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440 Fraction Collector

1. Initial move_t0_A Initial move_tO x a hoo hoo Set Point 16 8 113 5 Clear X and Y Offsets Clear X and Y Offsets SetPointB 366 8 113 5 Move To Point A Move To Point A Set Point C 266 8 213 5 Capture Point A Capture Point A SetPoint D 266 8 13 5 Move To Point B Move To Point B Capture Point B Capture Point B Calibrate Calibrate Calibration Check Calibration Check Move To Point C Move To Point C Move To Point D Move To Point D Ensure the last movement of Z is a downward stroke Use number keys to move the Autosampler NumLock must be on Use the left pull down menu to change the magnitude of the movement in mm l2m_xtra am ytrans move_rad move t0 eny 90 eny 180 env t 270 fo fo Jo fo fo fo fo Upper Plane l2m_xtrans_A l2m_ytrans_ move_rad_4 moe WA env 90A env t 180A env t 270_A boo b b b bh LowerPlane Reset_z Close Figure 48 Clearing the Upper Level Calibration Coefficients 2 Place the alignment tool on top of the sample rack tray holder in such a way that the top 2 feet they will have a pointy bottom slip into the two holes in the tray Figure 49 Once the alignment tool is in place it should look like the one in Figure 50 Figure 49 Holes in the Plate to Calibrate Point A 78 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual tA Figure 50 Alignment Tool in Position to Calibrate Poin 3 Select the M
2. 5 3 Component Installation and Replacement Procedures Information is provided on the following Installing the low delay volume tubing Replacing the probe and probe tubing Determining the volume delay time Determining the probe depth Replacing the tubing Replacing the fuses Warning Chemical Hazard Inhalation Hazard Fire Hazard Tubing may contain residual chemicals which may be harmful if you come in contact with them Some solvents may have flammable properties To avoid injury or damage to equipment consult the MSDS and always follow appropriate safety guidelines when using chemicals and solvents and always wear appropriate safety equipment and clothing NA ds VARIAN N OARA N Where Figure 13 Fraction collector components Publication no 8510253500 Issue 1 August 2009 a Or a GN Theta axis Z axis slide Probe Rack Rack location mat Retaining ring Probe mounting block Knurled mount nut on the probe assembly Ferrule holding the metal probe 35 of 88 Varian 440 Fraction Collector Service Manual 5 4 Installing the Low Delay Volume Tubing Caution Use this tubing for operation of the fraction collector at or below 1 mL min or collection of very narrow lt 30 seconds wide peaks to assure good results This kit should not be used when operating at flows of 2 mL min or higher Warning Chemical Hazard Inhalation Hazard Fire Hazard Tub
3. 8510253500 Issue 1 August 2009 Received DKI JI Figure 41 Varian MDS Software 9 50 AM Port 1 Open 73 of 88 Varian 440 Fraction Collector Service Manual 74 of 88 5 Inthe diagnostics window Figure 42 click on the Calibration button U Diagnostics Com 1 9600 AIM3000 mode Varian ELO7073734 Main Board Communication Relay PIR Firmware V1 20 PR C2 07 eS Copyright C Ai Scientific 2007 Digital Read D0000000 Pos Read 40 44 23 57 0 00 Stp Read 916 891 0 Mat Dump 0 Accessories Address Firmware C Generic Board C Intemal Diluter Internal Pump Query Variables au Address Firmware C Generic Board Internal Diluter Internal Pump Query Variables Extemal Diluter _ Enc Read 916 891 0 X P Move Z __Move XY _ 2 5 i 3 0 aay ie General Communication Send Cancel i 1 RESET ResetZ Gene Move XY MoveZ Variables Firmware Open Log Send Script Options stepper_read Reply 916 891 0 Main Board Settings Jumper Read Represents 8 Not Used High or On 7 Not Used mm Represents 6 Not Used Low or Off 5 Not Used 4 Not Used 3 Probe Monitor Relay 2 AIM3300 Defaults Loaded at reset 1 4IM3200 Defaults Loaded at reset DIP Read File Loops Done Test Mode swi swz T Test Mode Noma 2 Test Mode ext a Test 2 3 Reserved Test 3 4 Reserved 5 Baud Rate On 19 2k Off 9600 6 On AIM1250
4. Page 33 Replace the probe See Section 5 5 Page 37 Valve is sticking Replace the valve Contact your Varian representative Probe hits the top of The probe depth is not Set the probe depth See Section 5 7 Page 41 the tubes or is not low set correctly 62 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Symptom Possible Cause Suggested Remedy The wrong rack is Choose the correct rack in System Parameters or the 440 chosen LC Console software See the 900 LC Series HPLC Help or the 440 LC Fraction Collector Console software Help tips The wrong vials are Replace with the correct vials being used The arm is not Motor not operating Run the Reset procedure See Section 7 1 Page 63 moving correctly in correctly Run the Self test procedure See Section 7 2 Page 64 the X theta or Z axes Contact your Varian representative if a test fails The fraction collector Fraction collector is Turn the fraction collector on shows offline in turned off Check the power cable connections Status Overview Check the fuses See Section 5 9 Page 42 Fraction collector is on Check the RS232 cable connections but not communicating Reboot the fraction collector Troubleshoot communication See Section 7 5 Page 65 Check the LEDs See Section 7 3 Page 64 The green indicator Indicator may be faulty Turn off the fraction collector Wait 10 seconds before on the front of the turning
5. o ft a aa i Pe Varian 440 Fraction Collector Service Manual 74 Probe Arm Jam A probe arm jam is indicated when the fraction collector cannot complete its instructed move This could be due to The probe arm hitting an obstacle while moving into position The probe arm not reaching a position within the defined limits of the intended position This causes the probe movement to cease Movement errors of less than 1 mm are enough to cause a probe jam e The mechanical movement being abnormally stiff making it difficult for the probe arm to move The probe drive will cease In all cases if an error occurs the error indicator LEDs will show the error code and an error message will be sent to the computer If the probe arm becomes jammed the error control system will lock the motor drive and send an error message to the computer and to the error indicator LEDs If this happens re initialize the fraction collector power off wait 10 seconds power on and then watch for correct initialization If the probe line has been squashed as a result of the probe arm jam the tubing should be replaced to prevent low sample flow or carryover 7 5 Communications This LED flashes when serial communications from the client computer are received Use the computer to send software commands to the fraction collector If the LED flashes the internal computer is operating and it is receiving commands from the HPLC system computer and
6. stpr zemor band f8 eny 90 45 45 45 45 odom_z_steps env_t_180 env_t_270 sys_stx sys_conf_chksum sys_etx sys_prog_chksum PIR Firmware 0000105154 aip_a_baud 7 Boundary Variables sys ver hip_a_echo sys_id move min move 2 min model sys_dilutor 1 move_x_max 385 000000 move_z_max move_y_min Place the mouse pointer over the variable name for a description of the variable a and what units are used Refer to the Programmers Manual before adjusting variables Green DEM Variables move_y_max 227 000000 Red Advanced DEM Variables Me Read Variables d Save As Print Figure 35 Writing the Calibration to the Firmware sp_mode 0 12 Press the Close button again Figure 36 and you will be back to the main page of the software Figure 37 U Diagnostics Com 1 9600 AIM3000 mode Varian ELO7073734 Main Board Communication PIR Firmware Y1 20 PR C2 07 Copyright C Ai Scientific 2007 Digital Read Doonan 8 Pos Read 40 44 23 57 0 00 Stp Read 916 891 0 Enc Read 916 891 0 Move Z Move KY f Mat Dump Rely A EA E i si RESET Reset Z Move XY MoveZ 0 0 Accessories Address Firmware C Generic Board C Intemal Diluter Internal Pump Query Variables E Address Firmware C Generic Board Internal Diluter Internal Pump Query Variables Extemal Diluter 70 of 88 M General Communication Figure 36 Closing the Diagnostics
7. the housing _ Figure 17 Retaining ring on underside of housing 7 Disassemble the probe by unscrewing the ferrule holding the metal probe from the bottom of the knurled mount and then remove the lower knurled mount nut that retains the knurled mount 38 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Where 1 Knurled mount on the probe assembly 2 Lower knurled mount nut on the probe assembly 3 Ferrule holding the metal probe ee Figure 18 Probe assembly shown disassembled 8 9 Lift the probe and tubing out of the Z axis slide Pull the tubing out of the probe if you are replacing the tubing 10 The Tefzel tubing is secured by the black tubing You will need to fit the black tubing back over the Tefzel tubing and metal probe once the new tubing has been installed To install the sample probe and probe tubing 1 Slide the 1 16 inch OD x 0 20 inch ID Tefzel or the 1 16 inch OD x 0 010 inch ID for the low delay volume kit tubing into the stainless steel probe if you are replacing the tubing inside the probe 2 Push the tubing down into the probe so that at least 0 5 cm tubing protrudes from the end of the probe 3 Slide the probe into the probe mounting block 4 Fit and tighten the knurled nut holding the probe to the knurled mount 5 Fit and tighten the ferrule to the knurled mount 6 Clip the tubing int
8. 1 LED Status E Reserved Reserved Digital Status 2000001010 Represents High or ON 1 8 I Represents Low or OFF MRS Expiry Timer ON 4min OFF 30s ON MRS OFF Fixed Wash Refresh Manufacture Diagnostics Exit Baud Rate ON 19 2K OFF 9600 ON 4IM1250 OFF AIM3000 Mode Sent aux_read Received OK I Port 1 Open 11 09 2008 3 50 AM Figure 29 Varian MDS software SADA WN gt 4 Click on the Diagnostics button at the bottom of the screen U Diagnostics Com 1 9600 AIM3000 mode Varian EL07073734 Main Board Communication General Communication z Relay B Variabl PIR Firmware 1 20 PR C2 07 ariables Copyright C Ai Scientific 2007 Send Canca Firmware Digital Read DODDODDI i i 1 8 RESET Reset Z _Opentog _Send Script Ortons e Acc Firmware Pos Read 11 63 10 72 0 00 Sent stepper read e eee Stp Read 391 962 0 Move xY Move Z Reply 391 962 0 P j Enc Read_ 391 961 0 U Main Board Settings Moez Jumper Read Represents DIP Read Tee Se Move XY 8 Not Used High or On 1 Test Mode e 7 Not Used am Represents 2TestMode Test Test 2 6 Not Used 3 Reserved ae a 5 Not Used Ee ET 4 Reserved IS 4 Not Used 5 Baud Rate On 19 2k Off 9600 3 Probe Monitor Relay 6 On AlM1250 Off AIM3000 Mode 2 AIMS300 Defaults Loaded at reset 7 TYR Expiry Timer On 4min Off 30s 1 AIM3200 Defaults Loaded at reset 8 On TAR Off Fixed Wash Reservoir Accessories Address
9. 46 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Figure 24 PCB cable connected 5 13 Remove and install the x axis encoder PCB 5 13 1 Remove the x axis encoder PCB 1 Remove the rear cover 2 Disconnect the x axis FFC and motor cable 3 Remove the two M3x6 screws and star washers 4 Carefully remove x axis encoder PCB so as not to damage the encoder wheel 5 13 2 Install the x axis encoder PCB 1 Locate the x axis encoder PCB on the x axis motor bracket Ensure that the encoder wheel can turn freely through the encoder sensor mounted to the PCB 2 Secure the x axis encoder PCB to the x axis motor bracket using two M3x6 Screws 3 Install and check the encoder wheel alignment 4 Connect the x axis FFC and motor cable 5 Install the rear cover 5 14 Remove and install the theta axis PCB 5 14 1 Remove the theta axis PCB 1 2 3 Remove the x axis cover Disconnect the x axis FFC cable from the theta axis PCB Unclip the ferrite from theta axis PCB taking care not to damage the FFC cable Disconnect the theta axis stepper motor cable from the theta axis PCB Disconnect the z axis FFC cable from the theta axis PCB Turn the each of four M4x10 self tapping screws securing the theta axis PCB to the x axis carriage a half a turn Do not remove these screws Taking care not to damage the encoder wheel or encoder slowly slide the theta axis PCB from t
10. 6 If unsuccessful contact your Varian representative Publication no 8510253500 Issue 1 August 2009 65 of 88 Varian 440 Fraction Collector Service Manual 7 6 77 7 1 1 66 of 88 Failure to Initialize The fraction collector initialization process is a powerful self diagnostic tool The initialization procedure is as follows On power up the fraction collector runs through the initialization sequence and sets the probe position The probe moves to all the physical limits of the working envelope of the fraction collector and then returns to the home position at the drain This initialization sequence will be performed each time the fraction collector is switched on The probe travels through the following sequence e Probe raises to the full extreme of the Z axis e Probe travels to the full extremes of the X axis e Probe travels to the full extremes of the Theta axis e Probe positions itself at the back left of the fraction collector If the fraction collector fails to initialize the probe arm has not travelled the required distance along one or all of the axes One or more LEDs will light to indicate an error In this section detail diagnostic and troubleshooting procedures relevant to the instrument model For example There are two tools that can be used to troubleshoot the Microplate Reader e The diagnostic LEDs on the Microplate Reader main board e The communications program called HyperTerminal that is
11. M z 6 Not Used f 3 Reserved Eeee un 5 Not Used Lera 4 Reserved Teas 4 Not Used 5 Baud Rate On 19 2k Off 9600 3 Probe Monitor Relay 6 On AlM1250 Off AIM3000 Mode 2 AIM3300 Defaults Loaded at reset 7 TAR Expiry Timer On 4min Off 30s 1 AIM3200 Defaults Loaded at reset 8 On TAR Off Fixed Wash Reservoir Accessories Address Firmware Generic Board Internal Diluter Internal Pump Query Variables Hi Address Firmware Generic Board Internal Diluter Internal Pump Query Variables Extemal Diluter Figure 32 Diagnostics Section of the SPS Software 68 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 9 Select the Variables option Figure 32 and that should open the Calibration Variables Page U Variables Com 1 9600 AIM3000 mode Varian EL07073734 Motion Variables r Calibration Variables stpr_x_vi move_rad move_rad_ 121 013924 stpr_x_vf stpr_zd_vf A 2m_xtrans 2m_xtrans_A stpr_x_a stpr_zd_a l2m_ptrans 12m ytrans A stpr_x_j stpr_2d_j 2m_theta l2m_theta_A stprt_vi stpr_zu_vi move t0 move WA stpr_t_vf stpr_zu_vf move_pi move pi A stpr_ta stpr_zu_a move_tr fa 000000 move WA 290 000000 stprtj stpr_zu_j move tps jor 45160 move_tps_A move_xmo move_xmo_A 25 000000 odom_x_steps Mmove_mpsx move_mpsx_A move_mpsz j move mpsz A env_t_90 env_t_30_A
12. Off AIM3000 Mode 7 DAR Expiry Timer On 4min Off 30s 8 On TAR Off Fixed Wash Reservoir Figure 42 Diagnostics Screen of the Varian MDS Software 6 Once the calibration window Figure 42 opens up Clear the X and Y Offsets on the Lower Plane Calibration U Calibration Com 1 9600 AIM3000 mode Varian Lower Plane Calibration Initial move DA 10 0 lear X and Y Offsets gt T Move To Point A Capture Point A Move To Point B Capture Point B Calibrate Calibration Check Move To Point C Move To Point D Ensure the last movement of Z is a downward stroke Move_z po l2m_xtrans l2m_ytrans b Wp p Upper Plane Calibration Initial move_t0 10 0 Clear Xx and Y Offsets Move To Point amp Capture Point A Move To Point B Capture Point B Calibrate Calibration Check Move To Point C Move To Point D Increment 5 v move rad move t0 l2m_xtrans_4 l2m_ytrans_4 move_rad_ move IDA eo fpf Calibration Points A and B must lie on the same Y value m 113 5 1135 2135 Set Point Set Point B Set Point C Set Point D Use number keys to move the Autosampler NumLock must be on Use the left pull down menu to change the magnitude of the movement in mm env_t_90 env_t180 env 270 pb bpo O pb Upper Plane env 90A env t1804 env_t_270_A o booo booo Lower Plane Reset_z Close Figure 43 Clearing the X and Y Offsets Publication no 8510253500 Issue 1
13. Settings lente Tee OOOO EEC EEC GOOG ELEG Start Action 1 Rack 1 Tube 1 v Probe Movement Linear Serpentine Divert Valve between moves Drain vi Rack Position 1 After last tube Next Rack Rack Position 2 After last tube C Next Rack Rack Position 3 After last tube Stop 5055 53605 85055 OO800 Factory Defaults Upload 28 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 3 3 Determining the Volume Delay and Probe Depth You will need to determine the volume delay the length of time it takes for the liquid to go from the outlet of the detector to the probe Using the volume delay option will help compensate for the extra time required for the sample to reach the collection tube You will also need to determine the correct probe depth for the probe to travel across the racks without interference See Section 5 6 and 5 7 Page 42 for instructions on how to determine the volume delay and probe depth Publication no 8510253500 Issue 1 August 2009 29 of 88 Varian 440 Fraction Collector Service Manual This page is intentionally left blank 30 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 4 Operation The Varian 440 LC Fraction Collector can be used in three different configurations e With an existing or new modular system and Galaxie softw
