SC-type Piston Pump Heads User`s Guide
Contents
1. Additional Accessories 2 For any Liquid For Any Liquid It is recommended to place this filter upstream from the injector in any HPLC system This efficient filter provides a final polish to the mobile phase and a durable protection for the injection valve Part Number Description High pressure in line filter 316L 0 5 um 2 5 cm 0 4 ml 4970050105 100 MPa ssi 05 0105 29410652 Replacement filter 316L 0 5 for high pressure in line filter 4970050105 551 05 0106 Additional Accessories For Water Aqueous Solutions and Polar Solvents These HDPE high density polyethylene piston seals are yellow to be easily distinguished from the black PTFE seals PTFE black seals are recommended with alkanes chlorinated solvents tetra hydrofuran and carbon dioxide HDPE yellow seals give longer service with water aqueous solutions alcohols and acetonitrile Part Number Description 5463088863 HDPE piston seal for 5SC head 5463125895 HDPE piston seal for 10SC and 10WSC heads 5463050825 HDPE piston seal for 25SC head 5463070820 HDPE piston seal for 50SC head 5463394862 HDPE piston seal for 100SC head 1 JeJod pue suonnjos snoanby 104 Additional Accessories 2 For High viscosity or High density Liquids For High Viscosity or High Density Liquids This spring loaded check valve is specially designed for pumping highly viscous or dense liquids Refer to Chapter2. check valve screw for 1 8 SSI fitting two SSI 1 8 screws and ferrules and 316L tubing 2000 x 3 2 x 2 1 mm 3650220 Metallic inlet fitting for 505 100SC and 200WTi pump heads Only consists of inlet check valve screw with Swagelok fitting 1 4 316L Tubing not supplied 1uaiquie uow spinbr 104 Additional Accessories 2 For Liquids at Non ambient Temperatures 10 Part Number Description 5463125795 Piston seal PTFE graphite for heads 10SC 10WSC and 10WTi 360358311 Fitting for European gas cylinder 5 8 BSP 1 8 SSI 360358314 Spare seal for fitting of European gas cylinder FEP 10 5 x 2 7 mm 5 units Fitting for American gas cylinder 0 5 14 NPSH 1 8 SSI 21 02 51 30 Spare Seal for fitting of American gas cylinder one unit 49 03 17 04 Additional Accessories For Heads 50SC and 100SC These items answer the need to extend the length of the inlet tubing up to three meters so that a large capacity reservoir located on the floor can feed one or more preparative workstations Part Number Description 3650075 Inlet tubing FEP 3 x 4 x 3000 mm M10 and 20 um filter for 50SC and 100SC F1410156 Union fitting PVDF M10 25001 pue 9506 SpeaH 104 11 Additional Accessories 12 Description 3 Outlet Outlet check valve support screw Outlet check valve carriage Piston seal Anti extrusion gasket Housi
3. hydrocarbons or chlorinated volatile solvents Flowrate Accuracy At 20 and over full working range maximum accuracy error with water is for 302 and 303 0 to 2 5 up to 10 MPa for 305 and 306 1 up to 60 MPa List of Replacement Parts 8 Part Number Description 5SC 10SC 25SC 50SC 100SC 360510 360520 360522 360524 360530 Piston seal PTFE 5463088063 5463125095 5463050025 5463070020 5463394062 HDPE 5463088863 5463125895 5463050825 5463070820 5463394862 Anti extrusion 3650014K 3650015 36500160 3650017 3650018K Inlet check valve 36501701 36501701 36501701 3650263 3650263 cartridge Outlet check valve 36501801 36501801 36501801 3650267 3650267 cartridge Piston assembly 3650008 3650009 3650011 3650012 3650013 Spring spacer 3650019 3650019 3650019 3650020 3650020 Piston tail guide 3650027 3650027 3650027 3650028 3650028 Return spring 36300058 36300058 36300058 36300062 36300062 Ball housing 3650067 3650067 3650067 3650068 3650068 assembly 39 Trademarks Hastelloy is a registered trademark of Haynes Elgiloy is a registered trademark of Elgiloy Co 40 World Wide Web wwwgilson com E mail sales gilson com service gilson com training gilson com World Headquarters Gilson Inc 3000 Parmenter Street P O Box 620027 Middleton WI 53562 0027 USA Telephone 1 800 445 7661 or 1 608 836 1551 e Fax 1 608 831 4451 Gilson SAS 19 avenue des Entrep
