Thermo Scientific Matrix Hybrid Pipette User Manual
Contents
1. 1604 8604 8609 NYNA Ie9n5 l 3 pez ON MET Z604 8906 1604 8604 8609 GZ909N3 yunuuu 2661 L 28008N3 Ayiquedwoo SUOISSIUJ3 oneuBeulo4129 3 L LLOSSN3 ZZOSSNA 933 9 68 ayedid edoun3 puq H 2601 8906 6601 suojeinBeu ejes eDejoA o1 gt gt 9601 9609 6609 661 L 0L0L9N3 Ouuree e w JOulJoJSUeJ p1epue s 9Anoailq yew eAouddy yonpold peuonueui sysew pue s eAo4dde ay JO sjonpoJd e911109 9 104 s s 1 jojes paunba ay passed aney mojaq peisi sjonpoJd eu sjonpoJg Jno jo Bus jajes 24 25377 Rev 2 0 Hybrid Pipetting System Compatibility Chart Tips packed 10 racks of 96 Hybrid Pipettes Tip Volume Range Single 8 Channel Channel e 12 5yl 0 5 12 5ul 1081 2310 e 30pl 2 30pl 1082 2312 125yl 10 125ul 1083 e 300yl 20 300pl 1084 2315 e 1250pl 100 1250pl 1085 Bulk 500 tips Bulk 1000 tips 8 Racks of 96 tips order online It s easier than ever to order Thermo Scientific Matrix products online with a credit card or purchase order Choose from our complete line of pipettes pipette tips microplates and tubes at www thermo com matrix 2007 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Part of Thermo Fisher Scientific Compatible ClipTips 12 Channel Bulk Non sterile Sterile Filter Sterile Racked Racked Racked 2320 71204 7121 712. See chart below for voice input examples for each volume range 100 12501 TALK Thermo Note In Speaker Dependent Mode the pipette registers voice input differently The pipette will prompt each digit with a single beep tone Voice the desired digit after Ex Press and Hold Talk button lt beep gt 1 lt beep gt 2 lt beep gt 3 lt beep gt 4 Once the plunger has completed volume adjustment the selected volume will appear on the display and a single beep will indicate that it is ready to begin pipetting OU 100 12501 Thermo button once to reset the error message The 100 1250 Thermo selection Talk button to re Programming the Hybrid Pipette Voice Recognition Note When the pipette indicates an ER 3 message press either Scroll button to reset the error message in order to continue see section G 2 Fault Recovery for more details Without resetting the error attempting to Press and Hold the Talk button may result in continued errors Incorrect E 4 y y I 1 A A Correct distance of pipette when using Incorrect distance of pipette when using Voice Recognition Voice Recognition Pipette Desired Voice Displa Volume Volume Input p 0 5ul 0 0 5 0 00 50 0 5 12 5ul 12 5ul 1 2 5 0 12 50 2 0ul 0 2 0 0 02 00 2 30ul 30ul 3 0 0 0 3
3. buttons user defined volume pre sets or with state of the art voice recognition VR technology Enhanced accuracy and precision is obtained with an intelligent processor controlled volume adjustment that accurately directs each volume range with a precise control algorithm This technology allows for precise pipetting throughout the entire range of the pipette Calibration is simple Use One Point calibration when pipetting a specific volume consistently Use Two Point calibration when pipetting throughout the entire volume range of the pipette The pipette can easily be restored back to its original factory calibration points at any time Preset volumes with the QUIK set user defined volume adjustment feature This saves time when toggling between two or more volumes with the same pipette Ergonomic light plunger force allows superior control when pipetting Voice recognition commands and user defined preset volumes save time by eliminating strenuous knob twisting involved with other manual pipettes Electronic adjustment of the volume guarantees consistent precise pipetting Exclusive ClipTip interface delivers assurance of an effective seal without the need to apply excessive force or having to bang on the pipette tip Simply touch the pipette to the ClipTip and it locks in place it will only release when ejected regardless of application pressure ClipTips are binary so they are either on or off the pipette Hybrid
