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        MCO-20AIC - Panasonic Biomedical
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1.                                                             L  BL      ESAT NE   T            ee     E ar 51 BR  80Hz  E TES  3      ais          ET                 Po       BALLAST o a  o    inp      gt                                   gt           vi GLOW STARTER Ke t 5  47 427 rom                            FREQUENCY   70 25 9           Y    toC Fa  lt   WX ovt                        CHANGE SWITCH   Mes LUE  a          Xo   hit de e Ng                              lt  o                ONLY ACIOOV   SIDE HEATER  R       dx            tn   BAD4        g  5  Ou Ou                              ee    Y        SIDE MEATER  L    D fil    Y  x  gt  FLANGE HEATER  m E         2575  t 5  F   w CN14                        pey   BOTTOM SIDE HEATER         5 a CN13 ome      Rt 5  8           c    D Y                BOTTOM SIDE HEATER  1   S        is      OVER HEAT        9            uj 9    c        PROTECT RELAY L  BL  Q    xa 9 RLY3 ARBEUL   d  r      cs RLY  UV LAMP RELAY   x               O REMOTE ALARM UV577 Jv        4 a RELAY  a        XRESNUL      z         TENN       Lv  gt  10   lt   PWB BIMETAL       qa     THERMO   lt           4419                 gt  CO 2 0       gt  l 4           5               coe         QUI                                N RLY3DI 2  gt  HEATER    HEATER B        gt  24   77          z  ING      L BL  OQ   e   O     aw                                                2           gt                                    gt  AGITATING   gt  Gand FAN MOTOR
2.            US                                  1                      S703                  TENER                                                E                                   08  35 WilVik                          ow    6                OLAV          209             zc   MR o o o               UJONYHJ        AAWA ZOI                                                            202   NAE DN                           16   i        z    PESE      zi  TIME ur IH                       283  Cae tc           tr                                                    zem Ht           TW                       Se                         M                                                                      tt Ir AS iyo suai v                d ame NLACUONO E  2              R                                          A8t                    81   0832 Giny                                                                                  ane  7  518     313  rni    T                    LU     S Hh            pura                dh               cem      ibus      dein      HMM   RUE             1 cA a                    TEMP  SENSOR    PROTECT SENSOR    025 BOX TEMP SENSOR    OVER HEAT    27               Silk Print                       DP102    DOOR    HEAT J122  DP103                        C  6 18     QPAI2 10                                        Qs     i  Pur E         J05  RH PAN 90 1094    0108   JO7                              2222       559       2  0            amp     mo A   2  
3.         CN4          Contents    Page   Ead     eee 1   Structural specification        2   Performance specification       n 3   Electrical specification   4                                                         7   Temperature calibration        9         calibration      10  Control specification      M H   mw 11  Wiring diagram   nn 26  Circuit diagram      27  Silk Print on PCB  E                                                           28  Connection on PCB  rc xc     29  Tubing diagram  mms 31                     _______ _ __ ___ _  ane 32                                    33  Specification of sensor   mmm 35  Parts layout m 36  How to replace UV lamp  mm 40  How to replace water level sensor  mm 42  TETTE ELEM  mE 43  Mounting procedure of        200        45  Honto stack      49    User s manual  m 51         Connection on PCB        The following shows connections of connector CN  on control PCB    Connector                     00 20    Power transformer for P C B    Power supply for PCB   1     2 AC8 5V    Power supply for air pumps   3 GND  AC36V      4   5 AC18V       Switching power supply Power supply for PCB and         1    2 GND  DC5V sensor                              CO  out put signal     1     2 E  Heat protect sensor   3     4 fel Temp  control sensor To detect the temperature   5     6     sensor box temp  sensor    Analogue out put   1     2 Temp  analogue output To connect external recorder   1     3       analogue output          Switch  Displ
4.        tone CO   valve close   y   temperature indicator     If the upper limit alarm temperature  n  Main heater alarm operates  or if the main heater is        e fa bel 2        abnormality open circuit  or the main heater relay    h P Pune   short circuit  emperature indicator     ir wth the temperature on te               Bottom heater If the bottom heater goes open circuit               with the temperature on the  Intermittent tone  abnormality or the bottom heater relay short circuit  temperature nacar    Door heater If the door heater goes open circuit  E14 is displayed alternately         with the temperature on the   Intermittent tone  abnormality or the door heater relay short circuit  temperature indicator     If the sensor box heater goes open E15 is displayed aiternately  circuit  or the sensor box relay short with the temperature on the              circuit  temperature indicator   Disconnection of If the relay of main heater  bottom E16 is displayed alternately  heater circuit  temperature indicator   Air pump  for sample   the air pump  sampling or auto zero    E17 is displayed alternately  air or auto zero  does not operate  or if there   E  with the temperature on ihe Intermittent tone        valve close   failure something wrong in the pipi temperature indicator   Rap ue  rnewn opens aos                     ies OY E            new UV  amp UV lamp is OFF       The remote TE IS    by pressing the buzzer stop key  BUZZER  as the remote alarm is operated  in c
5.      28  Connection on PCB  rc xc     29  Tubing diagram  mms 31                     _______ _ __ ___ _  ane 32                                    33  Specification of sensor   mmm 35  Parts layout m 36  How to replace UV lamp  mm 40  How to replace water level sensor  mm 42  TETTE ELEM  mE 43  Mounting procedure of        200        45  Honto stack      49    User s manual  m 51    1  PID control with micro computer   This unit has PID control  Proportional Integrate Differential  accurate controls internal  temperature  as well as air sensor system monitoring internal temperature     2  Infrared       sensor installed   Infrared        sensor is not affected by humidity     3  UV sterilization system   Water and the circulating chamber air in the humidifying pan are sterilized by UV lamp   Optional setting of light mode is available     4  Rounded Ball corners   Inside chamber wall made by stainless steel and its corner made rounded type   This made cleaning easier and de contamination     5  Heduce contamination   Inside chamber material is made by anti bacteria SUS   Appearance and durability are almost same with ordinary SUS   Anti bacteria SUS has good effect same as copper alloyed   except for humidity pan     6  Easy of Use  Chamber capacity  195L  Only 900mm height for double laid      Door mount type    is adopted for control panel   Large digital display    key pad   Selectable left or right door opening   This service will be available at local     7  Options   1 
6.     6 03  5 83    14 12 33 zae   5   46        The following shows        density and output voltage characteristics on        IR sensor    Output voltage is amplified by amplifier on main PCB       96   V   96   V   96   V     00   40          28   160   24      10   374   90   28   170   243  3 0  4 0  5 0  B       3 54 2 40         60   30   40   255          o        294   150   25   l o         This output voltage figure are used reference only                                        Fanmotor    Auto zero air pump    Monitoring hole       Filter       DC5V        gas inlet    Power supply    Glow starter    Ballast           Sensor box    Trnsformer  for P C B    Noise filter           sensor    circulation         Filter for PSP    CO   sensor Filter for auto zero           sensor box  temp  control sensor    Thermal fuse       CO  sensor box heater          Outlet          of Sampling  gas for        sansor          Lead wire of water Lead wire of UV lamp socket  level sensor    Buzzer P C B            How to replace UV lamp        When exchange UV lamp  you will need to replace UV              lamp and the socket as a set  sink rubber used for the  socket may be deteriorized   Also at the some time  you will need to replace glow  socket as well     1 Unplug the unit and check that power is not supplied to  the unit  then take the rear cover off   2 Remove the 2 connectors of the lead wire for UV lamp  and lead wire hole insulation material     UV lamp lead wire  connec
7.     The disposal of the unit should be undertaken by appropriate personnel  Always remove doors to  prevent accidents such as suffocation        STACKED MODULE    This unit can be stacked by using the stacking kit  Following shows the procedure for stacking the unit   Consult with a Sanyo representative or agent prior to stacking procedure as such work involves dangers      NWARNING      Select a level and sturdy floor having enough strength for installation of stacked module        Never stack 3 or more units   Take care not to drop or tip over the unit when stacking as this can cause injury or damage of the unit     1  Turn off the power switch and disconnect a plug of each unit     2  The stacking plate A and B are attached on the back of each unit  To prepare the stacking plate A and  B  remove the rear cover by unscrewing 6 screws on the rear cover  See the figure below     3           out the stacking plate    and B by loosening 2 fixing screws on each unit   4  Replace the rear cover and fix it with 6 screws     5  Check that the lower unit is level     Stacking plate             Stacking plate B    Fixing screws    Rear cover      81                      STACKED MODULE               6  Apply the protective sticker enclosed with the unit at each corner      the top of the lower unit to avoid  scratches or damage     T  Fix the stacking plate A at 2 locations on the top front of the lower unit by using 2 screws removed in step  3     8  Remove the front panel on the upp
8.    hinge         SS        Inner door  hinge pin    Earth wire         Door lead wire       Nylon clip    A part details    Outer door hinge installing screw  Fastening torque  over 80kgf cm          screw    4 Preparation to reverse the unit   1  Cap for screw  M   small cap S   cap for lead wire exit  L   inner door hinge  inner door latch  receptor  screws to be reversed     Inner door  latch receptor        Inner door  hinge    Cap L    S  small     M  medium   L  large       5 Place inner  outer door    1  Place the door to the unit  lead wire connector 2 place   earth wire to be put back  and fasten by nylon clip    Be careful that the lead wire will not be too short  when fastening with nylon clip     2  Place a bushing at lead wire exit part and place back  the power panel     3  Place inner door upside down     Inner door hinge pin    LA Outer door hinge installing screw  Fastening torque           80kgf cm    Door lead wire   S                       Door lead wire  exit bushing  Nylon clip       B part details         Mounting procedure of MCO 20GC         Parts list                0 connecting port for 22     tube band  L        CO   valve assy CO   gas pipe    ET      tube band  S            2             assy    Mounting procedure  1 Disconnect the power cable and make sure that the  unit is not supplied with the power     2  Hemove the rear cover by unscrewing the 6 fixing  Screws     Rear cover       Fig  1        45        3         by using the enclosed screw
9.   3      98  5           1   IS TA  5       5                                 5  oO  5      n   lt     10   e        5       D    lt                        3  5               lo   lt  3 O 5 5     M      in  et          D         2 5  3 o  2  o    Natural evaporation with humidifying pan  PID control  Digital display  ON OFF control system  Digital display  Fan assisted  0 3    m  Efficiency  99 97   4 W  Ozone free type  Optical type    Temperature alarm         density alarm  Upper limit temperature alarm  Door alarm  Allowable contact capacity  DC 30V  2A  4 to 6 mm diameter tube  0 03MPaG  0 3kgf cm G  4 3psiG     5 trays  5 sets of tray support  1 gas tube  1 humidifying pan   Stacking plate A and B  fixed on rear of the unit   2 tube bands    Alarm  Remote alarm contact    5         S   lt            9  5  5     2 15      8               5  6  a  o  5 jo          5    5  5  5       lt      9  9  5  2        2      S  5  o 9           5 la          2 iS la          ie           2 o  5     e              lt      9   lt                     connecting inlet  CO   gas pressure    Optional accessory CO   pressure regulator  MCO 100L   Roller base  MCO 20RB     Automatic gas switcher  MCO 20GC   Extra tray  MCO 58ST  including 2 tray supports    Note  Design or specifications is subject to change without notice       Olsl  gt        9      8      t                   o          83                                   PEE        Ambient temperature  5  C to 50  C    P ing ambient te
10.   4                                                         7   Temperature calibration        9         calibration      10  Control specification      M H   mw 11  Wiring diagram   nn 26  Circuit diagram      27  Silk Print on PCB  E                                                           28  Connection on PCB  rc xc     29  Tubing diagram  mms 31                     _______ _ __ ___ _  ane 32                                    33  Specification of sensor   mmm 35  Parts layout m 36  How to replace UV lamp  mm 40  How to replace water level sensor  mm 42  TETTE ELEM  mE 43  Mounting procedure of        200        45  Honto stack      49    User s manual  m 51       ALARMS  amp  SAFETY FUNCTIONS               This unit has the alarms        safety functions shown in table below  and also self diagnostic functions     Alarms and safety functions  Alarm  amp  Safet Safety operation    If the chamber temperature exceeds   Over heat lamp lights        Upper limit or E12  E13  E14and chamber  temperature alarm ur upper limit alarm temperature set temperature are displayed Continuous tone Heater OFF  alue   alternately     if the chamber temperature deviates x      Automatic set 4 All digits on the Intermittent tone with  temperature alarm    set temperature by   1  C or temperature indicator blink    15 minutes delay  oom    Automatic set CO     the chamber CO  density deviates _   All digits on the CO  Intermittent tone with  density alarm from the set value by  1  or more  de