14. The Spare Parts section of this manual Section 6 contains a comprehensive ordering information list of spares and their part numbers Where possible pictures are used to illustrate a part for visual identification Electronic and mechanical schematics including layouts and assembly drawings are included in the Schematics section of the manual Section 8 Service manual updates will be provided by Varian when necessary and will come in the form of Service Bulletins or Service News Flashes or Service How To sheets Publication no 8510253500 Issue 1 August 2009 1 of 88 Varian 440 Fraction Collector Service Manual 1 3 1 4 1 5 1 6 ls 1 7 1 2 of 88 Conventions The following conventions have been used throughout the documentation e Menus menu options and field names e g select Copy from the Edit menu have been typed in bold Bold is also used to signify the pushbuttons appearing throughout the software e g click OK e ALL CAPITALS indicate keyboard and mouse commands e g press the F2 key and text you must type in from the keyboard e g type SETUP at the prompt Tips A Tip is used to give practical hints to help you achieve the best possible performance from your instrument General This section provides an overview of the fraction collector and contains important information about e Design features e Safety practices and hazards Design Features The fraction collector is manufact
15. Y Offsets Set Point B Move To Point A Move To Point A Set Point C Capture Point Capture Point Set Point D Move To Point B Move To Point B Capture Point B Capture Point B Calibration Check Move To Point C Move To Point C Move To Point D Move To Point D Ensure the last movement of Z is a downward stroke Use number keys to move the Autosampler NumLock 5 gt must be on Use the left pull down menu to change the Move_z 50 Increment 5 magnitude of the movement in mm l2m_xtrans l2m_ytrans move rad move t0 eny 90 eny ti80 eny t 270 lo lo 0 lo 0 0 0 Upper Plane l2m_xtrans_4 2m_ytrans_4 move_rad_ A mo WA eny t90 A envy t 180A env_t270LA bp fb pf b f Loep Reset_z Close Figure 47 Calibrating and Checking the Calibration 13 Proceed to the upper level Calibration 7 7 3 2 Fraction Collector Calibration Upper Level Plane This portion of the Calibration requires the alignment tool SPS 3 Alignment kit P N 99 101216 00 Makes sure that you have completed the Lower Level Plane calibration before proceeding to the upper level calibration 1 Clear the upper plane calibration X and Y offset Clear X and Y Offset Figure 48 Publication no 8510253500 Issue 1 August 2009 77 of 88 Varian 440 Fraction Collector Service Manual U Calibration Com 1 9600 AIM3000 mode Varian Lower Plane Calibration Upper Plane Calibration Calibration Points 4 and B must lie on the same Y value
16. and that it is not damaged If necessary replace the fuse s in the holder 5 Ensure each fuse is held securely by the prongs inside the holder If the contact is too loose remove the fuse from the holder and then use a pair of pliers to compress the distance between the prongs so as to tighten their grip on the fuse Replace the fuse 7 Prongs Figure 21 Fuse holder 6 Slide the fuse compartment back into the compartment and clip the cover back into place ensure that it clicks into the locked position 7 Reconnect the power supply cable and switch on power to the unit Publication no 8510253500 Issue 1 August 2009 43 of 88 Varian 440 Fraction Collector Service Manual 5 10 Removal and installation of covers 5 10 1 Rear panel Remove the rear panel as follows 1 Remove the eight M3x6 screws and M3 star washers the secure the rear panel to the column case 2 Remove and lay the rear panel on the work area taking care not to stress the rear panel ground strap 3 If necessary remove the M4x12 screw that secures the ground strap to the rear panel and place the rear panel in a safe area Install the rear panel as follows 1 Secure the rear panel ground strap to the rear panel with the M4 x 12 Screw and two M4 star washers Order star washer ground strap star washer M4 x 12 screw 2 Secure the rear panel to the column case using the eight M3x6 screws and M3 star washers 5 10 2 Probe arm assemb
17. depth height that will lower the probe into the tube The probe height should move the probe down to just above the tubes The probe is not raised up before moving to the next tube Instructions are provided for the Galaxie software and 440 LC Console software To determine the probe depth using the Galaxie software 1 Turn on the Galaxie Software your HPLC system and the Varian 440 LC Fraction Collector 2 Select the appropriate system in the Galaxie software 3 Scroll to the Fraction Collector area in the System Parameters tab in the Device Settings window 4 Click in the Probe Depth field to edit the value and then press ENTER on your keyboard or use the arrow buttons to move the probe up or down The probe will move to Rack X position 1 and move to the depth specified X is the rack number of which of the Probe Depth is being edited 5 Repeat steps 4 5 until the correct probe depth has been determined 6 Enter in the Probe Depth to the remaining positions if you are using the same tubes for each rack position 7 Repeat steps 4 6 for the remaining positions if you are using the different tubes for each rack position To determine the probe depth when using the 440 LC Console software 1 Turn on the 440 LC Console software your HPLC system modules and the Varian 440 LC Fraction Collector 2 Select the appropriate COM port and then click Connect in the Console software Publication no 8510253500 Issue 1
18. is 842 mm for the 440 fraction collector cut the timing belt to suit if required 2 Fit the timing belt over the drive pulley on the x axis stepper motor the feed the timing belt through the slot in the chassis column and over the tensioning idler pulley 3 Clamp the ends of the timing belt together three teeth from each end with the belt clamp 4 Secure the belt clamp to the x axis carriage with the two M4x10 self tapping screws 5 Allow the spring to apply tension to the timing belt Re tighten the M3x6 screw 6 Slide the x axis carriage along the x axis shafts several times to ensure smooth movement 7 Install the probe arm front cover 8 Install the x axis cover 5 12 Removal and installation of PCBs Caution Before handling printed circuit board assemblies ensure that the proper precautions are taken to electrically ground technicians and equipment This is to prevent potential damage to the printed circuit board assemblies by static discharge 5 12 1 FFC cable connectors Bridge retainer Figure 23 PCB cable connector 1 Carefully open the bridge retainer of the connector by sliding forward of the connector and lifting up see Figure 5 2 Gently slide the FFC cable fully into the connector ensuring the cable is central to the connector and with the blue stripe on the cable showing 3 Pull the bridge retainer of the connector down and slide back into the connector to lock the cable into position
19. laboratory use To reduce the risks of electrical shock this equipment employs a three wire electrical cord and plug to connect the equipment to earth ground To preserve this safety feature e Make sure the matching wall outlet receptacle is properly wired and earth grounded to provide a protective earth Never use e A three wire to two wire plug adapter e A two wire extension cord e Non grounding multiple outlet receptacle power boards Publication no 8510253500 Issue 1 August 2009 3 of 88 Varian 440 Fraction Collector Service Manual 1 7 3 1 7 4 1 7 5 1 7 6 4 of 88 Ensure to position the fraction collector so that it is easy to access the mains power switch or to remove the power cord Any servicing of this equipment which requires removal of covers or panels can expose parts which involve the risk of electric shock or personal injury Refer such servicing to trained qualified personnel Fire Hazards To ensure safety against fire the fraction collector is protected by fuses that provide multiple levels of protection against over current conditions e The unit is protected by a power inlet fuse e The internal electrical circuits are protected by fuses e The internal fuses are not user replaceable and are not accessible to the user These can only be replaced by an Authorized Service Agent and e The power inlet fuse is a user replaceable fuse For continued protection against risk of fire
20. lo fo fo fo fo fo Upper Plane l2m_xtrans_4 l2m_ytrans_4 move_rad_A move WA env t 90A env_t_180_A env_t_270_A boo ko b b b bo p y lme Resetz Close Figure 52 Capturing Point B 5 Locate the holes in the front side of the fraction collector rack holder tray Figure 53 and move the alignment tool in such a way that the front pointy feet are now in the second set of holes shown in Figure 53 Once the alignment tool is positioned it should look like Figure 54 Figure 53 Alignment holes on sample tray 80 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Figure 54 Alignment Tool Position in the Tray for B Point Calibration 6 Move the probe to position B Move To Point B Figure 55 and follow the same alignment process as mentioned for the position A calibration Once you are happy with the probe alignment over position B Figure 55 in the alignment tool click the Capture Point B button Figure 52 Click the Calibrate button Figure 52 to lock in the new calibration coordinates There is no need to check Point C and D Figure 55 Calibration Point B in the Alignment Tool 7 7 3 3 Calibration Test To test the new calibration coordinates remove the assy probe 0 8mm and re attach the fraction collector probe Once you have successfully attached the fraction collector probe go back to the main page of the Varian MDS software and follow the procedure belo
21. lower knurled mount nut and then the lower knurled mount nut from the top knurled mount nut Where 1 Knurled mount on the probe assembly 2 Lower knurled mount nut on the probe assembly 3 Ferrule holding the metal probe i a Figure 3 Close up view of the probe disassembled 5 Insert the probe into the probe mounting block See Figure 1 Page 13 Publication no 8510253500 Issue 1 August 2009 15 of 88 Varian 440 Fraction Collector Service Manual 6 Secure the lower knurled mount nutto the top knurled mount nut 7 Secure the nut holding the metal probe into the bottom of the lower knurled mount nut 8 Clip the tubing into the tube restraint which is the small hook at the top of the Z axis slider Allow for a small length in the tubing to prevent kinking the tubing 9 Run the tubing to the left around the probe arm housing 10 Attach the split ring also called the retaining ring as shown below The retaining ring is split to attach it through the loop on the underside of the probe arm housing 11 Feed the tubing through the retaining loop attached to the underside of the housing See the image below for more information ee Figure 4 Retaining loop on underside of the probe arm housing 12 Screw the tubing fitting into the bottom port 3 of the valve To waste From detector outlet or back pressure restrictor To the probe Figure 5 Fraction collector valve Note 16 of 88 It
22. no 8510253500 Issue 1 August 2009 49 of 88 Varian 440 Fraction Collector Service Manual 517 5 17 1 5 17 2 5 18 5 18 1 50 of 88 oN Secure the main PCB in position with three M3 x 12mm screws and M3 star washers refer to the circles on Figure 8 for mounting locations Insert the six connector fasteners through the case to the connector shrouds and fasten Connect the x axis and theta axis FFC Connect the power cable Connect the accessories cable if fitted Install rear panel Note After installation of a new main PCB recalibration of the fraction collector is necessary Remove and install the accessories PCB Remove the accessories PCB 1 2 3 4 5 Install the 1 OF OV Be Go Remove the rear panel Disconnect the accessories cable from the accessories PCB Disconnect the daisy chain accessories cable if fitted Remove the four M3 x 12mm screws and M3 star washers that secure the accessories PCB Carefully remove the accessories PCB and discard accessories PCB Move the accessories PCB as required aligning the four mounting holes with the PCB spacers Secure the accessories PCB in position with four M3 x 12 mm screws and M3 star washers Connect the accessories cable to the accessories PCB Connect the daisy chain accessories cable if fitted Check that the address on the board is correct Install the rear panel Remove and install the x axis stepper motor Remove the x axis st
23. number 0390793814 This will convert the 9 pin D connector to an RJ 45 which is then plugged into the serial ports on the back of the MIB x i i t A 5 Figure 9 Serial ports on the MIB800 or MIB850 3 1 2 4 Connecting the 440 LC Fraction Collector to a Modular HPLC System without Galaxie Software Use the wiring diagrams in figure 10 and 11 as an example of how to connect your Varian 440 LC Fraction Collector Additional cables and interface boards may be needed to connect the detector and pumps and fraction collector e 3 wire LC interconnect cable R007200141 or 01 107441 00 for most connections e LC analog signal cable 01 107442 00 for the detector to the Analog and ground points on J3 on the Varian 440 LC I O Interface Board e The Varian 440 LC I O Interface Board part number 02 102338 00 e 325 335 Relay Interface Board part number 02 101875 00 or 02 101875 90 e 210 218 Rear Back Panel Interface part number 51 408927 00 supplied with the pumps Publication no 8510253500 Issue 1 August 2009 21 of 88 Varian 440 Fraction Collector Service Manual 210 Pump 03 93550001 or 218 Pump 03 936500 01 Digital inputs Manual Injection valve and cable Rheodyne 7725i RE7725i P C N wy STOP DGround Analog signals and relays Integrator High Analog jin egrator High Analog jn Rear back panel interface shipped with the 210 218 pump part nu
24. of field servicing it is Varian s policy to troubleshoot and repair to a modular level at the fraction collector location Software diagnostic routines are available to aid in fault isolation These are to be used in conjunction with conventional test equipment to effect on site repairs In situations where minimizing downtime is the prime consideration board or module exchange should be considered but only after preliminary attempts to repair have proven unsuccessful Component level repair should not be attempted outside a designated service repair center Electronic assemblies with surface mount components are not field repairable Spare Parts Policy Operational spare parts for all Varian instruments and major accessories will be held in stock for a period of five years after shipment of the final production unit Spare parts may be provided after this five year period but only on an as available basis Operational spare parts are defined as those individual electrical or mechanical parts that are susceptible to failure through fair wear and tear during their normal operation Examples include resistors inductors capacitors semi conductor devices relays lamps temperature probes detectors elements motors wiring board assemblies and the like General hardware for example sheet metal parts structural members assemblies and castings will normally outlast the useful life of the detector and therefore will only be suppli
25. supplied in Microsoft Windows MDS manufacturing amp diagnostics software Before starting 1 Remove the rear panel refer to Section 5 to access the BOOT button on each installed PCB and visual confirmation of the internal jumper settings 2 Connect the power cable and the serial communication cable to the fraction collector and turn the unit ON 3 Open up the SPS MDS and check the main screen Figure 29 for correct DIP and Jumper settings This also displays the current Main Board Firmware and Accessory Board Firmware You may be prompted to check your communications port settings Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual U MDS Manufacturing amp Diagnostics Software Com 1 9600 AIM3000 mode Varian ELO E E File Communication Port Yiew Tools Help General Details Address of Installed Accessory Boards Customer Varian GEBRBRBRBEBRRBERB Accessory Board Version Details Serial Number EL 07073734 Main Board Details Model SP39 Not Used Not Used Not Used Not Used Not Used Probe Monitor Relay AIM3300 Defaults Loaded at reset Config Checksum afSb3df1 AIM3200 Defaults Loaded at reset Program Checksum a502091d Test hode Swi Swi Relay Status B App DIP Status Jasi Moro Jumper Status System Firmware PIR Firmware Version 1 20 PR C2 07 Copyright C Ai Scientific 2007 System ID 0000105154c7 PNOA MDA Test Mode
26. the sample collection tube properly A value of about 150 is a good value to use Once you are happy with the setup click on the Start button and the probe will move through all the positions for the number of racks you have selected 7 7 3 Calibrating the 440 LC Fraction Collector The fraction collector should be calibrated when any of the following conditions occur e Loss of alignment and re installing the factory calibration coefficients does not solve the problem e After mechanical repairs to the drive assemblies e Replacement of the main control board To properly calibrate the fraction collector s probe position both a lower level and an upper level plane calibration must be preformed The Fraction Collector then extrapolates the positions for all the tubes in any rack from the 3D reference points acquired during the calibration Before proceeding with the fraction collector alignment procedure make sure that you have all the tools necessary A fraction collector alignment kit SPS3 Alignment kit can be ordered under part number 99 101216 00 This kit consists of the following items e Assy probe 0 8 mm This is an SPS3 probe but it is longer than the one on the 440 LC and is easier to use for alignment e Alignment tool see Figure 40 e SPS 3 Autosampler Resource CD Ignore the included videos 72 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 7 7 3 1 U MDS Manufa
