valve assembly and the power switches and connector receptacles These features allow you to control other
4. See Appendix C for information regarding replacement parts for TLL TLLX and DX syringes Figure 2 5 The TLL type Dispenser Diluent Syringe uda aa fall l Figure 2 6 The TLLX type Dispenser Diluent Syringe A LLL o Figure 2 7 The DX type Sample Syringe q ILI LU UA l Chapter 2 Installing the MICROLAB 500 System 2 9 Preparing Syringes for Installation When you unpack a new syringe notice that the syringe plunger is packed separately from the syringe barrel You must condition the plunger tip before inserting the plunger into the barrel A Important Before using a new syringe for the first time you must condition the syringe s PTFE plunger tip and glass barrel To condition the tip and barrel wet the plunger tip with distilled water or a solvent Medical type silicone lubricants may be used to extend the lives of PTFE plunger tips However silicone may contaminate the plunger tips Do NOT use viscous oils to lubricate plunger tips After wetting the plunger tip insert the plunger into the glass barrel Move the plunger in and out of the glass barrel approximately 10 times Apply steady and even pressure avoid twisting movements A Installing Syringes Follow these steps to install syringes on any
5. D 2 MICROLAB 501A 503A and 504A User s Manual continued Table D 1 Chemical Compatibility continued Solvent PTFE Kel F Freon 1 Gasoline Glycerin Hydrochloric Acid Hydrochloric Acid conc Hydrofluoric Acid Hydrogen Peroxide Hydrogen Peroxide conc Hydrogen Sulfide Kerosene Methyl Ethyl Ketone MEK Methyl Alcohol Methylene Chloride e tri ojrmrilio jo o o ro o o Naphtha Nitric Acid Nitric Acid conc Nitrobenzene P a e Phenol Pyridine Silver Nitrate Soap Solutions Stearic Acid OJLL G LO TT S e o oyo ro o O I OO O O G Sae S O Appendix D Chemical Compatibility of the MICROLAB 500 continued D 3 Table D 1 Chemical Compatibility continued Solvent PTFE Kel F Sulfuric Acid 0 Sulfuric Acid conc Sulfurous Acid Tannic Acid 0 0 0 ie Jolo Tanning Extracts Tartaric Acid Toluene j Triclorethylene Turpentine Water Xylene O OD E E S o N O o w D 4 MICROLAB 501A 503A and 504A User s Manual Glossary active valve A valve that allows for diversion of liquid flow from the input side to a syringe and from a syringe to the output side air gap A segment of air that is aspirated through the probe used to separate different liquids in the tubin
6. 1 18 MICROLAB 501A 503A and 504A User s Manual Press the left Volume key to select the left syringe Then press the Increase or the Decrease key to change the volume to be pipetted by the left syringe Press the right Volume key to select the left syringe Then press the Increase or the Decrease key to change the volume to be pipetted by the right syringe When you select either the left or the right Volume key a green LED indicates which of the keys is active Note The message display windows show the volume settings in microliters Speed Keys To set the speed of a syringe drive arm press either the left or the right Speed key then press the Increase or the Decrease key You must enter a syringe size before you can change its speed dll Baan Press the left Speed key to select the left syringe Then press the Increase or the Decrease key to change the speed of the left syringe drive arm Press the right Speed key to select the right syringe Then press the Increase or the Decrease key to change the speed of the right syringe drive arm When you select either the left or the right Speed key a green LED indicates which of the keys is active Note Drive arm speeds represent the number of seconds needed to drive a syringe full stroke 6 cm Therefore the smaller the speed number the faster the speed The fastest speed is two seconds while the slowest speed is 20 seconds Chapter 1
7. 39111 Reagent Bottle Holder 35783 Accessory Holder also known as a MICROLAB Probe Accessory Bracket needed with reagent bottle holder 16500 Screw 4 40 x 7 8 two required to attach bracket 88990 Tubing Clips 5 pack 18310 Hamilton Syringe Cleaning Solution 70 cc 18311 Hamilton Syringe Cleaning Solution 500 cc 1524 01 Fuse Slow Blow 1 amp 6541000 Power Cord 115 V Three prong Grounded 355010 Power Cord 220 V Schuko continued Appendix C Ordering Parts and Accessories for the MICROLAB 500 C 3 Table C 6 Parts and Accessories continued Part Number Description 69175 MICROLAB 501A 503A 504A User s Manual English version 69181 MICROLAB 501A 503A 504A User s Manual French version 69179 MICROLAB 501A 503A 504A User s Manual German version 69185 MICROLAB 501A 503A 504A User s Manual Portuguese version 69187 MICROLAB 501A 503A 504A User s Manual Spanish version For more information about ordering parts and accessories or upgrading your MICROLAB 500 system contact your authorized Hamilton dealer or the Hamilton Company In the United States Hamilton Company P O Box 10030 Reno Nevada 89520 0012 Telephone Numbers in the USA and Canada Technical Customer Service 1 800 648 5950 8 a m to 5 p m PST Instrument Service 1 800 527 5269 Outside the USA and Canada 1 775 858 3000 Fax Number 1 775 856 7259
8. In Switzerland Hamilton Bonaduz AG Ch 7402 P O Box 26 Bonaduz Switzerland Telephone Number 41 81 660 60 60 Fax Number 41 81 660 60 70 Cc 4 MICROLAB 501A 503A and 504A User s Manual Appendix D Chemical Compatibility of the MICROLAB 500 The following table contains information about chemical reactivity with the MICROLAB 500 system Legend No data available 0 No effect excellent 1 Minor effect good 2 Moderate effect fair 3 Severe effect not recommended Table D 1 Chemical Compatibility Solvent PTFE Kel F Acetaldehyde 0 0 Acetates Acetic Acid Acetic Anhydride o oOo o o Acetone Acetyl Bromide Ammonia Ammonium Hydroxide Ammonium Phosphate Ammonium Sulfate oO fjo o o o o o o o o Fl o o o o Amyl Acetate continued Table D 1 Chemical Compatibility continued Solvent PTFE Kel F Aniline 0 Benzene Benzyl Alcohol Boric Acid Bromine Butyl Alcohol Butyl Acetate Carbon Sulfide Carbon Tetrachloride Chloracetic Acid Chlorine Chlorobenzene Chloroform e e li OoOl jrRFioqoyjynio o lo yotl n Chromic Acid Cresol Cyclohexane Ethers Ethyl Acetate Nie JJe lejo Ethyl Alcohol Ethyl Chromide Formaldehyde Formic Acid OIO O OO JwT GOG e SNG N SGO OS O a N OG GG NO A S
9. Figure 2 13 Figure 2 14 Table 2 1 Table 2 2 Table 2 3 Table 2 4 Overview of Installation Procedures 0666 2 2 Installing Electrical Connections cccceeeeeeee 2 5 Installing a Valve Assembly on the MICROLAB 501A 1 ccecccccseeeeeeeeeeeeeeaaeeeeeaaeneeees 2 6 Installing a Valve Assembly on the MICROLAB 503A ANG 504 Anane intimidate E 2 7 The TLL type Dispenser Diluent Syringe 2 9 The TLLX type Dispenser Diluent Syringe 2 9 The DX type Sample Syringe 2 9 Installing a Syringe 0 0 eects eeeeee eee eeeeeeeeeeeeeeeaes 2 11 Removing a SYFiNGE ceeeeeeeeeeeeeeeeeteeeeeeeeeeeaee 2 13 MICROLAB 501A Valve and Tubing Connections ccceeeececeeeeeeeeeeeeeesees 2 16 MICROLAB 503A Valve with a D Configuration SYFINQE Hii hie twwi ahi wala bares ert 2 17 MICROLAB 503A Valve with Two TLLX or TLE Shines 2 04 sie ck ae as 2 17 MICROLAB 504A Valve and Tubing COnnectors cccceceeceeeeeeeeeeeeeaeeaeeeeeees 2 18 Using the Tubing Reducer with the Dual Hand PROD Gi ezakictd coat oe gett ale teeter oan 2 19 Language Selection Switch Positions 2 4 Reagent Diluent Syringes 2 8 Sample Syringes cccceeeeeeeeeeeeeeeeeeeeeeaeaaeeees 2 9 Tubing Selection Guide cccceeeeeeeeeeeeeeees 2 14 Chapter 3 Chapter 5 Appendix A Appendix B Appendix C Figure 3 1 Figure 3 2 Figure 3 3 Figure 3 4 Figure 3 5 F
10. Press the left or the right Volume key When you press the Volume key the Control Indicator a small green light next to the key lights up to show you that the key is active By default volume appears as 100 of the corresponding syringe 100 is the maximum allowable volume If you enter a volume of 0 zero the corresponding syringe remains inactive Enter a volume greater than zero to activate the syringe To increase or decrease a volume press the Increase or Decrease key You can select any volume in increments of 0 1 of the corresponding syringe As you press the Increase Decrease key the display changes continuously When either the highest or the lowest volume limit is reached the display automatically wraps around enabling you to quickly select the correct volume for your needs When you reach the volume you need release the Increase or the Decrease key Verify your selection by checking the volume that appears in the message display windows Note If you enter a new size for a syringe volume automatically changes to 100 of the new size If you are using a dual syringe instrument repeat steps 1 through 4 for the second syringe MICROLAB 501A 503A and 504A User s Manual Setting Syringe Drive Speeds To change the speed of a drive arm follow these steps 1 Press the left or the right drive arm Speed key by pressing it When you press a Speed key the Control Indicator a small green light nex
11. This equipment has been verified to comply with the limits for a class B computing device pursuant to FCC Rules In order to maintain compliance with the FCC regulations shielded cables must be used with this equipment Operation with non approved equipment or unshielded cables is likely to result in interference to radio and TV reception The user is cautioned that changes and modifications made to the equipment without the approval of the manufacturer could void the user s authority to operate this equipment Contents Figures and Tables ccccceee cece cece eee eeeeeeeeeneeeeeneeeeneeeneenees ix Preface oinaren rannen EEA NEAVAND ENEE O AAAA PR 1 About the MICROLAB 500 Series of Instruments PR 2 Upgrading Your MICROLAB 500 System c cceceseeeeees PR 3 About This Manual enara a PR 4 Conventions Used in This Mantal cceeeeeeeeeeeeeeeeeeeaes PR 5 A Word About Single Syringe Instruments ceeeereee PR 5 Chapter 1 Getting Started c ccc ce cece eee eee e ee eee i iia 1 1 MICROLAB 500 Parts Lists eeaeee nenne aenean anneren nena 1 2 A Brief Introduction to the MICROLAB 500A ceeeeeeeee 1 8 BEA i E E A E T EEA O O E duvtect weer 1 8 Power Cord Connector Receptacle ccccsssseseeeeeeees 1 13 Hand Probe or Footswitch Connector Receptacle 1 13 FUSE BOX ceranae ivi ean ay ented av avanee ae geese 1 13
12. Use this probe with MICROLAB 501 and 503 systems The Concorde probe attaches directly to a sampling syringe or to the valve assembly This probe accommodates both 12 or 18 gauge tubing and features adjustable extension lengths beyond the probe tip See Figure 1 10 Dual Push button Hand Pipettor Probe The Dual Push button Hand Pipette Probe is a dual dispense pipette that features a pistol grip design with push button actuator Use this probe with MICROLAB 504 systems This probe accommodates both 12 and 18 gauge tapered tubing lines with independent extensions You can extend the tubing out the end of the probe to the length that is convenient for your application either the right side or the left side tubing can be extended to pick up sample Figure 1 10 shows the Dual Push button Hand Pipette Probe 1 22 MICROLAB 501A 503A and 504A User s Manual Disposable Tip Push button Hand Pipettor Probes The Disposable Tip Push button Hand Pipette Probe is a single dispense tube pipette that features disposable tips and push button tip ejection The Disposable Tip Push button Hand Pipette Pipettor Probe is an optional accessory Use this probe when sample to sample carryover is a concern See Figure 1 10 Figure 1 10 shows the Concorde the Disposable Tip and the Dual Hand Pipettor Probe Figure 1 10 The Concorde the Disposable Tip and the Dual Hand Pipettor Probes ly O Concorde Disposable Tip Dual Hand Pipettor P
13. 80926 1705DX 10437 N A 1117 01 N A N A N A Syringe 100 uL 81026 1710DX 10438 N A 1117 02 N A N A N A Syringe 250 uL 81126 1725DX 10439 N A 1117 03 N A N A N A Syringe 500 uL 81226 1750DX 10440 N A 1120 01 N A N A N A Syringe 1 0 mL 81326 1001DX 10441 N A 1578 01 N A N A N A Syringe Table C 3 Valve Assemblies Part Number Description 35825 HV Valve Model 501A 35844 Diluter Valve Model 503A 35842 Dispenser Valve Model 504A Table C 4 Tubing Part Number Description 240000 Fill tubing 12 gauge 650 mm with Fittings 240010 Fill tubing 18 gauge 650 mm with Fittings 240360 Dispense tubing 12 gauge 900 mm with Fittings 240130 Dispense tubing 18 gauge 900 mm with Fittings C 2 MICROLAB 501A 503A and 504A User s Manual Table C 5 Pipettors Probes Part Number Description 35529 Concorde Push button Hand Pipettor Probe Single Channel 35767 Dual Dispense Push button Hand Pipettor Probe 77006 Disposable Tip Push button Hand Pipettor Probe 22 gauge x 900 mm tubing 1 35 uL 77007 Disposable Tip Push button Hand Pipettor Probe 22 gauge x 900 mm tubing 1 125 uL 35899 Luer Lock Tip Push button Hand Pipettor Probe 35898 Viscous Sample Push Button Hand Pipettor Probe 35770 Tubing Reducer 18 gauge for Dual Dispense Push button Hand Pipettor Probe 1 each 77004 Footswitch Table C 6 Parts and Accessories Part Number Description
