Research Plus User Manual
Contents
1. Relax the spring by letting the tip cone slide back again above the lower rail Take the tip cone with the cylinder and the spring out of the upper rail Before installation slide the piston into the cylinder Install the channels in reverse order Assembling the multi channel pipette Boom Fit the cover plate with integrated ejector Push the latches upwards To mount push the lower part into the upper part until it engages audibly Carry out a gravimetric test of the systematic and random error see Technical data on p 23 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 19 33ueu3jute A Maintenance 20 Eppendorf Research plus Operating Manual 5 Maintenance 55 Calibrating pipettes P Caution Incorrect dispensing volume for special liquids and from temperature differences Solutions whose physical data deviate significantly from those of water and differences in temperature between pipettes pipette tips and liquids can result in inaccurate dispensing volumes gt Check the dispensing volume gt Avoid differences in temperature 5 5 1 General notes The Research plus was calibrated tested und fitted with a silver calibration seal before delivery The calibration display on the side reads 0 A calibration of the Research plus is only required for solutions which are very different from water with regard to thei2. 1 1 Features The Research plus pipettes are piston stroke pipettes which function according to the air cushion principle When the control button is pressed the piston in the pipette moves in the same direction If the control button and the piston are moved upwards the liquid can be aspirated into the pipette tip With the downward movement of the piston the liquid is dispensed measuring stroke Blow out occurs if the downward piston stroke exceeds the first stop Depending on the Research plus pipette it is possible to dispense volumes from 0 1 uL to 10 mL The Research plus family consists of single channel and multi channel pipettes 8 and 12 channels with variable volume settings as well as single channel fixed volume pipettes You can find a list of all available models of the Research plus in the Technical Data section All Research plus pipettes are fully autoclavable You can calibrate the Research plus pipettes The side viewing window displays the change in the calibration When attaching a pipette tip the spring loading action of the tip cone is activated exceptions 5 mL and 10 mL pipettes 1 2 Delivery package 3 The Research plus delivery package contains Pcs Description 1 Research plus operating manual 1 Certificate 1 Adjustment tool allen key with blue handle 1 Mini CD 1 Black locking ring for single channel pipettes lt 1 000 uL 5 Red calibration seal 5
3. Eppendorf Research plus Operating Manual eppendorf Eppendorf Research plus Operating Manual Copyright 2008 Eppendorf AG Hamburg No part of this publication may be reproduced without the prior permission of the copyright owner Trademarks eppendorf and Research are registered trademarks of Eppendorf AG Hamburg Germany Registered trademarks are not marked in all cases with or 9 in this manual U S Pat No 5 531 131 4 961 350 For user adjustment with calibration display spring to reduce force for tip fitting and for further features patents are pending Further information can be found on the enclosed CD Research plus Operating Manual en 01 0309 Eppendorf Research plus Operating Manual Table of contents Product description 4 151 E 7 10 ERECTAE 4 1 2 Delivery package ir ie peer a Rude Race RI E o cede eade ac Re 4 h s EU MUSA ONE er da ake An i alates tn tei echt Egger un don a eek Rn 5 1 3 4 Research plus single channel eee eee 5 1 3 2 Research plus multi channel eene 6 Safely ex RE AR whe ERR Exe SR ew Dies NEE EEN RE RA LEE ee EE 7 2 1 Warning signs and hazard icons eee 7 22 intended Usos sax atte vx eben eres bx a ac did 7 2 3 Hazards arising from the intended use 7 Operation nese pd ere 9 3 1 Setting the volume adjustable volume pipettes Research plus only 9 8 2 Attaching pipette tips ee cete ehe eh Rm ODORE E EP e e ded 9 3 3
