Home

Biomek NX and NXP Laboratory Automation

1. syringe size Minimum Cutorn Maximum Cutoff Velocity Velocity 100 uL 0 833 uL second 45 0 uL second 250 uL 2 08 uL second 112 5 uL second 500 uL 4 17 uL second 225 uL second 1 mLE 8 33 uL second 450 uL second 2 5 mL 20 8 uL second 1125 uL second 5 mL 41 7 uL second 2250 uL second a Recommended cutoff velocity is 100 uL second b Recommended cutoff velocity is 150uL second c Recommended cutoff velocity is 150 y second NOTE The minimum Cutoff Velocity for each syringe is established using the following equation 50 x lt Syringe size in UL gt 6000 The maximum Cutoff Velocity for each syringe is established using this equation 2700 x lt Syringe size in uL gt 6000 Optimizing Syringe Performance and Life Expectancy To achieve optimal performance and maximum life expectancy syringes should be used cleaned and stored in accordance with the manufacturer s specifications Do not run syringes more than a few cycles without fluid in the syringes Syringes should not actuate while dry unless the system is being primed refer to Priming the System with System Fluid When priming the system the syringes should cycle at maximum volume as few times as possible in order to flood the syringe with system fluid as quickly as possible NOTE Acycle is one up or down motion of the plunger in a syringe Refer to Assembling and Installing Syringes for identification of the parts of a syringe Liquid Syste
2. Choose OK The gripper moves to the selected position and Align Gripper Rotation appears Figure 3 34 Figure 3 34 Align Gripper Rotation Align Gripper Rotation x Click either the right or left button to rotate the gripper Once the gripper is centered at 0 degrees press OK Move in fi degree T increments S Rotate Right In Move in degree increments enter how far the gripper should rotate with each movement Choose Rotate Left to rotate the gripper to the left OR Choose Rotate Right to rotate the gripper to the right 987887AE Framing Instructions Troubleshooting 10 Repeat steps 8 and 9 until the gripper is centered at 0 degrees NOTE Centering the gripper is a visual task 11 Choose OK to complete the rotation calibration 12 Choose Accept to save the calibration and close Hardware Setup d Troubleshooting 987887AE Perform the troubleshooting techniques provided in Table 3 1 when necessary NOTE In the case of any other framing related problems contact a Beckman Coulter Representative Table 3 1 Troubleshooting Framing If Then AccuFrame power light not on Check the CAN connection to make sure the AccuFrame is connected to Biomek NX instrument The Y axis and the X Z axes Light Beams cannot be broken when moving a finger around the interior of AccuFrame Make sure the AccuFrame is receiving power One or both beam indicator lights on AccuFrame
3. Start This wizard assists with determining the maximum sensitivity for each probe This will help minimize errrors created by receiving a false Load Tips detection Choos All of the probes will need to have tips loaded Any probe configured to use disposable tips will have to be loaded with a conductive tip Update Settings The current tip configuration is Probe 1 Disposable tip No tip configured Probe 2 Disposable tip No tip configured Probe 3 Disposable tip No tip configured Probe 4 Disposable tip No tip configured Probe 5 Disposable tip No tip configured Probe 6 Disposable tip No tip configured Probe 7 Disposable tip No tip configured Probe 8 Disposable tip No tip configured Cancel Load Tips will not be displayed on the left side if all probes are configured with fixed tips NOTE On the left side of Find Sensitivities a list of steps required to complete the procedure is displayed As the steps of Find Sensitivities are accessed they are highlighted on the left NOTE ASpan 8 Pod that is configured with all fixed tips will not display Load Tips on the left side of Find Sensitivities Figure 2 11 Find Sensitivities Start Tip Detection Start This wizard assists with determining the maximum sensitivity for each probe This will help minimize errrors created by receiving a false Load Tips detection Choos All of the probes will need to have tips loaded Any prob
4. Calibrating Gripper Rotation The gripper can rotate to access positions that are oriented at different angles in relation to the front of the Biomek NX instrument however controlling this feature in Biomek Software in a method is available only for custom integrations To ensure that the gripper rotates properly they need to be calibrated to a 0 degree rotation To calibrate the gripper rotation 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software to start Biomek Software 2 From the Instrument menu choose Hardware Setup Hardware Setup appears 3 Select Pod to display the configuration information for the pod 987887AE Framing Instructions 3 Calibrating Gripper Rotation 4 Choose Gripper Settings to expand the settings The configuration for the gripper appears on the right Figure 3 31 Figure 3 31 Hardware Setup Biomek Hardware Setup B Reconnect A Home All Axes Add Device Remove Device J Accept gt lt Cancel Biomek NX SN None Probe Configuration A oe ee a tiFrams E E E ETET Save Settings Restore Settings s Delete Settings gt et ee paT Devices oF DeviceControllerO Axis Limit Settings 47 ShakerALPO X cm Y cm Zem Duly Span cm Minimum 2 11 ss 15 938 fo fea gt Sti Maximum 102 79 fo Jeserese ooo fia g StirrerALP1 47 Tiploader0 Probes 1 4 use eK Sev 7 Tiploader1 Disposable gt tips
5. OR Choose Output to open the valve and allow system fluid to flow to and from the syringe with the tip as the source destination of the fluid OR Choose Purge to open the valve and allow system fluid to flow through the valve without activating bypassing the syringe 7 Choose Done after the valves have been set as desired Valve Settings closes 8 Choose Close to close Advanced Manual Control Choose Exit to close Manual Control Verifying Liquid Level Sensing 4 16 It may be useful to verify the operation of liquid level sensing Use LLS Z Move to allow probes to descend and then stop once they hit liquid To verify Liquid Level Sensing 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the Span 8 Pod appears Figure 4 7 4 Check LLS Z Move LLS Z Move can be found under Delta 5 In Movement Vector enter the maximum Z distance each probe should descend NOTE Use Advanced Manual Control Figure 4 7 to disable any probes that should not descend Check marks next to the probe number indicate the probe will descend when Go is chosen 6 Choose Go 987887AE Manually Controlling the Biomek NX in Biomek Software 4 Using Advanced Manual Control with Span 8 Pod and Gripper 7 Choose Close to close Advanced Manual Control 8 Choose Exit to close Manual Control Purging Air from the Syringes and Tubing
6. Amount of time in milliseconds the system waits after purging air and liquid to Post Porge Delay the Span 8 Tip Wash ALP Amount of liquid in milliliters that is dispensed to the reservoir of the Span 8 Dispense Tip Wash ALP Amount of liquid in milliliters that is used to wash the tips of the Span 8 Tip Wash Wash ALP in the eight cleaning wells When checked uses the speed pump to bypass the pump valves to accelerate Use Speed Pump the speed of the system fluid as it passes through the tubing and is dispensed from the tips into the Span 8 Tip Wash ALP Setting Span 8 Gripper Properties If the Biomek NX Span 8 is equipped with a gripper tool for moving labware gripper properties in Hardware Setup are initially configured by a Beckman Coulter Representative and should not be modified without specific instructions from a Beckman Coulter Representative However gripper settings should be adjusted when physical changes have been made to the Biomek NX Span 8 instrument Always consult a Beckman Coulter Representative before changing values Table 2 4 describes the gripper settings listed in Hardware Setup for a Span 8 Pod equipped with a gripper tool The fields listed in the following table may be accessed by choosing Additional Pod Settings in Hardware Setup 987887AE 987887AE Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Table 2 4 Span 8 Gripper Settings Propert
7. NOTE If tips have been configured to unload they will unload now 12 Choose Finish Find Clot Detection Sensitivities closes 13 Choose Accept Hardware Setup closes N Setting Span 8 Pod Properties Most of the properties including the settings and axes limits of the Span 8 Pod are initially configured in Hardware Setup by a Beckman Coulter Representative and should not be modified without specific instructions from a Beckman Coulter Representative However pod properties should be adjusted when physical changes have been made to the Biomek NX instrument Always consult a Beckman Coulter Representative before changing values 2 22 987887AE Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Do not make any changes to the axes limits or pod settings without consulting a Beckman Coulter Representative Table 2 2 describes the pod properties and axes limits listed in Hardware Setup for a Span 8 Pod NOTE Some of the fields listed in the following table may be accessed by choosing Additional Pod Settings in Hardware Setup Table 2 2 Span 8 Pod Properties Property Description Additional Roving Height Margin above the default height for the pod as it moves over everything on the deck Additional Timeout Specifies the number of seconds to wait in addition to the normal time it takes to carry out a step before a timeout error occurs Use when experiencing timeout problems with
8. Probe Tip Interface Fixed Tip Collar A WN 3 Gently pull the tubing and tip down from the Span 8 probe until enough tubing is extended to grasp between thumb and forefinger Figure 1 12 987887AE 1 21 Span 8 Pod Interchangeable Tips 4 while holding the tip in one hand pull the tubing off the tip with the other hand Figure 1 13 NOTE Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping while pulling the tubing off the tip NOTE Carefully twisting the fixed tip while pulling on the tubing may ease removal of the tip Figure 1 13 Removing a Fixed Tip Probe Tip Interface Tubing Fixed Tip PWN 5 Repeat steps 1 through 4 to remove the remaining tips N Disposable Tips Mandrels Disposable tips and LLS disposable tips can be used on the Span 8 Pod To use the disposable tips option disposable tip mandrels must be installed on the Span 8 probes and configured for use in the Hardware Setup 1 22 987887AE Span 8 Pod Interchangeable Tips Installing Disposable Tip Mandrels Do not perform aspirate or dispense operations without mandrels installed and tubing attached to disposable tips To operate the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tip mandrels
9. To frame deck positions of the Biomek NX using AccuFrame a framing fixture is attached to the head of the Multichannel Pod a framing shaft is attached to a probe of the Span 8 Pod When framing multiple position ALPs such as the 1 X 3 Passive ALP all positions on the ALP should be framed to increase precision To frame the deck positions of the Biomek NX the following operations must be completed Homing All Axes of the Pods e Attaching the Framing Fixture to the Pod e Installing AccuFrame 987887AE Framing Instructions 3 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame e Framing the Position NOTE Some ALPs require additional or slightly modified procedures to frame properly Consult the ALPs User s Manual PN 987836 for the specific ALP to frame for any special instructions NOTE Deck positions on the Biomek NX can also be framed by choosing Manual Teach on Position Properties Manual Teach involves either loading tips and visually aligning them with the wells on a piece of labware or moving a piece of labware from a framed position to the position to be framed refer to Manually Framing Deck Positions on the Biomek NX Instrument Manual Teach is useful if specialized or very high density labware is used or when Auto Teach yields unsatisfactory results Some ALPs such as the Span 8 Tip Wash ALP and the Positive Position ALP must be framed manually Homing All Axes of the Pods Before selecting OK to h
10. f Digital Devices ea i X orrelate Pods orrelate 2 Axes ind LLS Sensitivities 5 Simulator 1ml syringes Gorrelate Pod Gorrelate 2 Find LUS Sensitivities OF Camera Disabled Probes OP Materials 1 2 3 4 5 e 7 8 Pump lt None gt 7 Find Gb Benstivtes fA Stacker Carousels Additional Pod Settings Gripper Settings IV Gripper Installed x cm cm Z cm Grip em etect Side Collisions Offset 7 783 12 692 F 16 4 fo Rotation Calibration IV Front Minimum fo f 29 81 fo IV Back Maximum 43 47 fe 3 fe 19 IV Right J Set Y Grip JZ Enable Plate Sensor I Left BiomekNX Span 5 Choose Rotation Calibration A Warning appears Address the topics that appear in the Warning and choose OK Figure 3 32 Figure 3 32 Warning to Make Sure Gripper is Ready for Framing x The grippers cannot be holding anything For this operation oe make sure there is nothing between the pod and the location that you will calibrate the gripper at such as a stack of i Cancel 987887AE 3 35 Framing Instructions Calibrating Gripper Rotation 6 In Pick Position Figure 3 33 select a deck position to move the gripper over such that it will be easy to visually observe the gripper Figure 3 33 Pick Deck Position to Calibrate the Gripper Pick Position x Place the framing tool on a position that has been framed select that position below and hit Ok
11. 2 Click Yes to delete Settings1 Click No to return to Hardware Setup without deleting No 5 Choose Yes The stored configuration is deleted 987887AE 2 29 Configuring the Biomek NX Span 8 in Biomek Software Saving Restoring and Deleting Settings 2 30 987887AE CHAPTER 3 Framing Instructions _ Overview Framing is the procedure of teaching Biomek Software the location of the ALPs and devices positioned on the deck Biomek Software uses this framing information to move the pod or gripper to the appropriate position to perform liquid handling operations and manipulate labware A Beckman Coulter Representative frames the Biomek NX instrument during system installation It may be necessary to repeat the framing if ALPs or devices are added moved or removed from the deck the probes on the Span 8 Pod are changed Framing deck positions can be performed automatically using the AccuFrame framing tool or manually using a piece of labware to visually align the pod to the wells The gripper if installed may also be framed using the AccuFrame framing tool Framing the Biomek NX instrument includes e Framing Deck Positions on the Biomek NX Instrument Using AccuFrame Manually Framing Deck Positions on the Biomek NX Instrument e Framing the Span 8 Gripper e Calibrating Gripper Rotation Framing Deck Positions on the Biomek NX Instrument Using AccuFrame The AccuFrame is a tool used for fr
12. Probe Tip interface Disposable tip mandrel Collar 1 2 3 4 1 24 987887AE Span 8 Pod 1 Interchangeable Tips Do not allow the tip mandrel to twist while fastening or removing the collar The collar contains an O ring which turns the tip mandrel if not secured causing the tubing to get twisted or kinked 5 Hold the tip mandrel firmly in place while screwing the collar Screw the collar clockwise into the tip interface until a tight fit is achieved NOTE Each week check that disposal tip collars are securely fastened to the tip interface Disposal tips may not shuck if collars are loose 6 Pass the tip shuck tube up over the disposable tip mandrel to the tip interface Figure 1 16 Figure 1 16 Installing a Tip Shuck on a Disposable Tip Mandrel Probe Tip interface Disposable tip mandrel Tip shuck tube PWN 987887AE 1 25 Span 8 Pod Interchangeable Tips Screw the tip shuck tube clockwise into the tip interface until a tight fit is achieved Make sure the tip shuck tube is securely tightened to prevent it from working off during operation The disposable tip mandrel is now installed Figure 1 17 Figure 1 17 Installed Disposable Tip Mandrel Probe Tip Interface Tip Shuck Tube Disposable Tip Mandrel PWN 8 Repeat steps 1 through 7 for any other probes requiring disposable tips N 1 26 987887AE Span 8 Pod 1 Interchangeable Tips Removing the Disposable T
13. 1 1 Overview 1 1 Main Components of the Span 8 Pod 1 2 Pod Movements 1 3 Probes 1 4 Interchangeable Tips 1 4 Liquid Level Sensing 1 5 Liquid Level Sensing Tips 1 5 Liquid Level Sensing Settings 1 5 Disabling Liquid Level Sensing 1 5 Clot Detection 1 6 Clot Detection Settings 1 6 Clot Detecting Tips 1 6 Disabling Clot Detection 1 7 Selecting Which Type of Tips to Use 1 7 Labware and Tip Compatibility with the Span 8 Pod 1 8 Electro Static Discharge Protection when Handling Tips on the Span 8 Pod 1 13 Fixed Tips 1 14 Installing Fixed tips 1 15 Removing Fixed Tips 1 19 Disposable Tips Mandrels 1 22 Installing Disposable Tip Mandrels 1 23 Removing the Disposable Tips Option 1 27 Pump Assembly 1 32 Pumps 1 32 Syringes 1 33 How Syringes Affect Pipetting Accuracy 1 33 Syringe Size 1 34 Assembling and Installing Syringes 1 34 Removing and Disassembling Syringes 1 37 Syringe Cutoff Velocities 1 39 Optimizing Syringe Performance and Life Expectancy 1 40 Liquid System 1 40 System Fluid 1 41 Using Non Supported System Fluids 1 41 Tubing 1 43 Supply Container 1 44 Exchanging Supply Containers 1 45 Waste Container 1 48 Exchanging a Waste Container 1 49 Preparing the Liquid System 1 51 Contents Degassing the System Fluid 1 51 Priming the System with System Fluid 1 51 Purging the Tubing and Syringes of Air 1 52 Purge Pump 1 52 Gripper 1 52 Framing the Gripper 1 54 Preve
14. 1 55 control modes overview 0 xxv Correlating the Z Axes in Hardware Setup 2 10 cutoff velocity defined 1 33 equation to determine 1 40 maximum 1 40 minimum 1 40 of syringes 1 39 D deck overview 0 xxix Delta setting values for the Span 8 Pod 4 11 delta values Index 1 Index Index 2 using in Manual Teach 3 19 3 27 Device Controller overview 0 xxix devices optional overview 0 xxix disposable tips installing disposable tip mandrels 1 23 removing disposable tip mandrels 1 27 E ESD protection 1 13 F Find CD Sensitivities performing 2 17 Find Sensitivities performing 2 11 firmware version viewing 4 5 fixed tips removing 1 19 framing using delta value 3 19 3 27 using graphic alignment tool 3 18 3 25 framing teaching instructions 3 1 Stacker Carousel B 29 using Manual Teach 3 11 framing shaft attaching to a probe of a Span 8 Pod 3 4 fuses changing in tower 0 xxxiii G graphic alignment tool using during framing 3 18 3 25 gripper calibrating rotation 3 34 defined 1 52 detect side collisions 2 25 enabling plate sensor 2 25 framing 3 28 Set Grip 2 25 Set Y 2 25 using to manually frame 3 23 grippers testing framing accuracy 3 34 H Hardware Setup accessing 2 1 choosing Frame Gripper 3 29 configuring a Span 8 Pod in 2 3 configuring plate sensor 2 25 configuring the Biomek NX in 2 3 configuring the probes in 2 4 correlating th
15. Changing from fixed tips to disposable tips e Changing the type of fixed tips When probe configuration is changed from disposable to fixed tips or from fixed to disposable tips the pod must be homed refer to CHAPTER 3 Homing All Axes of the Pods and Find Sensitivities must be performed refer to Performing Find LLS Sensitivities NOTE The pod may be homed by choosing Instrument gt Hardware Setup gt Home All Axes from Biomek Software NOTE The Span 8 Pod is capable of accessing all types of labware supported by the Biomek NX instrument however specific types of tips are recommended to access specific types of labware refer to CHAPTER 1 Labware and Tip Compatibility with the Span 8 Pod 2 4 987887AE Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod To configure probes the right pane Figure 2 2 Figure 2 2 Hardware Setup Showing the Configuration View for a Span 8 Pod Biomek Hardware Setup Reconnect ff Home All Axes gb Add Device s Rem Biomek NX SN None M Probe Configuration J Accept gt lt Cancel ings Restore Settings Delete Settings fa AccuFrame Click to select which probes to configure SIDE of ng ny 8 Rodi Last Validation Not Validated Set Validation Time amp Devices 47 DeviceControllerO Axis Limit Settings oF ShakerALPO x cm cm Z cm D ul Span cm oF ShakerALP1 T Minimum feat 5 5 1
16. Each time a Vector Builder button is pressed the Movement Vector is changed in the corresponding axis by the amount indicated in the Delta box Up and Down move the pod in the Z axis Left and Right move the pod in the X axis and Back and Fwd move the pod in the Y axis Widen Narrow change the Span of the probes Aspirate Dispense move the probes on the Span 8 Pod in the D axis based upon the selection made in D Units Viewing the Current Position of the Pod and Gripper 4 8 The current position of the Span 8 Pod is displayed as five coordinates The X Y Z and D axes coordinates are the distances from the home position and the Span is the distance between the probes Z and D coordinates are displayed for each probe since probes can move independently in these axes 987887AE Manually Controlling the Biomek NX in Biomek Software 4 Using Advanced Manual Control with Span 8 Pod and Gripper If a gripper is installed the current position of the gripper is displayed as four coordinates The Y and Z axes are the distances from the home position Spin is the angle of rotation of the gripper and Grip is how much the gripper fingers are squeezed X Y and Z are displayed in centimeters the D axis is displayed in microliters and Span is displayed in millimeters To view the current position of a pod 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Po
17. Once assembled these two pieces constitute the syringe To assemble and install syringes 1 Remove the syringe components plunger and glass casing from their original packaging material 2 Moisten the Teflon seal on the end of the plunger with water 987887AE Span 8 Pod 1 Pump Assembly 3 Insert the end of the plunger with a Teflon seal into the glass casing 4 pushthe plunger into the glass casing until it stops Figure 1 24 The syringe is now assembled Figure 1 24 Installing Syringes Assembling the Syringe ia 6 od 1 Glass casing 2 Teflon seal 3 Plunger 5 Gently insert the top of the syringe into the valve Figure 1 25 6 screw the top of the syringe into the valve by turning the syringe to the right until finger tight Figure 1 25 NOTE Make sure the syringe is secure to prevent air entering the system and fluid leaks around the syringe 987887AE 1 35 Span 8 Pod Pump Assembly Figure 1 25 Installing Syringes Push the Syringe Up into the Valve and Screw it in o r E OH AARO oO Pump Valve Top of the syringe Syringe Base of the syringe Thumbscrew Au RWN 7 Push the thumbscrew up to the base of the syringe Figure 1 26 1 36 987887AE Span 8 Pod 1 Pump Assembly 8 Tighten the thumbscrew at the base of the syringe by turning the thumbscrew to the right until finger tight Figure 1 26 Installing Syringes Push the Thumbsc
18. correlates to the 1 on the numeric keypad while Down is found on the 2 Left is found on the 4 Right on 6 Up on 8 and Back on 9 987887AE Framing Instructions 3 Manually Framing Deck Positions on the Biomek NX Instrument 3 Once the tip is just touching the target position select Finish The pod moves up to its maximum height in the Z axis Manual Teaching closes and Position Properties appears Figure 3 10 4 Choose OK to save the framing information and close Position Properties 5 Repeat the procedure to frame additional deck positions using manual teach 6 Choose Save to save framing information for all positions and close the Deck Editor Figure 3 9 NOTE Choosing Cancel loses all changes to the deck including framing information since the Deck Editor was opened After all positions have been framed remove the framing shaft from the Span 8 Pod Manually Framing Using the Gripper When probes cannot descend far enough to access wells in labware for pipetting purposes the labware positions may be framed manually for access by the gripper This allows the labware to be moved from position to position Manually framing using the gripper consists of picking up a piece of labware from a previously framed position and placing it on the desired position to frame Positions that are framed via this process are accessible by the gripper only and cannot be the source or destination of pipetting
19. follow the recovery instructions displayed on Find Sensitivities Load Tips Figure 2 15 Figure 2 14 Find Sensitivities Load Tips Error Find Sensitivities Start Load APS6_200uL_LLS gt tips onto mandrels M A ABA HH at Position P5 and unload them Y once testingis completed Load Tips Click on a deck pesition to select the bp load position Update Settings cee Lie Figure 2 15 Find Sensitivities Choose Position 1 P9 has been selected where the pod can determine its LLS 9 select a deck position by clicking on the position NOTE The deck position must be an ALP with a solid ground plane for example a 1x3 Passive ALP If using a 1x1 Passive ALP or High Density 4x3 Passive ALP the deck position must have an LLS plate installed 987887AE 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Configuring the Span 8 Pod 10 Choose Next Find Sensitivities Test Sensitivities appears Figure 2 16 A few seconds later testing begins and test progress is tracked Figure 2 17 Figure 2 16 Find Sensitivities Test Sensitivities Find Sensitivities 5 atat Testing Progress Load Tips Initializing device for sensitivity tests Choose Position Test Sensitivities Update Settings Cancel a Figure 2 17 Find Sensitivities Test Sensitivities Indicating Testing Progress Find Sensitivities Start z a Testing Progress Load Tips Determining maximum
20. 2 Home All Axes 2 2 manually controlling 4 2 moving to a specific deck position 4 4 stopping in Manual Control 4 5 pod movements for Span 8 Pod 1 3 pod settings deleting 2 28 restoring 2 27 saving 2 26 probes configuring in Hardware Setup 2 4 Index defined 1 4 identifying 1 thru 8 3 4 overview 1 2 pump assembly defined 1 32 overview 1 2 pumps defined 1 32 purge pump configuring 2 7 defined 1 52 R Reconnect using in Hardware Setup 2 2 Remove Device using in Hardware Setup 2 3 S safety notice chemical and biological safety 0 viii cleaning 0 x electrical safety 0 vii instrument safety precautions 0 vi maintenance 0 x septa fluted tips 1 4 1 6 Span 8 Pod current position displayed as coordinates 4 8 defined 1 1 disposable tips 1 22 fixed tips 1 14 labware and tip compatibility 1 8 main components of 1 2 probes 1 2 pump assembly 1 2 supply container 1 41 system fluid 1 41 tubing 1 41 waste container 1 41 movement values defined 4 12 4 13 movements defined 1 3 moving to a safe roving height 4 9 overview 1 1 performing absolute moves 4 13 performing relative moves 4 10 preventative maintenance 1 54 pump assembly defined 1 32 Index 3 Index Index 4 purging air from the syringes and tubing 4 17 setting valve states 4 15 syringes 1 33 tips supported 1 4 troubleshooting 1 54 using non supported system fluids 1 41 wrist ground str
21. 2 Input 6 Pump 3 Valve 7 Syringe 4 Thumbscrew Syringes A syringe is housed in each of the pumps in the pump assembly Figure 1 23 Syringes provide pipetting accuracy by controlling the amount of system fluid aspirated from and dispensed into the tubing for each of the probes The volume of fluid aspirated and dispensed by the syringes is equivalent to the volume the probes aspirate and dispense How Syringes Affect Pipetting Accuracy Syringes affect pipetting accuracy by Controlling the amount of system fluid aspirated from and dispensed into the tubing for each of the probes NOTE The volume of fluid aspirated into and dispensed from the tubing is approximately equivalent to the volume of fluid a tip aspirates and dispenses e Maintaining a minimum and maximum operational speed which dictates how fast fluid can be aspirated into and dispensed from the syringes and ultimately the tips Controlling the amount of fluid dispensed per second by a syringe before the dispense action of the syringe abruptly stops NOTE The speed at which fluid is dispensed prior to the dispense action abruptly stopping is called the Cutoff Velocity If the Cutoff Velocity in the technique or liquid type governing the pipetting operation is too slow droplets can be left at the end of tips after a dispense pipetting operation rather than all the liquid dispensing into the labware 987887AE 1 33 Span 8 Pod Pump Assembly 1 34 Syringe Si
22. 8 probe Figure 1 8 987887AE 1 15 Span 8 Pod Interchangeable Tips 1 16 2 while holding the tip in one hand push the tubing down over the tapered end of the tip until it is flush with the flat portion of the tip with the other hand Make sure the tip is secure to prevent air entering the system and fluid leaks around the tip NOTE The ends of the tubing may become damaged or stretched after repeated tip installation and removal It may be necessary to cut a small portion 1 2 in of tubing off the end prior to attaching to ensure a tight fit NOTE Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping while pushing the tubing down onto the tip Figure 1 8 Inserting a fixed tip into the tubing Probe Tip Interface Tubing Fixed Tip PWN 3 Gently push the tubing and tip up into the Span 8 probe 987887AE Span 8 Pod 1 Interchangeable Tips 4 Pass the collar up over the tip to the tip interface Figure 1 9 Figure 1 9 Passing Collar Over Fixed Tip Probe Tip Interface Fixed Tip Collar PWN 5 screw the collar clockwise onto the tip interface until a tight fit is achieved The tip is now installed Figure 1 10 987887AE Span 8 Pod Interchangeable Tips 1 18 6 Repeat steps 1 through 5 for any other probes requiring fixed tips Figure 1 10 Installed Fixed Tip PWN Probe Tip Interface
23. 987887AE Purge Syringes flush system fluid through the syringes and tubing to clear air bubbles Purge System flush system fluid through the purge pump and tubing to clear air bubbles To purge air from the syringes and tubing 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the pod appears Figure 4 7 4 Choose Purge Syringes to flush system fluid through the tubing and syringes to clear air bubbles OR Choose Purge System to flush system fluid through the tubing and the purge pump to clear air bubbles The following Warning appears Figure 4 12 Figure 4 12 Warning that Probes are Moving to the Washstation Warning ee x The probes are about to go down to the washstation Probes 1 4 have no tips Probes 5 8 have no tips Press OK to continue or Cancel to abort Cancel l NOTE A different warning appears if a Span 8 Tip Wash Station is not defined and installed on the deck Take the appropriate action to address the warning and choose OK to continue purging the tubing 4 17 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper 5 Choose OK to continue the purge Information appears Figure 4 13 Figure 4 13 Information information xl i When the intake is clear of bubbles press OK 6 Follow the instructions d