11.   9   212     F13    F21    Confirm the time of use for UV lamp  Display the voltage of CO  Sensor  Change the output ratio of bottom heater      Change the output ratio of door heater               Change the setting of Auto Changer  Demo mode  and Auto zero  mode  Display the temperature of CO  sensor box    Display room temperature sensor temperature       Initialize non volatile memory    214     Check LED and various driver software      Set communication device ID    F22     Set communication parameters    How to use When the internal chamber temperature is displayed  keep pressing    CAL key for more than five seconds  and then you will be in temperature    calibration mode  Then  by using      key           key  enter function    codes in the temperature display and push ENT key     FO1  Purpose  Change the time of turning UV lamp off  Preset time  5 minutes     Range 0 30 minutes      How to use  Enter    F01    in the temperature display and push ENT key  and    then you see the current setting value in the CO  display     By using                      key  change the setting value and push    ENT key  Then the value is recorded  and the display returns to    current temperature display      Action  UV lamp will be turned off automatically according to the setting    time after door closes  Please note that there will be three to five  seconds delay  since the sensor checks the water level of  humidifying pan upon closing the door  In addition  the UV lamp 15    off
12.   SM       A Oc    D or CNB                L      Fi        a a      DP     E 2   CN101       1                        EE       RED   amp         PINK      CO2 SENSOR                               i         ORANGE         YELLOW  R    02 27   c                       _    BL   BLUE      L  BL   LIGHT BLUE  9  TM        op             psa                       GN   GREEN  I W  WHITE        TIE      fu        PWB                          GY   GRAY  K  BR   BROWN     E   a        Qu dd E MTR 480   CO 20AIC        e     BK   BLACK  8  5   SHIELD  27              gt       gt                               DISPLAY    gt  ot                  lt      t                VIE  E    A    E                 x       gt   5 CIRCUIT DIAGRAM                       t diagram      Ircul         C                                                                                                                  die eaim oa               eed taf re ELLE          95  Cus        TEMP DiSPLA  Y  n2 DIAPLAY                                                                                                                                                                                         sn                                                      n P     M                                PPP                   Ape              d  x 11                       5 B  B 43193 Anu   5       wood                              QNEM                                                 _                                              
13.   display shows the input voltage of CO  sensor  Pushing  ENT key  the Auto Zero will be forcibly executed within one minute   Since the 90 seconds Auto Return is invalid with this mode  push    ENT key and end the mode     FO6  Purpose  Adjust humidity level by changing the electricity output ratio at  the bottom heater  under humidifying pan     Range  0 19  Initial value         How to use  Enter    F06    in the temperature display and push ENT key  and  then CO  display shows a current temperature  When you want to  raise humidity  increase the value in the CO  display  When you want  to reduce humidity  decrease the value in the CO  display  After  entering values  pushing ENT key let you return to current    temperature display mode     207                    Prevent dew condensation by changing the electricity output  ratio at door heater      Range O   9  Initial value  4      How to use   Enter  F07  in the temperature display and push ENT key   and then CO  display shows current setting values  If the door has  dew condensation  increase the value in the CO  display  Then  upon  pushing ENT key  it memorizes the values and returns to current    temperature display mode     208   Purpose    Change the setting for Auto zero function  Auto changer  on and off function  and demonstration mode       Auto changer mode is used when CO  needs to be supplied with two CO   cylinders  With the  Auto changer mode   the lamp of cylinders  currently used among A B lamps at displ
14.  5  or more  pure    Install a pressure regulator on the cylinder  Use a regulator rated at 25       on the primary side and  0 2MPa on the secondary side  Use the following procedure to attach the incubator to the cylinder     1  Using the gas supply pipe provided  connect the pressure regulator to the CO  inlet located at the rear  left hand side of the        incubator     2  Set the        pressure on the secondary side to 0 03          0 3kgf cm G  4 3psiG   Excessive pressure  may cause disconnection of internal pipes inside the        incubator which will result in leakage of        gas  into the atmosphere  Elevated level of CO  gas can be hazardous to health and may lead to  asphyxiation and risk of death     3  Check that no gas is leaking at any point where the pipe connects with the CO   regulator or the CO   incubator     Note   The incubator  including the gas supply pipes and services must be examined at frequent intervals to  ensure they are safe  Ensure that items such as pipes are replaced if there is any sign of deterioration                                                              Automatic switcher of CO  gas supply line    An automatic switcher of CO  gas supply line         20      is available as an optional accessory  This kit  switches the gas supply line when one CO   gas cylinder is empty        Note  The installation of MCO 20GC should be implemented by a qualified service personnel   After attachment of MCO 20GC  do the following   1  Connect
15.  Auto changer mode  switch the cylinder in use to  another one  Cylinder in current use is indicated by the lamp either A or B  which is lighting    When the cylinder in use is empty  automatically switches to another one  The  lamp of empty cylinder will blink and the lamp of another cylinder will turn on    Ex  When the current        cylinder    is empty  the lamp of cylinder A lighting  switches to blinking  and then the lamp of cylinder B turn on     zu      Temperature control  Setting range  0C     50      Display range  0  C  99 9  C    Setting method  Press the SET key  Change the value to the desired value with  2  key  and     key  then press the ENT key  The changed set value will be  memorized and change to        setting mode     Out of setting range  if the set value is changed out of setting range and pressed the SET  key  buzzer will sound one second continuous sound   set mode remains     Control  PID control method    Alarm  When the internal temperature is more than    1  C of set value       CO   Control  Setting range  0    209    Display range  096 99 996    Setting method  Press SET key twice  Change the value to the desired value with            and    key  then press the ENT key to memorize the set value  Then the  display will change to check mode for overheat protection temperature     Out of setting range  If the SV is changed out of setting range and pressed the ENT key   buzzer will sound one second continuous sound          density set mode  re
16.  B ard inside back cover   It shows about 4 C higher than    actual temperature        13                  Initialize nonvolatile memory  This function should be used only when nonvolatile memory data 15  destroyed and can not be recovered due to unforeseeable reasons  such as noises  Therefore  it SHOULD NOT be used without good  reasons     How to use  Enter  F13  in the temperature display and push ENT key  and  then CO  display shows  000   Then  when you change the first figure  to    1    and push ENT key  all the data of nonvolatile memory will be  initialized     The initial set values of nonvolatile memory are as follows     Inside temperature  37         CO  density   O  0    Balance parameter for bottom heater      Balance parameter for door heater 4   Zero Adjustment data for inside temperature  O   Zero Adjustment data for CO  density   5000  CO  span data   5000  Key lock data   Key lock OFF  Auto changer data   Auto change OFF  Demonstration mode data   Demonstration mode OFF  Auto Zero   Auto Zero         F14  Purpose  Check LED and various driver software    How to use  Enter  F14  and push ENT key  and then the lamp and display  blink every 0 5 seconds  Push ENT key to finish checking  or  when    you leave it for approximately 90 seconds  it automatically stops     F21  Purpose  Set ID for serial communication   How to use  Enter  F21  in the temperature display and push ENT key  and  then CO  density display shows  000   Then  entering values to  distingui
17.  Castor with adjustor  roller base  MCO 20RB    2  Automatic gas switcher  assembled in unit  MCO 20GC    3  Communication Board         480    4  Extra stainless steel shelves  1 shelf   1 shelf support basis  MCO 58ST    5         pressure regulator  MCO 100L     Note   Model name    M CO   20 A              C  Copper alloy anti bacteria stainless steel      Infrared       sensor   A  Air jacket   20  Chamber capacity  200L    CO       Incubator          M  Medical equipment         Structural specification _ iil           incubator  air jacket type   Zinc galvanized steel with polyester paint  Bio gray   Antibacterial stainless steel  containing copper   ball corners    Polymer alloy  Modified polyvinyl chloride   Tempered glass   thickness 5mm      pcs   W 580 x D 450 x    12mm    Tray maximum 15pcs  available   Maximum load 5kg 7 tray                  Shelf support x 5 set  Humidity pan x 1  otacking plate 1set  Tube fixing    2  Tube x 1       Antibacterial stainless used parts   O Inner cabinet  Tray  Tray support  oide support  Top duct  Rear duct  Humidifying pan cover                           humidity pan is not made of antibacterial stainless          Performance specification          and humidity range Humidity  less than 80  RH  in the unit  Setting range  0 50       in the unit    Variation range of temperature 0 71     in the unit  Temperature recovery period 25 minutes recovery to 36 5  C door open for 30 seconds         density range 0  2096         density
18.  When internal        density is 1  out of setting value  all figures of        density  display brink and buzzer sounds after 15 minutes later     6  Key lock function   Lock               PV display  keep pressing  2  key  shift key  over 5 seconds to enter the  key lock mode  display will change    L 0    When pressed      key and set at 1   Key lock ON   When pressed      key and set at 0   Key lock OFF   Then pressed SET to memorize the setting and return to PV display     Note  It can be entered the set mode with key lock on  but other mode can not be   entered     7  Door alarm  Display  When door open          Door check lamp illuminates   When door is closed   Door check lamp does not light on     Safety operation  When the door is open  fan motor will turn off         valve will be shut off   If the door is open over 60 minutes continuously  heater will turn off     8  Auto return  Function  During setting mode  key lock mode and function mode  if there is no key  operation over 90 seconds  it will return from setting mode to PV display  automatically     9  Calibration  Refer to page 9 and 10         15        10  Function Mode    FOO   FO1   FO2     The display returns to temperature display   i e  EXIT   Change the time of turning UV lamp offs  For general users    Change the setting of UV lamp 24 hours sterilization    FOGS Enter service code code  384         Will be used by a service    person  This will be the same up to   14     FO4   FOS   FOG     FO7      
19.  a       gas pipe to port A and B respectively     connecting port for CO   gas pipe is located on    the left back of the unit   See page 9      2  Connect          gas oylinder provided with a gas pressure regulator to each gas pipe  See page 14  for  the connection of the gas cylinder     3  Open the valve of each gas cylinder    4  Check that the        gas supply line indicator on the control panel is lit by pressing the switching key    5  Select          gas supply line     or B     6  When one cylinder is empty  the indicator is flashed  buzzer sounds  and      01    and current chamber  temperature are displayed alternately on the control panel while the gas supply line is switched to other one   To silence the buzzer  press the buzzer stop key  BUZZER     7  Replace the empty CO   gas cylinder    Note  Exercise caution when handling empty        gas cylinders as some gas can be still be left in the    cylinder     This kit MCO 20GC detects that no more        gas exists in a cylinder when the        density in the chamber  is not increased after a few minutes of opening of        gas valve in the unit and switches the gas supply    line         switching of supply line can be caused by some other reasons  blocking or restricting of gas  tube  reduction of CO  gas pressure  or improper opening of CO   gas cylinder  in spite of gas quantity in  the cylinder  Therefore  always check the gas quantity in the cylinder prior to disconnection          67                OPER
20.  control range   0 15   CO   density recovery period 10 minutes recovery to 5 096 door open for 30 seconds  Humidity in the unit 95 5  RH  Humidity recovery period 30 minutes recovery to 90  RH door open for 30 seconds  Supply CO   gas pressure 0 03MpaG  0 3kgf c m   Adjust with gas flowing   Power supply Single phase  Local voltage  Total power consumption 380W  Quantity of radiation        1370kJ h    Testing conditions  Temperature in the unit 37  C         density set 5 0   No loading  Ambient temperature 20  C  Ambient humidity about 45             Electrica  specification        Heating method Heater jacket   air jacket   Temperature control            2  Sensor  Thermister              10          Detect and control the inside temperature directly    Temperature indication   Digital display  resolution  0 1               density measuring          density indication Digital display  resolution  0 1          Natural evaporation      humidifying pan         REUS Humidifying pan  W293 x D407 x H4imm   Automatic ON   OFF control  changeable with Function mode    Working with door switch  UV lamp turns      for 5minutes after    every door close    OFF mode  UV lamp does not turn on     UV lamp control       Inside air circulation Breeze vertical air flow  turbo fan fan motor     Both display for temperature and CO   density are flashing  and  buzzer sounds intermittently after 15 min         Independent alarm for overheating   Door check lamp        Independent thermistor 