4. 1 000 pL 0 6 1 2 0 2 0 4 250 uL blue 50 1 000 uL t 0 6 15 0 2 0 5 500 uL blue 50 1 000 pL 0 6 3 0 2 1 1 000 uL blue 50 1 000 uL 0 6 6 0 2 2 Test conditions according to ISO 8655 1 8655 2 and 8655 6 for piston stroke pipettes with air cushion by means of a metrologically tested fine balance with evaporation protection Number of determinations 10 water according to ISO 3696 20 C to 25 C 0 5 C constant with pre wetted pipette tip dispensing against the tube wall Eppendorf Research plus Operating Manual 6 Technical data 6 4 Ambient conditions Temperature range Relative humidity Storage without 5 to 45 C 10 to 9596 transport packaging Operating 5 to 40 C 10 to 95 conditions Technical specifications subject to change Note Ordering information can be found on the enclosed CD 25 ejep jeoruuaal e Technical data 26 Eppendorf Research plus Operating Manual 6 Technical data Eppendorf offices AUSTRALIA amp NEW ZEALAND AUSTRIA I BRAZIL 2 Eppendorf South Pacific Pty Ltd Phone 61 2 9889 5000 Fax 461 2 9889 5111 E mail Info eppendorf com au Internet www eppendorf com au Eppendorf Austria Phone 443 1 29017560 Fax 43 1 290175620 E mail office eppendorf at Internet www eppendorf at Eppendorf do Brasil Ltda Phone 455 11 30 95 93 44 Fax 55 11 30 95 93 40 E mail ep
5. 165 mm 10 mL t 0 6 60 0 15 15 0 e Technical data 24 Eppendorf Research plus Operating Manual 6 Technical data 6 2 Research plus multi channel adjustable volume Model 8 or Step size Color code epT I P S Volume Error 12 channels Volume range Systematic Random volume Length 96 Zu Ce Zut range 0 5 10uL 0 01 uL medium gray 0 5 uL 12 0 06 8 0 0 04 0 1 20 uL 1uL 80 008 50 0 05 40 mm 5uL 40 0 2 2 0 0 1 10 uL 2 0 0 2 1 0 0 1 10 100 uL 0 1 uL yellow 10 uL 30 0 3 20 02 2 200 uL 50 uL LO 0 5 0 8 0 4 53 mm 100 uL t 0 8 t 0 8 t 0 3 t 0 3 30 300 uL 0 2 uL orange 30 uL 3 0 0 9 1 0 0 3 20 300 pL 150uL 1 0 t15 05 0 75 55 mm 300 uL t 0 6 t1 8 t 0 3 t0 9 63 R h plus fi l 6 esearch plus fixed volume Model 7 epT I P S color code Error volume Volume range Systematic Random t tuL 96 t uL 10 uL medium gray 0 1 20 uL 12 0 12 0 6 0 06 20 uL light gray 0 5 20 uL L 0 8 0 16 0 3 0 06 10 uL yellow 2 200 uL 12 t 0 12 0 6 0 06 20 uL yellow 2 200 uL t1 0 0 2 0 3 0 06 25 uL yellow 2 200 uL 1 0 0 25 0 3 0 08 50 uL yellow 2 200 uL 0 7 0 35 0 3 0 15 100 uL yellow 2 200 uL 0 6 0 6 0 2 0 2 200 uL yellow 2 200 uL t 0 6 t 1 2 0 2 0 4 200 uL blue 50
6. Aspiratinig e 0 EEN 10 3 4 Dispensing liquids Kaca esee ke x ed aces t Sh K Roc E ced 10 Troubleshootirig 55 rh RE ERREUR Ra Koh NEEN 11 4 Errorsearc usse o t eee DS b RE e Rb NER LEE E 11 Maintenance eere sd gia din b j kms RS Rx Rm Re RR Rum UR EUR UR Bon ROS t 12 bal GJENIN One c rrt E aPOD Pr LESE demens e e Y Su UE E 12 5 2 Sterilizing or disinfecting the pipette aa 12 5 2 1 Autoclaving cse eee ees CR ea eem m ego pa AUS 12 RTE ele WEE 13 5 3 Replacing O rihgs zer does ER ER t pr bs d Ae K kd RE EE EE 14 5 8 4 Remove Omg 14 5 3 2 Mounting a new Omg 14 5 4 Research plus disassembly and assembly 15 5 4 1 Single channel up to 1 000 uL adjustable and fixed volume 15 5 4 2 Single channel 5 mL and momt 0 0 c eee eee 17 5 43 Multi channel AN EE RR eR REIR REESE 18 5 5 Calibrating pipettes e erre uen e uxore UR EN ue d 20 5 5 1 Generalnotes os osse e ura ORE REA REA de e Rx 20 5 5 2 ProGcedure pra esee eere Ee aeu e E Ra P P EES 21 5 6 Decontamination before shipment eee 22 Technical data Se aciem segue I E ene eRe deed EE er ee 23 6 1 Research plus single channel adjustable volume 23 6 2 Research plus multi channel adjustable volume 24 6 3 Research plus fixed volume eee eee 24 6 4 Ambient conditions EE 25 sjuajuo2 JO 3 qe Product description Eppendorf Research plus Operating Manual 1 Product description
7. still lying with the lever facing downwards Take off the cover panel with integrated ejector rail Do not remove the ejector rail from the cover panel The spring for the ejector rail could accidentally come off and get lost Eppendorf Research plus Operating Manual 5 Maintenance Removing and installing channels Channels must only be removed and installed if the multi channel lovver part is separated from the upper parti The channels in the lovver parts consist of piston cylinder and spring The channels for 100 uL and 300 pL are equipped with an O ring at the tip cone see Replacing O rings on p 14 e T n i T T i d Ale DA J DA J TIA IT Wil I d E 2 Heese Gel en J EE ESL E gj t o Ef gj Q and Position a pipette tip under the piston and carefully take the piston off the upper rail Carefully pull the piston out in an upward direction Do not bend the piston Take the tip cone at the lower end and push it slightly upwards This compresses the spring QO Lift the tip cone slightly and release it from the lower rail