4. functioning low batt Device Low Battery ee Critical Message Indicator Alternates with m Device normal display to indicate importance See Critical Msg Ind next item as an example alt with Sio Depleted battery low batt Critical p 18 Thermo Trouble Shooting and Maintenance G 4 Recharging the Batteries The Hybrid pipette is equipped with a factory installed rechargeable long lasting Lithium Ion battery It allows for long life between charges with no battery memory effect reduced battery capacity associated with other battery types The charging contacts on the pipette can be used with the provided Universal Power Supply w Charge Clip Part No 1092 or with the Revolving Carousel Charge Stand Part No 1091 Charge the pipette for 2 hours for a complete charge 80 charge after 1 hour Simply connect the charging contacts of the pipette to the Power Supply or Carousel A flashing battery light will appear on the LCD screen to indicate the battery is charging Appendices WARNING Ensure that the charge clip or carousel power transformer is connected to a power receptacle that provides voltage and current specified by Thermo Scientific Use of an incompatible power receptacle can cause shock and fire hazard H Appendices H 1 Calibration Suggestions Y Calibrate the pipette for the liquid Hybrid pipettes are factory calibrated to deliver accurate volumes with distilled water at 21 C a
5. 0 00 10ul 0 1 0 0 010 0 10125 V Eccl 125ul 1 2 5 0 125 0 20yl 0 2 0 0 020 0 20 300yl 300ul 3 0 0 0 300 0 100ul 0 1 0 0 0100 100 1250ul 1250ul 1 2 5 0 1250 Thermo M Programming Voice Recognition E Voice Recognition Menu The Voice Recognition Menu is used to select the voice recognition language mode The pipette has two voice modes Speaker Independent and Speaker Dependent Speaker Independent Mode is for English speaking users using American British or similar accents Speaker Dependent Mode allows the user to input any language accent or sound as a numeric value within the pipette s memory Up to three users can store their individual voice patterns in the language memory locations USR1 USR2 and USR3 These options will appear in the VOIC menu once trained using the corresponding Learn options LRN1 LRN2 or LRN3 i e LRN1 trains USR1 etc The user can access the language training by choosing the Learn options from the VOIC menu See section E 1 below for more details E 1 Navigating the Voice Menu Options When entering the Voice Menu the first option ENGL will be displayed Use the Scroll buttons to navigate through the Voice Menu and tap either Talk button to enter an option Press either Scroll button Access the Main Menu by Select the voic menu by pressing either Talk pressing the NN to navigate 100 1250 vol
6. 22 7165 2322 71204 7121 7122 AS 7130 731 7132 7135 2325 7130 7131 7132 7135 7140 7141 7142 7145 Pipette Service and Repair Expert Calibrations Preventive Maintenance and Repair Services Our factory trained certified technicians perform electronic and manual pipette services Simply ship one or multiple pipettes in a pre paid pipette service box to our facility Most calibrations are performed in less than 48 hours Our expertise extends to all pipettes in the industry from any brand model or manufacturer In North America contact us at matrix technicalservice thermofisher com or 888 363 6631 to get your free shipping packages North America Tel 800 345 0206 email matrix info thermofisher com Europe Tel 44 0 161 486 2110 email matrix eu info thermofisher com Asia email matrix ap info thermofisher com web www thermo com matrix SCIENTIFIC
7. ES Wipe NO NO NO WARNING Do not expose batteries to temperatures above 60 C 22 Thermo Technical Support Equipment Decontamination Verification Form Serial RP PO Prior to service of equipment that may be contaminated with biohazardous materials potentially biohazardous materials or radioactivity the user must first decontaminate it This decontamination procedure will include the following as appropriate please check one This equipment has not been used in a laboratory location exposing it to biohazards or radioactive materials This equipment was APPROPRIATELY DECONTAMINATED FROM BIOHAZARDOUS MATERIALS WITH Example Autoclave 10 Bleach Ethylene Oxide Instruments must be decontaminated both externally and internally LI This equipment was APPROPRIATELY DECONTAMINATED amp TESTED FOR RADIOACTIVITY BY Example wipe test with results 3H 14C a emitters Geiger counts etc NAME print PHONE SIGNATURE DATE Federal regulations require that all instruments sent for service shall be free of radioactivity and biohazards The Technical Service group reserves the right to refuse to work on this equipment if these procedures are not performed Customer Return Shipping Address Return fax 603 577 7682 FRM029 Rev D Thermo 23 Technical Support c60L 8906 nsnpu pue apes uj0u023 JO MSIUIA asoueder meJ Ajajes jeueje N pue souelddy
