バラスト水管理システムの承認の際の 生物分析方法（改訂版）
Contents
1. 42000000 4 21000000000000000000000000000000000000 4 22 00000000000000000000000000000000000 aj 0000000000 d2. 562000000 5 6 2 1 00 5 6 2 11 0000000000000000 000000000000000000
3. 558 5 5 9 0000000000 5 5 109 00000000000000000000000000000000000 5 5 11 5512 5 56100
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5. 72 0000000 73 020 mH 0000000000 0 0 0 000 Op 74 100 m 5 0102 02 1 1
6. 00000000N 52 00000000L 000000000 0 D Uu 0 4 14 2 0 5 1 5 11 00000000000000000000000000000000000 0000005 20 000000000 5 3 6000000000000 00000 5 4 0005 50 0000000000005 6 060000005 0000000000005 8 5 1 20 0000000000000000000000000 0000000000
7. Q17 1000 100 30000000000 U U D Minimum Dimension J lou m0 D it 00000000000000000000000000 000000000000 98 018 D0000 00000000000000000000000000 019 00 00 0 DD
8. ES 15 E I2EZT EZJ E Ez L3 r3 rr E Ez Ez ES E LIE J E L3 r3 rr ELE DU Uu OG aag ooo ono tr aag ogo 9 oa oa oa oa 64 5 10 20 0 00000000000000000000 103100080000 0 0000 0 0000 DU Uu 5 10 3 00000 D uuu aj 510 5 0
9. 2 D uuu D uuu DU uuu D uuu DU uuu 0 1 30 0000000 2 100 6 D 2 1 3 0000 2 0000000000000 20 0000 20090 0000000 6 pD 20 00000 2100 D 2 20 0000 p21 00000 somp O00000000L0
10. uU H 000 000 00000 Noctiluca scintillans 150 2000 Tintinnapsis spp 15 167 Tintinnidium mucicola 50 160 Favella taraikaensis 70 90 Favella ehrenbergii 80 95 Vorticella sp 38 200 Didinium balbianii 60 96 Didinium gargantua 125 130 Keratella cruciformis 40 100 Synchaeta 5 95 145 Brachionus plicatilis 110 176 Oithona dav sae 140 140 uui St cholonche zanclea 200 300 utl sp 300 5000 Oikopleura dioica 500 130 DU pol yphemoa des 500 700 Arcella discoides 90 146 Arcella sp 90 216 Arcella vulgaris 100 152 30 4 1 2 5 000 000 000 Chaetoceros compressum 3 40 Eutintinnus spp 18 53 Rh zosolen a setigera 3 87 Helicostornella longa 17 18 Aulacose ra distans 4 20 Stenosemella parvicollis 19 25 Chaetoceros sociale 4 15 Stenosemella sp 19 47 Aulacoseira granulata 5 20 Helicostomella subulata 20 24 Chaetoceros radicans 6 25 Tintinnapsis aperta 25 25 L epto
11. 0 0 4 42 00000000000000000000000000000000000 4 4 5
12. 5 10 3 25 10 20 0 5 1031 510400000 E EES 5 10 4 15 1 2 D Uu al 0 0 0 0000000000000000005 6 2 1 1 000000
13. 0 54 5 3 55 5 000 DU uuu 0 0 DU Uu U Uu
14. 0000 00 00000 0 C 5 Q 2 FOO OM L3 L3 o Oy F J 1 aa 11 O LE EAE E L3 rrr E JN oA E L1 L3 L3 r3 E3 E pz Ep d E 4 8 000 D D 4 10000 LJ Ld ET EJ 4 9 4 9 1 a 0 000
15. 52 DU d D uuu e 0000000000000000000000000000000000000 f mM iD AR CA
16. Im 1x35 05096 i 150m 150m S 0509162 0000 Im 15 10 OOOO 050916 L 1600 1505 150 OUUU U U S 0509169 0000 Im 15 20 __ 2 Total T 83 13 2005 9 17 2005 9 18 01 13 0 03 84 2 00000000 215 1000 ml 22 30 Dinophysis rotundata Ditylum brightwellii Skeletonema costatum 1 5 1 ji os
17. 12 000000000000 D 200000 00 00000000 52 WHO Laboratory Biosafety Manual O0 HL BB BH BH BH BH B D UU U 2 0 000000000000 0 00000000000000000000000 0 00 1509001 0 O 7025 0000000000000 0 088208 00 000000000
18. HOU 39 00 0 35 50 m 000 m 0 D uuu 41 0000000000 97 4 5 413 10 UUU U D Eucampia 00000 0
19. 8 5 O minimum dimension ED E E H E E U U U Lr 414 O EJ Qi 015 0 00 mt D DD D U U A 00000 016
20. 14 2 000000000 8 000000000 0000000000 00000000 000 5 6 100000000000 21 60000000000 U 00000 Im 403UUUUU 5 00000 60000300000 000 560000 6000 0 0 2 5 0 0 0003 D uuu D Uu Dwg 1
21. 4 19 3 000000000600 0 00 4 104 D uuu 4 10 5 000 411 4 11 1 D Uu D uuu 411 2 UU
22. 2 U UU 4 1 2 Sonum D 4111 3 100000 22 4 1 2 D D minimum dimension 000 1 4 1 1 2 4 13 M 41 1 01006 000 00 s x ud p 4i2 UDUUDUUDUJa 0006 000 DDD z 00000 g d e ame AE T ege z r cd 01 4 1 3 000000000 0006060 HO D b 000 23 1 U minimum dimension O 0
23. 010 000000 000000000 00 viable organisms d 000000 O00 minimum dimension 1 L1 E 0 7 0 0 0 0 2 100 D22 1000000 U Escherichia coli O O Intestinal Enterococci 0 O Toxiogenic Vibrio cholerae O1 and O139 0000000 8 2 3 20 DH E HE E UU U Coliform U U U U UEnterococcus group brio cholerae O Heterotrophic bacteria O o pgadgdgnmnnu ug 000 O ULU D D cholera enterotoxin O0 000000000 Heterotrophic bacter