4. 5 Viscosity It consists of two spring loaded check valve cartridges to be substituted for the standard cartridges plus a silicone rubber inlet tubing 2000 x 5 x 3 mm and a male luer fitting to be substituted for the standard inlet tubing assembly This kit is capable of being used without an inlet filter Description Spring loaded check valve kit PCTFE envelope for 5SC 10SC 25SC and 10WSC heads Additional Accessories For Liquids at Non Ambient Temperatures Gilson pump heads operate from 20 C up to 100 C provided that the liquid pumped including liquefied gases and melted solids is chemically compatible with the materials see Technical Data The two following thermostatic kits are available for heating or cooling Gilson pump heads Both require a standard cryothermostat not supplied by Gilson that generates a temperature controlled circulating liquid Part Number Description 360350400 Thermostatic jacket kit for any model of Gilson piston pump heads Requires metallic inlet see below 3650205 or 3650220 according to pump head model 3650430 Complete thermostatic kit for 55 10SC and 25SC pump heads Includes all required components jacket other elements of the thermostating loop and full thermostated line These kits have their own additional accessories Part Number 3650205 Description Metallic inlet kit for 55 10SC 25SC and 10WSC pump heads Consists of inlet
5. N 2 TETRAHYDROFURAN BUTYL ALCOHOL CHLOROFORM 1 CYCLOHEXANE DICHLOROETHANE 1 DICHLOROMETHANE DI ETHYLETHER DI PROPYLETHER TOLUENE ACETIC ACID ACETONE ACETONITRILE ETHYLACETATE ETHANOL HEPTANE HEXANE METHANOL 1 ISOPROPANOL WATER XYLENE wisciBLE B miscele Precautions for Use Changing the Flowrate 22 Changing the Flowrate To protect the HPLC columns the flowrate should be changed in progressive steps never abruptly Precautions for Use Salinity and Acidity This section justifies the Gilson offer of three types of pump head SC WSC and WTi The figure on the next page shows the typical working range of each type according to salinity and acidity at ambient temperatures Special precautions to take when using SC type pump heads In order to avoid any possible deposit of solid crystal inside SC type pump heads when pumping saline solutions e g buffer salts or ion pairing salts included in chromatographic mobile phases At the end of the day run the pump at a very low flowrate 10 l min At the end of the run rinse the head with water to remove any salt which may have precipitated With a WSC or WTi type pump head this precaution is not necessary In the particular case of solutions which contain halide ions e g chloride in addition to taking the above precautions it is recommended to keep the con centration and the pH wit
6. SC type Piston Pump Heads User s Guide LT801077R 2006 Gilson SAS All rights reserved February 2006 Table of Contents Unpacking and Standard Accessories Additional Accessories For Any Liquid For Water and Aqueous Solutions For High Viscosity or High Density liquids 8 For Liquids at non ambient Temperatures 9 For Heads 50SC and 1005 Description Setup Tubing Connections Precautions for Use Solvent Filtration Use New Pump Head New Piston Seal 19 Solvent Degassing Solvent Miscibility Changing the Flowrate Salinity and Acidity Viscosity Temperature 6 Maintenance Check Valves Piston Seal Return Spring Maintenance Kit 7 Technical Data Liquid Flow Data 8 List of Replacement Parts Unpacking and Standard Accessories 1 The Gilson SC type Piston Pump Head series consists of five models 5SC 10SC 25SC 50SC and 100SC Each one is supplied complete in a kit Figure below with the standard accessories as listed in Table overleaf Each pump head is filled with isopropanol when delivered Unpack the kit and compare the contents with your pur chase order and Table I to verify that all parts are included and undamaged User s Guide Inlet tubing assembly FEP additional PVDF coupling inlet tubing 1 4 28TPI S S outlet tubing PTFE outlet tubing 250mm Allen wrench 3mm SSI screw Piston seal SSI ferrule mounting tool 10 ml syringe Washer PVDF coupling 1 4 28TPI U
7. absorbance detector working at low wavelength at 210 nm a 1 change in dissolved oxygen level will cause a 4 x 10 A U change in the absorbance of methanol Precautions for Use Solvent Miscibility When changing solvents make sure that the new solvent is miscible with the previous one Otherwise use an intermediate solvent Benmac ACETONE ACETONITRILE BUTYL ALCOHOL CHLOROFORM a CYCLOHEXANE DICHLOROETHANE m DICHLOROMETHANE DIMETHY FORMAMIDE DIMETHYL SULFOXIDE DIOXAN o ETHYLACETATE E ETHANOL O HEPTANE HEXANE METHANOL i METHYLETHYL KETONE FOCTANE ISOPROPANOL DI PROPYLETHER TETRAHYDROFURAN TOLUENE WATER 1 Dichloromethane 2 Tetrahydrofuran 3 Ethanol H H H H 8 OO n LIB LIE CT CT HH H H99M DBpmgladgiiuuuum upgiosiy 1u9AjJoS LIBE CLE ELE EJ CT OB 8oO0o000 Ao Oo o oao OEO Oo XYLENE DIMETHYLFORMAMIDE IBI OOOO IBI C E EB BE EC CDI ET ECT EI CJ METHYLETHYL KETONE O IBI C CI E E EI E 1 CI DIMETHYL SULFOXIDE DIOXA