21.  for each heater is open circuit   E17  Sample pump and Auto zero pump is abnormal     Error code details are follows     E01 CO   tank is empty  When internal        density is not increased to 0 296 for 1 minute with                  opened  it displays    E01    alternately with  Internal temperature        temperature display and buzzer sounds  at intervals   It repeats twice for  definite action that        tank is empty  therefore it takes about 2 minutes to  display error after the tank was empty     E05 06 Internal temperature control sensor is abnormal  open   short circuit    When internal temperature read by micro computer is lower than 0      it regards as   open circuit   displaying    E05    alternately with internal temperature and sounding  buzzer  at intervals   When internal temperature read by micro computer is higher  than 60  C  it regards as    short circuit    displaying    E06    alternately with internal  temperature and buzzer sounds  at intervals   At the same time heater is OFF     Remark  At the status of E05    206  internal temperature displaying is different  from products  internal temperature      E07 08 Sensor of        sensor box is abnormal  open   short circuit   Refer to E05 06    Remark  At the status of E07  amp  E08  CO  valve is CLOSE      E09 10 Ambient temperature sensor is abnormal  open   short circuit   Refer to E05 06         13        E11 CO   sensor output voltage is abnormal   It regards        sensor is abnormal  when tempe
22.  key lock is set to ON   ula bi The current chamber temperature is displayed         Note     The key lock function is available for temperature and        density setting   To cancel the key lock  set to LO in the above procedure              70        OPERATING INSTRUCTIONS       Water level sensor          This unit is provided with a water level sensor for the humidifying pan  The sensor is set in active position  with respect to the installation of the humidifying pan  Take care not to damage the sensor at the time of  removal or installation of the humidifying pan     When the humidifying pan is removed  side view     When the humidifying pan is installed  side view     Sensor    IA 3    Note  At the installation  check the humidifying pan is located far depth and the sensor is down        Note      Lift the sensor before installing the humidifying pan if the sensor is in lower position after maintenance      Use an alcohol for sterilization when cleaning the sensor  taking care not to stress the lead wire      The sensor detects the water level every 30 minutes  In addition  detection is executed after operation of  the outer door  It takes several seconds to detect the water level  Therefore  the water level alarm lamp  may flash a few times after the outer door is closed with the humidifying pan filled sufficiently         11          Contents    Page   Ead     eee 1   Structural specification        2   Performance specification       n 3   Electrical specification 
23.  rain water     Only qualified engineers or service personnel should install the unit  The installation by  unqualified personnel may cause electric shock or fire     Install the unit on a sturdy floor     the floor is not strong enough or the installation site is not  adequate  this may result in injury from the unit falling or tipping over     Never install the unit in a humid place or a place where it is likely to be splashed by water   Deterioration of the insulation may result which could cause current leakage or electric shock     S Never install the unit in a flammable or volatile location  This may cause explosion or fire     Never install the unit where acid or corrosive gases are present as current leakage or electric  shock may result due to corrosion     9 Use a dedicated power source as indicated on the rating label attached to the unit     9 Remove dust from the power supply plug before inserting in a power source     dusty plug       improper insertion may pose a hazard     o Use a power supply outlet with ground  earth  to prevent electric shock  If the power supply outlet  is not grounded  earthed   it will be necessary to install a ground by qualified engineers     S Never ground the unit through a gas pipe  water main  telephone line or lightning rod  Such  grounding may cause electric shock in the case of an incomplete circuit     Check the gas type and ensure that it is fit for the purpose  Make sure that all pipes are  connected correctly and are not lia
24.  while the door is open     202                   Change the setting of UV lamp 24 hours sterilization mode    How to use  Enter  F02  in the temperature display and push ENT key  and  then CO  display shows  000   By using 2 key  enter  001  and  push ENT key  Then  current temperatures will be displayed and  24 hours sterilization mode will be turned on    If leaving  000  on  push ENT key  Then  the display will return to  the current temperature display mode     Action  When      02    set 24 hours sterilization mode  UV lamp will be turned  on for 24 hours  then off automatically  However  when you open  the door  24 hours sterilization mode will be canceled    Also  if 24 hours sterilization mode is set with    F02    again while  24 hours mode is already set  24 hours sterilization mode will    begin at the moment     FO3  Purpose  Note that those who knows service code should use the functions  below    How to use   Enter the following code to use the functions below   Code 384   Enter              in the temperature display and push ENT key  and then  CO  display shows  000   Next  by using 2                  key  enter  the service code   384  and push ENT key  The display will return to  current temperature  and you will be able to use the functions  following   04   If you pushed ENT key  for example  to use  F05  without entering  the service code above  continuous sound will be going off for one  second  and the display will keep showing    F05     In this case 
25.  you can  use F05 function by entering the service code above for               function   Note  It will memorize the service code above  unless you enter  000  for     F03  function or turn off the mains switch     FO4  Purpose  Display the time of using UV lamp    How to use  Enter      04    in the temperature display and push ENT key   Then  CO  display shows the time of using UV lamp by percentage    It considers as 1  1000 hours for lifetime of UV lamp   Display shows addition time by percentage up to that time     How to add time of using UV lamp     1  5 minutes  for setting time of using UV lamp is less than 5 minutes   2  Using minutes  for setting time of using UV lamp is 6 30 minutes   3  24 hours  for just after pressed ENT  001  at F02 when 24 hours   sterilization mode was activated  This additional time is memorized  on nonvolatile memory once in 24 hours     Remarks  When 24 hours sterilization mode is activated  add 24 hours  without considering cancellation of mode that is caused by  open door  and so on       How to clear addition time is to display addition time at F4  then press  ENT by using      key             key to make CO  display    000          The range of addition time percentage is 072609  not displayed when it is  more than 260      FO5  Purpose  Display the input voltage   A D converter input voltage inside the  microcomputer on main board  of CO  sensor    How to use  Enter    F05    in the temperature display and push ENT key  and  then CO
26. 03AT 1 103AT 1 103AT 1   Rating 10KQ  25  C  10KQ  25  C  10KQ  25  C            Temp  control sensor         lt              Over heat protect                2                  GL4  AW    103AT 1 103AT 1 103AT 1    Type             c  2         Door switch Type         D  c  2                     20    2  5   gt                      solenoid                   lt   O                  FG 7P      230   60Hz 4W  Type    7    2210 115 ZHG2210 11S ZHG2210 11S       250V 10A  Type       BAM215131       BAM215131                215131   DC 5V power supply WRMOSSX 0U    WRMOSSX OU                         85   264VAC 85   264VAC 85   264VAC  Transformer for P C B   P C B     JJ JJ              5 3           Ballast    JU  iV                Noise filter           lt              Mains switch           lt              Air pump Type  Auto zero air pump   UV lamp   Glow starter       MCO 20AIC      ACT10 115V 60Hz AC220V 60Hz AC220V 240V 50Hz    Side heater Rating    Right  amp  Left    Frange heater Rating    Bottom side heater Rating    Right  amp  Left    Bottom heater Rating           sensor box heater    Rating           sensor box heater B Rating    Hinge sub heater Rating    Right  amp  Left    Front heater Rating    Bimetal thermostat                  Dd 60  C ON    70  C OFF  60 C ON  70 C OFF  60  C ON    70  C  OFF    Agitating fan motor             ______    2   01 1Y1M FL2   011Y5M FL2   011Y5M    Rating     110   115VAC 220   240VAC 220   240VAC               sF70U SF70U SF7
27. 0U  Rating    250       7   70     OFF  250VAC 7A 70  C OFF  250VAC 7A 70  C OFF    Rating    Thermal fuse Type            Temperature calibration       note  It is not possible to enter calibration mode with key lock on    1  Press key for about five seconds    2  The top  third  digit of temperature display is blinking  other digits will go off      3  Set the present correct temperature with         and      key  Press key    4  The display will automatically revert to the present temperature      Example  If the internal temperature display shows 37 0  C  the set value   the actual  measured value is 36 8         D Press key for about five seconds    2  The    3     top digit  of temperature display is blinking  other digits will go off    S Set the displayed value at 36 8  C of the actual measured value with        key  and   key  then press key    4  The display will automatically revert to the present temperature    Note           temperature calibration  it is important to accurately measure the internal temperature   Particularly  for the thermometer used  its grade of accuracy should be than JIS 0 5 class   The measure should be carried out at the center of the chamber               temperature calibration range is between the set value  1 0  C  If the value over this  range is input  an error tone will be emitted  the input data will be ignored  and the display  will revert to the present temperature  Therefore  if you need to calibrate more than 1 0       you shou
28. 16 10104      g    VRIOL      e              52  2        8 13         CN201 24    0102 o P106 P107  X UE   7 SET RTT  CAL BUZZER       TEX      1  103        72          17 TET 25  _ mt    gt  5   104 cour         a            5   _ 150 156           RIG 5 5  iy    3151       5104       4105 2 5  107  Of 5225232 5 o  F 5  5 js      48            gt  gt  5 SW0BA 2                    124 0106    CO 20AIC Serial NO   Ne        4   101 051 00 2  BOARD        CNI CN3 45 IN      IN  GNO      CNI4 CN302  GNO CNS mutus                     5  2    SOR    CO 20AIC  9 5     8                B41 4    5 BOB   7FB 4 8101 051 00  2 BOARD   ur          85  ze    88 5 Serial         2 4  our a 580        AW           53   1  21 8 saec         TP3                    aps           J5i        CIO       85 e  5                       Qe                                     HI icicle      3ev x        R7 J3e 4 5 450   18 R305       YR diu     5 t     AF us  ae            Le E     amp                     050       1 C4                Lit 0305 5    1 8  lt   E                                                          RA      Re      cas           rs    _     129   1 01 130     Vy       et 1       C8             IER   do 2     C32 d NS        8          46       hi  E          TP 48 8  5       RI       oes    TE        n   E                               18        9  mi   5          i 2  1        18              JI2 a  CC     z                CLOS          a w  7           lt       8  J13     NO  x    
29. ATING INSTRUCTIONS    Table below shows the basic procedure for setting the chamber temperature and CO  density  The upper  limit alarm temperature setting is also shown in the table  Perform key operations in the sequence  indicated in the table  The example in the table is based on the assumption that the desired temperature  is 37  C and        density is 5     Note  The unit is set at the factory so that the chamber temperature is 37  C and        control is 0         Allow at least 4 hours until the next setting after setting of desired chamber temperature and setting        density to 095  at the time of first start up or start up after no use for long term           Basic operation sequence  Example  Chamber temperature  372          density  5        Description of operation Indication after operation      The current chamber temperature is displayed  Turn the power switch ON      in temperature indicator   Press SET key  The left digit is flashed     3    By pressing        key and    key  set  o      When pressed  the changeable digit is shifted   the figure to 37 0  BEL   When pressed  the figure of settable digit increases     Press ENT key  Set temperature is memorized  31  4 ENT Left digit in        density indicator is  flashed  UL  5                c3                c3    8   gt   c2    By pressing      key            key  set  gt        When pressed  the changeable digit is shifted   the figure to 05 0              When pressed  the figure of settable digit i
30. Check that the lower unit is level     Stacking plate A         Stacking plate B    Fixing screws    Rear cover        49        6  Apply the protective sticker enclosed with the unit at each corner      the top of the lower unit to avoid  scratches or damage     T  Fix the stacking plate A at 2 locations on the top front of the lower unit by using 2 screws removed in step  3     8  Remove the front panel on the upper unit by unscrewing the 4 fixing screws and then disconnect the  wires and gas tube       9  Stack the unit so that both units can be aligned straight  Also check the upper unit is level  If it not  level  keep the unit even by adjusting the leveling legs     10  Secure the upper unit with the stacking plate A and 2 screws removed in step 3   11  Remove 2 hooks on the rear side of the lower unit by unscrewing each 2 fixing screws   12  Remove 1 screw on the bottom right and left on the rear side of the upper unit     13  Fix the stacking plate    at the right and left on the rear of the lower and upper unit with 3 screws   removed in step 11 and 12     14  Replace the front panel on the upper unit after connecting the wires and gas tube     15  Fix the stacked unit to the wall with 2 hooks on the rear of the upper unit and rope or chain     Front panel       Stacking plate B    Stacking plate A    Hook         Protective sticker      This section is extracted        printed from User s Manual           you find out  Refer      page    6  in them  this page means 