24. lu 7 DU 00 L 7 000000 0000 p pnunupnupnuugug W 7 180 3 19 190 3 International Convention for the Control and Management of Ships Ballast Water and Sediments Resolution MEPC 125 53 Guidlines for Approval of Ballast Water Management Sys
25. 510 42000 0 0 64000000 000 0 D Uu 0 0 DU Uu 0
26. 0 0 DU aj 05 4201 H DE 5 4 2 p H 47
27. 0 49 00000000000000000000 d dj L3 E L3 r3 r3 r3 EFER E 1 L3 EJ rr E L3 r3 r3 4 10 000 4 10 1 4 10 2 000
28. 40 U HD TSS DOCH POC 44 1 js U O 0 1 C U U U 1PSU 0 1 DOL 0 1mg t 0 1 4 4 2 000000 TSS
29. 2 00 2 1000000000000000 000000000000000000000 1 1 1 1 0 53 0 Be E H E EI 5 22 00000000000000000000000000000 000000
30. 0000 00000000 0000 91 006060 O00000 00000 M ni num uut 00 70 2 1 DDD 1000 pel vn AIIN e Q N o 24 well H D LU lI 00 500 mel 90 t 02 s 0 5 mt N N 7 00000 0000000000
31. 5 UUUUU DOCU 550 000000000000000000000000 5 D UU uL
32. 0 DU Uu 0 562 1 2 5 6 2 2 5 62 22 1 00 5 6 2 10 0000000000000000000000000000000 5 62 1 0
33. i 100000 20 0000000 2 000 D 2 10 000 L 2 3 DU DU 4l 90000000 0 000000 0000000 x000 si musn 500 504m0 10 ej 000000 D 55
34. 10 Q20 Minimum Dimension O O A 8 99
35. D Uu 4 12 00000 4 12 1 00 4 1211000000000 000000000000000000 00000000 4 12 12 4 12 13 4 121400 0000000 00000000000000000000000000 4 12 2 000010 4 12 2100 000000000000000000000000000000000 uU uuu uU uu 51 0
36. 10 4 1 40 0 00 4 1 5 0001 4 1 6 m 100 CO gm D 41 4 o e Dn bic e e e e e 01 4 1 5 000000000000 00000 01 4 1 6 600000000000 200000 100 0 b e 000 24 4 1 4 0 viability O10000 viability ID BH OL UO LU D viability 0 HH o0 ggi 41 7000 DDD DDDD 00000000 DDD P DDDDDDD DDDDDDDD DDDDDDDD 4 D a 011 4 1 7 20000 viability ag HEB viability viability E E EEEEEE FEE EHE TH I D uu
37. 1 Both a Quality Management Plan QMP and a Quality Assurance Project Plan QAPP Guidance on preparation of these plans along with other guidance documents and other general quality control information are available from appropriate international organizations ISO17025 2 2 The QMP addresses the quality control management structure and policies of the testing body including subcontractors and outside laboratories 3 The QAPP is a project specific technical document reflecting the specifics of the BWMS to be tested the test facility and other conditions affecting the actual design 12 0 and implementation of the required experiments 2
38. 1 E E D uu D uu E L1 L1 L1 L1 L1 L1 ass E L3 rrr 1E T L3 r3 rr Er L3 r3 rr E LE ELTE H L1 ESDSEST ET EI 111 7 EST Ez CEST ES ET L3 L3 E3 m L3 L3 E3 E3 E3 E3 EJ E3 E3 E3 EJ LI EJ 1 T EHE L3 L3 E3 E3 E ELE EEE E EINE EER ELEN E ECT ELE E3 E E ETE E3EAT EJEJTETE3E T E 1 1 san EZ ESSE E SEE CEZ ET ESSE EE ET SES ET L3 L3 E3 E3 E3 E3 E3 EJ E3 E3 E3 EJ E3 EJ EJ Ds EJ E33 E E E EET EN ET aa ETE E YET E ETE EST ET ETE TET EST E ESI ER esa Lr L J E r r3 0 0 0 0 0 L3 L3 L3 E3 EJ Lr Lr Lr pp Lr EKE EE Lr EI EI Lr
39. 50918 Iluun 5038 C 0000 ll Ml 8j S d utri 000000 s0918 unuuu unnowr3 j T 12 1 121 E E F T P 20L L D Lt LE E ECL LE E 5 LE E 5 Protoperisimium sp S0pm 1000 Coscinodiscus sp 50um 1000 cyphozoa polyp 80 10001m L1 10ml 78 un 5 iam OODI runi 0 L 13 00 pM Tintinnopsis sp 80um 1000 cyphozoa polyp 80 10001m 1 79 LI E E JL alala 5 lam LOODU 15 00 Coscinodiscus granii 50um Coscinodiscus sp 50um cyphozoa polyp 80 1000um olychaeta larva 60um elecypoda larva 60 200um Paracalanus sp 300um Oith
40. 000 20 00000220 0000098200 10 50 0 10 50 1 000 20 00000 20 00000920 0 0 00009 0009 o D 000900001 0000 DD 1 00020000 DOOD nm DHUBUSDHUDU 1 UHHH DUH UM LE a caa oe nct 50 D 5 0000000000000000000000 C 0000200000 0000900000 0000000000 9 67 e cuciograomamaamudmuapogougooos8BHounooooocaoaoaoaooouauogoooumouooaoug m aO i in in in im 00 eo em m C CP e AMANAN aaa ma FH N N N c