8. bnormal loss in flowrate The check valves must not be disas sembled into sub components No check valve sub component is available from Gilson Check valve cleaning e Pump isopropanol if it is miscible with the current solvent Otherwise pump an intermediate solvent first When the pump head is filled with isopro panol stop the flow for 15 minutes or more to dissolve sticky deposits f this procedure fails to restore the normal performance replace the check valves with new ones 29 Maintenance Check Valves 30 Check valve replacement Turn off the pump Disconnect the inlet outlet tubing and detach the head from the pump Unscrew the inlet outlet check valve support screws which contain the inlet outlet check valve cartridges Take out the check valve cartridges if necessary by means of a clean compressed air flow Re insert the new check valve cartridges Then follow the above steps in reverse order Note that neither the check valve cartridges nor their support screws are inter changeable from inlet to outlet Tighten the support screws at the nominal torque of 7 N m which ensures tightness up to the maximum pressure In the absence of a dynamo metric wrench tighten up to the mechanical stop i e to the metal on metal contact Run the pump and check for leaks around the support screws Tighten any leaking support screw until the leakage stops Maintenance 6 Pisto
9. cation of replacement periods for maintenance parts according to the type of use intensive regular or occasional The data of this table assumes that the pump is working at half of its maximum flowrate and pressure The nature of the liquid and the pump head model have only a small influence on these figures If the pump head is installed on a 305 pump the time between each maintenance operation can be viewed by using the Info soft key in the pump menu see 305 User s Guide 27 Maintenance 28 Replacement periods for maintenance parts Use Intensive Regular Occasional Parts 168 h week 40h week 10 h week Piston seal 2 3 months 6 9 months 1 year 1300 2000h 1000 1400 h 500 h Set of check valves 3 6 months 1 year 2 years 2000 4000 h 2000 h 1000 h Piston assembly 6 12 months 2 3 years 5 years 4000 8000 h 2000 3000 h 2500 h Anti extrusion gasket 6 12 months 2 3 years 5 years 4000 8000 h 2000 3000 h 2500 h Return spring 1 year 2 3 years 5 years 8000 h 2000 3000 h 2500 h 6 Check Valves The Gilson check valves are supplied in clean pre mounted cartridges Each one is thoroughly checked and then packed separately S AJLA 42949 Reliable flowrates will be achieved only if the check valves are kept in good operating condition by proper care and maintenance The check valve cartridge must be cleaned or replaced if there is a check valve problem This can be detected by an a
10. he piston seal mounting tool which is composed of a seal pusher and a seal guide to install the new piston seal Set the guide onto the pump head with its bevelled edge out Insert the new piston seal in the guide with its spring side down Piston seal mounting tool Seal guide Seal pusher Piston seal Maintenance Press the seal pusher with your finger until the piston seal goes down to the pump head Re assemble the pump head by following the disassembling pump head instructions in reverse order Run in the new piston seal accord ing to Chapter 5 Referring to the pump head exploded view ensure the piston assembly and the spring spacer are correctly assembled i e spherical male into spherical female Line up the ball housing assembly the housing spacer and the head body with the matching hole or notch on the same side After screwing the ball housing assembly and the housing spacer onto the head body put the body on a flat surface to facilitate the mounting of the other parts 29S uoisid 33 Maintenance Piston Seal 34 Return Spring Due to fatigue of the steel the return spring see pump head exploded view should not be considered as having an unlimited lifetime We recommend that the spring is changed with every five piston seals used To change the return spring dismount the pump head by following the instructions Chapter 4 in reverse Unsc