14. Getting Started 1 19 Operation Keys The operation keys are located in the lower left corner of the controller unit When you select an operation a green LED next to the operation key lights up To start an operation press the Run Stop key or press the probe button When an operation starts the system beeps once The Down operation moves the syringe drive arms down from the V Home position pulling a specified volume of liquid into the syringes e In the MICROLAB 501A dispenser or the MICROLAB 503A diluter dispenser liquid is pulled in from the left side inlet valve port e Inthe MICROLAB 504A dispensers liquid is pulled in from both the left and right outside ports When the Down stroke is complete this could be at any location within the drive arm s range of movement depending on your specific application the operation automatically switches to Up The LED automatically indicates that the Up operation is active The Up operation moves the syringe drive arms up forcing a AAN specified volume of liquid out of the syringes and through the dispense tubing When the Up stroke is complete and the syringe drive arms both arms if using a dual syringe instrument return to the Home position the operation automatically switches to the Down operation The LED automatically indicates that the Down operation is active If both arms do not return to the Home position the Up operation remains active The Auto Refill operation
15. Language Selection Switch ccceceeeeeeeeeeeeeeeeeeeeeeaee 1 13 Controller Unit Connector Receptacle 1 14 Power On Off Switch and Power Indicator Light 1 14 Step Prime SWitCh 0 0 cccceceeee eee eeeeeeeeeeeeeeeaaeaeeeeeeeeeeeeaee 1 14 Valve ASSOMDIY eeen ieor ee EI rE caleenenetveatuersivertectvens 1 15 Syringe Drive AIMS 00ccceeeeeeeeeeeeeeeeeeeeeeeeaeaaeeeeeeeeeeeeaea 1 15 Chapter 2 vi The Controller Unit ccc cece eee eeeeseeeneeeeeueeeeeaeeaeeaeeaees 1 16 Message Display Windows ccceeeeeeeeeeeeeeaaeeeeeaaee ees 1 17 Function Control KeyS 0 ccceeeeeeeeeeeeeeeeeeeeeeeeaeaaaaeeeeeeees 1 17 Increase and Decrease Keys cceeeeeneeeeeeaeeeeeeeaaeeeees 1 17 Syringe Size Keys cceeeeeeeee ee eeeeeeeeeeeeeaaeaeeeeeeeeeaeaaed 1 18 Volume Keys aieeaa a a 1 18 SPCOd Keys iatera a ra a aaia aias 1 19 Operation Keysi aeiaai onni ean aed 1 20 RUNM Stop Key cccceeeeeeeeeee cece eee eeeeeeeeeeeeaeaaaaeeeeeeeeeeaeaaea 1 21 Pipettors Probes ei owe eee veces 1 22 Concorde Push button Hand Pipettor Probe 05 1 22 Dual Push button Hand Pipettor Probe eeeeeeeeeee 1 22 Disposable Tip Push button Hand Pipettor Probes 1 23 Luer Lock Tip Push button Pipette Hand Pipettor Probe 1 23 Viscous Sample Push button Hand Pipette PIREO PIO DG sins faved causes AEE AAE AA 1 24 FOOLS WILG I aae eae tiie heigl
16. Part MICROLAB ML501115 35895 35894 69175 English 6541000 35889 501A 115V 69181 French 69179 German 69185 Portuguese 69187 Spanish MICROLAB ML501220 35895 35894 same as above 355010 35889 501A 220V MICROLAB ML503115 35896 35894 same as above 6541000 35790 503A 115V MICROLAB ML503220 35896 35894 same as above 355010 35790 503A 220V MICROLAB ML504115 35897 35894 same as above 6541000 35791 504A 115V MICROLAB ML504220 35897 35894 same as above 355010 35791 504A 220V 1 2 MICROLAB 501A 503A and 504A User s Manual Figure 1 1 MICROLAB 500A System Components items shown are not to scale C D MICRAAB 5001010 a id Controller Unit and Cord A Part 35894 lt lt f Dual Drive Unit Part 35896 or 35897 Microlab 503A Microlab 504A Power Cord Part 6541000 or 355010 Shipping Kit Part 35889 Microlab 501A Part 35790 Microlab 503A Part 35791 Microlab 504A j Single Drive Unit Z Part 35895 Manual Microlab 501A Part 69175 Chapter 1 Getting Started 1 3 Table 1 2 lists the components that make up the MICROLAB 500 Shipping Kit shown as a box in Figure 1 1 Figure 1 2 shows these components Table 1 2 MICROLAB 500 Shipping Kit Shipping Kit Valve Part Hand Probe Part Small Parts Kit Tubing Kit Part Part Part MICROLAB 501A HV Valve Concorde
17. Probe 35888 35887 35889 35825 35529 MICROLAB 503A Diluter Valve Concorde Probe 35888 35887 35790 35844 35529 MICROLAB 504A Dispenser Valve Dual Hand Probe 35888 35887 35791 35842 35767 2 items 1 4 MICROLAB 501A 503A and 504A User s Manual Figure 1 2 MICROLAB 500 Shipping Kit Components items shown are not to scale A A Dm Small Parts Kit Part 35888 Concorde Probe Part 35529 Microlab 501A Microlab 503A Tubing Kit Part 35887 Dispenser Valve Dual Hand Probe Part 35842 Part 35767 Microlab 504A a ANE 5 Diluter Valve P 35844 Microlab 501A Ei Chapter 1 Getting Started 1 5 Table 1 3 lists the components that make up the MICROLAB 500 Small Parts Kit shown as a box in Figure 1 2 Figure 1 3 shows these components Table 1 3 Small Parts Kit 35888 For All Models Tubing Clips 1 AMP Fuses Accessory Screws Tubing Part Part Holder Part Reducers Part Part 230010 1524 01 35783 16500 35770 2 items 2 items 1 item 2 items 2 items Figure 1 3 Small Parts Kit items shown are not to scale 2 Tubing Clips Part 230010 Accessory Holder Part 35783 Tubing Reducers Part 35770 2 Screws Part 16500 2 Fuses Part 1524 01 1 6 MICROLAB 501A 503A and 504A User s Manual Table 1 4 lists the components that make up the MICROLAB 500 Tubing Kit shown as a box in Figure 1 2 Figure 1 4 shows these components
18. Selecting Tubing Each MICROLAB system includes two gauges of fill and dispense tubing 12 and 18 gauge Fill tubing has a 90 cut on the end opposite the fitting dispense tubing has a tapered end opposite the fitting When selecting tubing consider the volume and the viscosity of the liquid you plan to pipette Refer to Table 2 3 Tubing Selection Guide for help in selecting tubing Table 2 4 Tubing Selection Guide Syringe Tubing Gauge for Tubing Gauge for Size Standard Aqueous Viscous and Solutions Foaming Liquids 25 uL 18 18 50 uL 18 18 100 uL 18 18 250 uL 18 18 500 uL 18 18 ITmL 18 18 2 5 mL 18 12 5mL 12 12 10 mL 12 12 25 mL 12 12 2 14 MICROLAB 501A 503A and 504A User s Manual Note If you have a MICROLAB 503A diluter and plan to use syringes of two different sizes choose tubing to accommodate the syringe with the larger volume See Appendix C for information about ordering extra tubing Installing Tubing 1 Before installing tubing lines wet the tubing fittings 2 Make sure the tubing lines do not contain crimps or blockages Crimps or blockages can cause leaks or can cause the system to overload If tubing is crimped replace it with new tubing A Important Fasten tubing so it is finger tight and snug enough to prevent leaks Avoid using tools to tighten tubing Excessive tightening may damage tubing or valve fittings or flanges A 3 Install t
19. Tables Preface Chapter 1 Figure PR 1 Figure 1 1 Figure 1 2 Figure 1 3 Figure 1 4 Figure 1 5 Figure 1 6 Figure 1 7 Figure 1 8 Figure 1 9 Figure 1 10 Table 1 1 Table 1 2 Table 1 3 Table 1 4 The MICROLAB 500 Series of Instruments PR 2 MICROLAB 500A System Components 1 3 MICROLAB 500 Shipping Kit Components 1 5 Small Parts Kitisisc tscoperheeoebaseidednedhentonehtecterteontadets 1 6 TUBING Alt eresie rE E RASES 1 7 Front View of the MICROLAB 501A Single Syringe DISPENSE ls ecicas earings Jose ites dead eeddcebal aes cueieva abate 1 9 Front View of the MICROLAB 503A Dual Syringe DO a ete rae ner area gt 1 10 Front View of the MICROLAB 504A Dual Syringe DISPENSEF test wek nt sctene ieee ae 1 11 Rear View of the MICROLAB 501A 503A and D04A visa Sea aie eee ee 1 12 The Controller Unit 00 cccecceeeeeeeeeeeeeeeneneeeeeees 1 16 The Concorde the Disposable Tip and the Dual Hand Pipettor Probes cccceeeeeenneeeeeaaeeeeeeaeeeeeeaneees 1 23 MICROLAB 500 System Descriptions 00 1 2 MICROLAB 500 Shipping Kit eeee 1 4 Small Parts Kit 35888 00 c cceeceeeeeeeeeeeteeee 1 6 Tubing Kit 35887 ccccccccceeceseeeeectteeteeeteeees 1 7 Chapter 2 Figure 2 1 Figure 2 2 Figure 2 3 Figure 2 4 Figure 2 5 Figure 2 6 Figure 2 7 Figure 2 8 Figure 2 9 Figure 2 10 Figure 2 11 Figure 2 12
20. chemical compatibility of 4 5 D 1 to D 4 cleaning exterior of 4 4 fluid path of 4 2 syringes and tubing 4 3 when to clean 4 2 performance test reports B 1 to quality control testing B 1 servicing 5 9 5 10 storing 4 5 L language selection switch 1 13 message display windows description of 1 17 messages audible 5 4 code guide 5 2 to 5 4 troubleshooting 5 5 MICROLAB 500 system descriptions PR 2 MICROLAB 504A using as a single dispenser 2 19 O operation running 3 9 to 3 10 operation keys description of 1 20 overview of the MICROLAB 500 series of instruments PR 3 P pausing an operation 3 10 power cord connector receptacle 1 13 2 4 power on off switch 1 14 3 3 preparing syringes for installation 2 10 prime mode description of 1 15 priming the system 3 4 probe button functions 1 24 3 10 probes 1 22 to 1 24 R recovering from errors 3 10 removing syringes 2 12 to 2 13 returned goods authorization number 5 10 Run Stop description of 1 21 running an operation 3 9 IN 2 MICROLAB 501A 503A and 504A User s Manual S selecting installation location 2 3 languages 1 13 2 4 tubing 2 14 setting syringe volumes 3 8 single syringe instruments references to PR 5 speed size keys description of 1 19 step mode 1 15 step prime switch description of 1 14 syringe drive arms 1 15 speeds setting 3 9 syringe size keys
21. laboratory s safety procedures if you use the MICROLAB 500 system to handle hazardous materials A The MICROLAB 500 housing is moderately inert to chemical exposure However some chemicals may discolor the surface of the unit If a spill occurs on the instrument s exterior wipe the surface immediately Wash the surface using a damp cloth with water and soap or use a SANI CLOTH Then dry the area Take care that liquids do not enter the interior of the MICROLAB 500 To disinfect the exterior surface wipe it down with a 10 chlorine bleach and deionized water solution Then dry the area Note The cleaning guidelines presented here are meant as guidelines only If your particular applications require different cleaning solutions contact Hamilton Company for more information 4 4 MICROLAB 501A 503A and 504A User s Manual Chemical Compatibility The MICROLAB 500 s fluid path consists of PTFE based materials and borosilicate glass These materials are resistant to a wide variety of acids and bases at room to moderate temperatures Most chemicals do not affect PTFE at normal operating temperatures A Important Do not use the MICROLAB 500 for prolonged periods at high temperatures with aromatic or highly halogenated compounds These compounds may cause the MICROLAB s fittings to swell adversely affecting performance and reducing the life of system components Appendix D Chemical Compatibility of the MICROLAB 500
22. of calibrations traceable to N I S T HAMILTON THE MEASURE OF EXCELLENCE P O Box 10030 RENO NEVADA 89520 775 858 3000 B 2 MICROLAB 501A 503A and 504A User s Manual Appendix C Ordering Parts and Accessories for the MICROLAB 500 Appendix C contains information about ordering parts and accessories for MICROLAB 500 systems Table C 1 Reagent Diluent Syringe Replacement Parts Syringe Part Description Barrel Plunger Plunger Plunger Plunger Size Tip Assy Stop Tip w o Stop O ring 25 uL 80222 1702TLLX 10280 N A 13269 N A N A Syringe 50 uL 80922 1705TLLX 10222 N A 1117 01 N A N A Syringe 100 uL 81022 1710TLLX 10223 N A 1117 02 N A N A Syringe 250 uL 81122 1725TLLX 10339 N A 1117 03 N A N A Syringe 500 uL 81222 1750TLLX 10340 N A 1120 01 N A N A Syringe 1 0 mL 81323 1001TLLX 10341 N A 1578 01 N A N A Syringe 2 5 mL 81420 1002TLL 10227 N A 1360 01 N A N A Syringe 5 0 mL 81520 1005TLL 10203 13430 13230 N A 16114 Syringe 10 0 mL 81620 1010TLL 10204 13431 13231 N A 16104 Syringe 25 0 mL 82521 1025TLL 10448 N A 13271 N A N A Syringe Table C 2 Sample Syringe Replacement Parts Syringe Part Desc Barrel Plunger Plunger Plunger Plunger Plunger Size Tip Assy Stop Assembly Tip w o O ring Stop 25 uL 80226 1702DX 10436 N A 13269 N A N A N A Syringe 50 uL
23. of the volume of the installed syringe if you do not enter a specific volume Drive arm Speed defaults to various values based on the size of the installed syringe See Table 3 1 Default Syringe Speed Values for a list of the default speed values Chapter 3 Using the MICROLAB 500 System 3 5 Table 3 1 Default Syringe Speed Values Syringe Syringe Speed Size seconds per stroke 25 uL 2 50 uL 100 uL 250 uL 500 uL 1mL 2 5 mL 5mL 10 mL 25 mL CDN UTP Wl wl Nnini nun Using the Control Keys Use the control keys to enter specific information about your application such as the sizes of the installed syringes the volumes to be pipetted the drive arm speeds and the desired operation You can change the value of any control key or you can switch operations after the current operation completes 3 6 MICROLAB 501A 503A and 504A User s Manual Setting Syringe Sizes Note You must enter a size for the syringe s that are installed on the drive unit If you are using a dual syringe instrument you must enter a size for at least one of the syringes If you do not enter a size for the second syringe it remains inactive To enter a syringe size follow these steps 1 Press the left or the right Syringe Size key The left side Syringe Size key is active by default when you power on the MICROLAB 500 When you press a Syringe Size key the Control Indicator a
24. on the cord and insert it into the connector receptacle Then release the plastic locking device firmly attaching the cord Figure 2 2 Installing Electrical Connections I Accessory Hand Probe _ Holder Connector m Receptacle mMmicRO LAB 500 ee Parr Language Selection Switch CONTROLLER Controller Unit Connector Receptacle Gea Fuse Box oe Power Cord IgH Connector _ Receptacle Chapter 2 Installing the MICROLAB 500 System 2 5 Installing Valve Assemblies The MICROLAB 501A 503A and 504A all use different valve assemblies Instructions for installing valve assemblies follow Installing the Valve Assembly on the MICROLAB 501A The MICROLAB 501A uses a single active valve assembly To install the valve assembly follow these steps 1 Pick up the valve assembly Make sure the CTFE luer fitting that attaches to the syringe is on the bottom of the assembly 2 Holding the valve assembly align the valve stem opening with the valve motor drive port on the drive unit Press the valve assembly into place 3 Push down on the valve lever to lock the valve assembly to the drive unit Figure 2 3 Installing a Valve Assembly on the MICROLAB 501A Valve Stem Valve CTFE Luer Fitting Valve Motor __ _ Drive Port Valve Lever Lock 2 6 MICROLAB 501A 503A