41. R2A 00000 Yeast Extract Acid hydrolysate of casein 0000 Glucose Soluble starch KHPO Sodium pyruvate Pancreatic digest of casein 000000 Peptic digest of animal tissue 0000000000 5 anhydrous 00 Agar Purified water 7 2 0 2 121 1 150 000000000000 Marine 2216 0000 00000 Yeast Extract 00000 Ferric citrate Agar Seawater or artificial seawater pH 7 640 2 121 1 150 0000000000000 30 31 0 5 0 5 0 5g 0 5g 0 3g 0 3g 0 25g 0 25g 0 024g 15 0g 1000m 5 0g 1 0g 0 1g 15 0g 1000 m 30 American Public Health Association 2005 Standard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 31 American Public Health Association 2001 Compendium of methods for the microbiological examination of foods 4th ed American Public Health Association Washington D C Colony forming unit 0 0000000000000 00000000000000000 U U O Spiral Plate Method O D 0000000 Reasoner D J and E E Geldreich 1955 A new medium for the enumeration and subculture of bacteria from potable water Applied and Environmental Microbiology 49 1 7 34 Zobell C E 1941 Studies on marine bacteria I The cultural
42. 0 D uuu D uuu 5 4 60 0000000060 5 4 61 0000000000000000000000000000000000 54 62 U 5 D uu 0 D uuu Lr t E E E LE LJ 57 000000 5 10000 0
43. 0000000 5 470000 5 10 8 000000000600 0 0 0 0 0 51090 00000000000000 una 0000 000000 000000 UDDDUD 000000 000000 0 00000000000000000000000 00000 20000090 0 000 0000 0000000 0
44. 0 0 0 0 4100 42000000 430000 440000000 450000000 460000000 470000000 4 8 00 49 0000000000000 4 10 0 0 0 U Bug 5 1 1 5200 5300000000 34 5 5 6 5 7 000000 5 8 00000000000 590000000000000 51000000 U Bau 1 1 43 r3 000 DU 000 Lr Lr Lr E ENE Ez Lr Lr L LT Lr Lr Lr Lr Lr Lr ERI Lr E J O LEE LIRE NE
45. 5447 4 12 2 2 000000 4 12 23 Lm 0O mn O mn O nr Lm m 4130000 4 13100000000000 0000000000000000000000 4 132 41400000000000 4 1410 0 0000000000000000000 000 000000000000
46. L1 L1 L1 L1 EJ Ea L1 E L1 L1 L1 L1 E L1 L1 L1 525 0 0 0 0 5 3 2 00 0 0 0 0 53 3
47. 4 4 4 4 4 59 45 00000000000 451000000000000000000000000000000000000 4 52 00000000000000000000000000000000000 45 3 00000000000000000000000000000000000 4 5 4 46 4 61 4 62 00000000000000000000000000000000000
48. 48 Bernner K P C C Rankin Y R Roybal G N Stelma Jr Scarpino and A P Dufour 1993 New medium for simultaneous detection of total coliforms and Escherichia coli in water Applied and Environmental Microbiology 59 3534 3544 49 U S Environmental Protection Agency 2002 Method 1604 Total coliforms and Escherichia coli in water by membrane filtration using a simultaneous detection technique MI medium Publication EPA 821 R 02 024 USEPA Official of Water 4303T Washington DC 35 MI medium No 3 Proteose peptone No 3 50g HU Hb Yeast eExtract 3 0 g B D 0 0 B D lactose 1 0g 4 6 4 Methylumbelliferyl B D Galactopyranoside MUGal 0 1g Indoxyl B D Glucuronide IBDG 0 32g NaCl 7 50 3 3 g 2 1 0g uuuuuunuunuunuun Sodium lauryl sulfate 0 2g Sodium desoxycholate 0 1g 00 Agar 15 0g uut Purified water 1000 6 95 0 2 121311 150 0000000000000 030 000 4 310 30000 040 D 47mml 050 0000
49. 0 6 55300 DU uuu E El Ez 5 5 4 000 5 5 5 0 0 0 0 r3 E EFER E L3 r3 E E E L3 r3 r3 r3 LT EE EE E LE EIT E 0 0 0 0 25 E L3 L3 L3 E3 E3 EJ EJ L3 L3 E3 E3 EJ ci L3 L3 E3 E3 EJ E3 E3 E3 EJ L3 L3 E3 E3 EJ E3 E3 E3 EJ Lr 0 2 EIEN ET EE E IEA L3 L3 E3 E3 EJ E3 E3 E3 EJ L4 E ETE r3 r3 0 0 556
50. 5 St 20000100000000000000000000 00000000 36 5 18 7 6000000000000000000000000000000 100m 5S50m 0 UIDDDDUDDUDDUDUDDUDUUDUDUBUDUUDDUDUDBDUDUDUDUDUDUDUDUDULU 3 8 L LI 36 U pH DOU NTU l 0000001 3 5 1 001 3 8 900000000000000 391 00000000000000 100000000 5060 000000000001 39 1I UUN 391 3 9 2 0000000 010 200000000000000000000 19 OOO 50 7000000000
51. 0 U intera Lr EES EI Lr Ld E E Lr Lr Lr p Lr E EIE E Lr Lr Lr Lr EI ES Lr Lr Lr Lr 1 69 D uuu D uuu D uuu 62 E E ETE EREI E71 E J E E l E ESI Ez AOA L3 L3 E3 E3 E3 E3 E3 E3
