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1. 7 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 9 This product must only be used for nucleic acid amplification Do not use the instrument for any use other than explicitly stated in the User Guide You must use the supplied accessories and tubes Do not modify or delete instrument related information installed within the instrument Operate the LCD touch screen using a non sharp object Nails and other sharp objects may damage the product Do not use powerful detergents or solvents to clean the outside of the instrument as this may cause discoloration If such chemicals are spilled on the instrument immediately clean with a soft cloth Do not keep the instrument in an environment with high humidity Damage from storage in these conditions is classified as water damage and is not covered by warranty Also damage arising from this type of exposure may be irreparable Disassembly and or modification of the instrument voids the warranty and the instrument may be refused service Do not unplug the power cord from the instrument while the instrument is in use This may cause the instrument to malfunction If a burning smell is detected or the instrument seems to be excessively hot during operation immediately stop using the instrument and call your service representative 10 Do not drop or impact the instrument This is a direct cause of instrument damage and may void the warran
2. j Setting USB Disconnected General Figure 30 Editing the instrument name 40 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 2 Network m Set the network settings including IP address for the remote control Setting USB Disconnected General 10 10 100 201 Network 255 255 192 0 10 10 100 1 x r ay ae SS Calibration Figure 31 Network setting menu Touch the values that need to change and the number pad will appear on the center of the screen Insert the values by using number pad Setting USB Disconnected General 10 10 100 201 255 255 192 0 10 10 100 1 x Figure 32 Editing network setting 41 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 3 Data The user can confirm and delete the protocol files and also copy them to USB drive 1 Protocol confirmation a Press Data button and the file manager will display on the right part of screen getting Setting Default ReservedTemplate Imported ON test Reserved Template Test Protocol Test folder Network t X J iii Calibration Delete Figure 33 Data manager screen b When selecting Protocol menu the folders are listed on the center and the files in the corresponding folder are listed on the left screen 42 BQ 042 101 01 www bionee
3. The user cannot create new folder directly from the USB drive in Save Protocol window f there are folders in USB WAllInOneCyclerWProtocols the user can find the folders displayed as USB folder name and select one to save protocols in protocol selection window How to create folders In USB WAllInOneCyclerWProtocols is to create in PC or to copy protocol folders in Data menu of Setting See Copying data to USB drive at p 43 45 30 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block B Edit Prot p Folder USB Default Vv aNew Folder ACS EUOG Test Protocol Figure 21 Save the protocol in USB drive h Press OK button to finish 31 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 3 Opening the existing protocols 1 Open Protocol menu a Press Open Protocol icon at Home menu Open protocol screen will appear ge Open Protocol Folders Preview Protocol Files ReservedTemplate t 40 O 05 00 00 30 00 30 1 00 30 05 00 co _ a Ee eo nm 0 i o zolju Delete From USB Figure 22 Open Protocol screen of the A InOneCycler b Find the previously saved folders in the Folders window on the upper left side of the screen by pressing the up and down arrows And select the protocols at Protocol Files
4. window below Folders window The user can navigate the folders and files with up and down scroll arrow buttons c The corresponding graph of the selected protocol will be displayed on the Preview window at the right of the screen d Atthe bottom of the menu Home Edit Delete and Run icons are displayed 39 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Table 7 Function icons of Open Protocol screen w Delete OE From USB Return to Home menu Edit the selected protocol When pressing this icon Edit protocol screen will be displayed And the user can modify the selected protocol in the same way as in the New Protocol menu Remove the selected protocol The protocols saved in Reserved Template folder cannot be deleted Display the list of the protocols saved in USB drive The user can edit and run the protocols saved in USB drive Start the selected protocol 33 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 2 Open protocols saved in USB drive The user can open or run protocols saved in USB drive a Insert USB drive to the USB ports in front or rear lower of A nOneCycler See figure 2 and 3 at p 13 b A few seconds later From USB icon will be activated ge Open Protocol Folders Preview USB De
5. On screen mouse cursor will appear and the user can operate by clicking sent I gt gt Fail EShow Mouse Calibration Delete Figure 40 Option menu 46 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 5 Functions In Setting menu there are four functions that are described in the following table Table 9 Function icons of Setting menu Se Calibrate the temperature control setting of the Calibration instrument Only for the engineer Save the current settings for each sub menu and save protocol files to USB drive O Delete protocol files or folders Delete Quit Setting menu and return to Home menu The current setting will not be saved without oressing Save icon 7 About menu Displays the current software version of the AlliInOneCycler and the contact information AllInOneCycler S W Version AllinOneCycer 1 00 1 Firmware Version AGC 1 00 0 BiONEER innovation Value Discovery International Korea 82 42 930 8777 1588 9788 Fax 82 42 930 8688 042 930 8688 E mail sales bioneer com sales bioneer co kr Homepage www bioneer com www bioneer co kr Figure 41 About screen 47 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 8 Network Operating A InOneCycler 1 Composition I t is possible to operate multiple AllinOneCycler inst
6. 2041 1 1 and other formats of block are optional Bioneer provides the interchangeable thermal blocks made of 2 different metals normal and novel alloy and classified to 3 sample well types 96 well 384 well and slide PCR The max ramp rate is using AllInOneCycler 96 well PCR system Cat No A 2041 1N When using AllinOneCycler Fast 96 well PCR system Cat No A 2041 1F the max block ramp rates are 9 5 C sec for heating and 7 7 C sec for cooling 12 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 3 System Views Hot top Cover Cover open button Touch TFT LCD Front USB Port Figure 2 Front view of the A InOneCycler Main Power Switch On Off Switch Fuse Box AC Inlet Rear Engineering LAN USB Port Rear USB Port Figure 3 Back view of the A InOneCycler 13 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block V Installing the A ZnOneCycler 1 Site Requirements The AllinOneCycler Thermal Block is for indoor use Ensure that the installation site Meets the spatial and weight requirements Meets environmental requirements ls within 1 5 m 4 92 ft of a 7OOVA power receptacle Is away from water 2 Required Materials Scissors pocket knife or box cutter 3 Unpacking the A InOneCycler caution Save the packing materials and box in case you