24. Bar Code Reader Alignment Bracket Adjustment Screws Bar Code Cable Lamp Bar Code Reader Mount M4 x 6 Flat 1 2 3 4 5 B 14 987887AE Using a Stacker Carousel B Integrating the Bar Code Reader Attaching Bar Code Reader to Shuttle Attach the bar code reader to the Stacker Carousel shuttle as follows 1 Remove the mount access cover Figure B 12 and store in a safe place Figure B 12 Bar Code Reader Shuttle Mount Access Cover Removed 1 Shuttle Mount Access Cover 2 Bar Code Reader Shuttle Mount 2 Screw mount standoffs onto shuttle arm assembly mount points inside shuttle mount access Figure B 13 Figure B 13 Mounting Bracket Attachment 1 Fasteners 2 Mount Base 3 Mount Standoffs 987887AE B 15 Using a Stacker Carousel Integrating the Bar Code Reader 3 Using the fasteners attach mount base to standoffs 4 Attach bar code reader bracket assembly to bar code reader mount base Figure B 14 Figure B 14 Mounting Bar Code Reader 1 Bar Code Reader Bracket Assembly 2 Bar Code Reader Mount Base 3 Bar Code Reader Laser Beam Positioning Bar Code Reader The Stacker Carousel bar code reader can be mounted to scan any one of the four sides of a microplate NOTE For maximum scanning reliability it is recommended that labels be applied to the narrow sides of the microplate This label orientation accommodates scanning of skewed labels and requires less adjustm
25. Choose Start gt Programs gt Beckman Coulter gt Biomek Software Biomek Software appears 2 From the Instrument menu choose Hardware Setup Hardware Setup appears B 26 987887AE Using a Stacker Carousel B Configuring the Stacker Carousel in Hardware Setup 3 Choose Add Device gt Stacker Carousel New Devices appears Figure B 29 Figure B 29 New Devices E Available Devices Dy lest Re 00 Drainable Retillable Reservoir HW Address 01 MagBead amp LP HW Address 00 MagBeadA amp LP HW Address 01 MagBeadA amp LP HW Address 02 MagBead4LP Hw Address 03 L OrbitalShaker4LP Hw Address 00 OrbitalShaker amp LP Hw Address 01 OrbitalShakerALP HW Address 02 OrbitalShakerALP HW Address 03 PositivePositionSLP HW Address 00 PositivePositionSLP HW Address 01 Purge Pump HW Address 01 Simple Source Waste Sensor HW Address 01 Stacker Carousel Tiploader HW Address 2 Tiploader Hw Address 3 WashPump Install Cancel 4 Check Stacker Carousel and choose Install The device is displayed under Stacker Carousels in the left pane NOTE The first Stacker Carousel added to the instrument is named Stacker1 the second is named Stacker2 and so forth OR Right click Stacker Carousel and choose Add Device gt Stacker Carousel Figure B 29 The device is displayed unde
26. NX Figure B 9 Front View of Stacker Integration Adela Using a Stacker Carousel Integrating the Stacker Carousel B Using a Stacker Carousel Integrating the Stacker Carousel 5 Connect the Stacker Carousel Figure B 10 NOTE To connect the Stacker Carousel connect the supplied serial cable from the side panel of the Stacker Carousel to a PC Controller s communication port If more than two communication ports are required install the extra serial ports in the PC at this time Figure B 10 Stacker Carousel Final Placement Top View Rear Position Right Side omma Operating the Stacker Carousel To operate the Stacker Carousel refer to the Stacker Carousel User s Manual PN 148598 supplied with the Stacker Carousel NOTE While it is possible to pipette liquid to and from a 96 well plate on the Stacker Carousel shuttle it is not possible to pipette liquid to and from a 386 well plate that is sitting on the Stacker Carousel shuttle NOTE The Stacker Carousel shuttle must be framed using Teach do not use Auto Teach or Manual Teach Refer to the Stacker Carousel User s Manual for more information
27. Quick Disconnect insert Polypropylene EPDM and Stainless Steel Supply tubing Tygon 2075 Manifold Inlet fitting Polypropylene Manifold There are two styles of manifolds Teflon manifolds Part 717892 Polypropylene manifolds Part 719964 Tubing manifold to pump Teflon Stainless Steel and Polypropylene fittings should not contact liquid if tightened properly Pump valve Kel F Valve plug Virgin Teflon Syringe Glass Syringe piston seal Teflon Tubing pump to the tips Teflon NOTE Polypropylene fittings should not contact liquid if tightened properly Tips Fixed Disposable Conductive Disposable tips Stainless Steel or Teflon Coated Stainless Steel Polypropylene Polypropylene Impregnated with Carbon Disposable tip mandrel Stainless Steel NOTE All fitting threads may be wrapped with Teflon tape 987887AE Parts Along the Wash Station System Fluid Flow Path Since system fluid may be used as a wash fluid each of the Span 8 Tip Wash ALP parts that comes into contact with the system fluid or waste fluid must also be considered for possible susceptibility to the caustic nature of non supported system fluid and is therefore listed in Table 1 6 Table 1 6 Parts and Materials Along the Span 8 Tip Wash ALP Flow Path Part Material 8 Channel Passive Wash ALP Polypropylene Outlet Fitting Polypropylene Tubing Tygon 2075 Quick Disconnect insert Po
28. Shift deck shifts all ALPs and positions resulting in incorrect coordinates if applied to previously framed ALPs and positions Shift deck shifts all ALPs and positions associated with the deck by the change amounts shown Choose Shift deck when framing the first location of a new deck The shift amount required typically is not large but everything on the deck may need to move 1 cm for example Shift ALP shifts the entire ALP and all deck positions associated with the ALP by the change amounts shown Shift ALP typically is precise enough for using 96 well plates Shift position shifts only the deck position containing the AccuFrame by the amounts shown Shift position is the most precise teaching procedure and it is useful when 384 well microplates are used especially on larger ALPs such as the 4x4 otherwise Shift ALP is usually sufficient NOTE When framing a multiple position ALP 1x3 1x5 4x3 or 4x4 Shift ALP on the first position then Shift position on the rest 3 10 987887AE Framing Instructions 3 Manually Framing Deck Positions on the Biomek NX Instrument Manually Framing Deck Positions on the Biomek NX Instrument Biomek NX Manual Framing Wizard is a wizard type interface that is used to manually frame deck positions primarily for using high density labware Since the wells of high density labware are relatively small using the Manual Framing Wizard helps to ensure that the tips can access the wells
29. Side Scanning Position B 18 Bar Code Reader in Wide Right Side Scanning Position Bottom View B 18 Bar Code Reader in Narrow Side Scanning Position B 19 xxi Illustrations xxii B 20 BiZt B 22 B 23 B 24 B 25 B 26 B 27 B 28 5 29 B 30 B 31 Bar Code Reader in Rear Narrow Side Scanning Position Bottom View B 19 Bar Code Reader in Wide Left Side Scanning Position B 20 Bar Code Reader in Wide Left Side Scanning Position Bottom View B 20 Bar Code Reader in Front Narrow Side Scanning Position B 21 Bar Code Reader in Front Narrow Side Scanning Position Bot tom View B 21 Bar Code Reader Attachment Bracket B 22 Bar Code Reader Laser Beam Position Over Bar Code Label B 24 Adjust Laser Alignment as Necessary B 25 Label Positioning on Microplate B 26 New Devices B 27 Installing a Stacker Carousel by Right Clicking B 27 Hardware Setup Showing the Configuration View for a Stacker Carousel B 28 Adding the AccuFrame and Framing Tool Adaptor to the Stacker Carousel Shuttle B 30 Tables Ll 1 2 1 3 1 4 15 1 6 1 7 Zul Zed 23 2 4 dak 4 1 Al Tables Span 8 Pod Axes Movement 1 3 Span 8 Tip Types 1 7 Labware and Tip Compatibility 1 8 Span 8 Cutoff Velocities for Syringes 1 40 Parts and Materials Along the Span 8 Fluid Flow Path 1 42 Parts and Materials Along the Span 8 Tip Wash ALP Flow Path 1 43 Span 8 Pod Troubleshooting 1 54 Hardware Setup Opti
30. Span 8 Pod Troubleshooting If Then Power is lost to the pod Contact a Beckman Coulter Representative Motion is lost in an axis Contact a Beckman Coulter Representative The probes do not work properly Contact a Beckman Coulter Representative 1 54 987887AE 987887AE Span 8 Pod Troubleshooting Table 1 7 Span 8 Pod Troubleshooting If Then the Span 8 Pod is leaking from tubing connections Cut off approximately 1 2 in of tubing to remove the damaged portion before reattaching the tubing Leaking is occurring around syringes Tighten the syringes Leaks are occurring around fixed tips Make sure the tip is inserted securely into the tubing Cut off approximately 1 2 in of tubing off the end to ensure a tight fit Make sure the collar is securely tightened to the tip interface Leaks are occurring around disposable tips Make sure the disposable tip mandrel is inserted securely into the tubing Cut off approximately 1 2 in of tubing off the end to ensure a tight fit Make sure the collar is securely tightened to the tip interface Disposable tips are not mounting correctly Make sure the tip shuck tube is securely tightened to the tip interface Disposable tips are not shucking Make sure the collar is securely tightened to the tip interface If the collar is loose turn the collar clockwise until a tight fit is achieved Refer to Installin
31. The available configurations for a Span 8 Pod are Span 8 with gripper tool NOTE The gripper tool is factory installed and may not be installed later Span 8 without gripper tool NOTE Refer to CHAPTER 1 Span 8 Pod for detailed information on the Span 8 Pod probes and gripper Control Modes The Biomek NX Span is controlled using Biomek Software operated from the host computer The Biomek NX can be Operated as a standalone instrument gripping and moving microplates without the assistance of a laboratory robot if the gripper is installed XXV Introduction System Components Integrated into an automated robotic system Operated using a combination of these capabilities System Components Towers xxvi The system components described below correspond to the configurations in Figure 1 Figure 1 Biomek NX with Span 8 and Optional Gripper 1 Tower 4 Span 8 Pod 2 Gripper 5 Deck 3 Tower The Biomek NX Span 8 towers Figure 1 form the two upright sections of the base unit along which the pod travels in the X axis The links for master control of the Biomek NX system plus utility hook ups and ALP connections are on the towers A status indicator panel with green and amber indicator lights is built into the main cover and keep users aware of the current operational status of the Biomek NX refer to Indicator Lights The towers house the following connections Main power switch on the outsi
32. Using Non Supported System Fluids System fluids other than those listed as supported system fluids refer to System Fluid may damage the components along the Span 8 system fluid flow path and the Span 8 Tip Wash ALP Therefore before using non supported system fluids use Table 1 5 to verify that component parts along the Span 8 system fluid flow path are not susceptible to the caustic nature of any non supported system fluids Use Table 1 6 to verify the parts on the Span 8 Tip Wash ALP are not susceptible to the caustic nature of any non supported system fluids NOTE All system fluid whether supported or non supported must be degassed prior to use The system must then be primed with the new system fluid and the Span 8 system tubing must be purged of air Refer to Preparing the Liquid System for more information on degassing priming and air purging procedures 987887AE 1 41 Span 8 Pod Liquid System 1 42 Parts Along the Span 8 System Fluid flow Path Each of the Span 8 system parts listed in Table 1 5 comes into contact with the system fluid and must be considered for possible susceptibility to the caustic nature of non supported system fluid Table 1 5 Parts and Materials Along the Span 8 Fluid Flow Path Part Material Carboy Polypropylene Supply carboy tubing Tygon 2075 Carboy Cap Polypropylene Tubing cap to quick disconnect Tygon 2075 Quick Disconnect Body Polypropylene EPDM and Stainless Steel
33. Well Comer Well Center Go Finish cont OR 3 16 987887AE 987887AE Framing Instructions 3 Manually Framing Deck Positions on the Biomek NX Instrument Select Well Center to align the tips to the center of the wells Figure 3 16 Framing to the Center of a Well W Biomek NX Manual Framing Wizard lol x The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Center and press Go Press Next gt when the tips are directly above their target Warning Technique a Back Left Bight f A S 4 Ewd Down JV Hysteresis compensation Delta 0 05 tom Total Moved From Start cm Teach X Y 0 0 0 Reset C well Comer Well Center Go Finish To align the tips in the X and Y axes with the wells of the microplate on top of the ALP lower the tips in the Z axis until they are approximately 1 mm above the top of the ALP cont NOTE Since tip height is set in the next step in the Manual Teaching process it is safe to move the pod to any height to make aligning the tips with the microplate easier Visually verify the physical position of the tips in relation to the physical position of the wells of the microplate on top of the ALP Adjust the position of the tip to align it with the microplate well or corner by either Using the Graphic Alignment Tool e Using the Delta Value
34. a Beckman Coulter Representative Hardware Setup is used for Configuring the Biomek NX Span 8 Instrument Configuring the Span 8 Pod Saving Restoring and Deleting Settings NOTE Any active ALPs that require a CAN communication or other devices integrated on the deck of the Biomek NX must also be configured in Hardware Setup Refer to the ALPs User s Manual or the specific device integration manual for instructions on configuring a specific ALP or device in Hardware Setup Accessing Hardware Setup Hardware Setup Figure 2 1 is accessed from within the Biomek Software To access Hardware Setup 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software Biomek Software appears 987887AE 2 1 Configuring the Biomek NX Span 8 in Biomek Software Overview 2 From the Instrument menu choose Hardware Setup Hardware Setup appears Figure 2 1 Figure 2 1 Hardware Setup for a Biomek NX Span 8 Instrument Biomek Hardware Setup J Bl Reconnect A Home All Axes Add Device S Remove Device J Accept gt lt Cancel Serial Number fa AccuFrame 8 Podi Port Simulate gt Devices 67 DeviceControllerO Pod Type Span Pod v oF ShakerALPO 7 ShakerALP1 g7 StirrerALPO oF StirrerALP1 amp Digital Devices B E Simulator E Camera OD Materials Stacker Carousels BiomekNX Span Understanding the Options in Hardware Setup An understanding of the options on the toolbar in Har
35. and Directional Buttons 3 17 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument Using the Graphic Alignment Tool The graphic alignment tool Figure 3 17 is used to instruct Biomek Software of the current position of the tips to the physical position of the wells of the microplate on top of the ALP Biomek Software uses the information it is given to move the pod so the tips are directly above the target position Figure 3 17 Manual Teaching the X and Y Axes Ml Biomek NX Manual Framing Wizard 10 x The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Center and press Go Press Next gt when the tips are directly above their target Warning Delta 0 05 Hem a Back Left Bight 5 Ewd Dom JV Hysteresis compensation Technique Total Moved From Start cm Teach XY 0 0 0 Reset C Well Comer Well Center Go 1 Graphic Alignment Tool The graphic alignment tool is a visual representation of the tip small circle and the target position on the labware positioner The small circle is moved until it represents the tip s current physical location in relation to the target position on the labware positioner To use the graphic alignment tool 1 Drag the center small circle until it represents the tip s physical position in relation to the physical
36. and negative values for the gripper are as follows Figure 4 9 Vector Builder for the Span 8 Gripper 7 s gt 4 gt Squeeze Unsqueeze era ha Rotate Left Rotate Right Fwd Positive value Y forward Back Negative value Y back motion of the gripper motion of the gripper Up Positive value Z up motionof Down Negative value Z down the gripper motion of the gripper e Rotate Left Positive Spin left e Rotate Right Negative Spin right rotation of the gripper rotation of the gripper e Squeeze Positive Grip e Unsqueeze Negative Grip unsqueezing motion of the gripper squeezing motion of the gripper Performing Absolute Moves for the Pod and Gripper Absolute Move allows the pod and gripper to move to a specific coordinate position in the workspace of the instrument Use Absolute Move when the coordinates of the desired position are known When an Absolute Move is entered the values displayed in Movement Vector reflect the relative move required to physically move the pod to the desired position NOTE Make sure the physical location of the pod is not changed between the time the vector is built and the time the Go button is pressed 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the selected Span 8 Pod appears Figure 4 7 987887AE 4 13 Manually Controlling the Biome
37. attached to the tip interface of the probes These tips may or may not be conductive refer to Interchangeable Tips NOTE The Span 8 Pod can perform liquid level sensing LLS or clot detection during any liquid transfers but only if conductive tips fixed or disposable are attached to the probes Interchangeable Tips Use Manual Control to move the probes in the Y axis Dragging the probes by hand may affect alignment and the ability to access labware Use care when handling septa fluted tips they are extremely sharp Use care when handling septa fluted tips they are extremely fragile Interchangeable tips are attached to the probes to perform liquid handling procedures Three styles of interchangeable tips are used on the Span 8 Pod Fixed Disposable Septa Fluted fixed but also capable of piercing septum when the Requires Piercing Tips property of the Septum Piercing Tube Racks is checked in the Labware Type Editor refer to the Biomek Software User s Manual PN 987835 Editing Labware Type Properties 987887AE Span 8 Pod Interchangeable Tips Liquid Level Sensing 987887AE If the liquid level in a piece of labware is unknown the Span 8 Pod can determine the liquid level when Liquid Level Sensing LLS capable tips are attached to the probes NOTE LLS tips must be attached to the probes to detect the liquid level NOTE An LLS plate option used on a 1x1 Passive ALP can improve LLS capabilities refer to
38. choose OK Figure 3 25 Figure 3 25 Warning to Make Sure Gripper is Ready for Framing xl The grippers cannot be holding anything for this operation Also make sure there is nothing between the pod and the location that you will frame the grippers at such as a stack of labware In Pick Position Figure 3 26 select a previously framed deck position and frame the gripper to that location The deck position must be framed first and should be a small passive ALP if possible NOTE Make sure that the AccuFrame is correctly placed on the selected position refer to Installing AccuFrame Figure 3 26 Pick Previously Framed Deck Position to Frame the Gripper Pick Position x Place the framing tool on a position that has been framed select that position below and hit Ok i Cancel 987887AE Framing Instructions 3 Framing the Span 8 Gripper 8 Choose OK This moves the gripper to that position Manual Control Figure 3 27 and Frame gripper Figure 3 28 appear side by side NOTE The gripper is a little high and opened a little wide at the end of the move initiated by step 8 Figure 3 27 Manual Control for Moving Gripper During Framing Manual Control ft Home Z X Move 2 Max Move Gripper Z Max Purge Syringes Set Valve States Get Tip Status Purge System Absolute Move auto Clear G Clear E Refresh vector Builder X cm cm Span mm cm Z cm Spin Grip cm Back
39. covers to access electrical wiring or fuses Change only the fuses that are accessed from the outside of the instrument without removing covers Contact a Beckman Coulter Representative if further access is required Turn off and unplug power to the instrument before changing fuses Failure to do so can cause electrical shock or equipment damage The Biomek NX instrument uses any AC power source between 100V and 240V and holds two fuses Fuses are located in the outside right tower Figure 6 If power is lost check the fuses that are accessed from the outside of the instrument If it is suspected that the fuses are burned out change them by completing the following 1 Turn off the instrument power switch 2 Unplug the instrument from the power source 987887AE xxxiii Introduction Troubleshooting 3 Using a small flathead screwdriver pry open the fuse carrier containing two fuses Figure 6 from the AC power input module Figure 6 Replacing Fuses in Tower 4 Gently pull old fuses from the carrier by hand 5 Gently place new fuses into carrier by hand NOTE Use 250v 3 15 amp 5x20 Slow Blow type fuses for replacement 6 Replace the fuse carrier into the AC power input module 7 Plug the instrument into the power source Turn on the instrument power switch XXXIV 987887AE CHAPTER 1 Span 8 Pod _ Overview The Span 8 Pod is a self contained working unit installed o
40. decontaminate components of the instrument before requesting service by a Beckman Coulter Representative or returning parts to Beckman Coulter Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so If any parts are returned they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated xX 987887AE Safety Notice RoHS Notice RoHS Notice These labels and materials declaration table the Table of Hazardous Substance s Name and Concentration are to meet People s Republic of China Electronic Industry Standard S J T11364 2006 Marking for Control of Pollution Caused by Electronic Information Products requirements China RoHS Caution Label This logo indicates that this electronic information product contains certain toxic or hazardous substances or elements and can be used safely during its environmental protection use period The number in the middle of the logo indicates the environmental protection use period for the product The outer circle indicates that the product can be recycled The logo also signifies that the product should be recycled immediately after its environmental protection use period has expired The date on the label indicates the date of manufacture g lt S 2 S S g lt 38 A H Mfg Date China RoHS Environmental Label This logo indicates that the product does not contain any toxic or h
41. e Using the Graphic Alignment Tool e Using the Delta Value and Directional Buttons Adjust the Z position height of the gripper to position the microplate appropriately in the ALP using the Up and Down directional buttons and delta value refer to Using the Delta Value and Directional Buttons Using the Graphic Alignment Tool The graphic alignment tool Figure 3 22 is used to instruct Biomek Software of the current position of the gripper in relation to the position being framed Biomek Software uses the information it is given to move the pod so the gripper is directly above the target position 3 25 3 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument Figure 3 22 Manual Teaching the X and Y Axes 15 x The labware should be 2 cm above the position Lower the labware into the correct position Press Next gt when the labware is correctly seated in the position E Warming 5 a an Delta 10 05 cm E Technique y X Back Left 4 gt Right gt 0 Ad Ewd Om Ean mm 0 0 0 5 Total Moved From Start cm Finish O 1 Graphic Alignment Tool The graphic alignment tool is a visual representation of the back left corner of the microplate small circle and the position being framed The small circle is moved until it represents the microplate s current physical location in relation to the position being framed To use the graphic alignment
42. fo f3 Maximum 102 79 fo fiz 1381 fiooo fia7 Set X Set Additional Pod Settings Y Gripper Settings Correlate Pods Correlate 2 Axes Find LLS Senstivities Pump PurgePumpo x Find CD Sensitivities BiomekNX Span 987887AE 2 17 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod 2 18 3 Figure 2 20 Find Clot Detection Sensitivities Start Find Clot Dete Start This wizard assists with determining the maximum sensitivity for each probe This will help minimize errrors created by receiving a false Load Tips detection All of the probes will need to have tips loaded Any probe configured to use disposable tips will have to be loaded with a conductive tip The currenttip confiquration is Probe 1 Disposable tip P200_LLS No tip detected Probe 2 Disposable tip P200_LLS No tip detected Probe 3 Disposable tip P200_LLS No tip detected Probe 4 Disposable tip P200_LLS No tip detected Probe 5 Disposable tip P200_LLS No tip detected Probe 6 Disposable tip P200_LLS No tip detected Probe 7 Disposable tip P200_LLS No tip detected Probe 8 Disposable tip P200_LLS No tip detected Load Tips will not be displayed on the left side if all probes are configured with fixed tips NOTE On the left side of Find Clot Detection Sensitivities a list of steps required to complete the procedure is displayed As the
43. on the Span 8 Pod NOTE A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tip mandrels on the Span 8 Pod refer to Electro Static Discharge Protection when Handling Tips on the Span 8 Pod To install disposable tip mandrels 1 Place the end of a disposable tip mandrel into the tubing that extends from the bottom of the tip interface Figure 1 14 Figure 1 14 Inserting a Disposable Tip Mandrel into the Tubing 1 Probe 3 Tubing 2 Tip Interface 4 Disposable tip mandrel 987887AE 1 23 1 Span 8 Pod Interchangeable Tips 2 while holding the disposable tip mandrel in one hand push the tubing down onto the disposable tip mandrel with the other hand Make sure the disposable tip mandrel is secure to prevent air entering the system and fluid leaks around the tip NOTE The ends of the tubing may become damaged or stretched after repeated tip installation and removal It may be necessary to cut a small portion 1 2 of tubing off the end prior to attaching to ensure a tight fit NOTE Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping while pushing the tubing down onto the tip 3 Gently push the tubing and disposable tip mandrel up into the tip interface 4 Pass the collar up over the disposable tip mandrel to the tip interface Figure 1 15 Figure 1 15 Passing Collar Over a Disposable Tip Mandrel
44. operations 987887AE 3 23 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument Figure 3 20 Manual Framing Wizard Setup for Framing with Gripper E Biomek NX Manual Framing Wizard a oj xj Load A gt plate from position must be framed Technique E ae Finish To manually frame positions using the gripper 1 1n Load a select the type of labware to move to the desired position to frame 2 Infrom position select a previously framed deck position for the starting position of the labware to move 3 24 987887AE 987887AE Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument 3 Choose Next The gripper picks up the labware from the framed position and move it approximately 2 cm above the desired position to frame Manual Framing Wizard Teach X Y appears Figure 3 21 Figure 3 21 Manual Framing Wizard Teach X Y with Gripper zox The labware should be 2 cm above the position Lower the labware into the correct position Press Next gt when the labware is corectly seated in the position Warning RE Delta 0 05 cm Technique a Back Left Bight 0 9 s Ewd Don 5 Total Moved From Start cm Teach X Y mm jf jo fo Finish 4 Adjust the X and Y positions of the gripper to position the microplate appropriately in the ALP by either
45. sensitivities for each probe Choose Position Test Sensitivities o Update Settings 2 15 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Do not choose Cancel if testing is stopped before the probe sensitivities have been completed liquid level sensing will not function and Find Sensitivities must be repeated 11 When testing is completed choose Next Find Sensitivities Update Settings appears indicating the probe sensitivities have been completed Figure 2 18 NOTE If tips have been configured to unload they will unload now Figure 2 18 Find Sensitivities Update Settings Start Updating Probe Sensitivities Completed E Load Tips Probe 1 3750 Updated Probe 2 3830 Updated tes es Probe 3 3860 Updated Probe 4 3900 Updated Update Settings Probe 5 3900 Updated Probe 6 3650 Updated Probe 7 3470 Updated Probe 8 3650 Updated 12 Choose Finish Find Sensitivities closes 13 Choose Accept Hardware Setup closes N Performing Clot Detection Sensitivities Performing Find Clot Detection Sensitivities is necessary to test each probe on an individual system to ensure the clot detection sensing capability is calibrated This procedure adjusts the system to the individual clot detection sensitivity settings on each probe Although Find Clot Detection Sensitivities is performed during initial setup by a Beckman Coulte
46. steps of Find CD Sensitivities are accessed they are highlighted on the left NOTE ASpan 8 Pod that is configured with all fixed tips will not display Load Tips on the left side of Find Clot Detection Sensitivities Choose Next Find Clot Detection Sensitivities Load Tips appears Figure 2 21 NOTE If tips that were not configured in Hardware Setup are detected or if there are probes that must be calibrated Find Clot Detection Sensitivities Tip Configuration appears If probes are not calibrated configure the disposable tip in Find Clot Detection Sensitivities Tip Calibration to match the tip that is currently attached to the probe Choose Next to proceed through the wizard 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Configuring the Span 8 Pod Figure 2 21 Find Clot Detection Sensitivities Load Tips Start Load AP96_200uL_LLS tips onto mandrels 6 E Load Tips at Position P5 and unload them v once testing is completed Click on a deck position to select the tip load position a g Orbitall Update Settings NOTE If all probes are configured with fixed tips Find Clot Detection Sensitivities Choose Position appears rather than Find Clot Detection Sensitivities Load Tips Go immediately to step 9 4 InLoad choose the appropriate tip type NOTE Any probe configured to use disposable tips must be loaded with a conductive tip 5 in mandrels choose the desired probes 6 click on th
47. system configuration 1 In Biomek Software select Instrument gt Hardware Setup to open Hardware Setup Hardware Setup appears Figure 2 5 Figure 2 5 Hardware Setup A Reconnect ft Home All Axes db Add Device s Remo ice Vv Accept gt lt Cancel EE Siomek Nx SN None Serial Number lr a AccuFrame 8 Podi Port Simulate z amp Devices F DeviceControllerO Pod Type Span Pod oF ShakerALPo oF ShakerALP1 oF StirrerALPO oF StirrerALP1 Digital Devices o Simulator E Camera OD Materials Stacker Carousels BiomekNX Span 1 List of Devices 2 Select dh Add Device New Devices appears 3 Select Purge Pump HW Address 00 to add a purge pump the Biomek NX Span 8 instrument 987887AE 2 7 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod 4 In New Devices choose to add the new purge pump to the Hardware Setup and close New Devices A purge pump has been added to the Hardware Setup Figure 2 6 Go to step 4 below to associate the purge pump with the Span 8 Pod Figure 2 6 A Purge Pump Added to the Hardware Setup Biomek Hardware Setup 2 Reconnect ff Home all Axes dp Add Device Remove Device J Accept gt lt Cancel Biomek NX SN None ea AccuFrame 8 Podi a Devices F DeviceControllerO a oF ShakerALPO oF ShakerALP1 oF StirrerALPO oF StirrerALP1 amp Digital Devices FB Simulator E
