PHM-109 INTRACRANIAL PUMP
Contents
1. indicate ow ine pins af each connector shoul ala Figure 2 2 Connect to MOTOR CONNECTION Connect the cable that extends from the PHN 109 pump shown in Figure 212 to the cannectors on he Multi Channel Commutator indicated in Figure 2 13 Again the connector plugs into the red port and the white connector Inte the white port blac Ines indicate proper pin arentatin Figure 212 Pump Controlling the Pump with MED PC WARNING The 5G 216A cable supplies power to the pump when connected fo MED PC Output therefore it ls not necessary to use the 28 Inverter Connecting the 28 VDC Inverter and the 56 2164 cable to the Pump Controller concurrently may cause equipment damage 6 Using the 50 2184 cable Figure 2 7 connect the MED INPUT connector on the er Figure 2 4 to MED PC Output ump Operating Pump In Manual Mode 6 Connect the 28 VDC Inverter Figure 2 the EXTERNAL POWER connectar on the Pump Controller Figure 24 The pump now ready to operate 11 Jumper Settings Figure 2 15 with Jumper im the 11 Postion The J1 connector inside the Pump Controller Figure 2 15 has a pin jumper whose position can change the function of the pump based on the needs of the user The top four sets of pins 2 1 12 14 and 1 8 reacing dawn the left in igure 2 15 control the pump spe2. PHM 109 INTRACRANI AL PUMP USER S MANUAL 109 Hand Pump TABLE OF CONTENTS Chapter 1 1 Introduction 1 Warnings and Cautions 1 Chapter 2 2 Installation Instructions 2 2 5 Hardware Guide Guise Installation Instructions Jumper Settings Volume Infused per Step Chapter 3 Operating Instructions Pump Travel u Loading the Pump 2 MED PC Controlled Operation Priming the Pump CHAPTER 1 Introduction The PHM 109 Intre Cranial Pump is head mounted nanalter injection system far eivering compounds direty into a rat brain The pump ls mounted on the animals head and the animal can move freely since supparts Ihe pump The short tance between the pump and anima brain allows the system delver consistent volumes The 109 pump controled by stepper motor and can be operated ether manually or through MED PC lt is important tat this manual be read theroughly prior to operating ti equipment Warnings and Cautions WARNING The 5G 216A cable supplies power to the pump when connected fo MED PC Output therefore it Is not necessary to use the 28 VDC Inverter Concurrently Connecting the 28 Inverter and the SG 216A cable to the Pump Controller concurrently may cause equipment damage NOTE Els recommended thatthe maximum number af steps per infusion be 200 The Pump wil nat function propery
3. avels Record the distance ang repeat a leas ten times A Average the distance traveled for each tral 5 Calculate the average volume per step by converting the average distance traveled to cm Then multi that number by the volume per em af the tubing This the average valume infused Divide this number by the number of steps and this the average volum per step For example ithe pump run for 50 steps and the average mm traveled is 4 47 the calculation wil go follows Average Distance Traveled 45 mm 0 450 cm Average Volume infused 0 450 em 2 640 1 188 Average Volume per Step 1188 50 steps 0 024 step Se for the pump in this example the average volume per step Is 0 024 NOTE Ifthe Jumper onthe conector Inside the Pump Cantrller Figure 215 5 in the 2 2 or 22 position the pump will be operating at or step it Is Important to note thatthe volume infused per step wil decrease according 3 Operating Instructions Pump Travel Because of the PHM 109 compact size the amount of volume that can be infused in a ingle injection is limited It Is recommended that the maximum number of steps infusin be 200 Exceeding tis numberof steps may cause the pump to reach the end of travel which may cause damage tothe pump motor Care should be taken to ensure that the pump at the beginning af travel prior to starting infusion as shown below in Figure 3 1 When the