25. position to stop the priming cycle Press the Step Prime switch to the Step position and advance the syringe drive arm to the Home position The system is now clean and ready for use Note When priming reagent after a deionized water rinse dispense the first shot into a waste container since it will contain a certain amount of the water droplets that remained in the tubing line Cleaning Syringes and Tubing A Warning Follow your laboratory s safety procedures if you use the MICROLAB 500 system to handle hazardous materials A When tubing and syringes come in contact with contaminated or hazardous samples follow safe laboratory practices in selecting and using a cleaning fluid to flush out the tubing and syringes Purge clean and decontaminate the MICROLAB 500 by thoroughly flushing out all portions of the tubing and syringes Chapter 4 Caring for the MICROLAB 500 4 3 Use a cleaner that is compatible with the fluids previously run through the system Depending on the sample that is being run you may want to use de ionized water urea ethanol or a 10 chlorine bleach and deionized water solution as a cleaning fluid Clean syringe plunger tips by soaking them in Hamilton Syringe Cleaning Solution If you still have questions contact Hamilton Company s Technical Customer Service Department at 800 648 5950 in the United States and Canada Cleaning the Exterior of the MICROLAB 500 A Warning Follow your
26. provides a list of chemicals and their compatibility with the MICROLAB 500 system A If you have any questions about chemical resistance to specific compounds please contact the Hamilton Company check out any unusual or reactive compounds before using them with your MICROLAB 500 system Storing the MICROLAB 500 For long term storage prime and purge the system with methanol to facilitate drying Remove the tubes and syringes Cover the instrument to protect it from damage Store syringes in their original containers Chapter 4 Caring for the MICROLAB 500 4 5 Chapter 5 Troubleshooting the MICROLAB 500 This chapter contains information about the following topics e message codes e troubleshooting e technical support e returning instruments for repair Please read this chapter some problems may be easily resolved by using the information found in the message code and troubleshooting guides Message Code Guide The MICROLAB s display windows provide you with system status and with error message information If an error occurs an error message appears the system beeps three times and all operation and function keys are disabled To recover from the error reinitialize the system by pressing the Run Stop key You cannot use the probe button on a hand probe to reinitialize the system Table 5 1 lists the messages that may appear in the message display windows Table 5 1 Message Code Guide Left Side Dis
27. sub assemblies and together they create a very versatile and functional instrument Figure 1 5 shows the front view of the MICROLAB 501A single syringe dispenser In this figure the controller unit rests on top of the drive unit and a syringe is attached to the syringe drive arm A Warning This warning label appears on the front panel of the ML500 It indicates that a pinch hazard exists when the syringe drive is moving A 1 8 MICROLAB 501A 503A and 504A User s Manual Figure 1 5 Front View of the MICROLAB 501A Single Syringe Dispenser 2s 10m 50 100 EN 25ul C Aa Game Cum L amp L C Ter e C CO oe Hand Probe Connector Receptacle tle Step Prime Z6 Switch J Power Indicator Light SEn o Power A On Off W Switch Chapter 1 Getting Started 1 9 Figure 1 6 shows the front view of the MICROLAB 503A dual syringe diluter In this figure the controller unit rests on top of the drive unit and syringes are attached to the syringe drive arms Figure 1 6 Front View of the MICROLAB 503A Dual Syringe Diluter
28. the return of any instrument or valve that has been used with radioactive or microbiological substances or any other material that may be deemed hazardous to employees of Hamilton Company July 1999 by Hamilton Company GASTIGHT is a trademark of Hamilton Company MICROLAB is a trademark of Hamilton Company SANI CLOTH is a trademark of Professional Disposables Inc Instruction to the User This equipment has been tested and found to comply with the limits for a class B digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference in an installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different from that to which the receiver is connected e Consult the dealer or an experienced radio TV technician for help
29. AB 500 system Information in this chapter includes e MICROLAB 500 parts lists e MICROLAB 500 components the drive unit the controller unit hand pipettors probes All MICROLAB 500 instruments come with everything you need to start using the system with the exception of syringes You must separately purchase syringes for use with the MICROLAB 500 systems For complete lists of syringes accessories and replacement parts for the MICROLAB 500 see Appendix C Ordering Parts and Accessories for the MICROLAB 500 Note Contact your local delivery company if you notice any visual damage to the MICROLAB 500 shipping package or to its contents Also you may want to save the shipping container in case you ever need to return the instrument for service MICROLAB 500 Parts Lists This section includes complete parts lists for the MICROLAB 501A 503A and 504A systems After you unpack your MICROLAB 500 check to see that you have received all parts before attempting to set up the system The parts lists are presented in four separate tables with four corresponding figures Table 1 1 lists the non programmable models in the MICROLAB 500 series their components and each component s part number Figure 1 1 shows these components Table 1 1 MICROLAB 500 System Descriptions Model Part Drive Controller Manual Part Power Shipping Unit Unit amp Cord Kit Part Cord Part
30. Clean the MICROLAB 500 ceeeeeee 4 2 Cleaning the Fluid Path of the MICROLAB 500 ceeee 4 2 Cleaning Syringes and Tubing 0 ccceeeeeeeeeeeeeeeeeeeaaeeeeeeeeees 4 3 Cleaning the Exterior of the MICROLAB 500 ccceeesseeees 4 4 Chemical Compatibility 0 cceccecceeeeeeeeeeeeeeeaaeaeeeeeeeeeeeeaeaaee 4 5 Storing the MICROLAB 500 ccc eeeeeeeeeeeaeeeeeeeeeeeeeeaaeeeeeeeees 4 5 Chapter 5 Troubleshooting the MICROLAB 500 5 1 Message Code Guide ccceceeeeeeeceeeeeeeeeeeeeeeeeaaeaeeeeeeeeeeaeaaes 5 2 Audible Messages cccecceeeeee ee eeeeeeeeeeeeeeseeeeeeseaeeeeensaneees 5 4 Troubleshooting Guide cceccceeeeeeeeeeeeeeeaeeaeeeeeeeeeeeeaeaaaaeeeees 5 5 Getting Technical SUDPOMt ce cece ce eeeeeee ee eeeeeeeeeeeeaeaaeaeeeeeeees 5 9 Returning Instruments for Repair eeceeeeeeeeeeeeeeaeeeeeeeaeeeeees 5 10 Appendixes Appendix A Technical Specifications for the MICROLAB 500 068 A 1 Appendix B Instrument Performance Test Reports cccccsseeeeeesteeeees B 1 Appendix C Ordering Parts and Accessories for the MICROLAB SOO eea e ete nea eae eta tie C 1 Appendix D Chemical Compatibility of the MICROLAB 500 ceee D 1 Glossary ordenta miana aa nae Eai iaaa A aaa GL 1 mador Reece eee cre T cic re tae cise oh ee cee a reiterates IN 1 viii Figures and
31. MICROLAB 500 system 1 Condition all syringes before installation by following the procedure described in Preparing Syringes for Installation 2 Power on the MICROLAB system using the Power On Off switch 3 Press and hold the Step Prime switch in the Step position and move the syringe drive arm down from the Home position Release the switch when the drive arm is about half way between Home and the Down position 2 10 MICROLAB 501A 503A and 504A User s Manual 4 Fasten the thumbscrew on the drive arm to the bottom of the syringe plunger To fasten the thumbscrew hold the plunger and screw the thumbscrew into the threaded fitting at the bottom of the syringe 5 Pull the glass barrel up straight to the threaded female luer fitting that extends down from the bottom of the valve Insert the male luer fitting into the valve fitting and turn the glass barrel clockwise until it is finger tight See Figure 2 8 Installing a Syringe for an illustration of these steps Figure 2 8 Installing a Syringe Q Then mount the Luer Lock First mount the Thumbscrew Chapter 2 Installing the MICROLAB 500 System 2 11 A Important Always tighten syringes so they are finger tight Syringes that are over or under tightened can cause problems for your MICROLAB 500 system e Syringes that are over tightened may cause leaks or may damage the valve e Syringes that ar
32. The MICROLAB 500 Series MICROLAB 501A MICROLAB 503A and MICROLAB 504A HAMILTON THE MEASURE OF EXCELLENCE Part Number 69175 Rev E Hamilton Company Instrument Warranty Hamilton Company warrants this equipment except valves to be free of defects in material and workmanship for 12 months from the date of receipt This warranty is extended to the buyer of record on the original purchase order to Hamilton Company Hamilton Company or an authorized Hamilton representative agrees to repair or replace at its option and free of charge to the buyer at a normal place of business or at a Hamilton repair facility any part or parts that under proper and normal use prove to be defective during the warranty period Abuse unauthorized replacement of parts modifications or adjustments made by other than Company or its assigned representatives voids this warranty This warranty gives you specific rights No other warranties expressed or implied including implications of warranties of merchantability and fitness for a particular product are made Hamilton Company s liability on the sale of all products shall be limited to repair replacement or refund of price of any defective product Hamilton Company endeavors to provide prompt and satisfactory service All Hamilton Company valves are warranted to be free of defects in material and workmanship at the time of delivery Hamilton Company reserves the right to refuse to accept
33. age appears only on dual syringe units The left valve is stalled Over Temp The unit is overheated Power off the unit and allow it to cool down Check for the reason is ventilation adequate Is the room temperature appropriate See Appendix A Technical Specifications for information about appropriate environmental conditions Resume use at a lower duty cycle continued Chapter 5 Troubleshooting 5 3 Table 5 1 Message Code Guide continued Left Side Right Side Meaning Action Display Display Priming Priming The unit is in prime or step mode This message is displayed until the mode is stopped Exceeds Volume The specified volume exceeds the capacity of the syringe Enter a new volume that is within the capacity of the syringe Audible Messages When a MICROLAB 500 system is operated via the controller unit the system will provide audible messages beeps that indicate certain events These events are listed in Table 5 2 Table 5 2 Audible System Messages Number Event of Beeps 2 The system beeps twice upon power up at the end of power up diagnostics 1 The system beeps once at the start of an operation 3 The system beeps three times if an error occurs 5 4 MICROLAB 501A 503A and 504A User s Manual Troubleshooting Guide Table 5 3 provides quick answers to help you troubleshoot your MICROLAB 500 Table 5 3 Troubleshooting Guid
34. an or equal to 10 mL and with viscous or volatile fluids Syringe sizes of 250 uL to 5 mL may be run at speed 1 Hamilton Company reserves the right to alter technical specifications without notice Table A 2 contains specifications for syringe accuracy and precision This information is on file at Hamilton Company in Reno Nevada Table A 2 Accuracy and Precision 1 mL of Deionized Water at 23 C Percent Accuracy Precision Stroke 1 3 1 5 5 1 2 5 30 1 0 2 Best reproducibility and accuracy are achieved at syringe stroke lengths equal to or greater than 10 of syringe volume using Hamilton syringes Liquid handling performance may vary according to viscosity temperature and technique A 2 MICROLAB 501 503 and 504 Operator s Manual Appendix B Instrument Performance Test Reports All instruments are subjected to quality control performance testing before they leave Hamilton Company Each MICROLAB 500 system is shipped with a copy of its own performance test report The report contains the actual results of a gravimetric test conducted on the instrument by Hamilton Company s Quality Control Department Keep your MICROLAB 500 s performance test report for use as a benchmark of its original precision and accuracy You may wish to periodically test your MICROLAB 500 and compare its current performance with its out of box performance Remember when you test an instrument you