11. hin the limits shown in the Figure overleaf pue 23 Precautions for Use Salinity and Acidity If you have to cross these limits occa sionally re passivate the stainless steel according to the following procedure Make up 100 ml of 50 nitric acid and pump 60 ml at 2 ml min e Stop the pump for 30 minutes Flush the system with water until acid has been removed pH higher than 6 pH Pd S 5 WSC Z zt 2L d 2 WTi _ a 0 01 01 1 10 Molarity of solid salt dissolved in water chloride ions for WSC WTi transition 24 Typical working range of each type of Gilson pump head according to salinity and acidity at ambient temperatures At low pH and high molarity it is necessary to use WTi only with halides ions But for example at pH 3 and 0 5 M NaCl the WSC is sufficient Precautions for Use Viscosity There is no direct relationship between the viscosity of the liquid to be deliv ered and the pumping capability of the head The two following points must be considered when dealing with high viscosity liquids Inlet pressure The pump head must be in charge to avoid cavitation and to be normally fed This may be performed with hydrostatic pressure by raising the reservoir or may require a slight gas pressurization of the reservoir Thixotropic test The check valve ball of the pump head must rapidly fall down in the c
12. n Seal The piston seal consists of a seal ring made of either graphite reinforced PTFE black or HDPE yellow and a spring made of Hastelloy C 276 wire or Elgiloy HT ribbon It must be changed whenever a piston seal leak age occurs This can be easily detected by the appearance of some liquid at the notch located at the bottom of the pump head 895 uoisid Guidelines to select the piston seal according to the liquid are given in Chapter 2 Disassembling the pump head Turn off the pump Disconnect the inlet outlet tubing Detach the pump head from the pump Referring to the exploded view page 13 dismount the pump head as follows Unscrew the threaded cover Take out the piston tail guide the piston assembly the spring spacer and the return spring Loosen the two screws on the back of the pump head and remove the ball housing and the housing spacer Remove the anti extrusion gasket 31 Maintenance Piston Seal 32 N bevelled edge out spring side in Carefully take out the piston seal If it remains tightly seated in the head body pry it out with care in order to avoid damaging the pump head body Once the piston seal has been removed it must not be re used Installing a new piston seal nspect the piston and the area around the piston seal to ensure that they are clean undamaged and completely free of any particles Referring to the Figure below use t
13. ng spacer Return spring Spring spacer Threaded cover Ball housing assembly Inlet check valve cartridge Piston assembly Inlet check valve support screw Piston tail guide Inlet oe 50SC 100SC 25SC 13 Description 14 Setup 4 N Switch the power off before mounting or remov ing the pump head on or from the pump Carefully insert the pump head into the opening of the pump Note that at the bottom of the head body there is a notch This must be fitted onto the matching notch pin just below the opening for the pump head Holding the pump head in place with one hand set the clamp diagonally over the head Turn the clamp clockwise into position in the slots on either side of the pump head Finger tighten the thumb screw until the clamp holds the head securely Make sure that the clamp ends are secured in their slots on both sides 15 Setup 4 Tubing Connections 5 di Are 25SC S S tubing i d 1 mm 508C PTFE tubing i d 2 mm 1005C gt E cBsi Xsata E SSI qp SSI screw r 5SC 37 m o 10SC S S tubing i d 0 5 mm ina 16 Inlet tubing assembly 3645275 36075002 NN Filter 10 Inlet tubing assembly 3645357 Filter 20 um 36410649 j Inlet tubing assembly 3650069 55 105 255 505 100SC Precautions for use 5 This chapter describes the precautions to take in order to ensure o
14. npacking and Standard Accessories 1 Minimum Required Configuration Standard accessories Model 55 10SC 2556 505 100SC Qty Reference 360510 360520 360522 360524 360530 Inlet tubing assembly 3645275 3645275 3645357 3650069 3650069 1 FEP additional inlet tubing none 49948392 499493922 PTFE outlet tubing none 36425019 1 350 x 3x 2 mm SSI screw 49031401 2 SSI ferrule 49031402 2 10 ml syringe 36460058 none 1 PVDF coupling 1 4 28TPI none F1410153 1 Washer 3645329 none none 1 Piston seal mounting tool 36620122 36620123 36620290 36620291 36620292 1 S S outlet tubing 250 mm 49932259 49934259 1 Allen wrench 3 mm 4320302 1 PVDF coupling 1 4 28TPI none F1410153 F1410156 1 User s Guide LT801077 1 3645275 PTFE inlet tubing 1000 x 2 x 3 mm equipped with a 10 um stainless steel filter a 3 way prime valve and FEP inlet tubing 60 x 1 6 x 3 mm 3645357 PTFE inlet tubing 640 x 2 x 3 mm equipped with a 20 um stainless steel filter 3650069 FEP inlet tubing 1000 x 3 x 4 mm equipped with a 20 um stainless steel filter 49948392 FEP additional inlet tubing 1000 x 2 x 3 mm 499493922 FEP additional inlet tubing 1000 x 3 x 4 mm 499692002 FEP additional inlet tubing 2000 x 4 x 5 mm not supplied available as an additional accessory only for 100 SC Additional Accessories 2 This section lists all the additional accessories that can be used with the pump heads