7. 5x20mm fuse 5 Place the fuse compartment back into the power entry module and close the fuses door 6 Press the fuses door until it locks in place 7 Connect the instrument power cord 4 Returning an A InOneCycler for Service 1 Decontaminate the instrument A caution If the bath block becomes contaminated with radioactivity use a commercially available decontaminant to remove the contamination If the block cannot be decontaminated the instrument cannot be returned to Bioneer for service 2 Complete instrument decontamination 3 Fax or e mail sales bioneer com the service request form to the customer center 4 Pack the instrument in the provided packaging without any accessories or power cords Include service request form in the box Note Repairs for instruments without the service request form are delayed 5 Affix the provided postage to the box then ship the instrument to the designated facility The repair process requires 1 to 3 weeks 61 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block IX Warranty This instrument 1s warranted by Bioneer against manufacturing defects in materials and workmanship for a limited warranty period of one 1 year from the date you received your product Bioneer will either 1 repair the product at no charge if a hardware defect is found or 2 exchange the product if the same hardware defect arises more than t
8. This will produce condensation which may cause malfunction Be aware of the door moving AN A A This may cause injury 3 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block Electrical Symbols on the A InOneCycler The following table describes the electrical symbols that may be displayed on the AllinOneCycler Indicates the On position of the main power switch Indicates the Off position of the main power switch Indicates a protective grounding terminal in the instruments that must be connected to earth ground l O Indicates a terminal that can receive or supply alternating current or voltage Indicates a terminal that can receive or supply alternating or direct current or voltage Environmental Symbols on the AJInOneCycler The following symbol WEEE applies to the A nOneCycler placed on the European market Do not dispose of A InOneCyc er as unsorted municipal waste Follow local municipal waste ordinances for proper disposal provisions to WZ reduce the environmental impact of waste electrical and electronic lG Instrument gums European Union customers Call your local Europe office for Bioneer instruments pick up and recycling 4 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block lll Safety Warnings and Precautions The warnings and precautions stated below are for the correct and
9. air deliver a blast of air into each well of the bath block to remove any particles that may have collected during transportation 3 Close the hot top cover 2 Connect the power cord to the A InOneCycler 3 Optional Install any of the recommended protective devices 4 Connect the power cord to the 700VA power source receptacle 15 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 5 Power ON 1 Confirm that the hot top cover of the A InOneCycler is closed 2 Press the power button on the back left side of the A InOneCycler Figure 5 Power button of the A InOneCycler 3 When the initialization of the device is completed Home menu will be displayed on screen BiONEER Innovation Value Discovery OG 0 New Protocol Open Protocol Setting About Figure 6 Home screen of the A InOneCycler 16 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block VI Operating the A InOneCycler 1 Loading Samples into the Instrument This section describes how to load samples in tubes or plates See the appropriate section for your instrument There is no need to use mineral oil or glycerin in the block wells or as a vapor barrier over the PCR reaction mixture in the tubes The Tubes fit tightly in the wells and the hot top cover exerts an even pressure on all tubes eliminating condensatio
10. ot wo i o Home Add Delete Open Save Run Figure 24 Selecting a protocol in Edit Protocol screen 35 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 4 Running the PCR protocols Press Run icon on Edit Protocol or Open Protocol screen and then the Run screen is displayed as shown below 1 2 3 6 4 m SS SAE EE 5 12 01 25 36 6 00 02 15 Resume 6 Figure 25 Run screen Table 8 The components of Run screen 1 Protocol Name The name of the current protocol 2 Lid Temperature Show current lid temperature oT The current running step is displayed blinking and the time of the otep step will be decreased The repeat number will be displayed like as current repeat number 4 Repeats full repeat number on Goto step 12 01 25 Show the estimated end time H M S 5 Status messages Show the current block temperature 36 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Description Show the running time H M S 00 02 15 When error occurs on the peltier error messages will be displayed instead of time Return to Home menu Pause is to stop temporarily 6 Function icons Resume is to continue Run again from the Pause state Stop current running protocol When pressing Home icon Home menu will be di
11. safe operation of the instrument Please heed all information for your safety Bioneer Corporation does not assume responsibility for non compliance with the safety warnings and precautions stated below A WARNING Warning Hazards or dangerous actions that may result in severe injury CAUTION Caution Hazards or dangerous actions that may result in minor injury or damage WARNING Warning Hazards or dangerous actions that may result in burn iii DANGER Danger Hazards or dangerous actions that may result in electric shock 1 User and experimental precautions A caution A warning A Dancer 1 Make sure that the power supply 100 240VAC 50 60Hz is correctly connected to the power cord and that the power cord is correctly connected to the instrument Incorrect connection of the power cord can result in instrument damage or failure to turn on 2 This instrument is intended for nucleic acid amplification Please use as such 3 The instrument may stop if the LCD panel is touched while connected to a PC via LAN cable If operating the instrument via PC software please allow for the instrument to finish its orogrammed movement before operating the LCD panel 4 Do not turn the PC off or disconnect the LAN cable connecting the PC to the instrument while instrument is running Data communication error can result in instrument malfunction and can affect the results of your experiment 5 Please in
12. the A InOneCycler 6 occ c ccc ccccc ccc aaaeeeaa aeaaea 38 Or S115 1 A eer een eR T RS ne ee ee eee ee re eee 39 GF AVON ODU serrera e cays coceeseesers eeuapancnatacacs saesremeratarees dass eenensdacede pavaccanal anaes tawasons 47 8 Network Operating A InOneCycler ooo coco ccccccccccccc EEEa 48 VU TROUDISSMOOWING sieri EEEE te eaastucecanase 55 Mill WISE Maimon ano E esiri niriana EN ESERE E E N ERATA 58 1 Cleaning the main body aaannnnnnnnn nunnan nannan aaaeeeaa 58 2 Cleaning the Hot top COVEI wecaescdcere dine ceiensehicendivecuswesebsavediee neidedeesersdiseecauecebeaceaits 59 3 OC IS OS eee oases ate erste en ae Se trae aE NEES ae EEE SERENE EEEE ERARE DEN 59 4 Returning an AllInOneCycler MON DOCS ers ge ceaeee ep case ee EESE EE 61 es VANITY eE O E E EE E 62 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Getting Started Thank you for purchasing this Bioneer product We will try our best to provide satisfactory results to our customers This manual contains practical guidelines and cautions to be taken regarding the instrument Please read this manual carefully and thoroughly before using the instrument Website Please visit us online at http www bioneer com to obtain more information about AllnOneCycl er M General information m AllInOneCycler is a trademark of Bioneer Corporation The information contained in this manual is under c
13. the left side of the Setting screen 1 General In this sub menu the user can set the instrument name and update AllinOneCycler software getting Setting SANEL General a ili i Calibration Delete Figure 28 Setting screen of the A InOneCycler 1 Software update a Before updating software of AllinOneCycler download the recent version from the Bioneer website www bioneer com Once most current software has been downloaded create new folder named USB WAllInOneCycler_Update in USB and then copy the software to the folder b Insert USB drive to the USB ports in front or rear lower of A nOneCycler See figure 2 and 3 at p 13 A few seconds later the message USB Disconnected with red color will change to USB Connected with green color on the right top side of the screen and the S W Update button will be activated 39 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Setting USB Connected i a E General 4 AllInOneCycler Calibration Figure 29 Software update c Press S W Update button and the software will be installed automaticaly and then the instrument will restart 2 Setting the instrument name Touch Instrument Name window and the keypad will appear on the bottom of the screen Rename the instrument name and press return button to apply it