8. Pipette and ClipTips Overview B Hybrid Pipette and ClipTips Overview The pipette has three modes of operation for volume adjustment Volume Scrolling Mode The volume can be manually adjusted using the Up and Down Scroll buttons Pressing and holding will increase the speed at which digits change on the display Volume Scrolling Mode is always on QUIK set Mode The user may toggle between six preset volumes that are stored in memory These presets are easily deleted and replaced by the user as needed QUIK set Mode is the factory default mode and when disabled Voice Recognition Mode is selected Voice Recognition Mode Pressing the TALK button allows the user to select the desired volume by speaking aloud to the pipette Voice Recognition Mode has two variants either Speaker Independent Mode which recognizes standard English input or Speaker Dependent Mode which requires the user to train the pipette to a desired language or regional accent Voice Recognition Mode is disabled when QUIK set Mode is selected See Programming and Operation Section D ClipTips ClipTip racks featuring multi colored labels are compatible with several volume ranges of pipettes Use the chart below to match the color of the pipette with the corresponding ClipTip rack label Pipette Color Red Purple Yellow Blue Green Tip Volume 12 5yl 304 125yl 300pl 1250ul Range 0 5 12 5ul 2 30ul 10 125ul 20 300ul 100 1250ul Lt G
9. Recognition ModE sassisk anae STRIS EEE 9 Set the Volume Using Voice Recognition 10 E Voice Recognition Menu u 12 Navigating the Voice Menu Options u u L UR a au ahua aasawa 12 Speaker Dependent Training sequence 14 F Calibration 15 One Point Calibrations enirn cb ret Dee ee ba reete mx ob ce b Peppe os 15 TWosPoimt Galibraliori EE 16 G Troubleshooting amp Maintenance eene 17 Pipette Maintenance and Cleaning 2 ccccscceceeseeeseeeeeeseneeeeeeaeeeseaeeeseaaeesseeaeeessaeees 17 Fault RECOVGRY P HM 18 TMOUDIESHOOUNG usu kin a 18 Recharging the Battenes u aid einstein 19 i Thermo H Appendices J enne nnne nnn nenne nennen nera Calibration SUYYESTIONS oooonncocininccccnocccanoncccnnoncccnnancconnonncnnnnnn ca nnnnc cana NE a RD aaia Eae 20 Pipetting SUGGESTIONS coria e ERI REEKUISED ERR E LUN SE SEL eee 20 Technical SUDOR randstad coto 21 VAULT DEO DLE 21 Thermo Congratulations You have purchased the most technologically advanced handheld pipetting System on the market today The Thermo Scientific Matrix hybrid pipette combines the benefits of an electronic pipette with the control and lightweight feel o
10. Thermo Scientific Matrix Hybrid Pipette User Manual Trademarks and Copyright 2007 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries No part of this publication may be reproduced stored in a retrieval system or transmitted in any form or by any means in whole or in part without the prior written permission of Thermo Fisher Scientific Inc Third party products are registered trademarks of their respective companies in the United States and or other countries Thermo SCIENTIFIC CONTENTS Congratt latloris iieri A cra cet Leere riui spen iv Before You Begin cuina lali e aatan iaeiiai iv A Introduction rente iii 1 B Hybrid Pipette and ClipTips Overview 2 hijo A 3 Iiybticl PIDBllG toser M UE 3 Hazards and PrecaltiNS iia bre e Pee aco ep E rede etr ERR End 4 C Applying TIPS common iii aida 4 How to Apply Clip TIDSS cocinada 4 Ordering Guide enciende a t dee t ee ad 5 D Programming and Operation u u nnn 5 Set the Volume Using the Scroll Buttons esssssesssseeseeeeeneeeeennenne 5 Navigating the Main Menu Options cooooccconoccccnoccccnnoncnconononcnnanononno conan ono nnnrncnnnnn nc nannnncnns 6 Programming the QUIK set Volume List cerent nnno rn hne thin tana 6 Turn On Voice
11. cremental volume scrolling The Voice Recognition Mode is turned OFF while this menu option is ON Up to six 6 QUIK set volumes may be stored in memory Follow the diagram below for programming instructions D 3a Turn On Off the QUIK set Mode Note QUIK set is the factory default setting Navigate to QUIK in the Main Menu and select Access the Main Menu by pressing the 100 1250 QUIK Scroll Up or Down to toggle QUIK set mode ON or OFF Scroll buttons simultaneously then release the option by pressing Scroll Button Scroll Button Programming the Hybrid Pipette 100 12501 Tap the Talk button The selection is stored once to select this in memory and the Thermo mode pipette returns to f ON or OFF normal operation D 3b Add a Volume in the QUIK set List QUIK set Mode Press and Hold JE Select the desired volume MACC E on the display AS displayed Button Button If the list is not full the current volume selection is added to the QUIK set list and a beep signals the i selection has been added to The pipette will memory return to normal operation or Button If this list is full the current volume is rejected and Continue holding the a FULL message is Talk button until displayed O00 0 is displayed and a beep signals the volume has been added Note Before a new volume can be added to a FULL list a previously set volume must be deleted s