48. the ALPs User s Manual PN 987836 1x1 Passive ALP with LLS Plate Option LLS tips are used to determine the liquid level in a piece of labware by detecting a shift in the capacitance The LLS tip moves to a specified height within a well and then slowly moves down into the well When the tip contacts liquid there is a large change in the capacitance detected The liquid level is sensed by determining the height at which this change in capacitance occurs CHAPTER 4 Verifying Liquid Level Sensing for more information on detecting the liquid level in labware Liquid Level Sensing Tips Biomek NX Span 8 supports the following liquid level sensing tips Fixed 60 Coated and Uncoated maximum of 5000 uL liquid and or air capacity Fixed 100 Coated and Uncoated maximum of 5000 uL liquid and or air capacity Septa Fluted maximum of 5000 pL liquid and or air capacity NOTE Fixed tips can pull samples into the tubing but the maximum sample volume is limited to syringe capacity refer to Syringes Disposable 20uL LLS up to 100 uL liquid capacity up to 120 pL air capacity Disposable 200uL LLS up to 220 uL liquid capacity up to 240 uL air capacity Disposable P1000 LLS sterile and non sterile up to 1050 uL liquid and air capacity Disposable P1000 LLS barrier up to 1025 uL liquid and air capacity NOTE Refer to nterchangeable Tips for more information on tips available for use with the Span 8 Pod Liq
49. tool 1 Drag the center small circle until it represents the physical position of the microplate in relation to the position being framed The small circle represents the back left corner of the microplate NOTE The software uses this graphical representation of the physical position of the microplate to know approximately how far in any direction the gripper must move 2 Select Go The gripper moves in accordance with the position of the small circle in relation to the large circle When the move is completed the small circle resets itself to the center of the large circle NOTE Select Go The gripper moves in accordance with the position of the small circle in relation to the large circle When the move is completed the small circle resets itself to the center of the large circle 3 26 987887AE 987887AE Framing Instructions 3 Manually Framing Deck Positions on the Biomek NX Instrument 3 Visually verify the position of the microplate in relation to the position being framed If the microplate is still not accurately positioned above the position being framed repeat steps 1 and 2 until the appropriate position is obtained NOTE The microplate may also be aligned with the position using the delta value and directional buttons refer to Using the Delta Value and Directional Buttons gt Once the microplate is aligned in the X and Y axes to the position being framed align the Z axis using the Up and Down directional
50. 2 Supply Tubing Bundle Lower oO 1 Lower Tubing Retainer 987887AE 1 47 Span 8 Pod Liquid System Waste Container 1 48 SPILL HAZARD Do not kink tubing between the waste container the Span 8 Tip Wash ALP and drip tray Observe cautionary procedures as defined by your safety officer when using toxic pathologic or radioactive materials Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer The waste container is an off deck disposal site for fluid from the Span 8 Tip Wash ALP refer to the ALPs User s Manual PN 987836 Span 8 Tip Wash ALP and drip tray The waste container attaches to the Biomek NX Span 8 instrument via a 3 8 in tubing line from the Span 8 Tip Wash ALP and a 1 4 in tubing line from the drip tray In the waste container the lines are bundled together using a tubing retainer a gasket and container cap NOTE Separate waste containers can be used for each Span 8 Tip Wash ALP on the deck if it is undesirable to mix the contaminates washed off the tips 987887AE Span 8 Pod 1 Liquid System Figure 1 33 Waste Container and Tubing i O i 1 Cap 2 Gasket 3 Tubing retainer Exchanging a Waste Container The waste tubing is initially bundled by a Beckman Coulter Representative when the Biomek NX Span 8 instrument is installed When waste containers are exchanged the bund
51. 28 Testing Gripper Framing Accuracy 3 34 Calibrating Gripper Rotation 3 34 Troubleshooting 3 37 Manually Controlling the Biomek NX in Biomek Software 4 1 Overview 4 1 Accessing Manual Control 4 1 Using Manual Control 4 2 Homing All Axes of the Span 8 Pod and Gripper 4 3 Moving a Pod to a Specific Deck Position 4 4 Stopping a Pod 4 5 Viewing the Firmware Version 4 5 Accessing Advanced Manual Control 4 5 Using Advanced Manual Control with Span 8 Pod and Gripper 4 6 Viewing the Current Position of the Pod and Gripper 4 8 Moving the Pod and Gripper to a Safe Roving Height 4 9 Performing Relative Moves for the Pod and Gripper 4 10 Setting Delta Values for the Pod and Gripper 4 11 Understanding and Using the Vector Builder 4 12 Performing Absolute Moves for the Pod and Gripper 4 13 Setting Valve States 4 15 Verifying Liquid Level Sensing 4 16 Purging Air from the Syringes and Tubing 4 17 Specifications A 1 Specifications A 1 Using a Stacker Carousel B 1 Overview B 1 Integrating the Stacker Carousel B 2 Positioning the Stacker Carousel on the Deck B 3 Mounting the Stacker Carousel B 5 Attaching the Alignment Bracket B 5 Attaching the Stacker Carousel B 7 Attaching the Side Shield B 8 Operating the Stacker Carousel B 12 Preventive Maintenance and Troubleshooting B 12 Integrating the Bar Code Reader B 13 Attaching Bar Code Reader Bracket B 13 Attaching Bar Code Reader to Shuttle B 15 Po
52. 3 14 Manual Framing Wizard if Tips are Loaded E Biomek NX Manual Framing Wizard Use currently loaded tips Warming Line tips up against PASKEW EEEE on position P4 Technique Teach x Y TeachZ Finish To manually frame deck positions using tips 1 Ifno tips are loaded in Load tips from the select the type of tips to load and the position on which the tip box is located Figure 3 13 2 InLine tips up against select the appropriate labware type of the labware placed in the position to frame 3 Choose Next to continue to the next screen Biomek NX Manual Framing Wizard Teach X Y appears 3 15 3 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument 4 Select Well Corner to align the tips to the corners or junction of four wells NOTE Well Corner is available only when framing to a deck position occupied by a piece of labware with square wells Figure 3 15 Framing to the Corner of Four Wells W Biomek NX Manual Framing Wizard loj x The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Comer and press Go Press Next gt when the tips are directly above their target Delta 0 05 fe cm Technique A a Back Left Bight Ewd Down JV Hysteresis compensation Setup Total Moved From Start cm Teach X Y 0 0 0 Reset
53. 5 938 0 fos oF StirrerALPO oF StirrerALP1 Maximum 102 79 40 fiz 1381 fiooo fis7 Digital Devices Probes 1 4 use sep eey E Simulator Fixed100 gt tips E Camera Materials x syringes Gorrelate Pods Find LLS Sensitivities amp Stacker Carousels Disabled Probes 1 2 3 4 5 6 7 8 Pump lt None gt z Find GO Sensitivities Y Additional Pod Settings Y Gripper Settings BiomekNX Span 2 To choose the probe type select the probes to configure from the graphical representation NOTE The graphical representation of the probes changes according to the type disposable or fixed From the drop down menu choose the tips in Probes 1 4 use tips NOTE To choose probes 1 through 8 at one time hold down Ctrl with probes 1 through 4 highlighted and then click on probes 5 though 8 NOTE Probes 1 through 4 may be configured with one type of fixed tips or disposable tips and probes 5 through 8 may be configured with another type of fixed tips or disposable tips To mix the type of tips select probes 1 through 4 and choose the tip type Then select probes 5 through 8 and choose the tip type NOTE When tips are changed from disposable to fixed or from fixed to disposable a Warning Figure 2 3 appears on Hardware Setup stating that the pod must be homed and Find Sensitivities performed If Accept is chosen another device is configured in Hardware Setup or a dialog is chosen from Tools another War
54. 6 B 17 B 18 B 19 Illustrations Hardware Setup 3 35 Warning to Make Sure Gripper is Ready for Framing 3 35 Pick Deck Position to Calibrate the Gripper 3 36 Align Gripper Rotation 3 36 Confirms Manual Control is Connecting 4 2 Manual Control 4 2 Warnings Must Be Addressed Before Homing Process Begins 4 3 Warning that the Span Probes are About to Go Down to the Washstation 4 4 Information for a Span 8 Pod 4 4 Firmware Version Information 4 5 Overview Advanced Manual Control for a Span 8 4 7 Vector Builder for the Span 8 Pod 4 12 Vector Builder for the Span 8 Gripper 4 13 Absolute Move for Span 8 Pod 4 14 Valve Settings 4 15 Warning that Probes are Moving to the Washstation 4 17 Information 4 18 Stacker Carousel B 2 Stacker Shuttle Positions B 3 Stacker Carousel Integration B 4 Attaching Alignment Bracket B 5 Attaching Riser Plate B 6 Stacker Attachment B 7 Attaching the Side Shield Components to Biomek NX B 9 Aligning the Stacker Carousel Shuttle B 10 Front View of Stacker Integration B 11 Stacker Carousel Final Placement Top View Rear Position Right Side B 12 Bar Code Reader Shuttle Mount Access Cover Removed B 14 Bar Code Reader Shuttle Mount Access Cover Removed B 15 Mounting Bracket Attachment B 15 Mounting Bar Code Reader B 16 Bar Code Reader in Narrow Side Scanning Position B 17 Bar Code Reader in Narrow Side Scanning Position B 17 Bar Code Reader in Wide Right
55. 7887AE Safety Notice Chemical and Biological Safety Before running with chemistry or any biological samples new labware types will require testing to determine if labware offsets are necessary to move to or from the Peltier ALP or to access the labware during pipetting operations while on the Peltier ALP If you do not do the required testing the labware could crash and the contents could spill if the offset is incorrect Normal operation of the instrument may involve the use of materials that are toxic flammable or otherwise biologically harmful When using such materials observe the following precautions Handle infectious samples according to good laboratory procedures and methods to prevent the spread of disease Observe all cautionary information printed on the original solutions containers prior to their use Dispose of all waste solutions according to your facility s waste disposal procedures Operate the instrument in accordance with the instructions outlined in this manual and take all the necessary precautions when using pathological toxic or radioactive materials Splashing of liquids may occur therefore take appropriate safety precautions such as using safety glasses and wearing protective clothing when working with potentially hazardous liquids Use an appropriately contained environment when using hazardous materials Observe the appropriate cautionary procedures as defined by your safety officer when u
56. B 2 NOTE Position P1 or P10 back mount or Position P2 or P11 front mount 987887AE B 5 Using a Stacker Carousel Integrating the Stacker Carousel 3 Attach the alignment bracket to the Biomek NX deck Figure B 4 4 Adjust the height of the alignment bracket so that the top of the bracket is flush with the bottom of the riser plate on which the Stacker Carousel is mounted then tighten the fasteners Figure B 5 5 place the riser plate over the alignment pins of the horizontal alignment bracket Figure B 5 6 Verify that the riser plate is set firmly on the Biomek NX alignment bracket and the table NOTE Ifthe Biomek NX has leveling feet attached place the 4 standoffs underneath each of the feet on the riser plate Figure B 5 Figure B 5 Attaching Riser Plate 1 Riser Plate 2 Standoffs 3 Riser Alignment Pins B 6 987887AE Using a Stacker Carousel B Integrating the Stacker Carousel Attaching the Stacker Carousel Attach the stacker as follows 1 Place the stacker onto the riser plate over the riser alignment pins Figure B 6 Figure B 6 Stacker Attachment 2 Level the stacker 987887AE B 7 Using a Stacker Carousel Integrating the Stacker Carousel 3 Adjust the height of the stacker until the distance between the Biomek NX deck surface and the top of the flat surface of the stacker shuttle measures 5 in 1 8 in NOTE If nece
57. CAO OF OOOO CAO OO OOO eae is gt asl a eal gt a GP al Pl a s ET e T E E E e E a RR A E ea SE a as M Eo a a N a OOOO cass real cas aa CP a A Figure B 20 Bar Code Reader in Rear Narrow Side Scanning Position Bottom View 1 Laser Beam 1 Laser Beam 987887AE Using a Stacker Carousel Integrating the Bar Code Reader B 20 Figure B 21 Bar Code Reader in Wide Left Side Scanning Position Ah ro 0 0 o fo p E ARN COLOK REKAL 0 0 0 0 0 0 0 0 ne 1 Laser Beam Figure B 22 Bar Code Reader in Wide Left Side Scanning Position Bottom View U 4 Fi E 1 Laser Beam 987887AE Using a Stacker Carousel B Integrating the Bar Code Reader Figure B 23 Bar Code Reader in Front Narrow Side Scanning Position 1 Laser Beam Figure B 24 Bar Code Reader in Front Narrow Side Scanning Position Bottom View 2 Al C oo 0 o 1 Laser Beam 987887AE B 21 Using a Stacker Carousel Integrating the Bar Code Reader Figure B 25 Bar Code Reader Attachment Bracket Bracket Fasten
58. Camera OD Materials Stacker Carousels PurgePump0 does not support any configuration options BiomekNX Span OR 1 From Hardware Setup right click on Devices 2 Select Add Device Figure 2 7 2 8 987887AE 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Configuring the Span 8 Pod 3 Select Purge Pump HW Address 00 to add a purge pump the Biomek NX Span 8 instrument Figure 2 7 Right Clicking Devices to Add a Purge Pump in Hardware Setup 2 Reconnect ff Home All Axes h Add Device s Remove Device Y Accept gt Cancel Biomek NX SN None AccuFrame 8 Podi 2 Devic D Pi SI Reconnect sl T R oF StirrerALPO oF StirrerALP1 amp Digital Devices FB Simulator OrbitalShakerALP HW Address 01 E Camera OrbitalShakerALP HW Address 02 OD Materials OrbitalShakerALP HW Address 03 PositivePositionALP HW Address 00 PositivePositionALP HW Address 01 MagBeadALP HW Address 00 MagBeadALP HW Address 01 MagBeadALP HW Address 02 MagBeadALP HW Address 03 OrbitalShakerALP HW Address 00 Stacker Carousels Purge Pump HW Address 01 Drainable Refillable Reservoir HW Address 00 Drainable Refillable Reservoir HW Address 01 Source Waste Sensor HW Address 00 Source Waste Sensor HW Address 01 BiomekNX Span Select the Span 8 Pod in
59. Collar Fixed Tip 987887AE Span 8 Pod 1 Interchangeable Tips Removing Fixed Tips Use care when handling septa fluted tips they are extremely sharp Use care when handling septa fluted tips they are extremely fragile SPILL HAZARD Use an appropriately contained environment when using hazardous materials Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer Observe cautionary procedures as defined by your safety officer when using toxic pathologic or radioactive materials Always wear protective gloves when removing the tips To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod NOTE A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tips on the Span 8 Pod refer to Electro Static Discharge Protection when Handling Tips on the Span 8 Pod 987887AE 1 19 Span 8 Pod Interchangeable Tips To remove fixed tips 1 Unscrew the collar from the bottom of the probe by turning the collar counterclockwise Figure 1 11 Figure 1 11 Fully Installed Fixed Tip Probe Tip Interface Collar Fixed Tip PWN 1 20 987887AE Span 8 Pod 1 Interchangeable Tips 2 Pull the collar down and off the tip Figure 1 12 Figure 1 12 Pulling the Collar off the Fixed Tip
60. Figure 1 6 987887AE 1 Span 8 Pod Interchangeable Tips Figure 1 3 Septa Piercing Tip Minimum Liquid Level 1 Minimum liquid level The liquid level in the wells or tubes must fully submerge the opening to aspirate using septa piercing tips The distance of the opening is 3 67 mm the minimum liquid level that will be able to be aspirated from the bottom of the well or tube Figure 1 4 Fixed Tip Height and Width Restrictions 1 Height 2 Maximum diameter 1 10 987887AE Span 8 Pod 1 Interchangeable Tips Figure 1 5 Disposable Tip Height and Width Restrictions yu a Se u 1 Height 2 Maximum diameter 987887AE 1 11 Span 8 Pod Interchangeable Tips Figure 1 6 Disposable Tip and Tip Shuck Tube Width Restrictions With Test Tubes F SSS L N H ASA J Nr 1 Tip shuck tube Only the disposable tip portion should descend into test tubes tip shuck tubes should not descend into test tubes or the outside of the tip shuck tube may become contaminated from method solutions Additionally the clearance between the tip shuck tubes and a 10mm test tube is too tight to safely allow the tip shuck tubes to access 10mm test tubes 2 Maximum diameter Accessibility of disposable tips into a test tube is limited to the height of the tip Though it is possible for the tip shuck tube to descend i
61. Figure 2 30 Figure 2 30 Confirmation to Reset Stored Configuration xl 2 Are you certain you want to reset your pod configuration to the values in the stored configuration Settings1 Click Yes to modify the current pod configuration Click No to retain the current pod configuration No 5 Choose Yes The stored configuration is reset N Deleting Pod Settings To delete settings 1 1n Hardware Setup select the Span 8 Pod from the left pane to access the configuration area in the right pane 2 Choose Delete Settings Delete Settings appears Figure 2 31 Figure 2 31 Delete Settings Settings Settings Information Validation Time Not Validated Pump lt None gt Probe Configurations 1 Fixed100 tips 1 mL syringes 2 Fixed100 tips 1 mL syringes 3 Fixed100 tips 1 mL syringes 4 Fixed 100 tips 1 mL syringes 5 Fixed100 tips 1 mL syringes 6 Fixed100 tips 1 mL syringes 7 Fixed100 tips 1 mL syringes 8 Fixed100 tips 1 mL syringes Axis Limit Settings X 211 102 79 Y 5 50 40 00 Span 6 30 14 70 xl 3 Select the desired settings to delete 2 28 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Saving Restoring and Deleting Settings 4 Choose OK The following Confirm appears Figure 2 32 Figure 2 32 Confirmation to Delete Stored Configuration x Are you certain you want to delete the stored configuration Settings1
62. Go is selected the Movement Vector resets to the 0 Auto Clear vector no movement NOTE Auto Clear is on by default Clear Sets movement vector entries to 0 Current Position Current location of the pod and gripper after the pod has been homed Delta Sets the magnitude of change the Vector Builder applies to the Movement Vector for each axis Get Tip Status Indicates which probes have tips Home Z XY Moves Z then X and Y to the home positions LLS Z Move Verifies liquid level sensing by allowing probes to descend and then stop once they hit liquid Move Gripper Z Max Moves gripper to highest configured height Move Z Max Moves pod to highest configured height Movement Vector The amount of movement that occurs when Go is selected Movement vectors are relative to the current position Purge System Removes air from the tubing and purge pump and ensures lines are filled with system fluid Purge Syringes Removes air from the tubing and syringes and ensures lines are filled with system fluid Updates the current position of the pod after it has been physically moved without Retest using Manual Control Set Valve States Sets valves on the pumps to allow system fluid to fill empty or bypass the syringes Speed Sets the speed of the pod with 0 percent meaning use current speed Vector Builder Relative moves are created using the Vector Builder
63. Hardware Manual Biomek NX and Biomek NXP Laboratory Automation Workstation Span 8 Pod 987887AE September 2014 Beckman Coulter Inc 250 S Kraemer Blvd CO aal Brea CA 92821 U S A ULTER Biomek NX and Biomek NX Hardware Manual for the Span 8 Pod Laboratory Automation Workstation PN 987887AE September 2014 2014 Beckman Coulter Inc All rights reserved Trademarks BECKMAN COULTER the stylized logo and Biomek are trademarks of Beckman Coulter Inc and are registered with the USPTO All other trademarks service marks products or services are trademarks or registered trademarks of their respective holders Find us on the World Wide Web at www beckmancoulter com Printed in U S A Revision History This document applies to the latest software listed and higher versions When a subsequent software version changes the information in this document a new issue will be released Initial Issue 03 00 Software version 0 11 Issue B 07 00 Software version 1 0 Issue C 6 03 Changes were made to comply with the EU IVD Directive 98 79 EC change the company name from Coulter Corporation to Beckman Coulter Inc Issue D 3 04 Changes Safety Symbols in Introduction Neutralizing the Waste and Treating for Biohazards in Introduction Heading 4 3 Operational Hazards Specimen Collection and Mixing in chapter 5 Running Cell Controls to Verify Calibration in chapter 5 Running Who
64. Ra podfzo ei2 foss fo Gripper 5 fo p fp ee 1 2 3 4 5 6 7 8 keress a eae i coe zemo 6 061 6 061 6 061 6 061 6 061 6 061 6 061 mae g 5 woe nics ow p p p own Movement Vector Gripper X cm cm Span mm fcm Z cm Spin Grip cm za ee C a a ll aA V3 M4 WS WE M Me rile zoo ff FF F FF z z lt owp of pP p pP p p pP Right OLE X cm cm Span mm Pod p fon fi z k sui Speed 100 I LLS Z Move D pl fi tcm Z2 cm Spin Grip cm Gripper fo 1 f 1 fi fo 1 Close NOTE Do not select Close if Advanced Manual Control closes the framing process must be completed again beginning with step 1 Figure 3 28 Moving Pod Into Gripping Position Frame Gripper x Manually move the pod into gripping position and hit OK Cancel 987887AE 3 31 Framing Instructions Framing the Span 8 Gripper 9 Use Manual Control to move the gripper until it is aligned with the bottom of the AccuFrame Figure 3 29 and squeeze the gripper fingers until they firmly touch the AccuFrame inside the notches at the bottom Figure 3 30 Both front and rear gripper fingers must touch at the same time when squeezing which usually necessitates a move along the Y axis If necessary refer to CHAPTER 4 Manually Controlling the Biomek NX in Biomek Software Figure 3 29 Gripper Framing position on the AccuFrame 987887AE Framing Instruct
65. Size 0 9 cm Additional Roving Height 0 75 cm System Trailing Airgap S uL Post Run Wash Volume 1 mL Always Move to Z max when roving Y Gripper Settings BiomekNX Span 1 Select a Purge Pump for the Span 8 Pod 6 Choose W Accept Hardware Setup closes The Purge Pump is configured for use Correlating the Z Axes Do not use Correlate Z Axes in Hardware Setup without contacting a Beckman Coulter Representative To eliminate mechanical variances the Z axes on the probes must be correlated This procedure is performed during initial setup by a Beckman Coulter Representative in Hardware Setup and should not be repeated Performing Find LLS Sensitivities Performing Find LLS Sensitivities is necessary to test each probe on an individual system to ensure the liquid level sensing capability operates properly This procedure calibrates the system to the individual liquid level sensitivity settings on each probe Although Find Sensitivities is performed during initial setup by a Beckman Coulter Representative the procedure must be repeated when Changing from disposable to fixed tips Changing from fixed to disposable tips 2 10 987887AE Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Difficulties such as false trips or failed senses are experienced using liquid level sensing To perform Find LLS Sensitivities 1 1n Hardware Setup select the Span 8 Pod fr
66. Type FourByThreeHD X cm cm Z cm Precision Podi Coordinates 6 645 13 436 16 4 Not Framed Advanced MC Teach More gt gt Manual Teach Auto Teach In Name verify that the ALP is assigned a unique name Choose Manual Teach Biomek NX Manual Framing Wizard opens with a Warning Figure 3 11 NOTE On the left side of Manual Framing Wizard a list of steps required to complete the teaching process is displayed As the steps of Manual Framing Wizard are accessed the steps are highlighted on the left Figure 3 11 Manual Framing Wizard Warning W Biomek NX Manual Framing Wizard 5 x Warning Warning If you have snapped a continuation manually framing will erase it Press Cancel now to avoid this Clear all other positions of labware and then press Next Technique TeachZ Finish 987887AE Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument 6 Choose Next and Manual Framing Wizard Technique appears Figure 3 12 Figure 3 12 Manual Framing Wizard Technique Selection W Biomek NX Manual Framing Wizard 5 xj Select the technique you would like to use to Frame Technique Frame on deck using Tips C Frame off deck using grippers 7 Select Frame on deck using tips to frame the position by aligning tips to the wells of a microplate OR Select Frame off deck using grippers to frame the position by moving a piece of labware from a prev
67. X in Biomek Software 4 Using Advanced Manual Control with Span 8 Pod and Gripper 6 Enter the desired Y Z Spin and Grip values for the gripper in Movement Vector OR Set the Delta values as desired refer to Setting Delta Values for the Pod and Gripper and click the appropriate buttons on Vector Builder refer to Understanding and Using the Vector Builder until the desired values appear in Movement Vector NOTE Hold the button down to quickly add Delta value to the Movement Vector NOTE Positive values move the pod to the right X and toward the front of the deck Y Positive values also move the probes up Z aspirate D and widen Span the distance between the probes Negative values move the pod to the left X and towards the back of the deck Y Negative values also move the probes down Z dispense D and narrow Span the distance between the probes Minimum and Maximum Y Z Spin and Grip values indicating how far the gripper is able to move are displayed as a tool tip when hovering over the fields in Movement Vector Enter a value in Speed to specify the percent of the pod s maximum speed 8 Choose Go The pod moves from its current position to a new position by the values displayed in the Movement Vector The new positions for the pod and gripper are displayed in Current Position 9 Choose Close to close Advanced Manual Control 10 Choose Exit to close Manual Control Setting Delta Values for the P
68. ability it is recommended that labels be applied to the narrow sides of the microplate This label orientation accommodates scanning of skewed labels and requires less adjustment of the bar code reader position The sections in this appendix include Integrating the Stacker Carousel e Integrating the Bar Code Reader Configuring the Stacker Carousel in Hardware Setup e Framing the Stacker Carousel 987887AE B 1 Using a Stacker Carousel Integrating the Stacker Carousel Integrating the Stacker Carousel NOTE Install only in conjunction with the Biomek NX model incorporating Plexiglas safety shields along the sides of the instrument in lieu of side light curtains Figure B 1 Stacker Carousel Integrating the Stacker Carousel into the system requires e Positioning the Stacker Carousel on the Deck e Mounting the Stacker Carousel e Attaching the Side Shield e Operating the Stacker Carousel B 2 987887AE Using a Stacker Carousel B Integrating the Stacker Carousel Positioning the Stacker Carousel on the Deck The Stacker Carousel shuttle can be positioned on the deck using the deck positions displayed in the Deck Editor by highlighting StackerCarousel Figure B 2 NOTE Refer to the Biomek Software User s Manual PN 987835 Preparing and Managing the Deck for more information on the Deck Editor Figure B 2 Stacker Shuttle Positions Mount Stacker Carousel on Deck Positions that are indicated by dash
69. able tip mandrel Figure 1 19 Figure 1 19 Removing a Tip Shuck Tube From a Disposable Tip Mandrel Probe Tip interface Disposable tip mandrel Tip shuck tube PWN Do not allow the tip mandrel to twist while fastening or removing the collar The collar contains an o ring which turns the tip mandrel if not secured causing the tubing to get twisted or kinked 3 Hold the tip mandrel firmly in place while unscrewing the collar counterclockwise from the bottom of the tip interface 987887AE 1 29 Span 8 Pod Interchangeable Tips 4 pull the collar down and off the disposable tip mandrel Figure 1 20 Figure 1 20 Removing the Collar From a Disposable Tip Mandrel Probe Tip interface Disposable tip mandrel Collar PWN 5 Gently pull the tubing and disposable tip mandrel down from the tip interface until enough tubing is extended to grasp between thumb and forefinger Figure 1 21 1 30 987887AE 987887AE 6 7 IN Span 8 Pod 1 Interchangeable Tips While holding the disposable tip mandrel in one hand gently pull the tubing off the mandrel with the other hand Figure 1 21 NOTE Since the tubing is Teflon it may be difficult to hold Holding the tubing with a Kimwipe may prevent slipping NOTE Carefully twisting the mandrel while pulling on the tubing may ease removal of the mandrel Figure 1 21 Detaching a Disposable Tip Mandrel From the Probe Tubing Probe Tip interfac
70. act a Beckman Coulter Representative Perform the troubleshooting techniques provided in Table 2 when necessary In the case of any other instrument related problems contact a Beckman Coulter Representative NOTE For troubleshooting information regarding the Span 8 Pod refer to CHAPTER 1 Troubleshooting Table 2 Troubleshooting the Biomek NX Instrument If Then All indicator lights are out Check fuses The power is on but the system does not run Check fuses The fuses appear burned or filaments are broken Change fuses refer to Changing Fuses All indicator lights are out the power is on the tact a Beckman Iter Representative fuses are okay and the system does not run Conte che Berkman E Our ene pipes ntalve Power is lost to Span 8 Pod Contact a Beckman Coulter Representative Y axis motion is choppy Contact a Beckman Coulter Representative xxxii 987887AE Introduction Troubleshooting Table 2 Troubleshooting the Biomek NX Instrument If Then A grinding or growling noise is heard Contact a Beckman Coulter Representative Refer to CHAPTER 1 Troubleshooting for more Experiencing problems relating to the Span 8 Pod ihforiation Clean light curtain panels as described in Light Curtain Constant light curtain errors even when no Maintenance violation Contact your Beckman Coulter Representative Changing Fuses Do not remove tower