8. the use of epT I P S uondiosoap Pnpold Product description 1 3 2 Eppendorf Research plus Operating Manual 1 Product description Research plus multi channel For an explanation of the pipette upper part please refer to the full illustration of the single channel pipette see p 5 1 Lever To release the lower part of the multi channel pipette 2 Multi channel lower part The multi channel lower part can be rotated freely The lower part is not released when it is rotated The outer channels are marked with the numbers 1 and 8 and or 12 The multi channel version has a piston for each channel so that it can be fitted with less than 8 and or 12 tips The lower part can be exchanged from an 8 channel to a 12 channel version with an identical volume range The multi channel lower part can be opened so that individual channels can be replaced or removed 3 Latches right and left Releases the cover plate with interior ejector rail 4 Spring loaded tip cones Spring loading optimizes the force for taking up and ejecting the tip 5 Pipette tips We recommend the use of epT I P S 6 Cover plate Removable cover plate with interior ejector rail Eppendorf Research plus Operating Manual 2 1 Warning signs and hazard icons Depiction Meaning A WARNING LIN VVarning of potential injury CAUTION Not
9. tip s completely press the control button until the I second stop blow out 4 Hold control button and wipe the tip s against the inner tube wall 5 Letthe control button slide back slowly outside of the tube 6 To eject the tips press the ejector Pipette tips are for single use only Eppendorf Research plus Operating Manual 4 Troubleshooting 4 1 Error search Observation Possible cause Error correction Liquid is dripping from the tip and or dispensed volume is incorrect Pipette tip is loose or does not fit correctly gt Attach tip firmly use epT I P S If using 5 mL and 10 mL epDualfilter T I P S do not use protection filters in the pipette Liquid with high vapor pressure and or different density gt Wet tip several times and calibrate pipette for the liquid used Pipetted too quickly gt Move control button slowly Tip withdrawn from the liquid too quickly gt Withdraw tip from the liquid slowly and with a time delay of about 3 seconds Liquid aspirated with blow out or dispensed with blow out gt Repeat procedure and dispense correctly Piston soiled or damaged gt Clean piston relubricate slightly and or replace Tip cone damaged gt Replace lower part or channel O rings of the tip cones are damaged gt Replace O rings 100 pL 300 uL multi channel only Control button jam
10. to the figure on the next page 17 33ueu3jute A Maintenance 18 Eppendorf Research plus Operating Manual 5 Maintenance 4 Hold the piston 4 which is now partly visible in the lower part 5 Push the piston mount 1 slightly downwards 3 mm and turn it by 45 The piston spring pushes the piston mount upwards The lower part opens The lower part consists of the following Ej Se po 3 4 5 6 Piston mount 5 mL 10 mL 2 Piston spring 3 Upper part of the lower part 4 Piston with seal 5 Cylinder and tip cone 6 Protection filter in the tip cone Before assembling the pipette install the piston mount the piston spring and the piston in the upper part of the lower part first Further assembly is as shown in the figures Carry out the procedure in reverse order After the assembly gt Carry out a gravimetric test of the systematic and random error see Technical data on p 23 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 5 4 3 Multi channel Loosening and opening the multi channel lower part 4 X0 and Slide the lever on the lower part to the left or right This separates the lower part from the upper part so that it can be removed Put down the lower part with the lever facing downwards G Slide the two latches down Use a coin to do this The lower part is