35. and 504A User s Manual Installing Valve Assemblies on the MICROLAB 503A and 504A Although the valve assemblies for the 503A and 504A units are different from one another their installation procedures are the same To install a valve assembly on either the MICROLAB 503A or 504A follow these steps 1 Pick up the valve assembly Make sure the CTFE luer fittings that attach to the syringes are on the bottom of the assembly 2 Holding the valve assembly align the valve stem openings with the valve motor drive ports on the drive unit If they do not align power on and initialize the drive unit The valve stem openings and the drive ports align when the unit is initialized 3 Insert the valve assembly into the valve motor drive ports and press the valve assembly into place 4 Tighten the thumbscrew until it is finger tight to secure the valve assembly to the drive unit Figure 2 4 Installing a Valve Assembly MICROLAB 503A and 504A Valve Motor Drive Ports SN _ sy J Valve Stem Valve Thumbscrew Chapter 2 Installing the MICROLAB 500 System 2 7 Selecting Installing and Removing Syringes Before you install syringes on any MICROLAB instrument you must first decide what syringes to use Use Tables 2 2 and 2 3 to select the best syringe for your application See Figures 2 5 through 2 7 for examples of different syringe types e The MICROLAB 501A uses one dispenser diluent type
36. aning the fluid path If you still have questions contact Hamilton Company s Technical Customer Service Department at 800 648 5950 Cleaning the Fluid Path of the MICROLAB 500 Clean the MICROLAB 500 s fluid path before using it for the first time Use Hamilton Syringe Cleaning Solution to clean the fluid path See Appendix C for information on ordering Hamilton Syringe Cleaner To clean the fluid path follow these steps 1 Prepare a reservoir of cleaning fluid and place the fill tubing in the cleaning fluid reservoir Use a tubing clip to secure the tubing to the side of the reservoir 2 Place the hand probe into the cleaning fluid reservoir or a waste container 4 2 MICROLAB 501A 503A and 504A User s Manual 10 11 Press the Step Prime switch on the drive unit to start the priming cycle Continue the priming cycle until the fluid path is clean The number of cycles needed to clean the fluid path will depend on the sizes of the syringes and the dirtiness of the system When the fluid path is clean press the Step Prime switch back to the middle position to stop the priming cycle Move the inlet tubing from the cleaning fluid reservoir and place it into a reservoir of deionized water Press the Step Prime switch to continue the priming cycle Continue the priming cycle until the fluid path is free of all cleaning fluid Dispense the rinse fluid into a waster reservoir Press the Step Prime switch to the inactive
37. apter 2 for installation instructions Syringe Drive Arms You attach syringes to the MICROLAB s syringe drive arms The syringe drive arms are engineered to drive Hamilton precision syringes with high resolution stepper motors the motors and drive arms are connected by a precision lead screw See Selecting Installing and Removing Syringes in Chapter 2 for syringe installation instructions Chapter 1 Getting Started 1 15 The Controller Unit The controller unit is a portable device that connects to the syringe drive unit via the controller connector cord The controller unit sends display information and instructions to the drive unit Figure 1 9 shows the controller unit Two message display windows are located at the top of the unit and three main groups of controls are located across the center and the bottom of the unit The controls are divided into three functional groups e function control keys e operation keys Run Stop key Figure 1 9 The Controller Unit Left Side Message Right Side Message Right Syringe Size Indicator Right Side Syringe Size Selector Key Right Volume Control Key Right Speed Control Key Display Window Display Window Left Syringe Size Indicator Increase Key 25 25 N a _ 25 Ji_J 250 250 p 25ul 25ul Left Side Syringe Size Selector Key Control Key ZN Left Spe
38. care to keep the instrument clean Follow all instructions for using and handling the system Figure 3 1 Using the MICROLAB 500 Press the Prime Switch and start priming the instrument 1 Clean the fluid path No Check the fluid path for leaks Adjust tubings fittings and syringes Any leaks in the fluid path Yes Any bubbles on the plunger tip Clean the path Halt the priming cycle 4 Use the control keys Set syringe size s e Set syringe volume s e Set syringe drive speed s j Select an operation mode Press the Up key OR e Press the Down key OR Press the Auto Refill key Press the Run Stop key or the probe button to start the operation Finished Select using the another instrument operation When finished flush the fluid line and turn the instrument off 3 2 MICROLAB 501A 503A and 504A User s Manual Using the System An Overview Before using your MICROLAB 500 system be sure you have followed the installation instructions in Chapter 2 Installing the MICROLAB 500 Figure 3 1 provides an overview of using the system Powering On the MICROLAB 500 To power on the MICROLAB 500 press the Power On Off switch located on the front panel of the drive unit When the instrument powers up it performs an electronic self test beeps twice and disp
39. cator lights on the MICROLAB 500 luer fitting The standard twist and lock type of fitting message display windows The two areas on the controller unit where system messages and information appear GL 4 MICROLAB 501A 503A and 504A User s Manual MICROLAB 501A Hamilton Company s single syringe dispenser MICROLAB 503A Hamilton Company s dual syringe diluter dispenser MICROLAB 504A Hamilton Company s dual syringe dispenser operation The Up Down Auto Refill or Prime functions performed by the MICROLAB 500 systems operation indicator lights LED lights that show the current functions being performed by a MICROLAB 500 system operation keys The Up Down and Auto Refill images on a MICROLAB 500 controller unit Each image represents a function press on the image to activate the function overload A stall in syringe plunger movement that may be caused by excess mechanical friction excess syringe speed or a blockage in the valves tubing or syringes pipette To measure a fixed amount of liquid with a probe power down To turn off an electrical device Power Indicator light A LED on the drive unit that shows whether or not the MICROLAB system is powered on Power On Off switch A switch used to turn the MICROLAB system on power on To turn on an electrical device Glossary GL 5 prime To make an instrument ready for operation by running fluid through the tubing lines of an instrument to en
40. contains the technical specifications for MICROLAB 500 Table A 1 Technical Specifications for the MICROLAB 500 Reagent Diluent Syringe 25 uL to 25 mL TLL or TLLX type Reagent Diluent Volume Range 1 uL 25 000 uL Sample Syringe 25 uL to 1 mL DX type Sample Volume Range 1 uL 1 000 uL Accuracy and Precision See Table A 2 Resolution 1 of syringe drive Volume Increments 1 of total syringe volume adjustable Speed 2 to 20 seconds per full syringe stroke adjustable Drive High precision lead screws driven by a highly efficient stepper motor Power Requirements 115 220 V 50 60 Hz power supply automatically converts Power Rating 150 VA Weight 10 lbs 6 oz 4 7 kg Dimensions Height 13 1 8 in 33 34 cm Width 6 1 8 in 15 56 cm Depth 7 in 17 78 cm continued Table A 1 Technical Specifications continued Shipping Dimensions Height 14 1 4 in Width 21 in Depth 141 2 in Shipping Weight 20 lbs Storage Temperature Range 20 to 50 C 4 to 122 F Operating Temperature 5 to 40 C 41 to 104 F Range Recommended Operating 18 to 25 C 64 to 77 F Temperature Range Humidity Range 10 90 non condensing Fluid Path PTFE based materials and Borosilicate glass Use slower drive speeds and 12 gauge tubing with larger syringes larger volumes greater th
41. ction switch is located on the back of the drive unit below the serial number label see Figure 2 2 To change the displayed language power the instrument off Then use a screwdriver to change the switch position The small bump on the switch indicates the selected position By changing the position of the Language Selection switch you can view message codes in five different languages including English French German Portuguese and Spanish Switch positions for each language are shown in Table 2 1 Table 2 1 Language Selection Switch Positions Switch Position Language 0 English 1 French 2 German 3 Portuguese 4 Spanish Installing Electrical Connections Refer to Figure 2 2 and follow these steps to install electrical connections 1 Plug the power cord into the power connector receptacle on the back of the drive unit The power cord fits into the receptacle only one way The power connector receptacle accepts cords for either 115V or 220V 2 Plug the hand probe or foot pedal jack into the probe connector receptacle The probe connector receptacle is located on the upper right side of the drive unit 2 4 MICROLAB 501A 503A and 504A User s Manual 3 The controller connector receptacle is located on the back of the drive unit below the serial number plate Plug the controller cord into the receptacle just as you plug a telephone cord into a telephone outlet Pinch the plastic locking device
42. description of 8 syringes cleaning 4 3 installing 2 10 to 2 12 precautions about removing 2 13 preparing for installation 2 10 removing 2 12 to 2 13 selecting 2 8 to 2 9 system descriptions PR 3 overview of 3 3 powering on 3 3 speed default values 3 5 T technical support telephone numbers for 5 9 troubleshooting guide 5 5 tubing cleaning 4 3 difference between fill and dispense 2 14 15 installing 2 15 to 2 19 selecting 2 14 to 2 15 U Up key description of 1 20 upgrading the MICROLAB 500 system PR 3 V valve assembly 1 15 valve assembly installing 2 6 to 2 7 volume keys description of 1 18 Ww writing conventions PR 5 Index IN 3
43. e Problem Possible Causes Corrective Action instrument does not e disconnected e connect power cord and check power up power cord or power source faulty power outlet e main fuse is e check main fuse on instrument s blown rear panel replace fuse if necessary instrument e faulty LED or e check to see that the controller functions normally but indicators or message display windows fail to illuminate LCD unit is plugged into the drive unit call the Hamilton Service Department keys are non functional instrument is in pause mode e ifUnit Paused appears in the display windows press the Run Stop key to finish the stroke valve or syringe is stalled e internal fault if a Stall message appears in the display windows reinitialize the system by pressing the Run Stop switch if message persists call the Hamilton Service Department continued Chapter 5 Troubleshooting 5 5 Table 5 3 Troubleshooting Guide continued Problem Possible Causes Corrective Action instrument does not e faulty or blocked check tubing valve and fill or dispense tubing blocked or syringes for blockages crimps leaky fluid path or loose fittings replace tubing either replace or reseal valve and syringes tighten valve and syringes e incorrect tubing or check that appropriate tubing syringe fittings and syringes are used connections and that they are finger t
44. e arms to which syringes connect The movement of the syringe drive arms displaces fluid drawing it through the system Syringe Size key A key located on the controller unit used to select the syringe size function system fluid Refers to the fluid originating from a reservoir pumped through the entire system tubing clip A device that attaches to the side of the reservoir and holds tubing lines in place Up The function that moves the syringe drive arms to the Home position valve The device that directs the flow of liquid through the system valve port Openings in the valve assembly through which liquid flows As the valve turns ports are opened or closed directing the flow of liquid through the system Volume key A key located on the controller unit used to select the syringe volume function Glossary GL 7 Index A Auto Refill key description of 1 21 Cc checking the system for leaks 3 4 cleaning See Instruments cleaning Concorde Push button Hand Pipettor Probe 1 22 controller unit connector receptacle 1 14 2 5 description of 1 16 D default values for drive speeds 3 6 Down key description of 1 20 F footswitch description of 1 24 function control keys 1 17 fuse box 1 13 H hand probe connector receptacle 1 13 2 4 l increase key 1 17 installing accessory holder 2 3 electrical connections 2 4 syringes 2 10 to 2 12 tubing 2 15 valve assemblies 2 6 instruments
45. e no bubbles or air gaps in the tubing Check the fluid path for leaks and clean the fluid path if necessary To prime the MICROLAB and check the fluid path for leaks follow these steps 1 Fill a reservoir with deionized water Place the end of the inlet tubing in the reservoir Use the tubing clip to attach the tubing to the side of the reservoir Place the hand probe over the same reservoir or over a waste container Push the Prime switch on the drive unit Run the instrument in Prime mode until water flows through the entire system SIF Ss car ah If the system is airtight the fluid path will contain a solid column of water If you see air bubbles in the fluid path the system is not airtight Adjust the tubing fittings or syringes Check that all parts are snug and finger tight If you do not see air bubbles in the fluid path the system is airtight 6 Ifyou do not see air bubbles in the fluid path but small bubbles appear on the PTFE surface of the syringe clean the internal fluid path with a mild syringe cleaner Empty out the soapy water and rinse with deionized water to rid the system of cleaning fluid To clean the fluid path follow the steps in Chapter 4 Caring for the MICROLAB 500 7 After cleaning reprime with deionized water to rinse the fluid path Continue priming until you do not see any bubbles 8 Remove the inlet tubing from the reservoir and prime air through the system Priming air through the sy