4. ed Operation The PHH 109 Intracranial Pump can be controled easily using NED PC A sample programs shown below In the example above the pump can be run at her 50 100 or 150 steps Each step wil ist ve hundred milisecones NOTE tis recommended thatthe maximum number o steps per Infusion 200 The Pump may nat funcion properly tis number of steps exceeded It should also be noted thatthe setting of the MOTOR DIRECTION switch Figure 23 on the Pump Controller 6 recagrize when the pump Is being contras by MED PC Priming the Pump The Manual Operation function af the 109 Intracranial Pump ls intended to be used nly forthe purposes of priming the pump and tubing It 5 nat possible to accurately Calculate the volume infused when operating manually The 28 VDC Inverter must be Connected tothe Pump Controller in order fr the pump to function in Manual made NOTE The 5G 216A cable supplies power to the pump when connected to MED PC Output therefore connecting the 28 VDC Inverter and the SG 216A to the Pump Controller concurrently may cause equipment damage To operate the pump manual simply press the MANUAL OPERATION button Figure 2 3 The pump will operate for as long this button is held down The direction of the pump can be changed using the MOTOR DIRECTION suite Figure 23
5. ed when operating in manual made The jumper position and the corresponding speed I steps per second are as follows The st of pins labeled are nat used The bottom two sets of ins 21 and 2 2 reading down tne In Figure 215 the step size of the motor The Jumper position and corresponding step size ae as follows No Jumper Full sten 21 22 Step Volume Infused per Step When controling the pump with MED PC te user contol ie number of motor steps per infusion The 109 Intracranial Pump infuses approximately 23 nanalkers Step However A is advisable that each pump be validated prior to use The recommended method for validation 1 Except for a 2mm airspace fil a length of tubing witha colored water colorea with blue vegetable for example and attach it ta the of the syringe needle The 2 mm air space should be placed at the star ofthe line The inner diameter of te tubing must be known 2 Calculate the volume contained in 1 cm 20 mm of tubing Inner D ameter 2 Inner Radius Volume Cylinder Where Lis ine length of tubing which is Lem in his case For example tubing with an inner diameter of 0 56 mm being used then 0 58 mm 2 0 29 mm F Volume contained in 1 em 10 mm of tubing 3 18 0 299 10 264 pl 3 Operate the pump fora known number of steps and measure the distance that the airspace tr
6. if this numberof steps is exceeded 55 will each the fend of ts travel may burn out the pump motor NOTE ifthe Jumper on the connecter sige the Pump Controller Figure 2 15 em the 21 ar 2 2 postion the pump wil be operating at or step Iti important to note that the volume infused per step ull decrease accordingly 2 Installation Instructions Hardware Guide 2 VY Installation Instructions 1 Install the Swivel Assembly Figure 2 5 in the desired locaton on the animal chamber 2 Remove the two screws and the retaining bar at the end af the swivel assembly shown Figure 2 9 Figure 29 End ol 3 Place the Multi Channel Commutator Figure 2 21 inthe slot at the end ofthe Suivl Arm and reinstall the retaining bar and screws as shown in Figure 2 10 Figure 210 Mat Channe on the Swivel Are sing the Controller Cat Figure 2 6 connect MOTOR CONNECTION port on the Pump Contrallr Figure 24 tothe cannectars Mult Channel Commutator Indicated in Figure 211 The end of the controller cable that connects to the CCommutatar ha twa cannectrs one white and one red The red connector should plugged in to the port on the Commutator ndcated in Figure 2 12 ang white The connectors are also marked with blac ins tat
7. pump reaches the end of travel shown in Figure 3 2 it should be cisconnected from the animal primed see the Priming the Pump section ofthis manual m igure 32 End o Pump Br qe Loading the Pump Figure 33 Pump Assembly FR ump Canister Before each session the injector tubing and the pump canister must be filed with the Infusion solution the injector tubing assembly by connecting 1 cc syringe filed withthe Infusion solucion Injec the infusion solution nt the injector being sure that there are ai bubbles present The pump canister can filed inserting the needle Up ofthe syringe into the syringe needle af the pump canister while holding the Canister vertically syringe naedle end down Next careful connect the injector tubing to the syringe needle o the pump canister The canister be connected the hreaged plunger by turing the manual motor 1 2 fll revolute beng sure that the guide screw is in the alignment channel Before connecting the pump to the animal fe recommended that the pump be run tner using the MANUAL OPERATION Button on the Pump Comrallr ar by using MED PC fe step the motor un Sere is lud at he end of In njacton cannula se the Priming the Pump section of this manual Next remove the dummy cannula from the guide and connect the PHM 109 intracranial Pump tothe animal Controll
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