14. 42 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block In USB drive the new folder named AllInOneCycler will be created The protocol files are saved to Protocols folder of AlllnOneCycler If the user selects a folder and oresses the Save icon of the bottom menu the folder will be copied in the Protocols folder s USB Fi a jy AlllnOneCycler Protocols d Reserved Template Test folder Figure 38 Direction of the saved files in the USB drive 3 Deleting protocols For deleting data select file or folder and press Delete icon in the botton menu Then Question message that confirms the deletion of files or folders will appear To finalize deletion press the OK button the success message will then appear setting Setting Do you want to delete Test folder Test protocol Protocol file t K2 Calibration Figure 39 Confirmation message of deleting files 45 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 4 Option This menu is about resetting to factory mode only for the engineer A mouse can be used for this mode 1 Connect USB mouse controller to the front or back USB port lower on AllinOneCycler See figure 2 and 3 at p 13 2 Touch and check the small box next to Show Mouse And then press Save icon of the bottom menu 3
15. Sheet MSDS provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials 5 Minimize contact with chemicals Wear appropriate personal protective Instrument when handling chemicals for example safety glasses gloves or protective clothing 9 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block IV System Components and Specifications 1 System Components Basic Option Option 96 Well Block 384 Well Block Slide Block Accessories Option Option Option Fast 96 Well Block Fast 384 Well Block Fast Slide Block SF a as Wi p Power Cord Option PC Software Figure 1 System components of the A InOneCycler 10 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Table 1 System components of the A InOneCycler AllinOneCycler PCR system Basic A InOneCycler 96 well PCR system Optional AllinOneCycler 384 well PCR system AR04T 2N Optional AllinOneCycler Slide PCR system A RO4T BN A 2041 1F A 2041 2F A 2041 3F A 2041 1 1 A 2041 2 1 Optional A InOneCycler Slide thermal block only A 2041 3 1 AllinOneCycler Fast 96 well thermal block only A 2041 1 2 gt a a AllinOneCycler Fast 384 well thermal block only A 2041 2 2 i I So Optional Optional A 2041 3 2 User s Guide Optional A nOneC
16. User s Guide CE GA 710219 REF A 2041 Series 30 C 15 C Thermal Block BIONEER Innovation e Value Discovery AllinOneCycler Thermal Block AllInOneCycler Thermal Block User s Guide Version No 1 1 2015 11 Please read all the information in booklet before using the instrument Bioneer Corporation 8 11 Munpyeongseo ro Daedeok gu Daejeon 34302 Republic of Korea Tel 82 42 930 8777 Fax 82 42 930 8688 Email sales bioneer com www bioneer com BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block SGS C US 710219 U S and Canadian Safety Standards Standard for Electrical equipment for measurement control and laboratory use Part1 General Requirements UL 61010 1 2 Ed Rev October 28 2008 amp CAN CSA C22 2 No 61010 1 04 R2009 Part 2 Particular Requirements for Automatic and Semi Automatic Laboratory Equipment for Analysis and Other Purposes CAN CSA C22 2 No 61010 2 081 04 Part 2 010 Particular Requirements for Laboratory Equipment for the Heating of Materials CAN CSA C22 2 NO 61010 2 010 04 Part 2 Particular requirements for in vitro diagnostic IVD medical equipment CAN CSA C22 2 No 61010 2 101 04 Bioneer Corporation 8 11 Munpyeongseo ro Daedeok gu Daejeon 34302 Republic of Korea Ec REP MT Promedt Consulting GmbH Altenhofstr 80 D 66386 St Ingbert Germany PRODUCT AllinOneCycler T
17. ce The System is running but there Check if wrong protocol has been used IS an error Check if it is operating after rerunning the same program Request for Service Temperature does not match Instrument may require calibration Request for Service specified temperature Cooling and heating rate too Request for Service slow There is a burning smell in the Disconnect the power and unplug the power connector system immediately 2 Request for Service Hot top cover not heated 1 Request for Service Multi instrument use via PC The PC software does not Inspect the network cables connecting the PC and network operate the instrument hub Inspect the network cables connecting the instruments and network hub Verify that the network hub power is ON Verify the PC network settings Make sure the instrument control software is installed in the correct folder Request for service Instrument is not operating as Check to see if the Run button was pressed more than expected twice Check to see if the software was executed twice Restart the instrument and software Request for service 56 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block No buttons or pictures are 1 Erase and re install the software displayed within the software 57 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block Vill Use
18. ch Run window will appear and then the user can select and run the protocol saved in local PC or the connected instrument E Batch Run Select a Protocol Protocol Files Protocol Preview a LocalProtocols Demo Demo2 LocalMade 1 2 3 4 5 6 7 Incubate Incubate Incubate Incubate Incubate Store Default Select a Target Instrument Instrument Name IP Address AOC96V1N ADA001 10 10 100 201 Figure 48 Selecting a protocol in Batch Run window 5 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block c Select the protocol by using the protocol file tree in the left top side of the screen from the file explorer In this figure the protocol named Demo2 is selected d When a protocol is selected the corresponding protocol graph will be displayed in the right top side of the screen 2 Select a target Instrument a Select the instrument to operate from the list in the lower left of the screen E Batch Run Select a Protocol Protocol Files Protocol Preview LocalProtocols 1 2 4 6 4 Default Select a Target Instrument Instrument Name IP Address AOCS96V1IN ADA001 10 10 100 201 Figure 49 Selecting a target instrument in Batch Run window b Click the check box and then Run button will be activated in the left bottom of the screen 3 Run a Click Run bu
19. connected to the power cord Check if the instrument s power switch is turned off If everything above has been followed but the power is not on contact Service Check if the power was turned off abnormally Remove any residues that may distract operating the system Turn the power on and check whether the system is initiating Request for Service It may be interior problem of the system call for the Service Check if the system is initiating normally when the power iS ON Check if there are any residues or obstacles in the system that may interfere in door closing Check if all the accessories are inserted correctly Check if other buttons in the LCD are working Request for Service Check if there are remaining residues or any obstacles that may interrupt operating the system Check if all the accessories are inserted correctly Request for Service Hold the hot top cover open release the cover and check if cover is closed automatically by the spring Request for Service Check if the pair of magnets attached to the door are correctly attached Request for Service Hot top cover stuck 1 Request for Service 55 BQ 042 101 01 Revision 0 201 2 12 07 www bioneer com AllinOneCycler Thermal Block It stops while operating Check if power supply to the instrument is on Check if the Power switch has been pressed off Check if you have pressed Pause by mistake Request for Servi