11. volumes 10 UL Is the fine balance sufficiently sensitive balance resolution 0 001 mg Eppendorf Research plus Operating Manual 5 Maintenance Were original epT I P S pipette tips used for testing Information as to which pipette tip must be used as a test tip for checking the technical data can be found in the Technical Data chapter of the operating manual Only if you can answer all the questions with yes may the calibration be changed 5 5 2 Procedure If the calibration is changed the volume changes by a certain value Strictly speaking this change only applies to the testing volume Example You recalibrate a 10 100 uL pipette with a volume setting of 100 uL by 1 uL 1 uL s 1 96 If the volume setting is 10 uL the pipette is also adjusted by 1 uL 10 96 eppendort Ra a eppendort Re AA BEE m Remove the silver calibration seal Keep the ejector pressed Insert the adjustment tool from the delivery package Turn the adjustment tool until the desired value is displayed on the calibration display Carry out weighings to verify trueness and precision 6 After the checks stick the red calibration seal from the delivery package on If the calibration is meant for a specific liquid mark the pipette accordingly Use the labeling area on the pipette for this purpose and write down the liquid and the volume Check any changes to the calibration gravimetrical
12. Protective filters for 5 mL and 10 mL pipettes 1 Tool for opening the lower part 5 mL and 10 mL Sample epT I P S or box exception fixed volume pipettes 1 Tool for cutting the O rings multi channel 100 pL and 300 uL only Eppendorf Research plus Operating Manual 1 Product description 1 3 Full illustration 1 3 1 Research plus single channel 1 Control button The control button and the trays of the matching epT I P S pipette tips are the same color 2 Volume adjustment To set the volume for adjustable volume pipettes 3 Ejector Moves the ejector sleeve and ejects the pipette tip 4 Volume display adjustable volume pipettes only Read the set volume from top to bottom 5 Adjustment opening The adjustment opening is covered by the silver calibration seal when the pipette is delivered 6 Calibration display Set to 0 when pipettes are delivered 7 Labeling area Space for adhesive label with internal lab data The serial number is located at the bottom 8 Ejector sleeve Discard the pipette tips after use 9 Spring loaded tip cone Spring loading optimizes the force for taking up and ejecting the tip not spring loaded for 5 mL and 10 mL pipettes The tip cone of the 5 mL and 10 mL pipettes contains an easily exchangeable protection filter 10 Pipette tip The Research plus pipettes can only be used with matching pipette tips We recommend
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14. dispensing if it is safe to do so gt With all dispensing tasks make sure that you are not endangering yourself or anyone else Caution Damage to the device from missing pipette tips or penetration of liquid gt Use the Research plus with fitted pipette tips only gt When using standard tips without filter use the 1 10 mL and 0 5 5 mL pipettes only with the prorection filter inserted gt Do not put the pipette down if the tip still contains liquid N Kyayes Safety Eppendorf Research plus Operating Manual Caution Material damage and dispensing errors caused by faulty use or cleaning gt Use the pipette tips only once gt Do not clean the pipette with acetone or aggressive solutions gt Do not use use any sharp edged objects for cleaning gt epTt 1 P S are made of PP epDualfilter T I P S are made of PP and filters are made of PE Do not use the pipette and pipette tips with liquids which corrode these materials The parts of the Research plus which might come into contact with liquid if handled or cleaned incorrectly are made of PP PEI PVDF PC PPS silicone stainless steel gt Do not autoclave the epDualfilter T I P S gt If filter tips are used use the 1 10 mL and 0 5 5 mL pipettes without protection filters The protection filter and the filter in the tip can interfere with each other which can lead to tip leakage Caution Lack of safety due to missing op
15. erating manual gt gt When passing on the device always enclose the operating manual If you lose the operating manual request a replacement You can also find the current version of the operating manual on our website www eppendorf com Eppendorf Research plus Operating Manual 3 Operation 331 Setting the volume adjustable volume pipettes Research plus only gt To change the volume turn the volume setting as shown The height of the control button changes as the volume is adjusted The numbers on the volume display are to be read from the top to the bottom The decimal places are below the hyphen Up to 1 000 uL the volume is displayed in uL For the 1 and 10 mL sizes the volume is displayed in mL We recommend to adjust the volume setting from a higher value to a lower value If required turn beyond the required value and then back again 7 The pipette is only functional if a pipette tip has been mounted gt If you are using pipette tips without filters insert the protective filters into the 5 mL and 10 mL pipettes gt If you are using the 5 mL or 10 mL epDualfilter T I P S remove the protective filter from the pipette first This also applies to 5 mL 10 mL filter tips of other manufacturers The filters can interfere with each other The backpressure of the two filters makes it difficult to exactly identify the first stop see p 10 gt Fit suitable pipette tip s on the t