52. 4 1 8 Omura et al 2003 La mar 41 86 93 Guillard In Smith W L and Chanle y M H eds 1975 Culture of Marine Invertebrate Animals Plenum Press New York USA 26 e Q000 4 1 85 00000000 wel 1200000000000 60 90 pmol photons s0000000000000000 ep Viability QO 0000000000 100000000000000000 ODO OO viability 0 0 000000000000 O viability 0 000000 0000000 1000 004m DO00000000000 1000000 5000000000000 viability O 0000000000 500 00 Am 0000 415 00000000 DIII 2 0000000 000 20m0 00000000 2 27 4250000000 421 0000 N 4 2 1 10 0 D 011
53. 0 D2UUU D 210000 su p2l e 7 2060000000000 1000000 1800000000000 104000000 80 9 UU pot 100 000 000000000 D G8 6 00 0 2 3 3 0000
54. UUUUUUU OOOO 0000 3 Em 3 sana LEE 3 mace aen enn iat essent 3 EE WP REED EVERETSEDEDE M aen dn qa ete 4 2DDDDDDDDDDBDDUUDDBDUHUDBDHBHHUDUDDHD v00 5 SS uA E E E 5 S a E UR E 5 OE S RF HER ER Rt 6 Sl a a aun aa hapus LE Dd 8 SO SL IL 9 PH 9 9 uu 12 paqa apas 12 11 eesse 12 E no aene cats 14 RR 15 deer petris dom uto ed sr 15 E eodd 15 Rm 16 EC UAR E IR B D NC ROO 16 Ne REIN NR NERONE 16 avs LH Hl Silasta a na aa ettet utn 17 18 3 52 18 F6 BTPEEEBDE DLS 18 37DDDHDDDBDUUDDBDHBHDBDUBUDDUDHDUDDD eet ttem 19 SOM ce e e m m i M E pas 19 SETTE eoe da aet iD soa aita 19 310 QDO0000000
55. D U D 5 25 4 O ISO9001 2000 IU DD D D 1509001 20000 0 000000000 1509001 2000 60000000060 004100 4 140 O 00 00 51000 5 10000 00000000 4100 42 43 0000 44 0000000000000 00000 45 46 47000000000 48 00 49 00000000000000 4100000 4 11 0000 412 00000 4 13 0000 414 00000000000 5 1 D 52 53 00000000 54 5 5 5 6 00000000000 5 7 000000 5 8 000000 5 9 000000 5 10 00000 4 4 414 1 DISO IEC17025 DU DBDUUDDU 0000000 D uuu U uuu 13 5 2 0000 U
56. phyl um di vi si on 0 0 1 m 00 500 00000000000000000600 600000 1 m0 0 500 0000 0 D Uu I 00000000000000 11 2 1 Quality Assurance and Quality Control Procedures 2 1 1 The testing body performing the tests should have implemented appropriate quality control measures in accordance with recognized international standards acceptable to the Administration 2 1 20 The approval testing process should contain a rigorous quality control qualityassurance program consisting of
57. 00H E pg E H 15 3 000000 100000000000 310000 32 000000000 2 00001 300000000 3 2 00 000000000000 AUD 8 500 30000 001 3 2 1 1 3341 500000000 16 3235 331 1908000000 332 0000000 5 1 38 0 40
58. 020 i 000000000 20 0000000000 32 32 20 00000005000 1090 000000000000 3l 000 2 U UU 000000 000 300000000 50000000 1000t 00000000 000000000 10 0 4 3
59. cholerae O U O Coliform U L Enterococcus 2 UU toxiogenic Vibrio cholerae O1 39 Escherichia U U Interstinal Enterococei 8 OO0000000000000000000000 O0 23200000000000 32 4 3 1 00000 Heterotrophic bacterial 00000 010 00000000 00000 020 000000000 R24d nnm 2 11 Marine Agar 22161 DSMZ Medium6040 0 0 00000000000000000
60. U U Part 4 0 0 0 0 Resolution 126 53 Procedure for Approval of Ballast Water Management Systems that Make Use of Active Substances G9 23 00000000000 2 3 1 000000 2392
61. 24 cep Lr NephelometricTurbidity Unit 3 00000 3 1 1 D o0000001 0000000000680 1 3 2 O00 8 15 8 The Handbook of Standard Method For the Analysis of Water and Waste Water 9 SO standard methods 10 UNESCO standard methods 11 World Health Organization 12 American Society of Testing and Materials ASTM standard methods 13 U S EPA standard methods 14 Research papers published in peer reviewed scientific journals 15 MEPC paper
62. uu 1 5 d gt c eem ce e er t m 75 L3 r3 rr 112000 S050916 1 unknown Total S050916 2 HNF Total 5050916 3 Ca odiscus SP Vorticella sp Total 50916 20 nh 1 nhx 3 20 nh 1 nhx 3 Ter owh 1 0 27 1 L S050917
63. 534 000000 000 000 ES E ES E 5 L3 L3 E3 E3 E3 O 8 UJ O nr O n ET E ES FJ E EEE EI 1 Ez ESSE TE E EIE EE ENES L J E E Tip EEE ET ETE EINES DOD E3 EJ EJ ci L3 r3 E3 E3 E3 EJ E3 E3 EJ L3 r3 E3 E3 E3 EJ E3 E3 EJ L3 r3 L3 E3 E3 EJ E3 EJ E31 ETC EZ E23 EE 11k maulana lun lun luna ian lun 1 5 15 1 Ei 1 E ET E 5 15 ENE ETTI ES EST ESI Ed ES ES s ESI EJ maiaa ua lun 5 15 E r E ES 5 15 5 Ez