71. aming ALP and labware positions on the Biomek NX deck Figure 3 1 Framing using the AccuFrame tool requires no human judgment of alignment and is reproducible 987887AE 3 1 Framing Instructions Framing Deck Positions on the Biomek NX Instrument Using AccuFrame 3 2 Figure 3 1 AccuFrame Framing Tool AccuFrame Light Beams Hard Stop Power Light AccuFrame Light Beam Indicators Gripper Cutout Gripper is framed in the cutout at the bottom of both sides of the frame tool 1 2 3 4 5 The AccuFrame fits snugly on an ALP and a teaching process is performed through Biomek Software to obtain the coordinates for each deck position The framing is completed by breaking two light sensors on the AccuFrame at their intersection point The coordinates for each ALP are generated automatically through the software based upon teaching one position however when precision is critical as when using 384 well plates frame each position before using the Biomek NX instrument This ensures that the pod and gripper locate each position reliably There are three indicator lights on the AccuFrame e First light indicates that the AccuFrame is powered on e Middle light indicates the framing status of the Y axis Third light indicates the framing status of the X and Z axes NOTE The AccuFrame is calibrated by a Beckman Coulter Representative The calibration values are stored on the AccuFrame and read as needed by Biomek Software
72. aming Tool 3 2 Warning to Address Before Homing Process Begin 3 4 Framing Deck Positions with a Span Pod 3 5 Attaching a Framing Shaft Details 3 6 Deck Editor 3 8 Position Properties 3 8 Confirm 3 9 Teaching Instructions 3 9 Deck Editor 3 11 Position Properties for a Positive Position ALP 3 12 Manual Framing Wizard Warning 3 12 Manual Framing Wizard Technique Selection 3 13 Manual Framing Wizard if Tips are Not Already Loaded 3 14 Manual Framing Wizard if Tips are Loaded 3 15 Framing to the Corner of Four Wells 3 16 Framing to the Center of a Well 3 17 Manual Teaching the X and Y Axes 3 18 Manual Teaching the X and Y Axes 3 20 Manual Teaching Teach Z 3 22 Manual Framing Wizard Setup for Framing with Gripper 3 24 Manual Framing Wizard Teach X Y with Gripper 3 25 Manual Teaching the X and Y Axes 3 26 Using Delta Values and Directional Buttons to Position Microplate 3 27 Hardware Setup 3 29 Warning to Make Sure Gripper is Ready for Framing 3 30 Pick Previously Framed Deck Position to Frame the Gripper 3 30 Manual Control for Moving Gripper During Framing 3 31 Moving Pod Into Gripping Position 3 31 Gripper Framing position on the AccuFrame 3 32 Correct and Incorrect Gripper Framing Alignment 3 33 3 31 3 32 3 33 3 34 4 1 4 2 4 3 4 4 45 4 6 4 7 4 8 4 9 4 10 4 11 4 12 4 13 B1 Ba B 3 B 4 B 5 B 6 Biz B 8 B 9 B 10 B 11 B 12 B 13 B 14 B 15 B 1
73. ap when accessing tips 1 13 specifications Biomek NX system A 1 Stacker Carousel Biomek NX attaching alignment bracket B 5 attaching side shields B 7 attaching stacker B 7 framing B 29 integrating B 1 mounting B 5 operating B 12 positioning the B 3 supply container defined 1 44 exchanging 1 45 overview 1 41 syringes assembling 1 34 cutoff velocity 1 39 defined 1 33 disassembling 1 37 how size affects pipetting accuracy 1 34 how they affect pipetting accuracy 1 33 installing 1 34 optimizing life expectancy 1 40 optimizing performance 1 40 removing 1 37 sizes supported 1 34 system fluid defined 1 41 degassing 1 51 overview 1 41 priming the system with 1 51 purging the tubing and syringes of air with 1 52 teaching framing instructions 3 1 Stacker Carousel B 29 using Manual Teach 3 11 tower changing fuses in 0 xxxiii definition 0 xxvi troubleshooting 0 xxxii tubing defined 1 43 overview 1 41 W waste container defined 1 48 exchanging 1 49 overview 1 41 wrist ground strap changing tips 1 13 987887AE Beckman Coulter Inc Warranty and Returned Goods Requirements All standard Beckman Coulter Inc policies governing returned goods apply to this product Subject to the exceptions and upon the conditions stated below the Company warrants that the products sold under this sales agreement shall be free from defects in workmanship and
74. ar From a Disposable Tip Mandrel 1 30 1 21 Detaching a Disposable Tip Mandrel From the Probe Tubing 1 31 1 22 Span 8 Pod Pump Assembly Front View 1 32 1 23 Pump and Syringe 1 33 1 24 Installing Syringes Assembling the Syringe 1 35 125 Installing Syringes Push the Syringe Up into the Valve and Screw it in 1 36 1 26 Installing Syringes Push the Thumbscrew Up 1 37 1 27 Removing Syringes Loosen the Thumbscrew and Pull Down 1 38 1 28 Removing Syringes Unscrew and Pull Down on Syringe 1 38 1 29 Removing Syringes Pulling the Syringe Out of the Glass 1 30 131 1 32 133 1 34 1 39 Zal 22 2 3 2 4 2 5 2 6 2 7 2 8 2 9 2 10 2 11 2 12 2 13 2 14 219 216 2 17 2 18 2 19 2 20 221 RAR 2 23 2 24 2 29 2 26 Illustrations Casing 1 39 Supply Container and Tubing Bundle 1 45 Supply Tubing Bundle Upper 1 46 Supply Tubing Bundle Lower 1 47 Waste Container and Tubing 1 49 Waste Tubing Bundle 1 50 Factory Installed Gripper Tool 1 53 Hardware Setup for a Biomek NX Span 8 Instrument 2 2 Hardware Setup Showing the Configuration View for a Span 8 Pod 2 5 Warning on Hardware Setup That Pod Must be Homed and Find Sensitivities Performed When Tip Types are Changed 2 6 Warning That the Pod Must be Homed and Find Sensitivities Performed When Tip Types are Changed 2 6 Hardware Setup 2 7 A Purge Pump Added to the Hardware Setup 2 8 Right Clicking Devices to Add a Pu
75. azardous substances or elements The e stands for electrical electronic and environmental electronic information products This logo indicates that this electronic information product does not contain any toxic or hazardous substances or elements and is green and environmental The outer circle indicates that the product can be recycled The logo also signifies that the product can be recycled after being discarded and should not be casually discarded A43048 AA 987887AE xi Safety Notice RoHS Notice xii 987887AE Contents a Revision History iii Safety Notice v Overview v Alerts for Warning Caution Important and Note v Instrument Safety Precautions vi Electrical Safety vii High Voltage vii Laser Light vii Disposal of Electronic Equipment viii Chemical and Biological Safety viii Moving Parts x Cleaning x Maintenance x RoHS Notice xi China RoHS Caution Label xi China RoHS Environmental Label xi Introduction xxv Overview XXV Configurations for the Biomek NX Span 8 xxv Control Modes xxv System Components xxvi Towers XXvi Indicator Lights xxviii Span 8 Pod xxix Deck xxix ALPs xxix Optional Devices xxix Device Controller xxix Protective Barriers Xxx Light Curtain Protection System xxx Side Safety Shields xxxi xiii Contents xiv Preventive Maintenance xxxi Light Curtain Maintenance xxxii Troubleshooting xxxii Changing Fuses xxxiii Span 8 Pod
76. be saved restored and deleted using Save Settings Restore Settings and Delete Settings refer to Saving Restoring and Deleting Settings Do not change axes limits without contacting a Beckman Coulter Representative 2 23 2 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Enabling Purge Settings The purge settings of a Span 8 Pod are enabled via Re Establish Air Gap and initially configured by a Beckman Coulter Representative in Hardware Setup These settings should not be enabled or modified without specific instructions from a Beckman Coulter Representative When Re establish Air Gap is enabled and configured the behavior of the Span 8 Pod is noticeably different during liquid pipetting Depending on the Cycle Threshold setting the pod moves to the Span 8 Tip Wash ALP during pipetting operations to purge liquid and air to re establish an acceptable air gap If using disposable tips they will be shucked and new tips loaded Do not make any changes to the purge settings without consulting a Beckman Coulter Representative Table 2 3 describes the purge settings listed in Hardware Setup for a Span 8 Pod Table 2 3 Purge Settings Property Description When checked allows the purge settings to be enabled and configured to allow Re establish Air Gap an acceptable air gap to be re established Cycle Threshold Number of aspirate and dispense cycles before the air gap is re established
77. ble 20uL Span_8_20uL No on syringe size Disposable 200uL LLS AP96_200_LLS Yes maximum of 220 uL liquid capacity up to 240 uL air capacity depending Disposable 200uL Span_8_200uL No on syringe size maximum of 20 uL liquid and or air Disposable 20uL barrier Span_8_20uL_ Barrier No i j i capacity depending on syringe size maximum of 125 uL liquid and or air Disposable 200uL barrier Soan_8_200uUL_Barrier No i a capacity depending on syringe size i i f 20 uL liqui i Disposable 20uL LLS Span 8 20uL LLS Barrier y merimum of 20 p iquid anidar air barrier capacity depending on syringe size i i f 125 uL liqui i Disposable 200uL LLS Span 8_200uL LS Barrier ye maximum 0 a iquid angor air barrier capacity depending on syringe size 1 Span 8 Pod Interchangeable Tips Table 1 2 Span 8 Tip Types Tip Name LLS CD EbMyne Software Capable Tip Volume Disposable P1000 uL Span8_P1000_LLS Yes maximum of 1050 uL liquid and or air sterile and non sterile capacity depending on syringe size Disposable P1000 uL Span8_P1000_LLS_Barrier Yes maximum of 1025 uL liquid and or air barrier tips capacity depending on syringe size a Coated refers to tips covered with a Teflon coating The coating assists in preventing cross contamination Solutions are less likely to adhere to the Teflon coating than to uncoated stainless steel fixed tips and are easier to wash off thereby reducing the potential
78. buttons and delta value refer to Using the Delta Value and Directional Buttons d Using the Delta Value and Directional Buttons The microplate can be positioned manually using the delta values and directional buttons to align it with the position being framed Figure 3 23 Pressing one of the directional buttons moves the gripper in that direction by the distance specified in Delta Use the directional buttons and delta values to place the microplate appropriately in the position being framed Figure 3 23 Using Delta Values and Directional Buttons to Position Microplate CH 151 x The labware should be 2 cm above the position Lower the labware into the correct position Press Next gt when pal J the labware is correctly seated in the position fies Delta 0 05 cm O Technique E U ras Back Left 4m gt Bight 0 a Fude Down 5 Total Moved From Start cm We i Finish 1 Delta Value The magnitude of change applied to the tips each time a directional button is selected 2 Directional Buttons The directional buttons move the head assembly by the amount shown in Delta with each press of a button 3 27 Framing Instructions Framing the Span 8 Gripper To use the delta value and directional buttons to position the microplate 1 In Delta select the magnitude of change applied to the gripper each time a directional button is selected Figure 3 18 The defa
79. ch time a Vector Builder button is pressed the Delta value for that axis is added or subtracted from the appropriate Movement Vector for the pod With the point of reference at the front and center of the unit positive and negative values for the pod are as follows Figure 4 8 Vector Builder for the Span 8 Pod Vector Builder Up t Back ij T Aspirate Dispense Left 4m Pam 2 ooo a Widen ew 7 Down Right Positive value X right Left Negative value X left motion motion of the pod of the pod Fwd Positive value Y forward Back Negative value Y back motion of the pod motion of the pod Up Positive value Z up motion of Down Negative value Z down the probes motion of the probes e Aspirate Positive value D Dispense Negative value D aspirating motion of the probes dispensing motion of the probes e Widen Positive Span widening Narrow Negative Span motion between the probes narrowing motion between the probes Use the Gripper buttons Figure 4 9 to add positive or negative values to the Movement Vector for the gripper Each time a Gripper button is pressed the Delta value for that axis is added or 4 12 987887AE Manually Controlling the Biomek NX in Biomek Software 4 Using Advanced Manual Control with Span 8 Pod and Gripper subtracted from the appropriate Movement Vector for the gripper With the point of reference at the front and center of the unit positive
80. cuFrame from the instrument deck 1 Turn off power to the Biomek NX instrument before connecting the AccuFrame 2 Plug AccuFrame into an available CAN port on the Biomek NX tower Make sure the light curtain is not violated by the AccuFrame cable If the light curtain is violated the framing process halts immediately Make sure the AccuFrame cable does not interfere with pod movement Ww Turn on power to the Biomek NX instrument Manually place the AccuFrame onto the ALP position that requires framing by placing the back right corner first and pushing the AccuFrame gently down onto the ALP position NOTE The deck is usually framed from left to right starting at the back left position However ALPs may be framed in any order 5 Make sure that the AccuFrame is fully seated along the posts along the top and right sides of the position NOTE Some ALPs require a framing adaptor to accommodate using AccuFrame to frame the position Make sure the correct adaptor is used for each type of ALP Framing proceeds as normal once the AccuFrame is placed on the appropriate adaptor Framing the Position To frame the Biomek NX deck position NOTE Some ALPs require additional or slightly modified procedures to frame properly Consult the ALPs User s Manual PN 987836 for the specific ALP to frame for any special framing instructions 987887AE 3 7 Framing Instructions Framing Deck Positions on the Biomek NX Instrument Using Acc
81. d 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Stop The pod stops moving N Viewing the Firmware Version Get Version shows the current firmware version for installed devices pods and main firmware To view the firmware version 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Get Version The firmware version displays in an Information dialog similar to Figure 4 6 Figure 4 6 Firmware Version Information Information Ed A Master Controller 2 37 Left arm XY Board 0 7 15 BL 1 5 2 Left arm ZD Board 0 7 15 BL 1 5 2 Right arm XY Board 0 7 15 BL 1 5 2 Right arm ZD Board 0 7 15 BL 1 5 2 3 Toclose Information choose OK Accessing Advanced Manual Control To access Advanced Manual Control 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 987887AE 4 5 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper 2 Choose Advanced Controls 3 select a device from the menu Advanced Manual Control for the selected device appears a Using Advanced Manual Control with Span 8 Pod and Gripper Use Advanced Manual Control Figure 4 7 for the Span 8 Pod and gripper to Viewing the Current Position of the Pod and Gripper Moving the Pod and Gripper to a Safe Roving Height Performing Relative Moves for the Pod and Gr
82. d1 Advanced Manual Control for the pod appears Figure 4 7 The current position is shown in Current Position 4 Choose Close to close Advanced Manual Control Choose Exit to close Manual Control Moving the Pod and Gripper to a Safe Roving Height 987887AE Use Move Z Max and Move Gripper Z Max in Advanced Manual Control to move the pod and or gripper to its highest configured height This helps to avoid but does not completely eliminate the possibility of collisions when moving the pod around the deck manually 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the pod appears Figure 4 7 4 Choose Move Z Max to move the pod to its highest configured height 5 Choose Move Gripper Z Max to move the gripper to its highest configured height 6 Choose Close to close Advanced Manual Control for the selected pod 4 9 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper Choose Exit to close Manual Control N Performing Relative Moves for the Pod and Gripper Relative moves allow the pod to move from its current location to anywhere on the deck Relative moves are created in the Vector Builder using the Delta values or the vector can be manually edited in Movement Vector NOTE Use Auto Clear when the values in Movement Vector must be reset to zero after the mov
83. de a vacuum for pipetting wash tips and perform bulk dispense operations Figure 1 2 Span 8 Pod with Gripper Detailed View 1 Probes 4 Tips are attached to 2 Span 8 Pod the probes at the tip 3 Tip interfaces interface Pod Movements 987887AE The Span 8 Pod performs movements in the Y Z D and S axes see Table 1 1 Table 1 1 Span 8 Pod Axes Movement Movement Y The probes move simultaneously front to back Z the probes move up and down independently D The aspirate dispense action is controlled independently by the pumps The span or distance between the eight probes can expand and collapse NOTE the span between each of the eight is always equal 1 Span 8 Pod Probes Probes Commands entered via Biomek Software on the host computer control the operations of the Multichannel Pod Manual control associated with the operation of the Multichannel Pod is accessed through Biomek Software NOTE Refer to CHAPTER 4 Manually Controlling the Biomek NX in Biomek Software for information on manually controlling the Multichannel Pod The Span 8 Pod uses eight probes Figure 1 2 that can move independently in the Z axis and pipette independently in the D axis with the assistance of the pumps Movement in the S axis provides a span between the probes The pipetting action of the Span 8 Pod is accomplished using either fixed or disposable tips
84. de of the right tower Figure 2 987887AE 987887AE Introduction System Components Communication hookup to host computer via RS232 on the outside of the right tower Main power fuses for the instrument on the outside of the right tower Pneumatically regulated air supply ports for active ALPs outside of the left tower Figure 3 e Electrical plug ins and CAN connections for active ALPs on the outside of both towers and inside of the right tower Figure 4 Figure 2 Right Tower Outside 1 Main power switch 2 Fuses Figure 3 Left Tower Outside C AIR IN AIR OUT AIR OUT N MIN WW 60 psi 414 kPa 40 psif 110 psi MAX 275 kPa 758 kPa 100 psi 690 kPa o CAN PORT CAN PORT xxvii Introduction System Components Figure 4 Right Tower Inside CAN PORT CAN PORT Indicator Lights A status indicator panel with green and amber indicator lights is built into the main cover and indicates the current operational status of the Biomek NX and light curtain refer to Light Curtain Protection System Table 1 defines the indicator light and the operational status each represents Table 1 Indicator Light Key Light Indicator State Operational Status System is functional and in a ready state It is safe to access the instrument Solid Green Idle l fa i and deck without violating the light curtain protective zone Planned Pause This may be written into a method to allow p
85. dware Setup Figure 2 1 is necessary to properly install configure and remove devices Table 2 1 lists and de scribes the toolbar options in Hardware Setup Table 2 1 Hardware Setup Options Options Description Reconnect Allows Hardware Setup to reexamine the devices present Choose this option to determine what devices are present rather than closing and reopening Hardware Setup Home All Axes Provides the Biomek NX Span 8 instrument with a point of reference from which to make subsequent moves Home position is left back NOTE The Span 8 Pod should be homed each time the Biomek NX instrument is powered on Add Device Installs a device 2 2 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Configuring the Biomek NX Span 8 Instrument Table 2 1 Hardware Setup Options Options Description Remove Device Removes a device Saves all changes to the instrument and closes Hardware Setup Choose this option Accept after the device has been installed and configured Configuring the Biomek NX Span 8 Instrument The Biomek NX Span 8 instrument is configured in Hardware Setup to relate information about pod configuration to the software and to specify the communications port to which the instrument is connected To configure the Biomek NX instrument 1 From Hardware Setup select Biomek NX from the left pane The configuration view appears in the r
86. e Tubing Disposable tip mandrel PWN Repeat steps 1 through 6 for any other probes requiring disposable tip mandrels 1 31 Span 8 Pod Pump Assembly Pump Assembly The pump assembly comprises eight pumps and syringes one for each of the probes in the Span 8 Pod Figure 1 22 The pumps and syringes of the pump assembly provide a vacuum which allows the aspirate and dispense actions of the Span 8 Pod Figure 1 22 Span 8 Pod Pump Assembly Front View EN EN 1 Pump Pumps The pumps control the flow of system fluid into the syringes tubes and probes of the Span 8 Pod Figure 1 23 The amount and direction for the flow of system fluid is controlled by setting the state of the valve in the pump The valve can be set to one of three states Input Opens the valve and allows system fluid to flow to and from the syringe with the supply container as the source destination of the fluid Output Opens the valve and allows system fluid to flow to and from the syringe with the tip as the source destination of the fluid Purge Opens the valve and allows the purge fluid to flow through the valve without activating bypassing the syringe NOTE The state of a valve can be changed in Advanced Manual Control if necessary refer to Setting Valve States 1 32 987887AE Span 8 Pod 1 Pump Assembly Figure 1 23 Pump and Syringe 1 Purge Fluid 5 Output
87. e configured to use disposable tips will have to be loaded with a conductive tip Update Settings The currenttip confiquration is Probe 1 Disposable tip No tip configured no tip detected Probe 2 Disposable tip No tip configured no tip detected Probe 3 Disposable tip No tip configured no tip detected Probe 4 Disposable tip No tip configured no tip detected Probe 5 Disposable tip No tip configured no tip detected Probe 6 Disposable tip No tip configured no tip detected Probe 7 Disposable tip No tip configured no tip detected Probe 8 Disposable tip No tip configured no tip detected 3 Choose Next Find Sensitivities Load Tips appears Figure 2 12 NOTE If tips that were not configured in Hardware Setup are detected or if there are probes that must be calibrated Find Sensitivities Tip Configuration appears Figure 2 13 If probes are not calibrated configure the disposable tip in Find Sensitivities Tip Calibration to match the tip that is currently attached to the probe Choose Next to proceed through the wizard 2 12 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Configuring the Span 8 Pod Figure 2 12 Find Sensitivities Load Tips Find Sensitivities Stat Load AP36_200uL_LLS tps orto mance BBE Load Tips al Postion PS and urlosd them z once tesling iz completed Cick on a deck postion to select the lip losd position Update Seti
88. e Preventive Maintenance Troubleshooting 987887AE 1 1 Span 8 Pod Overview Figure 1 1 Span 8 Pod with Factory Installed Gripper Tool on a Biomek NX Instrument 1 Factory Installed Gripper Tool 2 Probes on the Span 8 Pod Main Components of the Span 8 Pod The Span 8 Pod houses the operating mechanisms communications and electrical connections to the base unit and moves the probes in the D Z and Y axes see Table 1 1 for liquid handling functions Figure 1 2 The main components of a Span 8 Pod are Figure 1 2 Probes Hold fixed or disposable tips both conductive capable of Liquid Level Sensing and Clot Detection and non conductive used to perform microplate to microplate test tube to microplate and test tube to test tube liquid transfers refer to Interchangeable Tips probes move in the Y Z S and D axes see Table 1 1 Interchangeable Tips Fixed or disposable tips that are attached to the Span 8 probes used to perform microplate to microplate test tube to microplate and test tube to test tube liquid transfers Pump Assembly Houses the individual pumps and syringes which control the flow of system fluid to and from each of the probes by controlling the D axis see Table 1 1 Figure 1 22 located between the towers at the back of the Biomek NX Span 8 instrument 1 2 987887AE Span 8 Pod Overview Liquid System Stores and transports system fluid used to provi
89. e Z axes in 2 10 defined 2 1 deleting settings 2 28 performing Clot Detection Sensitivities 2 16 performing Find LLS Sensitivities 2 10 restoring settings 2 27 saving setting 2 26 Span 8 Pod properties in 2 22 understanding the options in 2 2 using Accept in 2 3 using Add Device in 2 2 using Home All Axes in 2 2 using Reconnect in 2 2 using Remove Device in 2 3 Home All Axes 2 2 homing all axes of a pod 4 3 pod 3 3 indicator lights defined 0 xxviii instrument graphic of 0 xxvi L light curtain maintenance 0 xxxii status indicator lights 0 xxviii Liquid Level Sensing LLS defined 1 5 disposable tips and 1 22 ensuring accuracy 2 10 fixed tips and 1 14 1 15 how to disable 1 5 1 7 performing Find LLS Sensitivities 2 10 sensitivity settings 1 5 1 6 tips supported 1 5 troubleshooting 1 55 M Manual Control accessing 4 2 defined 4 1 dialog display 4 1 4 2 using 4 1 Manual Teach delta value 3 20 3 27 directional buttons 3 20 3 27 framing Z axis 3 21 using 3 11 using delta values 3 19 3 27 using graphic alignment tool 3 18 3 25 manually framing using the gripper 3 23 Move Z Max using in Advanced Manual Control for a Span 8 Pod 4 9 movement vectors understanding for a Span 8 Pod 4 12 O open architecture instrument design concept 0 xxv optional devices defined B 1 P plate sensor configuring in Hardware Setup 2 25 pod framing teaching Span 8 3
90. e following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure the span gripper is not holding any labware Make sure the span gripper is not near the front back or side of the instrument Home the pod each time the Biomek NX instrument is powered on Homing the pod gives Biomek NX a point of reference from which to make subsequent moves Home position is left back While it is necessary to home the pod after the Biomek NX instrument is powered on it is not necessary to home the pod each time the host computer is turned on or the software is accessed NOTE When attempting to use the pod error messages result until the pod is homed To home the pod Always ensure that the gripper is closed before homing 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Home All Axes The following Warnings and Information appear Figure 4 3 to Figure 4 5 Figure 4 3 Warnings Must Be Addressed Before Homing Process Begins Warning x A CAUTION Before selecting OK to home all axes the following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure the span grip
91. e is performed Turn off Auto Clear to retain the values after the move has occurred Choose Clear to set the values back to zero at any time To move a pod relative to its current position 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the pod appears Figure 4 7 4 Choose Clear to change the Movement Vector values to 0 5 Enter the desired X Y Z D and Span values for the pod in Movement Vector OR Set the Delta values as desired refer to Setting Delta Values for the Pod and Gripper and click the appropriate buttons on Vector Builder refer to Understanding and Using the Vector Builder until the desired values appear in Movement Vector NOTE Hold the button down to quickly add Delta value to the Movement Vector NOTE Positive values move the pod to the right X and toward the front of the deck Y Positive values also move the probes up Z aspirate D and widen Span the distance between the probes Negative values move the pod to the left X and towards the back of the deck Y Negative values also move the probes down Z dispense D and narrow Span the distance between the probes Minimum and Maximum X Y Z D and Span values indicating how far the pod is able to move are displayed as a tool tip when hovering over the fields in Movement Vector 4 10 987887AE 987887AE Manually Controlling the Biomek N
92. e position where tips should be loaded 7 Choose unload them to unload tips when Find Clot Detection Sensitivities is completed OR Choose leave them on to leave tips on when Find Clot Detection Sensitivities is completed 987887AE 2 19 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod 8 Choose Next Tips are loaded Find Clot Detection Sensitivities Choose Position appears Figure 2 22 Figure 2 22 Find Clot Detection Sensitivities Choose Position Select a deck position where the pod can safely determine its Clot Detection Sensitivity This position should not have any labware or other obstructions on it 5 This position should also have a solid top plate Load Tips Choose Position Orbital NOTE If any tips fail to load follow the recovery instructions displayed on Find Sensitivities Load Tips Figure 2 14 9 select a deck position by clicking on the position NOTE The deck position must be an ALP with a solid ground plane for example a 1x3 Passive ALP If using a 1x1 Passive ALP or High Density 4x3 Passive ALP the deck position must have an LLS plate installed 2 20 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Configuring the Span 8 Pod 10 Choose Next Find Clot Detection Sensitivities Test Sensitivities appears Figure 2 23 A few seconds later testing begins a dialog box tracks the test progress Figure 2 24 Figure 2 23 Find Clot De
93. e sure the red laser beam is scanning down across the path of the shuttle If the laser beam is not directed downward toward the shuttle turn off the Stacker Carousel power switch and complete step 2 again If the laser beam is scanning down across the path of the shuttle continue to step 4 4 Make sure the laser scans down through the appropriate tunnel opening For reading labels on the narrow side of the microplate the laser should scan down through one of two slots in the top of the shuttle tunnel For reading labels on the wide side of the microplate the laser should scan down through one of the two slots in the side of the shuttle tunnel 5 Ifthe position of the MS710 needs to be adjusted loosen the screw washer assemblies that attach the bar code reader bracket Figure B 25 to the shuttle tunnel adjust the tilt of the bar code reader until it is aimed properly then tighten the screw washer assemblies 987887AE B 23 Using a Stacker Carousel Integrating the Bar Code Reader 6 To verity correct laser alignment use the pendant to move the shuttle with the labeled microplate through the laser beam The laser beam should pass over all vertical bars on the bar code label simultaneously NOTE Correct and incorrect laser label alignments are shown in Figure B 26 Figure B 26 Bar Code Reader Laser Beam Position Over Bar Code Label G oat 1 Correct Laser Alignment 2 Incorrect Laser Alignment B 24 987887AE U