46. e reverses and connects the fluid path from both syringes to the hand probe When the probe button is pressed a second time the syringe drive arms move up Sample and diluent are dispensed and mixed together creating a dilution Figure 3 3 shows the Disposable Tip Probe being used for dilutions Figure 3 3 Dilutions Dispense Aspirate Diluted Sample Sample 3 12 MICROLAB 501A 503A and 504A User s Manual Example 2 Dilutions MICROLAB 503A Example 2 follows the same procedures as those shown in the MICROLAB 503A Example 1 except that two TLL syringes are used and the dispense tubing is connected directly to the side of the valve assembly To attach tubing directly to the valve assembly remove the plug on the side of the valve Replace the plug with tubing Figure 3 4 shows the Concorde Hand Probe being used for serial dilutions Figure 3 4 Serial Dilutions iN Ss lt Diluent Serial Dilutions Chapter 3 Using the MICROLAB 500 System 3 13 Sample MICROLAB 504A Application Configurations Example 1 Dual Dispensing When the probe button is pressed once the syringe drive arms move down pulling in two different liquids from two different reservoirs The valves then reverse and connect the fluid path from the syringes to the hand probe When the probe button is pressed a second time the syringe drive arms mo
47. e under tightened may cause leaks e Syringes that are not screwed on straight may leak and cause lateral strain on the syringe luer fitting as it connects to the bottom of the valve A Removing Syringes To remove syringes reverse the installation procedure 1 Power on the system using the Power On Off switch 2 Use the Step Prime switch to move the syringe drive arm down from the Home position Release the switch when the drive arm is about half way between Home and the Down position Release the glass barrel by turning it counterclockwise 4 Unfasten the thumbscrew on the drive arm at the bottom of the syringe plunger To unfasten the thumbscrew hold the plunger and unscrew the thumbscrew from the threaded fitting at the bottom of the syringe Refer to Figure 2 9 Removing a Syringe for an illustration of the removal procedure 2 12 MICROLAB 501A 503A and 504A User s Manual Figure 2 9 Removing a Syringe First remove the Luer Lock Then remove the Thumbscrew AN Warning Avoid the risk of injury or infection Use extreme caution when removing cracked or splintered syringes Always wear thick gloves and protective eye wear when replacing syringes A Chapter 2 Installing the MICROLAB 500 System 2 13 Selecting and Installing Tubing This section provides information about selecting and installing tubing on all MICROLAB 500 systems
48. ear view of all units Figure 1 8 Rear View of the MICROLAB 501A 503A and 504A fj mano eee Connector Receptacle E m Es EE m p MAINA LAB 500 scnies ae a E o Language Selection Switch Controller Unit Connector Receptacle L eS Fuse Box 2L Power Cord O Connector Receptacle 1 12 MICROLAB 501A 503A and 504A User s Manual Power Cord Connector Receptacle The power cord connector receptacle is located on the back of the drive unit the power cord fits into this receptacle only one way The receptacle accepts cords for either 115V or 220V without any adaptation of the drive unit See Figure 1 4 for the location of the power cord connector receptacle Hand Probe or Footswitch Connector Receptacle The hand probe connector receptacle is located on the upper right side of the drive unit You can insert either a hand probe jack or a footswitch jack into this receptacle See Figures 1 1 through 1 3 for the location of the hand probe connector receptacle Fuse Box The fuse box is located at the top of the power cord connector receptacle To gain access to the fuse box you must first remove the power cord See Figure 1 4 for the location of the fuse box Language Selection Switch The Language Selection switch is located on the back of the drive unit below the serial number label See Figure 1 4 for the location of this s
49. ed O Votume Ie z i Control Key ay ofe aat V ma ee Operation Mode Indicator Lights s Run TILLA a Up Down Auto Refill HAMETON Run Stop Key Operation Mode Keys Decrease Key 1 16 MICROLAB 501A 503A and 504A User s Manual For ease of use and for everyday storage the controller unit is designed to fit on top of the drive unit Message Display Windows The message display windows located at the top of the controller unit show the status of the instrument and provide information about current control settings e the size of the left or the left and right syringes e the selected volume for the left or the left and right syringes e the drive speed for the left or the left and right syringes e the volumes being pipetted when the MICROLAB is in operation e all system information or error messages See Figure 1 9 for the location of the display windows Function Control Keys The keys by which you send information to the drive unit are marked with images or icons that represent each key s function Use the keys to enter information about the syringes volumes speeds and operations Increase and Decrease Keys To increase syringe size pipetting volume or syringe drive A speed press the Increase key Increase To decrease syringe size pipetting volume or syringe drive V speed press the Decrease key Decrease After you select a specific function key and t
50. ee 1 24 Probe Button Functions cceeccceeeeeeceeeeeeeaaeeeeeaaeeeeeeaeeees 1 24 Installing the MICROLAB 500 System 55 2 1 Overview of Installation Procedures cc cccceeeeeeeeeeeeaeeteeeeeees 2 3 Selecting a Location ccceceeeee cece eeeeeeeeeeeeeeeeaaaaeeneeeeeeeeeaeaaee 2 3 Installing the Accessory Holder ccccceeeeeeceeeeeeeeeeeeeeeeeeeeaeees 2 3 Selecting a LANQUAGC eccceeeee eee eeeeeeeeeeeeeeaeaaaeeeeeeeeeeeaeanes 2 4 Installing Electrical CONNECTIONS cceeeeeeeeeeeee eee tteeeeeeeeeeeaes 2 4 Installing Valve ASSEMDIiICS eee cece ee eeee eee eeeeeeeeeeeeeeeeeeeeeeaaae es 2 6 Installing the Valve Assembly on the MICROLAB 501A 2 6 Chapter 3 Installing Valve Assemblies on the MICROLAB 503A and 504A ce cecceeeceeeeeeee cece eeeeeeeeeeeaeaaeeees 2 7 Selecting Installing and Removing Syringes ceeseeeeees 2 8 Preparing Syringes for Installation eeaeee 2 10 INStalliING SYrINGES cs s cisseieehacoadastidebevtacooebaasidevanedperecartaeets 2 10 REMOVING Syringes cce cece eeeeeeeeeeeeeeeeaeaaeeeeeeeeeeeeaeaaeaeenees 2 12 Selecting and Installing TUDING 0 eceeeeeeeeeeeeeeeeeeeaeeaee ees 2 14 Selecting TUDING ccccceceeeeeeeeeeeeeeeeeeeeeeeseaaaaeeeeeeeeeeaeaaea 2 14 INStalling TUDING retroterra etere r rE OED 2 15 Using the MICROLAB 500 System c eeeeeeee 3 1 Using the Syst
51. election switch i Plug in electrical connections power cord e hand probe controller unit cord y Install the valve assembly Install syringe s Install the fill tubing y Install the tubing clip Y Install the dispense tubing 1 Run the dispense tubing through the hand probe 4 Fill out and return warranty card Installation w MICROLAB 501A 503A and 504A User s Manual Overview of Installation Procedures Figure 2 1 provides an overview of the MICROLAB 500 installation procedures These procedures are described in detail in this chapter Selecting a Location Install your MICROLAB 500 system in a clean dry level area away from hazardous fumes Leave space around the unit for ventilation three inches is sufficient Installing the Accessory Holder All MICROLAB systems come with an accessory holder You can mount the accessory holder on either the right or the left side of the instrument Generally the accessory holder is mounted on the right side and is used to hold a hand probe when the probe is not in use To install the accessory holder follow these steps 1 Locate the threaded holes on either the right or the left side of the instrument 2 Use a hex wrench and screws screws are provided to attach the holder Chapter 2 Installing the MICROLAB 500 System 2 3 Selecting a Language The Language Sele
52. em An Overview ccceceeeeeeeeeeeeeeeeeeeaeaaeeees 3 3 Powering on the MICROLAB 500 ceceeeeeeeeee esac eeeeeeeeeeeeeaes 3 3 Priming and Checking ccceeeeeeeeeeeeeeeaaeeeeeeeeeeeeeeaeaaeeeeereeeees 3 4 Default Valles i w neice ee vee nae ee 3 5 Using the Control Keys ececeeeeeeeeeeeeeeeeeeeeeaeaaeeteeeeeeeeeaeaaes 3 6 Setting Syringe Sizes 0ceceeeee cece eeeeeeeeeeeeee ee ae aaeeeeeeeeeeeeaeaaea 3 7 Setting Syringe VOIUMES cceeeeeeeee eee eeeeeeeeeeeeeeeaaeaeeeeeeees 3 8 Setting Syringe Drive Speeds 0 ccceeeeeeeeeeeeeeeeeeeeaeeeeeeeeees 3 9 Running Operations re aaa a naan anaana ia i eaa Aa AEN 3 9 Pausing Operations ccccccceeeeeeeeeeeeeeeeeeeeeeeeeaeaaeeeeeeeees 3 10 Sample MICROLAB 501A Application Configurations 3 11 Single Syringe Dispensing c ceeeeeeeeeteeeeeeeeeeeeaee 3 11 Sample MICROLAB 503A Application Configurations 3 12 Example 1 Dilutions cece ee eeneeeeeeeeeeee ena eeeeeeaaeeeeeaa 3 12 Example 2 Dilutions asr ekan EE REER 3 13 Sample MICROLAB 504A Application Configurations 3 14 Example 1 Dual Dispensing eceeeeeeeteeeeeeeeeeeees 3 14 vii Example 2 Using the Dual Dispenser for Single Dispensing ccceeeeeeeeeeeeeeeeeeeaaeneeeeeeeeeaeaaed 3 14 Chapter 4 Caring for the MICROLAB 500 cseeeeeeees 4 1 Deciding When to
53. g lines aspirate To pick up a designated volume of sample or air through a pipette or a probe tip Auto Refill In this operation the drive arm moves down after fluid is dispensed to automatically refill syringes cavitation An occurrence caused by applying a high vacuum to a liquid gas dissolved in liquid can be pulled out of solution It generally occurs when large syringes 5 and 10 mL are driven at high speeds Concorde hand probe The Concorde hand probe is Hamilton Company s standard single hand activated probe The tip of this probe is adjustable and allows the user to set the dispense tubing to an angle that is comfortable for use control indicators LEDs that show the active function on the controller unit controller unit The device that allows the user to send operating instructions to the drive unit cycle A series of two strokes that comprise both the up and down movements of the syringe drive arm Decrease key A control used to decrease the settings for syringe sizes volumes or speeds default A predetermined value in a program or in computer circuitry that an operator may or may not alter diluent A liquid that is added to a sample to lessen the sample s concentration i e to dilute the sample diluent syringe A syringe that delivers a diluent On a dual syringe unit the diluent syringe is usually the syringe on the left dilute To reduce the concentration of solutes in a sample by adding a
54. g syringe install dispense tubing directly onto the valve assembly To do this remove the valve plug and replace the plug with dispense tubing See Figure 2 12 2 16 MICROLAB 501A 503A and 504A User s Manual Figure 2 11 MICROLAB 503A Valve with a D Configuration Syringe Insert Valve Plug First Figure 2 12 MICROLAB 503A Valve with Two TLLX or TLL Syringes Remove Valve Plug First Chapter 2 Installing the MICROLAB 500 System 2 17 If you are using a MICROLAB 504A install a tapered dispense tubing line into each of the valve s front ports The front ports are located on the bottom of the valve between the two syringes Thread the tubing fittings into the front ports and screw the fittings in so they are finger tight See Figure 2 13 Figure 2 13 MICROLAB 504A Valve and Tubing Connectors A Important There are two methods of using the MICROLAB 504A dual dispenser as a single dispenser Method 1 Install dispense tubing on the unused side of the instrument and pull in distilled water from a reservoir Cycle the distilled water through the unused side Dispense the water back into the same reservoir or into a waste container Do not run the unused side dry Method 2 Set the syringe size on the left or the right side syringe to 0 zero The side that is set to 0 remains idle when you operate the instrument A 2 18 MICROLAB 501A 503A and 504A User s Manual 6 Install the d
55. he fill tubing You can differentiate fill tubing and dispense tubing since dispense tubing has a tapered end For all MICROLAB 500 models install the fill tubing on the left side of the valve Thread the tubing fitting into the left valve port and screw it in so it is finger tight For the MICROLAB 504A only install a second fill tubing line on the right side of the valve Thread the tubing fitting into the right valve port and screw it in so it is finger tight just as you did on the right side 4 Once the fill tubing is installed attach a tubing clip to it Slip the tubing clip over the end of the tubing then attach the clip to the side of the reservoir Chapter 2 Installing the MICROLAB 500 System 2 15 5 Install the dispense tubing You can recognize dispense tubing by its tapered end Ifyou are using a MICROLAB 501A install the dispense tubing on the right side of the valve Thread the tubing fitting into the right valve port and screw it in so it is finger tight See Figure 2 10 for an illustration of the valve and tubing connectors Figure 2 10 MICROLAB 501A Valve and Tubing Connections S0 ee If you are using a MICROLAB 503A with a D syringe install the dispense tubing on the side port of the D syringe See Figure 2 11 When using a TLL syringe as a samplin