64. P 3 2 Chaetoceros affinis Chaetoceros curvisetus Chaetoceros sp Ceratium furca Skeletonema costatum Total 85 051112 1000 ml No 19 80 ml ml P 3 3 Actinoptychus senarius Ceratium furca Chaetoceros curvisetus Chaetoceros s Cochlodinium sp Coscinodiscus sp Ditylum brightwellii Skeletonema costatum Thalassiosira sp Total 14 140 89 05 20 94 2 2100000000000 11 30 Moo mr di nensi on Coscinodiscus sp 9 S0pm 22 2 a Ciliophora 2 3 100mm Coelenterata planula 2 200pm Calanus sp 532 j 300m Paracalanus 20 0300 Paracalamus parvus__ 8 oO 200pm Centropages sp 20 300pm PPseudodiaptomus sp 41 300p
65. uou Coscinodiscus sp 4 S0pm uM o Synchaeta sp S0pm Calanus sp 9 oa sp 23 29140 Saphirella like copepod 3 60 120 m Calanus copepodite e0pm Oithona copepodite 2 100pm Copepoda nauplius 32 100pm Polychaeta larva Jo l1l 80 20m Polychaeta trochophora 7 20m Pelecypoda umbo J 6 5060um pu Lee ce o i EN Total _ 1920 93 ous 00000 C ms C mm C L gt CU L3 r3 wO BL H a n NJ LU LLL 02 45 Protoperidinium sp Coscinodiscus sp _ 1023 Total T 204600 T c cA S I EIE 5 9 s 5 A 94 2300000000 00 NO
66. 0 0 0 0 0 0 0 0 0000000000 71528402 0 000000000000 L3 L3 E3 E3 EJ E3 E3 E3 E3 EJ to uu A A SS E 5 4 7 2 DU Uu L1 r3 r3 LJ E EIE r3 r3 r3 EIE E CY O O L1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0000 conf i grati a ESI ES ES ESPERE ES E E ESL EST ESI ESI OU ESL ES Ez Es ES ESL ES pRLEICIE D 02 EJ a C3 EH EJ E3 kum EJ E3 q um F3 F3 e E Ed EJ EJ E3 EJ E3 F3 E EJ EJ E3 EEE L3 L3 E3 E3 E EE ET 5 51
67. 2 DO 0800 000 25 a NaNO 1x 10 NaH PO 1x 10 5 10 ZnSO4 7H O 1x 10 4H20 1x 107 NaMoO4 2520 5 107 6H20 2 107 CuSO4 520 1x 103 Na EDTA 2H20 2 4 10 25601 2 107 Thiamin 5 93x 107 0000 Biotin 4 1 x 10 Cyanocobalamin 738x 10 000000 mol tp b 20000 NaNO 8 83x 10 NaH PO 3 63x 10 Nasio 9H20 1 07 10 FeCl 6H20 1x 10 Na DIAU 1x 107 CuSO4 5H0 4 10 NaMoO4 2H20 3x 10 00000000 718040 7H O 8x 103 690 5 10 490 9x 107 Thiamin 1 3 107 0000 Biotin 2 10 00000000 Cyanocobalamin 1x 107 000000 mol 2 24 welt nnt 0000 1mtl
68. 43 0 40 000000000 43 3 20 200 100m0 000000000 N D Not detectable 0 035 2 C U 18 24000000000 Del 00000 Uv 36 36 4 3 3 1 D D D 0 U Enterococcus O U Intestinal Enterococci 0000 0000000000 4321 020 000000000 0 0 Enterococcus Agar m Enterococcus Agar Azide D DDD D 000000000000 Pancreatic digest of casein 15 0g Papaic digest of soybean meal 5 0g 00000 Yeast extract 5 0g KH PO 4 0g 0000 Glucose 2 0g uultu NaN 0 4g Triphenyltetrazolium chloride 0 1g 00 Agar 10 0g uu Purified water 1000m t pH72 02 030 000 4 310 30000 040 0000 43 0 40000 43 3 50000 00000 37 2
69. 5 62 22 2 5 6 21 2 5630000000000 56310000 0000562100000 56320000 5633000000 5634000000 57000000 5710000000000 T5E E 3 EI E23 L3 L3 E3 E3 E3 E3 N m
70. O 10 TSS 04um0 00000000000000 60 0000 1000 3 2000000000000000 0000 0607040 200000 01mg D D utu L3 r3 o r3 o 0 0 3l 0 0 00 0 41 35 100000 357 D U D 100 20mCH 3040000 6 eq 208 000000000600 80 55 000000000 TSS mg l W W V 20 DOC 45oq 07y m0
71. 1600mt 000001 3 9 10 08000 00 50um p D DE D 20 0 TB D UD D U D U 391 020 030 59 21 20um LI D DI 00 1 3 92 71 O O O Sefar O OOOO D 35 20 040 50000000 remcp po DDD L1 310 21 1 4 D D D viability 4ILDDODULUDUU 4 1 1 00080
72. 332 1 0 pm 001 3 3 2 020 030 33 3 001 3 3 D B O Sefar O OO O O O NY7 HD Super 17 040 050 1 pe 34 DUDDUD UU UU 34 1T UU JN 341 35
73. 65 9 1043 5 104 4 5 10 5 000000 I S I EC 170200 0 D Uu 0 D uut 5 106 D Uu 5 107
74. Protoperidinim sp 1 Thalassiosira sp _5 Wesellasp Jh E _ puo i c fi 87 051112 1000 ml 27 5 ml S 2 1 00 50 Bacteriastrum sp 1 Ceratium furca 1 Chaetoceros affinis Chaetoceros curvisetus Chaetoceros spp Coscinodiscus spp 69 Ditylum braightwellii 1 Oxyphysis oxytoxoides 2 Protoperidinium spp 3 Scrippsiella sp 1 Thalassiosira sp 16 Total 336 051112 1000 ml 27 5 ml S P 2 2 Chaetoceros affinis Chaetoceros curvisetus Chaetoceros sp Ceratium fusus 3 Cochlodinium sp 1 Coscinodiscus sp 2 Skeletonema costatum Total 111 Prorocentrum 051112 1000 ml 27 5 ml S 2 3 03 40 Ceratium furca 2 Ceratium fusus 3 Chaetoceros affinis Chaetoceros curvisetus Cha