94. e well When the tip aspirates and rises out of the liquid there is a large change in capacitance detected A clot is detected by determining the change in capacitance as the tip moves up after aspiration Clot Detection Settings Sensitivity settings for clot detection sensing affect the magnitude of change required for conductive tips to detect a clot A smaller sensitivity setting detects smaller changes in the capacitance refer to the Biomek Software User s Manual PN 987835 Setting Technique Values Clot Detecting Tips Use care when handling septa fluted tips they are extremely sharp Use care when handling septa fluted tips they are extremely fragile Biomek NX Span 8 supports the following clot detecting tips Fixed 60 Coated and Uncoated maximum of 5000 uL liquid and or air capacity Fixed 100 Coated and Uncoated maximum of 5000 uL liquid and or air capacity Septa Fluted maximum of 5000 uL liquid and or air capacity NOTE Fixed and septum piercing tips can pull samples into the tubing but the maximum sample volume is limited to syringe capacity refer to Syringes Disposable 20uL LLS up to 100 uL liquid capacity up to 120 uL air capacity Disposable 200uL LLS up to 220 uL liquid capacity up to 240 uL air capacity Disposable P1000 LLS sterile and non sterile up to 1050 uL liquid and air capacity 987887AE Span 8 Pod Interchangeable Tips Disposable P1000 LLS barrier up
95. ed lines in the Deck Editor NOTE While it is possible to pipette liquid to and from a 96 well plate on the Stacker Carousel shuttle it is not possible to pipette liquid to and from a 386 well plate that is sitting on the Stacker Carousel shuttle 987887AE B 3 Using a Stacker Carousel Integrating the Stacker Carousel To position the Stacker Carousel Position the Stacker Carousel outside the Biomek NX deck Figure B 3 B 4 987887AE Using a Stacker Carousel B Integrating the Stacker Carousel Mounting the Stacker Carousel Mounting the Stacker Carousel to the Biomek NX requires attaching an alignment bracket Figure B 4 and a riser plate Figure B 5 Then the Stacker Carousel is positioned and leveled Figure B 4 Attaching Alignment Bracket 1 Use middle front holes for front mount 2 Use middle back holes for back mount Also included with the alignment bracket is the following mounting hardware Two 10 32 x 1 long stainless steel socket head cap screws Two 10 flat washers Alignment bracket Four standoffs Attaching the Alignment Bracket Attach the upper alignment bracket and the riser plate to prepare for positioning and leveling the Stacker Carousel as follows 1 Choose the right or left side of the Biomek NX instrument on which the Stacker Carousel will be located 2 Choose the deck position the shuttle will occupy Figure
96. ent of the bar code reader position B 16 987887AE Using a Stacker Carousel Integrating the Bar Code Reader Position the bracket so the highest edge is parallel with and away from the microplate side to be scanned Figure B 15 through Figure B 25 Figure B 15 Bar Code Reader in Narrow Side Scanning Position om ORO OO OOO E aa OOOO iOO e e a a aa a h DOT OO OOOO a OG CATTOTTOOT OOOO TRESTO ToO 1 Laser Beam Figure B 16 Bar Code Reader in Narrow Side Scanning Position x 0 0 0 00 0090 0000 00000 00000 90 0000 000900 0 K D 6 0 900900 Q OOY O 0 0 Q Q Q Q O fo Q 0 O Q 0 0 xo Q 0 Y o 0 Q 0 0 Q 0 X Q Ko 1 Laser Beam 987887AE B 17 Using a Stacker Carousel Integrating the Bar Code Reader Figure B 17 Bar Code Reader in Wide Right Side Scanning Position 1 Laser Beam Figure B 18 Bar Code Reader in Wide Right Side Scanning Position Bottom View 1 Laser Beam B 18 987887AE Using a Stacker Carousel B Integrating the Bar Code Reader Figure B 19 Bar Code Reader in Narrow Side Scanning Position a p OO CLEE OOOO OOOO a al al a Jel ES OOOO TOTORSD Lote Pan cto el cio te as Yas ip Pl ci Jn lt a COO OOOO
97. eriodic access Blinking Green Pause to the deck When pause is terminated the light curtain is reactivated and the method continues Solid Amber Active A method is running A violation of the light curtain will halt operation Blinking Amber Error Caused by light curtain violation or system error Halts pod and head operations immediately Software communicates the cause xxviii 987887AE Introduction System Components Span 8 Pod Deck 987887AE The Span 8 Pod is a self contained component that holds a series of eight probes that perform liquid handling operations independent of each other Refer to CHAPTER 1 Span 8 Pod for more information on the Span 8 Pod The operation of the pod and probes are controlled by Biomek Software from the host computer The deck Figure 1 is the worksurface of the Biomek NX instrument The stainless steel deck is mounted on a welded tubular frame and is laser etched with standard deck positions The deck also contains predrilled locating holes used to precisely position ALPs refer to ALPs Optional devices refer to Optional Devices can be integrated and positioned within the Biomek NX perimeter making them accessible by the pod NOTE Some ALPs and devices require a Device Controller refer to Device Controller to provide power and communications between the device and Biomek NX instrument ALPs Automated Labware Positioners ALPs are removable and interchangeable platform structur
98. ers Bracket Fastener Collars Bar Code Reader Laser Beam Bar Code Reader E le en Applying Power to the Bar Code Reader To avoid serious damage to the instrument make sure that the laboratory site voltage frequency matches the voltage frequency that was ordered for the instrument Do not attempt to remove or replace covers while the unit is powered on Disconnect power before removing or replacing a cover Apply power to the bar code reader as follows 1 secure the MS710 power cable to the rear panel of the Stacker Carousel NOTE Make sure the cable is connected to the port marked BCR and the cable routing does not interfere with the operation of the Stacker Carousel B 22 987887AE Using a Stacker Carousel B Integrating the Bar Code Reader 2 Turnon the Stacker Carousel power switch N Aligning the Laser Beam Avoid direct exposure to the laser beam Never look directly into the laser beam and never leave the laser on open or unattended Always have the laser module access cover located on the bar code reader in place when operating or troubleshooting the laser module To align the laser beam correctly 1 Verify that the red laser beam is scanning down across the path of the shuttle If the laser beam is not directed downward toward the shuttle turn off the Stacker Carousel power switch N Review Positioning Bar Code Reader before correcting the orientation of the MS710 3 Visually check to mak
99. es that are installed on the deck of the Biomek NX instrument to allow automated assays to be performed ALPs are either Passive ALPs some hold labware in place on the deck while others act as a receptacles for by products from methods such as system fluid and disposed tips tip boxes and labware OR Active ALPs contain mechanisms that may hook to power and or air sources for mechanical operation such as tip washing mixing stirring shaking and precisely positioning labware NOTE Refer to the ALPs User s Manual PN 987836 for more information on ALPs Optional Devices Optional devices such as a Stacker Carousel can be added to the Biomek NX Span 8 instrument to accommodate specific operations Refer to APPENDIX B Using a Stacker Carousel Device Controller A Device Controller is a peripheral box attached to the Biomek NX that provides a means to control a number of high voltage 110VAC 220VAC devices low voltage 24VDC devices and low voltage digital inputs High voltage devices receive power through an AC Power Input while low voltage devices receive power through one of the two Controller Area Network CAN interfaces located on the back of the Device Controller NOTE Refer to the ALPs User s Manual PN 987836 Device Controller for more information on the Device Controller Xxix Introduction System Components Protective Barriers XXX The Biomek NX Span 8 instrument has protective safety measu
100. for cross contamination NOTE Biomek Software does not distinguish between Coated Uncoated fixed tips b To aspirate the maximum volume allowed with fixed tips a 5mL syringe is required The Biomek NX currently supports 100 uL 250 uL 500 uL 1 mL 2 5 mL and 5 mL syringes c Barrier tips are recommended when using disposable tips with liquids that are susceptible to contamination such as blood Labware and Tip Compatibility with the Span 8 Pod The Span 8 Pod is capable of accessing all types of labware supported by the Biomek NX Span 8 instrument however specific types of tips are recommended to access specific types of labware Table 1 3 shows the tips supported by the Span 8 Pod and their accessibility to the different types of labware supported by the Biomek NX instrument NOTE This table is provided as a general reference and does not cover all commercially available microplate types It is the user s responsibility to verify that labware functions properly with the selected tip type For example a flat 384 square well microplate has a larger top cross sectional area than a flat 384 round well microplate therefore Fixed 100 tips can be used to access the flat 384 square well microplate but Fixed 60 tips would be more reliable when accessing the smaller area flat 384 round well microplate Consult your Beckman Coulter Representative for information on labware and tip compatibility not covered in Table 1 2 Use labware and tip c
101. g AccuFrame 4 Choose Auto Teach A Confirm similar to Figure 3 7 appears NOTE This moves the pod to the position being taught The framing probe should be above the AccuFrame in that position Figure 3 7 Confirm 2 The pod is about to go down 5 144 cm and teach position P9 Press OK to continue or Cancel to z abort Cancel 5 Visually verify that the framing probeshaft is positioned to avoid hitting the wall of the AccuFrame and that it is positioned to lower within the AccuFrame tool 6 Choose OK NOTE The pod lowers and moves around inside the AccuFrame automatically until it breaks both light beams simultaneously Figure 3 1 The pod stops after teaching framing is completed and the two light beam indicators are illuminated NOTE An error message appears if both light beams are not broken when the pod is lowered into the AccuFrame If this occurs using Advanced Manual Control refer to CHAPTER 4 Manually Controlling the Biomek NX in Biomek Software move the pod until the probeshaft breaks both light beams and all the indicator lights are on Choose Teach and the pod continues the teaching process The pod may also be moved by hand until the probeshaft breaks both light beams and all the indicator lights are on 7 Wait until the pod stops moving and Teaching Instructions appears Figure 3 8 Figure 3 8 Teaching Instructions Teaching Instructions The location is 58 597 cm 18 751 cm 16 750 cm The c
102. g Disposable Tip Mandrels for more information Liquid level sensing is not working Make sure LLS tips are being used refer to Interchangeable Tips Make sure LLS tips are correctly mounted to the probes refer toFixed Tips and Disposable Tips Mandrels Make sure LLS is enabled in the Technique governing the method Refer to the Biomek Software User s Manual PN 987835 Understanding and Creating Techniques Run Find LLS Sensitivities refer to CHAPTER 2 Performing Find LLS Sensitivities Contact a Beckman Coulter Representative Clot detection is not working Make sure conductive tips called LLS in the Tip Type Editor are being used refer to Interchangeable Tips Make sure conductive tips are correctly mounted to the probes refer to Fixed Tips and Disposable Tips Mandrels Make sure clot detection is enabled in the Technique governing the method Refer to the Biomek Software User s Manual PN 987835 Understanding and Creating Techniques Run Clot Detection Sensitivities refer to CHAPTER 2 Performing Clot Detection Sensitivities Contact a Beckman Coulter Representative Aspirate and dispense actions are not being completed Ensure there is system fluid in the supply container refer to Supply Container 1 Span 8 Pod Troubleshooting 1 56 Table 1 7 Span 8 Pod Troubleshooting If Then Aspirate and dispense actions are inaccurate Ensure tubing connect
103. g a Stacker Carousel Framing the Stacker Carousel To prevent damage to the shuttle first place the AccuFrame into the Framing Tool Adaptor Then place the AccuFrame with the attached adaptor on the shuttle 2 Manually place the AccuFrame into the Framing Tool Adaptor by placing the front right corner first and pushing the AccuFrame gently down into the adaptor Figure B 32 3 Place the AccuFrame with the attached adaptor on the shuttle of the Stacker Carousel so that the locating pins on the bottom of the Framing Tool Adaptor align with the locating holes on the shuttle Figure B 32 Figure B 32 Adding the AccuFrame and Framing Tool Adaptor to the Stacker Carousel Shuttle 1 First gently push the AccuFrame down into the adaptor 2 Then place the AccuFrame with the attached adaptor on the shuttle so the locating pins on the adaptor align with the locating holes on the shuttle B 30 987887AE Using a Stacker Carousel B Framing the Stacker Carousel Turn off power to the Biomek NX instrument before attaching or removing AccuFrame from the instrument deck 4 Turn off power to main unit before connecting the AccuFrame 5 plug the AccuFrame into any available CAN port on the Biomek NX tower Make sure the light curtain is not violated by the AccuFrame cable If the light curtain is violated the framing process halts immediately Make sure the AccuFrame cable does not interfere with pod movement 6 Turnon powe
104. gnized CSA certified Europe 250VAC 3 15 amp 5x20 mm SLO BLO CENELEC approved Communications to Host RS 232 port Fuses 987887AE A 1 Specifications Specifications A 2 Table A 1 Biomek NX Span 8 System Specifications Item Description Communications to Active ALPs CAN Biomek controller host PC The following specifications are the minimum requirements needed for the IBM PC CPU Pentium IV 2 GHz RAM 512 MB Hard Drive 13 5 GB CD ROM Drive 24X Monitor 17 in Super VGA 1024 x 768 small fonts w 16 bit color Operating System Windows XP with Service Pack 1a Other Software SQL Server Personal Edition 987887AE APPENDIX B Using a Stacker Carousel _ Overview The Stacker Carousel Figure B 1 is integrated into the system to dispense and load labware onto the Biomek NX Span 8 deck It provides expanded labware capacity and increases walk away automation in genetic analysis and drug discovery applications Each carousel contains locations for four stackers A shuttle is incorporated for transporting labware to and from the carousel NOTE Fora more comprehensive description of the Stacker Carousel its components functionality and operations refer to the Stacker Carousel User s Manual PN 148598 A bar code reader may be mounted to the Stacker Carousel to scan any one of the four sides of a microplate NOTE For maximum scanning reli
105. graphic alignment tool refer to Using the Graphic Alignment Tool Once the microplate is properly seated in the position being framed choose Next to complete the framing procedure Biomek NX Manual Framing Wizard closes Framing the Span 8 Gripper A Beckman Coulter Representative frames the gripper during system installation however it may be necessary to repeat the framing procedure if extraordinary circumstances occur such as accidentally bending a gripper Before framing the gripper make sure instrument is homed refer to Homing All Axes of the Pods 987887AE 987887AE Framing Instructions Framing the Span 8 Gripper deck position is framed prior to framing the gripper to that location refer to Framing Deck Positions on the Biomek NX Instrument Using AccuF rame e AccuFrame is installed on the appropriate position refer to Installing AccuFrame To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with the factory installed gripper To frame the gripper 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software to start Biomek Software 2 From the Instrument menu choose Hardware Setup Hardware Setup appears 3 Choose the pod to display the configuration information for the pod 4 Choose Gripper Settings to expand the gripper settings The configuration to frame the gripper appears on the right Figure 3 24 Figure 3 24 Hardwa
106. hange is 3 937 cm 0 088 cm 1 050 cm What would you like to do g C Shift ALP Shift position Ca 8 Choose from Shift deck Shift ALP or Shift position for appropriate teaching instructions refer to Selecting Appropriate Teaching Instructions 987887AE 3 9 Framing Instructions Framing Deck Positions on the Biomek NX Instrument Using AccuFrame 9 If the coordinates displayed appear reasonable choose OK Position Properties appears again and the position is framed for the selected pod 10 Choose OK to close Position Properties 11 For framing additional positions move the AccuFrame to the next position to frame refer to Installing AccuFrame and repeat steps 3 through 11 NOTE The framing shaft must be moved to probe 7 to frame positions in the front row of the deck refer to Attaching the Framing Fixture to the Pod 12 Choose Save to save framing information for all positions and close the Deck Editor Figure 3 5 NOTE Choosing Cancel loses all changes to the deck including framing information since the Deck Editor was opened 13 Remove the framing shaft from the probe on the Span Pod N Selecting Appropriate Teaching Instructions In Teaching Instructions Figure 3 8 the entire deck a deck position or an ALP can be shifted by the teaching process Determine what should be shifted using the following information Use Shift deck only before other ALPs or positions are taught
107. he numeric keypad The directional buttons displayed in Manual Teach parallel the keys on the numeric keypad More specifically Fwd correlates to the 1 on the numeric keypad while Down is found on the 2 Left is found on the 4 Right on 6 Up on 8 and Back on 9 4 Visually verify the position of the tips on the pod in relation to the physical position of the wells of the microplate on top of the ALP If the tips are still not accurately positioned above the wells of the microplate on top of the ALP repeat steps 1 and 2 until they are accurately positioned above the target position NOTE The tips may also be aligned with the target position using the graphic alignment tool refer to Using the Graphic Alignment Tool 5 Once the tips are aligned with the target position choose Next to continue Biomek NX Manual Framing Wizard displays Teach Z refer to Framing the Z Axis Framing the Z Axis After the X and Y axes are framed the Z axis must be framed to ensure aspirate and dispense operations are performed at the desired height The final screen in Biomek NX Manual Framing Wizard is for framing the Z axis Figure 3 19 987887AE 3 21 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument 3 22 Figure 3 19 Manual Teaching Teach Z lox The tips should be 0 5 centimeters above the well bottom Lower the pod until the tips just touch the bottoms of the wells until
108. he tubing and syringes of air is accomplished through Manual Control refer to CHAPTER 4 Purging Air from the Syringes and Tubing Purge Pump Gripper 1 52 NOTE The Purge Pump system is now available for the Biomek NX Span 8 system as a field service upgradeable kit PN B47402 Consult your Beckman Coulter Representative for additional details The purge pump is located on the inside of the pump bank The purge pump provides a means of forcefully purging bubbles from the tubing lines and forcefully washing fixed tips The supply line for the purge pump is the 3 8 in tubing line that hangs from the back of the pump bank The purge pump supply line is connected to the supply bottle to use the system fluid as the purge fluid The purge pump pushes fluid through a manifold that splits the flow to each of the eight syringe pump valves During a system purge the syringe pump valves need to be in the purge position that connects the top port to the right side port The system should be purged after sitting overnight and before running each method Purging the system is accomplished through Manual Control refer to CHAPTER 4 Purging Air from the Syringes and Tubing The system can be purged by selecting Purge System in Advanced Manual Control for the Span 8 pod The purge pump can also be used during a method in the Wash Tips or Transfer steps To prevent damage to the gripper do not forcefully move it by hand The Biomek NX may be equipped
109. ight pane Figure 2 1 2 Make sure the serial number listed in Hardware Setup corresponds to the serial number on the Biomek NX instrument 3 Choose the appropriate Port 4 Choose Accept Configuration selections are saved and Hardware Setup closes NOTE An asterisk next to the device indicates the device has been modified since the instrument was loaded NOTE An asterisk next to the device indicates the device has been modified since the instrument was loaded Configuring the Span 8 Pod Configuring a Span 8 Pod in Hardware Setup includes Configuring the Probes for Fixed or Disposable Tips Configuring a Purge Pump e Correlating the Z Axes NOTE Correlating the Z axes should be performed only by a Beckman Coulter Representative at initial setup 987887AE 2 3 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod e Performing Find LLS Sensitivities e Performing Clot Detection Sensitivities Setting Span 8 Pod Properties as instructed by a Beckman Coulter Representative NOTE The settings for a Span 8 Pod can be saved restored and deleted refer to Saving Restoring and Deleting Settings Configuring the Probes for Fixed or Disposable Tips The probes on the Span 8 Pod may be configured for fixed or disposable tips This configuration must be completed in Hardware Setup The probes on the Span 8 Pod must be configured when Changing from disposable tips to fixed tips
110. ings Figure 2 13 Find Sensitivities Tip Configuration Find Sensitivities Start Configure the disposable tips to match the tips thet are currently attached to the probes Itis important that the described configuration matches the physical Tip Configuration configuration otherwise collisions may cccur damaging the device Load Tps Please note thet if you need to switch from a disposable tip to a fixed tip you must exit this wizard and perform that modification in Hardware Setup Choose Postion Probe 1 Tes itivities Probe 2 knne x Update Settings Probe 3 lt None gt hs Probe 4 knon x Pubes Pos a Prbe6 P2001 zl Probe 7 P200_LLS v ProbeS P0 us z NOTE Ifall probes are configured with fixed tips Find Sensitivities Choose Position appears rather than Find Sensitivities Load Tips Go immediately to step 9 4 1n Load choose the appropriate tip type NOTE Any probe configured to use disposable tips must be loaded with a conductive tip 5 in mandrels choose the desired probes 6 click on the position where tips should be loaded 987887AE 2 13 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod 7 Choose unload them to unload tips when Find Sensitivities is completed OR leave them on to leave tips on when Find Sensitivities is completed Choose Next Tips are loaded Find Sensitivities Choose Position appears Figure 2 15 NOTE If any tips fail to load
111. instrumentation If the equipment is used in a manner not specified by Beckman Coulter Inc the protection provided by the equipment may be impaired Alerts for Warning Caution Important and Note 987887AE All Warnings and Cautions in this document include an exclamation point framed within a triangle The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation use maintenance and servicing are attempted WARNING indicates a potentially hazardous situation which if not avoided could result in death or serious injury CAUTION indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment Safety Notice Instrument Safety Precautions Instrument Safety Precautions Risk of operator injury if vi All doors covers and panels are not closed and secured in place prior to and during instrument operation The integrity of safety interlocks and sensors is compromised You con
112. ions 3 Framing the Span 8 Gripper Figure 3 30 Correct and Incorrect Gripper Framing Alignment Correct Gripper Alignment Incorrect Gripper Alignment Bottom of gripper finger Top of gripper finger aligned to aligned to bottom of top of cut out of AccuFrame AccuFrame and aligned to without aligning to inside of the inside of cut out cut out NOTE Assessing the position of the gripper in step 9 is a visual task Figure 3 30 10 once the gripper fingers are properly aligned with the AccuFrame in Frame gripper choose OK Figure 3 28 Frame gripper and Manual Control close The gripper is now framed 11 From Hardware Setup choose Accept Hardware Setup closes 12 From the Instrument menu choose Manual Control Manual Control appears 13 Choose Advanced Controls and select Pod1 Advanced Manual Control appears Figure 3 27 14 Choose Move Gripper Z Max NOTE Choosing Move Gripper Z Max raises the gripper to make the AccuFrame accessible 15 Choose Close to close Advanced Manual Control 16 Choose Exit to close Manual Control 17 Remove the AccuFrame from the ALP position a 987887AE 3 33 Framing Instructions Calibrating Gripper Rotation Testing Gripper Framing Accuracy To make sure the gripper works properly create and run a method at reduced speed around 10 to move a piece of labware from one framed deck position to another If problems occur repeat the gripper framing process until the gripper is working correctly
113. ions are secure refer to Fixed Tips and Disposable Tips Mandrels Ensure fixed tip seating is secure refer to Fixed Tips Ensure disposable tip mandrels are correctly mounted refer to Disposable Tips Mandrels Ensure system fluid and tubing have been purged of air refer to System Fluid Check that cutoff velocities in the technique or liquid type are suitable for the installed syringes refer to Syringe Cutoff Velocities Tips are clipping edges of labware or not able to access labware Reframe the position Check to see if tip or mandrel is obviously bent Contact a Beckman Coulter Representative 987887AE CHAPTER 2 Configuring the Biomek NX Span 8 in Biomek Software Overview The Biomek NX Span 8 must be configured in Biomek Software to ensure proper operation Hardware Setup tells Biomek Software what probes tips and devices to expect on the instrument by providing a connection between the instrument and the software This connection is established by installing configuring and removing devices in Hardware Setup Any active ALPs that require a CAN communication or other devices integrated on the deck of the Biomek NX Span 8 must also be configured in Hardware Setup Refer to the ALPs User s Manual or the specific device integration manual for instructions on configuring the ALP or device in Hardware Setup Do not make any changes to the pod axes limits in Hardware Setup without contacting
114. iously framed position to this position 8 Choose Next to continue to the next screen Biomek NX Manual Framing Wizard displays Setup e refer to Manually Framing Using Tips if framing using tips e refer to Manually Framing Using the Gripper if framing using gripper Manually Framing Using Tips When using high density labware or greater precision is required than the AccuFrame is providing manually frame the position using tips Some positions such as the Span 8 Tip Wash ALP or 8 Channel Active Wash ALP cannot be framed using AccuFrame and must be taught manually Manual framing deck positions using tips involves loading disposable tips to the probes if necessary aligning disposable or fixed tips with the wells of a microplate 987887AE 3 13 3 3 14 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument When Biomek NX Manual Framing Wizard Setup is displayed either Figure 3 13 or Figure 3 14 appears depending on whether or not tips are already loaded onto the pod Figure 3 13 Manual Framing Wizard if Tips are Not Already Loaded E Biomek NX Manual Framing Wizard 4 Load tips from the ZAE i ata iS v tipbox on position Pg v Line tips up against aB 384wellReactionPlate z on position P4 Technique TeachZ Finish 987887AE 987887AE Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument Figure
115. ip cm etect Side Collisions offset 7 783 fo fF f Rotation Calbration M Front i 29 81 JV Back AEE Frame Gripper fe 3 IV Right Set Y Set Grip JIV Enable Plate Sensor Iv Left Maximum BiomekNX Span Figure 2 4 Warning That the Pod Must be Homed and Find Sensitivities Performed When Tip Types are Changed xl A The pod must be homed after changing tip types Failing to home the pod will result in improper framing and inaccurate pipetting 4 To choose the syringe type click on the desired probes to configure from the graphical representation of the probes 5 From the drop down menu choose the syringes in Probes 1 4 use syringes NOTE To choose probes 1 through 8 at one time hold down Ctrl with probes 1 through 4 highlighted and then click on probes 5 though 8 NOTE Probes 1 through 4 may be configured with one size of syringe and probes 5 through 8 may be configured with another size of syringe To mix the size of syringes select probes 1 through 4 and choose the syringe size Then select probes 5 through 8 and choose the syringe size 6 In Disabled Probes choose any probes that should not be used 2 6 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 Configuring the Span 8 Pod 7 Choose Accept Hardware Setup closes b Configuring a Purge Pump The purge pump must be added to the system configuration through Hardware Setup To add a purge pump to the
116. ipper e Performing Absolute Moves for the Pod and Gripper Setting Valve States e Verifying Liquid Level Sensing e Purging Air from the Syringes and Tubing 4 6 987887AE Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper Figure 4 7 Overview Advanced Manual Control for a Span 8 a ft Home Z XY Move Z Max Move Gripper Z Max l Purge Syringes Set Valve States Get Tip Status Purge System Absolute Move 4 G wto Clear G Clear E Refresh Vector g 3 al 2 Left 4 gt Right mee End ame Wier reas Current Position X cm Y cm Span mm owp Pk FFP PP Y cm Z cm Spine Grip cm Movement Vector X cm cn Span mm ae Y cm Z cm Spin Grip cm Mia Vi2 V3 Va Vs Me V7 V8 Z cm o i p p p p p p p D u fo a Down z T End Rotate Left Rotate Right X cm Y cm Span mm eaf pi po z cm 0 1 oo D ut 1 cm Zcm Spin Grip cm 0 1 T LLS Z Move aje p op pF p p p P 1 Home Z XY Moves Z and then X and Y axes to the home positions Move Z Max Moves pod to highest configured height Move Gripper Z Max Moves pod to highest configured height Purge Syringes Removes air from the tubing and syringes Set Valve States Sets valves on the pump to allow system fluid to input output or bypass the sy