27. the software 1 Select the Tools option from the pull down menu and click on Sample Figure 56 This will open the window in Figure 57 where you can choose which racks the fraction collector has and the rack size Rack 0 Rack 1 Rack 2 Reset Z 60 v iv Rack 3 Line Delay 750 Rack 4 ters Rack 5 Rack 6 Rack 7 T Time in sample 500 wash height Joo Optimised Move jo Mat Type Bekari v E Moving Rinse Station BE Fixed Wash a Completed 0 Pause Stop Close Figure 57 Fraction Collector Rack Configuration Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 2 The different rack positions are shown in Figure 58 Figure 58 Rack Positions on the Fraction Collector 3 Select a Z down Figure 57 value so that you can easily see if the probe is going into the sample collection tube properly usually pick a value of about 150 Once you are happy with the setup click on the Start button and the probe will move through all the positions for the number of racks you have selected 4 Ifthe probe goes into all the fraction collector tubes without any problems then close the Varian MDS software and reconnect the serial cable from the fraction collector to the back of the 900 Series back panel Publication no 8510253500 Issue 1 August 2009 83 of 88 Varian 440 Fraction Collector Service Manual This page is intentionally left blank 84 of 88 Pub
28. the unit on again If the unit initializes but the fraction collector is off light does not come on then the indicator is faulty Fuse has blown Replace the fuse See Section 5 9 Page 42 No mains power Check the mains supply Internal power supply Contact your Varian representative is faulty The CPU activity The indicator is faulty Turn off the fraction collector Wait 10 seconds before LEDS on the rear of turning the unit on again If the probe arm initializes but the fraction collector the light does not come on then the indicator is faulty are off Further confirmation of a failure can be confirmed by observing the status of the other LED indicators the communications and by resetting the fraction collector by powering it off and then on If it does not perform the initialization sequence by driving the probe arm there is a fault The main electronics Contact your Varian representative board is faulty The internal power Contact your Varian representative Supply is faulty Fraction collector is Probe arm or probe are Turn the power off to the fraction collector not initializing unable to move to the Check for mechanical obstructions Correct positions Turn the fraction collector on to allow it to attempt the reset procedure Watch where the fraction collector fails during the initialization procedure See Section 7 6 Page 66 Contact your Varian representative Error status LEDs are See Section 7 3 Page 64 sh
29. 1180 sys_stx sys_conf_chksum env_t 180 env_t180_A a 1800 sys_etx 45 45 sys_prog_chksum env L270 env_t270_A aip_a_baud sys_ver PIR Firmware Boundary Variables odom_t_steps odom_z_steps hip_a_echo sys_id 0000105154c move min move z min model sys_dilutor 1 move mak move 2 maz 160 000000 z move_y_min eny_x_fez Place the mouse pointer over the variable name for a description of the variable and what units are used Refer to the Programmers Manual before adjusting move_y_max env_ _ cz variables Green OEM Variables Red Advanced OEM Variables User Variables tm_time sys_led_s1_relay_a tm_up_height 0 000000 wash_down_ht 150 000000 Read Variables Load Variables tm_down_height 155 000000 mama 150 000000 sp_mode 2_error_mode Figure 33 Variables Page 10 Click the Load Variables button Figure 33 and then select the txt file that has the calibration coefficients that you want to load up Figure 34 It is a good idea to make the variables file name one that reflects the serial number of the Fraction Collector If you have multiple files then add a date stamp to your file name to ensure that you can distinguish them from each other Open TextFile Look in Desktop e amp ak E Fe omy Documents 5 1 My Computer My Recent my Network Places Documents ELOZO E EL07073734_100908 txt Desktop My Network File name EL07073734_100908 txt x L Places Files o
30. Assembly Loom 3 way Valve Block Diagram 68 72 85 85 86 87 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 1 General Information Information in this manual is for use by Varian trained Varian qualified or Varian approved FSRs Field Support Representatives This manual provides information to maintain and service the fraction collector The service manual may be viewed online Site preparation and operator training are described in the Varian modular liquid chromatograph pre installation instructions and the fraction collector Hardware manual e Varian Modular Liquid Chromatograph pre installation guide publication number 8510253100 e Varian 440 LC Fraction Collector Operation Manual publication number 8510252500 The software installation guidelines are described in the operator s manual and in section 3 of this manual 1 1 How to Use this Manual The safety practices and hazards section described below are provided to help the user operate the fraction collector safely Read these safety practices thoroughly before attempting to operate the fraction collector and always operate the fraction collector in accordance with these safety practices All information contained in this publication is relevant to the fraction collector 1 2 Finding Information There is a comprehensive Table of Contents in the front section of the manual detailing each chapter
31. August 2009 Varian 440 Fraction Collector Service Manual 7 Remove the sample racks from the Fraction Collector tray With the sample racks off the tray you should be able to see 4 small dimples Figure 44 in the white plastic rack holder Figure 45 Each of these dimples corresponds to a position A B C D A Figure 44 Example Dimple in Position A Figure 45 White Sample Rack Holder Tray with 4 Dimples 8 Move the probe to position A by selecting the Move to Point A Figure 46 Lower the probe by selecting the Move_z button Figure 46 and change the depth of the downward stroke the higher the number the further down it goes In order to move the probe in the X and Y direction use the buttons marked ve X and Y Figure 46 The step increment can be adjusted by changing the value in the Increment pull down menu Figure 46 Publication no 8510253500 Issue 1 August 2009 75 of 88 Varian 440 Fraction Collector Service Manual U Calibration Com 1 9600 AIM3000 mode Varian Lower Plane Calibration Initial move_t0_A 10 0 Clear x and Y Offsets Move To Point A Capture Point Move To Point B Capture Point B Calibrate Calibration Check Move To Point C Move To Point D Upper Plane Calibration Initial move t0 10 0 Clear x and Y Offsets Move To Point A Capture Point 4 Move To Point B Capture Point B Calibrate Calibration Check Move To Point C Move
32. August 2009 41 of 88 Varian 440 Fraction Collector Service Manual 3 Enter a probe depth and then click Test to move the probe down to that depth 4 Repeat until the probe sits just above the tube so that it can travel uninterrupted across the tubes 5 When all other settings are selected in the Console software click Upload to save the settings to the fraction collector 5 8 Replacing the Tubing Use this procedure to replace the tubing from the valve to the detector or the valve to the waste Warning Chemical Hazard Inhalation Hazard Fire Hazard Tubing may contain residual chemicals which may be harmful if you come in contact with them Some solvents may have flammable properties To avoid injury or damage to equipment consult the MSDS and always follow appropriate safety guidelines when using chemicals and solvents and always wear appropriate safety equipment and clothing To replace the tubing 1 Turn the flow off 2 Open the purge valve to release any residual pressure 3 Unscrew the fitting from the valve at port 1 or 2 See Figure 16 Page 38 4 Unscrew the fitting at the detector outlet or back pressure restrictor if applicable 5 Replace the tubing and or fittings as needed Tighten the nuts to finger tight Tubing 0 02 inch ID 1 16 inch OD Nut Short 1 16 Flangeless Tefzel Ferrule 1 16 Flangeless Natural Tefzel 6 Close the purge valve Turn on the flow and check for leak
33. Firmware Genetic Board C Intemal Diluter Internal Pump Query Variables Hi Address Firmware Genetic Board Internal Diluter Internal Pump Query Variables Extemal Diluter Figure 30 Varian MDS software Diagnostics window 5 Click on the Variables button in the top right of the Diagnostics window Save the variables to a text file for reference later Close the Variables window The diagnostics screen displays the current status of the fraction collector including accessories Each button has Tool Tip text that can be accessed by moving the mouse pointer over a button and waiting for the Tool Tip to appear The fraction collector can be queried and tested for faults using the tools in the diagnostics page Publication no 8510253500 Issue 1 August 2009 67 of 88 Varian 440 Fraction Collector Service Manual 7 7 2 Reload Fraction Collector Calibration Coefficients 6 Connect the Fraction Collector serial cable directly to the serial port of the PC 7 Once the serial cable is connected to the PC power up the fraction collector and allow it to go through it s start up routine Then launch the Varian SPS MDS software U MDS Manufacturing amp Diagnostics Software Com 1 9600 AIM3000 mode Varian ELO E b Address of Installed Accessory Boards ii BERR BEBB Accessory Board Version Details Serial Number EL 07073734 Main Board D Not Used Not Used Not Used Not Used Not Used S
34. LC System Configurator Create a new system Select this option to create a new system configuration Update an existing system z Select this option to modify an existing system configuration To continue click Next lt Previous Next gt Close 2 Depending on the hardware connections select PC configuration or MIB 850 configuration Varian Modular HPLC System Configurator 1 0 1 0 5 xj PC configuration Select this option when connecting devices to the PC C MIB 850 configuration Select this option when connecting devices to an MIB Modify MIB parameters Mode Static DHCP IP _ _ Gateway Cid Netmask CZ Update Reboot Search for MIB 850 Stop lt Previous Close 3 Before creating a system specify the details of the system Yarian Modular HPLC System Configurator 1 0 1 0 a Bj xj Enter a System name configure your instrument below then click Create System System name Modularlnt Flows CIM Module fisocraticPump SSS v Analog input Com port E RS 422UnitID 5 5 Model Prostar210 Detectors mmea I Varian ELSD Compot E E As 422UnmtiD fr 3 T Varian RI Compot EE Model ProStar210 z I Varian Prostar 326 P Ai M Varian ProStar 335 IPI we Find Fraction Collector M 440 LC Compot f4 Manual injector Create System lt Previous Next gt Close Publication no 8510253500 Issue 1 August 2009 27 of 88 Va
35. Page rT Open Log Send Script Sent stepper read Reply 916 891 0 Options File Loops DB Variables Firmware Acc Firmware Calibration r Main Board Settings Jumper Read Represents 8 Not Used High or On 7 Not Used em Represents amp Not Used Low or Off 5 Not Used 4 Not Used 3 Probe Monitor Relay 2 AIM3300 Defaults Loaded at reset 1 AIM3200 Defaults Loaded at reset Publication no 8510253500 DIP Read Test Mode Swi sw2 1 Test Mode 2 Test Mode 3 Reserved 4 Reserved 5 Baud Rate On 19 2k Off 9600 6 On 4IM1250 Off AIM3000 Mode 7 TAR Expiry Timer On 4min Off 30s 8 On TAR Off Fixed Wash Reservoir Issue 1 August 2009 Varian 440 Fraction Collector Service Manual U MDS Manufacturing amp Diagnostics Software Com 1 9600 AIM3000 mode Varian ELO File Communication Port view BESSI Help General Details Diagnostics Ctrl D nstalled Accessory Boards Customer variar Manufacture Ctrl M BEBRBBBRERBBBB SA AA Calibration Ctrl C oard Version Details eral Number ELO O73734 variables Ctrl y Main Board Detais Model SPS3 AccuracyArray Ctrl a ie 8 Not Used 3 Z Accuracy Ctrl Z Ri 7 Not Used System Firmware PIR Firmwar mee 6 Not Used Version vi 20 PR C Perpendicularity Ctrl P 5 Not Used i Scientific Odometers Ctrl 0 4 Not Used System ID 000010515 3 Probe Monitor Relay Options 2 AIM3300 Defaults Loade
36. SE 250V 5A 5 x 20mm LONG For safety reasons any other internal fuse or circuit breaker is not operator accessible and should be replaced only by Varian authorized personnel See figure 19 20 and 21 on page 43 External connections Mains inlet coupler 6 A 250 VAC IEC type Mains power cord connector 10 A 250 VAC Complies with AS3112 USA 10A 125 VAC Complies with NEMA 5 15P 6 A 250 VAC Complies with CEE7 sheet vii or NFC61 303 VA Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 3 Installation Procedure 3 1 Hardware Installation This section provides instructions for installing the 440 fraction collector Detailed operating procedures are provided in the Hardware Operation Manual and also included in the online help Warning For electrical safety always position the fraction collector so that it is easy to operate the mains power switch or to remove the power cord Where 2 6 4 5 z 7 7 8 9 Figure 1 Fraction collector components 1 IRN Theta axis Z axis slide Retaining ring Probe mounting block Knurled mount nut on the probe assembly Ferrule holding the metal probe Probe Rack Rack location mat Internal modules covers and panels must not be removed except by Varian trained qualified or authorized Customer Support Representatives unless otherwise specified Make sure to open the packaging and chec
37. Select the program Features you want installed Feature Description amp Varian Modular HPLC System Galaxie Driver Varian 441 ation Ganges Installshield Continue to install when prompted j Yarian Modular HPLC System Galaxie Driver InstallShield Wizard Ready to Install the Program The wizard is ready to begin installation Installshield Then finish The InstallShield Wizard has successfully installed the selected Features Click Finish to complete the installation TF you have installed the Varian Modular HPLC System Galaxie driver the Varian Modular HPLC System Setup Wizard will now run Finish Cancel The following sections provides instructions on how to configure your Varian 440 LC Fraction Collector software depending on the system options mentioned above Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 3 2 1 Configuring Fraction Collector in Galaxie Software In order to operate the Varian 440 LC using Galaxie software you must configure a system 1 Once the software installation is finished if the galaxie driver was part of the selections in step 4 of section 3 2 the Setup Wizard opens up Select either to create a new system or to update an existing system Varian Modular HPLC System Configurator 1 0 1 0 Afek ap a8 VARIAN Welcome to the Varian Modular HP
38. To Point D Set Point Set Point B Set Point C Set Point D Use number keys to move the Autosampler NumLock must be on Use the left pull down menu to change the magnitude of the movement in mm move t0 eny 90 eny ti80 eny t 270 0 0 0 0 Upper Plane l2m_xtrans_4 l2m_ytrans_4 move_rad_A mo WA eny t 90 A envy t 180A env 2704 bp fb b b bh p p _LowerPlane Reset_z Close Figure 46 Calibration Page 9 When the probe is correctly positioned over position A then press the Capture Point A button Fig 46 to lock in the coordinates 10 Repeat the above steps for Point B making sure that you use the Move to Point B and Capture Point B button Figure 46 11 Once both the A and B points have been calibrated click on the Calibrate button to lock in the calibration coordinates of the lower level plane Figure 47 12 The Calibration can be checked by moving the probe to position C Move to Point C Figure 47 The probe should be within 1 mm of the point C Moving the probe to position D Move to Point D will further confirm assuming that it is also within 1 mm that the whole lower level has been calibrated properly 76 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual U Calibration Com 1 9600 AIM3000 mode Varian Lower Plane Calibration Upper Plane Calibration Initial move_t0_A Initial move t0 10 0 10 0 Set Point Clear and Y Offsets Clear x and
39. Varian 440 Fraction Collector Service Manual Installation Category II Pollution Degree 2 Safety Class 1 EN 61010 1 a 2 lt Publication no 8510253500 Issue 1 VARIAN August 2009 Varian 440 Fraction Collector Service Manual Varian Inc Serving Industries Worldwide Biosciences Pharmaceuticals Clinical Research and Forensics Food and Agriculture Chemical Analysis Environmental Fuels and Energy Material Sciences Sales and Service Contact Details North America 800 926 3000 925 939 2400 Europe The Netherlands 31 118 67 1000 Asia Pacific Australia 613 9560 7133 Latin America Brazil 55 11 3238 0400 Varian Inc Web Site www varianinc com Varian Inc is the owner of copyright on this document and any associated software Under law the written permission of Varian Inc must be obtained before the documentation or the software is copied reproduced translated or converted to electronic or other machine readable form in whole or in part First published August 2009 This manual is the first version Varian and the Varian logo are trademarks or registered trademarks of Varian Inc in the U S and other countries 2009 Varian Inc Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Contents 1 General Information 1 1 Howto Use this Manual 1 2 Finding Information 1 3 Conventions 1 4 Tips 1 5 General 1 6 Design Features 1 7 Safet