20. create a new folder touch the check box at the left of New Folder and assign a name of new folder When New Folder is checked Folder selection window is inactivated 28 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block O RR Edit Prot pees z Default Vv iNew New Folder FileName O oo Figure 17 Creating a new folder e Assign a name to the new protocol BR Edit Prot pas Default V Ww New Folder x ACS Ea Test Protocol 20 O pe ee eee eee Figure 18 Assigning a name of the edited protocol f Ifa folder is not selected the protocol will be saved in the Default folder 29 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block O RR Edit Prot p Folder Default V anew Folder O O Test Protocol EN Jos jog ot g jh ik Figure 19 Default folder setting g When saving protocols in a USB drive insert USB drive in USB ports in the front or rear lower of A InOneCycler See figure 2 and 3 at p 13 And then the user can select USB drive in folder selection window In folder selection window the default name of USB folder is displayed as USB Default and the user can find the saved protocols in USB WAIllInOneCyclerWProtocols s USB CFS a G AlllnOnelCycler G Protocols Figure 20 Direction of the saved files in the USB drive
21. cubate Options i O not use E O Extend time per cycle Sec ira e En Increment temperature ENR E z Ramping Rate C Sec 6o Figure 10 Incubate options of Incubate step option Lower left Touch the button below the temperature and time setting windows The additional three setting windows about Extended time per cycle Increment temperature per cycle and Ramping Rate will be activated Touch the circles in front of each value to edit and then change the values using number pad on the right screen Only one selected option can be applied in a single step 23 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Table 6 How to set incubate options Extended time per cycle Increment temperature per cycle Ramping Rate This menu is to set the gradual time increase and decrease of the selected step with each cycle Touch and change the values between O and 59 Touch B button behind the value to select increase or decrease of time When this option is applied the set value will be displayed on the step of the procedure graph with this noure M This menu is to set the gradual temperature increase and decrease of the selected step with each cycle Touch and change the values between O and 2 0 Touch B button behind the value to select increase or decrease of temperature When this option is applied the set
22. e Store Instrument Name AOC96V1N ADAOO1 IP Address 10 10 100 201 Status Running Protocol Name Demo2 Instrument Run Summary Running a 2 i i J i I u 01 30 00 30 00 30 01 00 Cycle 0 5 01 30 come eo Figure 51 manager exe program window while PCR running 53 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block b If Stop button is clicked the stop signal appears in the screen of the running instrument and then the instrument will stop 1 Step RT PCR 105 C 7 Information Stop PCR by Manager o a 30 00 0500 00 30 _oo 30 00 30 0 30 05 00 G 19 10 46 95T O Home Pause Resume Figure 52 Stop screen of the A InOneCycler controlled by PC c If OK button is clicked the instrument will stop And then the main menu will be displayed on the screen 04 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 Vil Troubleshooting AllinOneCycler Thermal Block The Power is not on The Power is on but the system cannot initiate LCD Screen is not on The system does not operate even if RUN button is pressed The system is running but does not operate correctly The Hot top cover does not close The instrument is not working even if the hot top cover is closed Make sure the power connector is connected Check if the instrument is
23. e SLI CoO OOM eM or aa ceecsen sence eae cancse neue EE E ease cabsau anc TE EEE T 10 2 DOCC CANON S esec cota ce eee A a A E et ewenanecenoneusdecotescr wise TTE 12 Be POV SIC VOWS Sacer e we base nates cueee se beece eine ncsse sence noice sacneseriacemiee needa der secceauescenesepeacerite 13 V Installing the AMINOne Cycler o oo cccccccccsssnsssseseeeeeeccececcccceccceeeeusuaaaaaaaaesseeeeeececececeeseeeuaas 14 Me ee CUI We Mo rsaus ty E EE E cares ueceseedecadesdenesers E yepeeaede ase 14 2 Required VIGTOIVEN S sec oie cuca carn eiswtdantevalawialoax ever eylane mney tania te antiva nation elven exanewnae tuned ada mente 14 3 Unpacking the A InOneCycler resi acdccaccicevciucc sdinevelecwehtwadinecuieedebsesebineiwinawcbicesdiag 14 4 Setting Up the Al InOneCycler ooo ooo coco ccc cc cece cc cece cece cee eee eee ete EEEE EEEE EEEa 15 Oe OS I rena ee eae ee ee a eee eee ee eee ee ee eae earn eee teet as 16 VI Operating the AMINONECYCIer ooo ceccccccsssssssseseeeeeecccceccccccceceeeeauuaaaaaaaeesseesececececcceeeeeeaaas 17 1 Loading Samples into the INStrUMENt 0 0 cece cece eee een teen e eee e beeen teeetenvaeatntnnnnes 17 a FON CMU ce Saree occu men nee eee E EESE AEE E E AA E E eee EE E ane 18 a OWING TIOlO CONS isa tae denen eice de aE RAEE EEr Ee E ARES EAN ne EREE CENTER 19 4A RUNNING the PGR DOLOCOIS sscxc ode ceeswetetncsncaeievaxevecsctecdh sserahstacvecdbiavexckicecveadeisuesenes 36 5 Removing the tubes or plate from
24. f the power plug and insert the plug so that the connection is firm and does not wiggle Incomplete electrical contacts may cause fire Operate the instrument in an ambient temperature range of 15 30 59 86 F Excessive exposure to heat may affect the instrument and yield inexact results Operate the instrument within the recommended humidity range 20 80 no condensation Humid conditions may cause corrosion or malfunction Do not place any objects next to or behind the instrument The instrument may malfunction This instrument contains precision machined parts Do not drop or severely agitate the instrument This can break the instrument and compromise the safety of the product When not using the instrument for a long period of time turn the instrument off and unplug from the wall outlet Overheating and fire may occur Set your PC s Power Options for Turn off the display Turn off the hard disk Standby mode and Sleep mode to Never Because the instrument continuously transmits and receives data from the PC hard disk power down may cause malfunctions Do NOT open the door during instrument s operation as this may result in the instrument to malfunction causing bodily harm including burns to hands or body Moving parts can crush and cut Keep hands clear of moving parts while operating the instrument 6 Precautions and warnings regarding product usage and maintenance caution A warning A vance
25. fault Gradient Fast 2 step Reverse transcription O l 05 00 00 30 00 30 01 00 30 05 00 o0 TT I ir D ga Edit Delete From USB Figure 23 Open the protocols saved in USB drive c Press From USB icon and the list of saved protocols in USB will be displayed in the left side of the screen The USB folders displayed as USB folder name will be listed at the too of Folders window and the folders of AllinOneCycler will be below the USB folder list d The user can select protocols in Protocol Files window and confirm the detailed orocedure of the selected protocols in the right side of the screen 34 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 3 Editing the existing protocol a Press Edit icon in Open protocol menu and the selected protocol will be displayed in Edit Protocol screen b The name of current protocol is displayed on the protocol name on the center of the top screen Press the name and the list Including 8 template protocols with the current protocol will be appeared The user can select one of those for their purposes c Edit details of the protocol See Editing protocols in Edit Protocol screen at p 22 31 Be Edit Protocol 2 Standard 3 step Fast 3 step Fast 2 step Gradient Standard 3 step Gradient Fast 2 step One step RT PCR Reverse transcription Incubation A