56. hen press the Increase or Decrease key the new size volume or speed displays in the message display windows Chapter 1 Getting Started 1 17 Syringe Size Keys The Syringe Size keys allow you to select either or both on a two syringe instrument the left or the right syringe Once a syringe is selected you can adjust its size volume or speed You can select either the left or the right syringe in any order the system defaults to the left syringe You must select a syringe size before you can change its volume or speed Press the left Syringe Size key to select the left syringe Then press the Increase or the Decrease key to change its size Press the right Syringe Size key to select the right syringe Then press the Increase or the Decrease key to change its size When you select either the left or the right Syringe Size key a green LED indicates which of the keys is active As you change the size of a syringe notice that the syringe size indicator lights located on either side of the message display windows move to reflect the size of the syringe Note The syringe size indicators show syringe sizes over 500 uL in milliliters but the message display windows show the syringe size in microliters Volume Keys To set the volumes you wish to pipette press either the left or the right Volume key and then press the Increase or the Decrease key Note You must enter a syringe size before you can change its volume
57. ight e faulty keys call the Hamilton Service Department e non functional call the Hamilton Service syringe drive s Department e valves do not e remove and reseat valve rotate valve tighten thumbscrew or replace drives are not valve engaged or valve is faulty continued 5 6 MICROLAB 501A 503A and 504A User s Manual Table 5 3 Troubleshooting Guide continued Problem Possible Causes Corrective Action instrument gives inaccurate or imprecise results e air exists in the fluid path e incorrect size of dispense tubing used with syringe e check to see that tubing ends are totally submerged in reagent bottles e check to see that tubing connections are tight replace if necessary e check to see that the syringe is installed correctly and that it is not leaking e replace worn or leaking valve or tubing e reduce the syringe drive speed to eliminate cavitation problems e check for correct tubing and fittings e use small tapered tubing 18 gauge for small dispense volumes small air gap appears at tip of probe tubing after final aspiration e dirty tubing e improper aspiration e change or clean the tubing e lower the aspiration speed continued Chapter 5 Troubleshooting 5 7 Table 5 3 Troubleshooting Guide continued Problem Possible Causes Corrective Action persistent air or leaks in the fluid path e ca
58. igure 3 6 Table 3 1 Table 5 1 Table 5 2 Table 5 3 Table A 1 Table A 2 Figure B 1 Table C 1 Table C 2 Table C 3 Table C 4 Using the MICROLAB 500 0 eeeeeeeeeeeeeeeees 3 2 Single Syringe Dispensing 3 11 DUONG a E NEE EANA thats eee 3 12 Serial Dilutions eeaeee renerne 3 13 Dual Dispensing eiseres a ees 3 14 Using the Dual Dispenser for Single Dispensing 3 15 Default Syringe Speed ValueS c ceeeeeeeees 3 6 Message Code Guide cccccccesseeceeeneeeeeeaeeees 5 2 Audible System Messages 5 4 Troubleshooting Guide ccccceseeeeeeeeeeeeeeaeees 5 5 Technical Specifications for the MICROLAB 500 A 1 Accuracy and Precision A 2 Sample Performance Test Report B 2 Reagent Diluent Syringe Replacement Parts C 1 Sample Syringe Replacement Parts 008 C 1 Valve Assemblies 000 2 eee ee cece neeeeeeeeeeeeaaeeeeeeaa C 2 TUDING PA edt evteed Auchan ee ee C 2 xi Appendix D xii Table C 5 Table C 6 Table D 1 Pipettors Probes _ Parts and Accessories Chemical Compatibility Preface Welcome to the World of Hamilton Precision Instruments Congratulations on your purchase of a Hamilton MICROLAB 500 system The Hamilton MICROLAB 500 is a versatile semi automatic precision liquid processor Various models of the MICROLAB 500 function as either single or dual syringe diluter dispensers The MICROLAB 500 function
59. ipettor Pipettor Probe Probe Probe Luer Lock Tip Push button Pipette Hand Pipettor Probe The Luer Lock Tip Push button Pipette Hand Pipettor Probe is an optional accessory that dispenses liquids through needles of different lengths and gauges This probe is useful for controlling very small dispense volumes with a high degree of accuracy It is also ideal for administering injections to small animals and for piercing septa Chapter 1 Getting Started 1 23 Viscous Sample Push button Hand Pipette Pipettor Probe The Viscous Sample Push Button Hand Pipettor Probe is an optional accessory This single dispense tube pipette is designed to accommodate highly viscous samples such as motor oil This accessory features a disposable 5 mL tip that eliminates sample carryover Air displacement ensures accurate dilutions Footswitch In addition to the hand activated probes a footswitch is also available The footswitch allows you to operate the MICROLAB 500 via a foot activated control pedal This is useful when you need to keep both hands free as when doing hand sampling Refer to Appendix C for information about ordering the footswitch or any other optional accessories Probe Button Functions The probe button on any model of hand probe serves basically the same function as the Run Stop key on the controller unit However you cannot use the probe button to pause an operation or to reinitialize the system after an error occur
60. ispense tubing to the hand probe Ifyou are using the MICROLAB 501A or the MICROLAB 503A the Concorde Probe comes as standard equipment This probe uses a single dispense tube To attach the dispense tubing into the probe loosen the knurled screw at the upper end of the probe Slide the tapered end of the dispense tube through the probe so that the tubing passes out the end of the probe When enough tubing for you to work with extends from the probe tighten the knurled screw to secure the tubing line Ifyou are using the MICROLAB 504A the Dual Hand Probe comes as standard equipment This probe is designed for both 18 and 12 gauge tubing Use the tubing reducer Part 35770 that is provided with the dual hand probe if you wish to use 18 gauge tubing See Figure 2 14 Figure 2 14 Using the Tubing Reducer with the Dual Hand Probe 7 Fill out the warranty card and return it to Hamilton Company 8 You are now ready to use your MICROLAB 500 system Chapter 2 Installing the MICROLAB 500 System 2 19 Chapter 3 Using the MICROLAB 500 System This chapter contains information about the following topics using the system an overview powering on the MICROLAB 500 and default values at start up priming and checking the system using the control keys setting syringe sizes setting syringe volumes setting syringe drive speeds sample applications Note When using the MICROLAB 500 system always take
61. ke Drive arm speeds are measured in units from 2 to 20 with 2 being the fastest speed and 20 being the slowest speed drive unit The basic body of the MICROLAB 500 Contains the electrical connectors and the syringe drive arms dual dispenser A two syringe instrument that can dispense two different liquids at the same time The volumes of the liquids being dispensed do not have to be equal The MICROLAB 504A is a dual syringe dispenser duty cycle The amount of time that an instrument is active and functioning versus the amount of time it is inactive or idle Glossary GL 3 fill To fill a syringe with a volume of liquid that is pulled in from a reservoir via fill tubing Drive arms move the syringes down pulling liquid up through the tubing and into the syringe fill tubing A tubing line that connects the liquid reservoir to the inlet valve port fluid path The inside surfaces of the valve syringes and tubing that contact liquid footswitch A foot activated device used to operate the MICROLAB 500 hand probe See probe Home position The position of the syringe drive arms when they are at the top of their range of movement inactive valve A valve with one fixed flow passage it cannot be used for liquid distribution Increase key A control used to increase the syringe size volume or speed settings initialize To establish the basic conditions for starting an operation LED Light Emitting Diode used for indi
62. lays the following message on the controller unit Size Note The actual text of this message will vary according to the language you selected using the language selection switch For more information refer to Selecting a Language in Chapter 2 This message means that the MICROLAB is ready and waiting for you to enter the sizes of the syringes that are installed on the drive unit see Chapter 2 Installing the MICROLAB 500 If you wish to run an operation you must enter a size for at least one of the installed syringes Until you enter a size only the Syringe Size keys and the Increase and Decrease keys are available for use The MICROLAB 500 will not operate without at least one syringe size value See Setting Syringe Sizes later in this chapter for information about setting syringe sizes The first time you press the Run Stop key after the system is powered on and after you have entered at lease one syringe size the system will initialize itself Until the system initializes itself Up is the only available operation x Note You can prime the instrument without entering a syringe size however you cannot run an operation without first entering at least one syringe size Chapter 3 Using the MICROLAB 500 System 3 3 Priming and Checking Prime the MICROLAB system before using it for the first time at the start of a work day and between fluid changes Run fluid through the tubing lines to ensure that there ar
63. m 3 15 Chapter 4 Caring for the MICROLAB 500 This chapter contains instructions on routine maintenance procedures for the MICROLAB 500 including the following topics deciding when to clean the MICROLAB 500 how to clean the fluid path how to clean syringes and tubing how to clean the exterior of the system how to store the system chemical compatibility of the system Note Call Hamilton Company s Technical Customer Service Department at 800 648 5950 in the United States and Canada if you have questions about maintaining your MICROLAB 500 system Deciding When to Clean the MICROLAB 500 How often you clean the MICROLAB depends upon how you use the instrument and what you use in it We recommend that if you use the instrument daily the tubing and syringes should be purged and primed at the end of each experiment and each work shift Clean the system by flushing it with Hamilton Syringe Cleaner ethanol or a with a 10 chlorine bleach and deionized water solution Avoid using caustic or acidic cleaning solutions A Important Leave the syringes and tubing filled with deionized water overnight This is important if you are using buffers or other salt solutions which could accumulate or crystallize in the system If buffers or other salt solutions are left in the fluid path overnight crystals may form and damage the syringe plunger tip A See Cleaning the Fluid Path of MICROLAB 500 for complete details on cle
64. moves the drive arms down to EJ automatically refill the syringes after liquid is dispensed When in the Auto Refill operation unlike the Up or the Down operations the syringe drive arms complete an entire cycle not just a stroke 1 20 MICROLAB 501A 503A and 504A User s Manual e Selecting the Auto Refill operation when the drive arm is in the Home position moves the drive arm to the Down position e Selecting the Auto Refill operation when the drive arm is already in the Down position moves the drive arm one full cycle from the Down position up to Home and down once again The Auto Refill operation is useful for dispense functions since it automatically refills syringes for the next dispense Therefore you can complete a fill and dispense function with one command The Auto Refill operation is also useful for doing serial dilutions Run Stop Key O The Run Stop key is located in the bottom right corner of the ip controller unit The first time you press this key after the system is powered on and the syringe sizes are selected the system will initialize itself Until the system initializes itself Up is the only active operation to move the drive arms to the Home position After initialization use the Run Stop key to start an operation Choose an operation Up Down or Auto Refill then press Run Stop to start e If Up Down or Auto Refill is selected you can pause in the middle of a stroke by pressing the R