40. action collector wiring diagram for non Galaxie systems showing the connections between the 325 335 detector and the 440 LC Fraction Collector I O Interface Board part number 02 102338 00 Publication no 8510253500 Issue 1 August 2009 23 of 88 Varian 440 Fraction Collector Service Manual J4 Fraction collector digital inputs from a detector typically or other relay signal input J2 Fraction collector relay outputs af Plug into the I O port on the side of the 440 LC See Figure 8 i gt hats IA CO J3 Add marks to the analog signal by connecting the detector and 210 218 pump Figure 12 440 LC Fraction Collector I O Interface board p n 0210233800 See the Varian Modular HPLC System Driver Installation and Configuration Instructions publication number 8510252400 for instructions on how to install the 440 LC Console software that you can use to control the fraction collector 3 2 Software Installation There are two possible ways to connect your Varian 440 LC Fraction Collector e With an existing or new modular system and Galaxie software e With a modular system with no software or software other than Galaxie controlling the HPLC You will need to install the Varian 440 LC software accordingly Install the software as followed 24 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 1 Insert the 440 LC driver disk and allow the installer to
41. are e With a Varian 900 LC Series HPLC system and Galaxie software e With a modular system with no software or software other than Galaxie controlling the HPLC Fraction collector control is through the Varian 440 LC Fraction Collector Console software provided on the driver installation disk 4 1 Turning on the Instrument and Software Before starting the Varian 440 LC Fraction Collector carefully read the Safety Practices and Hazards section at the front of this manual posing a potential hazard due to its moving parts Do not place your hands or objects near the probe arm when the 440 LC is turned on Warning Moving Parts The probe arm of the Varian 440 LC can move without warning To switch on the Varian 440 LC Fraction Collector 1 Ensure the Varian 440 LC is connected via a three pin earthed power cord to a three pin grounded power outlet 2 Turn on the power using the switch on the connection panel Figure 8 Page 19 which is located on the rear of the control column Note The Varian 440 LC will start up and perform an initialization sequence The initialization sequence moves the probe arm through the extent of the range of movement and finishes with the probe repositioned at the back left of the fraction collector Should the Varian 440 LC fail to initialize refer to Section 7 6 Page 66 3 Turn on the software a For Galaxie users click Start gt Programs gt Galaxie gt Galaxie b For non Ga
42. ate and the z axis carriage 2 Locate the z axis shafts into the retaining holes in the accessories plate and press until fully home 3 Holding the shafts in place check that the z axis carriage moves freely through its full travel along the z axis shafts 4 Install the probe arm covers Remove the power supply 1 Remove the rear panel 2 Disconnect the 6 way power cable connector and the 5 way ground cable connector from the power supply 3 Remove the three M4x8 screws and star washers that secure the power supply 4 Remove and discard the power supply Install the power supply 1 Align the lower screw mounting holes and insert and tighten two M4 x 8 mm screws with star washers 2 Insert one M4 x 8 mm screw with star washer into the power supply top mounting lug and tighten the screw 3 Connect the 6 way power cable connector and the 5 Way ground cable connector to the power supply 4 Install the rear panel Publication no 8510253500 Issue 1 August 2009 53 of 88 Varian 440 Fraction Collector Service Manual This page is intentionally left blank 54 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 6 Spare Parts For additional spare parts and consumables ordering information refer to the Varian Inc Web site www varianinc com 6 1 Consumables 6 1 1 Sample Racks Capacity no tubes x OD_ Part number 60 x 16 mm 6 1 2 Rack Overlay
43. bing You will need to fit the black tubing back over the Tefzel tubing and metal probe once the new tubing has been installed 14 Join the probe s Tefzel tubing to the HPLC system s detector outlet or back pressure restrictor if one is present on your system 15 Determine the volume delay time See Section 5 6 You will need to reset the volume delay in the Galaxie software or in the 440 LC Fraction Collector Console software Determining the Volume Delay he EN a Caution Warning Chemical Hazard Inhalation Hazard Fire Hazard Tubing may contain residual chemicals which may be harmful if you come in contact with them Some solvents may have flammable properties To avoid injury or damage to equipment consult the MSDS and always follow appropriate safety guidelines when using chemicals and solvents and always wear appropriate safety equipment and clothing To determine the correct volume delay You will need a syringe filled with air and a weighed glass cylinder 1 Turn on the Galaxie or the Console software your HPLC system and the Varian 440 LC Fraction Collector 2 Select the appropriate system in the Galaxie software or the appropriate COM port and then click Connect in the Console software 3 Turn the divert valve to allow liquid to flow through the probe Caution If the divert valve is in the collect position liquid will flow out of the probe Place a container underneath
44. carriage block with the wires facing the column 3 Secure the theta axis stepper motor to the x axis carriage block using the four M3x6 screws Take care not to damage the theta axis driven gear 4 Slide the spacer onto the stepper motor shaft Publication no 8510253500 Issue 1 August 2009 51 of 88 Varian 440 Fraction Collector Service Manual 9 Rotate the drive gear to align the grub screw to the flat of the stepper motor shaft Push the drive gear fully home and tighten the grub screw Slide the encoder wheel onto the stepper motor shaft Rotate the encoder wheel to align the grub screw to the flat of the stepper motor shaft and tighten Install the theta axis PCB 10 Install the x axis cover 5 20 Remove and install the z axis stepper motor 5 20 1 Remove the z axis stepper motor 1 2 3 6 5 20 2 Install the 1 gt Remove the probe arm covers Remove the z axis timing belt Loosen the grub screw securing the encoder wheel and carefully remove the encoder wheel Remove the two M3x12 screws and M3 star washers securing the stepper motor to the z axis centre plate Remove the two M3x6 screw and star washers that secure the stepper motor and centre plate to the theta axis shaft Remove the stepper motor and discard z axis stepper motor Secure the stepper motor and centre plate to the theta axis shaft using the two M3x12 screws and star washers Secure the stepper motor to the z axi
45. ck SP53 0910188900 fover probe arm fromt SPS3 910189100 a foverxaxsendcap 2970189200 6 over back panel SP53 pororo5600 Base support tube b71ooa7ao0 o Baseleg Woon S e OGIO Platen 440 LC plastic spil ray 0910281300 S S 110220 ___Berew a xemm isto250500 _ Berew 3x 12mm 2510250700 C orewmex30cap 51025090 _ Serew Wa xtomm feran MWasher stara 722510251500 Washer star MA ji510251600 Screwmdribmm Jisi0254700 0 SerewM4x8mm 7151025480 Assy Main Cover 440 LC 0110824500 DrainPipe 0110827500 56 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 6 3 Control Electronics 11 14 ID Description Partnumber H Assy stepper motor E Or 0110726290 2 Assy cable theta axis FFC 3 Assy cable z axis and x axis FFC 4 AssyloomLED 0110728300 5 Assy loom AC earih_ 110726400 Assy loom ground wire 7 Assey loom DC power 7010728600 8 JAssyioomACpowr 0110728700 Assy cable DB9F to DB9M extension 7910046300 Assy cable aux com control 0110729100 _ Assy loom 3 way valve includes solenoid 0110824700 20a FuseSA CS 910009100 O e0b FueeS5A CS 910009100 Publication no 8510253500 Issue 1 August 2009 57 of 88 Varian 440 Fraction Collector Service Manual 6 4 Probe Arm LILI 21 19 30a 30b 27 29 18 12 24 26 12 27 28 18 12 5 14 31a 31b 10 Cable clamp z axis ircli
46. ck 1 tube 1 000000000000 3 Front of the fraction collector OO00 00000000 900000000000 0900000000000 0900000000000 0900000000000 000000000000 0900000000000 0900000000000 000000000000 000000000000 900000000000 3 Figure 7 Rack location mat 3 1 1 6 Connecting the Power Before connecting any part of the Varian 440 LC Fraction Collector to the mains power supply check you have the correct power supply Warning Electrical Shock Fire Hazard Application of the wrong supply voltage can create a fire hazard or a potentially serious shock hazard and can seriously damage the instrument and any attached ancillary equipment Do not connect the instrument to the mains power until you have ensured that the mains power supply from the outlets in your laboratory is within the range The connection panel of the Varian 440 LC is located on the rear of the control column The panel contains relays indicators DIP switches the power socket and switch and a RS 232 port for communication between the Varian 440 LC and the computer that is running instrument control software 18 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 1234 fo se ee Error indicators fsa ___ Reset button ig DIP switches DIP A I O port Relay status indicators saua Status indicators o Cc g Auxiliary communication port 6 wre e Instrument communicat
47. cord adheres to the color coding and polarity described in the manual and all local building safety codes e Good grounding earthing is essential to avoid a potentially serious electric shock hazard Ensure that there is an integral ground connection between the metal base of the detector and the 3 pin earth grounded receptacle Consult the manuals or product labels supplied with your PC for the relevant grounding requirements e Replace blown fuses with fuses of the size and rating shown on the fuse panel or in the manual e Replace faulty or frayed power cords immediately with the same type and rating e Make sure that voltage sources and line voltage match the value for which the instrument is wired e Avoid using power supplies from a source that may be subject to electrical or RF interface from other services for example large electrical motors elevators and welders The fraction collector electronics are powered by the personal computer PC controlling the instrument The fraction collector is powered by mains power 110V to 240V and will contain dangerous voltages when in operation Do not remove any of the fraction collector panels or covers if the fraction collector is in use and do not operate the fraction collector with the panels or covers removed The fraction collector is certified to European safety requirements per Council Directives EN 61010 1998 Safety Requirements for electrical equipment for measurement control and
48. cturing amp Diagnostics Software Com 1 9600 AIM3000 mode Varian ELO Figure 40 Top Layer Plane Alignment Tool Fraction Collector Calibration Lower Level Plane 1 Attach the Calibration Probe Assy probe 0 8mm to the Fraction Collector 2 Connect the Fraction Collector directly to the PC with the Varian MDS Software via a serial cable 3 Switch on the Fraction Collector 4 Launch the Varian MDS program and select the Diagnostics button SEE File Communication Port Yiew Tools Help General Details Customer Serial Number m Address of Installed Accessory Boards exe Board Version Details Marian System Firmware Version System ID Config Checksum Program Checksum Relay Status 1 LED Status Digital Status SPS3 PIR Firmware 1 20 PR C2 07 Copyright C Ai Scientific 2007 0000105154c7 af3b3dh a502091d 0000000900 1 8 Not Used Not Used Not Used Not Used Not Used Probe Monitor Relay AIM3300 Defaults Loaded at reset 4IM3200 Defaults Loaded at reset eee Test 1 Jumper Status Test Mode Test Mode Test 2 Reserved Test 3 Reserved Baud Rate ON 19 2K OFF 9600 ON AIM1 250 OFF AIM3000 Mode MRS Expiry Timer ON 4min OFF 30s ON MRS OFF Fixed Wash DIP Status Represents High or ON Represents Low or OFF Refresh OYMnHhRWON gt Manufactu Sent aux_read Publication no
49. d at reset Config Checksum afSb3df1 1 AIM3200 Defaults Loaded at reset Program Checksum a502091d Test Mode Suvi Swv2 Relay Status 4 E B ID DIP Status T Wa i a S1 LED Stat Reserved Tet aa si 4 Reserved testy Digital Status l 200000 I 0 Represents High or ON 3 Paa Na TIAI Orr eoo l Represents Low or OFF 7 MRS Expiry Timer ON 4min OFF 30s 8 ON MRS OFF Fixed Wash Refresh Manufacture Diagnostics Exit Sent stepper read l Received OK 916 891 011 Port 1 Open 11 09 2008 10 14AM Figure 37 Main Page of the software 13 Select the Tools option from the pull down menu and click on Sample Figure 37 This will open the window in Figure 38 where you can choose which racks the Fraction Collector has and the rack size U Sample Com 1 9600 AIM3000 mode Vari Rack 0 Rack 1 Rack 2 Racka Line Delay 50 Rack 4 Po Rack 5 Rack 6 Rack 7 77 Time in sample 500 wash height 100 Optimised Move fo Mat Type Bel Art v Random B Moving Rinse Station Reset Septum Piercing Fixed Wash E SetT imd Pause Stop Close Completed 0 Figure 38 Fraction Collector Rack Configuration 14 The different rack positions are shown in Figure 39 Publication no 8510253500 Issue 1 August 2009 71 of 88 Varian 440 Fraction Collector Service Manual Figure 39 Rack Positions on the Fraction Collector 15 Select a Z down Figure 38 value so that you can easily see if the probe is going into
50. d in the Pre installation manual e 8510253100 Varian Liquid Chromatography Modules Pre Installation Manual Note A copy of the checklist must be received from the customer before attempting to install the fraction collector Note A copy of the above mentioned manuals can be obtained from Varian or downloaded from the Varian support website 2 1 Weights and dimensions Ax AN Warning Some of the packages are large and heavy To avoid the chance of injury to personnel or accidental damage to the equipment always use two or more people when handling the packages or lifting equipment into position Never attempt to lift the packages alone System Unit Height Width Depth Weight Fraction Collectors 440 LC fraction collector 480 mm 400 mm 600 mm _ 18kg unpacked 19 in 16 in 24 in 34 Ib Shipping dimensions 500 mm 760 mm 760mm 31kg 20 in 30 in 30 in 69 Ib 2 2 2 2 1 Mains supply Electrical specifications The installation of electrical power supplies must comply with the rules and or regulations imposed by local authorities responsible for the supply of electrical energy to the workplace Publication no 8510253500 Issue 1 August 2009 11 of 88 Varian 440 Fraction Collector Service Manual 2 2 2 2 2 3 12 of 88 Fuses Note Warning Good electrical grounding is essential to avoid potentially serious shock hazards A 3 wire outlet with ground connection must b
51. e provided for the detector Make certain that power outlets are earth grounded at the grounding pin All power supplies must be single phase AC voltage three wire system active neutral earth and should be terminated at an appropriate power outlet receptacle that is within reach of the fraction collector power cord assembly For safety reasons a separate power outlet receptacle should be provided for each unit in the system Do not plan to use extension cords or outlet adaptors All 440 fraction collectors are supplied with a 2 meter 6 6 long power cord and three pin plug assembly that is compatible with common standards applicable in the local area Avoid using power supplies from a source that may be subject to electrical or RF interference from other services large electric motors elevators and welders for example Required voltage supply 100 240 VAC 10 VAC 50 60 1 Hz 1 8A 75 Watt When replacing fuses ensure the power to the unit has been turned off and the power supply cable has been disconnected Use a thin blade screw driver to unclip the cover and slide out the pull out fuse compartment Remove the blown fuse and replace with a fuse of the same type and rating Slide the pull out fuse compartment back into the unit and clip the cover back into place Reconnect the power supply cable and switch on power to the unit Part number Description 19 100107 00 FUSE 250V 3 15A 5 x 20mm LONG 19 100091 00 FU
52. e no user adjustments necessary under normal operating conditions This chapter includes the Varian 440 LC Fraction Collector maintenance requirements that may be carried out by a Field Service Representative FSR 5 2 Routine Maintenance While there are no parts that require frequent maintenance you should always e Confirm that the tubing from the probe to the HPLC system is secure and kink free e Check the probe and tubing for blockages e Clean any spills by removing draining and rinsing the spill tray 5 2 1 General Cleaning Cleaning should be performed using warm water and a soft lint free cloth This will help preserve the plastic components and painted surfaces Any spills should be wiped up immediately To avoid clogging the probe and tubing a rinse solution should always be pumped through the fraction collector at the end of a run If necessary the fraction collector probe can be removed from the fraction collector and placed in an ultrasonic bath for cleaning e The exterior surfaces of the fraction collector should be kept clean and any spills should be wiped up immediately e All cleaning should be done with a soft lint free cloth If necessary this cloth can be dampened with warm water or a mild detergent Do not use organic solvents or abrasive cleaning agents This will assist to preserve the plastic components and painted surfaces e Check the x axis and z axis timing belts for cracks splits or c