117. ips Option To remove the disposable tips option the disposable tip mandrels must be removed from the Span ge Ps Q o D A SPILL HAZARD Use an appropriately contained environment when using hazardous materials Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer Observe cautionary procedures as defined by your safety officer when using toxic pathologic or radioactive materials Always wear protective gloves when removing the tips To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod NOTE Disposable tips should be disposed of into a Span 8 Disposal ALP prior to removal of the disposal tips option NOTE A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tip mandrels on the Span 8 Pod refer to Electro Static Discharge Protection when Handling Tips on the Span 8 Pod 987887AE 1 27 Span 8 Pod Interchangeable Tips To remove the disposable tips option 1 Unscrew the tip shuck tube counterclockwise from the bottom of the tip interface Figure 1 18 Figure 1 18 Fully Installed Disposable Tip Mandrel Probe Tip interface Tip shuck tube Disposable tip mandrel PWN 1 28 987887AE Span 8 Pod 1 Interchangeable Tips 2 Pull the tip shuck tube down and off the dispos
118. isplayed in Information When completed choose OK The air is purged from the tubing and syringes or purge pump 7 Choose Close to close Advanced Manual Control Choose Exit to close Manual Control 4 18 987887AE APPENDIX A Specifications _ Specifications Table A 1 Biomek NX Span 8 System Specifications Item Description Environment Indoor use only Power Requirements 100 240VAC 50 60Hz Air Pressure 60 100 psi of compressed air 414 690 kPa of compressed air i 22 i 0 064 i 414 kP Pneumatic Requirements Air Flow 2 25 cfm 60 psi 0 064 m3 min a NOTE These requirements are necessary only if an ALP used on the instrument requires air the Biomek NX Span 8 workstation does not have pneumatic requirements De ionized or Distilled water System Fluid Requirements Ambient operating temperature is 59 86 F 15 30 C System Fluid should be degassed for 24 hours prior to use 36 in L x 31 in W x 44 in H 91 44 cm L x 78 74 cm W x 111 76 cm H Dimensions of base unit Weight with gripper 190 Ibs 86 2 kg ameno peser ora Humidity Restrictions lt 85 non condensing 86 F 30 C Altitude Restrictions up to 6562 ft 2000m Installation Category Category II Pollution Degree 2 Maximum sound pressure 82 dB Sound Pressure Level Maximum sound pressure at 1 meter away 75 dB US 250VAC 3 15 amp 5x20 mm SLO BLO UL reco
119. k NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper 4 14 4 Choose Absolute Move Absolute Move appears Figure 4 10 Figure 4 10 Absolute Move for Span 8 Pod Absolute Move x Current Position x cm Y cm Span mm fcrm Zecm Spin Grip cm Pod fo o ees gt Gripper E fo fo f wp pp pp Pb bb New Position absolute coordinates Xferm ecm Span mm fcerm Zflcm Spin Grip cm Pod m 9 885 fo Gripper 5 fo fo fo Z cm 2 1 i 1 2 1 2 1 2 1 2 1 2 1 D ul jo fo jo fo fo jo jo fo These values will be converted to the necessary relative move numbers on the Advanced Manual Control Form when you click OK Then press Go to move to these coordinates oe coca 5 InNew Position enter the desired X Y Z D and Span values for the pod NOTE Hover over the fields in New Position to view how far the pod is able to move 6 InNew Position enter the desired Y Z Spin and Grip values for the gripper NOTE Hover over the fields in New Position to view how far the gripper is able to move 7 Choose OK Absolute Move closes and Movement Vector changes to reflect the necessary relative move 8 Enter a value in Speed to specify the percent of the pod s maximum speed to use for the move oose Go The pod moves from its current position to a specified absolute position The new 9 Choose Go The pod from it t position to a specified absolute position Th po
120. le Blood Samples in chapter 5 e Auto Numbering in chapter 5 Manual Sample ID in chapter 5 Scanning the Sample ID with the Barcode Reader in chapter 5 Step 4 in Running Calibration in chapter 7 e Cleaning the Outside of the Instrument and Cleaning the Inside of the Instrument in chapter 8 Steps land 5 in Extended Cleaning Procedure in chapter 8 Step 1 in System Cleaning in chapter 8 Step 1 in Heading 8 4 System Reset Cycle Step 1 in Backflush in chapter 8 Step 1in Bath and Flowcell Rinse in chapter 8 Step 1 in Draining the Baths and or the Diluent Reservoir in chapter 8 Issue AE 09 14 Changes e Added a note to the Purge Pump section Updated the format of this document 987887AE ii Revision History 987887AE Safety Notice Overview Read all product manuals and consult with Beckman Coulter trained personnel before attempting to operate the instrument Do not attempt to perform any procedure before carefully reading all instructions Always follow product labeling and manufacturer s recommendations If in doubt as to how to proceed in any situation contact your Beckman Coulter Representative Beckman Coulter Inc urges its customers and employees to comply with all national health and safety standards such as the use of barrier protection This may include but is not limited to protective eyewear gloves and suitable laboratory attire when operating or maintaining this or any other automated laboratory
121. le finger pad The gripper tool contains two sets of finger pads a double finger pad located to the front of the tool asingle finger pad located to the back of the tool 987887AE 1 53 Span 8 Pod Preventive Maintenance Framing the Gripper A Beckman Coulter Representative frames the gripper during system installation however it may be necessary to repeat the framing procedure if extraordinary circumstances occur such as accidentally bending a gripper To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with the factory installed gripper Should the gripper need to be framed refer to CHAPTER 3 Framing the Span 8 Gripper Preventive Maintenance The Span 8 Pod requires little preventive maintenance however to ensure optimum operation perform the following maintenance procedures as necessary To prevent injury use proper decontamination procedures Make sure all spills are wiped up immediately Return fixed tips mandrels syringes and accessories to their original packaging when they are not in use e Check for leaks at all tubing fittings periodically to make sure that all are tight Check that disposable tip collars are securely tightened to the tip interfaces each week Troubleshooting Perform the troubleshooting techniques presented in Table 1 7 when necessary Do not connect or disconnect any cable while power is applied to the Biomek NX Table 1 7
122. led tubing and container cap are transferred as one unit from the old container to the new container This section also contains complete instructions for bundling the tubing refer to Bundling the Waste Tubing 987887AE 1 49 Span 8 Pod Liquid System Exchanging Waste Containers Without Rebundling the Tubing To exchange waste containers without rebundling the tubing 1 Unscrew the cap from the empty container and remove it and the tubing bundle 2 Insert the tubing bundle into the new container and screw the cap on until hand tight 3 Remove the full waste container and dispose of as directed by the laboratory safety officer NOTE Waste containers can be reused however waste materials contained in the waste container must be disposed of as directed by the laboratory safety officer 4 Position a new waste container in the same location the original waste container occupied NOTE The waste container is positioned at installation to minimize tubing length to keep kinks and bends out of the tubing and to reduce uphill slopes as much as possible Positioning the new waste container in the same location ensures proper alignment of the tubing and maximizes drainage from the Span 8 Tip Wash ALP and drip tray N Bundling the Waste Tubing To bundle the waste tubing 1 Route the tubing from the Span 8 Tip Wash ALP and drip tray through the cap Figure 1 31 Figure 1 34 Waste Tubing Bundle CQ 2 ji 1 Drip Tray Was
123. locities The speed at which fluid is dispensed per second prior to the dispense action abruptly stopping is called the Cutoff Velocity If the Cutoff Velocity in the technique or liquid type governing the pipetting operation is too slow droplets can be left at the end of tips after a dispense pipetting operation Accurately setting the Cutoff Velocity causes the liquid to be ejected at a speed sufficient to prevent droplets from forming on the end of a tip The cutoff velocity should be adjusted in all Span 8 techniques for better pipetting accuracy every time syringes are changed from one type to another When changes to Cutoff Velocity are required they must be made to the technique governing the pipetting operation in the Technique Editor and to the parameters of the fluid used during the pipetting operation in the Liquid Type Editor NOTE Refer to the Biomek Software User s Manual PN 987835 Syringe Cutoff Velocities FX NX S8 only and the Biomek Software User s Manual PN 987835 Changing the Cutoff Velocity for Span 8 Pods in Liquid Type Editor FX NX S8 only 987887AE 1 39 Span 8 Pod Liquid System The minimum and maximum Cutoff Velocity are listed in Table 1 4 The Cutoff Velocities displayed in Table 1 4 are intended as a starting point The values provided should be experimented with to determine the most accurate Cutoff Velocity for a specific liquid handling operation Table 1 4 Span 8 Cutoff Velocities for Syringes
124. lypropylene EPDM and Stainless Steel Quick Disconnect body Polypropylene EPDM and Stainless Steel Tubing Tygon 2075 Cap Polypropylene Carboy Polypropylene NOTE All fitting threads may be wrapped with Teflon tape Tubing Span 8 Pod Liquid System The tubing is a means of transporting system fluid to and from the Span 8 Pod The Span 8 Pod uses 3 8 in tubing from the supply container to the purge pump 1 8 in tubing from the supply container to each syringe pump 1 8 in tubing from the syringe pumps to the probes of the Span 8 Pod and 1 4 in tubing from the pump assemblies overflow to the waste container NOTE The 3 8 in and 1 4 in tubing is Tygon 2075 ultra chemical resistant All 1 8 in tubing is Teflon All diameters refer to outer diameter dimensions 987887AE 1 43 1 Span 8 Pod Liquid System Supply Container 1 44 SPILL HAZARD Do not kink tubing between the supply container the Span 8 pumps and the Purge Pump Do not spill liquids on or around the instrument Wipe up any spills immediately according to the procedures outlined by the laboratory safety officer The supply container is the source of the system fluid used by syringe pumps for the Span 8 Pod and the purge pump Figure 1 30 The supply container attaches to the Biomek NX Span 8 instrument via eight 1 8 in tubing lines from the syringe pumps and a 3 8 in tubing line from the purge pump Inthe sup
125. m The Span 8 Pod uses a liquids system to provide a vacuum for pipetting operations as well as for tip washing and bulk dispense 1 40 987887AE Span 8 Pod 1 Liquid System The liquid system includes System Fluid Acts as a medium for syringes to apply a vacuum used during pipetting operations and is used to wash fixed tips in the Span 8 Pod Tip Wash ALP refer to System Fluid Tubing System fluids are moved through the tubing refer to Tubing Supply Container Source of system fluid for the Span 8 Pod refer to Supply Container e Waste Container Final destination of fluids dispensed to the Span 8 Tip Wash ALP and overflow from the pumps refer to Waste Container System Fluid The system fluid is used to create a vacuum that facilitates the aspirate and dispense actions of the Span 8 Pod System fluid is also used to wash fixed tips positioned in the Span 8 Tip Wash ALP Tap water is not recommended due to high mineral content which could cause blockages in the tubing and increase the possibility of leaks at tubing connections The following liquids are recommended as system fluid for the Span 8 Pod e De ionized water Distilled water NOTE It is possible to use system fluids other than those supported for use with the Span 8 Pod However the corrosive nature of each fluid and its impact on system components along the Span 8 system fluid flow path and in the Span 8 Tip Wash ALP must be considered
126. materials for one year after delivery of the products to the original Purchaser by the Company and if any such product should prove to be defective within such one year period the Company agrees at its option either 1 to correct by repair or at the Company s election by replacement any such defective product provided that investigation and factory inspection discloses that such defect developed under normal and proper use or 2 to refund the purchase price The exceptions and conditions mentioned above are as follows 1 Components or accessories manufactured by the Company which by their nature are not intended to and will not function for one year are warranted only to reasonable service for a reasonable time What constitutes a reasonable time and a reasonable service shall be determined solely by the Company A complete list of such components and accessories is maintained at the factory 2 The Company makes no warranty with respect to components or accessories not manufactured by it In the event of defect in any such component or accessory the Company will give reasonable assistance to Purchaser in obtaining the manufacturer s own warranty 3 Any product claimed to be defective must if required by the Company be returned to the factory properly decontaminated of any chemical biological or radioactive hazardous material transportation charges prepaid and will be returned to the Purchaser with transportation charges collect u
127. n the Biomek NX Span 8 Figure 1 1 The podis a liquid handling tool capable of performing liquid transfers from test tubes and large pieces of labware to smaller pieces of labware or vice versa The Span 8 Pod can also perform liquid level sensing LLS and clot detection CD during liquid transfers when conductive tips are attached to the probes NOTE Conductive disposable tips are identified as LLS in the Tip Type Editor Fixed tips are also conductive The Span 8 Pod performs liquid transfers using a series of eight probes that can move independently in the Z axis pipette independently in the D axis and span from 9mm to 20mm between the probes in the S axis see Table 1 1 The pipetting action of the Span 8 Pod is accomplished using syringe pumps and either fixed or disposable tips with or without liquid level sensing capabilities The Span 8 Pod contains its own electrical cabling and communication with the base unit and it interacts with ALPs located over the entire deck of the Biomek NX The Span 8 Pod may also be equipped with an gripper tool on the left side of the pod that grasps and moves labware from one location on the Biomek NX deck to another The gripper is a factory installed option and may not be installed later The sections in this chapter include Main Components of the Span 8 Pod e Liquid Level Sensing e Clot Detection e Interchangeable Tips Labware and Tip Compatibility with the Span 8 Pod Gripper
128. ning Figure 2 4 appears stating that the pod must be homed and Find Sensitivities performed 2 5 In Hardware Setup select the Span 8 Pod from the left pane to access the configuration area in 2 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Figure 2 3 Warning on Hardware Setup That Pod Must be Homed and Find Sensitivities Performed When Tip Types are Changed Biomek Hardware Setup 2 Reconnect ff Home all Axes b Add Device ms Remove Device Biomek NX SN None types the pod must be homed and Find Sensitivities must be performed rd etd Probe Configuration Click to select which probes to configure amp Devices F DeviceControllerd Last Validation Not Validated Set Validation Time 47 PurgePumpo AA oF ShakerALPO Axis Limit Settings X cm cm Z cm DWL Span cm oF ShakerALP1 oF StirrerALPO Minimum fe fas fis 938 fo jos oF StirrerALP1 x z Ma 102 79 fo 12 1381 fiooo fia 7 7 Tiploadero Probes 1 4 use ea F Tiploader1 A Set Set Y Digital Devices isposable i E Simulator i Gorrelate Pods Correlate Z Ke Find US Sensitivities OD Camera EF Materials Disabled Probes 1 2 3 4 5 6 7 8 Pume Jenone gt a inc Sete Stacker Carousels Save Settings Restore Settings Delete Settings V Additional Pod Settings Gripper Settings IV Gripper Installed X cm cm Z cm Gr
129. nless the product is found to be defective 4 The Company shall be released from all obligations under all warranties either expressed or implied if any product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair by others is made with the written consent of the Company 5 Ifthe product is a reagent or the like it is warranted only to conform to the quantity and content and for the period but not in excess of one year stated on the label at the time of delivery It is expressly agreed that the above warranty shall be in lieu of all warranties of fitness and of the warranty of merchantability and that the company shall have no liability for special or consequential damages of any kind or from any cause whatsoever arising out of the manufacture use sale handling repair maintenance or replacement of any of the products sold under the sales agreement Representatives and warranties made by any person including dealers and representatives of the Company which are consistent or in conflict with the terms of this warranty shall not be binding upon the Company unless reduced in writing and approved by an expressly authorized officer of the Company Parts replaced during the warranty period are warranted to the end of the instrument warranty Warranty 1 Beckman Coulter Inc Warranty and Returned Goods Requirements Warranty 2 NOTE Performance characteristics and s
130. ntive Maintenance 1 54 Troubleshooting 1 54 Configuring the Biomek NX Span 8 in Biomek Software 2 1 Overview 2 1 Accessing Hardware Setup 2 1 Understanding the Options in Hardware Setup 2 2 Configuring the Biomek NX Span 8 Instrument 2 3 Configuring the Span 8 Pod 2 3 Configuring the Probes for Fixed or Disposable Tips 2 4 Configuring a Purge Pump 2 7 Correlating the Z Axes 2 10 Performing Find LLS Sensitivities 2 10 Performing Clot Detection Sensitivities 2 16 Setting Span 8 Pod Properties 2 22 Enabling Purge Settings 2 24 Setting Span 8 Gripper Properties 2 24 Using the Plate Sensor 2 25 Saving Restoring and Deleting Settings 2 26 Saving Pod Settings 2 26 Restoring Pod Settings 2 27 Deleting Pod Settings 2 28 Framing Instructions 3 1 Overview 3 1 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame 3 1 Homing All Axes of the Pods 3 3 Attaching the Framing Fixture to the Pod 3 4 Installing AccuFrame 3 6 Framing the Position 3 7 Selecting Appropriate Teaching Instructions 3 10 Manually Framing Deck Positions on the Biomek NX Instrument 3 11 Manually Framing Using Tips 3 13 Using the Graphic Alignment Tool 3 18 Using the Delta Value and Directional Buttons 3 19 Framing the Z Axis 3 21 Manually Framing Using the Gripper 3 23 XV Contents xvi Using the Graphic Alignment Tool 3 25 Using the Delta Value and Directional Buttons 3 27 Framing the Span 8 Gripper 3
131. nto test tubes larger than 10mm it is not recommended as the tip shuck tube may come into contact with the sides of the test tube and become contaminated 3 10mm test tube 4 Tip height 1 12 987887AE Span 8 Pod 1 Interchangeable Tips Electro Static Discharge Protection when Handling Tips on the Span 8 Pod 987887AE To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tips on the Span 8 Pod or moving the Span 8 Pod or Gripper by hand NOTE Awrist ground strap which must be attached to the instrument deck base plate using the alligator clip is supplied with the Biomek NX Span 8 instrument To attach a wrist ground strap 1 Turn off the power to the Biomek NX Span 8 instrument 2 Position the strap portion of the wrist ground strap snugly around the wrist 3 Clamp the alligator clip of the wrist ground strap to the instrument deck base plate Figure 1 7 1 13 Span 8 Pod Interchangeable Tips 4 Power up the Biomek NX instrument Figure 1 7 Wrist Ground Strap Attached to the Deck zy D ce ao 4 2 P 1 Strap portion of wrist ground strap snugly attached to the wrist 2 Alligator clip clamped to the instrument deck base plate Fixed Tips All fixed tips are capable of LLS
132. od and Gripper A Delta value is the amount of change applied to the Movement Vector when a button in the Vector Builder is pressed For example if the Delta value for X is 3 each time Right is clicked in the Vector Builder 3 cm is added to the X axis of the Movement Vector The move does not occur until Go is selected in Movement Vector of Advanced Manual Control To set Delta values 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the pod appears Figure 4 7 4 11 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper 4 in Pod enter a value for X Y and Span to set the Delta value for each pod axis 5 InZ cm enter a value to set the Delta value for moving probes in the Z axis 6 nD pL enter a value to set the Delta value for making aspirate and dispense movements with the probes In Gripper enter a value in Y Z Spin and Grip to set the Delta value for each gripper axis Understanding and Using the Vector Builder The use of Advanced Manual Control for the Span 8 Pod centers around building and applying movement vectors using the Vector Builder A movement vector indicates the magnitude and direction of motion applied to the pod or probes Use the Vector Builder buttons Figure 4 8 to add positive or negative values to the Movement Vector for the pod Ea
133. om the left pane to access the configuration area in the right pane Figure 2 9 2 Choose Find LLS Sensitivities Find Sensitivities opens with Start Figure 2 10 After a few seconds the tips are detected and Figure 2 11 appears Figure 2 9 Hardware Setup Find LLS Sensitivities Button Reconnect ft Home All Axes Ei Add Device Remove Device J Accept gt lt Cancel B Biomek NX SN Mone j AccuFrame 8 Podi 5 A Devices 7 DeviceControllerO 47 PurgePumpo oF ShakerALPO oF ShakerALP1 oF StirrerALPO oe StirrerALP1 f Digital Devices c e Simulator E Camera OD Materials Stacker Carousels Probe Configuration Click to select which probes to configure Probes 1 4 use Fixed100 x tips 1ml F syringes Disabled Probes 1 2 3 4 5 6 7 8 Additional Pod Settings Y Gripper Settings Save Settings Restore Settings s Delete Settings s Last Validation fot Validated Set Validation Time Axis Limit Settings x cm Y cm Z cm D ul Span cm Minimum 2 11 5 5 fas o38 fo f3 Maximum 102 79 40 fiz iseie fiooo fia Sex sey Gorrelate Pods Correlate 2 Axes Find LLS Sensitivities Pump PurgePumpo x Find cD Sensitivities BiomekNX Span 987887AE 2 11 2 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Figure 2 10 Find Sensitivities Start Find Sensitivities
134. ombinations labeled as Limited with caution Tips could become wedged inside labware picking the labware up with the probe when the probe is raised Table 1 3 Labware and Tip Compatibility Tip Types Labware Disposable Disposable Septa Fixed 100 Fixed60 ap96 200p AP96 20uL Piercing Tips Flat 96 R R R R La R Deep 96 R R R Lb La R Flat 384 LC R Ld R Na N Deep 384 Lc R Ld Lb Na N 1 8 987887AE Table 1 3 Labware and Tip Compatibility Span 8 Pod Interchangeable Tips Tip Types aoe Fixed 100 Fixed 60 Apos a APSE 204 eee ia io se 2mm R Lb R R Ra R een 5_5mm R Lb R R Ra R aa 5_5mm N A NA N A N A Ra N a e N A N A N A N A Re N R Recommended N No Access L Limited Access due to height and or width restrictions See Caution above a Enough liquid must be present in the wells or tubes to fully submerge the side opening to aspirate using the septa piercing tip Figure 1 3 b The tip fixed or disposable is too short to allow the tip to access fluid in the bottom of the labware Figure 1 4 and Figure 1 5 c Fixed 100 tips are designed to fully access test tubes while Fixed 60 tips are designed to increase reliability in accessing high density 384 well microplates d The maximum diameter of the disposable tip is too wide to allow the tip to fully access the labware Figure 1 4 Figure 1 5 and
135. ome all axes the following conditions must be met Make sure there is no liquid present in the tips e Make sure there are no disposable tips loaded e Make sure the Framing Probe is NOT installed on the Multichannel Pod Make sure the grippers on the Multichannel Pod are retracted e Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod e Make sure the span gripper is not holding any labware e Make sure the span gripper is not near the front back or side of the instrument Prior to framing the Biomek NX deck all axes must be homed Homing the pod gives Biomek NX a point of reference from which to make subsequent moves Home position is left and back NOTE Home the pod each time the Biomek NX instrument is powered on When attempting to use the pod error messages result until the pod is homed NOTE While it is necessary to home the pod after the Biomek NX instrument is powered on it is not necessary to home the pod each time the host computer is turned on or the software is accessed To home the pod 1 Choose Start gt Programs gt Beckman Coulter gt Biomek Software to open Biomek Software 987887AE 3 3 Framing Instructions Framing Deck Positions on the Biomek NX Instrument Using AccuFrame 2 Choose Instrument gt Home All Axes Warning appears Figure 3 2 NOTE Additional Warnings and Information may appear depending on the instrument configuration NOTE Choosing Home All Axe
136. on framing the Stacker Carousel Preventive Maintenance and Troubleshooting Refer to the Stacker Carousel User s Manual PN 148598 for information on preventive maintenance and troubleshooting B 12 987887AE Using a Stacker Carousel B Integrating the Bar Code Reader Integrating the Bar Code Reader A bar code reader may be mounted to the Stacker Carousel to scan any one of the four sides of a microplate NOTE For maximum scanning reliability it is recommended that labels be applied to the narrow sides of the microplate This label orientation accommodates scanning of skewed labels and requires less adjustment of the bar code reader position CLASS II LASER PRODUCT THIS PRODUCT CONFORMS TO APPLICABLE REQUIREMENTS OF 21 CFR 1040 AT THE DATE OF MANUFACTURE Integrating the bar code reader requires e Attaching Bar Code Reader Bracket e Attaching Bar Code Reader to Shuttle e Positioning Bar Code Reader Applying Power to the Bar Code Reader e Aligning the Laser Beam Attaching Bar Code Reader Bracket Attach the bar code reader mounting bracket to the bar code reader as follows 1 Turn off the Stacker Carousel power switch 987887AE B 13 Using a Stacker Carousel Integrating the Bar Code Reader 2 Using a Phillips head screwdriver fasten the bar code reader Microscan MS710 to the bracket using two M4 x 6 flat head screws Figure B 11 Figure B 11 Bar Code Reader Shuttle Mount Access Cover Removed
137. once the tip is aligned with the wells of the microplate on top of the ALP choose Next to continue Biomek NX Manual Framing Wizard displays Teach Z refer to Framing the Z Axis Using the Delta Value and Directional Buttons The tip can be positioned manually using the delta values and directional buttons to align it with the physical position of the wells of the microplate on top of the ALP Pressing one of the directional buttons moves the pod in that direction by the distance specified in Delta Position the tips above the wells of the microplate on top of the ALP 987887AE 3 19 3 20 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument Figure 3 18 Manual Teaching the X and Y Axes W Biomek NX Manual Framing Wizard 3 4 loj xj BK waning Delta 005 cm G The tips should be about a half centimeter above the plate Drag the circle to where the tips are in relation to the Well Center and press Go Press Next gt when the tips are directly above their target I Technique P Back Left 4m gt Right A Fude Down B IV Hysteresis compensation Total Moved From Start cm Teach XY 0 0 0 Reset C Well Comer well Center Go TeachZ 1 Delta Value The magnitude of change applied to the tips each time a directional button is selected 2 Directional Buttons The directional buttons move the head assembly by the amount shown in Delta with each pres
138. ons 2 2 Span 8 Pod Properties 2 23 Purge Settings 2 24 Span 8 Gripper Settings 2 25 Troubleshooting Framing 3 37 Advanced Manual Control Selection Areas for the Span 8 Pod Refer to specific subsections for instructional use 4 7 Biomek NX Span 8 System Specifications A 1 xxiii Tables xxiv Introduction a Overview NOTE Unless otherwise noted all information in this hardware manual refers to both the Biomek NX and Biomek NX instruments The Biomek NX Span 8 Laboratory Automation Workstation is a multi axis liquid handling instrument used in the life sciences and bioresearch laboratory The open architecture design along with the extensible operating software provides a foundation for integrating current and future specific use components The design of the Biomek NX Span 8 provides the foundation for continual evolution and expansion of liquid handling systems The Biomek NX Span 8 Pod is available with a gripper tool for moving labware Figure 1 A variety of labware and hardware adapt the deck of the Biomek NX to accomplish multiple tasks ranging from performing simple labware positioning and liquid transfers to completing complex activities that typically require additional devices in the laboratory The capabilities of the Biomek NX depend on the components installed including the pod configuration Automated Labware Positioners ALPs and third party devices Configurations for the Biomek NX Span 8
139. or clot detection when LLS or clot detection is enabled in the technique governing the liquid handling procedure However if LLS or clot detection is disabled in the technique LLS or clot detection is unavailable regardless of the type of tips attached to the probes To use fixed tips they must be physically installed on the probes and configured for use in the Hardware Setup Septa fluted tips capable of piercing septum are also fixed and capable of LLS or clot detection however they are extremely fragile and must be installed carefully 1 14 987887AE Span 8 Pod 1 Interchangeable Tips Installing Fixed tips Use care when handling septa fluted tips they are extremely sharp Use care when handling septa fluted tips they are extremely fragile Do not perform aspirate or dispense operations without mandrels installed and tubing attached to fixed tips To operate the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod NOTE A wrist ground strap must be attached to the instrument deck base plate prior to installing or removing tips on the Span 8 Pod refer to Electro Static Discharge Protection when Handling Tips on the Span 8 Pod To install fixed tips 1 Place the end of a fixed tip into the tubing that extends from the bottom of the Span