16. hat the two stop pins engage in their retainers Installing the lower part and ejector sleeve Jg U U 1 Insert the lovver part into the upper part until it engages audibly 2 Set the maximum volume and operate the control button several times It must run smoothly and resistance free 3 Keep the ejector pressed The ejector rod protrudes from the upper part 4 Fitthe ejector sleeve onto the ejector rod It is fitted correctly if it engages slightly 5 Carry out a gravimetric test of the Systematic and random error see Technical data on p 23 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly Eppendorf Research plus Operating Manual 5 Maintenance 5 4 2 Single channel 5 mL and 10 mL Replace the protection filter in the tip cone after each contact with liquid or if it is frayed E cppendore kes 1 Unscrew the ejector sleeve 2 QO Keep the ejector pressed and turn the lower part to the left or right by approximately 30 The lower part is automatically released from the upper part 3 Opening the lower part Position the key delivery package on the lower part Hold the cylinder and unscrew it from the lower part For the next steps please refer
17. he delivery package 1 Push the black locking ring onto the lower part from the top 2 Insert the lower part into the upper part until it engages audibly 3 Keep the ejector pressed The ejector rod protrudes from the upper part 4 Fitthe ejector sleeve onto the ejector rod It is fitted correctly if it engages slightly Remove the locking ring to reactivate the spring loading action 15 33ueu3jute A Maintenance 16 Eppendorf Research plus Operating Manual 5 Maintenance Opening and assembling the lower part 1 000 pL 1 To avoid confusion of parts only disassemble J and assemble one pipette at a time 2 Opening the lovver part 1 On the piston mount 1 push the stop pins together slightly 2 Remove the piston mount 3 Remove piston 2 and piston spring 3 3 s The piston is under spring tension Pistons and piston springs will look different for the individual volume 1 versions Assembling the lovver part KS Te 1 Guide piston and piston spring into the cylinder Make sure that the piston is guided correctly in the cylinder and in the PusHuPTo PUSHUPTO PUSHUPTOI piston spring There must not be any PEN pw perceptible resistance In the case of piston springs with double windings 4 these windings must point downwards 2 Keep piston and piston spring pressed 3 Keep the stop pins on the piston mount pressed with the other hand 4 Mount the piston mount in such a way t
18. ification of lovver risk or danger of material damage 2 2 Intended use The Research plus is a lab device intended for dispensing liquids in the volume range from 0 1 uL to 10 mL in combination with matching pipette tips In vivo applications applications in or on the human body are not permitted The Research plus may only be operated by trained specialist staff All users must have read the operating manual carefully and familiarized themselves with the device s mode of operation 2 3 Hazards arising from the intended use Warning Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than those recommended by Eppendorf may impair the security function and precision of the device Eppendorf accepts no warranty or liability for damage caused by third party parts or incorrect use gt Use only original accessories recommended by Eppendorf Warning Damage to health from toxic radioactive or aggressive chemicals as well as infectious or pathogenic samples gt Follow national regulations governing the handling of these substances gt Wear personal protective equipment PPE at all times when working with hazardous biological or chemical substances and use protective equipment Warning Danger to persons from careless use gt Never point the tip cone of a Research plus equipped with a pipette tip at yourself or anyone else Only initiate