53. e x axis stepper motor and over the tensioning idler pulley Using the x axis tensioning idler allow the spring to apply tension to the timing belt Re tighten the screw Slide the x axis carriage along the x axis shafts several times to ensure smooth movement Slide the encoder wheel onto the stepper motor shaft Rotate the encoder wheel to align the grub screw to the flat of the stepper motor shaft and tighten Install the x axis PCB Install the x axis cover Install the rear cover 5 19 Remove and install the theta axis stepper motor 5 19 1 Remove the theta axis stepper motor 1 Remove the x axis cover 2 Remove the theta axis PCB 3 Loosen the grub screw on the theta axis encoder wheel and slide the encoder wheel off the motor shaft 4 Loosen the grub screw from the stepper motor drive gear and slide the gear off the motor shaft 5 Slide the spacer off the motor shaft 6 Rotate the theta axis driven gear so that it is possible to remove the four M3x6 screws that hold the theta axis stepper motor to the x axis carriage block 7 Remove the four M3x6 screws that hold the theta axis stepper motor to the x axis carriage block Take care not to damage the theta axis driven gear 8 Carefully remove the theta axis stepper motor 5 19 2 Install the theta axis stepper motor 1 Carefully feed the theta axis stepper motor cable into the x axis carriage block 2 Orientate the theta axis stepper motor into the x axis
54. ector Use 3 wire LC interconnect cables VO Interface R007200141 or 01 107441 00 Figure 10 Fraction collector example wiring diagram for non Galaxie systems showing the 440 LC Fraction Collector I O Interface Board and 210 218 pumps to 325 335 detector connections 22 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 325 Detector 335 Detector aa gt 02 101871 00 02 101946 00 02 101872 00 02 101948 00 02 101873 00 02 101874 00 LC analog signal cable 01 107442 00 To J3 on the 440 LC Analog 2J9 J14 Analog signal Out Peak sense relay to J4 on the 440 LC Advance gt Relay Peak Sense 1 Only Time slice and Pulse available 7 with the 325 detector 2 Pulse for peak start valley and peak end available on the 335 Peak sense relay to J4 on the 440 LC Advance To J4 on the 440 LC Start Relay 2 Program to drive the fraction collector Start input in a detector method To J4 on the 440 LC Start To J4 on the 440 LC Valve Relay 1 Program to drive the fraction collector Divert Collect valve input in a detector method DODADADDOAONDO Note for Relay 1 4 You must activate and Relay Out de activate each relay on separate lines in a method pe d To J4 on the 440 LC Valve 02 101875 00 or 02 101875 90 325 335 Relay Interface Figure 11 Fr
55. ed on an availability basis after the final production unit is manufactured Proprietary items and components that are unique to the Varian equipment should be sourced from the Varian Service Center Other components may be sourced locally unless otherwise specified in this manual Warranty All Varian products are warranted against defects in materials and workmanship but not against consequential damages Full details relating to the terms of warranty are available from any Varian Sales and Service Office or Agency No other warranty is expressed or implied Consumable items such as but not limited to light sources temperature probes and other glassware items are subject to a limited or pro rated warranty The Varian warranty provides for specific legal rights There may also be other rights that vary from country to country or region to region The warranty applies only to the installed Varian equipment and cannot be extended to cover the associated miscellaneous installations that remain the customer s responsibility By the same reason the warranty does not cover problems that may be caused by misuse of the equipment wrong applications operator s inexperience etc Standard Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Warranty will cover 12 months though this may vary according to location 1 8 5 OEM Warranty Personal Computers PCs printers modem other PC accessor
56. ent Always ensure that laboratory safety practices governing the use handling and disposal of hazardous materials are strictly observed These safety practices should include wearing appropriate safety clothing and safety glasses Consult the manuals supplied with your PC monitor and for their specific ventilation requirements Great care should be taken when working with glass or quartz parts to prevent breakage and cuts This is especially important when removing or replacing glass fraction tubes Use only Varian supplied spares with your instrument Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 1 7 7 Information Symbols The following symbols appear on the fraction collector to provide you with additional information l Mains power on 0 Mains power off Fuse AI Single phase alternating current When attached to the rear of the instrument indicates that the product complies with the requirements of one or more EU directives When attached to the rear of the product SP indicates that the product has been certified evaluated to CSA 1010 1 and UL 3101 1 When attached to the rear of the product indicates that the product complies with the Australian EMC requirement 1 7 8 Warnings and Cautions A Warning message is used in the text when failure to observe instructions or precautions could result in death or injury The following is a list of symbols tha
57. epper motor 1 2 3 4 on 10 Remove the rear cover Remove the x axis cover Remove the x axis PCB Loosen the grub screw on the Encoder wheel and remove the encoder wheel Loosen the x axis belt tension by using the x axis tensioning idler Remove the belt from the x axis idler pulley and x axis drive pulley Loosen the grub screw on the drive pulley and remove the pulley Remove the spacer Loosen the two M4x12 screws and carefully remove the x axis motor bracket and stepper motor Remove the four M3x6 screws and star washers from the x axis motor bracket and discard the x axis stepper motor Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 5 18 2 Install the x axis stepper motor 1 10 11 12 13 14 Secure the x axis motor bracket to the stepper motor with the four M3x6 mm screws ensuring that the PCB mounting holes are orientated to the same side of the stepper motor as the wiring connections Slide the spacer down the stepper motor shaft Slide drive pulley down the stepper motor shaft until firmly positioned against spacer Rotate the drive pulley on the stepper motor shaft to align the grub screw to the flat of the stepper motor shaft and tighten Slide the x axis motor bracket into the slot in the chassis column Tighten the two M4x12 screws to retain the x axis motor bracket in position Fit the timing belt over the drive pulley on th
58. f type Text Files TXT Cancel Figure 34 Calibration File Selection Publication no 8510253500 Issue 1 August 2009 69 of 88 Varian 440 Fraction Collector Service Manual 11 Once you have selected the desired file to upload and pressed the Open Figure 34 You will then need to select the Write All Figure 35 option to write the variable to the firmware You can then close the Variables page U Variables Com 1 9600 AIM3000 mode Varian ELO7073734 Motion Variables Jo 200000 gB 000000 1 250000 Calibration Variables stpt_x_vi f 21 013924 move rad A 12m trans A stpr_zd_vi move_rad 120 925003 2 190000 stpr_x_vf stpr_zd_vf 2m_xtrans ipi ka stpr_zd_a l2m_ptrans 2m_ptrans_A l2m_theta stpr_x_i stpr_zd_j 2m_theta_A stpr_t_vi stpr_zu_vi move t0 move DA stpr_t_vt stpr_zu_vf move_pi move pi A stprta 290 000000 290 000000 0 145160 25 000000 move_xmo_A esezso0 move_mpsx_A 0086275 mama p env t 90A 1800 eny L270A fisce 0 000000 160 000000 env_x_fez 1000 env_x_ cz 2400 tm time fo sys led si relay a JO tm up height fo o00000 wash down ht 150 000000 tm down height 155 000000 rack down ht 150 000000 2_eror_mode 0 stpr_zu_a move tr move HA stpr_ 0 000150 stpr_zu_j move_tps move_tps_4 7 move mo 25 000000 System Variables stpr_x_enror_band 8 odom_x_steps moyo ampa 134 move mpsz 120 af9b3df1 a502091d stpr Leror band odom t steps
59. following symbol may be used on warning labels attached to the instrument When you see this symbol you must refer to the relevant operation or service manual for the correct procedure referred to by that warning label 1 7 9 Color Coding The various indicator lights appearing on Varian instruments and associated accessories are color coded to represent the status of the instrument or accessory e A green light indicates the instrument is in normal standby mode e Anorange light indicates that a potential hazard is present e A blue light indicates that operator intervention is required e Ared light warns of danger or an emergency 1 7 10 Federal Communications Commission FCC The following is a Federal Communications Commission advisory This equipment generates uses and can radiate radio frequency energy and if not installed and operated in accordance with the instruction manual may cause interference to radio communications It has been tested and found to comply with the limits for a Class A computing device 6 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual pursuant to Subpart Jof Part 15 of FCC Rules which are designed to provide reasonable protection against such interference when operated in a commercial environment Operation of this equipment in a residential area may cause interference in which case the user at their own expense will be required to take whatever
60. he securing screws rotating the PCB clockwise whilst sliding out will help in removal 5 14 2 Install the theta axis PCB 1 Align the slots in the theta axis encoder PCB with the four M4x10 self tapping screws in the top of the x axis carriage assembly Publication no 8510253500 Issue 1 August 2009 47 of 88 Varian 440 Fraction Collector Service Manual 2 Ensure that the encoder wheel has located inside the sensor pick up area and carefully slide the theta axis Encoder PCB fully home against the screws and tighten all four screws 3 Install and check the encoder wheel alignment 4 Connect the theta axis motor cable to the theta axis PCB 5 Install the x axis cover 5 15 Remove and install the z axis encoder PCB 5 15 1 Remove the z axis encoder PCB 1 Remove the probe arm front cover 2 Remove the z axis FFC cable clamp and disconnect the FFC cable Take care not to damage the FFC cable 3 Disconnect the z axis motor cable 4 Remove the two MS8x6 screws that secure the z axis PCB to the captive nuts on the z axis centre plate 5 Carefully remove the z axis PCB 5 15 2 Install the z axis encoder PCB 1 Locate the z axis encoder PCB on the centre plate ensure the encoder wheel can turn freely through the encoder sensor mounted to the PCB 2 Secure the z axis encoder PCB to the captive nuts on the z axis centre plate using the two M3x6 screws Locate the side of the sensor with the white diode symbo
61. ies and OEM accessories used with the fraction collector are not covered by Varian warranty Generally this type of equipment is sourced locally and therefore local supplier or vendor warrantees will apply 1 8 6 Contract Maintenance After expiry of the warranty time the service and or repair of the fraction collector are handled on a time and materials basis in accordance with the Varian standard service or contract maintenance arrangements Quotations for contract maintenance may be obtained from any Varian Sales Office or Service Center Publication no 8510253500 Issue 1 August 2009 9 of 88 Varian 440 Fraction Collector Service Manual This page is intentionally left blank 10 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 2 Pre installation The Varian 440 fraction collector has been fully tested and proven to specification before dispatch from the manufacturing plant This chapter contains general information that is relevant to the preparation of an installation site and details the facilities which must be provided to ensure that the system can be effective Detailed operating procedures are provided in the operation manual or Help that is supplied with the instrument e 8510252500 Varian 440 LC Fraction Collector Operation Manual For installations that are to be done by Varian personnel the customer should complete the Pre installation checklist containe
62. ing 33 5 2 2 Cleaning the Probe 33 5 2 3 Cleaning the Spill Tray and Rack Mat 34 5 2 4 Lubrication 34 5 3 Component Installation and Replacement Procedures 35 5 4 Installing the Low Delay Volume Tubing 36 5 5 Replacing the Probe and Probe Tubing 37 5 6 Determining the Volume Delay 40 5 7 Determining the Correct Probe Depth 41 5 8 Replacing the Tubing 42 5 9 Replacing the Fuse 42 5 10 Removal and installation of covers 44 5 10 1 Rear panel 44 5 10 2 Probe arm assembly covers 44 5 10 3 X axis cover 44 5 11 Removal and installation of timing belts 45 5 11 1 Z axis timing belt 45 5 11 2 X axis timing belt 45 5 12 Removal and installation of PCBs 46 5 12 1 FFC cable connectors 46 5 13 Remove and install the x axis encoder PCB 47 5 13 1 Remove the x axis encoder PCB 47 5 13 2 Install the x axis encoder PCB 47 5 14 Remove and install the theta axis PCB 47 5 14 1 Remove the theta axis PCB 47 5 14 2 Install the theta axis PCB 47 5 15 Remove and install the z axis encoder PCB 48 5 15 1 Remove the z axis encoder PCB 48 5 15 2 Install the z axis encoder PCB 48 5 16 Remove and install the main PCB 48 iv Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 5 16 1 Prepare the main PCB for installation 48 5 16 2 Remove the main PCB 49 5 16 3 Install the main PCB 49 5 17 Remove and install the accessories PCB 50 5 17 1 Remove the accessories PCB 50 5 17 2 Install the accessories PCB 50 5 18 Remove and ins
63. ing may contain residual chemicals which may be harmful if you come in contact with them Some solvents may have flammable properties To avoid injury or damage to equipment consult the MSDS and always follow appropriate safety guidelines when using chemicals and solvents and always wear appropriate safety equipment and clothing To fit the low delay volume tubing 1 Identify and remove the tube that runs from the divert valve to the fraction collector probe Port 3 on the divert valve Where 1 To waste 2 From detector outlet or back pressure restrictor 3 To the probe Figure 14 Divert valve tubing positions 36 of 88 2 Cut the supplied narrow bore tube 0 10 inch ID or 0 025 cm tubing to an equal length 3 Use the original nut and the new ferrule supplied with the tubing kit 4 Screw the tubing into the divert valve and insert it into the fraction collector probe 5 Repeat this process for the tubing between the detector or back pressure restrictor and the divert valve using the original nut and the new ferrule supplied with the tubing kit 6 Follow the next procedure Replacing the Probe and Probe Tubing to remove the existing tubing and replace it with the low delay volume tubing into the metal probe Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 5 5 Replacing the Probe and Probe Tubing Where 1 Z axis slide 2 Top knurled mount nut on
64. ion port Power supply unit Not supported Figure 8 The connection panel of the Varian 440 LC Before connecting the mains power for the first time check each fuse as described in Section 5 9 Page 42 Electrical power for the Varian 440 LC must be provided through a three wire outlet with ground connection The outlet must be rated for at least 75 VA Warning Electrical Shock Good grounding is essential to avoid a potentially serious shock hazard Ensure that power receptacles are earth grounded at the grounding pin Three power cables are supplied with the Varian 440 LC Select the correct one for your location Ensure that the power switch is turned off 0 and then plug one end of the power cable into the Varian 440 LC both the switch and the power socket are located on the connection panel at the rear of the control column and the other into the mains power outlet Note In some countries it may be necessary to fit a suitable three pin power plug to the cord A three pin earthed power outlet must be used Ensure the Varian 440 LC is always connected to the mains supply protective earth Do not switch on the Varian 440 LC until all checks and settings for the Operating Mode have been completed When the Varian 440 LC is turned on it will go through initialization tests and set the probe position If the initialization process is not successful refer to the troubleshooting information that is provided w
65. is important to ensure that the length of the tubing from the probe to the valve allows the probe arm to move freely in all axes If it is too tight it will restrict movement and may cause movement failure If it is too loose the probe arm may become tangled in the tubing as it moves Tubing length for the tubing between the valve and the detector depends on fraction collector placement If the tubing is too long between the detector output and the fraction collector probe end your samples may not correspond correctly to the fraction collector markers on your chromatogram When choosing the length of tubing ensure it is long enough to allow free movement of the arm but not too long to delay sample delivery into the tubes The delay volume calculation will compensate for this effect 13 Move the probe arm to the right side of the fraction collector and rotate the probe arm fully to the right to check that the tubing is completely free to move 14 Manually position the probe arm in the middle along the X axis Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 15 16 17 18 Rotate the probe arm through its full extent to check that the tubing is free to allow full movement in all directions If the length is not correct remove and then replace the tubing install the fittings and tubing onto the fraction collector Repeat steps 12 14 to test the tubing length Adjust the length