140. out actual hardware problems Always Move to Z max when roving Check this field to move the pod to its maximum height during any move across the deck Last Validation Set by a Beckman Coulter Representative using Set Validation Maximum X Y Z D and Span The maximum position the pod may go to along the X Y Z D and Span axes relative to the Home position Set by using the appropriate buttons under the X cm and Y cm columns Do not change the axes limits without contacting a Beckman Coulter Representative Minimum X Y Z D and Span The minimum position the pod may go to along the X Y and Z axes relative to the Home position Set by using the appropriate buttons under the X cm and Y cm columns Do not change the axes limits without contacting a Beckman Coulter Representative Post Run Wash Volume Amount of liquid in mL run through the probes after a method is run Probe Size Thickness of the probe along the Y axis Speed Limit Controls the speed for pod movement based on a percentage of its maximum speed System Trailing Airgap Volume of air between the system liquid and the additional air gaps that are drawn into the tip Unload Speed Controls the pod speed when unloading tips based on a percentage of its maximum pod speed Recommended that this be modified only by a Beckman Coulter Representative NOTE Pod properties for a Span Pod may
141. p to four stackers may be attached to the Stacker Carousel Stackers are identified on the Stacker Carousel as A B C or D Make sure the correct stacker type is selected for each stacker on the carousel Stacker types may be Stacker 10 Stacker 20 or Stacker HD B 28 987887AE Using a Stacker Carousel B Framing the Stacker Carousel 6 Choose Accept Hardware Setup closes NOTE Accept must be chosen after the Stacker Carousel has been configured to allow Hardware Setup to accept the configuration Removing Stacker Carousels To remove Stacker Carousels 1 In Hardware Setup right click the desired device under Stacker Carousels 2 Choose Remove Device The device is removed from the installed stackers under Stacker Carousels in the left pane OR 1 Select the desired stacker under Stacker Carousel 2 Choose Remove Device from the top of Hardware Setup The device is removed the installed devices under Stacker Carousels in the left pane a Framing the Stacker Carousel The Stacker Carousel is framed using the AccuFrame and the Framing Tool Adaptor To frame the Stacker Carousel 1 Using the Control Pendant choose SHUTTLE IN and SHUTTLE OUT to ensure the shuttle of the Stacker Carousel is in the correct position for framing The shuttle should be out for framing NOTE Refer to the Stacker Carousel User s Manual PN 148598 for more information on using the Control Pendant 987887AE B 29 Usin
142. pecifications are only warranted when Beckman Coulter replacement parts are used Except as provided in writing signed by an officer to Beckman Coulter Inc this system and any related documentation are provided as is without warranty of any kind expressed or implied including that the system is error free This information is presented in good faith but Beckman Coulter does not warrant guarantee or make any representations regarding the use or the results of the use of this system and related documentation in terms of correctness accuracy reliability currentness omissions or otherwise The entire risk as to the use results and performance of this system and related documentation is assumed by the user 987887AE Beckman Coulter Inc Warranty and Returned Goods Requirements 987887AE Warranty 3 www beckmancoulter com COULTER 2014 Beckman Coulter Inc All Rights Reserved
143. per is not holding any labware Make sure the span gripper is not near the Front back or side of the instrument 3f Cancel 987887AE 4 3 Manually Controlling the Biomek NX in Biomek Software Using Manual Control Figure 4 4 Warning that the Span Probes are About to Go Down to the Washstation Warnina x The probes are about to go down to the washstation Probes 1 4 have no tips Probes 5 8 have no tips Press OK to continue or Cancel to abort I Cancel Figure 4 5 Information for a Span 8 Pod information x i When the intake is clear of bubbles press OK 3 Confirm that each Warning and Information dialog is addressed appropriately and choose OK to continue homing the pod 4 After clearing Figure 4 5 wait for the pod to stop moving The homing process is complete N Moving a Pod to a Specific Deck Position 4 4 Use Manual Control to easily move the pod to a specific deck position Manual Control moves the pod to the top of the Z axis then centers it over the selected position To move a pod to a specific deck position 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Clickon the desired deck position on the Manual Control Deck Display The pod moves above the selected deck position b 987887AE Manually Controlling the Biomek NX in Biomek Software Using Manual Control Stopping a Pod To stop a pod once a movement has starte
144. ply container the nine lines are bundled together using two tubing retainers a gasket and container cap NOTE Supply containers can be reused when they are filled with a system fluid recommended for the Span 8 Pod refer to System Fluid 987887AE 987887AE Span 8 Pod Liquid System Figure 1 30 Supply Container and Tubing Bundle b dod Cap Gasket Upper tubing retainer Lower tubing retainer A WU NS m Exchanging Supply Containers The supply tubing is initially bundled by a Beckman Coulter Representative when the Biomek NX Span 8 instrument is installed When supply containers are exchanged the bundled tubing and container cap are transferred as one unit from the old container to the new container This section also contains complete instructions fro bundling the tubing refer to Bundling the Supply Tubing Exchanging Supply Containers Without Rebundling the Tubing To exchange supply containers without rebundling the tubing 1 Unscrew the cap from the empty container and remove it and the tubing bundle 2 Insert the tubing bundle into the new container and screw the cap on until hand tight 1 45 1 Span 8 Pod Liquid System 3 Remove the empty container NOTE Supply containers can be refilled with system fluid degassed and then reattached to the Biomek NX Span 8 instrument 4 Position a new supply container in the same location the original supply container occupied NOTE The supply containe
145. position of the wells of the microplate on top of the ALP NOTE The small circle represents the tip on the pod The objective is to provide the software with a representation of the tip s position in relation to the physical position of the wells of the microplate on top of the ALP The software uses this graphical representation to know approximately how far in any direction the pod must move to align the tips with the wells of the microplate on top of the ALP 3 18 987887AE Framing Instructions 3 Manually Framing Deck Positions on the Biomek NX Instrument 2 Select Go The pod moves in accordance with the position of the small circle in relation to the large circle NOTE When the move is completed the small circle resets itself to the center of the large circle The values displayed in Total Moved from Start cm changes each time steps 1 and 2 are completed If desired the values in Total Moved from Start cm can be reset to zero by selecting Reset 3 Visually verify the position of the tips on the pod in relation to the physical position of the wells of the microplate on top of the ALP If the tips are still not accurately positioned above the target position on the microplate repeat steps 1 and 2 until they are accurately positioned above the target position the microplate NOTE The tips may also be aligned with the microplate using the delta value and directional buttons refer to Using the Delta Value and Directional Buttons 4
146. r Representative the procedure must be repeated when e Changing from disposable to fixed tips e Changing from fixed to disposable tips Difficulties such as false trips or failed senses are experienced using liquid level sensing 2 16 987887AE Configuring the Biomek NX Span 8 in Biomek Software 2 To perform Find CD Sensitivities Configuring the Span 8 Pod 1 1n Hardware Setup select the Span 8 Pod from the left pane to access the configuration area in the right pane 2 Choose Find CD Sensitivities Find Clot Detection Sensitivities opens with Start Figure 2 19 After a few seconds the tips are detected and Figure 2 20 appears Figure 2 19 Hardware Setup Find CD Sensitivities Button l Reconnect A Home All Axes Jp Add Device e Remove Device J Accept gt lt Cancel Biomek NX SN None f AccuFrame 8 Podi Ej amp Devices 7 DeviceControllerO 47 PurgePumpo 7 ShakerALPO oF ShakerALP1 oF StirrerALPO oF StirrerALP1 f Digital Devices BP Simulator E Camera OP Materials Stacker Carousels Probe Configuration Click to select which probes to configure Probes 1 4 use Fixed100 x tips 1ml bd syringes Disabled Probes 1 2 3 4 5 6 7 e Save Settings Restore Settings Delete Settings Last Validation fot Validated Set Validation Time Axis Limit Settings x cm cm Z cm Diyl Span cm Minimum fea fs fis 938
147. r Stacker Carousels in the left pane Figure B 30 Installing a Stacker Carousel by Right Clicking Biomek Hardware Setup 5 2 Reconnect ff Home All Axes di Add Device ss fer gt Z Accept X lt Cancel E Biomek NX SN None fj AccuFrame 8 Podi H Devices amp Digital Devices H E Simulator Stacker Carousel BiomekNX Span 987887AE B 27 Using a Stacker Carousel Configuring the Stacker Carousel in Hardware Setup Configuring Stacker Carousels To configure Stacker Carousels 1 In Hardware Setup select the desired stacker under Stacker Carousels in the left pane of Hardware Setup and the configuration view appears in the right pane Figure B 31 Figure B 31 Hardware Setup Showing the Configuration View for a Stacker Carousel Biomek Hardware Setup Reconnect a Home All Axes Add Device Remove Device J Accept gt lt Cancel S See iter Serial Number pooo a AccuFrame 8 Podi Port fa Devices Simulate bt Piva J This device has a Bar Code Reader Simulator Stacker Carousels a Ore q Ore a Gime Ore a BiomekNX Span Enter the correct Serial Number to correspond to the serial number on the Stacker Carousel 3 Choose the appropriate Port Check This device has a Bar Code Reader when a bar code reader has been attached to the Stacker Carousel Choose the appropriate stacker type for each stacker on the carousel NOTE U
148. r is positioned at installation to minimize tubing length and to keep kinks and bends out of the tubing and to reduce uphill slopes as much as possible Positioning the new supply container in the same location ensures proper alignment of the tubing and reduces pipetting errors due to air in the tubing N Bundling the Supply Tubing To bundle the supply tubing 1 Route the tubing from the syringe pumps and purge pump through the cap Figure 1 31 Figure 1 31 Supply Tubing Bundle Upper 1 Syringe Pump 4 Purge Pump Supply Tubing Supply Tubing Optional feature available on the 2 Gasket Biomek NX Purge Pump System only 3 Cap 5 Upper Tubing Retainer 1 46 987887AE Span 8 Pod Liquid System 2 Thread each tubing line through a matching hole in the gasket 3 Push the gasket up the tubing to the position where the tubing emerges from the container through the cap Make sure the gasket is positioned so that the bottom of the tubing reaches the bottom of the container 4 Position the upper tubing retainer approximately 1 2 in 13 mm below the gasket and press each tubing line into a matching groove on the retainer until it is firmly secured 5 Position the lower tubing retainer at the end of the tubing and press each tubing line into a groove on the retainer until it is firmly secured Figure 1 32 The weight of the lower tubing retainer ensures that the tubing ends remain at the bottom of the container Figure 1 3
149. r to main unit Frame the Stacker Carousel according to procedures outlined in CHAPTER 3 Framing Instructions NOTE The Stacker Carousel shuttle must be framed using Teach do not use Auto Teach or Manual Teach IN 987887AE B 31 Using a Stacker Carousel Framing the Stacker Carousel B 32 987887AE a A Absolute Move using in Advanced Manual Control 4 13 Accept using in Hardware Setup 2 3 AccuFrame calibrated by Beckman Coulter Representative 3 2 defined 3 1 installing the 3 6 troubleshooting 3 37 using the 3 6 Add Device using in Hardware Setup 2 2 Advanced Manual Control accessing 4 5 defined 4 1 selection areas for the Span 8 Pod 4 7 verifying liquid level sensing 4 16 ALPs active defined 0 xxix overview 0 xxix passive defined 0 xxix Bar Code Reader aligning laser beam B 23 applying power to B 22 attachment bracket B 22 integrating with Stacker Carousel B 13 positioning labels on microplates B 26 positioning on Stacker Carousel B 16 Biomek NX configurations 0 xxv described 0 xxv Index main components ALPs 0 xxix deck 0 xxix Device Controller 0 xxix heads 0 xxix light curtain 0 xxx pods 0 xxix protective barriers 0 xxx protective side safety shields 0 xxxi C China RoHS Caution Label 0 xi clot detection defined 1 6 how to disable 1 7 performing Find Clot Detection Sensitivities 2 16 sensitivity settings 1 6 tips supported 1 6 troubleshooting
150. re 4 1 appears briefly as the connection is made with the Biomek NX instrument immediately followed by Manual Control Figure 4 2 Figure 4 1 Confirms Manual Control is Connecting Information Es Figure 4 2 Manual Control Manual Control ft Home Z X Move 2 Max Move Gripper Z Max Purge Syringes Set Valve States Get Tip Status Purge System Absolute Move auto Clear G Clear 2 Refresh vector Builder Current Position A X cm cm Span mm cm Z cm Spin Grip cm Back Pod es 812 Re p Gripper P os m T S COSA f B a a a PB Movement Vector Gripper X cm cm Span mm cm Z cm Spin Grip cm a back Te Pod fo fo fo Gripper fo fo fo fo Pa i Vi W2 M3 M4 WS M6 M7 Me aga zw pp ppp pp T Ei D ul fo fo fo fo fo fo fo fo tes za Rotate Left Rotate Ri Jo Xfcm cm Span mm Pod m fon fi ae Speed 100 7 LLS Z Move tcm Z cm Spin Grip cm Gripper fo 1 fo 1 fi fo 1 Using Manual Control The following actions can be performed using Manual Control Homing All Axes of the Span 8 Pod and Gripper Moving a Pod to a Specific Deck Position Stopping a Pod 4 2 987887AE Manually Controlling the Biomek NX in Biomek Software 4 Using Manual Control e Viewing the Firmware Version Accessing Advanced Manual Control Homing All Axes of the Span 8 Pod and Gripper Before selecting OK to home all axes th
151. re Setup Reconnect a Home All Axes Add Device 8 Remove Device J Accept gt lt Cancel Biomek NX SN None Probe Configuration 2 ae AccuFrame Click to select which probes to configure Save Settings Restore Settings Delete Settings 8 Podi Last Validation Not Validated Devices F DeviceControllerO Axis Limit Settings 7 irae X cm Y cm Z cm Dul Span cm ey Shaker lP1 oF StirrerALPO Minimum fz 11 5 5 fis 938 fo fe3 oF StirrerALPL Maximum 102 79 40 fe 56165 ficoo fia 7 47 TiploaderO Probes 1 4 use Set Set Y 7 Tiploadert Disposable x tips amp Digital Devices Ears H E Simulator fim syringes Correlate Pods Gorrelate ZAxes Find LLS Sensitivities OF Camera Disabled Probes OD Materials 1 2 3 4 5 e 7 8 Pump lt None gt x Find GD Sensitivities Stacker Carousels Additional Pod Settings Gripper Settings IV Gripper Installed x cm Y cm Z cm Grip cm offset 7 783 fiz es2 fis4 fo Rotation Calibration Minimum fo fzss1 fo R Maximum 43 47 6 3 fe 19 Set Y Set Grip IV Enable Plate Sensor etect Side Collisions IV Front IV Back I Right IV Left BiomekNX Span 5 Choose Frame Gripper 3 29 3 Framing Instructions Framing the Span 8 Gripper 6 When a Warning appears address the topics that appear in the Warning and
152. regarding the safe and proper disposal of electrical instrumentation The symbol of a crossed out wheeled bin on the product is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates That the device was put on the European Market after August 13 2005 and That the device is not to be disposed via the municipal waste collection system of any member state of the European Union For products under the requirement of WEEE directive please contact your dealer or local Beckman Coulter office for the proper decontamination information and take back program which will facilitate the proper collection treatment recovery recycling and safe disposal of device Chemical and Biological Safety If a hazardous substance such as blood is spilled onto the instrument ALPs or accessories clean up the spill by using a 10 bleach solution or use your laboratory decontamination solution Then follow your laboratory procedure for disposal of hazardous materials If the instrument ALPs or accessories need to be decontaminated contact your Beckman Coulter Representative Risk of chemical injury from bleach To avoid contact with the bleach use barrier protection including protective eyewear gloves and suitable laboratory attire Refer to the Safety Data Sheet for details about chemical exposure before using the chemical viii 98
153. res to protect the Biomek NX operator Safety features of the Biomek NX Span 8 include a front light curtain and Plexiglas side safety shields Figure 5 NOTE An optional side safety shield for device integrations is available Figure 5 Protective Barriers for the Biomek NX Instrument 1 Plexiglass Side Safety Shield 2 Light Curtain Light Curtain Protection System Dark non reflective material affects the sensitivity of the light curtain and adversely impact its effectiveness Typical lab dress such as lab coats and latex gloves do not degrade light curtain operation however it is advisable to test the impact of the all lab dress on light curtain sensitivity before operating the Biomek NX Verify lab dress impact on light curtain sensitivity as follows Use Manual Control in the software and insert the material no more than 1 in past and approximately 21 in above the light curtain panel Make sure the solid amber indicator light changes to blinking amber The diffuse reflective perimeter light curtain along the front edge of the Biomek NX instrument is a standard component NOTE Side safety shields are installed by a Beckman Coulter Representative when the Biomek NX instrument is installed 987887AE Introduction Preventive Maintenance The light curtain projects a diffused array of infrared light rather than a vertical laser like beam Figure 5 When a part of the human body or an object larger
154. rew Up Pump Valve Syringe Base of syringe Thumbscrew ukRWN NOTE Four syringes are configured at a time syringes for probes 1 through 4 and syringes for probes 5 through 8 NOTE Probes 1 through 4 may be configured with one size of syringe and probes 5 through 8 may be configured with another size of syringe Removing and Disassembling Syringes To remove and disassemble syringes 1 Loosen the thumbscrew at the base of the syringe by turning the thumbscrew to the left 987887AE 1 37 Span 8 Pod Pump Assembly 2 Pull the thumbscrew down away from the syringe Figure 1 27 Figure 1 27 Removing Syringes Loosen the Thumbscrew and Pull Down 1 Pump 4 Base of the syringe 2 Valve 5 Thumbscrew 3 Syringe 3 Unscrew the top of the syringe from the valve by turning the syringe to the left 4 Gently pull the syringe down from the valve Figure 1 28 Figure 1 28 Removing Syringes Unscrew and Pull Down on Syringe 1 Pump 4 Syringe 2 Valve 5 Base of the syringe 3 Top of the syringe 6 Thumbscrew 1 38 987887AE Span 8 Pod 1 Pump Assembly 5 Gently pull the plunger out of the glass casing Figure 1 29 Figure 1 29 Removing Syringes Pulling the Syringe Out of the Glass Casing Gam 1 Glass casing 2 Plunger 6 Return the syringe components plunger and glass casing to their original packaging material N Syringe Cutoff Ve
155. rge Pump in Hardware Setup 2 9 Selecting a Purge Pump for a Span 8 Pod 2 10 Hardware Setup Find LLS Sensitivities Button 2 11 Find Sensitivities Start 2 12 Find Sensitivities Start Tip Detection 2 12 Find Sensitivities Load Tips 2 13 Find Sensitivities Tip Configuration 2 13 Find Sensitivities Load Tips Error 2 14 Find Sensitivities Choose Position 2 14 Find Sensitivities Test Sensitivities 2 15 Find Sensitivities Test Sensitivities Indicating Testing Progress 2 15 Find Sensitivities Update Settings 2 16 Hardware Setup Find CD Sensitivities Button 2 17 Find Clot Detection Sensitivities Start 2 18 Find Clot Detection Sensitivities Load Tips 2 19 Find Clot Detection Sensitivities Choose Position 2 20 Find Clot Detection Sensitivities Test Sensitivities 2 21 Find Clot Detection Sensitivities Test Sensitivities Testing Progress 2 21 Find Clot Detection Sensitivities Update Settings 2 22 Hardware Setup Displaying Enable Plate Sensor 2 26 xix Illustrations XX Zag 220 2 29 2 30 231 2 32 3l 3 2 3 3 3 4 35 3 6 ci 3 5 3 9 3 10 ale 3 12 3 13 3 14 3 15 zig 3 17 3 18 3 19 3 20 3 21 Behe 3 23 3 24 3 25 3 26 3 27 3 28 3 29 3 30 Error for No Plate Sensed 2 26 Save Configuration 2 27 Load Settings 2 27 Confirmation to Reset Stored Configuration 2 28 Delete Settings 2 28 Confirmation to Delete Stored Configuration 2 29 AccuFrame Fr
156. ringes Get Tip Status Indicates which probes have tips Purge System Removes air from tubing and purge pump Absolute Move Builds a vector to an absolute coordinate from the current coordinate 9 Current Position Displays the current position of the selected pod 10 Movement Vector Indicates the amount the pod and or probes move when Go is selected 11 Check marks select probes that descend when Go is selected 12 LLS Z Move Verifies liquid level sensing 13 Delta Controls the amount of change applied to the Movement Vector when the Vector Builder is used 14 Vector Builder Used to build a movement vector controls the direction and amount the pod and or probes move These choices change the values in the Movement Vector NOTE Refer to Table 4 1 for further descriptions Table 4 1 Advanced Manual Control Selection Areas for the Span 8 Pod Refer to specific subsections for instructional use Area Description Absolute Move Builds a vector to an absolute coordinate from the current coordinate Active Axes Check marks disable any probes that should not descend when Go is clicked 4 7 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper Table 4 1 Advanced Manual Control Selection Areas for the Span 8 Pod Refer to specific subsections for instructional use Area Description When toggled on each time
157. rument 2 Front of the Biomek NX Instrument 3 The framing shaft is attached to probe 7 to frame positions in the front row of the Biomek NX deck 4 AccuFrame on ALP 5 The framing shaft is attached to probe 1 to frame all deck positions except those in the front row of the Biomek NX deck To prevent damage due to electrical static discharge ESD wear a wrist ground strap when working with tips on the Span 8 Pod To attach the framing shaft to a probe 1 Remove the tip from the desired Span probe probe 1 or 7 refer to CHAPTER 1 Interchangeable Tips 987887AE 3 5 Framing Instructions Framing Deck Positions on the Biomek NX Instrument Using AccuFrame 2 Screw the framing shaft onto the appropriate probe Figure 3 4 Figure 3 4 Attaching a Framing Shaft Details ULL 1 Probes 2 Framing Shaft 3 Install AccuFrame in the position to frame refer to Installing AccuFrame and frame the Span Pod according to the procedure in Framing the Position Installing AccuFrame AccuFrame is used during the framing process to frame deck positions After the framing shaft is attached to the pod and AccuFrame is positioned on the ALP the framing process is completed through the software 3 6 987887AE Framing Instructions 3 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame Turn off power to the Biomek NX instrument before attaching or removing Ac
158. s homes all of the axes for all pods Figure 3 2 Warning to Address Before Homing Process Begin Le xi CAUTION Before selecting OK to home all axes the Following conditions must be met Make sure there is no liquid present in the tips Make sure there are no disposable tips loaded Make sure either disposable tip mandrels or fixed tips ARE installed on the Span 8 Pod Make sure the span gripper is not holding any labware Make sure the span gripper is not near the front back or side of the instrument Cancel 3 Choose OK on each Warning and Information after confirming that the actions have been addressed appropriately a Attaching the Framing Fixture to the Pod 3 4 After homing all axes of the pod the framing shaft is attached to either probe 1 or probe 7 on the Span 8 Pod The framing shaft is attached to probe 1 when all positions except those along the front of the deck are framed Figure 3 3 Because probe 1 is unable to reach the AccuFrame when it is placed in the front deck positions probe 7 must be used to frame positions along the front of the deck NOTE Probes on the Span 8 Pod are numbered from back to front more specifically probe 1 is at the back of the Span Pod and probe 8 is at the front of the pod 987887AE Framing Instructions 3 Framing Deck Positions on the Biomek NX Instrument Using AccuFrame Figure 3 3 Framing Deck Positions with a Span Pod 1 Back of the Biomek NX Inst
159. s of a button 3 Hysteresis Compensation To use the delta value and directional buttons to position the tip 1 In Delta select the magnitude of change applied to the tips each time a directional button is selected Figure 3 18 NOTE The default Delta value is 0 05 cm If the tips are a considerable distance from the desired location increase the distance traveled by increasing the Delta value maximum setting is 1 0 cm If the tips are almost to the desired location reduce the Delta value to fine tune the position minimum setting is 0 005 cm 2 Leave Hysteresis compensation at the default setting of On This compensates for any variance along the X axis from the front of the bridge to the back of the bridge Biomek Software determines the pod s position in the X axis based on the position of the bridge at the back of the Biomek NX instrument 987887AE Framing Instructions 3 Manually Framing Deck Positions on the Biomek NX Instrument 3 select the directional button representing the motion required to physically move the tips into position over the wells of the microplate on top of the ALP Figure 3 18 NOTE Each time a directional button is selected the pod and tips move the distance specified in Delta in the indicated direction NOTE The tips can be physically positioned over the wells of a microplate using e the directional buttons in Manual Teach e the directional keys on the keyboard e the directional keys on t
160. sing a Stacker Carousel B Integrating the Bar Code Reader 7 Using a Phillips head screwdriver loosen laser alignment adjustment screws to adjust laser alignment as necessary Figure B 27 Retighten screws when adjustment is complete Figure B 27 Adjust Laser Alignment as Necessary 1 Laser Alignment Adjustment Screws 2 Laser Beam NOTE The bar code reader is pre configured to read a variety of bar codes 987887AE B 25 Using a Stacker Carousel Configuring the Stacker Carousel in Hardware Setup If labware other than that specified in the Stacker Carousel User s Manual is used an increase in bad reads or no reads may occur If a label is applied by any means other than the Beckman Coulter Print and Apply device an increase in bad reads or no reads may occur 8 If label needs to be applied to the microplate it is recommended that a Beckman Coulter Print and Apply device be used to position the label on the microplate properly Figure B 28 Figure B 28 Label Positioning on Microplate 0 03 0 02 in 0 03 0 02 in 1 45 0 25 in Configuring the Stacker Carousel in Hardware Setup Use Hardware Setup to install configure and remove Stacker Carousels For more information on Hardware Setup refer to CHAPTER 2 Configuring the Biomek NX Span 8 in Biomek Software Hardware Setup is accessed from within Biomek Software Installing Stacker Carousels To install Stacker Carousels 1
161. sing flammable solvents in or near a powered up instrument Observe the appropriate cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials NOTE Observe all warnings and cautions listed for any external devices attached or used during operation of the instrument Refer to applicable external device user s manuals for operating procedures of that device NOTE For Safety Data Sheets SDS MSDS information go to the Beckman Coulter website at www beckmancoulter com 987887AE Safety Notice Moving Parts Moving Parts Risk of personal injury To avoid injury due to moving parts observe the following e Never attempt to exchange labware reagents or tools while the instrument is operating e Never attempt to physically restrict any of the moving components of the instrument e Keep the instrument work area clear to prevent obstruction of the movement Cleaning Observe the cleaning procedures outlined in this user s manual for the instrument Prior to cleaning equipment that has been exposed to hazardous material Contact the appropriate Chemical and Biological Safety personnel e Review the Chemical and Biological Safety information in the user s manual Maintenance Perform only the maintenance described in this manual Maintenance other than that specified in this manual should be performed only by service engineers IMPORTANT It is your responsibility to
162. sition is displayed in Current Position 10 Choose Close to close Advanced Manual Control 987887AE Manually Controlling the Biomek NX in Biomek Software 4 Using Advanced Manual Control with Span 8 Pod and Gripper 11 Choose Exit to close Manual Control N Setting Valve States The valves on the pumps may be set to allow system fluid to flow To and from the syringe with the supply container as the source destination of the fluid Toand from the syringe with tip as the source destination of the fluid Through the valve without activating bypassing the syringe To set the valves on the probes 1 Choose Instrument gt Manual Control Manual Control appears Figure 4 2 2 Choose Advanced Controls 3 Select Pod1 Advanced Manual Control for the pod appears Figure 4 7 4 Choose Set Valve States Valve Settings appears Figure 4 11 Figure 4 11 Valve Settings alve Settings x Valves None Mi M2 M3 M4 M5 ME M7 WB Input Output Purge Done 5 Select the valves by placing a check mark next to the valve s to be set NOTE Choosing All selects all of the valves choosing None selects none 987887AE 4 15 Manually Controlling the Biomek NX in Biomek Software Using Advanced Manual Control with Span 8 Pod and Gripper 6 Choose Input to open the valve and allow system fluid to flow to and from the syringe with the supply container as the source destination of the fluid
163. sitioning Bar Code Reader B 16 Contents Applying Power to the Bar Code Reader B 22 Aligning the Laser Beam B 23 Configuring the Stacker Carousel in Hardware Setup B 26 Installing Stacker Carousels B 26 Configuring Stacker Carousels B 28 Removing Stacker Carousels B 29 Framing the Stacker Carousel B 29 Index Beckman Coulter Inc Warranty and Returned Goods Requirements xvii Illustrations xviii Illustrations 1 1 Span 8 Pod with Factory Installed Gripper Tool on a Biomek NX Instrument 1 2 1 2 Span 8 Pod with Gripper Detailed View 1 3 1 3 Septa Piercing Tip Minimum Liquid Level 1 10 1 4 Fixed Tip Height and Width Restrictions 1 10 is Disposable Tip Height and Width Restrictions 1 11 1 6 Disposable Tip and Tip Shuck Tube Width Restrictions With Test Tubes 1 12 17 Wrist Ground Strap Attached to the Deck 1 14 1 8 Inserting a fixed tip into the tubing 1 16 1 9 Passing Collar Over Fixed Tip 1 17 1 10 Installed Fixed Tip 1 18 Tii Fully Installed Fixed Tip 1 20 1 12 Pulling the Collar off the Fixed Tip 1 21 i Removing a Fixed Tip 1 22 1 14 Inserting a Disposable Tip Mandrel into the Tubing 1 23 115 Passing Collar Over a Disposable Tip Mandrel 1 24 1 16 Installing a Tip Shuck on a Disposable Tip Mandrel 1 25 i Installed Disposable Tip Mandrel 1 26 1 18 Fully Installed Disposable Tip Mandrel 1 28 1 19 Removing a Tip Shuck Tube From a Disposable Tip Mandrel 1 29 1 20 Removing the Coll