75. SHE ogoan g L3 OOOO Y E3 EJ EJ EJ EJ EJ EJ EJ p EJ C3 C3 C71 E3 E3 EJ EJ E3 F3 lt EJ 00 nmn4 1 0000 1n o i9 Bacteriastrumfurcatum Bacteriastrum sp Chaetoceros sp Guinardia sp Nauplius Odontella sp Protoperidinium sp Tintinngusis lobiancoi 9 5 m Iu o 50m jdinium sp Thalassiosira sp Tintinnopsis sp unknown Total inunnaund 50m 0A o 1 S o i o 9 o i o QG r B 313 333
76. ma 375m 53 85 4 NIE ing Mlexandrium sp j 1 ICochlodinium sp 2 j Ditylum brightwellii Steleronema comm __ o 5 Thalassiosira sp punn 051111 LLL m SEITE 1000 mi UUDDDDD 375m s0000 e 0000 M2 _ ms ep WW Ditylum brightwellii rr HH 051111 L JUUN n 375m 0000 1m 50000 000000 M3 00 50 Cochlodinium sp Ditylum brightwellii Protoperidinium sp E m coge p Surirella sp El 1 j jJ y Protozoa 3 6 Skeletonema costatum gt Ca 4E Thalassiosira sp 103 f j y Surirella sp po 1 I y Toat 5 10 um Cyclotella sp Scenedesmus sp No 12 uum 1 oof P Ditylum braightwellii Planktoniella blanda 1
77. 48 3 0000000 050 00 43 5 000000 65 4505 48 1000 19000000000 50 51 50 American Public Health Association 2005 Standard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 51 U S Environmental Protection Agency 2002 Method 1600 Enterococci in water by membrane filtration using membrane enterococcus indoxyl B D glucoside agar mEI Publication EPA 821 R 02 022 USEPA Office of water Office of Science and technology USEPA Washington DC 5 Slanetz L W and C H Bartley 1957 Number of enterococci in water sewage and feces determined by the membrane filter technique with an improved media Journal of Bacteriology 74 591 595 37 4 3 II 4 3 6 T D Brain Heart Infusion Broth Pancreatic digest
78. F L3 L3 L3 E3 EJ E ECT E L3 L3 E3 EJ L3 L3 E3 E3 EJ ET ET E71 E p E EJ E3 EZI E tJ ELEN EI L3 L3 L3 E3 EJ LEI a L3 r3 r3 EERE L1 E L1 L1 L1 L1 EET ESET L3 L3 L3 E3 E3 1 Esp Esp Ed E L3 L3 L3 E3 EJ E3 EJ L3 L3 L3 E3 E3 EJ E3 EJ LI EA ETE TTE EF EEA Uj i ET EST ESI E Ez EISE ESI EIEN EJ ET ETE E E E L3 rrr L3 L3 L3 E3 E3 EJ L J JJ J rJ J D D w EE O ma I maa iq usan E L3 r3 1 L3 L3 E3 E3 E3 E3 E3 E3 E3 EJ E3 E3 E3 EJ EJ E 3 EET E TET EAE E L3 L3 E
79. 4 2 1 00000000000 2 10 50 UUDUUDUDDUDUDUDUDUDUDUDUDDUDUUDUUDDUUDUUDUUuDUuLD 10 50m0 0 50pm 0 0 10 mc D D rep 15 35 0 Do D Ud BUD UU mcn Du 45 135 210 mt 0 D 4 0 4 22 minimum dimension O O 0 12000 423 000000000000000 minimum dimension 0 0 0 4 1 3000 424 viability O O O O O 28 4 1 4 425 00000000 41 5000 29 41 1
80. cfu nm Marine 23 11700 30800 16220 39800 61000 R2A 2684 5760 4240 2290 1060 428 11800 504 534 860 0 340 0 44 10 ND O1 O1 ND ND ND amp 6 2 40 28 36 380 6 5 aCl broth 46 26 26 32 300 D D cfu 100m XM G agr 23 C 6 D D J 3460 2480 44000 4460 19600 cfu 100m XM 23 61 0 0 0 16 12 4 8 20 NDI O O 24000000000 05 5 2 10 ml 00 7 000000000000 170 200 0000000000 92 0000 000000000000000
81. 14 LIER E LEE Jis 2 9901 1998 1 JIS 2 9902 1998 000 JIS 2 9901 1998 JIS 2 9902 19980 00 0000000000000 0 5 2 9901 1998 JIS Z 99021998 2 I SQI EC 17025 1999 General requirenents for the conopetence of testing and cal i brati on aboratori es DI 00 0 0 0 0 U uu 0 0 0 0 0 5 2 9358 4 13 0 0000000000000 D uu 0 000000000 00000 15 2 93580 E HH 0 HH HH HH p HH HH p HH EET p HH p HH HH 0 HH ER EE rj EH HH
82. 4 Urmbilicosphera sp Vorticella sp Total 1 nhx 3 11 5050917 5 Umbilicosphera sp Acting sp unknown Total 20 nh 1 nhx 3 10 10 36 regrowth 9 27 lie regrowth 9 27 2 regrowth 9 27 14 0 0 0 1 L S050917 6 Acting sp Total 20 nh 8 1 nhx 3 76
83. N r3 m o0 ON o G e O ooog o7 nimm r3 5 r3 r3 EE e EJ aeg QQ m4 umi LL EE 1 oo zu mulu 5 mas E E ol vc rn Cc iu m fun r3 Emtec EE aga 20 oOo Goon Egon AAO oon aaa oo AAA Q og N r3 mm 95 EE m lt oooogooon aa aaa DEB doocogtboooooooooggsooognbooooooooooooooooono EE Og as aaa aaa 1 u G B lt 1 rn o O manu oono ra o0 68 3 0000 uu BEL 0000 9100600 00000 T uem 69
84. p HH 0 HH p HH Ej 0 00000000 4100 411 44 412 00000000000000000000000000000000000 4 1 5 D uu 4140 0 000 00000000000000000000000000000000 0 415 000000000000000000 al