66. ithin Section 7 Ensure that the probe arm s movement is not interfered with during operation initialization During the initialization sequence 1 The probe rises to the full extreme of the Z axis Publication no 8510253500 Issue 1 August 2009 19 of 88 Varian 440 Fraction Collector Service Manual 2 The probe travels to the full extremes of the X axis and rotates to the full extremes of the Theta axis 3 Then the probe is positioned at the back left of the fraction collector 3 1 1 7 Instrument Communication Port The instrument communication port see Figure 8 is used to connect the Varian 440 LC to the computer or MIB controlling the instrument 3 1 1 8 VO Port The I O port is used with a 440 LC Fraction Collector I O Interface board part number 0210233800 when connecting the fraction collector to a non Galaxie HPLC system 3 1 1 9 Auxiliary Communications Port The Auxiliary communications port provided on the Varian 440 LC is not used in current Varian software 3 1 2 Connecting Fraction Collector to LC System There are two possible ways to connect your Varian 440 LC Fraction Collector e With an existing or new modular system and Galaxie software See Section 3 1 2 1 Page 20 e With a modular system with no software or software other than Galaxie controlling the HPLC See Section 3 1 2 4 Page 21 The following sections provide instructions on how to connect your Varian 440 LC Fraction Collector to the syste
67. its power supply is operational Note The flashing only indicates that a character has been received It does not indicate that the command is correct If the fraction collector shows offline in the Status Overview or won t connect to the Console software follow these steps to troubleshoot 1 Check that the RS232 cable between the HPLC system or computer and fraction collector is firmly connected and screwed into place 2 Initialize the fraction collector to ensure that it is in a working state Turn off the power switch wait 10 seconds and turn the power switch on 3 When the software is started the software lights the LED linked to the RS232 connector 4 Check that the fraction collector LED flashes while commands are being sent to the fraction collector If there are flashes this indicates that the fraction collector has received a message from the instrument computer It will always respond on receipt of the Enter key regardless of the success of the message this will indicate the Baud Rate is correct 5 lf there are no flashes confirm that the fraction collector is functioning by watching for the CPU Activity LED to flash Refer to the image above for the location of this LED This should take about 10 15 seconds If this does not occur reset the fraction collector to ensure that it follows through the reset sequence and then wait until the CPU Activity LED flashes If this is successful then retest from step 4 above
68. k received goods against the packing list together with the customer Report any missing or incorrect items to the customer support organization of where the order was placed and have them send replacements if necessary Publication no 8510253500 Issue 1 August 2009 13 of 88 Varian 440 Fraction Collector Service Manual There are several steps involved in installing your fraction collector 1 Assembling the fraction collector 2 Connecting the fraction collector to the HPLC system 3 Determining the delay volume 3 1 1 Assembling the Varian 440 LC Fraction Collector Carefully follow the instructions provided below to assemble your instrument Assembling the 440 LC Fraction Collector includes installing the e Spill Tray e Rack Location Mat e Probe and tubing e Sample racks Note Position the fraction collector on the side of the HPLC system closest to the detector output to help decrease the delay volume 3 1 1 1 Installing the Spill Tray Where 1 Alignment pin underneath the raised locating tabs 2 Right side towards the back of the instrument Figure 2 Spill tray alignment pin and rack location mat raised locating tabs The spill tray will catch most solvent spills Always clean up spills immediately To install the spill tray 1 Ensure the alignment pins protrude above the base support tube 2 Locate the tray so that the two alignment pins at the rear of the base support tube fit into the slot
69. l Install and check encoder wheel alignment Connect the z axis FFC and motor cable Install the probe arm front cover ook w 5 16 Remove and install the main PCB 5 16 1 Prepare the main PCB for installation Light pipes Connector fasteners Connector shrouds Figure 25 Preparing the main PCB for installation 1 Ensure that the three light pipes on the 440 fraction collector have been fitted to the PCB 48 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 2 Take note of the orientation of the connector shrouds as they are free to fall off the connectors when the connector fasteners are removed 3 Remove the six connector fasteners from the connector shrouds and retain 5 16 2 Remove the main PCB 1 Remove the rear panel 2 Disconnect accessories cable if fitted 3 Disconnect the power cable 4 Disconnect the x axis and theta axis FFC 5 Remove the six connector fasteners 6 Remove the three M3 x 12mm screws and M3 star washers that secure the main PCB 7 Carefully remove the main PCB and discard 5 16 3 Install the main PCB M3x12 star washer P E P006 Figure 26 Install the main PCB 1 Position the main PCB with the light pipes and connectors correctly located in the cut outs in the side of the column case 2 Move the main PCB as required aligning the three mounting holes in the main PCB with the PCB spacers Publication
70. laxie systems double click the Varian 440 LC Console icon on the desktop 4 2 Creating a Method Using Galaxie Software When using the Varian 440 LC with an existing or new modular system and Galaxie software you will find step by step instructions for common operations in the Varian Modular HPLC System Help To access this information click Start gt Programs gt Galaxie gt Help gt Varian Modular HPLC System Help When using the 440 LC with a 900 LC Series HPLC you will find step by step instructions for common operations in the Varian 900 LC Series Publication no 8510253500 Issue 1 August 2009 31 of 88 Varian 440 Fraction Collector Service Manual HPLC Help To access this information click Start gt Programs gt Galaxie gt Help gt Varian 900 LC Series HPLC Help 4 3 Controlling Fraction Collector with 440 LC Console The Varian 440 LC Console is used to set up and monitor the fraction collector when Galaxie is not used as the HPLC control software To setup and monitor the fraction collector 1 Double click the Varian 440 LC Console icon to open the control software 2 Select the correct COM port for your fraction collector communication from the RS232 Com Port drop down menu 3 Click Connect Select or enter all required parameters For information on each setting hover your cursor over the field or information bubble to view the associated tip Click Upload to save the settings Click the Monitor
71. lication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 8 Electronic and mechanical schematics 8 1 Fraction Collector inter wiring Publication no 8510253500 Issue 1 August 2009 85 of 88 Varian 440 Fraction Collector Service Manual 8 2 Fraction Collector Power board Assembly Schematics GuvOd NIVW 3LLINOYId COCKE RECT PAS pa SRP EY SL Per Saal OF aa 1 PY 9 is NEZ dia BLX Jl OdN du Sl sousa yi Poyreds ssann S090 MGZL O WG Sorssas iy Poydeds ssaun SOHO AST HOI Sde SLU jy 0N c moneen sin san en yaan 3222222222 VJ RAZARAZEZA 224232 E kk RR REEE H mwao Maroro Cosota nt ii HH HE a pee a J E Fie ES mon asl ane hirer a ao a Pum amp WA peusagu ei 4 a oO gt oe amp gt a a an a I S Tas maza am ys KWAYA maT Pon Ep Tuge sep Le ask Al a 2 2 a i 5 INOA wn on x Publication no 8510253500 Issue 1 August 2009 86 of 88 Varian 440 Fraction Collector Service Manual 8 3 Assembly Loom 3 way Valve Place on valve Varian P N 01 108248 00 2off _ 350 mm DO NOT Shrink heat shrink tube SK1 NOTE 1 2 te WIRE SIDE VIEWS 1 2 Publication no 8510253500 Issue 1 August 2009 87 of 88 Varian 440 Fraction Collector Se
72. ly covers Remove the probe arm assembly covers as follows 1 Remove the two M4x10 self tapping screws and the two M3x12 screws 2 Remove the front cover from the probe arm 3 Manoeuvre the probe arm about the theta axis until the lower edge of the top cover is fully exposed Unclip the top cover from the probe arm and x axis cover 4 Rotate the probe arm to gain access to the rear cover mounting screws 5 Remove the four M4x10 self tapping screws and remove the rear cover from the probe arm Install the probe arm assembly covers as follows 1 Position the rear cover on the probe arm align the mounting holes in the centre plate and secure with the four M4x10 self tapping screws Take care not to strip the threads from the mounting posts in the rear cover when tightening the screws 2 Rotate the probe arm until the rear cover is located behind the x axis cover 3 Clip the top cover into position against the x axis carriage 4 Rotate the probe arm to engage the lower edge of the top cover behind the rear cover 5 Position the front cover over the probe arm assembly and align the mounting holes then secure the front cover with the two M4x10 self tapping screws and the two M3x12 screws 5 10 3 X axis cover Remove the x axis cover as follows 1 Remove the rear panel 2 Remove the four M4x20 screws securing the x axis cover to the column 3 Slide the probe arm to the end of its travel 4 Carefully slide the x a
73. m options mentioned above 3 1 2 1 Connecting the 440 LC Fraction Collector to an Existing or New Varian Modular HPLC System with Galaxie Software There are two ways to connect your fraction collector e Fraction collector to computer e Fraction collector to MIB800 or MIB850 Once the fraction collector is connected to the computer or MIB skip to Section 3 2 1 Page 27 to configure your system in Galaxie 3 1 2 2 Connecting the 440 LC to the Computer To connect your Varian 440 LC to the computer 1 Plug one end of the supplied RS 232 cable into the instrument communication port 2 Connect the other end of the RS 232 cable to a serial port on your computer Ensure that the correct communications parameters are set for both the Varian 440 LC and the computer in the Galaxie software The required instrument communication settings are listed within Table 1 Only the Baud rate setting can be altered via the instrument software The instrument software must also be set to the corresponding values Table 1 Communication parameters for Varian 440 LC Baud rate Data bits Parity 20 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 3 1 2 3 Connecting your 440 LC to an MIB800 or MIB850 To connect your Varian 440 LC to the MIB 1 Plug one end of the supplied RS 232 cable into the instrument communication port 2 Connect the other end of the RS 232 cable to a conversion cable part
74. mber E 8 a 5 s feo An alternate configuration qd dl Sg dA Q is to connect the manual Lt atl ti S kga i ela alaa x injector switch to the detector 2 lt A 2 2 o Startin and Startin inputs via the 02 101865 90 sync interface board r J 3 wire LC interconnect cable This loom is shipped with the manual R007200141 or 01 107441 00 injection valve 8 x 3 wire LC interconnect cable r R007200141 or 01 107441 00 5 g 3 lt s 440 LC Fraction Collector 5 02 102238 00 Poe i i E ika Fraction collector mt Ps Pe fea es ea Rs Status relays 5 relay outputs E El a al al al 23 Relay A reserved f 24V 3 a 8 E a a From the LC analog signal for internal divert Gre o 332 59 8 cable plugged into J14 on valve drive a aq sll lol BE the 325 or 335 detector Relay B a lt 4 lt tc Stop pump rela Be ie E rs Event indication SLAY Td relay From the 325 335 Relay rie Interface board plugged into non GND J4 on the detector Digital inputs Start Siar Stat Start Input 3 Request Start Rivarce Advance Input 2 Request Advance Advance Advan 2 Input 1 Controls divert valve VAN vance sha si Valve Valve vo Fraction Collector I O Ada T C Female socket Male socket 02 102338 00 Fraction collector digital inputs 440 LC Fraction Coll
75. measures may be required to correct the interference 1 7 11 CE Compliant Products The fraction collector has been designed to comply with the requirements of the Electro magnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Varian has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with the Directives is indicated by e The CE Marking appearing on the rear of the product and e The documentation package that accompanies the product that contains a copy of the Declaration of Conformity This Declaration is the legal declaration by Varian that the product complies with the Directives and also shows the EN standards to which the product was tested to demonstrate compliance It is also signed by Varian s Authorized Representative in the EU and by the representative of the manufacturing plant Publication no 8510253500 Issue 1 August 2009 7 of 88 Varian 440 Fraction Collector Service Manual 1 8 1 8 1 1 8 2 1 8 3 1 8 4 8 of 88 Service Philosophy Varian Customer Support Policies 12 months though this may vary according to location Hardware 5 years from date of last unit manufacture After this support period time parts will be provided if available Service Policy In the normal course
76. not exhaustive so if you cannot correct the problem using the information provided you should contact your Varian representative Information is provided on the following e Fractions not correlating to the tick marks on the Galaxie software e Liquid is not delivered to the tubes e Probe hits the top of the tubes or is not low enough e The arm is not moving correctly in the X theta or Z axes e The fraction collector shows offline in Status Overview e The green indicator on the front of the fraction collector is off e The CPU activity LEDS on the rear of the fraction collector are off e Fraction collector is not initializing e Error status LEDs are showing an error Warning Shock Hazard Access to the power supply unit and components requires removal of the main housing This should only be carried out by a Varian representative ALWAYS replace the fuses located in the pull drawer of the fraction collector input switch unit with those of exactly the same type and rating specified Symptom Possible Cause Suggested Remedy Fractions not Delay volume incorrect Determine the correct delay volume and enter it into the correlating to the tick System Parameters or Console software See Section 5 6 marks on the Galaxie Page 40 software Liquid is not delivered Tubing is clogged Check the tubing for blockages and replace if to the tubes necessary See Section 5 8 Page 42 Probe is clogged Clean the probe See Section 5 2 2
77. nsure that one idler pulley is located on each of the three spigots provided on the z axis centre plate and one on the spigot on the tensioning idler Ensure that the idler pulleys rotate freely Route the timing belt over the idler pulleys and the z axis carriage Loosen the screw retaining the idler pulley to allow the idler spring to adjust the tension on the timing belt Tighten the screw Check that the z axis carriage moves freely through its full travel along the z axis shafts Secure z axis brace plate to the z axis centre plate using the three M3x12 screws Install the z axis PCB Install the probe arm front cover 5 11 2 X axis timing belt Remove the x axis timing belt as follows 1 2 3 Remove the x axis cover Remove the probe arm front cover Turn the M3x6 screw retaining the tensioning idler out one full turn Move the tensioning idler just enough to release the pressure on the timing belt Take care not to stretch the idler spring Remove the timing belt from the idler pulley Remove the two M4x10 self tapping screws that retain the belt from the x axis carriage block Publication no 8510253500 Issue 1 August 2009 45 of 88 Varian 440 Fraction Collector Service Manual 8 Remove the belt clamp from the timing belt 9 Remove the timing belt by sliding the belt through the slot in the column chassis Install the x axis timing belt as follows 1 Check that the length of the timing belt
78. o the tube restraint in the Z axis slider Allow for a little extra length in the tubing to prevent kinking 7 Feed the tubing through the retaining ring at the underside of the housing The retaining ring is split to allow you to slide the tubing into it 8 Screw the tubing fitting into the bottom port of the valve Note It is important to ensure that the length of the tubing allows the probe arm to move freely in all axes If it is too tight it will restrict movement and may cause movement failure If it is too loose the probe arm may become tangled in the tubing as it moves It is also important to keep the tubing length to a minimum to avoid a large delay volume Publication no 8510253500 Issue 1 August 2009 39 of 88 Varian 440 Fraction Collector Service Manual 5 6 40 of 88 Note 9 Move the probe arm to the front and back of the fraction collector while rotating the probe arm fully to check that the tube is completely free to move It is also important to keep the minimum length of tubing to minimize the delay volume 10 Manually position the probe arm at the midpoint along the X axis 11 Rotate the probe arm through its full extent to check that the tubing is free to allow full movement in all directions 12 Determine the length of tubing for the sample line to the HPLC system It is important to keep the minimum length of tubing to minimize the delay volume 13 The Tefzel tubing is secured by the black tu