12. ct technical support 888 363 6631 for instructions Noth America 888 363 6631 Europe 44 0 161 486 2112 Asia asia matrixtechcorp com Thermo zi Technical Support Recommended Equipment Decontamination Methods The following table lists the proper decontamination procedures for returning equipment to Thermo Fisher Scientific for service Heat at Autoclave 65 C 70 30 10 Ethylene 121 C Product Component 10 Hr IPA Water Bleach Oxide 15 min Multichannel Matrix Electronic Pipettes Housing Exterior YES Wipe Wipe YES NO Piston Cylinder Assembly YES YES NO YES YES Tip Manifold YES YES NO YES YES Battery NO Wipe NO NO NO Power Supply NO Wipe NO NO NO Single Channel Matrix Electronic Pipettes Pipette w Battery Removed Yes Wipe Wipe YES NO Lower cylinder YES YES YES YES YES Piston do not remove YES YES NO YES NO Power Supply NO Wipe NO NO NO Matrix Hybrid Pipettes Housing Exterior YES Wipe Wipe YES NO Power Supply NO Wipe NO NO NO Matrix Memowell Power Supply NO Wipe NO NO NO Housing Exterior YES Wipe NO YES NO Matrix Manual Pipettes Lower assembly YES Wipe NO YES YES Housing Exterior YES Wipe Wipe YES NO Matrix Serological Pipettes Nosecone YES YES YES YES YES Silicone Rubber Holder YES YES Wipe YES YES Filter Replace Replace Replace Replace Replace Battery NO Wipe NO NO NO Power Supply NO Wipe NO NO NO Housing Exterior Y
13. d in order to be autoclaved or replaced Tip Fitting I 2300 ClipTip Fittings 1250 ul Pack of 24 2301 ClipTip Fittings 125 300 ul Pack of 24 2302 ClipTIp Fittings 30 ul Pack of 24 2303 ClipTip Fittings 12 5 ul Pack of 24 17 Thermo Trouble Shooting and Maintenance G 2 Fault Recovery a If a non critical error condition is being reported clear the error by tapping any Scroll buttons b If a critical error condition is being reported clear the condition by pressing the left and right Talk button simultaneously for five seconds This will trigger the pipette to clear the error and perform an initialization sequence If no further errors are detected the pipette will return to its previous state before any errors occurred G 3 Troubleshooting Error code list Error Code Type Indication Time Out sequence aborted because of ER 1 User E a User inactivity Calibration entry invalid the value exceeds ER 2 User the defined min max limit for the product version Volume entry invalid the value exceeds the defined min max limit for the product ER 3 User i M E I version This will only happen while in voice recognition mode ER 4 Device Mechanism jam condition ER 5 Device Mechanism positioning error ER 6 Undefined ER 7 Undefined Device ER 8 ee Electronics Fault Critical ER 9 Device Voice Recognition mode is unavailable or Critical the circuitry is not
14. e is now deleted and a new volume may be stored Follow the instructions for adding a QUIK set volume section D3b D 4 Turn on Voice Recognition Mode Voice Recognition VR and QUIK set modes can not be in operation at the same time Toggle between the two modes by accessing the main menu and turning off QUIK set mode to enable the Voice Recognition Enable Voice Recognition by using the following steps 1 Press both Scroll buttons then release to access the main menu 2 Press either Scroll button to navigate to the QUIK option 3 Press the Talk button to select the option 4 Press either Scroll button to toggle to Off 5 Press the Talk button to disable QUIK set mode and enable VR Programming the Hybrid Pipette D 5 Set Volume Using Voice Recognition Press and Hold either Talk button While TALK appears on the display speak the desired volume while continuing to hold the Talk button Release the Talk button after speaking all digits each beep Busy will display while the plunger moves to the selected volume SQ 100 12501 BUSY Thermo WF 100 12501 If the pipette cannot process the information an ER 3 message Thermo will flash Ly Scroll Button 10 Tap either Scroll pipette will return to the previous volume Press and Hold the enter a new volume Turn Voice Recognition on using the Main Menu options see section D 4