85. requirements of heterotrophic aerobes Journal of Marine Research 4 42 75 35 German Collection of Microorganisms 33 030 000 7 27 Phosphate buffer solution dini KH5PO 42 5mg MgCl 6H20 190mg mog Purified water 1000 m amp ttu a H 0000 3420000 5eomtcp D C Olmo D D C U 0000000 7 2 05 0 00000 b 6000000000 3880000 1000me 0 nm p D e 000000 12500000000000 Somt U O 1000mt 121 1 150 0000000000000 040 0000 50 4 3 0 000000000000 00 DU 0000 D uu 01 43 1 36 40 36 JIS K 0430 72 50 2000 5 6222 1988 37 ISO 6222 1988 Water quality Enumeration of viable micro organisms Colony count by inculation in or on a nutrient cluture medium 38 ISO 9308 1 1990 Water quality Detection and enumeration of Escherichia coli and coliform bacteria Part 1 Membrane filtration method Phosphate buff
86. 000 000000000000 6000 6 ox 55 50mgL II HDD UU UUDUDUDUDUDDUDDUDUDUDUUDUUDGUUDDUGuUDDUDUUUDU L1 L1 L1 L1 L1 E LIO 3300000000000 04 lu UU Viable organisms 0 414 U U viability 0000000000000 4 Q5 A 4 1 3 96 1100000 19017025 0 0 0 150170250000 1 UUUUUUUU QMPO 45D D EJ p EJ p pH Ol o oa A GOM aoa A Ol 0O Ol ga 0O OID a A HI 2000000000000000000
87. 0000000000000 1m0 0 D00000 D 100000000000000 5 10m0 0000 5 1 100000 522 00000000000000000000000000000000 1 0000000000 0 100 0 1390 000000 1 cfu 100mt J cfu colony forming unit O 1 efvig 2 00000000 250 cfu 100 mt 3 000000000 100 cfu 100mt O IDM 4 1 2 0 O I 4 1 3 0 O O 4 1 4 0 O O gt 2 21 00000000 20050 70 000000 00000000000000000000000 O UOMO 2 2 80000 4000000000 000000000 HBD o Pet 1 0
88. 000000000000000 un 21 22 22 A2 STI REDE 28 E RE 32 41 NR ER TR IE RR 43 xL rta 43 002 HDDDHDDDHBHBDDDUHDBDDHBDD ote et Ta tva 67 apa a 69 Top EL DERE BEES BIET E ER RE RS ve DECR SP ona 5 00 20040 209 00000000 0000000000000 000000 2 7 52
89. 3 E3 E3 E3 EJ Oooo Ea E L3 r3 r3 E ESTE LEN LENE EJ EET ERAN EI J E E E 0 D uu 0 L E E 5 L3 r3 r3 r3 r3
90. 4 2000 poc b Dt 20 0 00000000 0000 00000000 0000000 O00 eRD 2 000000000000000000 pop 1 69 0 3 2 2020 00000000000000000000000 2 soq 4 31 L 4 sEFDULU U DOC pH 42 1 ISO IEC 17025
91. EIEN E3 E3 EJ E3 E3 E3 EJ LT EL EST L ET ET EE ET E21 ES EST E73 L r E EST E EST ESI ESTIS EET ED AEST ET EI EIE EI II E3EA E EI STE E e EIE EE E E EIE EE ERE EIE E EL ESI EIS ET ES EST EIS ET i E E EI EST E E ED Lr Lr L3 L3 L3 E3 E3 E3 EJ EJ B T ET EN ELIT ELE ET p AES ST E ETE EJ E p E E EES Ez L3 L3 L3 r3 r3 HE g 5 5 5 EE EJ EH EXE EI oonga 0 0
92. Lr Lr Lr Lr 63 al 60 000000006000 000000000000000000000000 d 0000000000000000 aj 5 10 00000 5 10 1 5 10 21 5 10 300 5 10 5 10 20 0 5 10 408 000000000000000000000000000600 0
93. cylinarus aanicus 6 12 bero dea 25 40 Skeletonema costatum 6 22 Tintinngpsis corniger 28 33 Cerataulina pelag ca 7 56 Tintinnapsis radix 30 43 Chaetoceras danicum 8 20 Salpingella sp 30 50 Rh zosolen a fragilissima 8 70 Tintinnapsis 35 38 Chaetoceras affine 9 30 Amphorella quadarilineata 40 46 Chaetoceros debile 9 40 Tintinnapsis directa 40 47 Chaetoceros didymum 10 40 lohmanni 40 62 Chaetoceras lorenzianum 10 80 L eotocylinarus mediterraneus 10 35 Mesodinium rubrum 23 30 Detonula pumila 12 55 Tiarina fusus 30 35 Chaetoceras constrictum 14 35 Actinoptychus senarius 20 105 Trichocerca sp 28 50 Eucamp a zod acus 20 100 Thalassiosira rotula 20 60 Euglypha sp 30 60 Prorocentrum m nimum 15 23 Protoperidinium bipes 15 20 Heterocapsa triquetra 17 29 Gonyaulax verior 26 32 Ceratium furca 30 50 Distaohanus speculum 15 35 Ebria tripartita 34 40 000000 akash wo 6 15 31 4 3 cap 23509000000000000000000000000000000 3 2320
94. er solution 39 Standard Methods for the Examination of Water and Wastewater 21th edition 2005 American Public Health Association Washington D C Buffered water 40 Standard Methods for the Examination of Dairy Products 17th edition 2004 American Public Health Association Washington D C 34 050 20 2000 000000000000 M 3 2 43 2 0000000000000 4 3 2 U U Coliform O U U U U U U Escherichia colit E U U 0000000000000 000000000000000000 020 00 MI medium J O O O O D 41 46 41 American Public Health Association 2001 Compendium of methods for the microbiological examination of foods 4th ed American Public Health Association Washington D C 42 American Public Health Association 2005 Standard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 43 U S Food and Drug Administration 2000 Bacteriological analytical manual 8th ed International Gaithersburg Md 40000000 200508 00000000 2005 400000000 200408 000000 0000 0000 0000006060 U UU ULL U 20010