19. ip cone applying light pressure The pipette tip sits firmly on the tip cone when it responds with spring loaded action exception no spring loading action for 5 mL and 10 mL pipettes You can deactivate the spring loading of the tip cone when using single channel pipettes See p 15 uoye1sdg Operation 10 Eppendorf Research plus Operating Manual 3 Operation 3 3 Aspirating liquid t Provide the liquid in a suitable tube For multi channel pipettes we recommend the reagent reservoir Tip Tub 2 Press the control button until the first stop measuring stroke 3 Immerse pipette tip s vertically 4 mm into the liquid 4 For liquid aspiration let the control button slide back slowly Maintain the immersion depth so that no air is aspirated accidentally 5 For large volumes Wait for 3 seconds before pulling the pipette out of the liquid For highest precision and trueness we recommend wetting each new tip first by aspiring and dispensing the liquid one to three times Only then should pipetting commence 6 Remove the tip s slowly from the liquid 7 Wipe the tip s carefully against the tube wall to ensure that there is no outer wetting on the tip s 3 4 Dispensing liquids 1 Apply tip s to the tube wall at an angle I 2 Press the control button slovvly until the first stop measuring stroke and wait until the flow of liquid stops 3 To empty the
20. isinfectants decontamination agents or sodium hypochlorite during autoclaving or UV exposure gt When autoclaving make sure that the temperature does not exceed 121 C gt Before using disinfecting agents or decontaminating agents test for material compatibility and check the manufacturer s instructions about chemical resistance Also pay attention to the materials of the pipette 5 2 1 Autoclaving All Research plus pipettes are fully steam autoclavable Eppendorf Research plus Operating Manual 5 Maintenance 5 2 2 Before autoclaving 1 Remove any contamination from the inside and outside of the lower part see Research plus disassembly and assembly on p 15 2 f you remove any grease only use the grease specified in the ordering information see enclosed CD for slightly relubricating the piston sealing ring 5 2 3 Execution Follow the operating manual of the autoclave manufacturer 1 Autoclave at 121 C 20 minutes 1 bar overpressure 2 When placing the Research plus into the autoclave make sure that the temperature at the pipette does not exceed 121 C 3 You can put the Research plus into the autoclave as a whole unit or with the lower part removed Do not disassemble the lower part 4 5mLand 10 mL pipettes Remove old protection filter Have the new protection filter available and insert it after autoclaving Autoclave the protection filter only once 5 If the lower parts have been removed make
21. low so that the sharp edge in the opening of the tool and the O ring are positioned on top of one another Do not slide the tool onto the cone 2 Supporting the tool with your thumb push it firmly against the tip cone from below The O ring is cut in one place 3 Remove the tool and the O ring from the tip cone 5 3 2 Mounting a new O ring 1 Fitthe mounting aid shortened pipette tip on the tip cone 2 Push the new O ring over the tip onto to the tip cone 3 Check that the pipette is functioning Eppendorf Research plus Operating Manual 5 Maintenance 5 4 Research plus disassembly and assembly 5 4 1 Single channel up to 1 000 pL adjustable and fixed volume Removing the lower part 1 Keep the ejector pressed and pull off the ejector sleeve 2 and On the lower part slide the ring marked PUSH UP TO RELEASE up by about 5 mm until the lower part comes off 3 Take the lower part out of the upper part Deactivating the spring loading action installing the locking ring For dispensing tasks in which apipette tip is used over a long time or the pipette tip is slightly bended it can be helpful to deactivate the spring loading of the tip cone This can also be helpful for pipette tips which are not designed for the Eppendorf pipette tip cone The spring loading of the tip cone can be deactivated by installing the locking ring The locking ring is included in t