15. onsid ered liquid at a speed of about 10 cm s otherwise the pump head will not work satisfactorily Such a test can be performed in a graduated cylinder Note that a non abrasive suspension of 40 centipoises can be successfully pumped with the Model 10SC head For high viscosity and or high density liquids a spring loaded check valve kit 3650201 is available as an additional accessory for Models 5SC 10SC 255 and 10WSC N AjJSOOSIA 25 Precautions for Use 5 Temperature 26 Temperature The maximum temperature of the liquid pumped through the head is limited by the piston seal and by the front panel of the module Gilson pump heads can operate from 20 up to 100 C provided that the liquid pumped is chemically compat ible with the liquid contact parts see Chapter 7 Technical Data A complete thermostatic kit is available for heads 5 10 and 25 see Chapter 2 Additional Accessories For Liquids at Non ambient temperatures Maintenance 6 General maintenance of a Gilson piston pump head irre spective of type and model consists of cleaning check valves and filter replacing parts subject to wear and tear piston seal check valves piston assembly anti extrusion gasket and return spring The case of unattended continuous operation of automatic systems has an increasing practical importance For this reason Table Replacement periods for maintenance periods gives an indi
16. ptimal use of your pump head 17 Precautions for Use 5 Solvent Filtration 18 Solvent Filtration The inlet filter attached to the end of the PTFE inlet line must be used with all solvents to protect the check valves In addition it is recommended to include one unit of the high pressure in line filter 4970050105 offered as an additional accessory in any HPLC system This efficient filter is to be placed between the prime purge valve and the injection valve It provides a durable protection to the injection valve and to the column Precautions for Use Use of a New Pump Head or a New Piston Seal Whenever using a new pump head or a new piston seal it is strongly recom mended to follow the running in procedure given below which can be conveniently implemented using the prime purge valve Run the pump unloaded for 1 2 minutes at 2096 of nominal flowrate with methanol Run the pump at maximum operat ing pressure for 4 to 5 minutes checking for any leaks Repeat the first step but for 30 minutes 895 uojsid MON peaH M N esf 19 Precautions for Use 5 Solvent Degassing 20 Solvent Degassing Solvents should be degassed to avoid air bubble formation in the pump head or in the detector cell Vacuum filtration or helium sparging are much more efficient than ultra sonic degassing Solvent degassing also contributes to better baseline stability with the UV
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18. rew the threaded cover and remove the piston tail guide the piston assembly the spring spacer and the spring Replace the spring and reassemble the pump head Maintenance Maintenance Kit For your convenience a maintenance kit for each pump head is available Part Number Description 364005 Maintenance kit for 5SC pump head 364010 Maintenance kit for 10SC pump head 364012 Maintenance kit for 25SC pump head 364013 Maintenance kit for 50SC pump head 364015 Maintenance kit for 100SC pump head 35 Wy Maintenance 36 Technical Data T Pump head Flowrate range Pressure range MPa Stroke volume model ml min 302 303 305 ul 5SC 0 010 5 0 1 42 0 1 60 39 4 10SC 0 050 10 0 1 21 0 1 60 78 8 25SC 0 125 25 0 1 84 0 1 28 197 50SC 0 250 50 0 5 42 0 5 14 394 10056 0 500 100 05 21 0 5 7 788 Minimum indicated to obtain specified precision and accuracy Flowrate adjustable down to 0 01 of maximum flowrate 1 MPa 10 bar 145 psi 37 Technical Data Liquid Flow Data 38 Liquid Flow Data Liquid Contact Materials 316L stainless steel titanium sapphire or ceramic ruby PCTFE polychlorotri fluoroethylene PEEK PTFE Hastelloy C276 or Elgiloy HT Flowrate Precision At 20 and over full working range coefficient of variation is 0 1 to 0 6 with aqueous solutions or hydro organic polar solvent mixtures and 0 3 to 1 with
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