26. he steps 3 Step order l The protocol will be sequentially processed Display the temperature of each step 4 Temperature By pressing the temperature icon the user can change temperature values 5 Repeat sections Display the range of repeats 6 Time and Display the time setting of each step or the number of repeats additional information If pressing number the user can change their values Press the scroll arrow buttons and FA to select from long list of 7 Side scroll bar protocols Move to Home menu Add a new step in the right of the selected step Delete the selected step 8 Function icons Open the previously saved protocol file Save the edited protocol Start the current protocol Run 20 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 2 Select a protocol among template protocols We provide 8 protocol template protocols as described below The user can select one of these for their intended purpose Table 4 Template protocol list a Press Protocol name and the list of the template protocols will appear 2 Gradient Standard 3 step Gradient Fast 2 step One step RT PCR Reverse transcription Incubation a Save Figure 8 Selecting a template protocol in Edit Protocol screen b Select from 8 different template protocols in the list c The protocol procedure of the selected template wil
27. hermal Block A 2041 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Contents He Getting Started sa iraeecrpehcigs cane be ce aacinetdecdcaaeuseaaensanencqcnenaeanenonetnnes eteeeesasaeasecanpienecnssasanssacoecmponds 1 I Symbols ON the AMMNONMECYCIEr 0 cece cece cece cence eee e eee e nee e eee ne EE EDAD EEE EG EEE EE EG EE EE EG EEE EE EEE EE EE EEE 3 II Safety Warnings and PreCautions cceccecceceece scene eneeeeee eee eeeeeseneeeseeeeeeeeeeeeesensensenseaeeeees 5 1 Us r and exp rimental DICCAUTIONS srenanencdeearerewamerenet alata teweieueneteuste revaeduerel EdE Deiedra aE 5 2 Precautions regarding the electrical environment eee ee eee eee e eee ee eben eeaennnnes 5 3 Precautions regarding the operation SNVITONMEN wiser cur aveiedovevawayeeevexersbaeresenruveuedaveve ves 6 4 Precautions and warnings regarding Instrument Installation oo 0 cece eee eee eee T 5 Precautions and warnings regarding instrument operation 0 eee eee eee ene e eee eenes T 6 Precautions and warnings regarding product usage and maintenance cece eee ee eee T 7 Precautions regarding moving and lifting instrument eee eee eee eee n eee eneeeeaees 8 8 Warnings regarding biological and chemical hazard safety 00 cece eee eee eee enna 8 IV System Components and Specifications cece cc esceseec eee eeeenseeseeeaeeseeseeseesenseeseeeeueees 10 M
28. hree times during the limited warranty period Any other accessories other than the instrument itself are considered as consumables and warranted for three months Spare parts for the instrument will be available for five years from the initial instrument release date If a defect arises after the limited warranty period shipping and handling charges may apply to any repairs or exchanges of the product undertaken by Bioneer 1 Exclusions and limitations This warranty does not apply 1 Cosmetic damage including but not limited to scratches dents and broken plastic on ports 2 Damages caused by accident abuse misuse flood fire earthquake or other external causes 3 A product or part that has been modified in any way without explicit written consent of Bioneer 4 Damages cause by any services performed by unauthorized engineers or service providers 2 Obtaining Warranty Service Please review this User Manual and access the online support referred to in the manual accompanying this product before requesting warranty service 62 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 service Request Form Product AlliInOneCycler Thermal Block Catalog No A 2041 Serial No Date of request Date of purchase Please list one Service issue or concern per line Issue 1 Issue 2 Service Issue Issue 3 Issue 4 Address Company Customer Name Informat
29. ion Contact Phone Fax E mail Please send service request to Fax 042 930 8688 or E mail sales bioneer com You can download this service request document in Bioneer website www bioneer com Bioneer Worldwide Headquarters Address 8 11 Munpyeongseo ro Daedeok gu Daejeon 34302 Republic of Korea Tel 82 42 930 8777 Korea 1588 9788 Fax 82 42 930 8688 E mail sales bioneer co kr Web http www bioneer co kr Bioneer Inc Address 1301 Marina Village Parkway Suit 110 Alameda CA 94501 USA Tel 1 877 264 4300 Toll Free Fax 1 510 865 0350 E mail order usa bioneer us com Web www bioneer com Bioneer R amp D Center Address 8 702 Korea Bio Park Bldg 700 Daewangpangyo ro Bundang gu Seongnam si Gyeonggi do 13488 Republic of Korea Tel 82 31 628 0500 Fax 82 31 628 0555 E mail sales bioneer co kr Web http www bioneer co kr BIONEER Innovation Value Discovery
30. l This system is compact in size and light weight for easy storage and moving It has user friendly interface with TFT LCD touch screen to allow users to program easily and conveniently AlliInOneCycler Thermal Block also can be controlled with optional PC software which is supplied separately A nOneCyci er is equipped with communication functions between PC and instrument using TCP IP protocol providing full operations from a remote location Users can create PCR protocols on computer that can be loaded onto instrument at a later time Intended purpose Amplification of specific DNA that is included in samples 2 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block ll Symbols on the A 7nOneCycler Symbols on the A ImOneCycl er user manual The following table describes the symbols that may be displayed on AllinOneCycler user manual Conformit Europ enne Mark oN AN IVD n vitro diagnostic medical device Temperature limitation Manufacturer Authorized representative in the European Community a H ES Catalog number Safety Symbols on the A nOneCycler The following table describes the safety symbols that may be displayed on AllinOneCycler Each symbol may appear by itself or with text that explains the relevant hazard Do not touch the surface It is extremely hot Do not operate the bath on at low temperature for a long time
31. l be displayed on screen d Edit the procedure in detail for intended purpose see Editing protocols in Edit Protocol screen at p 22 31 21 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 2 Editing protocols in Edit Protocol screen 1 Modifying the temperature time and cycles of the existing steps a Select the values of the temperature the time and the repeats on the procedure graph b Step Options window will appear There are 4 types of Step Options described in Table 5 c Touch the values that need to be changed d Modify the values by using the number pad on the right side e Press OK button f Step Options window will disappear and the changed values are applied on the current protocol Table 5 Step Options Keeping at a certain temperature for a certain time Setting the range and the number of repeats Keeping at a certain temperature until the user stops the Store orotocols 4 Incubate step ep Options Incubate Options OO wee ae Incubate o B Figure 9 Incubate step option window of existing step 29 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block Temperature range 4 C 99 9 C Time range 1 seconds 5 hours 59 minutes 59 seconds X Set the incubate options additionally Step Options Incubate T T In