15. ee section D3d Programming the Hybrid Pipette D 3c Select a Volume from an Established QUIK set List Access the QUIK set volumes by pressing the The first volume in the QUIK set list will appear on 100 1250 QUIK QUIK set Mode Navigate through the QUIK set list using the Scroll buttons Talk button until Qg uic is displayed then release the display Scroll Button Button k Button Tap either Talk button to select the desired QUIK set volume Once the plunger has completed volume adjustment the selected volume will appear on the display and a single beep will indicate that it is ready to begin pipetting 100 125041 BUSY Therma BUSY will display while the plunger moves to the selected volume Note Before a new volume can be added to a FULL list a previously set volume must be deleted see section D3d Programming the Hybrid Pipette D 3d Delete a Volume from the QUIK set List Access the QUIK set list by pressing the Talk button until QUIK is displayed then release Press either Scroll button to navigate to the volume to be deleted QUIK set Mode 100 125041 QUIK The first volume in the QUIK set list will appear on the display 100 1250 Press and Hold either Talk button Thermo Button 0000 DO D P will appear on the display to indicate the deletion Talk Button The previously stored volum
16. f a manual The hybrid pipette works exclusively with the Matrix ClipTips which provide a unique binary tip interface that locks or clips the tip onto the pipette for stable straight mounting every time Simply touch the hybrid pipette to the Matrix ClipTip and it locks in place it will only release when ejected regardless of application pressure The Matrix hybrid features three methods of quick volume adjustment including simple incremental scroll buttons user defined volume pre sets or with voice commands using state of the art voice recognition VR technology Before You Begin Before using the hybrid pipette verify the contents of the package This package should contain the following items Hybrid Pipette Operator s Manual Universal Power Supply with Charge Clip Quick Start Guide Warranty Card Accuracy Precision Data Sheet Sample ClipTips If items are missing contact a Thermo Scientific representative The hybrid pipette will need to be charged for at least one hour prior to first use Introduction A Introduction The Thermo Scientific Matrix hybrid pipette combines the benefits of an electronic pipette with the control and lightweight feel of a manual The hybrid pipette works exclusively with the Matrix ClipTips which provide a unique binary tip interface that locks or clips the tip onto the pipette The Matrix hybrid features three methods of quick volume adjustment including simple incremental scroll
17. ic Scroll buttons through the simultaneously menu options then release Scroll Scroll Button 12 Thermo Programming Voice Recognition Option Description Default voice recognition setting for any English speaking user ENGL Speaker Independent Choose this option once the pipette is trained in Speaker Dependent Mode using LRN1 USRI this selection is not available in this menu until the LRN1 training has been completed Choose this option once the LRN2 feature has been trained USR2 this selection is not available in this menu until the LRN2 training has been completed Choose this option once the LRN3 feature has been trained USR3 this selection is not available in this menu until the LRN3 training has been completed Enter this option to train the pipette for the USR1 location ERNI Speaker Dependent Enter this option to train the pipette for the USR2 location LRNZ Speaker Dependent Enter this option to train the pipette for the USR3 location PENS Speaker Dependent NONE Disable the voice recognition mode EXIT Exit this menu Thermo 15 Programming Voice Recognition E 2 Speaker Dependent Training Sequence In order to input a new language in Speaker Dependent Mode select the LRN1 LRN2 or LRN3 option from the VOIC Menu options see sections E and E 1 above The pipette will begin a training sequence prompting the user to input new command