65. must test it under the same conditions it was originally subjected to See your MICROLAB 500 s own performance test report for specific details Note When preparing to test a MICROLAB 500 system do not include the first shot dispensed after system initialization as part of the performance test Discard the first dispensed shot then start the performance test Figure B 1 shows a sample performance test report Figure B 1 Sample Performance Test Report PERFORMANCE TEST REPORT Test PASSES GRAVIMETRIC RESULTS FOR 50 Microliters Balance Sartorius Model R 160 P Product Model ML 501A Part No xXxxxx Serial NO XX XXXXXXXX DRIVE SINGLE Syringe Type 1001 Syringe Maximum Volume 1000 Microliters Syringe Dispense Volume 50 Microliters Test Method Dispensers Diluter Liquid Type H20 Liquid Temperature in Centigrade 22 Rel liquid density at Exper Temp 997801 mg microliters Actual Measurements in milligrams 49 93 mg f 50 00 49 95 mg 3 49 89 49 91 mg Z 49 83 49 91 mg 3 49 81 49 88 mg 10 49 83 RESULTS Mean Mass 49 894 mg Standard Deviation 5 929153E 02 mg Experimental Vol 50 00396 microliters Parameter Calculated Specification Pass Fail Accuracy in 0 008 1 200 PASS Co of Var in 0 0139 0 500 PASS Checked by MARIA Date 12 13 93 Signed __Maria Doe The product specified above has been calibrated at ambient pressure The calibration is performed pursuant to ML STD 45662 with an unbroken chain
66. nd then press the Run Stop key or the probe button to start the operation The system beeps once at the start of each new operation When a Down stroke completes this could be at any location within the drive arm s range of movement depending on your specific application the operation automatically changes to Up When the Up stroke completes and the syringe drive arms return to the Home position the operation automatically changes to Down If using a dual syringe instrument both drive arms must return to the Home position Otherwise Up remains the active operation When an operation completes and if no volume settings have been changed the volume settings will be reused for the next operation If the volume settings exceed the volumes remaining in the syringes the operation will not execute Instead the Exceeds Volume error message will appear in the display windows Use the Run Stop key or the Step Prime switch to reinitialize the system then enter new volume settings Press the Run Stop key to reinitialize the system after any error occurs You cannot use a probe button to reinitialize the system after an error occurs See Chapter 5 for more information about system messages and error recovery Pausing Operations If Up Down or Auto Refill is selected you can pause the instrument during a stroke by pressing the Run Stop key once Resume the operation by pressing the Run Stop key again To cancel a paused operation pres
67. nother fluid The fluid that is added is called a diluent and may be deionized water for example diluter An instrument used to reduce the concentration of a liquid The MICROLAB 503A is a dual syringe diluter dispense To distribute fluid in a single portion or in several increments A diluent is dispensed during dilution Reagents and buffers can be mixed and dispensed together with a sample before analysis dispense tubing Tapered tubing that is attached to a hand or foot probe to deliver precise amounts of liquid GL 2 MICROLAB 501A 503A and 504A User s Manual dispenser An instrument used to distribute liquid The MICROLAB 501A is a single syringe dispenser The MICROLAB 504A is a dual syringe dispenser disposable tip A throw away probe tip used to avoid cross contamination between samples display windows The two areas on the controller unit where system messages and information appear Synonymous with message display windows Down Describes the movement of the syringe drive arm as it moves away from the Home position and stops at the bottom of the downward stroke dribbling Describes the formation of drops at the tip of tubing as liquids are dispensed Generally occurs when small volumes are dispensed at low flow rates or when there is an air leak in the tubing lines Drop formation can adversely affect accuracy and precision drive speed Speed of the drive unit speed is specified in seconds per full stro
68. play Right Side Display Meaning Action Size Size This message appears before initialization By default the size prompt appears in the left display panel The system is prompting you to enter a syringe size If you are using a single syringe unit enter the syringe size If you are using a dual syringe unit enter the size of the left syringe or press the right syringe size selection control to enter a size for the right syringe Spd xx Spd xx The system displays the current default speed setting where xx represents a speed from 2 to 20 Unit Paused This message appears when the Run Stop key or the probe button is pressed during an operation to pause the instrument The prime switch is inactive when the unit is paused continued MICROLAB 501A 503A and 504A User s Manual Table 5 1 Message Code Guide continued Left Side Right Side Meaning Action Display Display Syr Stall This message appears only on single syringe units The syringe is stalled R Syr Stall This message appears only on dual syringe units The right syringe is stalled L Syr Stall This message appears only on dual syringe units The left syringe is stalled Val Stall This message appears only on single syringe units The valve is stalled R Val Stall This message appears only on dual syringe units The right valve is stalled L Val Stall This mess
69. rectly above the power indicator light See Figures 1 1 through 1 3 The Step Prime switch is a three position rocker switch e When the Step Prime switch is in the middle position the ML500 is inactive 1 14 MICROLAB 501A 503A and 504A User s Manual e When you press the lower part of the Step Prime switch the ML500 goes into prime mode When in prime mode the syringe drive arms automatically move up and down opening the valve ports and moving fluid through the system The ML500 stays in prime mode until you press the upper or the middle part of the Step Prime switch e When you press the upper part of the Step Prime switch the ML500 goes into Step mode When in Step mode the drive arms move only as long as you depress the Step Prime switch gt 4 When you release the switch movement stops Use the Step mode to move the syringe drive arm down and away from the home position before installing or removing syringes Note Use the Step mode to return the syringes back to the Home position This good habit ensures that the syringes will be in the correct position for your next command Valve Assembly The valve assembly controls the flow of liquid through the fluid path The 501A 503A and 504A models each use a different valve assembly you must attach the assembly to the drive unit See Figures 1 1 through 1 3 for the location of the valve assembly See Installing the Valve Assembly in Ch
70. s 1 24 MICROLAB 501A 503A and 504A User s Manual Chapter 2 A Important Installing the MICROLAB 500 System This chapter contains information about installing the MICROLAB 500 system including the following topics an overview of installation procedures how to select an installation location how to install the accessory holder how to select the language system messages will display in how to install electrical connections how to install the valve assembly how to select install and remove syringes how to select and install tubing Be sure to read the instructions in this chapter before installing your MICROLAB system Never install or use syringes incorrectly Incorrect use may result in damage to the syringes See Selecting Installing and Removing Syringes later in this chapter for more information Never over tighten tubing Over tightening may result in damage to the valves or tubing See Selecting and Installing Tubing later in this chapter for more information Call Hamilton Company s Technical Customer Service Department at 800 648 5950 in the United States and Canada if you have questions about installing your MICROLAB 500 system A Figure 2 1 Overview of Installation Procedures Unpack the instrument check all parts against packing list v Select an installation location Y Install the accessory holder y Set the language s
71. s any key except the Run Stop key If you cancel an operation and then press the Run Stop key the drive arms will move to the Home position If the Step Prime switch on the drive unit is in use and is in the Prime position you cannot use the Run Stop key to pause during a stroke or a cycle Instead pressing Run Stop causes the drive arms to continue their movement until they reach the Home position Then they stop You cannot use a probe button to pause an operation 3 10 MICROLAB 501A 503A and 504A User s Manual Sample MICROLAB 501A Application Configurations Single Syringe Dispensing When the probe button is pressed once fluid is pulled from the reservoir into the TLL syringe The valve then switches to connect the fluid path from the TLL syringe to the hand probe When the probe button is pressed a second time liquid is dispensed out of the probe tip Figure 3 2 shows the Concorde Probe being used for single syringe dispensing Figure 3 2 Single Syringe Dispensing R ll O Diluent Chapter 3 Using the MICROLAB 500 System 3 11 Sample MICROLAB 503A Application Configurations Example 1 Dilutions When the probe button is pressed once the syringe drive arms move down pulling diluent in from the reservoir and aspirating sample into the probe tip Then the valv
72. s on the principal of liquid liquid displacement At the heart of each MICROLAB 500 system is a highly efficient precision stepper motor drive that is combined with world famous Hamilton GASTIGHT syringes The result is a precise and accurate instrument that is very easy to set up and use With proper care and maintenance your new MICROLAB 500 system will serve you faithfully To learn about the proper care and maintenance of your investment please take the time to read this manual Also please read the warranty information that appears on the copyright page in this manual and on the separate warranty sheet that is included in your MICROLAB 500 shipping kit The Hamilton Company thanks you for purchasing this Hamilton product Welcome to the world of Hamilton precision instruments About the MICROLAB 500 Series of Instruments All of the MICROLAB 500 systems feature four common pipette modes fill dispense auto refill and prime The systems are capable of performing accurate and precise transfer pipetting and of performing automated dilutions up to 1 25 000 The instruments can also dispense up to 50 mL per cycle Figure PR 1 provides an overview of the MICROLAB 500 Series of Instruments Figure PR 1 The MICROLAB 500 Series of Instruments The Microlab 500 Series of Instruments Single Syringe Instruments Dual Syringe Instruments Controller without memory ere a ae ae Microlab 501A dispenser e Microlab 504A dispenser
73. small green light next to the key lights up to show you that the key is active To increase a syringe size press the Increase key to decrease a syringe size press the Decrease key As you press either of these keys the Syringe Size Indicator lights move to indicate the selected size and the size itself appears in the Message Display Windows As you press the Increase Decrease key the displays change continuously When either the highest or the lowest size limit is reached the displays automatically wraps around enabling you to quickly select the correct size for your needs When setting syringe sizes you can choose from 10 different sizes that correspond to Hamilton syringes 25 uL 50 uL 100 uL 250 uL 500 uL 1000 uL 2500 uL 5000 uL 10 000 uL and 25 000 uL The default value is 1000 uL Note Above 500 uL the Syringe Size Indicator light shows the syringe size in milliliters while the display window shows the same size in microliters When you reach the size you need release the Increase or the Decrease key Verify your selection by checking the size that appears in the message display windows If you are using a dual syringe instrument repeat steps 1 through 4 for the second syringe Chapter 3 Using the MICROLAB 500 System 3 7 Setting Syringe Volumes You can specify a volume only after you have selected and entered a size for the corresponding syringe To set a volume follow these steps 1 3 8
74. stem clears the fluid path of the deionized water that you used as a rinse 3 4 MICROLAB 501A 503A and 504A User s Manual 10 When the fluid path is free of deionized water place the inlet tubing into a reservoir of the liquid you wish to dispense or use as a diluent Prime this fluid through the system Note When priming reagent after a deionized water rinse dispense the first shot into a waste container since it will contain a certain amount of the water droplets that remained in the tubing line Your MICROLAB system is clean and ready for use Important When priming the MICROLAB 504A dual dispenser always prime both syringes at the same time or set the volume of the unused side to zero Do not operate either syringe dry as this may cause excessive wear on the syringes and valves A Default Values The MICROLAB 500 assumes certain default values when it is powered on The system uses these speeds unless you change the speed of the syringe drive arm by using the Syringe Speed keys and the Increase or the Decrease key The Syringe Size key is active by default with a value of 1000 mL If you are using a dual syringe instrument the left side Syringe Size control is active You must enter a size for at least one of the installed syringes before you can operate the instrument If you are using a dual syringe instrument and do not enter a size for the second syringe it remains inactive Syringe Volume defaults to 100