32. m Revision 0 2012 12 07 AllinOneCycler Thermal Block AllInOneCycler Server Communication Trace PoplarServer started PortNumber 7890 Client connected IP 10 10 100 201 Recv Login caf89c97 1218 4d98 9749 3afc0343957b AOC9I6V1IN ADADD1 A0C 1 00 0 AllInOneCycler 1 00 1 Send ACK Client connected IP 10 10 100 103 Recv ManagerLogin 6ae257e6 26df 4e29 8647 d809e975be75 Send ACK Recv GetClientList Send ACK Send ClhentList cafS9c97 1218 4d98 9749 3afc0343957b AOCI6V1N ADAO01 10 10 100 201 A0C 1 00 0 AllInOneCycler 1 00 1 Recv ACK Figure 45 Connection status when manager exe program is running in PC f When manager exe is running the currently connected instrument is displayed as Shown below E a AllInOneCycler Manager wel E Select All AOCS6V1N ADA001 IP Address 10 10 100 201 Firmware AOC 1 00 0 Software AllInOneCycler 1 00 1 Refresh Instrument List Pause Resume Stop Run Data Figure 46 manager exe window shows the currently connected instrument 50 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 3 Execution of running 1 Select a protocol a Click the Batch Run icon in the ribbon menu a AlliInOneCycler Manager File View Tools Help AOCSBVIN ADAODOL IP Address a lan 1n dinn ard Figure 47 The ribbon menu of manager exe program b The Bat
33. n Power cord cable should not be repaired with insulating tape Water can still get inside If you use other power cord it should be adequate for the electrical capacity for the AllinOneCycler 250V 16A 0 75 mmf VDE Plugs should match the socket outlets TM r Unplug the power cord from the A InOneCycler when not in operation for a long period of time to prevent the possibility of fire by overheating 10 A InOneCycler is equipped with a 8 conductor AC power cord that when connected to an appropriate AC power outlet grounds earths the instrument To preserve this protection feature do not operate the instrument from an AC power outlet that has no ground earth connection 11 Improper fuses or high voltage supply can damage the instrument wiring system and cause a fire Before turning on the instrument verify that the fuses are properly installed and that the instrument voltage matches the power supply in your laboratory 12 For continued protection against the risk of fire replace fuses only with fuses 250V F10AL 2ea of the type and rating specified for the instrument 3 Precautions regarding the operation environment caution A warning A Dancer 1 2 Avoid placing objects from every which direction the instrument Avoid installing the instrument in a dusty environment Excessive dust may cause malfunction or damage to the Instrument Avoid installation near heat source
34. n Isopropyl alcohol or PCR DNA degradation solution then wipe the cover with a damp cloth 3 Replacing Fuses The instrument has two 250V FIOAL 520mm fuses Required Materials Two 250V F10AL 5X20mm fuses Small screw driver 59 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Procedure 1 Turn off the instrument and disconnect the power cord from the back of the AllinOneCycler PANGER ELECTRICAL SHOCK HAZARD severe electrical shock which could cause physical injury or death can result from working on an instrument when the high voltage power supply is operating To avoid electrical shock disconnect the power supply to the instrument unplug the power cord and wait at least 1 minute before working on the instrument 2 Insert a small screw driver into the slot in the upper portion of the power entry module and open the fuses door Mim Figure 53 The power entry module in the back side of the A InOneCycler 3 Pull out the fuse compartment to inspect the two fuses in the fuse compartment Figure 54 Two fuses in the fuse compartment 4 Pull out the blown fuse s from the fuse compartment and replace with new 250V F10AL 5x 20mm fuses BQ 042 101 01 60 Revision 0 201 2 12 07 www bioneer com AllinOneCycler Thermal Block Figure 55 Pulling out the blown fuse Figure 56 250V F10AL
35. n Protocol Q Setting Create a new protocol View or edit the saved protocols Set the network manage protocol files and calibrate block temperature control The installed software version and the contact information 18 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 3 Editing Protocols 1 Creating a new protocol 1 Press New Protocol icon of Home menu Press New Protocol icon and then Edit Protocol screen will appear In this screen the user can select a protocol among template protocols for new experiment The user can make customized protocols for their intended purpose by changing values such as temperature time repeats and the number of steps in the template orotocol and then save the changed protocol to the desired name on the directed folder CO N 0O ON D D Home Add Delete Open Figure 7 Edit Protocol screen of the A InOneCycler Table 3 The components of Edit Protocol screen Display the name of the pre set protocols and the user can select 1 Protocol name one of those for their purpose After saving the protocol display the name of the saved protocol If this option is checked hot top is used Its temperature fixed at 105 C 2 Lid temperature 19 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block The numbers to distinguish the order of t
36. n on the tubes 1 Loading Samples in A InOneCycler 96 well Thermal Block Loading tubes Prepare 0 2 mI volume PCR tubes Pipette the samples into the tubes 3 Cap the tubes Place the tubes onto the bath block Close the hot top cover Loading the 96 well plate Prepare 0 2 ml volume 96 well plate Pipette the samples into wells of the plate 3 Cover the plates with clear adhesive film Place the 96 well plates onto the bath block Close the hot top cover 2 Loading Samples in the A InOneCycler 384 well Thermal Block Prepare 0 02 ml volume 384 well plate Pipette the samples into wells of the plate 3 Cover the plates with clear adhesive film Place the 384 well plate onto the bath block Close the hot top cover 3 Loading Samples in the A ZnOneCycler Slide Thermal Block Place the slide block on the block guide adapter 2 Pipette the samples onto wells of the gel coated glass 3 Cover the glass with clear silicon 4 Place the slide block onto the bath block 17 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 5 Close the hot top cover 2 Home menu m The user can create a new protocol select the saved protocols change the setup and confirm the information of the instrument selectively on this Home menu Table 2 Function icons of Home menu New Protocol O Ope
37. need to ship the instrument to Bioneer for service 1 To unpack the A nOneCycler Figure 4 Package of the A InOneCycler 1 Cut the straps securing the instrument box 2 Cut the tape securing the top flaps of the instrument crate then open the flaps 14 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 3 Remove the AlliInOneCycler accessories from the instrument and set them aside 4 Lift and remove the cover from the instrument crate 5 Remove the packing material from the AllinOneCycler and then inspect the instrument for shipping damage A caution If the AllinOneCycler is damaged note the location and appearance of the damage and then contact Bioneer Technical Support or your service representative 2 Move the AW nOneCycler to the desired installation site Follow these guidelines for lifting and moving Make sure that you have a secure comfortable grip Keep your spine in a neutral position Bend at the knees and lift with your legs m Do not lift and twist your torso at the same time 3 Open the box containing the AllinOneCycler accessories then verify that it contains AllinOneCycler Thermal Block User Guide Power Cord Power 10A 125V North America or Power 10A 220V Europe 4 Setting Up the AW nOneCycler 1 Remove the packing material 1 Open the A nOneCycler hot top cover 2 Using a can of compressed
38. om Revision 0 201 2 12 07 AllinOneCycler Thermal Block 2 Cleaning the Hot top Cover Clean the hot top cover once a month or as needed Ay WARNING During instrument operation the temperature of the hot top cover can be as high as 110 C and the temperature of the bath block can be as high as 100 C Before cleaning instrument ensure that the hot top cover and bath block reach room temperature To clean the hot top cover Open the hot top cover Soak a cotton swab with pure isopropyl alcohol or PCR DNA degradation solution and gently wipe the heated platen A WARNING CHEMICAL HAZARD Isopropyl alcohol is a flammable liquid and vapor Exposure may cause eye skin and upper respiratory tract Irritation Prolonged or repeated contact may dry skin and cause irritation Exposure may cause central nervous system effects such as drowsiness dizziness and headache Read the MSDS and follow the handling instructions Remove any remaining isopropyl alcohol from the cover Note If the platen becomes contaminated with amplified DNA then raise the hot top cover to the cleaning position wipe the platen with a cloth or cotton swab soaked in bleach about 5 v v concentration in tap water then rinse with water Clean the heated platen once a month or as needed If the hot top cover become contaminated with amplified DNA raise the hot top cover to the cleaning position and wipe the cover with a cloth swab soaked i