18. lored labels are compatible with several volume ranges of pipettes Programming the Hybrid Pipette C 2 Ordering Guide ClipTips and Hybrid Pipetting System Tips packed 10 racks of 96 Tip Range Single 8 12 Bulk Non sterile Sterile Filter Sterile Volume Channel Channel Channel ClipTips ClipTips ClipTips ClipTips 1250ul 100 1250u 1085 7140 714144 7142 7145 300ul 20 3000 1084 2315 2325 7130 7131 7132 7135 125ul 10 125u 1083 7130 7131 7132 7135 30yl 2 30ul 1082 2312 2322 7120 7121 7122 7175 12 5ul 0 5 12 5ul 1081 2310 2320 7120 7121 7122 7165 Bulk 500 tips a Bulk 1000 tips 8 Racks of 96 tips D Programming and Operation The pipettes volume can be set either by using the Scroll buttons Voice Recognition or by user defined QUIK set volumes The Voice Recognition Mode comes ready to use for the English language If English is not the language of choice the Hybrid pipette can support all languages with user training When toggling between one or more volumes consistently use the QUIK set volume mode see section D3 for programming QUIK set D 1 Set the Volume Using the Scroll Buttons Note The speed of the volume Scrolling will increase as the scroll buttons are continuously held and will stop when the button is released Once the plunger has completed Press either BUSY will scroll button to display while the change volume plunger moves volume adjustment increment Up to the
19. nd 5096 humidity Other liquids have there own unique specific gravity and vapor pressure By using the calibration procedures described in section F you can pipette different liquids with a higher degree of accuracy and reproducibility H 2 Pipetting Suggestions 4 Pipette at a constant temperature Allow liquids and equipment to equilibrate to ambient temperature Volume aspiration with air displacement pipettes varies with air pressure relative humidity and vapor pressure of the liquid all of which are temperature dependent Y Attach tips with slow even pressure When applying ClipTips avoid banging or applying excessive force to the tip s ClipTips have been specifically designed to match the tip fittings on Hybrid pipettes Y Pre wet the pipette tip Aspirate and dispense two to 5 times before aspirating the liquid to increase humidity within the tip this will reduce sample evaporation that may result while pipetting lower volumes This technique is useful when pipetting liquids that have low vapor pressure and or high surface tension Y Immerse tips fully This will avoid aspiration of unwanted air and the clinging of excess droplets to the outside of the tip Tips should be immersed approximately 1 4 inch 0 6 cm below the meniscus and away from the container surfaces V Use consistent plunger speed The Hybrid pipette like all precision instruments will generate more reproducible results when operated with attention
20. o Charging n Contacts U Color represents volume range Voice Recognition Microphone e Right Talk Button lt Scroll Up Button Color represents i ume range Keypad N Plunger aaa l Color represents 4 volume range Ergonomic Hand Rest Tip Ejector ClipTip fitting Applying Tips B 3 Hazards and Precautions WARNING Ensure that the Charge Clip or Carousel power A transformer is connected to a power receptacle that provides voltage and current specified Thermo Scientific Use of an incompatible power receptacle can cause shock and fire hazard WARNING Do not autoclave the unit Extreme heat will damage electronic components CAUTION Do not leave the pipette exposed to U V light for extended periods Extended exposure will cause discoloration of the plastic housing CAUTION Do not immerse the unit in liquid Fluid seepage can damage internal components CAUTION Do not clean the keypad with bleach solution or other solvents Caustic cleaning solutions can damage the keypad C Applying Tips C 1 How to Apply ClipTips Simply touch the pipette to the ClipTip When the tip clips onto the pipette it is ready for use Use the ejector button to eject the ClipTip On multi channel units gently roll the pipette forward and back to ensure that the tip fittings contact all the tips in the row ClipTips racks featuring multi co
21. or to entering the Main Menu options Two Point calibration is used when the pipette will be used for more than a single volume Two Point calibration CAL2 is the factory default setting for all pipette models dH20 at 21 C and 50 humidity CALF mode resets the pipette to factory default calibration F 1 One Point Calibration 1 Use the Scroll buttons to select the desired volume for One Point calibration 2 In the Main Menu options see section D 2 navigate with the Scroll buttons and select CAL 1 by tapping either Talk button once The pipette will issue a single beep and begin alternating the display between the CAL 1 status message and the calibration volume This will continue until the calibration results are entered below in Step 3 Note To terminate the calibration sequence tap either Talk button do not adjust displayed value with the Scroll buttons or calibration will be altered The pipette will return to standard operation without making a change in calibration 3 Collect 10 samples weigh each result and determine the average a If necessary adjust the calibration value by using either Scroll button until the desired value appears i e your calculated average Tap the Talk button once the pipette will process the value b If there is no adjustment necessary tap the Talk button once The pipette will return to standard operation without making a change in calibration When the calibration resul