79. of the sample line from the HPLC system In port 2 of figure 5 screw in the fitting with Tefzel tubing Connect the other end of this tubing to the HPLC system s detector outlet or to the back pressure restrictor if one is present This tubing should be as short as possible In port 1 of figure 5 screw in the fitting with Teflon tubing Put the other end of the tubing into your waste container 3 1 1 4 Installing the Sample Racks Fit the pegs on the rack into the holes on the rack location mat if you are using the standard racks The rack closest to the rear of the fraction collector is considered rack number one If you are using the fraction collector rack Type 128 15 x 45 mm OD tubes 4 mL vials or Type 200 12 x 32 mm OD tubes 2 mL vials slide the extended portion on the bottom of the rack into the long slit on the rack location mat l Figure 6 Rack location mat Where 1 Cut outs at the side and rear of the rack location mat Holes for standard tube racks 3 Holes for the fraction collector rack 3 1 1 5 Rack Orientation The image in figure 7 shows an example of where the first sample is delivered to and the orientation of the standard racks provided with the fraction collector Publication no 8510253500 Issue 1 August 2009 17 of 88 Varian 440 Fraction Collector Service Manual Where 1 Rear of the fraction collector AA AA AI 2 First sample dispensed in this 000000000000 position ra
80. olour deterioration and belt tension e The fraction collector requires little or no lubrication throughout its lifetime however lubrication of the x axis shaft should be checked periodically 5 2 2 Cleaning the Probe Run washing solution compatible with your HPLC system and current solvent through the fraction collector tubing If it is completely blocked replace the tubing Sonicating the probe and tubing may help clean any particulates out of the tubing If sonicating the probe and tubing assembly does not remove the blockage replace it Publication no 8510253500 Issue 1 August 2009 33 of 88 Varian 440 Fraction Collector Service Manual 5 2 3 5 2 4 34 of 88 For information on how to remove and replace the probe please see Section 5 5 on page 37 Cleaning the Spill Tray and Rack Mat he EN lu To clean the spill tray and rack mat 1 Turn the fraction collector off Remove the racks Lift up and remove the rack mat Carefully lift up and remove the spill tray Clean the spill tray and rack mat Dispose of any waste in the appropriate manner oar w hd Warning Chemical Hazard Inhalation Hazard Fire Hazard Tubing may contain residual chemicals which may be harmful if you come in contact with them Some solvents may have flammable properties To avoid injury or damage to equipment consult the MSDS and always follow appropriate safety guidelines when using chemicals and solven
81. ove To Point A button Figure 48 and then using the X amp Y movement buttons Figure 48 align the probe so that it rests right above the point A dimple Figure 51 in the tool Do not forget to adjust the Move_z Figure 48 depth adjustment to avoid running the probe into the alignment jig Remember that fine adjustment of the probe can be made by changing the Increment pull down menu Figure 48 NINY Figure 51 Point A on the Alignment tool 4 Once the probe is positioned over the point A dimple then select the Capture Point A button Figure 52 Publication no 8510253500 Issue 1 August 2009 79 of 88 Varian 440 Fraction Collector Service Manual U Calibration Com 1 9600 AIM3000 mode Varian Lower Plane Calibration Upper Plane Calibration Calibration Points A and B must lie on the same Y value Initial move_t0_A Initial move t0 uf hoo fioo Set Point A 16 8 113 5 Clear X and Y Offsets Clear X and Y Offsets SetPointB 366 8 113 5 Move To Point amp ETE TTPO A Set Point C 266 8 213 5 Capture Point A Capture Point A SetPointD 266 8 13 5 Move To Point B Move To Point E D LE Capture Point B Cetera ua ji Calibrate P Calibration Check Calibration Check Move To Point C Move To Point C Move To Point D Move To Point D Ensure the last movement of Z is a downward stroke Use number keys to move the Autosampler NumLock i e I2m_xtrans l2m_ytrans move rad move t0 eny 90 env_t180 en t 270 fo
82. owing an error 7 1 Reset Procedure The reset procedure identifies problems with the movement of the fraction collector on the X and Theta axes If the unit successfully completes the reset procedure then the motor s operation and the action of the carriages is correct Reset is initiated by depressing the recessed button A small pointed device is required to depress the switch on the connection panel Publication no 8510253500 Issue 1 August 2009 63 of 88 Varian 440 Fraction Collector Service Manual 7 2 Self Test Seguence The self test sequence tests that the fraction collector functions on X Theta and Z axes by moving through the standard and sample positions for the currently loaded rack settings It does not test the serial communications If the self test sequence is successful it is only necessary to check the serial port connection to the instrument PC 7 3 Error Status Indicator Codes Error indicators have been provided to give a quick view of the status of the fraction collector and of the possible cause of incorrect functioning of the unit Refer to image above for the location of the error indicator LEDs The error indicators are a line of blue LEDs on the side panel of the fraction collector They are used to provide a visual indicator of an error that has occurred with the fraction collector Other more extensive error reporting is available via the software commands The error indicators illuminate
83. p 58 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 12 Grubscrew 1510250600 14 Screw M4 x 10 self tapping 1510251100 15 Clip 10 mm shaft retainer 20 Spacer theta axis motor 22 Pulley idler 26 Pulley drive z axis 28 Encoder wheel hub theta 29 Encoder wheel hub z axis x axis BOa Belt timing 500 mm B4 Lubricating fluid 200cs Publication no 8510253500 Issue 1 August 2009 59 of 88 Varian 440 Fraction Collector Service Manual 6 5 Probe Arm X axis 1 7 10 17 18 14 15 7 10 13 16 Description Partnumbar a Assy cable theta axis FFE 0110728000 B Assy cable z axis and x axis FFG 0110728100 assy pw x axis encoder 0210185190 6 lamp theta cable 0810168100 Bolenoid Mouni 77220810212500 i oI 60 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 6 6 Special tools ID Description Parinumber 1 Posidrive screwdriver No 2 7210027900 oe p Tool accuracy confirmation ig SP53 7211088300 B froo calibration probe SPS3 7211094800 a D SPS autosampler service raining 8510210000 Publication no 8510253500 Issue 1 August 2009 61 of 88 Varian 440 Fraction Collector Service Manual 7 Diagnostics and Troubleshooting This is a quick guide to identifying and correcting possible problems with your fraction collector The list of problems is
84. replace the fuse only with the same type and rating specified Replacement fuses are available from the Authorized Service Agent Pinching Hazards The fraction probe assembly uses high torque motors that are strong enough to cause injury if fingers become jammed in the transport mechanism Do not attempt to change or remove fraction tubes or trays while the transport is in motion Never place fingers behind the fraction probe assembly while a sequence is running Your fingers may become jammed between the assembly and the frame of the fraction collector if the transport mechanism moves in a direction that you are not expecting Before servicing the transport mechanism make sure the fraction collector is unplugged and turned off Panels and Covers Hazards Other All panels or covers that are retained by screws on the fraction collector may be opened only by Varian trained Varian qualified or Varian approved Field Service Representatives FSRs Consult the manuals or product labels supplied with your PC monitor and printer plotter to determine which parts are accessible Precautions Use of the fraction collector may involve materials solvents and solutions which are flammable corrosive toxic or otherwise hazardous Careless improper or unskilled use of such materials solvents and solutions can create explosion hazards fire hazards toxicity and other hazards that can result in death serious personal injury or damage to equipm
85. rian 440 Fraction Collector Service Manual 4 Upon clicking the Create System button the specified system will be created in Galaxie Make sure you acknowledge the pop up x l The system ModularInt has been created Once you have configured your system go to Section 3 3 in this manual for instructions on how to determine the volume delay and probe depth 3 2 2 Configuring Fraction Collector with 440 LC console Open the Varian 440 LC console software In the opening window select the COM port to which the fraction collector is connected and click the Connect button E Varian 440 LC Console Ele Help Setup Monitor RS232 COM Port m y Status Offine Serial Number j Flow Settings Volume Delay secs amp Rack amp Tube Settings Rack Type Probe Depth mm Max Tube Time mins Movement Settings Start Action Probe Movement Linear Serpentine Divert Valve between moves Rack Position 1 After last tube Rack Position 2 After last tube G Rack Position 3 After last tube amp Factory Defaults Upload Once connected all available setup fields become accessible EJ Varian 440 LC Console File Help Setup Monitor RS232 COM Port Gi M Status Connected Serial Number Cannot Flow Settings Volume Delay secs 89800 Rack amp Tube Settings Rack Type Probe Depth mm Max Tube Time mins amp Movement
86. rovided to help the user operate the instrument safely Read each safety topic thoroughly before attempting to operate the instrument and ALWAYS operate the fraction collector in accordance with these safety practices Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 1 7 2 Electrical Hazards Exposure to high voltages can cause severe skin damage while close contact with the electrical components can result in severe heat burns to the skin and an electrical discharge which may cause death severe electric shock or sub surface skin burns The fraction collector has been carefully designed to operate safely and effectively when using components that conform to Varian s design criteria Use of non approved components in the fraction collector may render the system inoperative and or hazardous lt may also invalidate the warranty on the instrument Use only related components supplied or authorized by Varian e Disconnect the instrument from all power sources before removing protective panels to avoid exposure to potentially dangerous voltages Panels or covers which are retained by screws may be opened ONLY by Varian trained Varian qualified or Varian approved Customer Service Representatives Consult the manuals or product labels supplied with your PC to determine which parts are operator accessible e When itis necessary to use a non original power cord plug make sure the replacement
87. run Yarian Modular HPLC System Galaxie Driver The InstallShield R Wizard will install varian Modular HPLC System Galaxie Driver and 440 LC Control on your computer To continue click Next WARNING This program is protected by copyright law and international treaties License Agreement Please read the Following license agreement carefully GALAXIE Software License Agreement This is a legal agreement between you an individual or an entity the end user and Yarian Inc By installing the software on a computer you are agreeing to be bound by the terms of this agreement If you do not agree to the terms of this agreement promptly return the software package and all accompanying materials 1 APPLICABILITY OF LICENSE This license agreement applies to the GALAXIE SOFTWARE products named on the corresponding Galaxie Software Serial Number Card s and to any OF Installshield 3 Enter the customer information and a valid license number Te Yarian Modular HPLC System Galaxie Driver InstallShield Wizard 1 WI xi Customer Information Please enter your information HPLC Service InstallShield Publication no 8510253500 Issue 1 August 2009 25 of 88 Varian 440 Fraction Collector Service Manual 26 of 88 Depending on whether you are using Galaxie or not select what you want to install i Yarian Modular HPLC System Galaxie Driver InstallShield Wizard Custom Setup
88. rvice Manual 8 4 Block Diagram AUX X Axis FFC X Axis RS232 PCB Motor MAIN 6 Axis 8 Axis PCB FFC Motor PCB FFC Z Axis Z Axis PCB Motor Orientation Sensor 100 240 Y 50 60Hz POWER LED 240 1 84 75W Functional blocks e Main PCB Main control PCB 100 240 V Power input drives all three axes stepper motors contains upgradeable firmware using the MDS software Can also connect one Accessory PCB to drive internal device such as wash pump e AUX RS232 Auxiliary RS232 Communications Port for external devices such as a diluter or peristaltic pump e HOST RS232 Host communications port for communication to the AIM3300 for control or diagnostics Digital IN Relay OUT 8 Digital inputs 4 Relay outputs to control External devices FFC Flat FLEXIBLE Cable Provides communication and power to other modules X axis PCB Encoder PCB for the x axis motor used for collision detection Z axis PCB Encoder PCB for the z axis motor used for collision detection axis PCB Encoder PCB for the theta Axis motor used for collision detection and also has a sensor to determine probe arm orientation on start up only 88 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Publication no 8510253500 Issue 1 August 2009 89 of 88
89. s 8 Determine the volume delay See Section 5 6 TM 5 9 Replacing the Fuse Replace a blown fuse with one of the same type and rating Note Always check the information printed on the back of the fraction collector for the correct fuse type as this is the most up to date Warning Electrical Shock Fire Hazard Use of an incorrect fuse poses a fire hazard and or increases the risk of an electrical shock To prevent reduced safety protection or unwanted fusing always ensure that the marking on the fuse matches the screening shown adjacent to the voltage selector switches 42 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual To check change the fuse 1 Disconnect the Varian 440 LC from the mains power supply 2 Use a thin flat blade screwdriver or similar tool to pry open the cover of the fuse compartment which is located on the power supply unit near the bottom of the connection panel on the rear of the Varian 440 LC r 100 240V 10 75W 50 60Hz 1Hz Flat blade screwdriver Cover of fuse compartment 5x20mm IEC 127 Sht 5 Correct fuse information Figure 19 Opening the flap of the fuse compartment 3 Slide out the fuse holder from the compartment Flat blade screwdriver Fuse Fuse holder Cover of fuse compartment Figure 20 Sliding the fuse holder out of the compartment 4 Check that each fuse is of the correct type
90. s These overlays are designed to improve the location of 18 mm OD and 16 mm OD tubes in the respective racks eseription Kit Rack Conversion for Type 40 rack 18mm OD 6610026000 holes Kit Rack Conversion for Type 60 rack 16 mm OD _ 6610026100 holes 6 1 3 Tubing and Probes Description Part number Kit probe 0 020 ID SS Sleeved 440 LC 9910130800 6 1 4 Miscellaneous Spares esoription i 710051100 bag of 125 tubes raction collector spill tray 6610025100 rolley cart for the fraction collector Features 9910056300 UO o lt D x o D D 0 Cc io D n D 3 3 O 0 x lt N o 3 3 D Q y O Q A sed a D gt oO D n seb a ro oO lt n gt D e n gt fe MW Q D mL screw top wide opening clear glass vials 0392611549 i 0392611602 use 5 A 440 LC FC 1910009100 use 3 15 A 440 LC FC 1910010700 _ D x amp N 3 3 o 3 3 0 a Q D ke 72 gt v 4 TI m QO O D 5 2 pi 3 a Q g oO gt e xe w 3 o D Q je n n lt v n ol gt A Ji 3 3 k ao T TH 3 3 S gadi Q O gadi O n a U Ca TI m 2 A Oo D D n Publication no 8510253500 Issue 1 August 2009 55 of 88 Varian 440 Fraction Collector Service Manual 6 2 Covers D Pescripton art number oo Bovertop bororeeroo _ B3 over probe aim ba
91. s centre plate using the two M3x6 screws and star washers Install and check encoder wheel alignment Install the z axis belt Install the probe arm covers 5 21 Install and check encoder wheel alignment BE x ES ms lt 1h Total width Diode symbol Figure 27 Encoder wheel alignment 1 52 of 88 Check the position of the encoder wheel The clearance between the encoder wheel and the side of the encoder sensor bearing the diode symbol should be less than half the total gap Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 9 22 5 23 5 24 5 25 2 Tighten the grub screw to secure the encoder wheel to the stepper motor shaft Side of the sensor with the white diode symbol Figure 28 Encoder wheel alignment Note The encoder sensor bears the symbol for a diode on one side Ensure the position of the encoder wheel is closest to this side the closer the better However ensure that the encoder wheel does not rub on the sensor Remove the z axis shafts 1 Remove the probe arm covers 2 Rotate the theta arm to gain access to the z axis shafts 3 Slide the z axis carriage to the bottom of its travel 4 Carefully slide the two z axis shafts through the holes in the upper tab of the z axis centre plate and the z axis carriage Install the z axis shafts 1 Carefully slide the two z axis shafts through the holes in the upper tab of the z axis centre pl