31. Contents    Page   Ead     eee 1   Structural specification        2   Performance specification       n 3   Electrical specification   4                                                         7   Temperature calibration        9         calibration      10  Control specification      M H   mw 11  Wiring diagram   nn 26  Circuit diagram      27  Silk Print on PCB  E                                                           28  Connection on PCB  rc xc     29  Tubing diagram  mms 31                     _______ _ __ ___ _  ane 32                                    33  Specification of sensor   mmm 35  Parts layout m 36  How to replace UV lamp  mm 40  How to replace water level sensor  mm 42  TETTE ELEM  mE 43  Mounting procedure of        200        45  Honto stack      49    User s manual  m 51    SANYO    Service Manual       Incubator    MCO 20AIC               2212          206 n    00200 00000    5  7210110       Effective models    This service manual is effective following models              name                                                           Contents    Page   Ead     eee 1   Structural specification        2   Performance specification       n 3   Electrical specification   4                                                         7   Temperature calibration        9         calibration      10  Control specification      M H   mw 11  Wiring diagram   nn 26  Circuit diagram      27  Silk Print on PCB  E                                                      
32. In        density indicator      0    is displayed and the    Press ENT key   ENT  right digit is flashed    10   By pressing        key and  amp  key     set only the left digit figure to 1   NOTE  Don t change center     means  0  or  1                digit figure and right digit  fiqure    Press ENT key  Setting of MCO 20GC is finished    The current chamber temperature is  displayed    CO   gas supply line indicator A is lighted            47        Procedure for automatic gas switching operation check   When the installation of MCO 20GC is completed  check the automatic gas switching operation  according to the following procedures     procedure      Stop      gas supply of cylinder    and use cylinder B only for        gas supply               on      power switch of MCO 20AIC and set 37 C and 0    Turn on CO   gas supply line indicator A with CO   gas supply line switching key   3   Wait for 1 hour approx   until the machine control the CO   density       It takes about 1 hour from turning on      power switch until        control is  enabled    Set        density 5  and check the        inject lamp A turns on     NOTE  In case of low ambient temperature  it may take more than 1 hour until the  machine can control CO   density and the CO   inject lamp turns on    Check the   01      gt  gas cylinder empty alarm  and buzzer turn on about  2 7               later from the CO   inject lamp s turning on    In this case the gas cylinder A lamp should be blinking  and the ga
33. NCAUTION    o Select a level and sturdy floor for installation  This precaution will prevent the unit from tipping   Improper installation may result in water spillage or injury from the unit tipping over        9 Connect the unit to a power source      indicated on the rating label attached to the unit  Use of  any other voltage or frequency other than that on the rating label may cause fire or electric shock     9 Fix the shelves securely  Incomplete installation may cause injury or damage     o When removing the plug from the power supply outlet  grip the power supply plug  not the cord   Pulling the cord may result in electric shock or fire by short circuit     Never damage or break the power supply plug or cord  Do not use the supply plug if its cord is  loose  This may cause fire or electric shock     S Do not touch any electrical parts such as the power supply plug or any switches with a wet hand   This may cause electric shock     Do not put a container with water or heavy articles on the unit  It may cause injury if the articles  fall  Current leakage or electric shock may result form deterioration of insulation by spilled water     Do not climb onto the unit and do not put articles on the unit  This may cause injury by tipping or  damage to the unit     S  Never lean or press on the glass  Intentional force may cause injury if the glass breaks     S Do not lean on the door  This may cause injury  current leakage  or electric shock if the unit tips over  or door beco
34. NT key   4  The unit automatically reverts to the display mode      Example    If the displayed chamber temperature is 37 0  C  set value  and the actual temperature is 36 8         1  Press the CAL key for about 5 seconds    2  The    3    on the temperature indicator flashes  and the CO  density indicator goes out    3  Adjust the set value to the actual value of 36 8  C with the PP key and 4 key  then press ENT key   4  The unit automatically reverts to the display mode     Note    It is important to accurately measure the temperature inside the unit when performing temperature  calibration  Particularly  the temperature gauge used must have an accuracy of 0 5 Class or better  The  temperature must be measured at several points    The temperature setting must not change by more than    1 0  C during calibration  If it exceeds this        error tone is emitted  the input data is ignored  and the unit reverts to the display mode  Consequently  if it  is necessary to change the temperature by more than 1 09    perform calibration in several stages over a  period of time            calibration    opan setting  opan setting should be done under stable condition of temperature  humidity  and       density   1  Press the CAL key for about 5 seconds       2  The third digit on the temperature indicator flashes  and the CO  density indicator goes out     3  Press the CAL key once again    4  The third digit on the       density indicator flashes  and the temperature indicator goes 
35. REMOTE ALARM  MAX DC30V 2A    Remote alarm terminal            3          ROUTINE MAINTENANCE     NWARNING   Always disconnect the power supply to the unit prior to any repair or maintenance of the unit in  order to prevent electric shock or iniury    Ensure you do not inhale or consume medication or aerosols from around the unit at the time of    maintenance  These may be harmful to your health      NCAUTION    Always put on dry gloves to protect hands at the time of maintenance  Failure to use gloves may  result in cuts or abrasions from any sharp edges or corners        Sterilizing of chamber and attachments    When the chamber of the unit is contaminated  the chamber and internal attachments should be cleaned  and sterilized as follows     Note    Take care not to damage the UV lamp or water level sensor at the time of removal or replacement of  attachments        Do not clean the inside of the unit with a solution of disodium chlorate or other halogen based solution  because this may cause rust     1  Take out all trays and the humidifying pan from the chamber     2  Remove top panel  side support  right and left side   tray supports  and the fan as shown in the figures  on page 23 and 24     3  Clean all the attachments with neutral detergent and then rinse away the detergent with distilled water   4  Wipe the attachments with a gauze containing alcohol for sterilization and then wipe off with a dry gauze     5  Wipe the inside wall of the with a gauze containing alcoho
36. areful for the length of lead wire for water    level sensor inside the unit Length is connect  2  binder is located where it touches the  this V cut Sensor grommet     refer to Fig 4    If there is a V cut for water level installation  grommet  pls secure a space for this V  cut  Refer to Fig 3    3 Put the rear cover in place        direction of water  leVel Sensor Fig 3 Front view Fig 4        42                      to reverse the door       Inner door        1 Unplug the unit and make sure that power is not  supplied  2  lake the inner  and outer door off     1  Take out power panel which 15 at the front side  4  screws     2  Pull out the inner door hinge        2         take the  inner door off     3  Remove 2 part of the connector door lead wire    earth wire and nylon clip  Then remove the door lead  wire exit bushing and take the lead wire out     4  Remove 4pcs of outer door hinge installation screw  and take off the outer door           Be careful not to lose the attachment plate  for outer door hinge     Outer door hinge  installation scre    Door lead wire  exit bushing    3 Preparation to reverse the door it self     1  Remove the cover of outer door bottom part  and put  out the lead wire to the opposite side  and put the  cover back on     2  Place the hinge and screws of outer door to the  opposite side  Also at this time reverse the    hinge shaft upside down     Outer door hinge  shaft       Cover for outer door  bottom part    Door lead wire        43   
37. ay Board will be turned on   and then you will be able to change the cylinder in use by hand  Also   when the cylinder currently      use is empty    automatically  switches to another cylinder  and the lamp for empty cylinder will  blink     The demonstration mode is a function for exhibitions  With demonstration  mode on  it displays the set value of temperature and CO   which  makes it seemingly stable       the meantime  the electricity for  heater does not conduct  and CO  valve is closed       Auto zero function is for automatically zero adjusting CO  sensor based on   room air         Auto zero function is executed every 10 minutes  within one hour after turning on power and every four hours    afterward      How to use            Auto changer mode   Enter    F08    in the temperature display and push ENT key  and then  the third figures of CO  display shows current setting value  When  the value is    0    normal mode is on  Auto changer is off   When it is     17  Auto changer is on    Set demonstration mode   Enter    F08    in the temperature display and push ENT key  and then    the second figures at CO  display shows current value  When the    value is    0    normal mode is       Auto zero is on   When it is  1    demonstration mode is on  When you want to change  change the  value at second figure and push ENT key  and then it returns to    current temperature display      Set Auto zero mode     Enter    F08    in the temperature display and push ENT key  and t
38. ay PCB CN101      1      2 CO valve   3    4  CO valve  Option  CO   valve ON   OFF control   CN10 Hemote alarm terminal  he terminals make close circuit   1         2 N O  under the alarm condition   CN11  MTR 480  Option    1    2 Auto zero air pump   CN12    3    4 Air pump AC36V output   Hinge sub heater   UV lamp relay         81 82 close for UV lamp       ud       Or   CN15   Water level sensor   CN101   Main control PCB CN8     CN1  CN2  CN4  CN6  CN7  CN8     9              29                       10           1   Inner chamber heating up  7pcs   CN302    3 Bottom heater Heater control   5             heater and Door heater    CN303             Heater        fan motor control    3  Agitating fan motor           30             Tubing diagram        25      Capillary tube       sensor                                      Filter         CO   gas inlet Sample                     Prevention of contamination        In servicing for CO   incubator  it is important to prevent from contamination      1  More than 90  of contamination in        incubator is caused by mold  Mold is    kind of  true fungi  it has high increasing power under high temperature and high humidity   Accordingly  it is easy for mold to increase inside        incubator  Those increased mold  eat up culture  mainly animal cell   eventually precious test sample will be killed  Cell that  is independent from ecosystem is also independent from immune system  it has no  immunocompetence at all      2  Precau
39. ble to become disconnected  Ensure that the gas pressure  is set at the specified value  Improper connection of the gas pipe or use of incorrect gas  pressure may result in leakage of       gas  Elevated level of CO  gas        be hazardous to    health and may lead to asphyxiation and risk of death     S Do not insert metal objects such as a pin or a wire into any vent  gap or any outlet for inner air  circulation  This may cause electric shock or injury by accidental contact with moving parts     S Never store volatile or flammable substances in this unit  This may cause explosion or fire     PRECAUTIONS FOR SAFE OPERATION   NWARNING    As with any equipment that uses CO  gas  there is a likelihood of oxygen depletion in the  vicinity of the equipment     is important that you assess the work site to ensure there is  suitable and sufficient ventilation  If restricted ventilation is suspected  then other methods of  ensuring a safe environment must be considered  These may include atmosphere monitoring  and warning devices           Ventilate a room air occasionally when using CO  gas for control  The gas density will increase  in an enclosed small room and high level of gas density can be hazardous to health  n addition  avoid  inhaling the chamber air directly when opening the door if CO   gas is used    Si l appareil est utilis   dans un evdroit restreint  le niveau      la densit         de l air peut s   lever  et peut        nocif aux humains    vitez  d aspirer l air 
40. buzzer  at  intervals      E16 SSH for each heater is open circuit  When 55  for main heater  bottom heater  door heater and        sensor box  heater is open circuit  it displays  E16  alternately with  internal temperature  and  buzzer sounds  at intervals      E17 Sample pump or Auto zero pump is abnormal   When        density setting value is more than 3 5  and current density is more  than 3 5   and when voltage difference  during Auto zero  and  before Auto  zero  is less than 150mV  it regards that Sample pump and Auto zero pump is  abnormal    displays  E17  alternately with  internal temperature  and buzzer  sounds  at intervals   At the same time       valve is CLOSE  Self diagnosis is  done whenever Auto zero adjustment would be done  however it is ignored at  first short intervals of Auto zero         14        Alarm for internal temperature is upper limit  When internal temperature is over upper limit  the upper limit temperature alarm  lump is turned on and it displays  current internal temperature  alternately with   E12  main heater is abnormal   or    E16 SSR for each heaters is open circuit    and buzzer sounds  at intervals   It is impossible to stop buzzing by key  At the  same time main heater  bottom heater and door heater is OFF     Alarm for internal temperature auto set  When internal temperature is   1 0      out of setting value  all figures of  temperature display brink and buzzer sounds after 15 minutes later     Alarm for        density auto set 
41. e  where  incubator is installed   Exchange of room air should be done at narrow space    Please request to your customers         10          Contro  specification        1  Key        switch    BZ    SET                        CAL               When alarm LED is blinking and buzzer does not sound     Buzzer and remote alarm output       Force to turn on     When alarm LED is blinking and buzzer sounds   Buzzer and remote alarm output esee Force to turn off     However  when door alarm is activated  remote alarm remains OFF       Press once to change to setting mode    Press twice to change to CO   density setting mode    Press triple to change to check mode of over heat protection temperature   Press four times to change to internal temperature display mode       During setting mode  shift between the first digit and second digit and the first  decimal place     In PV display  keep pressing this key over 5 seconds to enter the key lock  mode       During setting mode  the blinking digit counts up      During setting mode  CAL mode and Function mode  press to memory the  value             PV display  keep pressing this key over 5 seconds to enter the temperature  calibration mode  press again to enter the       calibration mode    In temperature calibration mode  input FXX and then press ENT key to enter  function mode       Available during Auto changer mode  function mode F8  is set      No response and no light on lamp of cylinder in ordinary use    When press this key at