22. reen Red Purple Yellow Blue Thermo Thermo SCIENTIFIC SCIENTIFIC 2 5 12 5 30pul 125 300u1 Non Sterile ClipTips Non Sterile ClipTips Cat 7121 Lot 5554441 3 Cat 7131 Lot 5554441 3 MATRIX MATRIX Thermo 1250ul1 Non Sterile ClipTips Cat t 7141 Lot 5554441 3 MATRIX Green B 1 Keypad Hybrid Pipette and ClipTips Overview Talk Button Use the left or right Talk buttons to deliver voice commands Scroll Buttons Use the Up or Down Scroll buttons to increase or decrease desired pipette volume LCD Screen The display shows the current volume setting and pipettes status information such as low battery and user interface information VR Microphone Speak into the voice recognition microphone when utilizing voice commands while holding down the Talk button The pipette should be held at an optimal distance of 12 18 inches away from the mouth LCD Left Talk Button Scroll Down y Button B 2 Hybrid Pipette Color Represents volume range for that handle Reference the color chart on the previous page for volumes Plunger Manual aspiration and dispensing of liquids Keypad Interface for programming the hybrid pipette Tip Ejector Ejects the ClipTips from the pipette when pressed Ergonomic hand rest Provides a comfortable grip while pipetting Charging contacts Contact point for charge clip or charge carousel for revitalizing the battery when low or depleted FaN 100 1250 Therm
23. s for each digit from O through 9 The pipette is capable of storing up to 3 different user profiles that will best respond to that individual other users may experience non optimal performance while these speaker dependent setting are in use Voice Mode Navigate through the voice menu using the Scroll buttons to locate the LRN 1 LRN1 or LRN2 or LRN3 option and select it by tapping the Talk button once 0 is the first digit displayed for training Press and Hold The pipette will prompt your voice the Talk button 4100 12501 input with a beep Speak the until you see indicated digit in the language of TALK on the Thermo choice The pipette will prompt you 2 display then PN 3 times to make sure it understands release CAY your voice input correctly Button The screen will display The next Em D 0 0 T to M m indicate the digit in the pipette is processing the information Sequence is Thermo I A displayed Continue this process until all digits have been trained from 0 9 14 Thermo Calibration F Calibration The Hybrid pipette has two calibration modes CAL 1 for one point calibration and CAL2 for two point calibration One Point calibration is used when pipetting a specific volume consistently When CAL1 is selected the pipette will assume that the current volume is the desired One Point calibration volume Make sure you have selected your volume pri
24. selected the selected volume to increase Rx volume 100 1250 1 will appear on the display and a single beep will indicate that it is ready to begin pipetting and Down to BUSY decrease Programming the Hybrid Pipette D 2 Navigating the Main Menu Options Access the Main Menu by pressing and holding the Scroll buttons simultaneously then release MENU will immediately appear on the screen Use the Scroll buttons to navigate through the menu options and the Talk buttons to enter or select options Option Description QUIK Enter the QUIK set mode to enable or disable this function CALI Enter this option when using the One Point calibration caLz Enter this option when using the Two Point calibration CALF Tap Talk to re establish the factory calibration voic Enter the voice recognition menu for user training disabling this mode or changing previously programmed users INFO Displays current software version and settings EXIT Exit this menu If there is no button activity for 10 seconds the pipette will return to its previously set functions While in either CAL1 or CAL2 mode there are thirty minutes to complete the calibration process Refer to section F for calibration instructions and to section E for the VOIC menu navigation and options D 3 Programing the QUIK set Volume s This mode is used to pre program volume settings in memory reducing the need for in