92. s in the spill tray 3 Lower the spill tray until the front edge rests on the front of the base support tube 4 Check that the spill tray is firmly fitted Note The spill tray must be firmly in position so that it does not move 14 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 3 1 1 2 Installing the Rack Location Mat The rack location matis installed on top of the spill tray To fit the rack location mat There are cut outs in the sides and the rear of the rack location mat The cut outs slot over the raised locating tabs that are molded into the spill tray to ensure proper alignment See the image above for details 1 Position the rack location mat over the spill tray and press the mat onto the locating tabs 2 Check that the rack location mat fits firmly in place by trying to move it side to side There should not be any movement If the rack location mat is loose refit it 3 If it does not fit properly contact your local Varian office or representative 3 1 1 3 Installing the Probe and Tubing For image details see Figure 1 Page 13 To install the sample probe and probe tubing 1 Turn the fraction collector off 2 Slide the Z axis slide to the top of the probe carriage 3 Manually rotate the probe arm so that it can be easily accessed 4 See Figure 3 below Disassemble the probe kit by unscrewing the nut holding the metal probe from the bottom of the
93. t appear in conjunction with warnings in this manual or on the fraction collector The hazard they describe is also shown 1 7 8 1 Warning A triangular symbol indicates a warning A Warning message appears in the manual when failure to observe instructions or precautions could result in death or injury Symbols depicting the nature of the specific hazard are also placed alongside warnings Warning Electrical shock Ensure that the Fraction Collector is always connected to the mains supply protective earth Ensure to position the Fraction Collector so that it is easy to operate the mains power switch or to remove the power cord The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows Publication no 8510253500 Issue 1 August 2009 5 of 88 Varian 440 Fraction Collector Service Manual Read all warnings and cautions carefully and observe them at all times A gt Broken glass Corrosive liquid Electrical shock Eye hazard Fire hazard Heavy weight danger to feet gt Heavy weight Moving parts danger to hands 1 7 8 2 Caution A Caution message is used when failure to observe instructions could result in damage to equipment Varian supplied and or associated equipment Caution Corrosive chemicals can damage the solvent lines of the fraction collector 1 7 8 3 Note Note A note is used to give advice or information The
94. tab Place the correct racks and tubes on the fraction collector The fraction collector will wait for the input signal to start if selected or click Collect to start the fraction collector using the settings uploaded in Step 5 The current rack tube and divert valve positions are displayed on the Monitor tab The rack display shows filled tubes and the current tube A ONAA 9 To manually move the probe click Next Rack Next Tube or Home 10 To change the divert valve position click either Valve Collect or Valve Drain The Monitor Log on the Monitor tab records the tube and rack location of each fraction from each sample It is recommended that you save this Log by clicking Save Log Click Clear Log to delete the log information The Comms Log records every communication between the fraction collector and the computer If an error occurs it is recommended you save the Comms Log Click Clear Log to delete the log information Tip To have the Console window stay on top of all other windows click File gt Stay on Top Tip The Console software window is resizable Drag the sides until just the Stop Collect Next Tube and progress bar are showing for a basic fraction collector Console panel 32 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 5 Maintenance Procedures 5 1 Introduction The 440 Fraction Collector has been designed for low maintenance and there ar
95. tall the x axis stepper motor 50 5 18 1 Remove the x axis stepper motor 50 5 18 2 Install the x axis stepper motor 51 5 19 Remove and install the theta axis stepper motor 51 5 19 1 Remove the theta axis stepper motor 51 5 19 2 Install the theta axis stepper motor 51 5 20 Remove and install the z axis stepper motor 52 5 20 1 Remove the z axis stepper motor 52 5 20 2 Install the z axis stepper motor 52 5 21 Install and check encoder wheel alignment 52 5 22 Remove the z axis shafts 53 5 23 Install the z axis shafts 53 5 24 Remove the power supply 53 5 25 Install the power supply 53 6 Spare Parts 55 6 1 Consumables 55 6 1 1 Sample Racks 55 6 1 2 Rack Overlays 55 6 1 3 Tubing and Probes 55 6 14 Miscellaneous Spares 55 6 2 Covers 56 6 3 Control Electronics 57 6 4 Probe Arm 58 6 5 Probe Arm X axis 60 6 6 Special tools 61 7 Diagnostics and Troubleshooting 62 7 1 Reset Procedure 63 7 2 Self Test Sequence 64 7 3 Error Status Indicator Codes 64 7 4 Probe Arm Jam 65 7 5 Communications 65 7 6 Failure to Initialize 66 7 7 MDS manufacturing amp diagnostics software 66 7 71 Before starting 66 Publication no 8510253500 Issue 1 August 2009 v Varian 440 Fraction Collector Service Manual vi 8 1 8 2 8 3 8 4 7 7 2 Reload Fraction Collector Calibration Coefficients 7 7 3 Calibrating the 440 LC Fraction Collector Electronic and mechanical schematics Fraction Collector inter wiring Fraction Collector Power board Assembly Schematics
96. the probe assembly 3 Probe mounting block 4 Lower knurled mount nut on the probe assembly 1 5 Ferrule holding the metal probe 2 3 4 5 Figure 15 Probe details Warning Moving Parts The probe arm of the Varian 440 LC can move without warning and so poses a potential hazard due to its moving parts Always switch off the power to the Varian 440 LC before installing or removing the sample probe Warning Chemical Hazard Inhalation Hazard Fire Hazard Tubing may contain residual chemicals which may be harmful if you come in contact with them Some solvents may have flammable properties To avoid injury or damage to equipment consult the MSDS and always follow appropriate safety fu guidelines when using chemicals and solvents and always wear appropriate safety equipment and clothing To remove the sample probe and probe tubing 1 Turn the fraction collector off 2 Move the Z axis slide to the top of the probe carriage 3 Manually rotate the probe arm so that it can be easily accessed 4 Unscrew the tubing from the divert valve position 3 Publication no 8510253500 Issue 1 August 2009 37 of 88 Varian 440 Fraction Collector Service Manual To waste From detector outlet or back pressure restrictor To the probe Figure 16 Divert valve tubing positions 5 Remove the tubing from the tube restraint in the Z axis slider 6 Remove the tubing from the split retaining ring at the underside of
97. the probe to catch any liquid to avoid spills which may cause damage to your instrument a If you are using Galaxie software double click the Fraction Collector tile in the Status Overview and then click Switch valve on b If you are using the Varian 440 LC Console click the Monitor tab and then click Valve Collect Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual 4 Run the flow until there is water or compatible solvent for your system from the detector to the fraction collector probe 5 Stop the flow 6 Place the empty weighed glass cylinder under the valve to collect all liquid that comes out Disconnect the outlet tubing at the detector 8 Connect the syringe to the end of the tubing 9 Using the syringe push air through the tubing until air exits the probe 10 Weigh the cylinder with liquid and subtract out the weight of the cylinder to get the weight of the liquid 11 If you are using water at room temperature then 1 uL 1 ug of water If your liquid weighed 300 yg then your delay volume is 300 uL 12 Enter the volume into the volume delay field in either c The system parameters tab in the device settings in the Galaxie software Click Save System Parameters d The 440 LC Console software on the Setup tab Click Upload to send the information to the fraction collector 5 7 Determining the Correct Probe Depth Caution Do not set a probe
98. to show the binary error code for the fault E4 is the least significant bit in the binary code If an error occurs the binary error code is indicated and an error message is sent to the Galaxie software for example Error 7 0111 Theta axis position error Refer to the table below for the error indicator codes op pp p Not op pf h Rewa FoS 1 2 Program memory check sum error Contact your Varian representative 1 1 3 Configuration memory check sum Contact your Varian error representative 1 4 RAM Test error Contact your Varian representative o ft e Rewa F o o 1 X axis position error The X axis Troubleshoot the probe arm motor was commanded to move but did not reach the desired position 1 7 Theta axis position error The Theta Troubleshoot the probe arm axis motor was commanded to move but did not reach the desired position Z axis position error The Z axis motor Troubleshoot the probe arm was commanded to move but did not reach the desired position o Rema oOo o o fema Fo 1 11 X axis movement detected without the Troubleshoot the probe arm motor being commanded to move 12 Theta Axis movement detected Troubleshoot the probe arm without the motor being commanded 1 1 1 1 1 1 Z axis movement detected without the Troubleshoot the probe arm motor being commanded to move C e AI e WAA 64 of 88 Publication no 8510253500 Issue 1 August 2009 1 1 o p
99. ts and always wear appropriate safety equipment and clothing Lubrication Lubrication of the x axis is designed to reduce friction between the carriage block and the left x axis shaft providing smooth travel of the x axis carriage If vibration or erratic movements of the x axis carriage are observed we recommend you check and lubricate the lubrication pad To lubricate the single x axis shaft follow the guidelines below 1 Remove the rear panel Refer to Section 5 3 Removal and replacement procedures 2 Remove the x axis Cover Refer to Section 5 3 Removal and replacement procedures 3 Remove the theta axis PCB Refer to Section 5 3 Removal and replacement procedures 4 Rotate the theta axis driven gear to expose lubrication well 5 Add 1 mL of DOW CORNING 200 R FLUID 200 CS into the well 6 To complete reverse the above procedure Warning Shock Hazard Death serious injury or painful electrical shock can result by contact with the 440 LC circuits devices and components This instrument contains electrical circuits devices and components operating at dangerous voltages Warning Moving Parts The probe arm of the Varian 440 LC can move without warning posing a potential hazard due to its moving parts Always switch off the power to the Varian 440 LC before installing or removing parts Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual
100. ured according to a Quality system certified to ISO 9001 The guaranteed specifications are based on the 3 sigma statistical confidence level of the final acceptance tests performed at the factory Safety Practices and Hazards Information on safety practices appears throughout the documentation both hard copy and on line provided with your instruments and accessories Before servicing the instrument or accessories you must thoroughly read these safety practices If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Observe all relevant safety practices at all times General Safety Information Operation of a Varian 440 fraction collector involves the use of hazardous materials including corrosive fluids and flammable liquids Careless improper or unskilled use of this fraction collector can cause death or serious injury to personnel and or severe damage to equipment and property The fraction collector incorporates interlocks and covers that are designed to prevent inadvertent contact with any potential hazards If the instrument is used in any manner not specified by Varian this protection may be impaired It is good practice to develop safe working habits that do not depend upon the correct operation of the interlocks for safe operation It is essential that no interlock or cover is bypassed damaged or removed The safety practices described below are p
101. w Publication no 8510253500 Issue 1 August 2009 81 of 88 Varian 440 Fraction Collector Service Manual 82 of 88 U MDS Manufacturing amp Diagnostics Software Com 1 9600 AIM3000 mode Varian ELO File Communication Port View ESSI Help General Details ___pjagnostics Ctrl D __ Installed Accessory Boards come oi eee os IOS Calibration Ctrl C oard Version Details Serial Number EL07073734 Variables Ctrl y Main Board Details Sample Ctrl S 8 Not Used Model SPS3 AccuracyArray Ctrl 4 fe 3 Z Accuracy Ctrl Z wA 7 Not Used System Firmware PIR Firmwar et 6 Not Used Version V1 20 PR C Perpendicularity Ctrl P 5 Not Used i Scientific Odometers Ctrl O 4 Not Used System ID 000010515 Config Checksum af9b3df 3 Probe Monitor Relay 2 AIM3300 Defaults Loaded at reset 1 4IM3200 Defaults Loaded at reset Options Program Checksum a502091d Test Mode Swit swz 1 Test Mode KUE Relay Status 4 E DP DIP Status 2 Test Mode Test 1 Test 2 STLED Status Si Be ey Test 3 Digital Status Represents High or ON 5 Baud Rete ON 19 2K OFF 9600 9 gan a i i maU J ao 6 ON AIM1250 OFF AIM3000 Mode Spfesers Low or 7 MRS Expiry Timer ON 4min OFF 30s 8 ON MRS OFF Fixed Wash Refresh Manufacture Diagnostics Exit Sent stepper read Received OK 916 891 011 Port 1 Open 11 09 2008 10 14AM Figure 56 Main Page of
102. xis cover off 5 Remove the x axis carriage cover Install the x axis cover as follows 1 Slide the probe arm to the end of its travel 44 of 88 Publication no 8510253500 Issue 1 August 2009 Varian 440 Fraction Collector Service Manual Align the x axis cover with the x axis shafts and carefully slide the x axis cover into position Secure the x axis cover to the column using the four M4x20 screws ensuring that they align with the mounting points in the x axis cover Install the x axis carriage cover Install the rear panel 5 11 Removal and installation of timing belts 5 11 1 Z axis timing belt Remove the z axis timing belt as follows 1 2 3 8 Remove the probe arm front cover Remove the z axis PCB Remove the three M3x12 screws that secure the z axis brace plate to the z axis centre plate Carefully remove the z axis brace plate Loosen the screw retaining the tensioning idler and turn the screw out one full turn Move the tensioning idler just enough to release the pressure on the timing belt Take care not to stretch the idler spring Remove the timing belt from the idler pulleys and the z axis carriage Remove the timing belt from the stepper motor pulley Install the z axis timing belt as follows 1 7 8 Loosen the screw retaining the idler pulley and gently extend the idler spring to allow the timing belt to pass over the idler pulley during installation Tighten the screw E
103. y Practices and Hazards 1 7 1 General Safety Information 1 7 2 Electrical Hazards 1 7 3 Fire Hazards 1 7 4 Pinching Hazards 1 7 5 Panels and Covers Hazards 1 7 6 Other Precautions 1 7 7 Information Symbols 1 7 8 Warnings and Cautions 1 7 9 Color Coding 1 7 10 Federal Communications Commission FCC 1 7 11 CE Compliant Products 1 8 Service Philosophy 1 8 1 Varian Customer Support Policies 1 8 2 Service Policy 1 8 3 Spare Parts Policy 1 8 4 Warranty 1 8 5 OEM Warranty oO O oO O O AN DDO A a FKP AA FP WDM NY NY NY NY NY a 1 8 6 Contract Maintenance 9 2 Pre installation 11 2 1 Weights and dimensions 11 2 2 Electrical specifications 11 2 2 1 Mains supply 11 2 2 2 Fuses 12 2 2 3 External connections 12 Installation Procedure 13 3 1 Hardware Installation 13 3 1 1 Assembling the Varian 440 LC Fraction Collector 14 3 1 2 Connecting Fraction Collector to LC System 20 3 2 Software Installation 24 Publication no 8510253500 Issue 1 August 2009 iii Varian 440 Fraction Collector Service Manual 3 2 1 Configuring Fraction Collector in Galaxie Software 27 3 2 2 Configuring Fraction Collector with 440 LC console 28 3 3 Determining the Volume Delay and Probe Depth 29 4 Operation 31 4 1 Turning on the Instrument and Software 31 4 2 Creating a Method Using Galaxie Software 31 4 3 Controlling Fraction Collector with 440 LC Console 32 5 Maintenance Procedures 33 5 1 Introduction 33 5 2 Routine Maintenance 33 5 2 1 General Clean
104. ystem ID 0000105154c Probe Monitor Relay 4 AIM3300 Defaults Loaded at reset Config Checksum afSb3df1 AIM3200 Defaults Loaded at reset Program Checksum a502091d Test Mode Sut sw Relay Status A E E ID DIP Status Test Mode Jumper Status System Fiimware PIR Firmware Version 1 20 PR C2 07 Copyright C Aji Scientific 2007 DNUS Test Mode S1 LED Status E1 MRS Expiry Timer ON 4min OFF 30s Reserved Reserved Digital Status 2000001010 Represents High or ON ee ON MRS OFF Fixed Wash Refresh Manufactures Cena Baud Rate ON 19 2K OFF 9600 ON AIM1250 OFF AIM3000 Mode Sent aux_read Received OK I Port 1 Open Figure 31 Varian SPS MDS Software Front Page SBYAMDARON gt 8 Insert the customer Details and Serial number of the unit Then click the Diagnostics Button You should now see the screen in Figure 32 U Diagnostics Com 1 9600 AIM3000 mode Varian ELO7073734 Main Board Communication General Communication EF Acc Firmware 9 z o N PIR Firmware 1 20 PR C2 07 Pe a an Copyright C Ai Scientific 2007 Digital Read l D00000 Open Log Send Script Options Close Pos Read 11 63 10 72 0 00 DESA Sent stepperread ea Stp Read 1391 962 0 Move XY Reply 391 962 0 ena Enc Read 391 961 0 u Main Board Settings Move Z Jumper Read m Represents DIP Read Move XY 8 Not Used High or On 1 Test Mode Po ove 7 Not Used mm Represents 2 Test Mode ns n

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