42. e supports        be removed for disinfection  See page 23 and 24   7  Top duct  Inlet of circulating air  Removable    8  Rear duct  Flow path for circulating air  Removable    9  Fan  inside the rear duct   Made from polypropylene resin         be disinfected by an autoclave   10  Sample air outlet  This also functions as an internal gas outlet  so do not cap it     11  Connecting port for CO  gas pipe  rear side             an optional component MCO 20GC  cylinder  changeover accessory  is installed  both    and B are available       MCO 20GC is not used  only    is  available  Refer to page 14 for gas cylinder connection  Ensure that the gas pressure is set at  0 03MPaG  0 3kgf cm G  4 3psiG      12  Door switch  Detects the door opening closing and stops the circulating fan and electromagnetic  valve for        when door is open  UV lamp is also deactivated by door opening     13  Humidifying pan  Use sterile distilled water to fill the pan  Install the pan properly so that it        be  covered with the pan cover     14  Humidifying pan cover  Prevents UV light being exposed to the chamber  When filling the pan  lift  the front side and take out the         See page 23 for details     15  UV lamp  Sanyo UV lamp does not generate ozone  Never look at the UV light directly          replacement  contact Sanyo representative or agent     16  Water level sensor for humidifying pan  Located at the rear of the chamber and detects the water  level in the humidifying pan  Never 
43. en low temperature water is poured  the chamber temperature drops significantly  Clean the pan once  a month     9  Humidifying pan and pan cover   The humidifying pan and pan cover prevent the UV light from escaping  Make sure they are installed  even if you do not need humidity  Install the pan cover correctly since it affects the temperature  distribution and humidity recovery in the chamber     CAUTIONS FOR USAGE    10  Stored materials    Never place acid or alkaline materials or materials that release corrosive gas in the chamber  Such       materials can cause failure resulting from discoloration or corrosion     11  Alarm  Always investigate the cause and fix the alarm condition immediately when the alarm is activated  Refer  to page 20 for alarm details     12  Never use CAL key   Never use CAL key during normal operation because the calibration mode is started by pressing this key   Wrong operation of this key will give an adverse effect      the basic performance of the unit  If you press  this key accidentally  do not press any keys on the control panel for 90 seconds  after which the unit returns  to the display mode automatically     13  UV lamp   The UV lamp is OFF when the outer door is opened as it is operated to outer door switch  Check the UV  lamp is ON with the inner door closed once a month by activating the outer door switch     the lamp is not  ON  the lamp needs to be replaced  Contact Sanyo sales representative or agent    The UV lamp is located in t
44. ent will be carried out every 4 hours   Refer to the timing chart  below       1  Mechanism of auto zero adjustment   oRun the auto zero pump for two minutes to feed ambient air into        sensor  During  this period  sampling air pump is off         density in the atmosphere is almost 0    0 03967 0 0796   adjust automatically that the measured        is 0               do usual sampling for two minutes  compare CO  density of the ambient air with  the inside of the chamber  At this time  only air pump for sampling from the chamber  is running      2  Timing chart of auto zero adjustment    Auto zero          Sensor box    4min    4min    4min    4min    4min    4min    4min    Warmup                       10min  passed  20min  passed  30min  passed  40min  passed  50min  passed     30   60min    10        10min  10min  10min  10min  4hours 4hours     3  Indication of auto zero adjustment  During auto zero adjustment  indicator of        density is locked as the density  which 15  immediate before the auto zero adjustment  is displayed and the decimal point of the  indicator is flashing  It is changeable the set value is of        density during auto zero    adjustment  4            indication       Flashing     NOTE  If        density is set at 0         indication shows nothing  so the decimal point  also couldn t be seen      4  Auto zero adjusting method  Auto zero adjustment is carried out by adding a quarter of difference between each  measurement so as not to change 
45. er unit by unscrewing the 4 fixing screws and then disconnect the  wires and gas tube       9  Stack the unit so that both units can be aligned straight  Also check the upper unit is level  If it is not  level  Keep the unit even by adjusting the leveling legs     10  Secure the upper unit with the stacking plate A and 2 screws removed in step 3   11  Remove 2 hooks on the rear side of the lower unit by unscrewing each 2 fixing screws   12  Remove 1 screw on the bottom right and left on the rear side of the upper unit     13  Fix the stacking plate B at the right and left on the rear of the lower and upper unit with 3 screws  removed in step 11 and 12     14  Replace the front panel on the upper unit after connecting the wires and gas tube     15  Fix the stacked unit to the wall with 2 hooks on the rear of the upper unit and rope or chain     Front panel    Stacking plate B       Stacking plate A    Hook         Protective sticker    SPECIFICATIONS           Incubator  MCO 20AIC  22 W770    D708 x H900  mm   W620 x D523x H665  mm     Name      Polyester finish baked on zinc galvanized steel  Stainless steel containing copper    Tempered glass  Tray 5 trays made of stainless steel containing copper     W580 x D450 x H12  mm   Maximum load  5             Inner diameter  30 mm  On the back side  Rigid polyurethane foamed in place  CFC FREE          system  heater jacket   air jacket system     Access port    Heater       Lis  gt  5 1911115 Im  c o                              g
46. gasket of the inner door completely sealed    4  15 the access port at the upper left corner opened     If normal cultivation cannot be done and chamber gas density is suspect  1       the environment around the unit normal  Is the source of the contaminated gas nearby   2  15 the unit installed in an enclosed space     If it takes much time to recover the gas density   1  HEPA filter is provided in the gas piping  If it takes much time to recover the gas density even though  the gas pressure is normal  it may be that dust on the HEPA filter prevents the gas flow  Consult the  Sanyo dealer or agent         19             ENVIRONMENTAL CONDITIONS    This equipment is designed to be safe under the following conditions           1  Indoor use     2  Altitude up to 2000 m   3  Ambient temperature 5 C to 35 C    4  Maximum relative humidity 80  for temperature up to 31     decreasing linearly to 5096 relative humidity  at 40         5  Mains supply voltage fluctuations not to exceed  10  of the nominal voltage   6  Other supply voltage fluctuations as stated by the manufacturer     T  Transient overvoltages according to Installation Categories  Overvoltage Categories        For mains  supply the minimum and normal category is II     8  Pollution degree 2 in accordance with IEC 664     DISPOSAL OF UNIT      WARNING    If the unit is to be stored unused in an unsupervised area for an extended period ensure that children do       not have access and doors cannot be closed completely 
47. he duct  Take care not to damage the lamp at the time of installation removal  of attachments or humidifying pan    Note  UV light is harmful to the eyes  Never turn on the UV lamp with the inner door opened     14  Notice of recommended replacement of UV lamp   This unit is provided with a function to notify the recommendation of UV lamp replacement when the  accumulated ON time of UV lamp is over about 1 000 hours  The blink of the UV indicator on the control  panel recommends the replacement of UV lamp  For the replacement  contact Sanyo sales  representative or agent     15  If not used for a long period   When the unit is not used for a long period  dispose of the water in the humidifying pan and completely  remove any moisture in the chamber completely             that the chamber is completely dry before  closing the doors                                          5                                               5    1  Outer door  Sticks to frame with magnetic seal  Door heater is installed in the door panel  The  heater eliminates moisture on the inner door  The door opening is reversible  Contact Sanyo  representative or agent to change the door hinge from left to right or vice versa     2  inner door  Made of tempered glass  Avoid excessive impact on the glass    3  Leveling foot  Used for leveling the unit    4  Tray         be pulled toward you    5  Tray support  Can be removed by lifting the front side and pulling toward you    6  Side support  Right and left sid
48. hen  the first figures at CO  display shows current value  When the  parameter is           normal mode is on  When it is  1   Auto zero mode  is off  When you want to change  change the value at first figure and    push ENT key  and then it returns to current temperature display     FO9  Purpose  Display the temperature inside CO  sensor box     How to use   Enter    F09    in the temperature display and push ENT key     and then CO  display shows the current temperature inside CO   sensor box  When you push ENT key or leave it for 90 seconds  it  returns to current temperature display mode              preset temperature is 45   C  When it fluctuates more than    2  C from the preset temperature  CO  valve will be closed  When it  returns to within  2  C from the set temperature  it automatically  executes Auto Zero  adjustment      When CO  does not inject  the temperature of sensor box may not  be within  2  O  C  or Auto zero may be being executed  or it may    be within 60 minutes after power is turned on     F12  Purpose Display the temperature of room temperature sensor     How to use  Enter    F12    in the temperature display and push ENT key  and    Note     then CO  display shows the temperature of room temperature  sensor  When you push ENT key or leave it for 90 seconds  it    returns to current temperature display mode            room temperature sensor may not show correct room    temperature due to the heat from electrical parts  since it is located    on P C
49. icator       0    is displayed and       right digit is flashed    By pressing        key and 4            set the figure to O00     11   Press ENT key  Setting of MCO 20GC is finished   ENT The current chamber temperature is  displayed     When checking the UV lamp operation  open the outer door and push the door switch with the       inner door closed     The recommended timing of lamp replacement  the ratio of UV output is less than 70  of initiai  value  is when the accumulated ON time is over 1 000 hours  The blink of the UV indicator    when the UV lamp is OFF means the accumulated time has exceeded 1 000 hours ana    recommends the replacement of the lamp        E                     to replace water level sensor         1 Unplug the unit and check that power is not supplied to       the unit  then take the rear cover off   2 Unfasten the connectors for lead wire and take out the  insulation for the lead wire hole     Water level sensor  lead wire connector    Insulation for             lead wire hole    3  Take out the duct from inside    4 Unfasten the 2 screws to remove unit of water level  sensor and installation grommets for water level  sensor    5 replace water level sensor   Be sure to set the water level sensor to the   right direction    6 Place back the water level sensor  Water sensor   installation grommet as before     Water level sensor    7 Place back the insulation material to the hole for the Installation grommet    lead wire  Water level sensor  Be c
50. l for sterilization and then wipe off with a dry  gauze       6  Replace all attachments in the chamber   Note     Always insert the fan on the motor shaft surely                   insertion may cause poor performance     T  Fill the humidifying pan with sterile distilled water            TA    ROUTINE MAINTENANCE       Removal of attachments    Note   Take care not to damage the UV lamp or water level sensor at the time of removal or replacement of  attachments       1  Close the valve of the gas cylinder and turn off the power    2  Open the outer door  Remove 2 pins fixing the inner hinge and remove the inner door    3  Pull out the all trays  See Fig  1   4  Remove all tray supports on the right and left side by lifting up the front side  See Fig  2    5  By lifting up the humidifying pan cover  unhook it at the rear and remove the cover as shown in Fig  3     6  Take out the humidifying pan         Fig  4           1 5             ROUTINE MAINTENANCE               7  After removing 2 screws fixing the       duct  take out the top duct by removing       See Fig  5   8  To remove the rear duct  lift it up as shown in Fig  6    9  To removing the circulating fan  pull out the spring and then pull the fan  See Fig  7    10  Loosen and remove the screw fixing the clamp and remove the clamp         Fig  8 and Fig  9     11  By lifting the side support  take it off from the hooks  See Fig  10                  12  Replace all attachments      the chamber with the reversed order 
51. lacement of    attachments   Do not clean the inside of the unit with a solution of disodium chlorate or other halogen based solution  because this may cause corrosion of metal surfaces     1  Take out all attachment such as trays  humidifying pans  etc  from the chamber  Refer to page 23 and  24     2  Clean all of the attachments with neutral detergent and then wash out the detergent with distilled water   3  Wipe the attachments with a gauze containing alcohol for sterilization and then wipe off with a dry gauze     4  Wipe the inside wall of the chamber with a gauze containing alcohol for sterilization and then wipe off    with a dry gauze   5  Replace all attachments in the chamber   Note     Always insert the fan on the motor shaft surely  Improper insertion may cause poor performance     6  Fill the humidifying pan with sterile distilled water         65                BEFORE COMMENCING OPERATION       Connection of       gas cylinder     NWARNING  Check the gas type and ensure that it is fit for the purpose  Make sure that all pipes are connected  correctly and are not liable to become disconnected  Ensure that the gas pressure is set at the    specified value  Improper connection of the gas pipe or use of incorrect gas pressure may result    in leakage of       gas  Elevated level of CO  gas        be hazardous to health and may lead to  asphyxiation and risk of death        Use a liquefied        gas cylinder  not    siphon  dip tube  type  The CO   gas should be 99