164. ssary refer to the Stacker Carousel User s Manual PN 148598 for instructions on how to adjust the height of the stacker Attaching the Side Shield Attach the side shield as follows 1 orient the Plexiglas side shield to enable the shuttle to access the side shield cutout without obstruction Figure B 7 through Figure B 9 NOTE Following this diagram in Figure B 7 attach the lower side shield components to the top side shield Use the middle side shield component for both the front and back mount orientations 2 Following this diagram in Figure B 7 attach the lower side shield components to the top side shield B 8 987887AE 987887AE Using a Stacker Carousel B Integrating the Stacker Carousel 3 Attach the shield to the Biomek NX using the screws provided NOTE Use the same attachment procedure for either side of the instrument Figure B 7 Attaching the Side Shield Components to Biomek NX 1 Screws included with side shield integration kit 4 Middle side shield 2 Screws included with ship kit 5 Front back side shield 3 Screws included with side shield integration kit 6 Top side shield B 9 Using a Stacker Carousel Integrating the Stacker Carousel 4 Align the shuttle to load and dispense labware through the Plexiglas side panel opening Figure B 8 and Figure B 9 Figure B 8 Aligning the Stacker Carousel Shuttle B 10 987887AE 987887AE Biomek
165. st current information bulletins 987887AE Safety Notice Electrical Safety concerning the product If you purchased this product from a third party and would like further information concerning this topic contact your Beckman Coulter Representative Electrical Safety To prevent electrically related injuries and property damage properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies Contact a Beckman Coulter Representative for any servicing of equipment requiring the removal of covers or panels High Voltage This symbol indicates the potential of an electrical shock hazard existing from a high voltage source and that all safety instructions should be read and understood before proceeding with the installation maintenance and servicing of all modules Do not remove system covers To avoid electrical shock use supplied power cords only and connect to properly grounded three holed outlets Laser Light This symbol indicates that a potential hazard to personal safety exists from a laser source When this symbol is displayed in this manual pay special attention to the specific safety information associated with the symbol Laser Specifications Laser Type Class II Laser Diode e Maximum Output 11 mW e Wavelength 670 nm 987887AE vii Safety Notice Chemical and Biological Safety Disposal of Electronic Equipment It is important to understand and follow all laws
166. stay on when no objects are breaking the light beams There is most likely an internal obstruction to sensors Call a Beckman Coulter Representative 3 37 Framing Instructions Troubleshooting 3 38 987887AE CHAPTER 4 Manually Controlling the Biomek NX in Biomek Software Overview Manual Control and Advanced Manual Control are used to control Movement of the pod and gripper independently of a method NOTE The gripper is a factory installed optional and may not be installed later Pod when teaching the deck framing the gripper and recovering from errors ALPs and Device Controllers independently of a method NOTE Refer to the ALPs User s Manual PN 987836 for information on manually controlling specific ALPs The sections in this chapter include e Accessing Manual Control e Using Manual Control e Using Advanced Manual Control with Span 8 Pod and Gripper Accessing Manual Control Manual Control is available only when a method is not being executed If a need for manual control is realized during a method run stop the method using the Stop button or Snap Continuation button refer to the Biomek Software User s Manual PN 987835 Snapping a Continuation on the toolbar before accessing Manual Control 987887AE 4 1 Manually Controlling the Biomek NX in Biomek Software Using Manual Control To open Manual Control e Choose Instrument gt Manual Control An Information dialog Figu
167. strument overhangs the table work surface on either side a protective bottom shield is also installed on the side of the instrument overhanging the instrument envelope Preventive Maintenance To ensure optimum operation of the Biomek NX perform the following maintenance procedures as necessary 987887AE xxxi Introduction Troubleshooting To prevent injury use proper decontamination procedures Wipe up all spills on the deck immediately Approximately every six to twelve months schedule a Beckman Coulter service call to lubricate the linear rail Light Curtain Maintenance To ensure optimum operation of the light curtain protective system perform the following maintenance procedures as necessary Once a week verify proper operation of light curtain using Manual Control in the software refer to CHAPTER 4 Manually Controlling the Biomek NX in Biomek Software and the light curtain test rod supplied with Biomek NX Insert the test rod no more than 1 in past and approximately 21 in above the front light curtain panel Check to make sure the solid amber indicator light changes to blinking amber each time the light curtain is violated If not contact your Beckman Coulter Representative When necessary clean light curtain panels with a lint free cloth Once every 2 3 months clean light curtain panels with a non abrasive cleaner making sure not to scratch the strip Troubleshooting If service is required cont
168. t contacting a Beckman Coulter Representative Set Grip Configures the gripper motion settings Do not change the axes limits without contacting a Beckman Coulter Representative Set Y Establishes the distance the gripper can move along the Y axis Do not change the axes limits without contacting a Beckman Coulter Representative Rotation Calibration Centers the gripper to ensure correct gripper rotation Using the Plate Sensor A plate sensor on the optional gripper tool is available When Enable Plate Sensor Figure 2 26 is checked in Hardware Setup and plate sensor for the specific labware in the Labware Type Editor is checked labware can be sensed when the gripper fingers are squeezed An error message Figure 2 27 appears if labware is not sensed when the gripper fingers are squeezed Refer to the Biomek Software User s Manual PN 987835 Handling and Preventing Errors for information on recovering from errors NOTE By default Enable Plate Sensor is enabled 2 25 2 Configuring the Biomek NX Span 8 in Biomek Software Saving Restoring and Deleting Settings Figure 2 26 Hardware Setup Displaying Enable Plate Sensor B Reconnect ff Home All axes q Add Device s Renove Device Y Accept gt Cancel Gl Biomek NX SN None Probe Configuration 2 Z p f AccuFrame Click to select which probes to configure Save Settings Restore Se
169. tact moving parts You mishandle broken parts Doors covers and panels are not opened closed removed and or replaced with care Improper tools are used for troubleshooting To avoid injury Keep doors covers and panels closed and secured in place while the instrument in use Take full advantage of the safety features of the instrument Do not defeat safety interlocks and sensors Acknowledge and act upon instrument alarms and error messages Keep away from moving parts Report any broken parts to your Beckman Coulter Representative Use the proper tools when troubleshooting System integrity could be compromised and operational failures could occur if This equipment is used in a manner other than specified Operate the instrument as instructed in the Product Manuals You introduce software that is not authorized by Beckman Coulter into your computer Only operate your system s computer with software authorized by Beckman Coulter You install software that is not an original copyrighted version Only use software that is an original copyrighted version to prevent virus contamination If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter distributor and if it is not presently under a Beckman Coulter Service Maintenance Agreement Beckman Coulter cannot guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the mo
170. tainer for 24 to 48 hours prior to attaching it to the Biomek NX Span 8 instrument refer to Supply Container The lack of motion allows the air bubbles in the system fluid to escape or burst prior to use NOTE It is recommended that extra supply containers be degassed at all times so that properly degassed system fluid is readily available when a supply container must be replaced refer to Supply Container Priming the System with System Fluid Priming the system is the initial aspiration of system fluid into the tubing for the Span 8 Pod It ensures that system fluid is in all the tubing prior to purging the system of air and performing any pipetting actions Priming the system with system fluid is accomplished through Manual Control refer to Purging Air from the Syringes and Tubing 1 51 1 Span 8 Pod Purge Pump Purging the Tubing and Syringes of Air Do not purge the system without mandrels installed and tubing attached to disposable or fixed tips To purge the system without the mandrels installed and the tubing attached to tips may cause corrosion in the tip interface To accurately transfer liquid using the Span 8 Pod air must be purged from the tubing and syringes prior to running a method The tubing and syringes must also be purged of air after new tips or syringes are installed The purging process draws system fluid through the tubing and syringes until all air bubbles are removed from the tubing and syringes Purging t
171. te Tubing 4 Span 8 Tip Wash ALP 2 Cap Waste Tubing 3 Gasket 5 Tubing Retainer 2 Thread each tubing line through the matching hole in the gasket 1 50 987887AE Span 8 Pod Liquid System 3 Push the gasket up the tubing to the position where the tubing emerges from the container through the cap 4 Position the tubing retainer approximately 1 2 in 13 mm below the gasket and press each tubing line into the matching groove on the retainer until it is firmly secured Preparing the Liquid System 987887AE Air must be removed from the system fluid and tubing to maximize the pipetting performance of the Span 8 Pod Air removal is accomplished by Degassing the System Fluid e Priming the System with System Fluid e Purging the Tubing and Syringes of Air Degassing the System Fluid The system fluid must be degassed prior to use Degassing the system fluid improves pipetting accuracy by reducing the amount of air in the system fluid The system fluid is compressed and released in the syringes to create a vacuum used during pipetting operations however air in the system fluid compresses at a different rate than the system fluid itself which can cause pipetting errors To reduce pipetting errors caused by air in the system fluid the system fluid must be degassed prior to priming the Biomek NX Span 8 instrument with the system fluid Degassing the system fluid is accomplished by letting the system fluid rest in the supply con
172. tection Sensitivities Test Sensitivities Find Clot Detection Sensitivities Start Testing Progress Load Tips Initializing device for sensitivity tests Choose Position Test Sensitivities Update Settings Figure 2 24 Find Clot Detection Sensitivities Test Sensitivities Testing Progress Find Clot Detection Sensitivities Start z Testing Progress Load Tips Determining maximum sensitivities for each probe Choose Position Test Sensitivities Update Settings 987887AE 2 21 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Do not choose Cancel if testing is stopped before the probe sensitivities have been completed clot detection will not function and Find Clot Detection Sensitivities must be repeated It takes nearly 30 minutes to complete the testing 11 when testing is completed choose Next Find Clot Detection Sensitivities Update Settings appears indicating the probe sensitivities have been completed Figure 2 25 Figure 2 25 Find Clot Detection Sensitivities Update Settings Find Clot Detection Sensitivities Start Updating Probe Sensitivities Completed Load Tips Probe 1 4400 Updated Choose Position Probe 2 4850 Updated Test Sensitivities Probe 3 4500 Updated Probe 4 4550 Updated Probe 5 5050 Updated Probe 6 4600 Updated Probe 7 4750 Updated Probe 8 4200 Updated Update Settings Cancel
173. than approximately 1 in in diameter such as labware and large cables penetrates this protective zone the instrument shuts down immediately stopping all pod and head operations Some ALP operations such as shaking or stirring continue ALPs respond to a violation according to safety and operational requirements specific to each ALP For example a refilling reservoir may continue to operate if user safety is not compromised ALPs that operate with a motion that could present a danger to the operator go to a safe state when the light curtain is violated NOTE When active ALPs or optional devices are operating and the light curtain is violated an error message may not appear until after the ALP or optional device operation is complete NOTE It is important to become familiar with this protected zone This reduces the possibility of causing the instrument to shut down accidently during operation by unintentionally violating the light curtain zone When the instrument is sitting idle or in the paused mode no violations are registered when the protective zone is penetrated This allows full access to instrument components ALPs and labware on the Biomek NX deck during a pause or system idle time Side Safety Shields To reduce the risk of personal injury operate the Biomek NX instrument only with all protective shields in place Side safety shields are installed by the Beckman Coulter Representative on the Biomek NX instrument If the in
174. the plate does not move when lifted then click Finish Wearing I Technique Delta 0 05 cm u Setup 74 Back Left Bight Hysteresis compensation Total Moved From Start cm Ek ee TeachZ Finish cont Le 1 In Delta select the magnitude of change applied to the pod each time a directional button is selected Figure 3 19 NOTE The default Delta value is 0 05 cm If the tip is a considerable distance above the position increase the distance traveled by increasing the Delta value maximum setting is 1 0 cm If the tip is almost to the desired location reduce the Delta value minimum setting is 0 005 cm NOTE Since the X and Y axes were framed previously it is safe to move the pod in the X and Y axes if it makes framing the pod in the Z axis easier 2 Select the directional button representing the motion required to physically move the pod down until the tip just touches the microplate at the well corner or the bottom of the well NOTE Each time a directional button is selected the pod and tips move the distance specified in Delta in the indicated direction NOTE The tip can be can physically positioned over the labware positioner using e the directional buttons in Manual Teach e the directional keys on the keyboard e the directional keys on the numeric keypad The directional buttons displayed in Manual Teach parallel the keys on the numeric keypad More specifically Fwd
175. the system hierarchy displayed in the left pane of the Hardware Setup The Span Pod configuration appears in the right pane Select a Purge Pump for the Span 8 pod Figure 2 8 NOTE A purge pump expels system fluid through every probe on the Span 8 Pod not selected or individual probes 2 9 Configuring the Biomek NX Span 8 in Biomek Software Configuring the Span 8 Pod Figure 2 8 Selecting a Purge Pump for a Span 8 Pod Biomek Hardware Setup Reconnect Mf Home All Axes Gp Add Device s Re ce J Accept gt lt Cancel El Biomek NX SN None Probe Configuration j i EOE Delete Settings E AccuFrame Click to select which probes to configure 730e Settings estore settings pee eh a Pod1 Last Validation Not Validated Set Validation Time a Devices T DeviceControllerd Axis Limit Settings T PurgePumpo x cm Y cm Z cm D uL Span cm 4 seen minimum F211 fss fiss fo fos ET SourceWasteSensor0 maximum 102 79 40 12 13815 1000 jia of StirrerALPO Probes 1 4 use Set X Set Y of StirrerALP1 xea z ti L ips ST Tiploadero gt PRI AT Tiploadert 100 pl w syringes Correlate Z Axes Find LLS Sensitivities A Digital Devices Disabled Probes EH Simulator 1 2 3 4 5 6 7 8 Pum roe Find CD Sensitivities E Camera E Materials A E Stacker Carousels Additional Pod Settings Stacker1 a Speed Limit 100 Additional Timeout 250s Unload Speed 25 Probe
176. to 1025 pL liquid and air capacity NOTE Refer to Interchangeable Tips for more information on tips available for use with the Span 8 Pod Disabling Clot Detection If clot detection is disabled in the Technique governing the liquid handling procedure clot detection is unavailable regardless of the type of tips attached to the probes Refer to the Biomek Software User s Manual PN 987835 Setting Technique Values for enabling and using clot detection in techniques Selecting Which Type of Tips to Use 987887AE The best tips to use depends on a number of factors relating to the application such as the liquid and labware types used and volumes required for aspirate and dispense operations There are 13 types and sizes of fixed and disposable tips that can be attached to the probes These tips are listed in Table 1 2 Table 1 2 Span 8 Tip Types Tip Name LLS CD Tp Type Software Capable Tip Volume Fixed 60 Coated maximum of 5000 uL liquid and or air Fixed60 Yes caD Fixed 60 Uncoated capacity depending on syringe size Fixed 100 Coated maximum of 5000 uL liquid and or air Fixed100 Yes ab Fixed 100 Uncoated capacity depending on syringe size i f L liqui i Septa Piercing Fixed SeptaFluted Yes peck i aes a 7 capacity depending on syringe size Disposable 20uL LLS AP96_20_LLS Yes maximum of 100 uL liquid capacity up to 120 uL air capacity depending Disposa
177. ttings a Delete Settings u 8 odii Last Validation Not validated Set Validation Time o Devices 7 DeviceControllerO Axis Limit Settings 7 PurgePumpO x cm cm 2 cm D ul Span cm a oie Minimum 2 11 fss fas s3a fo fos g7 aKEr oF SourceWasteSensor0 Maximum 102 79 40 AEE 1000 jia7 oF StirrerALPO Probes 1 4 use Set x Set Y SF StirrerALP1 Fredo gt tes Eee eal amp Digital Devices ee Bg Simulator 1ml syringes Correlate Pods Correlate Z Axes Find LLS Sensitivities E Camera Disabled Probes E Materials 1 2 3 4 5 6 7 8 Pump lt None gt x Find CD Sensitivities Stacker Carousels meeeseze Y Additional Pod Settings Gripper Settings IV Gripper Installed Xem Y cm Z cm Grip cm ect Side Collisions offset 7 783 o 7 32 f Rotation Calibration M Front I Back Minimum 0 29 81 o Rees Maximum 43 47 6 3 6 19 I Right Set Y Set Grip Enable Plate Sensor M Left BiomekNX Span 1 Enable Plate Sensor Figure 2 27 Error for No Plate Sensed Biomek Software Error in Move Labware from P3 to Orbital Eror during Assert labware gripped There was no plate sensed by the grippers Betry Snap 2 7 2004 11 05 22 AM Saving Restoring and Deleting Settings The axes limits and pod settings may be saved restored and deleted using the Save Settings Restore Settings and Delete Settings options in Hardware Setup Saving Pod Settings To save settings 1 In Hardware Set
178. uFrame 3 8 1 InBiomek Software choose Instrument gt Deck Editor Deck Editor appears Figure 3 5 Figure 3 5 Deck Editor Deck1 Default Deck x if E it y New Deck Delete Deck Rename Deck OpenDeck ClearDeck Renumber Delete ALP Properties Save Cancel PaositivePosition amp LP ShakerALP SpansactiveWashLefl SpansactiveWashRigl Span8TipTrash Span8TrashLeft i 7 Coords cm eb aI eb j 2 InBiomek Software open the deck that requires framing in the Deck Editor Verify that it reflects the current configuration of ALPs on the physical deck If it does not reflect the current physical deck configuration place devices on the appropriate deck locations in the Deck Editor by dragging and dropping from the list onto the deck When the Deck Editor reflects the configuration of the current physical deck proceed to step 3 3 Double click on the deck position containing the AccuFrame Position Properties appears Figure 3 6 NOTE The coordinates displayed are default values the software must be taught precisely where the position is on the physical deck Figure 3 6 Position Properties Name fa ALP Type FourByThreeHD X cm cm Z cm Precision Pod1 Coordinates e 645 13 436 16 4 Not Framed Advanced MC Teach More gt gt Manual Teach Auto Teach Lx J ea 987887AE Framing Instructions 3 Framing Deck Positions on the Biomek NX Instrument Usin
179. uid Level Sensing Settings Sensitivity settings for liquid level sensing affect the magnitude of change required for LLS tips to detect the liquid A higher sensitivity setting detects smaller changes in the capacitance detected refer to the Biomek Software User s Manual PN 987835 Setting Technique Values Disabling Liquid Level Sensing If LLS is disabled in the Technique governing the liquid handling procedure LLS is unavailable regardless of the type of tips attached to the probes Refer to the Biomek Software User s Manual PN 987835 Setting Technique Values for enabling and using liquid level sensing in techniques 1 Span 8 Pod Interchangeable Tips Clot Detection When working with blood samples observe the cautionary procedures as defined by your safety officer The Span 8 Pod can detect if a possible clot is on the end of a tip after an aspirate operation when conductive tips called LLS in the Tip Type Editor are attached to the probes NOTE Conductive tips must be attached to the probes to enable clot detection NOTE vAn LLS plate option used on a 1x1 Passive ALP can improve clot detection capabilities refer to the ALPs User s Manual PN 987836 1x1 Passive ALP with LLS Plate Option Conductive tips fixed disposable or septa fluted are used to determine if a clot is detected by detecting a shift in the capacitance The tip moves to a specified height within the well and then slowly moves down into th
180. ult Delta value is 0 05 cm NOTE If the microplate is a considerable distance from the desired location increase the distance traveled by increasing the Delta value maximum setting is 1 0 cm If the microplate is almost to the desired location reduce the Delta value to fine tune the position minimum setting is 0 005 cm Select the directional button representing the motion required to physically move the microplate into the appropriate position at the position being framed Figure 3 18 NOTE Each time a directional button is selected the gripper and microplate move the distance specified in Delta in the indicated direction NOTE The microplate can be physically positioned over the position being framed using e the directional buttons in Manual Teach e the directional keys on the keyboard e the directional keys on the numeric keypad The directional buttons displayed in Manual Teach correspond to the keys on the numeric keypad More specifically Fwd corresponds to the 1 on the numeric keypad while Down is found on the 2 Left is found on the 4 Right on 6 Up on 8 and Back on 9 Visually verify the position of the microplate in relation to the position being framed If the microplate is still not accurately seated in the position being framed repeat steps 1 and 2 until it is accurately positioned in the position being framed NOTE The microplate may also be aligned in the X and Y axes using the
181. up select the Span 8 Pod from the left pane to access the configuration area in the right pane 2 Choose Save Settings Save Configuration appears Figure 2 28 2 26 Configuring the Biomek NX Span 8 in Biomek Software 2 Saving Restoring and Deleting Settings Figure 2 28 Save Configuration Save Configuration Name Settingst Description 3 Create a name for the current configuration 4 if desired enter a description of the configuration g1 Choose OK The settings are saved Restoring Pod Settings To restore the settings 1 In Hardware Setup select the Span 8 Pod from the left pane to access the configuration area in the right pane 2 Choose Restore Settings Load Settings appears Figure 2 29 Figure 2 29 Load Settings Settings Settings Information Validation Time Not Validated Pump lt None gt Probe Configurations 1 Fixed100 tips 1 mL syringes 2 Fixed100 tips 1 mL syringes 3 Fixed100 tips 1 mL syringes 4 Fixed100 tips 1 mL syringes 5 Fixed100 tips 1 mL syringes 6 Fixed100 tips 1 mL syringes 7 Fixed100 tips 1 mL syringes 8 Fixed100 tips 1 mL syringes Axis Limit Settings X 211 102 79 Y 5 50 40 00 Span 6 30 14 70 xl cea 987887AE 2 27 Configuring the Biomek NX Span 8 in Biomek Software Saving Restoring and Deleting Settings 3 Select the desired settings 4 Choose OK The following Confirm appears
182. with a gripper tool Figure 1 1 that grasps and moves labware from one location on the Biomek NX deck to another The gripper is a factory installed option and may not be installed later 987887AE Span 8 Pod 1 Gripper The gripper tool Figure 1 35 moves independently of the pod and can move in the Y axis back to front and Z axis up and down It can also rotate to access positions that are oriented at different angles in relation to the front of the Biomek NX instrument however controlling this feature in Biomek Software in a method is available only for custom integrations The gripper can e move any stack of labware 6 5 cm 2 56 in or smaller NOTE Astack of four standard height 96 well microplates is approximately 5 2 cm 2 05 in high NOTE The gripper tool stacks and unstacks groups of labware from the bottom If a stack containing four pieces of labware is being unstacked the gripper grasps the top three and moves them to a new position Then the gripper grasps the top two of that stack moves them finally the gripper grasps the top piece of labware in that stack and moves it to a new location Refer to the Biomek Software User s Manual Specifying Stackable Items and Using Secure Stacking for more information on using labware stacks with the Biomek NX Span 8 gripper e place lids on and remove lids from labware Figure 1 35 Factory Installed Gripper Tool 1 Single finger pad 2 Doub
183. without causing any damage to the tips probes pod or ALP Manual Teach can also be used to frame positions that cannot be accessed by the probes using the gripper if installed NOTE Some ALPs such as the Positive Position ALP must be framed using Manual Teach to improve pipetting accuracy to high density labware NOTE When not using high density labware the standard framing procedure using the AccuFrame is acceptable To frame using the AccuFrame refer to Framing Deck Positions on the Biomek NX Instrument Using AccuFrame To frame using Manual Teach 1 Choose Start gt Programs gt Beckman Coulter gt Biomek to open Biomek Software 2 From the Instrument menu choose Deck Editor Deck Editor appears Figure 3 9 Figure 3 9 Deck Editor Deck1 Default Deck E x t E it New Deck Delete Deck Rename Deck OpenDeck ClearDeck Renumber Delete ALP Properties Save Cancel PositivePosition amp LP ShakerALP SpandactiveWashLefl SpansActiveWashRigl Span8TipTrash Span8TrashLeft i H Coords cm fo fo fo 987887AE 3 11 Framing Instructions Manually Framing Deck Positions on the Biomek NX Instrument 3 4 5 3 12 Open Position Properties for the desired deck position by double clicking on the deck position Position Properties appears Figure 3 10 Figure 3 10 Position Properties for a Positive Position ALP Position Properties Name J ALP
184. y Description Detect Side Collisions Front Back Right Left Enables the notification of potential collisions between the gripper and side shields Enable Plate Sensor Enables the plate sensor on the gripper to sense labware Default setting is checked enabled Refer to Using the Plate Sensor Frame Gripper Frames the gripper providing a point of reference for all gripper movements along the X Y and Z axes Do not reframe the gripper without contacting a Beckman Coulter Representative Gripper Installed This check box defaults as checked if an optional gripper tool is installed and enables the configuration of gripper settings Gripper X Y Z Grip Offsets Controls gripping operation along X Y and Z axes from center of back edge of deck position These offsets are automatically updated when the gripper is framed NOTE Gripper X and Z offsets are read only Maximum X Y Z and Grip The maximum position the gripper may go to along the X Y and Z axes relative to the Home position Set by using the appropriate buttons under the X cm and Y cm columns Do not change the axes limits without contacting a Beckman Coulter Representative Minimum X Y Z and Grip The minimum position the gripper may go to along the X Y and Z axes relative to the Home position Set by using the appropriate buttons under the X cm and Y cm columns Do not change the axes limits withou
185. ze The size of a syringe dictates the maximum amount of fluid aspirated into and dispensed from the tubing for each of the probes and the speed at which the fluid flows Syringes are available in six volumes e 100 uL e 250 uL e 500 uL e iml e 25mL e 5mL NOTE A syringe reflecting the maximum volume most frequently aspirated and dispensed during a method should be installed in the pumps Two different sizes of syringes can be used in the pump assembly at the same time in order to perform pipetting operations at different volumes during a single method NOTE Probes 1 through 4 must all use the same size syringe and probes 5 through 8 must all use the same size If two different sizes of syringes are required to ensure pipetting accuracy within a single method or the size of syringes installed in the Biomek NX Span 8 instrument changes frequently new techniques should be created for each size of syringe This is accomplished by copying the Span 8 techniques to create multiple sets of techniques and by editing the Cutoff Velocity and other pipetting parameters in those techniques to support the size of syringe s installed NOTE Refer to the Biomek Software User s Manual PN 987835 Modifying Saved Techniques for more information on copying techniques and Overriding Liquid Type Values for more information on editing liquid types Assembling and Installing Syringes Syringes are shipped in two pieces Plunger e Glass casing

Biomek NX and NXP Laboratory Automation

Contents

Download Pdf Manuals

Related Search

Related Contents