75. sure that neither bubbles nor air gaps exist in the tubing lines You must prime the system prior to use and between fluid changes prime mode The process of priming an instrument probe A hand held device that contains dispense tubing Each probe also has a button that can be used to control the drive unit reagent diluent syringes Syringes that pick up and dispense reagent and diluent The GASTIGHT TLL type and TLLX type syringes are reagent diluent syringes reservoir A container that holds system fluid diluent Returned Goods Authorization Number RGA A number assigned by the Hamilton Company that is used to trace your instrument in the event it is returned for repair Run Stop key A control used to start stop or pause an operation on the MICROLAB 500 sample syringe A syringe used to pick up a liquid sample GASTIGHT D configuration syringes with side ports are sample syringes On a dual syringe unit the sample syringe is usually placed on the instrument s right side Speed key A key located on the controller unit used to select the speed function step Movement of the drive arms that occurs when the Step Prime switch is held down The Step mode is often used to position the drive arm before changing syringes GL 6 MICROLAB 501A 503A and 504A User s Manual stroke Full movement of the syringe drive arm in one direction only half of the drive arm s cycle See also cycle syringe drive arm s Th
76. syringe in the left position TLL type or TLLX type syringes e The MICROLAB 504A uses two dispenser diluent type syringes one each in the left and right positions TLL type or TLLX type syringes e The MICROLAB 503A uses one reagent diluent syringe in the left position and one sample syringe in the right position DX type sample syringe e Asa general rule to ensure high accuracy and precision try to use 10 or more of a syringe s total volume Consider using a different size syringe if your application calls for driving a syringe less than 10 of its total volume and high accuracy and precision are required Table 2 2 Reagent Diluent Syringes TLL and TLLX types Syringe Sizes Model Number Part Number Optimal Ranges HL 25 uL 1702 TLLX 80222 2 5 25 50 uL 1705 TLLX 80922 5 50 100 uL 1710 TLLX 81022 10 100 250 uL 1725 TLLX 81122 25 250 500 uL 1750 TLLX 81222 50 500 1mL 1001 TLLX 81323 100 1000 2 5 mL 1002 TLL 81420 250 2500 5mL 1005 TLL 81520 500 5000 10 mL 1010 TLL 81620 1000 10 000 25 mL 1025 TLL 82521 2500 25 000 2 8 MICROLAB 501A 503A and 504A User s Manual Table 2 3 Sample Syringes DX type Syringe Sizes Model Number Part Number Optimal Ranges uL 25 uL 1702 DX 80226 2 5 25 50 uL 1705 DX 80926 5 50 100 uL 1710 DX 81026 10 100 250 uL 1725 DX 81126 25 250 500 uL 1750 DX 81226 50 500 1mL 1001 DX 81326 100 1000
77. t to the key lights up to show you that the key is active To increase or decrease the Speed press the Increase key or press the Decrease key As you press the Increase Decrease key the display changes continuously When either the highest or the lowest speed limit is reached the display automatically wraps around enabling you to quickly select the correct speed for your needs Choose a drive speed anywhere from 2 to 20 seconds per full stroke Note Use slower drive speeds when pipetting large volumes and or viscous or volatile liquids Slower drive speeds improve accuracy and precision When you reach the desired speed release the Increase or the Decrease key Verify your selection by checking the speed that appears in the message display windows If you are using a dual syringe instrument repeat steps 1 through 4 for the second syringe Running Operations When you press the Run Stop key the first time after the system is powered on and after you have entered syringe sizes the system initializes itself That is the drive arms move up to the Home position Until the system initializes itself Up is the default operation After initialization Down becomes the default operation Chapter 3 Using the MICROLAB 500 System 3 9 After you have entered any required values for the control keys and the system has initialized itself you may select an operation Down or Auto Refill Press the desired operation key a
78. tailed information such as technical specifications ordering information etc The Glossary defines terms used in this manual The Index provides a quick reference to the topics described in this manual PR 4 MICROLAB 501A 503A and 504A User s Manual Conventions Used in This Manual Throughout this manual symbols are used to call your attention to various kinds of information A Warning Information that is essential for avoiding personal injury is flagged with the international Warning Symbol and appears like this in the text A A Important Information that is essential for avoiding damage to equipment appears like this in the text A Note Interesting information or information that can help improve system performance appears like this in the text System messages or prompts that appear in the message display windows are shown in courier font A Word About Single Syringe Instruments Throughout this manual you will see references to multiple syringes volumes speeds and to the right side controls If you are using a MICROLAB 501A single syringe dispenser please disregard these references The right side controls are not functional on single syringe instruments The right display window however is active Any operational differences between the single and the dual syringe instruments are called out in the text Preface PR 5 Chapter 1 Getting Started This chapter provides a brief overview of the MICROL
79. to MICROLAB 504A systems For upgrade information or for information about purchasing any of the MICROLAB 500 models contact your authorized Hamilton sales representative or contact Hamilton Company In the United States Hamilton Company P O Box 10030 Reno Nevada 89520 0012 Telephone Numbers in the USA and Canada Technical Customer Service 1 800 648 5950 8 a m to 5 p m PST Instrument Service 1 800 527 5269 Outside the USA and Canada 1 775 858 3000 Fax Number 1 775 856 7259 Preface PR 3 In Switzerland Hamilton Bonaduz AG Ch 7402 P O Box 26 Bonaduz Switzerland Telephone Number 41 81 660 60 60 Fax Number 41 81 660 60 70 About This Manual This manual provides technical information about the MICROLAB 501A 503A and 504A and is divided into chapters that cover the following topics Chapter 1 Getting Started provides an overview of the MICROLAB 500 system including a complete parts list and a brief description of the system components Chapter 2 Installing the MICROLAB 500 describes how to set up the system Chapter 3 Using the MICROLAB 500 provides step by step instructions for using the system It also provides sample applications Chapter 4 Caring for the MICROLAB 500 describes everyday maintenance techniques Chapter 5 Troubleshooting contains tables that list system messages and their meanings and common problems and their solutions The Appendixes provide de
80. ton Bonaduz AG Ch 7402 P O Box 26 Bonaduz Switzerland Telephone Number 41 81 660 60 60 Fax Number 41 81 660 60 70 Chapter 5 Troubleshooting 5 9 Returning Instruments for Repair Before returning an instrument to Hamilton Reno for repair notify Hamilton Company s Instrument Service Department at 800 527 5269 in the United States and Canada and request a Returned Goods Authorization Number RGA number Note Do not return instruments to Hamilton Company without an RGA number This number assures proper tracking of your instrument Instruments that are returned without an RGA number will be sent back to the customer without being repaired Important Important Important Decontaminate the instrument and remove health hazards such as radiation infectious diseases corrosive agents etc Provide a complete description of any hazardous materials that have been used with the instrument A Hamilton Company reserves the right to refuse a return shipment of any Hamilton product that has been used with radioactive or microbiological substances or any other material that could be hazardous to Hamilton employees A Do not return syringes tubing or valves with your instrument Hamilton Company will assume that they pose a health hazard and will destroy them A 5 10 MICROLAB 501A 503A and 504A User s Manual Appendixes Appendix A Technical Specifications for the MICROLAB 500 Appendix A
81. un Stop key once Resume an operation at the same place in the stroke by pressing the Run Stop key again e If the Step Prime switch on the drive unit is in use and is in the Prime mode you cannot use the Run Stop key to pause in the middle of a stroke or a cycle Instead the drive arms continue until they reach the Home position before they stop The Run Stop key generates a command signal that activates the next step of an operation You can generate this same command signal by pressing the button on a hand probe or by using a footswitch See Chapter 3 Using the MICROLAB 500 for complete instructions about using the function keys Chapter 1 Getting Started 1 21 Pipettors Probes Each MICROLAB 500 system comes with a remote push button hand held pipettor probe The standard accessory probes include e the Concorde Push button Hand Pipettor Probe ships with the MICROLAB 501A and the MICROLAB 503A e the Dual Push button Hand Pipettor Probe ships with the MICROLAB 504A Additionally three other optional probes may be ordered separately for use with any MICROLAB 500 system These probes include e the Disposable Tip Push button Hand Pipettor Probe e the Luer Lock Tip Push button Pipette Hand Pipettor Probe e the Viscous Sample Push button Hand Pipettor Probe Concorde Push button Hand Pipettor Probe The Concorde Push button Hand Pipettor Probe is a single dispense pipette probe that features a slim comfortable design
82. ve up dispensing the two liquids out of separate tubing lines Figure 3 5 shows the Dual Hand Probe being used for dual dispenses Figure 3 5 Dual Dispensing Coo mmi Diluent Diluent Example 2 Using the Dual Dispenser for Single Dispensing MICROLAB 504A Example 2 follows the same procedures as those shown in the MICROLAB 504A Example 1 except that liquid is dispensed out of the right side only On the left side a liquid is cycled from the reservoir through the fluid path and back again simply to lubricate the fluid path Figure 3 6 shows the Concorde Hand Probe being used for single dispensing 3 14 MICROLAB 501A 503A and 504A User s Manual Figure 3 6 Using the Dual Dispenser for Single Dispensing SS gt Diluent TAT y Tyr y Cycle back to Dispense from reservoir single reservoir Note After the syringe size is set the right or left syringe can remain idle if the fill and the dispense volume for that syringe is set to 0 zero Do not operate either syringe dry as this may cause excessive wear on the syringes and valves Chapter 3 Using the MICROLAB 500 Syste
83. vitation is occurring syringe drive speed is too high for the current liquid e loose worn or incorrect tubing fittings e damaged syringe plunger tip e damaged valve e reduce syringe drive speed use lower drive speeds for viscous liquids e hand tighten the fittings or replace old tubing with new or correct size tubing e replace plunger tip or syringe e replace valve unit is overheated e inadequate ventilation room temperature too high or duty cycle too high e the unit is overheated power it off and allow it to cool down e resume use at a lower duty cycle e see Appendix A Technical Specifications for information about appropriate environmental conditions 5 8 MICROLAB 501A 503A and 504A User s Manual Getting Technical Support If a problem persists even after you have attempted to correct it contact the Hamilton Company Instrument Service Department To expedite service please have your instrument s model and serial number ready and available Also please provide application specific information such as syringe sizes drive speeds and liquids In the United States Hamilton Company P O Box 10030 Reno Nevada 89520 0012 Telephone Numbers in the USA and Canada Technical Customer Service 1 800 648 5950 8 a m to 5 p m PST Instrument Service 1 800 527 5269 Outside the USA and Canada 1 775 858 3000 Fax Number 1 775 856 7259 In Switzerland Hamil
84. witch Use the Language Selection switch to choose the language that message codes will appear in Messages codes provide you with system status and appear in the message display windows located on the controller unit You can select from five different languages including English French German Portuguese and Spanish Chapter 1 Getting Started 1 13 Controller Unit Connector Receptacle The controller unit connector receptacle is located on the back of the drive unit just below the serial number plate Insert the controller unit cable connector into this receptacle the cable fits only one way See Figure 1 4 for the location of the controller unit connector receptacle Power On Off Switch and Power Indicator Light The Power On Off switch and the Power Indicator light are located on the front of the drive unit in the lower right hand corner See Figures 1 1 through 1 3 for the locations of the switch and the indicator light The Power On Off switch is a two position rocker switch e To power on the MICROLAB 500 press the upper half of the switch The system beeps twice when it is powered on e To power off the MICROLAB 500 press the lower half of the switch The Power Indicator light is a small green LED located directly above the Power On Off switch It is lit when the unit is powered on Step Prime Switch l The Step Prime switch is located on the front of the drive unit in a the lower right hand corner di

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