52. ld repeat the calibration in several time          CO2 Calibration  1      1  Press key for about five seconds    2  The top  third  digit of temperature display is blinking  other digits will go off      3  Press key again      4  The top  third  digit of        density display is blinking  other digit will go off      5  Set the present correct       density with       key and 2                              6  The display will automatically revert to the present temperature    Example  If the internal        density display shows 5 0  the set value   the actual  measured value is 4 5      D Press key for about five seconds    2 The    3     top digit  of temperature display is blinking  and other digits will go off      3 Press key again    4  The top  third  digit of        density display is blinking  other digits will go off    5  Set the displayed value at 4 5  of the actual measured value with        key    and      key  then press key      6 The display will automatically revert to the present temperature      Note                   calibration  if        density display is not more than 2 0   an error tone will be  emitted and the input data will be ignored  and the display will revert to the present  temperature              calibration should be done after        density measuring was done at least three times   This calibration is available when the       display is more than 2 096     his unit has auto zero adjustment  make sure        gas is not full in the plac
53. mains     Control  ON OFF type  when the When set at 0 0          display is disappeared  and then  control is also off     Control OFF   When set at 0 0          display is disappeared  and then control is also off     Alarm  When the internal        density deviates from the SV 2 196  the digital display is  blinking  After 15 min  later  buzzer will sound intermittently and remote alarm  terminal will turn on       Check mode for overheat protection temperature  Setting             35      51  C    oetting method   Press the SET key triple to display the overheat protection set  temperature on the temperature digital display and      on the        digital  display   Press SET key again to return PV display mode               5  Self diagnosis function  When the sensor has any faults and        tank is empty and so on  error code and      is  displayed alternately  Buzzer will sound and remote alarm terminal will turn  on    Error code   E01         tank is empty   E05  Internal temperature control sensor is open circuit   E06  Internal temperature control sensor is short circuit   E07  Sensor of        sensor box is open circuit   E08  Sensor of        sensor box is short circuit   E09  Ambient temperature sensor is open circuit   E10  Ambient temperature sensor is short circuit   E11         sensor output voltage is abnormal   E12  Main heater is abnormal   E13  Bottom heater is abnormal   E14  Door heater is abnormal   E15         sensor box heater is abnormal   E16  SSR
54. mentioned above     Note   Always insert the fan on the motor shaft surely  Improper insertion may cause poor performance         76           ROUTINE MAINTENANCE    Filling the humidifying pan      Replace the water      the humidifying pan when the water level lamp lights       fill the humidifying pan        the following             1  Lift the front side of the humidifying pan cover as shown in Fig  1   2  Pull out the humidifying pan toward you  SeeFig 2     3  Dispose of the water in the pan and wash it with neutral detergent  Then rinse the pan with distilled  water sufficiently  Finally  wipe the pan with a soft cloth and alcohol for disinfection       4  Wipe off the bottom of the chamber completely     5  Place the pan under the pan cover and pour the sterile distilled water  about 5 liter  into it  See Fig  3   Make sure that the water is pre heated at 37 C     6  Push the pan in to its correct position and replace the cover over the pan   T  Check that the water level lamp on the control panel is off   Note     The sterile water filled in the humidifying pan should be pre heated at 37  C  Cold water lowers the  chamber temperature       PUR       LLL                  Hum          CALIBRATION               Temperature calibration    1  Press the CAL key for approximately 5 seconds    2  The third digit of the temperature indicator flashes  and the       density indicator goes out   3  Set the present correct temperature with the PP key and    key  then press the E
55. mes detached     Disconnect the power supply plug before moving the unit  Take care not to damage the power cord   A damaged cord may cause electric shock or fire     o Empty the humidifying pan completely before moving the unit  Spilled or splashed water may  cause current leakage or electric shock     9 Be careful not to tip over the unit during movement to prevent damage or injury   e Disconnect the power plug when the unit is not used for long periods     S Do not put the packing plastic bag within reach of children as suffocation may result              CAUTIONS FOR USAGE    1  Install in an appropriate environment  With an automatic calibration function  the        sensor 15 calibrated by utilizing air from the local  environment  Place the unit in an environment which will reflect normal atmospheric conditions        2  5  C higher than the ambient temperature   The chamber temperature must be at least 5 C higher than the ambient temperature  For example  the  chamber temperature is set to 37  C  the ambient temperature must be less than 32  C  Ensure the  ambient temperature is within the desired range     3  Do not subject to direct air flow  Do not place the unit in the direct air flow from an air conditioning system          air from an air  conditioning system may cause condensation and lead to possible contamination     4  Always keep the chamber clean  The Petri dishes or bottles for culturing may cause contamination in the chamber  Clean the containers  before 
56. mperature  5     to 35        PERFORMANCE       Temperature   5  C to 35  C  Humidity  equal or less than 80               Usable environment condition                   Temperature distribution 5 0 250        ambient temperature  25 C  setting  37 C  5   no load  Temperature variation ambient temperature  25      setting  37      596  no load      CO   control range 0 to 2096    CO   variation 2      0     ambient temperature  25      setting  37      5        load      30 dB     scale     Note  Theunit with CE mark complies with EC directives 89 336 EEC  93 68 EEC and 73 23 EEC     It is based on our measuring method               CAUTION    Please fill in this form before servicing   Hand over this form to the service engineer to keep for his and your safety        Safety check sheet    1  Refrigerator contents               LINo    Risk of infection     Yes          Risk of toxicity   Yes L  NO  Risk from radioactive sources     Yes            List all potentially hazardous materials that have been stored      this unit    Notes      2  Contamination of the unit    Unit interior   Yes LINO  No contamination   Yes LINo  Decontaminated  Yes                                     Yes LINo  Others     3  Instructions for safe repair maintenance of the unit  a  The unit is safe to work on    Yes   INo  b  There is some danger  see below    L Yes UNo  Procedure to be adhered to in order to reduce safety risk indicated in b  below     Date     Signature  Address  Division    Teleph
57. n  it may be best to install the unit in a sterile room     4  Use clean containers  Contamination is often caused by containers such as Petri dishes or bottles stored in the chamber  introducing contamination  Always keep the containers clean       Installation    1  Remove the packaging materials and tapes  Remove all transportation packaging materials and tapes  Open the doors and ventilate the unit  If the  outside panels are dirty  clean them with a neutral detergent and rinse with clean water     2  Adjust the leveling feet  Extend the leveling feet by rotating them counterclockwise so they contact the floor or bench  Ensure       unit is level     3  Fixthe unit  Two fixtures are attached to the rear of the frame  Fix the frame to the wall with these hooks and rope or  chain     4  Ground  earth    NWARNING    Use a power supply outlet with ground  earth  to prevent electric shock  If the power supply outlet is    not grounded  it is necessary to install a ground by qualified engineers   Never ground the unit through a gas pipe  water main  telephone line or lightning rod  Such  grounding may cause electric shock in the case of an incomplete circuit                                                                Sterilizing of chamber and attachments    Before first start up of the unit  the chamber and internal attachments should be cleaned and sterilized as       follows     Note   Take care not to damage the UV lamp or water level sensor at the time of removal or rep
58. ncreases     n Press ENT key  Set       density is memorized  050           Adjust upper alarm temperature set In        density indicator       is displayed        7         so that      alarm temp  is 1  C In temperature indicator  38 0 is    displa ied   280    higher than chamber temp   This is the end of set mode and the indicators   a              display current temperature and        density    In each set mode  the indicator returns to the current temperature and CO  density display mode                   Note     automatically when 90 seconds has passed without any key operation    In each set mode  if the change of the setting is not necessary  pressing SET key skips to next set mode   When the        density is set to 00 0  the control is OFF regardless of chamber density    The upper limit alarm temperature set value will change when the set knob is turned even if the unit is not  in set mode  because the alarm circuit is an independent circuit     OPERATING INSTRUCTIONS    A UV lamp is located inside the rear duct to sterilize the water in the humidifying pan and air circulating in       the chamber   Following shows precautions and instructions about the UV lamp       The UV light is exposed only to the inside of the duct and the humidifying pan cover when all chamber  components are installed properly      During cultivation  ensure all components are located adequately and never turn on the UV light without  the humidifying pan cover      Even if the unit is 
59. not page in service manual    but page in the lower corner of each page in the extract from user s manual   This page number is not corresponded with serial page number in Service manual         5                              20          INSTRUCTION MANUAL       CO  Incubator               EFI                     52                    Contents    Page   Ead     eee 1   Structural specification        2   Performance specification       n 3   Electrical specification   4                                                         7   Temperature calibration        9         calibration      10  Control specification      M H   mw 11  Wiring diagram   nn 26  Circuit diagram      27  Silk Print on PCB  E                                                           28  Connection on PCB  rc xc     29  Tubing diagram  mms 31                     _______ _ __ ___ _  ane 32                                    33  Specification of sensor   mmm 35  Parts layout m 36  How to replace UV lamp  mm 40  How to replace water level sensor  mm 42  TETTE ELEM  mE 43  Mounting procedure of        200        45  Honto stack      49    User s manual  m 51    Note     1  No part of this manual may be reproduced in any form without the expressed written  _ permission of SANYO     2  The contents of this manual are subject to change without notice     3  Please contact SANYO if any point in this manual is unclear or if there are any  inaccuracies     SANYO Electric Co   Ltd  All rights reserved  Printed in Ja
60. nsit indicator blink  15 minutes delay   When there is no key pressing in EE  ro ME mode   is  each setting mode for 90 seconds  Normal display mode  Ce ANE    When the key lock is                lt  ______  The setting is disabled     Normally  the zero point of the               sensor is calibrated every 4 hours         The decimal point  period   dde calibration very 10 minutes for the first hour after   on the CO   density  switch ON   using the atmosphere as indicator blinks   the gas to be calibrated         E01 is displayed alternatel       gas Cylinder If the CO   density does not Increase with the         ihe Intermittent tone  empty when the gas valve is opened  temperature           E01 is displayed alternately  with the temperature on the  temperature indicator    Gas supply line indicator  blinks   205 is displayed alternately  with the temperature on the  temperature indicator    E06 is displayed alternately  with the temperature on the  temperature indicator    207 is displayed alternately  with the temperature on the  temperature indicator    E08 is displayed alternately  with the temperature on the  temperature indicator      9 is displayed aiternately  with the temperature on the  temperature indicator    E10 is displayed alternately  If the ambient temperature sensor is with the temperature on the    short circuited temperature indicator       E11 is displayed alternately            22  voltage of the CO   sensor with the temperature on the                
61. one        Product name   Model   Serial number   Date of Installation    CO   incubator MCO 20AIC        Please decontaminate the unit yourself before calling the service engineer       85             7    6  101283002    Recycled paper                86        SANYO Electric Co   Ltd   Refrigeration Products Division  1 1 1  Sakata Oizumi Machi     Ora Gun  Gunma 370 0596 Japa    Printed in Japan            
62. onjunction with the buzzer      The upper limit temperature alarm cannot be silenced with the BUZZER key      E01 is cleared automatically when the gas is connected correctly and the buzzer is silenced with the  BUZZER key   f one of E05 to E17  except for E12  E13  and E14  is displayed  consult with a Sanyo  sales representative or agent                            Gas supply line is    When the gas supply line is switched   altered      only when MCO 20GC is installed                Gas line switching Intermittent tone _             If the temperature sensor is  Chamber disconnected   temperature sensor    abnormality    Intermittent tone Heater OFF       if the temperature sensor is short  circuited    If the sensor box temperature sensor is  Sensor box disconnected  temperature sensor    abnormality          Intermittent tone        valve close        If the sensor box temperature sensor is  short circuited    If the ambient temperature sensor is  Ambient disconnected  temperature sensor    abnormality    Intermittent tone                                  ALARMS  amp  SAFETY FUNCTIONS       Remote alarm terminal          The remote alarm terminal and remote recorder terminals are located at the rear right side  _    Remote alarm terminal         The remote alarm terminal is a contact output   Normal   OPEN  Abnormal  gt  CLOSE  Contact capacity   DC 30V  2A  no voltage contact  When the power switch is OFF or the power failure condition  the contact output is CLOSE       