39. opyright protection It is unlawful to reproduce part or all of the contents of this manual without the expressed written consent of Bioneer Corporation m Bioneer Corporation reserves the right to alter modify and otherwise make changes to the instrument and manuals without prior notice You must use the supplied accessories and tubes m AllnOneCycler can be used multi system use to PC software Safety Labels Description on the AHInOneCycler English Do not touch the surface It is extremely hot Francais Surface chaude Faites attention a la surface chaude English Do not operate the bath on at low temperature for a long time This will produce condensation which may cause malfunction Francais Ne pas utiliser le bain a basses temp ratures pendant plusieurs heures Risque de condensation pouvant entrainer des fonctionnements d fectueux de l quipement English Be aware of the door moving This may cause injury Francais Manipulation de la porte Risque de blessures corporelles pincement crasement lors de l ouverture fermeture de la porte 1 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block About the A InOneCycler AllinOneCycler Thermal Block is a multifunctional peltier based thermal block equipped with a temperature control system AllinOneCycler Thermal Block has various functions including temperature Increment time increment and ramp rate contro
40. r Maintenance 1 Cleaning the main body Note Power off the A nOneCycler Thermal Block and then remove the power cord Allow the instrument to cool until the hot top cover and bath block reach room temperature 1 Cleaning the Touch screen Clean the touch screen with any commercially available LCD cleaning product Be careful not to scratch the screen 2 Cleaning the Block Wells Do not use other cleaning or decontamination methods other than what is recommended in the manual doing so risks damage to instrument Clean the block wells once weekly or as needed Ay cauTions Always wear protective glasses and gloves when servicing the instrument Also make Sure you disconnect the instrument from AC line power before you begin any service procedure WARNING During instrument operation the temperature of the hot top cover can be as high as 110 C and the temperature of the bath block can be as high as 100 C Before cleaning instrument ensure that the hot top cover and bath block reach room temperature To clean the block wells Open the hot top cover Remove the sample tray from the bath block and set it aside Use a cotton swab soaked in pure isopropyl alcohol or commercial PCR DNA degradation solution to clean the wells thoroughly Make certain that the isopropyl alcohol or PCR DNA degradation solution has evaporated completely before reloading a sample tray 58 BQ 042 101 01 www bioneer c
41. r com Revision 0 201 2 12 07 AllinOneCycler Thermal Block c To confirm protocol press the name of the files twice Details of the selected file will be displayed on screen Protocol Graph View Test Protocol 1 2 05 00 00 30 00 30 00 30 0 30 05 00 Figure 34 Protocol graph view screen 2 Copying protocol files to USB drive a Insert USB drive to the USB ports in front or rear lower of A nOneCycler See figure 2 and 3 onp 13 b A few seconds later the message USB Disconnected with red color will change USB Connected to green color on the right top side of the screen Setting USB Connected wo Default ReservedTemplate General Network Calibration Figure 35 Setting screen when USB drive connected 43 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block c After selecting folder or file by pressing their name press Save icon in the bottom menu If only selecting folder all files in the folder will be saved Setting USB Connected moo General Test protocol Network Test folder t a Calibration Save Delete Figure 36 Selecting a folder and protocol file d The success message Completed will be displayed Setting USB Connected General x Calibration Delete Figure 37 Success message for copying protocol to USB drive 44 BQ 0
42. ruments through TCP IP network The optional PC is necessary in order to control network operation The PC requires Server exe program and Manager exe program for controlling 2 Program operation procedures m For successful program operation it is crucial to follow the procedures described below a Run server exe program in PC b Tray icon of server exe program will appear at the right taskbar AllInOneCycler Server VY i Customize Figure 42 Tray icon of server exe program at right taskbar c The user can view log messages of the server in right click menu of the tray icon View Log Exit KR amp Customize Figure 43 Right click menu of the tray icon of server exe program 48 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block d Turn on the instrument s and Poplar exe operating A nOneCycler will automatically start When turning on the instrument the network between the instrument and PC should be connected Communication Trace Client connected IP 10 10 100 201 Recv Login caf89c97 1218 4d98 97 a9 3afc0343957b AOC9I6V1IN ADAD0N1 AOC 1 00 0 AllInOneCycler 1 00 1 Send ACK Figure 44 Connection status between PC and A InOneCycler e Run manager exe in PC and the connection state will be disolayed as shown below 49 BQ 042 101 01 www bioneer co
43. s This can cause fire Avoid installation near sources of water or damp locations This can cause electrical shock fire or instrument malfunction Do not install near sources of flammable or corrosive gas If there is a gas leak do not touch the power plug but open a window to circulate fresh air Sparks from the power plug Can cause fire and explosions Do not disassemble or modify the instrument in any way This can result in fire electrical shock or malfunction and also voids your warranty 6 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 4 Precautions and warnings regarding instrument installation A caution This is a precision instrument Do not install in a location exposed to direct sunlight Install the instrument on a flat solid surface that is flat and does not move When installing the instrument make sure at least 15 cm separate the instrument from the nearest wall When connecting the PC network hub and A nOneCycler do so with all involved components powered off Connecting components while the power is on may cause damage to the instrument Verify the network cable integrity when connecting between the PC network hub and AllinOneCycler Unstable network connections may result in invalid experimental data from data communication errors 5 Precautions and warnings regarding instrument operation cavrion A DANGER 1 2 Dust of
44. side of this window Step Options Incubate gt elena Incubate Options the oe za o B Figure 13 Step Options window of a new step e In each protocol step the method of inserting values is same to change values that previously described See Modifying the temperature time and cycles of the existing steps at p 22 26 26 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block 3 Deleting steps a Touch the step s to remove Be Edit Protocol 1 2 A D E gt Home Run Figure 14 Selecting a step that needs to remove b Press Delete icon of the bottom menu 4 Opening the previously saved protocol a Press Open icon b Open protocol screen will appear c See Opening the existing protocols p 32 33 in detail 5 Saving the edited protocol a Press Save icon after modifying the protocol in Edit Protocol screen b Save Protocol window will appear 27 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block O RR Edit Prot pe Folder Default Vv eo Figure 15 Save Protocol window c Select an existing folder in Folder selection window Folder New Folder File Name SS Figure 16 Selecting the existing folder to save the edited protocol d Or if it is necessary to