25. to detail and proper technique Depress and release the plunger smoothly and with consistent speed when dispensing each sample 20 Thermo Y Pause consistently Pause with the tip in the liquid for about one second after aspirating the sample to allow the liquid enough time to finish moving into the pipette tip after the plunger stops Otherwise the volume will be lower than it should be V Tip touching During the dispensing of a liquid touch the tip against the side of the receiving container to prevent any liquid from remaining on the tip Technical Support Please contact technical support direct in North America at 888 363 6631 or matrix technicalservice thermofisher com and in Europe at 44 0 161 486 2112 or matrix eu support thermofisher com Warranty The Hybrid pipette is warranted to the original purchaser by Thermo Fisher Scientific Inc to be free of defects in materials and workmanship for a period of twelve 12 months from the date of purchase providing that it has been operated according to the instructions not abused or misused that the serial number has not been removed and that the instrument has not been disassembled except the tip fitting for autoclaving No other warranty is expressed or implied Upon receipt of your instrument please complete and return the warranty card No pipette should be returned without a prior Return Good Authorization from Thermo Scientific Should a unit need to be returned conta
26. to the second calibration position while displaying a BUSY message e An alternating PT 1 calibration value is displayed It will remain until all calibration results are entered below in Step 3 If the results are not entered within 30 minutes the pipette will return to the previous calibration settings 3 Collect 10 samples weigh each result and determine the average a If necessary adjust the calibration value by using either Scroll button until the desired value appears i e your calculated average The PT 2 status message disappears when you begin to scroll to the calibration value Tap the Talk button once the pipette will process the new calibration value 16 Thermo Trouble Shooting and Maintenance b If there is no adjustment necessary then tap the Talk button once The pipette will return to standard operation without making a change in calibration When the calibration results are stored a beep will sound and the pipette will display DONE to complete the sequence G Troubleshooting and Maintenance G 1 Pipette Maintenance and Cleaning The exterior can be wiped clean periodically with a soft cloth moistened with methyl alcohol with bleach solution or other solvents Extreme heat can damage the display and other components Caustic cleaning solutions can damage the keypad d CAUTION DO NOT AUTOCLAVE entire unit or clean the keypad Note The tip fitting s can be easily unscrewe
27. ts are stored a beep will sound and the pipette will display DONE to complete the sequence 15 Calibration F 2 Two Point Calibration 1 In the Main Menu options see section D 2 navigate with the Scroll buttons and select CAL 2 by tapping either Talk button once The pipette will drive the piston assembly to the first calibration position while displaying a BUSY message An alternating PT 1 and calibration value is displayed It will continue to alternate until all calibration results are entered below in Step 2 If the results are not entered within 30 minutes the pipette will return to the previous calibration settings Note To terminate the calibration sequence tap either Talk button do not adjust displayed value with the Scroll buttons or calibration will be altered The pipette will return to the previous calibration settings 2 Collect 10 samples weigh each result and determine the average a If necessary adjust the calibration value by using either Scroll button until the desired value appears i e your calculated average The PT 1 status message disappears as you begin to scroll to the calibration value Tap the Talk button once the pipette will process the new calibration value b If there is no adjustment necessary then tap the Talk button once The pipette will return to standard operation without making a change in calibration The pipette will drive the piston assembly
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