63. operating without turning on the UV lamp  always install the humidifying pan cover     the pan cover is not used this will affect the temperature distribution and humidify recovery      Even if the unit is operated without humidifying  always install the humidifying pan and the humidifying pan                               lamp is turned off when the outer door is opened during ON period      When checking the UV lamp operation  open the outer door and push the door switch with the inner door  closed      The UV lamp is ON for a predetermined period after the outer door is closed  The period is factory set at  5 minutes  Change the setting as necessary as shown in the table below              recommended timing of lamp replacement  the ratio of UV output is less than 70  of initial value  is  when the accumulated ON time is over about 1 000 hours         blink of the UV indicator when the UV  lamp is OFF means the accumulated time has exceeded about 1 000 hours and recommends the  replacement of the lamp      Consult with a Sanyo representative or agent at the time of replacement of UV lamp     asic operation sequence  Example  change of UV lamp ON period from 5 minutes to 3 minutes       Description of operation Indication after operation                                 CAL The left digit in the temperature        3 A  d indicator is flashed  310    2  By pressing      key and    key  set        _   When pressed  the changeable digit is shifted   the figure to F01 LA  When p
64. out   5  Set the present correct CO  density with the        key and 4 key  then press the ENT key   6  The unit automatically reverts to the display mode       Note   This calibration is available when the setting of        density is 2  or more      Example    For an internal        density of 5 0   setting  and a measured value of 4 595    1  Press the CAL key for about 5 seconds    2  The third digit on the temperature indicator flashes  and the CO  density indicator goes out    3  Press the CAL key once again    4  The third digit on the CO  density indicator flashes  and the temperature indicator goes out    5  Set the present correct CO  density  4 5   with the PP key and         then press the ENT key   6  The unit automatically reverts to the display mode       78        TROUBLE SHOOTING       If the unit malfunctions  check out the following before calling for service     The unit does not operate at all   1  Is the unit plugged correctly into a power outlet   2  15 the circuit breaker at the power source active   3  Has a power failure occurred     The key operation is disabled  1  Is the key lock function set in OFF mode     If the alarm function operates  If the alarm function and the buzzer operates  check the cause using the following procedure    At the beginning of operation     1  Is the chamber temperature equal to the set value   2  15 the chamber        density equal to the set value   a  Is the secondary pressure of the pressure regulator equal to the se
65. p flashes when the water in the humidifying pan is less  than approximately 1 liter      6  Upper limit regulator  This regulator is used to set the upper temperature limit    7  Over heat lamp  OVER HEAT   This lamp lights when the chamber temperature reaches the upper  limit set value    8  CO  inject lamp  INJECT   This lamp lights when        gas is being injected    9  Digital CO  density indicator            Normally  this indicator shows the       concentration in the  chamber       the setting mode  it indicates the set value of the        concentration    10  Set key  SET   Pressing this key to enter the setting mode  and the digits to be set will flash    11  Calibration key  CAL   By pressing this key for approximately 5 seconds  the unit enters calibration  function mode  Used to change the UV lamp ON period    12  Alarm buzzer stop key  BUZZER   Press this key to silence the buzzer when the alarm operates and  the buzzer sounds  The remote alarm also stops by pressing this key  Press it once again to reactivate  the buzzer    13  CO  gas supply line indicator          The lamp for the supply line currently in use lights up provided  that MCO 20GC changeover accessory is installed  This will flash when either gas cylinder is empty    14  CO  gas supply line switching key  This key to select        gas supply line is available only when an  automatic cylinder changeover MCO 20GC  option  is installed  When one       cylinder is empty  the  CO   is supplied by the o
66. pan        CONTENTS   PRECAUTIONS FOR SAFE OPERATION    2   CAUTIONS FOR USAGE P  6   INCUBATOR COMPONENTS P 8   INSTALLATION   P  11  BEFORE COMMENCING OPERATION P  13  OPERATING INSTRUCTIONS P  16  ALARM  amp  SAFETY FUNCTIONS P  20  ROUTINE MAINTENANCE P  22  CALIBRATION P  26  TROUBLE SHOOTING P  27  ENVIRONMENTAL CONDITIONS    P 28  DISPOSAL OF UNIT P  28  STACKED MODULE P  29  SPECIFICATIONS     31  PERFORMANCE P  32    SAFETY CHECK SHEET Piao    PRECAUTIONS FOR SAFE OPERATION       It is imperative that the user complies with this manual as it contains  important safety advice     Items and procedures are described so that you can use this unit correctly and safely   If the precautions advised are followed  this will prevent possible injury to the user and    any other person     Precautions are illustrated in the following way       NWARNING    Failure to observe WARNING signs could result in a hazard to personnel  possibly resulting in serious injury or death      NCAUTION    Failure to observe CAUTION signs could result in injury to personnel and  damage to the unit and associated property        Symbol shows   X this symbol means caution   S this symbol means an action is prohibited     e this symbol means an instruction must be followed     Be sure to keep this manual in a place accessible to users of this unit     PRECAUTIONS FOR SAFE OPERATION   NWARNING    Do not use the unit outdoors  Current leakage or electric shock may result if the unit is exposed to      
67. provenant de l int  rieur de l appareil quand  vous ouverz la porte        Use this unit in a safe area if using poisonous  harmful or radioactive substances                   use may be harmful to your health or the environment        S Never expose the eyes directly to UV light as UV light can cause permanent damage to eyes     e Disconnect the power supply to the unit prior to any repair or maintenance in order to prevent  electric shock or injury        9 Ensure you do not inhale or consume medication or aerosols from around the unit at the time of  maintenance  These may be harmful to your health          Never splash water directly onto the unit as this may cause electric shock or short circuit         Never disassemble  repair       modify the unit yourself         such work carried out by an  unauthorized person may result in fire or injury due to a malfunction     e Disconnect the power supply plug if there is anything wrong with the unit  Continued abnormal  operation may cause electric shock or fire     o If the unit is to be stored unused in an unsupervised area for an extended period  ensure that children  do not have access and that doors cannot be closed completely     The disposal of the unit should be undertaken by appropriate personnel  Remove doors to  prevent accidents such as suffocation        Prepare a safety check sheet when you request any repair or maintenance for the safety of service  personnel          56        PRECAUTIONS FOR SAFE OPERATION     
68. r  and the  electricity conductivity ratio for    F07    is applied in the same way as bottom  heater   However  electricity will be conducted with different rate from main heater  for the following cases   1 Until temperature reaches within  0 2     from the set temperature after  turning on power          order to prevent inside temperature from rising  electricity is  conducted with approximately 40  of main heater conductivity ratio   2 When the set temperature is more than 40         n order to prevent inside temperature from rising  electricity is    conducted with approximately 80  of door heater conductivity ratio     13  Remote Alarm  Remote Alarm Contact    Normal      RCM RR ORDIN OR                           NUR                     M NC Open  Upon alarm and blackout          Close    Remote alarm is connected to buzzer sound     14  Examples for displays          Densin  5  0            36  2         37        FOS Flas Auto Zero Adjustment    Decimal point is blinking   Service Code Input         CO  density Zero Adjustment  5021    004 CO  Gas Error  EO 1    Lock Mode LO gH        24        15  Humidity pan water level sensor    Water level sensor is for checking amount of water in humidity pan   It checks at start up  open close door and every 30 minutes   The amount of water   Large           Alarm lamp of humidity pan water   OFF    The amount of water   Less than 1L           Alarm lamp of humidity pan water   Brink         Wiring diagram                     
69. rature of        sensor box is stable  and when internal temperature is not in high low temperature warning and also  when        sensor output voltage is lower than 1000mV or higher than 4800mV  It  displays  E11  alternately with  internal temperature  and sounds buzzer  at  intervals   At the same time CO  valve is CLOSE     For your information         sensor output voltage is 863V in the situation of         density is 0   The value is applicable only in that temperature of        sensor box is  stable at 45          E12 Main heater is abnormal  When upper limit temperature alarm is activated or when main heater is open  circuit or when main heater SSH is short circuit  it displays  E12  alternately  with  internal temperature  and buzzer sounds  at intervals    Self diagnosis for SSR and heater failure is done every 40 minutes  therefore error  is not displayed just after failed   For E13     E16  refer to this explanation     E13 Bottom heater is abnormal  When bottom heater is open circuit or SSH is short circuit  it displays  E13   alternately with  internal temperature  and buzzer sounds  at intervals      E14 Door heater is abnormal  When door heater is open circuit or SSR is short circuit  it displays  E14   alternately with  internal temperature  and buzzer sounds  at intervals        15        sensor box heater is abnormal  When        sensor box heater is open circuit or SSH is short circuit  it  displays E15  alternately with  internal temperature  and sounds 
70. ressed  the figure of settable digit increases     The current setting is displayed mo ad  3  Press ENT key  ENT  in the       density indicator  005    When pressed  the changeable digit is shifted                When pressed  the figure of settable digit increases   Set value is memorized and the display return to  normal display mode   Note     The available set range for the UV lamp is between 0 and 30  000 to 030   When set to 000  the UV lamp  is not turned on       By pressing      key and 4 key  set  the figure to 003        LAA CI ES    Condensation will occur and or temperature distribution may be affected due to the heat of the UV lamp  when the setting of the lamp operation is longer than 5 minutes or if the outer door is opened repeatedly         69                OPERATING INSTRUCTIONS       Key lock function    This unit is provided with a key lock function  When the key lock is ON  change of temperature or CO   density setting through the key pad is not available   Note  The key lock is set in OFF mode at the factory           Key lock is OFF Enable to change of temperature setting  Key lock is ON Disable to change of temperature setting                      Description of operation Indication after operation      The current chamber temperature is displayed    Diese bb hey tons seconds  LO is displayed in the temperature                    indicator     3 Press key and scroll the figure   When pressed  the figure of settable       to 1  digit increases    The
71. s       4  Remove        and fix   with                5 Connect the wiring harness of CO   valve B to wiring harness from      9 on Main board    6 Disconnect upper tube of CO   valve A    Connect        valve A and        valve B and tube X by using 2 as shown in Fig 3   7                bottom tube of    to      8 Connect 3 with CO   gas supply line B   9 Fix tubes securely using    and   as shown in Fig 3   10 Replace the rear cover     NOTE  Every tubes should be connected surely to prevent        leakage                                        gt  2           1   i             Main  board                                supply line     m  yellow  CO   gas  supply line B    Setting procedure of Control panel   When use MCO 20GC the automatic switcher of        gas supply line  it is necessary to set the  MCO 20AIC automatic gas switching mode according to the following procedures     operated       the power switch       The current chamber temperature  displayed     Press CAL key for 5 seconds  The left digit in the temperature indicator  CAL is flashed   By pressing      key and 4 key    gt  set      figure to                 4   Press ENT key  In        density NIE    right digit is flashed   5   By pressing p    key and 4 key   set the figure to 384    Press ENT key  The current chamber temperature is  displayed     7   Press CAL key for 5 seconds  The left digit in the temperature indicator  15 flashed     By pressing      key and    key   set the figure to F08       
72. s cylinder B  lamp should be on     stably     NOTE         density control is interrupted when automatic calibration of CO    sensor is activated and the decimal point of CO   density indicator blinks   All the procedure are completed   Shut down the CO   supply of cylinder A and cylinder B   Turn off  E01  display with the buzzer key and select cylinder    with CO   gas  supply line switching key   Turn off the main power switch   For proper use  refer to the instruction manual of MCO 20AIC        i Check the        gas density indicator reaches to the set value 5  and keep it         How to stack        20       iil    This unit can be stacked by using the stacking kit  Following shows the procedure for stacking the unit   Consult with a Sanyo representative or agent prior to stacking procedure as such work involves dangers      NWARNING      Select a level and sturdy floor having enough strength for installation of stacked module        Never stack 3 or more units   Take care not to drop or tip over the unit when stacking as this can cause injury or damage of the unit     1  Turn off the power switch and disconnect a plug of each unit     2  The stacking plate A and B are attached on the back of each unit  To prepare the stacking plate A and  B  remove the rear cover by unscrewing 6 screws on the rear cover  See the figure below     3  Take out the stacking plate A and B by loosening 2 fixing screws on each unit   4  Replace the rear cover and fix it with 6 screws     5  