45. splayed with the message blinking PCR Running By pressing it the screen will turn into Run screen again BiONEER BiONEER PCR Running oOGC OmOOO0 O Open Protocol Setting About New Protocol Open Protocol Setting About Figure 26 Home screen during PCR running While running the user can make open and edit protocols at New Protocol and Open Protocol However the current running protocol cannot be edited until the run has ended 37 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Be Edit Protocol 1 2 Delete Open Figure 27 Edit Protocol screen during PCR running If the lid is open the current protocol will be paused Use caution because the lid is still hot 5 Removing the tubes or plate from the A ZInOneCycler Grasp the sample tray retainer assembly or plate firmly with both hands until the tubes or olate are free Sample caps may pop off if the cover is opened when the bath block temperature is above room temperature 38 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 6 Setting menu The instrumental settings and their inspection can be checked in the Setting menu In addition the user can manage the protocol files and folders Press Setting icon at Home menu and the Setting screen will be displayed There are four sub menus on
46. stall the instrument on a flat surface 6 Do not operate the instrument with wet hands as this may result in shock or instrument malfunction Please touch the power cord with dry hands 7 If you make a mistake such as using an improper accessory or force the instrument to stop during a normal operation the instrument may stop working 8 Avoid placing objects in the front and rear of the instrument as fan blockage may cause lowered efficiency of the Cooling Block 9 Be careful not to have any obstructions or foreign materials introduced into the front rear and side Cooling Fan mesh Foreign objects can hinder normal Cooling Fan operation and may lead to overheating of parts or cause a fire 2 Precautions regarding the electrical environment A caution A warning A Dancer 1 If the power cord is loose do not use the instrument Power cord overheating may result in 5 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block shock or fire Do not operate multiole instruments out of a single wall outlet The load may cause overheating and may lead to fire When plugging or unplugging the power cord from a wall outlet make sure your hands are completely dry Wet or moist hands may cause electric shock A convenient and safe power cord should be available The power cord provided by our Company should be used socket outlets should be at least 1 5 m from a sink or wash basi
47. tton the message box confirming whether to run will appear as shown in the figure below b If Yes button is clicked the protocol will be downloaded to the selected instrument and begin 52 BQ 042 101 01 www bioneer com Revision 0 2012 12 07 AllinOneCycler Thermal Block E Batch Run Select a Protocol Protocol Files Protocol Preview 1 2 4 6 z Incubate Incubate Incubate Incubate Incubate Store E Default Confirmation Are you sure you want to start running ae ae Select a Target Instrument Instrument Name IP Address AOC96V1N ADA001 10 10 100 201 Figure 50 Confirmation message of starting the selected protocol in the selected instrument 4 Stop a When the instrument is running Stop button is activated in the lower right of the screen In addition the user can pause or resume the current running PCR by clicking the Pause or Resume button beside the Stop button CT __ AAAA AAA eee ool J AllInOneCyc 0 x File View Tools Help Hi To g hag Run Monitoring Protocol Editor Batch Run Run Monitoring x Instruments Select All AOC96V1N ADA001 IP Address so oo 10 10 100 201 Firmware O1 moco Software Running AllInOneCycler 1 00 1 Refresh Instrument List PCR AOC96V1N ADA001 Instrument Information a 2 3 4 5 6 7 Incubate Incubate Incubate Incubate Goto Incubat
48. ty 7 Precautions regarding moving and lifting instrument A caution 1 The instrument is to be moved and positioned only by the personnel or vendor specified in the applicable site preparation guide If you decide to lift or move the instrument after it has been installed do not attempt to lift or move the instrument without the assistance of others the use of appropriate moving Instrument and proper lifting techniques Improper lifting can cause painful and permanent back Injury Depending on the weight moving or lifting an instrument may require two or more persons 8 Warnings regarding biological and chemical hazard safety A WARNING 1 2 Biological samples such as tissues body fluids and blood of humans and other animals have the potential to transmit infectious diseases Follow all applicable local state provincial and or national regulations Wear appropriate protective eyewear clothing and gloves Before handling any chemicals refer to the Material Safety Data Sheet MSDS provided by the manufacturer and observe all relevant precautions 8 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 3 Always determine what chemicals have been used in the instrument before changing reagents or instrument components Wear appropriate eyewear protective clothing and gloves when working on the instrument 4 Read and understand the Material Safety Data
49. value will be displayed on the step of the procedure graph with this figure Jo0 50 cyde This menu is to set the constant value of the ramping rate Touch and change the values between 0 1 and 6 0 When this option is applied the set value will be displayed on the step of the procedure graph with this cure A 24 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 2 Goto step Step Options Figure 11 Goto step option window of existing step Touch the Goto icon or select number of repeats and Goto Step Options window will appear Change the step number to start the repeat interval The Step Number must be smaller value than the step number in the Goto step Cycles range 1 100 3 Store step Step Options Figure 12 Store step option window of existing step 25 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block Store temperature 4 C 12 C Store step is provided in the last part of each protocol for proper storage of samples 2 Adding new steps a Touch the step to add a new step on their right If a specific step is not selected a new step will be added in front of the first step b Press Add icon of the bottom menu c Step Options window will be appeared d Select a protocol step among Incubate Goto and Store in the top
50. ycler PC control software A 2041 9 x AllInOneCycler 96 well PCR system A 2041 1N is basic model and includes A nOneCycler 96 well thermal block A 2041 1 1 11 BQ 042 101 01 www bioneer com Revision 0 201 2 12 07 AllinOneCycler Thermal Block 2 Specifications Physical specifications 246 mm W x 232 mm H x 369 mm D Dimension 9 68 in W x 9 14 in H x 14 53 in D Weight 8 4 kg 18 52 Ibs Display 7 inch Touch LCD screen 100 240 V Max 8 5 A 50 60 Hz 630 VA 2 USB ports for storage front and lower rear 1 USB port for engineering upper rear 15 C 30 C 59 F 86 P Operating humidity 20 80 no condensation 96 well 0 2 ml 384 well 0 02 ml and Slide PCR type PCR volume range 5 ul 80 ul Operating specifications Temperature range 4 0 C 99 9 C 89 2 F 211 82 F Heating 6 5 C sec 11 7 F sec Cooling 4 5 C sec 8 1 F sec Temperature accuracy EOS Gta OLo4 F Temperature uniformity lt 0 3 C lt 0 54 F Lid temperature 90 C 110 C 194 F 230 F Power consumption Interchangeable block format Maximum ramp rate Temperature increment range 0 1 C 2 0 C 0 18 F 3 6 F Ramp rate control 0 1 C sec 0 18 F sec Upper USB port on the back of the instrument is only for engineer and the user can not use for saving data files t Basic supplied block is A nOneCycler 96 well thermal block Cat No A

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