22. ly Please note the test procedures of EN ISO 8655 2 and 8655 6 An SOP Standard Operation Procedure can be found on our website www eppendorf com Qi p cuc 21 33ueu3jute A Maintenance 22 Eppendorf Research plus Operating Manual 5 Maintenance 5 6 Decontamination before shipment If you wish to return the pipette to Eppendorf AG or an Eppendorf AG service partner for checking or repair please note the follovving Cautioni Risk to health from contaminated pipette 1 Perform decontamination before returning or storing the pipette 2 The pipette must be clean and free of hazardous substances Hazardous substances are solutions presenting a hazard to health potentially infectious agents organic solvents and reagents radioactive substances proteins presenting a hazard to health DNA 1 Please note the information in the document Decontamination certificate for product returns You can find it as a PDF file on our homepage www eppendorf com 2 Enter the serial number of the Research plus into the decontamination certificate This can be found at the lower end of the entry field 3 Please include with the shipment the completed and signed Decontamination certificate for product returns for each pipette Eppendorf Research plus Operating Manual 6 Technical data ejep jeoruuaal 6 1 Research plus single channel adjus
23. pendorf eppendorf com br Internet www eppendorf com br CANADA P CZECH REP amp SLOVAKIA D Eppendorf Canada Ltd Phone 1 905 826 5525 Fax 1 905 826 5424 E mail canada eppendorf com Internet www eppendorfna com Eppendorf China Ltd Phone 86 21 68760880 Fax 86 21 50815371 E mail market info eppendorf cn Internet www eppendorf cn Eppendorf Czech amp Slovakia s r o Phone 420 323 605 454 Fax 420 323 605 454 E mail eppendorf eppendorf cz Internet www eppendorf cz www eppendorf sk FRANCE INDIA 2 Eppendorf France S A R L Phone 33 1 30 15 67 40 Fax 33 1 30 15 67 45 E mail eppendorf eppendorf fr Internet www eppendorf fr Eppendorf Vertrieb Deutschland GmbH Phone 449 2232 418 0 Fax 49 2232 418 155 E mail vertrieo eppendorf de Internet www eppendorf de Eppendorf India Limited Phone 91 44 42 11 13 14 Fax 91 44 42 18 74 05 E mail info eppendorf co in Internet www eppendorf co in ITALY NORDIC Eppendorf s r l Phone 390 2 55 404 1 Fax 390 2 58 013 438 E mail eppendorfGeppendort it Internet www eppendorf it Eppendorf Co Ltd Phone 81 3 5825 2363 Fax 481 3 5825 2365 E mail info eppendorf jp Internet www eppendorf jp Eppendorf Nordic Aps Phone 445 70 22 2970 Fax 45 45 76 7370 E mail nordic eppendorf dk Internet www eppendorf dk SOUTH amp SOUTHEAST ASIA SWITZERLAND Eppendorf Asia Pa
24. r density viscosity surface tension and or vapor pressure If the density of an aqueous solution changes by approximately 10 for example because of the salt concentration the volume changes by approximately 0 2 This statement does not apply if other relevant properties of the liquid also change If the pipette is used at extremely high altitudes it must be calibrated to the ambient air pressure At 1 000 m above sea level the volume error is about 0 4 The actual volume can be checked by weighing Mean value of the weighings Actual volume SE Se Density liquids at vveighing temperature If the set volume corresponds to the actual volume no correction is necessary The density of distilled water is approx 0 9982 mg uL at 20 C If there is a difference between the actual volume and the set volume please check the following Is no liquid dripping from the tip Is the pipette tip fitted leak proof s the tip cone undamaged Are piston and cylinder leak proof Does the temperature of the pipetted liquid correspond to the temperature of the device the ambient air temperature s the weighing location free from drafts Does the work method and pipetting speed permit complete aspiration and dispensing of the liquid Has the correct numerical value for Density of the liquid at weighing temperature been used for the calculation of the actual volume s the set volume correct For very small
25. s runs jerkily Piston soiled Seal soiled Pipette blocked gt Clean lower part gt 5mL and 10 mL sizes replace protection filter Calibration seal removed calibration display changed Pipette has been calibrated for another liquid gt Calibrate pipette for the liquid used see Procedure on p 21 No spring loading action of the tip cone when taking up pipette tips Spring loading action is blocked by locking ring gt Remove locking ring again You are using a 5 mL or 10 mL pipette No remedy available The tip cones of these Sizes do not have spring loading action Regularly check precision and trueness of the Research plus to avoid dispensing errors You can use the PICASO software from version 2 3 for determining the maximum permissible Systematic and random error If the pipette is used at an extremely high location it must be calibrated to the ambient air pressure You can find an SOP Standard Operation Procedure for the test on our website www eppendorf com 11 Bunoousa qnou Maintenance 12 Eppendorf Research plus Operating Manual 5 Maintenance Cautioni Irreversible damage due to aggressive liquids Vievving vvindovvs surfaces and print vvill be damaged if aggressive liquids are used The parts of the pipette which might come into contact with liquid are made of the following materials PP PEI PVDF PC PPS silicone and