73. sensor  Electrical independent circuit   Over heat protection Manual setting type  lamp turns on  buzzer sounds continuously   heater is OFF   When outer door is opened  Inside fan OFF    CO   valve OFF  DOOFSWITER   Heater OFF  when opening period is  longer than 1minute   When alarm is activated  remote alarm ON   Remote alarm  Alarm and buzzer are interlocked    Contact output  rating of contact DC30V 2A    Error code and internal chamber temperature are displayed  oelf diagnosis function   alternately   Buzzer and remote alarm contact torn on             CO    Gas inlet    Glow starter    Mains cable    Sample port                      480              LLL                                 Remote alarm termind    880    MMos                     OS           EERIE    NY                           ee ee              at    a bs                              Monitoring hole                                      7   E ELS           Overheat protect sensor          B ie Y        as   N Temp  control sensor N                      Z S P D 3 M 5     ts N 5  7 5    is    Rear duct Z     R Sensor S   di          2        CO2 INJECT S       Humidifying 7                          E                2 ANB      Sample        553            SFL          216   T      7 77 N    O E E                      water level sensor    Humidifing pan      _ Electrica Parts       MCO 20AIC AC220V 240V 50Hz    J    IO       lt              o  5      o             12     Temp  control sensor            Type 1
74. sh products  ID for serial communication  and pushing ENT  key  you can set serial communication ID     The range is  000   255   000  turns communication off     F22  Purpose  Set the mode for serial communication    How to use  Enter    F22    in the temperature display and push ENT key          then CO  display shows  000   Then  changing the third figure  you  can change communication modes     0    is a local mode  and  1  is a  remote mode   Also  changing the second figure  you can change the baud rate     0       is 2400bps     1    is 4800bps            2    is 9600bps     Control Mode  third figure  O Local  Initial value   1  Remote   baud rate  second figure  O 2400bps  1 4800bps  2 9600bps      22        11  Bottom heater control    In order to shorten the recovery time of humidity  conduct electricity to  humidity heater as follows     When inside temperature is within  0 4    0 6 degree from the set  temperature  conduct electricity to humidity heater not more than 10  minutes  Except for this case  the set value for    F06    is applied     However  you should not conduct electricity continuously for the following  cases    1   Inside temperature is not yet within  0 2 degree from the set  temperature after turning on power    2   Inside temperature is not yet within  0 2 degree from the set   temperature after continuously opening a door for more than 60    seconds     12  Door Heater Control    Basically  electricity is conducted the same ratio as main heate
75. stallation  This precaution will prevent the unit from tipping   Improper installation may result in water spillage or injury from the unit tipping over        5  Alocation without flammable or corrosive gas     N WARNING    Never install the unit in a flammable or volatile location  This may cause explosion or fire     Never install the unit where acid or corrosive gases are present as current leakage or electric shock  may result due to corrosion        6  Alocation not prone to high humidity   N WARNING    Do not use the unit outdoors  Current leakage or electric shock may result if the unit is exposed to rain        water   Never install the unit in a humid place or a place where it is likely to be splashed by water   Deterioration of the insulation may result which could cause current leakage or electric shock           63           INSTALLATION    Prevent contamination    To prevent contamination of the chamber  select an appropriate location for installation as well as ensuring       the complete disinfection of the chamber components     1  Avoid hot and humid location  Avoid location with high temperature and or humidity as the presence of bacteria in the air is greater than in  the normal environment       2  Avoid drafty location and location with many passers by  Avoid locations near doors  air conditioners  fans  etc   where slight breezes can facilitate the entry of  bacteria into the chamber     3  Installation in a sterile room  To promote efficient cultivatio
76. storing them in the chamber     5  Allow adequate space between the cultures   When storing cultures in the chamber  keep the Petri dishes or bottles containing the cultures sufficiently  apart from each other to allow adequate air circulation  Inadequate space may result in uneven  temperature distribution and       concentration in the chamber     6  Always shut the inner door   Shut the inner door completely  and then shut the outer door      the inner door is not closed completely   even if the outer door is closed  the unit will fail to exhibit its maximum performance  Ensure you close the  doors gently  Robust closing may cause spillage of medium  incomplete closing  or damage of gasket   Before opening the inner door  check that the UV light is OFF     7  Keep the inside panels dry   To protect the inside of the unit from contamination  the inside panels should always be kept dry  If water  is spilled from the humidifying pan or if the door is kept open for a long period  condensation will form on  the panels  allowing bacteria or fungi to breed  If this occurs  wipe away the water with a dry sterile gauze   Particularly  if the medium is spilled  wipe it up immediately and sterilize the area     8  Fill the humidifying pan with sterile distilled water   Always use sterile distilled water to fill the pan  The RH PAN light on the control panel flashes when the  water level is low          the sterile distilled water to the pan when the RH PAN light blinks  Note that  wh
77. t value  0 03MpaG  0 3kgf cm G   4 3psiG          b  Is the tube connected securely between the pressure regulator and the unit    During operation   1  Is the upper limit alarm temperature set at least 1  C higher than the set chamber temperature   2  Was the set temperature value changed  or the door left open for a long period  Was a low  temperature load placed inside the unit  In this case  if the unit is left as it is  the alarm will eventually  clear itself     3  Has the gas tube slipped off   4  Was the set value of the gas density changed   5  Is the gas cylinder empty  Check the primary pressure of the        cylinder once a week     If the chamber temperature is not equal to the set temperature     1  Is the temperature in the vicinity too high         ambient temperature must always be at least 5  C less  than the set temperature    2  Was the outer door closed while the inner door was left open     If the gas density does not coincide with the set value  1  Is the secondary pressure set to 0 03MPaG  0 3kgf cm G  4 3psiG    2   5 the gas tube clogged or chinked     If the chamber humidity does not rise  1  Is the humidifying pan filled with sterile distilled water   Always use sterile distilled water    2  15 the humidifying pan cover installed correctly     If the CO  consumption is too much   1  Is the door opened frequently    2  Is there any gas leakage at the connection or pin hole on the tube  It is recommended to replace the  tube once a year    3  15 the 
78. the density rapidly     Ex   0 096 BE Ton 1 096        density     The added value is 0 25    ours     5  Auto zero adjustment OFF setting  Auto zero adjustment could be set OFF by special function F08 In case that auto zero  adjustment does not be set  you should observe CO   density that changing as time goes  by         33               gas density calibration and auto zero adjustment    MCO 20AIC has two functions  one is        gas density calibration by hand  and the other is  auto zero adjustment     1         gas density calibration  When either the internal        density set value or the display is not match with the actual    value  do        gas density calibration according with        calibration   Page 10  to input  the actual value again     2  Auto zero adjustment  Hefer to  Auto zero adjustment   Page 33     3  Relationship to two these functions   It could be described as follows   for example  5  CO      Ideal line for the set value and the actual   value   n ERE E             Note       fact  it could not make       Straight line due to the characteristics of    ox the sensor         Point calibrated by CO2 gas density   gt  calibration                                    lt   0       5    Set density  96CO       Point calibrated by auto zero adjustment         Specification of sensor       B The following shows temperature and resistance characteristics on thermistor sensor  103AT 1                                              7 19  6 94  6 70  6 47  6 25
79. ther cylinder automatically  But if necessary  by pressing this key  the supply line  can be switched between A and B manually    15  Enter key  ENT   Pressing this key memorizes the set value in the controller    16  Numerical value shift key  4    Pressing this key in the setting mode causes the numerical value to  shift       key lock mode  pressing this key makes key lock ON or OFF    17  Digit shift key  PP   Pressing this key in the setting mode causes the changeable digit to shift   Pressing this key more than 5 seconds enters key lock mode         page 18 for the key lock             INSTALLATION    To operate this unit properly and to obtain maximum performance  install the unit in a location with the       following conditions   Note  The ambient temperature must be at least 5  C lower than the set temperature     1     location not subjected to direct sunlight or direct air flow from an air conditioner    2  Alocation with clean air and adequate ventilation  Small and sealed room is not recommended       N WARNING    If the unit is used in a small confined room  the CO  density level in the air could rise and may be  harmful to humans        3  A location away from heat generating sources    4  Alocation with a sturdy and level floor     N WARNING    Install the unit on a sturdy floor   f the floor is not strong enough or the installation site is not adequate   this may result in injury from the unit falling or tipping over    Select a level and sturdy floor for in
80. tions for mold increasing  There are many cases that mold is increasing by eating fat on the fingers as a source of  nutrient in        incubator   Therefore           sure to wash and sterilize your hands prior to servicing  Use 7096 ethanol   2n installing  sterilize the inside wall and shelves after removing shelves  duct   humidifying pan and so on with 7096 ethanol      3 In the case that mold is already increasing   oterilize the interior  walls  and shelves with 7096 ethanol after removed all items from  the chamber as same as  2  2  above     And you should sterilize again after 24 hours once sterilized  when the root of spore is  geminated  because it is too difficult to remove spore of mold  Repeat sterilization is  effective     NOTICE  In general  stainless steel is known that itis not easy  hard  to rust   However depends on the conditions  rust might generate   When you remove rust  we recommend the following agents   Besides  the surface may become foggy   Hecommended agent   1  Cream cleanser  Gif   Unireva     2  Picasso  SUS300 W  Chemical YAMAMOTO     Picasso has great capability of clean for rust than Gif   gt     or           Auto zero adjustment      MCO 20AIC uses the infrared        sensor and it has to be adjusted        density and output  voltage  that they are changing as time goes by       the first stage  when mains on   auto  zero adjustment is carried out every 10 minutes and repeats this cycle six times  total 1  hours   then auto zero adjustm
81. tor    Lead wire hole  Insulation material    3 Remove the duct from inside the unit and remove  UV lamp cover    4 Hold the socket part of UV lamp and pull toward  you  Take out both UV lamp and the socket     5 Set new UV lamp  with socket  to the hole and make  sure that it is fitted properly    6 Place UV lamp cover and set back the duct      Insert the insulation material to the hole of UV lamp  lead wire at the backside of the unit and connect  with the lead wire    8 Set the rear cover    9                the glow socket  locate as Fig  1    10 Clear the accumulation time of UV lamp  Set  function to   04 000     glow starter    glow socket       UV lamp cover    Fig 2    10                  accumulation time of UV lamp     Description of operation Key Indication after operation  operated    1   Turn the power switch ON  The current chamber temperature is  displayed     2   Press CAL key for 5 seconds  The left digit in the temperature indicator  CAL is flashed     3   By pressing      key and 4 key      set the figure to               4   Press ENT key  In        density indicator      000  is displayed and the          right digit is flashed     5   By pressing p    key and  amp  key      Z2 M  Press ENT key  The current chamber temperature is  ENT displayed  7   Press CAL key for 5 seconds   CAL  By pressing        key and    key      set the figure to 204       i         The left digit in the temperature indicator  is flashed    Press ENT key  In        density ind
82. use excessive force on the sensor during maintenance     17  Remote alarm terminal  Refer to page 21   18  Access port  When not in use  cap with the rubber cap on both outside and inside     19  Power switch  Main switch of the unit  Also  functions as an over current breaker  15A   The  switch is covered by a switch cover to prevent the  accidental push       turn on or off the switch  remove  the switch cover by loosening the screw  See figure  on the right     Switch cover    20  Glow lamp  For UV lamp  model  FG 7P          Gi                                            5    Control panel and keypad       3 2 1 8 9  O UV    HEAT    INJECT  4    DOOR                 5                  ILI J  LI  6        OVER HEAT TEMPERATURE            11  GAS SUPPLY 12  OATBO  7                           4  ez                          55   F   on  A     10 17 16 15 14    1  Digital temperature indicator  TEMPERATURE   C   Normally  this indicator shows the chamber  temperature  In the setting mode  it shows the set value of the chamber temperature      the self  diagnostic function detects any abnormality  an error code will be displayed    2  Heater lamp  HEAT   This lamp lights when the heater is energized    3  UV indicator  UV   This lamp lights when the UV lamp is ON         blink of this indicator recommends  the replacement of UV lamp         page 17 for the details    4  Door lamp  DOOR   This lamp lights when the outer door is open    5  Water level alarm lamp  RH PAN   This lam
    
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