95. etoceros sp Cochlodinium sp 1 Corethron sp 1 Ditylum brightwellii 3 Protoperidinium sp 1 Total 143 Prorocentrum 88 No 13 1 ml 01 45 5 4 19 8 218 4 No 14 1 ml 4 8 92 1 15 1 ml 5 19 108 051112 1000 ml 17 80 ml ml P 3 1 04 40 Chaetoceros affinis Chaetoceros_sp Cochlodinium sp Dactyliosolen sp Protoperidinium spp Skeletonema costatum Thalassiosira sp Ciliata Tintinnopsis sp Total Prorocentrum 122 051112 1000 ml 05 50 2 16 6 95 3 6 No 18 80 ml ml
96. ia O0 00000000 20 28 5 70 0 000 0 0 ION O 4 14000 gogig 41200 413000 18 25 18 Bacteriological analytical manual 8th ed AOAC International Gaithersburg Md 19 Clinical microbiology procedures handbook American Society for Microbiology Washington D C 20 Compendium of methods for the microbiological examination of foods American Public Health Association Washington D C 21 International Organization for Standardization 22 Standard methods for examination of dairy products American Public Health Association Washington D C 23 Standard methods for examination of water and wastewater American Public Health Association Washington D C 40000000 200558 00000000 2005 2500000000 20040 000000 0000 0000 10 50 10000 phlumdi vi si 5000000000 2 000000 10 Sum 0000 5 000 UU 500000 0 0
97. m Labidocera sp 3 j 300pm Temora discaudata 3 30m Oithinabrevicornis 4 I20pm Oirhina simples 1_j Oithona sp 50 l 20m Microsetellanorvegica 16 100 160 Saphirella like copepod 65 60 120m Harpacticoida 3 300pm Calanoida copepodite 1 8 100200 Paracalanus copepodite 4 50300 Centropages copepodite ll 200pm Pseudodiaptomus copepodite 35 200pm Labidocera copepodite 1 300pm Oithona copepodite 4 100 Corycaeus copepodite 3 I20pm Microsetella copepodite 2 1 50 Copepoda nauplius _ 376 100pm Isopoda 1 Brachyura zoea 1 300pm EI MEM CRM Tol Toa g d 276 90 LI 0 Z gt 3 r3 5 91 osim ES 100 92
98. of gelatin 14 5g Brain heart solids from infusion 6 0g uU uuu Peptic digest of animal tissue 6 0g NaCl 5 0g Na HPO 2 5g uu Purified water 10004 pH 7 4 0 2 121 1 4 3 4 Vibrio cholerae D I U D U U D I toxiogenic Vibrio cholerae O1 and O139 00000 DU uu U uu D uuu 010 0000000 00 Salts Sucrose Agar 0 O O O O 15 4 3 21 0 0 Thioesulfate Citrate Bile U U 10 056 Yeast extract 5 0g Sodium citrate 10 0g Na2S203 10 0g NaCl 10 0g uU uuu Ferric citrate 1 0g uU DU Oxgall 5 0g UUUUUUU UU Sodium cholate 3 06 utu Sucrose 10 0g 53 55 53 American Public Health Association 2005 Standard methods for examination of water and wastewater 21th ed American Public Health Association Washington D C 54 U S Food and Drug Administration 2000 Bacteriological analytical manual 8th ed AOAC In
99. ona davisae 150um ish larva 400um bL Total T 62 3 5 80 9 60 Podon schmackeri 120um 9 3 80 050918 150 S 0509186 Total 0 050918 11 20 13 10 Coscinodiscus 31 60 90 opepoda nauplius 60 Coscinodiscus ogranii 5O Toal 81 Harpacticoida copepodite 200um 1 Lor Toal 2 2 j L 82 15 1505 150 L 0509160 L3 uw
100. tems G8 O 102 0094 3 321 03 326 66 000 EJ EILEEN EST E LE EEE FT L1 ETE EIETIE J E 000 0000000 00 JU 0 BU UUDUDUDUDUDUDUUDUDuUpuDu 00 000 UUDDUDUDDUDUUUDUuDDUUDUUUUU u 0 DU 0 r3 DU 00 00 I 00 000000000000 L1 000000000000
101. ternational Gaithersburg Md 5 20040 000000 0000 0000 00000000 7 5 0 000000 0000 0000000 18 387 391 38 0 299 000000 0 296 Thymol Blue 20mt 0 294 000000000 0 296 Bromothymol Blue 20mt 15 06 ut Purified water 1000m 8 6502 20 000 4 3 1 3L 0000 30 0000 4 3 10 400 43 20 5000 000000 361 24x 2 1 000000 4 43 4 U 4 3 20 60000 U UH 4 3 4 U 020 000000000000 10 36 1 6 1 00 000 0 43 5 Peptone 10 0g uu Purified water 1000m 8 640 2 121551 39 4 3 5 211 000000000 0 000 0139
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