26. stainless steel Further information can be found on the enclosed CD gt Pay attention to the materials of the pipette and accessories gt Do not clean the pipette with acetone or other aggressive liquids gt Do not allow any liquids to penetrate the upper part of the pipette All single and multichannel lower parts are wear parts Clean them after contamination use of aggressive chemicals and or heavy stress If the lower parts are worn or damaged replace the respective parts Faulty dispensing results are sometimes due to lack of maintenance 5 1 Cleaning Remove contaminations on the outside of the Research plus as follows gt Wet a cloth with a mild cleaning fluid and water and remove the contamination Caution Damage from sharp objects The cylinder can get damaged from sharp objects gt Do not use sharp objects for cleaning gt To remove heavy contamination resulting from ingress of fluids disassemble the lower part of the pipette see Research plus disassembly and assembly on p 15 gt Use demin water for cleaning Relubricate the piston sealing rings after contamination use of aggressive chemicals and or heavy stress Remove the old grease before relubricating Use only the grease specified in the ordering information see enclosed CD 5 2 Sterilizing or disinfecting the pipette Caution Irreversible damage from incorrect treatment gt Do not use any additional d
27. sure that no lower parts are confused during reassembly Tip Use one plastic beaker per pipette 5 2 4 After autoclaving gt Cool pipette down to room temperature and leave to dry gt 5mL 10 mL pipettes The protection filter swells during autoclaving Slightly compress the protection filter when inserting it into the tip cone The piston does not need to be relubricated after autoclaving If autoclaving is carried out as described above no gravimetric testing or calibration is necessary 5 2 5 Disinfecting gt Wipe off the outer surfaces with disinfectant DNA RNA decontamination agent or 70 96 isopropanol gt Alternatively wipe the outside of the tip cone and ejector sleeve with 4 sodium hypochlorite solution and leave on for a while gt After the exposure time of the sodium hypochlorite solution has elapsed remove thoroughly with demineralized water The Research plus pipettes may be temporarily subjected to the UV light of a sterile bench 2254 nm 13 33ueu3jute A Maintenance 14 Eppendorf Research plus Operating Manual 5 Maintenance 53 Replacing O rings The multi channel lower parts 100 uL and 300 uL are equipped with O rings These are wear parts Replace old worn or damaged O rings Defective O rings result in incorrect positioning of the tips and in dispensing errors 5 3 1 Remove O ring 1 Push the opening of the tool delivery package against the tip cone from be
28. table volume Model Step size epT I P S Color code Volume Error volume range Volume range Systematic Random Length tel ul 0 1 2 5 uL 0 002uL dark gray 0 1 ul 48 10 048 12 0 012 0 1 10 uL 0 25uL t12 Iron 6 0 015 34 mm 125gL x2 5 20 031 t 1 5 0 019 2 5 uL 1 4 0 035 0 7 0 018 0 5 10uL 0 01uL medium gray O5ul 8 0 04 25 0 025 0 1 20 uL 1 uL 25 0 025 1 8 0 018 40 mm 5 ul t1 5 0 075 0 8 0 04 10 uL t1 0 t 0 1 t0 4 0 04 2 20 uL 0 02 UL light gray 2 uL 5 0 1 t1 5 0 03 0 5 20 UL 10 uL t1 2 0 12 120 6 0 06 46 mm 20 uL t1 0 t 0 2 0 3 0 06 2 20 uL 0 02 uL yellovv 2 uL tb t 0 1 t1 5 0 03 2 200 uL 10pL 12 0 12 06 40 06 53 mm 20 uL 1 0 0 2 0 3 0 06 10 100 uL 0 1 uL yellow 10uL 3 03 1 0 1 2 200 uL 50uL 1 05 03 0 15 53 mm 100uL 0 8 t 0 8 0 2 0 2 20 200 uL 0 2 uL yellow 20gL 25 05 0 7 0 14 2 200 uL 100uL 1 1 03 120 3 53 mm 200 uL 120 6 1 2 0 2 0 4 30 300 uL 0 2 uL orange 30 uL t2 5 0 75 t0 7 X 0 21 20 300 uL 150uL 1 21 5 0 3 0 45 55 mm 300 UL 120 6 1 8 0 2 0 6 100 1 000 uL 1 uL blue 100gL 3 3 0 6 0 6 50 1 000 pL 500uL 1 5 t0 2 121 0 71mm 1 000 uL 0 6 t6 t0 2 2 0 0 5 5 mL 0 005 mL Jpurple 05mL 2 4 12 0 6 3 0 0 1 5 mL 25mL 1 2 30 0 25 126 0 120 mm 5 mL t 0 6 30 0 15 8 0 1 10 mL 0 01 mL turquoise 1 ml 3 30 0 6 6 0 1 10 mL 5 mL t 0 8 40 0 2 10 0
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